A cell storage device with heat preservation and refrigeration function and a method of using the same

Abstract

The present invention belongs to the field of cell storage equipment, and in particular, a cell storage device with heat preservation and refrigeration functions and a method of using the same, wherein the cell storage device with heat preservation and refrigeration functions comprises a shockproof bottom plate, two side plates are fixedly installed on the top of the shockproof bottom plate, and the same storage box is slidably installed on the side where the two side plates are close to each other, and a heat preservation plate is fixedly installed in the storage box, and a semiconductor refrigeration plate is fixedly installed on the bottom inner wall of the storage box, and fans are fixedly connected on both sides of the semiconductor refrigeration plate, and two fixing frames are fixedly installed on the bottom inner wall of the storage box, and fixing plates are provided on both fixing frames. The present invention has a reasonable structure and is easy to operate. The internal structure and functions of the cell storage device are rich, and it has refrigeration and heat preservation functions. It can store cells with special storage requirements, and the storage device has good stability and buffering properties, and can provide better protection for culture bottles.

Description

Technical Field

[0001] The present invention relates to the technical field of cell storage equipment, and in particular to a cell storage device with heat preservation and refrigeration functions and a use method thereof. Background Art

[0002] Stem cell transplantation for the treatment of many difficult diseases such as heart disease, nervous system diseases, blood diseases, etc. has become a hot topic in stem cell research and application. It is also a method for clinical treatment of difficult diseases. Stem cell research and clinical application is a systematic work coordinated by multiple disciplines, multiple units, and multiple departments. At present, stem cell culture technology has become increasingly mature, and the application of stem cells has also been carried out in depth. Stem cell clinical research and treatment require independent cell preparation units to provide cells. To provide cells to cell-using units, so that cells can achieve optimal vitality in both research and development and clinical application, a standardized cell storage and transportation process that can meet standards is required;

[0003] However, the existing cell storage devices have a single internal structure and function, do not have refrigeration and heat preservation functions, cannot store cells with special storage requirements, and have poor stability and buffering properties, and cannot provide good protection for cells. In addition, the suitable storage temperature difference between stem cells and other common biological cells is large. Therefore, when storing different types of samples, it is necessary to switch the refrigeration equipment, which makes the control of the entire device more complicated. In addition, it is impossible to achieve refrigeration of different types of samples. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a cell storage device with heat preservation and refrigeration functions and a method of using the same.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A cell storage device with heat preservation and refrigeration functions comprises an anti-seismic bottom plate, two side plates are fixedly installed on the top of the anti-seismic bottom plate, a storage box is slidably installed on the side where the two side plates are close to each other, a heat preservation plate is fixedly installed in the storage box, a semiconductor refrigeration plate is fixedly installed on the bottom inner wall of the storage box, fans are fixedly connected to both sides of the semiconductor refrigeration plate, two fixing frames are fixedly installed on the bottom inner wall of the storage box, both fixing frames are provided with fixing plates, fixing grooves are opened at the bottom of the two fixing plates, the fixing frames are slidably connected in the corresponding fixing grooves, a cover plate is fixedly installed on the top of the two fixing plates, a handle is fixedly installed on the top of the cover plate, a plurality of placement plates are fixedly installed on the side where the two fixing plates are close to each other, a plurality of culture bottles are placed on the top of the placement plate, two sliding holes are opened on the top of the placement plate, sliding rods are slidably installed in the two sliding holes, and two A bottom plate is fixedly installed at the bottom end of the sliding rod, and a first spring is sleeved on the two sliding rods. The top of the two sliding rods is fixedly installed with the same stabilizing plate, and the bottom of the stabilizing plate contacts with multiple culture bottles. The bottom of the stabilizing plate is provided with multiple connecting grooves, and clamping plates are slidably installed in two of the multiple connecting grooves, and the two clamping plates contact the two sides of the culture bottles. One side of the two clamping plates is provided with a threaded hole, and the same through hole is provided on the inner wall of the side where the two connecting grooves are close to each other, and two screw rods are rotatably installed in the through hole, and the screw rods are rotatably connected in the corresponding threaded holes. A rotating wheel is fixedly installed on the ends of the two screw rods close to each other, and the two rotating wheels are meshed with each other. A first gear is fixedly sleeved on the screw rod, and a connecting hole is provided on the top inner wall of the connecting groove, and a first rack is slidably installed in the connecting hole, and the first rack is fixedly connected to the top of the placement plate, and the first rack is meshed with the first gear.

[0007] Preferably, two installation grooves are provided on the top of the seismic-resistant base plate, buffer arms are rotatably installed in the two installation grooves, rollers are rotatably installed on the two buffer arms, both rollers are in contact with the bottom of the storage box, a first bevel gear is fixedly installed on one side of the two buffer arms, a rotating groove is provided on the inner wall of one side of the two installation grooves, and fixed rods are rotatably installed in the two rotating grooves.

[0008] Preferably, a second bevel gear is fixedly installed at one end of the two fixed rods, the second bevel gear is meshed with the corresponding first bevel gear, a winding wheel is fixedly sleeved on the two fixed rods, a pull rope is wound around the two winding wheels, two second springs are fixedly installed on the top of the seismic-resistant base plate, anti-slip plates are fixedly installed on the top of the two second springs, and the top of the pull rope is fixedly connected to the bottom of the corresponding anti-slip plate.

[0009] Preferably, an auxiliary groove is provided on the inner wall of the installation groove, and an auxiliary block is fixedly installed on one side of the buffer arm, and the auxiliary block is rotatably connected in the auxiliary groove.

[0010] Preferably, two limit slots are provided on one side of the two fixing frames, grooves are provided on the inner walls of one side of the two fixing slots, limit blocks are slidably installed in the two grooves, and the limit blocks are installed in the corresponding limit slots.

[0011] Preferably, a second rack is fixedly installed on one side of the two limit blocks, a fixing cavity is opened on the cover plate, two adjustment holes are opened on the bottom inner wall of the fixing cavity, adjusting rods are rotatably installed in the two adjustment holes, and second gears are fixedly installed on the bottom ends of the two adjusting rods, and the second gears are meshed with the corresponding second racks.

[0012] Preferably, a third bevel gear is fixedly installed on the top of the two adjusting rods, two rotating rods are rotatably installed in the fixed cavity, a fourth bevel gear is fixedly installed on the ends of the two rotating rods away from each other, the fourth bevel gear is meshed with the corresponding third bevel gear, and the same worm gear is fixedly installed on the ends of the two rotating rods close to each other, a round rod is rotatably installed on the top inner wall of the fixed cavity, a worm is fixedly installed on the bottom end of the round rod, the worm is meshed with the worm wheel, and a switch dial is fixedly installed on the top of the round rod.

[0013] The present invention also provides a method for using a cell storage device with heat preservation and refrigeration functions, comprising the following steps:

[0014] S1: Pull the stabilizing plate, the stabilizing plate drives the sliding rod to move, the sliding rod drives the bottom plate to move and squeeze the first spring, the first spring undergoes elastic deformation, multiple culture bottles are placed on the placement plate, the stabilizing plate is released, the elastic action of the first spring can drive the stabilizing plate to reset and move, the stabilizing plate clamps and fixes the multiple culture bottles, and at the same time the first gear moves and rotates on the first rack, so that the first gear drives the screw rod to rotate, the screw rod drives another screw rod to rotate through the rotating wheel, so that the screw rod drives the corresponding clamping plate to move, the two clamping plates can stabilize the two sides of the culture bottle, and can better limit the position of the culture bottle;

[0015] S2: When it is necessary to cold store the stem cell samples at a lower temperature, the cover plate is moved downward to the bottom, and the cover plate drives the two fixed plates to move, and the fixed plates slide on the corresponding fixed frames, and the fixed plates drive the placement plates and multiple culture bottles into the storage box, and the switch dial is turned, and the switch dial drives the round rod to rotate, and the round rod drives the worm to rotate, and the worm controls the worm gear to rotate, and the worm gear can drive the two rotating rods to rotate, and the rotating rod controls the fourth bevel gear to rotate, and the fourth bevel gear drives the corresponding adjustment rod on the third bevel gear to rotate, and the adjustment rod can drive the corresponding second gear to rotate, and the second gear drives the corresponding limit block to move, and the limit block slides into the corresponding limit groove, which can fix the position of the cover plate, and the insulation plate can improve the insulation effect in the storage box. In the process of moving the cover plate to drive the fixed plate to descend, the volume of the first cavity in the fixed plate is compressed, so that the liquid nitrogen preparation is squeezed into the second cavity of each placement plate, and overflows from each sample mounting hole to contact the culture bottle thereon, thereby realizing low temperature storage;

[0016] If other samples with a higher suitable temperature need to be stored, the movable cover plate is moved to the top. Since the placement plate body is designed to be high in the middle and low on both sides, the liquid nitrogen preparation automatically flows back to the first cavity. If multiple samples with some lower temperatures and some higher temperatures need to be stored, the cover plate is moved downward to the middle position of the maximum descending stroke, and the stem cells that need a lower temperature are placed on the placement plate at the bottom, and other types of samples are placed on the placement plates at the higher layers, so that the liquid nitrogen preparation can only enter the second cavity in the placement plates at the lower layers. In this process, the semiconductor refrigeration sheet cools the storage box, and cooperates with two fans to drive the air flow in the storage box, so that the refrigeration speed is faster.

[0017] S3: The storage box can provide better protection for the culture bottles, and when the storage box shakes during transportation, the storage box will squeeze the rollers on the two buffer arms. After being squeezed, the buffer arm drives the first bevel gear to rotate, the first bevel gear drives the second bevel gear to rotate, the second bevel gear drives the fixed rod to rotate, the fixed rod drives the winding wheel to rotate, the winding wheel can reel in the pull rope, so that the pull rope pulls the anti-slip sheet, the anti-slip sheet squeezes the second spring, the elastic action of the second spring can drive the anti-slip sheet to reset, and provide a better buffering effect for the buffer arm, thereby playing a shock-absorbing and buffering role for the storage box.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] 1. The present invention has a reasonable structure and is easy to operate. The internal structure and functions of the cell storage device are rich, and it has refrigeration and heat preservation functions. It can store cells with special storage requirements, and the storage device has good stability and buffering properties, which can provide better protection for culture bottles. 2. In the present invention, the placement plate is loaded through the specially designed first cavity and the second cavity and the specially shaped mounting hole, so that different types of samples can be stored and multiple different samples can be refrigerated at the same time only by moving up and down. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a schematic diagram of the main structure proposed by the present invention;

[0021] Figure 2 This is a schematic diagram of the structure of part A proposed by the present invention;

[0022] Figure 3 This is a schematic diagram of the structure of part B proposed by the present invention;

[0023] Figure 4 This is a schematic diagram of the structure of part C proposed by the present invention;

[0024] Figure 5 This is a schematic structural diagram of the D part proposed by the present invention.

[0025] In the figure: 1. earthquake-resistant bottom plate; 2. side plate; 3. storage box; 4. fixing frame; 5. fixing plate; 6. cover plate; 7. handle; 8. semiconductor cooling plate; 9. fan; 10. placement plate; 11. slide rod; 12. first spring; 13. stabilizing plate; 14. clamping plate; 15. screw rod; 16. rotating wheel; 17. first gear; 18. first rack; 19. culture bottle; 20. insulation plate; 21. limit groove; 22. limit block; 23. second gear; 24. adjusting rod; 25. rotating rod; 26. worm gear; 27. worm; 28. switch dial; 29. ​​buffer arm; 30. roller; 31. first bevel gear; 32. fixing rod; 33. second bevel gear; 34. winding wheel; 35. pull rope; 36. anti-drop sheet; 37. second spring. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0027] Unless otherwise defined, the technical or scientific terms used herein shall have the common meanings understood by persons with ordinary skills in the field to which the present invention belongs. The words "first", "second" and similar words used in the patent specification and claims of the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, words such as "one" or "an" do not indicate quantity restrictions, but indicate the existence of at least one.

[0028] Reference Figure 1-5A cell storage device with heat preservation and refrigeration function comprises an anti-seismic bottom plate 1, two side plates 2 are fixedly installed on the top of the anti-seismic bottom plate 1, a storage box 3 is slidably installed on the side of the two side plates 2 close to each other, a heat preservation plate 20 is fixedly installed in the storage box 3, a semiconductor refrigeration sheet 8 is fixedly installed on the bottom inner wall of the storage box 3, fans 9 are fixedly connected to both sides of the semiconductor refrigeration sheet 8, two fixing frames 4 are fixedly installed on the bottom inner wall of the storage box 3, a first cavity is formed between the fixing frame 4 and the fixing groove, a liquid nitrogen preparation is pre-stored in the first cavity, and a fixing sleeve is slidably provided on the two fixing frames 4 Plate 5, a U-shaped fixing groove is provided at the bottom of the two fixing plates 5, and the fixing frame 4 is slidably connected in the corresponding fixing groove. A sealing gasket is covered on the end of the fixing frame 4 body facing the fixing groove. The upper half of the sealing gasket is roughened and the lower half is polished. The same cover plate 6 is fixedly installed on the top of the two fixing plates 5, and a handle 7 is fixedly installed on the top of the cover plate 6. A plurality of placement plates 10 are fixedly installed on the side where the two fixing plates 5 are close to each other. The placement plate 10 is designed as a whole with a high middle and low sides. A second cavity is provided in the placement plate 10 body, and the second cavity is connected to the first cavity. The placement plate 10 The top of the placing plate 10 is provided with a plurality of sample mounting holes, the mounting holes are communicated with the second cavity and a plurality of culture bottles 19 are placed in the mounting holes, the top of the placing plate 10 is provided with two sliding holes, a sliding rod 11 is slidably mounted in each of the two sliding holes, a bottom plate is fixedly mounted at the bottom ends of the two sliding rods 11, a first spring 12 is sleeved on each of the two sliding rods 11, a same stabilizing plate 13 is fixedly mounted at the top ends of the two sliding rods 11, the bottom of the stabilizing plate 13 is in contact with the plurality of culture bottles 19, a plurality of connecting grooves are provided at the bottom of the stabilizing plate 13, a clamping plate 14 is slidably mounted in two of the connecting grooves of the plurality of connecting grooves, the two clamping plates 14 and The two sides of the culture bottle 19 are in contact with each other, and a threaded hole is provided on one side of the two clamping plates 14. The same through hole is provided on the inner wall of the side where the two connecting grooves are close to each other. Two screw rods 15 are rotatably installed in the through hole. The screw rods 15 are rotatably connected in the corresponding threaded holes. A rotating wheel 16 is fixedly installed on the ends of the two screw rods 15 close to each other. The two rotating wheels 16 are meshed with each other. A first gear 17 is fixedly sleeved on the screw rod 15. A connecting hole is provided on the top inner wall of the connecting groove. A first rack 18 is slidably installed in the connecting hole. The first rack 18 is fixedly connected to the top of the placement plate 10, and the first rack 18 is meshed with the first gear 17.

[0029] In this embodiment, two installation grooves are provided on the top of the seismic-resistant base plate 1, and buffer arms 29 are rotatably installed in the two installation grooves. Rollers 30 are rotatably installed on the two buffer arms 29, and the two rollers 30 are in contact with the bottom of the storage box 3. A first bevel gear 31 is fixedly installed on one side of the two buffer arms 29, and a rotating groove is provided on the inner wall of one side of the two installation grooves, and a fixed rod 32 is rotatably installed in the two rotating grooves.

[0030] In this embodiment, a second bevel gear 33 is fixedly installed at one end of the two fixed rods 32, and the second bevel gear 33 is meshed with the corresponding first bevel gear 31. A winding wheel 34 is fixedly sleeved on the two fixed rods 32, and a pull rope 35 is wound around the two winding wheels 34. Two second springs 37 are fixedly installed on the top of the seismic-resistant base plate 1, and anti-slip plates 36 are fixedly installed on the top ends of the two second springs 37. The top ends of the pull ropes 35 are fixedly connected to the bottom ends of the corresponding anti-slip plates 36.

[0031] In this embodiment, an auxiliary groove is opened on the inner wall of the installation groove, and an auxiliary block is fixedly installed on one side of the buffer arm 29. The auxiliary block is rotatably connected in the auxiliary groove. When the buffer arm 29 rotates, the auxiliary block is driven to rotate in the auxiliary groove, which can improve the stability of the buffer arm 29 when rotating.

[0032] In this embodiment, two limit slots 21 are provided on one side of the two fixing frames 4, and grooves are provided on the inner walls of one side of the two fixing slots. Limit blocks 22 are slidably installed in the two grooves, and the limit blocks 22 are installed in the corresponding limit slots 21.

[0033] In this embodiment, a second rack is fixedly installed on one side of the two limit blocks 22, a fixing cavity is opened on the cover plate 6, and two adjustment holes are opened on the bottom inner wall of the fixing cavity. Adjustment rods 24 are rotatably installed in the two adjustment holes, and second gears 23 are fixedly installed on the bottom ends of the two adjustment rods 24, and the second gears 23 are meshed with the corresponding second racks.

[0034] In this embodiment, a third bevel gear is fixedly installed on the top of the two adjusting rods 24, and two rotating rods 25 are rotatably installed in the fixed cavity. A fourth bevel gear is fixedly installed on the ends of the two rotating rods 25 that are away from each other, and the fourth bevel gear is meshed with the corresponding third bevel gear. The ends of the two rotating rods 25 that are close to each other are fixedly installed with the same worm gear 26, and a round rod is rotatably installed on the top inner wall of the fixed cavity, and a worm 27 is fixedly installed on the bottom end of the round rod, which is meshed with the worm gear 26, and a switch dial 28 is fixedly installed on the top of the round rod.

[0035] This embodiment also provides a method for using a cell storage device with heat preservation and refrigeration functions, comprising the following steps:

[0036] S1: Pull the stabilizing plate 13, the stabilizing plate 13 drives the sliding rod 11 to move, the sliding rod 11 drives the bottom plate to move and squeeze the first spring 12, the first spring 12 is elastically deformed, and multiple culture bottles 19 are placed on the placement plate 10, and the stabilizing plate 13 is released. The elastic action of the first spring 12 can drive the stabilizing plate 13 to reset and move, and the stabilizing plate 13 clamps and fixes the multiple culture bottles 19. At the same time, the first gear 17 moves and rotates on the first rack 18, so that the first gear 17 drives the screw rod 15 to rotate, and the screw rod 15 drives another screw rod 15 to rotate through the rotating wheel 16, so that the screw rod 15 drives the corresponding clamping plate 14 to move, and the two clamping plates 14 can stabilize the two sides of the culture bottle 19, and can better limit the position of the culture bottle 19;

[0037] S2: When it is necessary to cold store the stem cell samples at a lower temperature, the cover plate 6 is moved downward to the bottom, the cover plate 6 drives the two fixed plates 5 to move, the fixed plates 5 slide on the corresponding fixed frames 4, the fixed plates 5 drive the placement plates 10 and multiple culture bottles 19 to enter the storage box 3, the switch dial 28 is turned, the switch dial 28 drives the round rod to rotate, the round rod drives the worm 27 to rotate, the worm 27 controls the worm gear 26 to rotate, the worm gear 26 can drive the two rotating rods 25 to rotate, the rotating rod 25 controls the fourth bevel gear to rotate, the fourth bevel gear drives the corresponding third bevel gear The adjusting rod 24 rotates, and the adjusting rod 24 can drive the corresponding second gear 23 to rotate. The second gear 23 drives the corresponding limit block 22 to move. The limit block 22 slides into the corresponding limit groove 21, which can fix the position of the cover plate 6. The insulation plate 20 can improve the insulation effect in the storage box 3. In the process of the movable cover plate 6 driving the fixed plate 5 to descend, the volume of the first cavity in the fixed plate 5 is compressed, so that the liquid nitrogen preparation is squeezed into the second cavity of each placement plate 10, and overflows from each sample installation hole to contact the culture bottle 19 thereon, thereby realizing low-temperature storage;

[0038] If it is necessary to store other samples at a higher suitable temperature, the movable cover plate 6 is moved to the top. Since the placement plate 10 is designed to be high in the middle and low on both sides, the liquid nitrogen preparation automatically flows back to the first cavity. If it is necessary to store multiple samples with some lower temperatures and some higher temperatures, the cover plate 6 is moved downward to the middle position of the maximum descending stroke, and the stem cells that need a lower temperature are placed on the bottom placement plate 10, and other types of samples are placed on the placement plates 10 on the higher layers, so that the liquid nitrogen preparation can only enter the second cavity in the placement plates 10 on the lower layers. In this process, the semiconductor refrigeration sheet 8 cools the storage box 3, and cooperates with the two fans 9 to drive the air flow in the storage box 3, so that the cooling speed is faster.

[0039] S3: The storage box 3 can provide better protection for the culture bottle 19, and when the storage box 3 shakes during transportation, the storage box 3 will squeeze the rollers 30 on the two buffer arms 29. After being squeezed, the buffer arms 29 drive the first bevel gear 31 to rotate, the first bevel gear 31 drives the second bevel gear 33 to rotate, the second bevel gear 33 drives the fixed rod 32 to rotate, the fixed rod 32 drives the winding wheel 34 to rotate, the winding wheel 34 can reel in the pull rope 35, so that the pull rope 35 pulls the anti-slip sheet 36, and the anti-slip sheet 36 squeezes the second spring 37. The elastic action of the second spring 37 can drive the anti-slip sheet 36 to reset and provide a better buffering effect for the buffer arm 29, thereby playing a shock-absorbing and buffering role for the storage box 3.

[0040] Therefore, in the present invention, the placement plate is loaded through the specially designed first cavity and the second cavity and the specially designed mounting hole, so that different types of samples can be stored and multiple different samples can be refrigerated at the same time only by moving up and down.

[0041] The technical progress achieved by the present invention compared with the prior art is: the present invention has a reasonable structure and is easy to operate. The internal structure and functions of the cell storage device are rich, and it has refrigeration and heat preservation functions. It can store cells with special storage requirements, and the storage device has good stability and buffering properties, which can provide better protection for the culture bottle 19.

Claims

1. A cell storage device with heat preservation and refrigeration functions, comprising a shock-resistant bottom plate (1), characterized in that: Two side plates (2) are fixedly mounted on the top of the earthquake-resistant bottom plate (1), and a storage box (3) is slidably mounted on the side of the two side plates (2) close to each other. A heat preservation plate (20) is fixedly mounted inside the storage box (3). A semiconductor refrigeration plate (8) is fixedly mounted on the inner wall of the bottom of the storage box (3), and fans (9) are fixedly connected to both sides of the semiconductor refrigeration plate (8). Two fixing frames (4) are fixedly mounted on the inner wall of the bottom of the storage box (3), and a first cavity is formed between the frame body of the fixing frame (4) and the fixing groove, and liquid nitrogen preparation is pre-stored in the first cavity. A fixing plate (5) is slidably mounted on each of the two fixing frames (4), and the bottoms of the two fixing plates (5) are provided with There is a U-shaped fixing groove, the fixing frame (4) is slidably connected in the corresponding fixing groove, a sealing gasket is covered on the end of the fixing frame (4) facing the fixing groove, the upper half of the sealing gasket is roughened, and the lower half is polished, the top of the two fixing plates (5) is fixedly installed with the same cover plate (6), the top of the cover plate (6) is fixedly installed with a handle (7), and a plurality of placement plates (10) are fixedly installed on the side where the two fixing plates (5) are close to each other, and the placement plate (10) is designed as a whole with a high middle and low sides, and a second cavity is opened in the plate body of the placement plate (10), and the second cavity is connected to the first cavity, and a plurality of sample installation holes are opened on the top of the placement plate (10) The mounting hole is connected to the second cavity and a plurality of culture bottles (19) are placed in the mounting hole. Two sliding holes are provided at the top of the placement plate (10). Sliding rods (11) are slidably installed in the two sliding holes. A bottom plate is fixedly installed at the bottom ends of the two sliding rods (11). The two sliding rods (11) are sleeved with a first spring (12). The top ends of the two sliding rods (11) are fixedly installed with a same stabilizing plate (13). The bottom of the stabilizing plate (13) is in contact with the plurality of culture bottles (19). The bottom of the stabilizing plate (13) is provided with a plurality of connecting grooves. A clamping plate (14) is slidably installed in two of the connecting grooves. The two clamping plates (14) are fixedly installed with the culture bottles (19). The two sides of the two clamping plates (14) are in contact with each other, a threaded hole is provided on one side of the two clamping plates (14), a same through hole is provided on the inner wall of the side where the two connecting grooves are close to each other, two screw rods (15) are rotatably installed in the through hole, the screw rods (15) are rotatably connected in the corresponding threaded holes, a rotating wheel (16) is fixedly installed on the ends of the two screw rods (15) close to each other, the two rotating wheels (16) are meshed, a first gear (17) is fixedly sleeved on the screw rods (15), a connecting hole is provided on the top inner wall of the connecting groove, a first rack (18) is slidably installed in the connecting hole, the first rack (18) is fixedly connected to the top of the placement plate (10), and the first rack (18) is meshed with the first gear (17).

2. A cell storage device with heat preservation and refrigeration function according to claim 1, characterized in that: The top of the anti-seismic bottom plate (1) is provided with two installation grooves, buffer arms (29) are rotatably installed in the two installation grooves, rollers (30) are rotatably installed on the two buffer arms (29), and the two rollers (30) are in contact with the bottom of the storage box (3), a first bevel gear (31) is fixedly installed on one side of the two buffer arms (29), a rotating groove is provided on the inner wall of one side of the two installation grooves, and a fixing rod (32) is rotatably installed in the two rotating grooves.

3. A cell storage device with heat preservation and refrigeration function according to claim 2, characterized in that: A second bevel gear (33) is fixedly mounted on one end of the two fixed rods (32), the second bevel gear (33) meshes with the corresponding first bevel gear (31), a winding wheel (34) is fixedly sleeved on the two fixed rods (32), a pull rope (35) is wound around the two winding wheels (34), two second springs (37) are fixedly mounted on the top of the anti-seismic bottom plate (1), anti-slipping plates (36) are fixedly mounted on the top ends of the two second springs (37), and the top ends of the pull ropes (35) are fixedly connected to the bottom ends of the corresponding anti-slipping plates (36).

4. The cell storage device with heat preservation and refrigeration function according to claim 2, characterized in that: An auxiliary groove is provided on the inner wall of the installation groove, and an auxiliary block is fixedly installed on one side of the buffer arm (29), and the auxiliary block is rotatably connected in the auxiliary groove.

5. The cell storage device with heat preservation and refrigeration function according to claim 1, characterized in that: Two limiting grooves (21) are provided on one side of the two fixing frames (4), and grooves are provided on the inner walls of one side of the two fixing grooves. Limiting blocks (22) are slidably installed in the two grooves, and the limiting blocks (22) are installed in the corresponding limiting grooves (21).

6. The cell storage device with heat preservation and refrigeration function according to claim 5, characterized in that: A second rack is fixedly mounted on one side of the two limit blocks (22); a fixing cavity is provided on the cover plate (6); two adjustment holes are provided on the bottom inner wall of the fixing cavity; adjustment rods (24) are rotatably mounted in the two adjustment holes; second gears (23) are fixedly mounted at the bottom ends of the two adjustment rods (24); and the second gears (23) are meshed with the corresponding second racks.

7. The cell storage device with heat preservation and refrigeration function according to claim 6, characterized in that: A third bevel gear is fixedly mounted on the top of the two adjusting rods (24), two rotating rods (25) are rotatably mounted in the fixed cavity, a fourth bevel gear is fixedly mounted on the ends of the two rotating rods (25) that are away from each other, and the fourth bevel gear is meshed with the corresponding third bevel gear, and a same worm gear (26) is fixedly mounted on the ends of the two rotating rods (25) that are close to each other, a round rod is rotatably mounted on the inner wall of the top of the fixed cavity, a worm (27) is fixedly mounted on the bottom end of the round rod, and the worm (27) is meshed with the worm gear (26), and a switch dial (28) is fixedly mounted on the top end of the round rod.

8. A method for using the cell storage device with heat preservation and refrigeration function as claimed in any one of claims 1 to 7, characterized in that: The following steps are involved: S1: Pull the stabilizing plate (13), the stabilizing plate (13) drives the sliding rod (11) to move, the sliding rod (11) drives the bottom plate to move and squeeze the first spring (12), the first spring (12) undergoes elastic deformation, and multiple culture bottles (19) are placed on the placement plate (10), the stabilizing plate (13) is released, the elastic action of the first spring (12) can drive the stabilizing plate (13) to reset and move, the stabilizing plate (13) clamps and fixes the multiple culture bottles (19), and at the same time the first gear (17) moves and rotates on the first rack (18), so that the first gear (17) drives the screw rod (15) to rotate, the screw rod (15) drives another screw rod (15) to rotate through the rotating wheel (16), so that the screw rod (15) drives the corresponding clamping plate (14) to move, the two clamping plates (14) can stabilize the two sides of the culture bottle (19), and can better limit the position of the culture bottle (19); S2: When it is necessary to cold store the stem cell sample at a lower temperature, the cover plate (6) is moved downward to the bottom, the cover plate (6) drives the two fixed plates (5) to move, the fixed plates (5) slide on the corresponding fixed frames (4), the fixed plates (5) drive the placement plates (10) and the plurality of culture bottles (19) to enter the storage box (3), the switch dial (28) is turned, the switch dial (28) drives the round rod to rotate, the round rod drives the worm (27) to rotate, the worm (27) controls the worm wheel (26) to rotate, the worm wheel (26) can drive the two rotating rods (25) to rotate, the rotating rods (25) control the fourth bevel gear to rotate, the fourth bevel gear drives the corresponding third bevel gear The adjusting rod (24) on the upper part rotates, the adjusting rod (24) can drive the corresponding second gear (23) to rotate, the second gear (23) drives the corresponding limit block (22) to move, the limit block (22) slides into the corresponding limit groove (21), and can fix the position of the cover plate (6), the insulation plate (20) can improve the insulation effect in the storage box (3), and in the process of the movable cover plate (6) driving the fixed plate (5) to descend, the volume of the first cavity in the fixed plate (5) is compressed, so that the liquid nitrogen preparation is squeezed into the second cavity of each placement plate (10), and overflows from each sample installation hole to contact the culture bottle (19) thereon, thereby realizing low temperature storage; If it is necessary to store other samples at a higher suitable temperature, the movable cover plate (6) is moved to the top. Since the placement plate (10) is designed to be high in the middle and low on both sides, the liquid nitrogen preparation automatically flows back into the first cavity. If it is necessary to store multiple samples, some at lower temperatures and some at higher temperatures, the cover plate (6) is moved downward to the middle position of the maximum descending stroke, and the stem cells that require a lower temperature are placed on the placement plate (10) at the bottom, and other types of samples are placed on the placement plates (10) at a higher level, so that the liquid nitrogen preparation can only enter the second cavity in the placement plates (10) at a lower level. In this process, the semiconductor refrigeration sheet (8) cools the storage box (3), and cooperates with the two fans (9) to drive the air flow in the storage box (3), so that the cooling speed is faster. S3: The storage box (3) can provide good protection for the culture bottle (19), and when the storage box (3) shakes during transportation, the storage box (3) will squeeze the rollers (30) on the two buffer arms (29). After being squeezed, the buffer arms (29) drive the first bevel gear (31) to rotate, the first bevel gear (31) drives the second bevel gear (33) to rotate, the second bevel gear (33) drives the fixed rod (32) to rotate, the fixed rod (32) drives the winding wheel (34) to rotate, the winding wheel (34) can reel in the pull rope (35), so that the pull rope (35) pulls the anti-slip sheet (36), the anti-slip sheet (36) squeezes the second spring (37), the elastic action of the second spring (37) can drive the anti-slip sheet (36) to reset, and provide a good buffering effect for the buffer arm (29), thereby playing a shock-absorbing and buffering role for the storage box (3).

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