A time difference culture device

Abstract

The present invention discloses a time difference culture device, which relates to the technical field of biological tissue culture; the time difference culture box is adapted to be equipped with a light source assembly, an observation assembly and a gas supply system; the light source assembly includes a first light focusing part, a light decaying sheet is arranged on the light incident side of the first light focusing part, and the light decaying sheet is provided with a plurality of light transmitting parts in sequence along the length direction of the light decaying sheet, and the light transmittance of the plurality of light transmitting parts increases in sequence along the length direction of the light decaying sheet; the observation assembly includes a microscope camera and a linear drive mechanism; the gas supply system includes a first pressure switch, a first reversing pressure relief valve and a first gas storage tank, the first pressure switch is used to monitor the air pressure in the time difference culture box and control the action of the first reversing pressure relief valve, and the outlet of the first gas storage tank is connected to the inlet of the first reversing pressure relief valve after passing through a pressure reducing valve. The present invention can ensure the clarity of the captured image, and the image has a three-dimensional sense and a sense of hierarchy, so as to accurately judge the morphology of biological tissues and ensure the accuracy of biological tissue evaluation, analysis and screening.

Description

Technical Field

[0001] The invention relates to the technical field of biological tissue culture, and in particular to a time difference culture device. Background Art

[0002] In vitro fertilization (IVF) refers to the technology of fertilizing mammalian sperm and eggs in an artificially controlled environment outside the body. The current in vitro culture of human embryos is achieved by placing the culture dish containing the embryos in a time-difference incubator or a conventional carbon dioxide incubator for culture, while ensuring the temperature, humidity and cleanliness of the culture environment.

[0003] Among them, the time-difference incubator can provide a relatively closed culture and development environment for the embryo that is close to the mother's uterus, and can realize real-time monitoring and control of the temperature, CO2 and O2 concentrations, and gas pressure of the culture environment. At the same time, a microscopic photography system is required. When the incubator is closed, the embryo images are taken at regular intervals to fully record the development status of the embryo at each stage, and the embryo morphological parameters are identified through software image algorithms, so as to complete the evaluation, analysis and screening of the embryo.

[0004] In the existing time-difference incubator, the lighting lamp is guided to the culture dish through a focusing system to provide light for the microscopic photography system. Although it can provide sufficient light and avoid light damage to the embryo, the pictures taken by the microscopic camera are flat pictures, lacking three-dimensionality and layering, making it difficult to accurately determine the morphology of the embryo, affecting the accuracy of embryo evaluation, analysis and screening. Summary of the invention

[0005] Aiming at the technical problem that the existing time-difference incubator uses a focused lighting lamp to observe embryos, the obtained image lacks three-dimensionality and layering, and it is difficult to accurately judge the morphology of the embryo; the present invention provides a time-difference incubation device, which can make the biological tissue image obtained by a microscope camera have three-dimensionality and layering, so as to accurately judge the morphology of the biological tissue and ensure the accuracy of the evaluation, analysis and screening of the biological tissue.

[0006] The present invention is achieved through the following technical solutions:

[0007] A time difference culture device comprises a time difference culture box, which is adapted to be equipped with a light source assembly, an observation assembly and a gas supply system; the light source assembly comprises a first light focusing portion, a light decaying sheet is arranged on the light incident side of the first light focusing portion, the light decaying sheet is provided with a plurality of light transmitting portions in sequence along the length direction of the light decaying sheet, and the light transmittance of the plurality of light transmitting portions increases in sequence along the length direction of the light decaying sheet; the observation assembly comprises a microscope camera and a linear drive mechanism, and the linear drive mechanism is used to drive the microscope camera to move along the axis of the first light focusing portion; the gas supply system comprises a first pressure switch, a first reversing pressure relief valve and a first gas storage tank, the first pressure switch is used to monitor the air pressure in the time difference culture box and control the action of the first reversing pressure relief valve, the first gas storage tank is used to store culture atmosphere gas of a set concentration, and the outlet of the first gas storage tank is connected to the inlet of the first reversing pressure relief valve after passing through a pressure reducing valve.

[0008] When in use, the present invention connects the first pressure switch and the first reversing pressure relief valve to the time difference culture chamber respectively, pressurizes the culture atmosphere gas (oxygen and carbon dioxide mixed gas) of a set concentration in the first gas storage tank, and places the culture dish containing biological tissue and culture fluid in the time difference culture box; then adjusts the pressure reducing valve so that the gas in the first gas storage tank is output according to the set pressure, and the pressure-regulated gas is input into the time difference culture chamber through the first reversing pressure relief valve, and the air pressure in the time difference culture chamber is monitored in real time by the first pressure switch. When the air pressure in the time difference culture chamber is higher than the set upper limit value, the first pressure switch controls the first reversing pressure relief valve to reverse so as to relieve the pressure of the time difference culture chamber. When the air pressure in the time difference culture chamber is lower than the set lower limit value, the first pressure switch controls the first reversing pressure relief valve to reverse so as to input the culture atmosphere gas into the time difference culture chamber, so that the gas pressure in the time difference culture chamber is maintained at the set value.

[0009] When it is necessary to observe biological tissue, the light source is provided by the light source assembly, and the biological assembly is observed in real time by the observation assembly. Since the present invention is provided with a light attenuation sheet on the light incident side of the first focusing portion, the light emitted by the light source lamp can be attenuated to reduce the light damage to the biological tissue by the light source. A plurality of light-transmitting portions with successively increasing transmittances are sequentially arranged on the light attenuation sheet along the length direction of the light attenuation sheet. The light emitted by the light source lamp can be formed into gradient light and then focused to the biological tissue observation area of ​​the culture dish through the first focusing portion, so that a three-dimensional and layered image of the biological tissue can be captured by a microscope camera, so as to accurately judge the morphology of the biological tissue and ensure the accuracy of the evaluation, analysis and screening of the biological tissue.

[0010] Among them, a plurality of light-transmitting parts with increasing light transmittance are sequentially arranged on the light-decaying sheet along the length direction of the light-decaying sheet, which can not only form light with various light intensities, but also make the light intensity irradiated on the biological tissue meet the imaging requirements. Therefore, the present invention can make the biological tissue image obtained by the microscope camera have a three-dimensional sense and a sense of layering, so as to accurately judge the morphology of the biological tissue and ensure the accuracy of the evaluation, analysis and screening of the biological tissue.

[0011] In an optional embodiment, a second light focusing portion is further provided between the first light focusing portion and the light attenuation plate, and the volume of the second light focusing portion is greater than that of the first light focusing portion, so as to improve the utilization rate of the light from the light source through two-stage focusing, and further ensure that the intensity of light irradiated on the biological tissue meets the imaging requirements.

[0012] In an optional embodiment, the incubator bottom plate of the time-difference incubator is rotatably connected to a rotating shaft, the upper end of the rotating shaft is used to support the culture dish, and the rotating shaft can rotate around its own axis; an observation channel is provided in the middle of the rotating shaft, and the microscopic camera moves along the axis of the first focusing portion in the observation channel. When in use, the culture dish is placed on the upper end of the rotating shaft, and the culture dish is driven to rotate by the rotating shaft, so that the culture dish can be rotated relative to the microscopic camera in the time-difference incubator to scan the circumferentially distributed biological tissues. At the same time, the microscopic camera is driven by a linear drive to move along the axis of the rotating shaft in the observation channel, so that images of multiple focal planes can be collected, and repeated focusing of the microscopic photography system can be avoided, thereby improving the focusing efficiency and ensuring the clarity of the captured images.

[0013] In an optional embodiment, the outer wall of the rotating shaft is provided with a first sealing ring, and the first sealing ring is used to seal the gap between the rotating shaft and the bottom plate of the incubator. The outer wall of the first sealing ring is provided with a spring clamp ring, which can, on the one hand, make the first sealing ring tightly attached to the outer wall of the rotating shaft to prevent gas leakage in the incubator, and on the other hand, can timely make the first sealing ring body tightly attached to the outside of the rotating shaft after the first sealing ring is worn, so as to prevent the first sealing ring from failing in sealing during use.

[0014] In an optional embodiment, the linear drive mechanism includes a lead screw, a slider and a slide rail, and the microscope camera is fixed on the slider. The lead screw and slider mechanism drives the microscope camera to move along the axis of the rotating shaft, which can maintain the position of the microscope camera through the self-locking of the lead screw and slider mechanism, and ensure the movement accuracy of the microscope camera.

[0015] In an optional embodiment, a driving block and a supporting block are fixed on the sliding block at intervals, the driving block is threadedly connected to the lead screw via an anti-backlash nut, an anti-backlash spring is arranged between the anti-backlash nut and the supporting block, and the microscope camera is fixed on the supporting block so that the thread of the anti-backlash nut is tightly attached to the thread of the lead screw to prevent transmission gap during the transmission of the lead screw, so that the microscope camera does not lose steps under the control of a conventional stepping motor, thereby ensuring that the microscope camera moves according to the set distance, thereby ensuring the clarity of the shooting.

[0016] In an optional embodiment, a second reversing pressure relief valve is connected between the first gas storage tank and the pressure reducing valve to control the maximum output pressure of the first gas storage tank through the second reversing pressure relief valve.

[0017] In an optional embodiment, a second gas storage tank is connected between the pressure reducing valve and the first reversing pressure relief valve to provide a buffer for the gas flowing out of the outlet of the pressure reducing valve through the second gas storage tank, so as to stabilize the intake pressure at the inlet end of the first reversing valve and reduce the operation frequency of the first reversing pressure relief valve, thereby saving culture atmosphere gas.

[0018] In an optional embodiment, the second gas storage tank is connected to a second pressure switch, and the second pressure switch is used to monitor the pressure of the second gas storage tank and control the action of the second reversing pressure relief valve to prevent the gas storage pressure of the second gas storage tank from being too high.

[0019] In an optional embodiment, there are multiple time difference incubator arrays, and the multiple time difference incubators are fixed on a rack to culture and observe multiple groups of biological tissues at the same time, so as to improve the culture and observation efficiency of the biological tissues.

[0020] The present invention has the beneficial effects:

[0021] 1. The present invention adjusts the pressure reducing valve so that the gas in the first gas storage tank is output according to the set pressure, and the pressure-regulated gas is input into the time difference culture chamber through the first reversing pressure relief valve. The first pressure switch monitors the air pressure in the time difference culture chamber in real time and controls the reversal of the first reversing pressure relief valve to input the culture atmosphere gas of the set concentration into the time difference culture chamber to maintain the gas pressure at the set value, which can provide the time difference culture chamber with an atmosphere of stable concentration and pressure to meet the needs of embryo culture, and has the characteristics of simple structure and simple control.

[0022] 2. The present invention has a light attenuation sheet on the light incident side of the first focusing portion, which can attenuate the light emitted by the light source lamp and reduce the light damage of the light source to the biological tissue. A plurality of light-transmitting portions with successively increasing light transmittances are arranged on the light attenuation sheet along the length direction of the light attenuation sheet. The light emitted by the light source lamp can be formed into gradient light and then focused to the biological tissue observation area of ​​the culture dish through the first focusing portion, so that the biological tissue image with three-dimensional and layered sense can be captured by a microscope camera, so as to accurately judge the morphology of the biological tissue and ensure the accuracy of the evaluation, analysis and screening of the biological tissue.

[0023] 3. The present invention drives the culture dish to rotate by a rotating shaft, so that the culture dish can rotate relative to the optical component in the time difference incubator to scan the circumferentially distributed biological tissue. At the same time, the linear drive drives the microscope camera to move along the axis of the first focusing part in the observation channel, and can perform image acquisition on multiple focal planes to avoid repeated focusing of the microscope photography system, improve focusing efficiency and ensure the clarity of the captured image.

[0024] 4. The present invention arranges a spring clamp ring outside the first sealing ring, which can make the first sealing ring close to the outer wall of the rotating shaft to prevent gas leakage in the incubator, and when the first sealing ring is worn, the first sealing ring body is promptly close to the outside of the rotating shaft to prevent the first sealing ring from failing in sealing during use.

[0025] 5. A second gas storage tank is connected between the pressure reducing valve of the present invention and the first reversing pressure relief valve, so as to provide a buffer for the gas flowing out of the outlet end of the pressure reducing valve through the second gas storage tank, so as to stabilize the intake pressure of the intake end of the first reversing valve and reduce the operation frequency of the first reversing pressure relief valve, thereby saving the culture atmosphere gas. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0027] Figure 1 This is a schematic diagram of the structure of the time difference culture device according to an embodiment of the present invention;

[0028] Figure 2 It is a structural schematic diagram of an incubator of a time difference incubation device according to an embodiment of the present invention;

[0029] Figure 3 It is a schematic diagram of the structure of an observation system according to an embodiment of the present invention;

[0030] Figure 4 This is a schematic diagram of the structure of a light source assembly according to an embodiment of the present invention;

[0031] Figure 5 A schematic diagram of the structure of a light attenuation sheet according to an embodiment of the present invention;

[0032] Figure 6 Schematic diagram of the structure of a microphotographic assembly according to an embodiment of the present invention;

[0033] Figure 7 This is a schematic diagram of the structure of a culture dish drive assembly according to an embodiment of the present invention;

[0034] Figure 8 for Figure 7 A magnified schematic diagram of part A;

[0035] Fig. 9 This is a schematic diagram of the structure of a first sealing ring according to an embodiment of the present invention;

[0036] Fig.10 This is a schematic diagram of the structure of a second sealing ring according to an embodiment of the present invention;

[0037] Fig.11 Schematic diagram of pipeline connection of the gas supply system of the incubator according to an embodiment of the present invention.

[0038] Reference numerals:

[0039] 100-Petri dish;

[0040] 200 - bottom plate of incubator, 210 - support plate of culture dish, 220 - rotating shaft, 221 - observation channel, 230 - first sealing ring, 231 - spring clamping groove, 232 - first annular protrusion, 240 - spring clamping ring, 250 - second sealing ring, 260 - third sealing ring, 261 - second annular protrusion, 270 - second rotating driver, 271 - synchronous belt;

[0041] 300-linear driving mechanism, 301-lead screw, 302-sliding block, 303-sliding rail, 304-driving block, 305-supporting block, 306-anti-backlash nut, 307-anti-backlash spring, 308-first rotary driver, 309-spring fixing groove, 310-microscope camera;

[0042] 400-light source assembly, 401-first light focusing part, 410-light attenuation plate, 411-light transmission part, 402-second light focusing part, 403-installation tube, 404-light inlet plate, 405-step hole, 406-light source lamp, 407-fourth sealing ring, 408-cover plate;

[0043] 500-time difference incubator, 501-first pressure switch, 502-first reversing pressure relief valve, 503-first gas storage tank, 504-pressure reducing valve, 505-second reversing pressure relief valve, 506-second gas storage tank, 507-second pressure switch, 508-manifold;

[0044] 600-rack. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. The components of the embodiments of the present application described and shown in the drawings here can be arranged and designed in various different configurations.

[0046] Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the present application for which protection is sought, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present application.

[0047] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0048] In the description of the embodiments of the present application, the terms "center", "up", "down", "left", "right", "vertical", "longitudinal", "lateral", "horizontal", "inside", "outside", "front", "back", "top", "bottom", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the products of the application are conventionally placed when in use, or are the orientations or positional relationships conventionally understood by those skilled in the art. They are merely for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0049] In the description of the present invention, unless otherwise clearly specified and limited, the terms "disposed", "opened", "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0050] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application may be combined with each other.

[0051] Example

[0052] Combination Figure 1 The present invention provides a time difference culture device, including a time difference culture box 500, wherein the time difference culture box 500 is adapted to be equipped with a light source assembly 400, an observation assembly and a gas supply system; the light source assembly 400 includes a first light focusing portion 401, a light decaying sheet 410 is arranged on the light incident side of the first light focusing portion 401, and the light decaying sheet 410 is sequentially provided with a plurality of light-transmitting portions 411 along the length direction of the light decaying sheet 410, and the light transmittance of the plurality of light-transmitting portions 411 increases sequentially along the length direction of the light decaying sheet 410; the observation assembly includes a microscope camera 310, a linear drive mechanism 300, the linear drive mechanism 300 is used to drive the microscope camera 310 to move along the axis of the first focusing part 401; the gas supply system includes a first pressure switch 501, a first reversing pressure relief valve 502 and a first gas storage tank 503, the first pressure switch 501 is used to monitor the air pressure in the time difference incubator 500 and control the action of the first reversing pressure relief valve 502, the first gas storage tank 503 is used to store the culture atmosphere gas of a set concentration, and the outlet of the first gas storage tank 503 is connected to the inlet of the first reversing pressure relief valve 502 after passing through the pressure reducing valve 504.

[0053] Combination Figure 2 and Figure 7 The time difference incubator 500 includes an incubator bottom plate 200, a rotating shaft 220 is rotatably connected to the middle of the incubator bottom plate 200, the rotating shaft 220 is used to drive the culture dish to rotate, the rotating shaft 220 is provided with a first sealing ring 230, the first sealing ring 230 is used to seal the gap between the rotating shaft 220 and the incubator bottom plate 200, and the first sealing ring 230 is provided with a spring clamp 240. The rotating shaft 220 is provided with an observation channel 221 along the length direction of the rotating shaft 220, so that the microscope camera lens can be inserted into the rotating shaft 220 to realize image acquisition of multiple focal planes.

[0054] Combination Figure 8 and Fig. 9 The outer side wall of the first sealing ring 230 is provided with a spring retaining groove 231 , and the spring retaining ring 240 is arranged in the spring retaining groove 231 to prevent the spring retaining ring 240 from slipping off the outer side wall of the first sealing ring 230 .

[0055] Preferably, at least two first annular protrusions 232 are arranged at intervals along the axial direction of the first sealing ring 230 on the inner wall of the first sealing ring 230, so as to seal the gap between the rotating shaft 220 and the incubator bottom plate 200 through multiple circles of annular protrusions to ensure the reliability of the seal. In order to reduce the resistance of the rotating shaft 220 to the rotation, the first annular protrusion 232 is usually arranged with only two circles. On the one hand, the first sealing ring 230 can be tightly attached to the outer wall of the rotating shaft 220 to prevent gas leakage in the incubator. On the other hand, the first sealing ring 230 body can be promptly attached to the outside of the rotating shaft 220 after the first sealing ring 230 is worn, so as to prevent the first sealing ring 230 from failing in sealing during use.

[0056] Continue to refer to Figure 8 The incubator bottom plate 200 includes a first plate body 211 and a second plate body 212 that are fixedly connected. A third sealing ring 260 is arranged between the first plate body 211 and the second plate body 212. On the one hand, it is convenient for the production and manufacturing of the incubator bottom plate 200, and on the other hand, it is convenient for the installation of the first sealing ring 230.

[0057] Furthermore, a culture dish support plate 210 is rotatably inserted in the middle of the first plate body 211, and the culture dish support plate 210 is fixedly connected to the rotating shaft 220. A groove for accommodating the bottom of the culture dish 100 is usually provided in the middle of the culture dish support plate 210 to support and fix the culture dish 100 and prevent the culture dish 100 from being relatively displaced with the rotating shaft 220 during the rotation process. As for the material of the culture dish support plate 210, a transparent material is usually used to ensure that the microscope camera 310 can normally take pictures of the biological tissue in the culture dish 100.

[0058] Furthermore, a second sealing ring 250 is disposed between the culture dish support plate 210 and the rotating shaft 220 to prevent the atmosphere in the incubator from leaking from the gap between the culture dish support plate 210 and the rotating shaft 220 .

[0059] Specifically, the rotating shaft 220 is rotatably connected to the second plate 212 .

[0060] Combination Fig.10 A plurality of second annular protrusions 261 are provided at intervals along the radial direction of the third sealing ring 260 on one end surface of the third sealing ring 260 to seal the gap between the first plate body 211 and the second plate body 212 through multiple circles of annular protrusions to ensure the reliability of the seal.

[0061] Preferably, both end surfaces of the third sealing ring 260 are provided with a plurality of second annular protrusions 261 spaced apart along the radial direction of the third sealing ring 260 , so as to further improve the sealing reliability of the third sealing ring 260 .

[0062] Continue to refer to Figure 7The time difference incubator 500 provided in this embodiment further includes a second rotation driver 270, which is in transmission connection with the rotation shaft 220 so as to drive the rotation shaft 220 to rotate.

[0063] Preferably, the output shaft of the second rotary driver 270 is connected to the rotary shaft 220 via a synchronous belt 271, so that the rotary shaft 220 and the second rotary driver 270 rotate synchronously, ensuring that the rotary shaft 220 can rotate according to a set indexing.

[0064] Combination Figure 4 The light source assembly 400 includes a first light focusing portion 401, a light decaying sheet 410 is arranged on the light incident side of the first light focusing portion 401, and the light decaying sheet 410 is sequentially arranged with a plurality of light transmitting portions 411 along the length direction of the light decaying sheet 410, and the transmittance of the plurality of light transmitting portions 411 increases sequentially along the length direction of the light decaying sheet 410.

[0065] Combination Figure 5 Specifically, the number of the light-transmitting parts 411 is three, which simplifies the structure of the light-attenuating sheet 410 while ensuring that the image taken by the microscope camera 310 has a three-dimensional and layered sense.

[0066] Preferably, the light transmittances of the three light-transmitting portions 411 are 0.8-1.2%, 12-18%, and 85-92% respectively, so that the light intensity irradiated on the biological tissue meets the imaging requirements and the light entering the first light-focusing portion 401 has a sufficient gradient. For example, the light transmittances of the three light-transmitting portions 411 are 1%, 15%, and 91% respectively.

[0067] Continue to refer to Figure 4 A second light focusing portion 402 is further provided between the first light focusing portion 401 and the light attenuation plate 410, and the volume of the second light focusing portion 402 is larger than that of the first light focusing portion 401, so as to improve the utilization rate of the light of the light source lamp 406 through two-stage focusing, and further ensure that the intensity of light irradiated on the biological tissue meets the imaging requirements.

[0068] This embodiment further includes a mounting tube 403, the first light focusing part 401 is mounted on one end of the mounting tube 403, and the light attenuation sheet 410 is mounted on the other end of the mounting tube 403 to prevent light from being scattered between the light attenuation sheet 410 and the first light focusing part 401. It should be understood that a through hole is provided in the middle of the mounting tube 403, and a mounting hole with an inner diameter increasing from the outside to the inside is provided at one end of the mounting hole 403 where the first light focusing part 401 is mounted, so as to respectively mount the first light focusing part 401 and the second light focusing part 402.

[0069] A light intake plate 404 is screwed to one end of the installation tube 403 . A step hole 405 is provided in the middle of the light intake plate 404 . The light attenuation sheet 410 is located in the step hole 405 to facilitate the installation of the light attenuation sheet 410 .

[0070] In order to facilitate the light source assembly to provide light, a light source lamp 406 is provided at the end of the step hole 405 away from the light decay plate 410. It should be understood that a cover plate 408 is fixed to the end of the light inlet plate 404 away from the mounting tube 403, and the light source lamp 406 is fixed to the middle of one end surface of the cover plate 408 facing the light inlet plate 404.

[0071] Continue to refer to Figure 4 The first light focusing portion 401 is provided with a fourth sealing ring 407 on its outer sleeve, and the fourth sealing ring 407 is used to seal the gap between the first light focusing portion 401 and the mounting tube 403 to prevent the gas in the incubator where the culture dish 100 is placed from leaking through the mounting tube 403.

[0072] Combination Figure 6 The linear drive mechanism 300 includes a lead screw 301, a slider 302 and a slide rail 303, and the microscopic camera 310 is fixed on the slider 302. It can be understood that it also includes a first rotary driver 308, the output shaft of which is fixedly connected to the lead screw 301, and the microscopic camera 310 is driven to move along the axis of the rotation shaft 220 through the lead screw 301 and the slider 302 mechanism. On the one hand, the position of the microscopic camera 310 can be maintained through the self-locking of the lead screw 301 and the slider 302 mechanism, and on the other hand, the movement accuracy of the microscopic camera 310 can be ensured.

[0073] Furthermore, a driving block 304 and a supporting block 305 are fixed at intervals on the slider 302, and the driving block 304 is threadedly connected to the lead screw 301 through an anti-backlash nut 306, and an anti-backlash spring 307 is arranged between the anti-backlash nut 306 and the supporting block 305. The microscope camera 310 is fixed on the supporting block 305 so that the thread of the anti-backlash nut 306 is tightly attached to the thread of the lead screw 301, thereby preventing transmission gap from existing during the transmission process of the lead screw, and realizing that the microscope camera 310 does not lose steps under the control of a conventional stepping motor, thereby ensuring that the microscope camera 310 moves according to the set distance, thereby ensuring the clarity of the shooting.

[0074] Preferably, a spring fixing groove 309 is provided at one end of the support block 305 facing the anti-backlash nut 306 , and one end of the anti-backlash spring 307 is inserted into the spring fixing groove 309 to prevent the anti-backlash spring 307 from slipping off.

[0075] Combination Fig.11The gas supply system includes a first pressure switch 501, a first reversing pressure relief valve 502 and a first gas storage tank 503. The first pressure switch 501 is used to monitor the air pressure in the time difference incubator 500 and control the operation of the first reversing pressure relief valve 502. The first gas storage tank 503 is used to store the culture atmosphere gas of a set concentration. The outlet of the first gas storage tank 503 is connected to the inlet of the first reversing pressure relief valve 502 after passing through a pressure reducing valve 504.

[0076] In this embodiment, the first reversing pressure relief valve 502 is a two-position three-way valve to prevent the gas output from the first gas storage tank 503 from being discharged to the outside through the first reversing pressure relief valve 502 when the time difference incubator 500 is depressurized, thereby causing waste of culture atmosphere gas. It can be understood that the first reversing pressure relief valve 502 only needs to be able to control the conduction of the gas input into the time difference incubator 500 or to connect the time difference incubator 500 to the outside, and can be a two-position four-way valve, a two-position five-way valve, etc., or the first reversing pressure relief valve 502 can be split into two parallel switch valves.

[0077] Furthermore, a second reversing pressure relief valve 505 is connected between the first gas storage tank 503 and the pressure reducing valve 504 , so as to control the maximum output pressure of the first gas storage tank 503 through the second reversing pressure relief valve 505 .

[0078] Similarly, in this embodiment, the second reversing pressure relief valve 505 is a two-position three-way valve to prevent the gas output from the first gas tank 503 from being exhausted to the outside through the second reversing pressure relief valve 505 when the pressure is released at the front end of the pressure reducing valve 504, thereby causing waste of the culture atmosphere gas.

[0079] Furthermore, a second gas storage tank 506 is connected between the pressure reducing valve 504 and the first reversing pressure relief valve 502 to provide a buffer for the gas flowing out of the outlet end of the pressure reducing valve 504 through the second gas storage tank 506, so as to stabilize the intake pressure of the intake end of the first reversing pressure relief valve 502 and reduce the operation frequency of the first reversing pressure relief valve 502, thereby saving the culture atmosphere gas.

[0080] Furthermore, the second gas storage tank 506 is connected to a second pressure switch 507, and the second pressure switch 507 is used to monitor the pressure of the second gas storage tank 506 and control the action of the second reversing pressure relief valve 505 to prevent the gas storage pressure of the second gas storage tank 506 from being too high.

[0081] Preferably, the first pressure switch 501 and the first reversing pressure relief valve 502 are correspondingly arranged in multiple groups to provide culture atmosphere gas for multiple time difference incubators 500 at the same time.

[0082] Preferably, the plurality of first reversing pressure relief valves 502 are connected to the second gas storage tank 506 via a manifold 508 to simplify the pipeline of the supply system.

[0083] When in use, the first pressure switch 501 and the first reversing pressure relief valve 502 are respectively connected to the time difference incubator 500, the culture atmosphere gas of a set concentration is pressurized in the first gas tank 503, and the culture dish 100 is placed in the groove of the culture dish support plate 210, and the first gas tank 503 and the regulating pressure reducing valve 504 are connected through the second reversing pressure relief valve 505.

[0084] Then, the pressure reducing valve 504 is adjusted so that the gas in the first gas tank 503 is input into the second gas tank 506 at the set pressure, and the gas pressure of the second gas tank 506 is monitored in real time through the second pressure switch 507. When the pressure in the second gas tank 506 is higher than the set value input into the first reversing pressure relief valve 502, the second pressure switch 507 outputs an action signal to the second reversing pressure relief valve 505 (supplying power to the second reversing pressure relief valve 505), and the second reversing pressure relief valve 505 disconnects the first gas tank 503 and the regulating pressure reducing valve 504, connects the second gas tank 506 to the external environment, and closes the first gas tank 503, thereby controlling the gas pressure of the second gas tank 506 within the set range.

[0085] The pressure-regulated gas is stored in the second gas storage tank 506 and input into the time difference incubator 500 through the first reversing pressure relief valve 502. The gas pressure in the time difference incubator 500 is monitored in real time by the first pressure switch 501. When the gas pressure in the time difference incubator 500 is higher than the set upper limit value, the first pressure switch 501 controls the first reversing pressure relief valve 502 to reverse (supply power to the first reversing pressure relief valve 502), thereby disconnecting the second gas storage tank 506 and the time difference incubator 500, connecting the time difference incubator 500 with the external environment, and closing the second gas storage tank 506 to relieve the pressure of the time difference incubator 500. When the gas pressure in the time difference incubator 500 is lower than the set lower limit value, the first pressure switch 501 controls the first reversing pressure relief valve 502 to reset (disconnect the power supply of the first reversing pressure relief valve 502), so as to input the culture atmosphere gas into the time difference incubator 500, so that the gas pressure in the time difference incubator 500 is maintained at the set value.

[0086] When observation is required, the light of the light source lamp 406 is attenuated by the light attenuation sheet 410 to form a gradient light, and then the light is sequentially focused on the axis of the microscope camera 310 through the second focusing part 402 and the first focusing part 401. The culture dish support plate 210 is driven to rotate by the rotating shaft 220, thereby driving the culture dish 100 to rotate around its own axis, so that the culture dish 100 can be rotated relative to the microscope camera 310 in the time difference incubator to scan the circumferentially distributed biological tissue. The culture dish support plate 210 is driven to rotate by the rotating shaft 220, thereby driving the culture dish 100 to rotate, so that the culture dish 100 can be rotated relative to the optical component and the microscope camera 310 in the time difference incubator to scan the circumferentially distributed biological tissue.

[0087] A light attenuation sheet 410 is provided on the light incident side of the first focusing portion 401, which can attenuate the light emitted by the light source lamp 406 and reduce the light damage of the light source to the biological tissue. A plurality of light-transmitting portions 411 with successively increasing light transmittances are sequentially arranged on the light attenuation sheet 410 along the length direction of the light attenuation sheet 410, which can form the light emitted by the light source lamp 406 into gradient light and then focus it to the biological tissue observation area of ​​the culture dish 100 through the first focusing portion 401, so that the biological tissue image with three-dimensional and layered sense can be captured by the microscope camera 310, so as to accurately judge the morphology of the biological tissue and ensure the accuracy of the evaluation, analysis and screening of the biological tissue.

[0088] Among them, a plurality of light-transmitting portions 411 with increasing light transmittance are sequentially arranged on the light-decaying sheet 410 along the length direction of the light-decaying sheet 410, which can not only form light with various light intensities, but also make the light intensity irradiated on the biological tissue meet the imaging requirements. The second gas storage tank 506 provides a buffer for the gas flowing out of the outlet of the pressure reducing valve 504 to stabilize the intake pressure of the intake end of the first reversing pressure relief valve 502, reduce the operation frequency of the first reversing pressure relief valve 502, and thus save the culture atmosphere gas; the gas stored in the first gas storage tank 503 is the culture atmosphere gas of the set concentration, and there is no need to adjust and monitor the gas concentration in the time difference incubator 500, which can simplify the gas supply of the time difference incubator 500.

[0089] In addition, a first sealing ring 230 is provided on the outer sleeve of the rotating shaft 220, and a spring clamp ring 240 is provided on the outer sleeve of the first sealing ring 230. On the one hand, the first sealing ring 230 can be tightly attached to the outer wall of the rotating shaft 220 to prevent gas leakage in the incubator. On the other hand, after the first sealing ring 230 is worn, the first sealing ring 230 body can be promptly attached to the outside of the rotating shaft 220 to prevent the first sealing ring 230 from failing in sealing during use.

[0090] Therefore, this embodiment can avoid repeated focusing of the microscope photography system, ensure the clarity of the captured image, and at the same time, capture biological tissue images with three-dimensional and layered effects through the microscope camera 310 to accurately determine the morphology of the biological tissue and ensure the accuracy of the biological tissue evaluation, analysis and screening.

[0091] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. According to the technical essence of the present invention, within the spirit and principles of the present invention, any simple modification, equivalent replacement and improvement made to the above embodiment still falls within the protection scope of the technical solution of the present invention.

Claims

1. A time difference culture device, comprising a time difference culture box (500), characterized in that: The time difference incubator (500) is adapted to be equipped with a light source assembly (400), an observation assembly and a gas supply system; The light source assembly (400) comprises a first light focusing portion (401), a light decaying sheet (410) being arranged on the light incident side of the first light focusing portion (401), the light decaying sheet (410) being provided with a plurality of light-transmitting portions (411) in sequence along the length direction of the light decaying sheet (410), and light transmittances of the plurality of light-transmitting portions (411) increasing in sequence along the length direction of the light decaying sheet (410); The observation assembly comprises a microscope camera (310) and a linear drive mechanism (300), wherein the linear drive mechanism (300) is used to drive the microscope camera (310) to move along the axis of the first light focusing portion (401); The gas supply system comprises a first pressure switch (501), a first reversing pressure relief valve (502) and a first gas storage tank (503), wherein the first pressure switch (501) is used to monitor the gas pressure in the time difference incubator (500) and control the operation of the first reversing pressure relief valve (502), and the first gas storage tank (503) is used to store a culture atmosphere gas of a set concentration, and the outlet of the first gas storage tank (503) is connected to the inlet of the first reversing pressure relief valve (502) after passing through a pressure reducing valve (504); A second light focusing portion (402) is further provided between the first light focusing portion (401) and the light attenuation plate (410), and the volume of the second light focusing portion (402) is greater than the volume of the first light focusing portion (401); The incubator bottom plate (200) of the time-difference incubator (500) is rotatably connected to a rotating shaft (220), the upper end of the rotating shaft (220) is used to support the culture dish (100), and the rotating shaft (220) can rotate around its own axis; an observation channel (221) is provided in the middle of the rotating shaft (220), and the microscope camera (310) moves in the observation channel (221) along the axis of the first focusing portion (401).

2. The time difference culture device according to claim 1, characterized in that: The outer wall of the rotating shaft (220) is provided with a first sealing ring (230), the first sealing ring (230) being used to seal the gap between the rotating shaft (220) and the bottom plate (200) of the incubator, and the outer wall of the first sealing ring (230) is provided with a spring clamp ring (240).

3. The time difference culture device according to claim 1, characterized in that: The linear drive mechanism (300) comprises a lead screw (301), a slider (302) and a slide rail (303), and the microscopic camera (310) is fixed on the slider (302).

4. The time difference culture device according to claim 3, characterized in that: A driving block (304) and a supporting block (305) are fixed on the slider (302) at intervals; the driving block (304) is threadedly connected to the lead screw (301) via an anti-backlash nut (306); an anti-backlash spring (307) is provided between the anti-backlash nut (306) and the supporting block (305); and the microscope camera (310) is fixed on the supporting block (305).

5. The time difference culture device according to claim 1, characterized in that: A second reversing pressure relief valve (505) is connected between the first gas storage tank (503) and the pressure reducing valve (504).

6. The time difference culture device according to claim 5, characterized in that: A second gas storage tank (506) is connected between the pressure reducing valve (504) and the first reversing pressure relief valve (502).

7. The time difference culture device according to claim 6, characterized in that: The second gas storage tank (506) is connected to a second pressure switch (507), and the second pressure switch (507) is used to monitor the pressure of the second gas storage tank (506) and control the operation of the second reversing pressure relief valve (505).

8. The time difference culture device according to any one of claims 1 to 7, characterized in that: There are a plurality of time difference incubators (500) in an array, and the plurality of time difference incubators (500) are fixed on a rack (600).

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