A zebrafish temperature-sensitive behavior trajectory monitoring device and monitoring method

Abstract

The present invention discloses a zebrafish temperature-sensitive behavior trajectory monitor and monitoring method, which is provided with a monitoring box, the inner bottom surface of which is provided with a heating box, and the interior of the monitoring box is provided with a warm zone, a normal temperature zone and a low temperature zone from left to right, respectively, and a monitor is installed on the outside of the monitoring box; a box cover is clamped and arranged on the top of the monitoring box, and a fan is installed on the upper end surface of the box cover; it includes: a fixed pipe, the top of which is fixedly connected to the bottom of the fan, and the sides of the fixed pipe are connected to each other through a connecting pipe and a supporting block; a partition plate is fixed inside the monitoring box and serves to separate the warm zone, the normal temperature zone and the low temperature zone, and the two ends of the partition plate are provided with fixed cavities, and a connection channel is reserved inside the partition plate. The zebrafish temperature-sensitive behavior trajectory monitor can reduce the mixing between water flows in different areas by partitioning the inside of the monitoring box and realizing heat insulation of adjacent partitioned areas, thereby improving the accuracy of monitoring data.

Description

Technical Field

[0001] The invention relates to the technical field of zebrafish, and in particular to a zebrafish temperature-sensitive behavior trajectory monitoring device and a monitoring method. Background Art

[0002] When zebrafish are being cultivated, their trajectory needs to be monitored based on their temperature-sensitive behavior. Usually, the zebrafish are placed in a breeding box, and different temperatures are set in the breeding box. Then, the movement trajectory of the zebrafish is observed and analyzed and recorded. For example, a zebrafish temperature-sensitive behavior trajectory monitor and monitoring method with publication number CN203575350U includes a warm water chamber, a normal temperature water chamber, and a cold water chamber. The warm water chamber and the normal temperature water chamber are separated by a vertical partition, and the normal temperature water chamber and the cold water chamber are separated by a vertical partition... The movement trajectory of zebrafish can be recorded, and the cold and hot effects of trace components of traditional Chinese medicine can be monitored. However, the zebrafish temperature-sensitive behavior trajectory monitor still has the following shortcomings in actual use:

[0003] Most of the water flows inside traditional monitoring instruments are connected, so the temperature of different areas cannot be well controlled during monitoring. In addition, the back and forth movement of zebrafish will cause the water in different areas to mix, resulting in inaccurate monitoring data. To address the above problems, it is urgently necessary to carry out innovative designs based on the original trajectory monitoring instrument. Summary of the invention

[0004] The purpose of the present invention is to provide a zebrafish temperature-sensitive behavior trajectory monitor and monitoring method to solve the problem proposed in the above background technology that most of the internal water flow of traditional monitoring instruments is in a connected state, so the temperature of different areas cannot be well controlled during monitoring, and the back and forth movement of zebrafish will cause the water in different areas to mix, thereby causing inaccurate monitoring data.

[0005] To achieve the above object, the present invention provides the following technical solution: a zebrafish temperature-sensitive behavior trajectory monitor, provided with a monitoring box, the inner bottom surface of the monitoring box is equipped with a heating box, and the interior of the monitoring box is respectively provided with a warm temperature zone, a normal temperature zone and a low temperature zone from left to right, and a monitor is installed on the outer side of the monitoring box;

[0006] A box cover is snap-fitted on the top of the monitoring box, and a fan is installed on the upper end surface of the box cover;

[0007] include:

[0008] A fixed pipe, the top of which is fixedly connected to the bottom of the fan, and the sides of the fixed pipe are connected to each other through a connecting pipe and a support block;

[0009] The partition plate is fixed inside the monitoring box and serves to separate the warm temperature zone, the normal temperature zone and the low temperature zone. Fixed cavities are opened at both ends of the partition plate, and a connecting channel is reserved inside the partition plate, and sleeve blocks are fixed on both sides of the partition plate.

[0010] Preferably, the bottom of the fixed tube extends to the lower end of the normal temperature zone, and the top of the fixed tube is connected to each other through a connecting tube and a support block, so that the air blown out of the fixed tube can directly extend into the bottom of the normal temperature zone, thereby driving the water flow.

[0011] Preferably, a fixing groove is provided on the side of the support block, and the support block and the connecting tube are snap-connected, and the outer diameter of the connecting tube is equal to the inner diameter of the support block. When the box cover is closed, the connecting tube can be driven to be stuck into the interior of the support block, thereby realizing the connection between the connecting tube and the transmission tube.

[0012] Preferably, the end of the support block is fixedly connected to one end of the transmission pipe, and the other end of the transmission pipe is connected to each other through the mounting block and the exhaust pipe, and the exhaust pipe is arranged in both the warm zone and the low-temperature zone. The arrangement of the exhaust pipe can drive the fluctuation of water flow in the warm zone and the low-temperature zone respectively.

[0013] Preferably, the transmission pipe and the exhaust pipe are both threadedly connected to the mounting block, and the threads on the transmission pipe and the exhaust pipe are in opposite directions, and the exhaust pipe is an inverted "L"-shaped structure. By rotating the mounting block, the transmission pipe and the exhaust pipe can be connected to achieve gas connectivity.

[0014] Preferably, the interior of the fixed cavity is filled with insulating material, the inner diameter of the sleeve block is larger than the inner diameter of the connecting channel, and the sleeve block is trumpet-shaped. The setting of the partition plate can well isolate the temperature between different areas, and at the same time will not affect the swimming of zebrafish through the connecting channel.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: the zebrafish temperature-sensitive behavior trajectory monitor can reduce the mixing of water flows in different areas by partitioning the monitoring box and achieving heat insulation of adjacent partitioned areas, thereby improving the accuracy of monitoring data;

[0016] 1. The partition plate can be used to isolate different temperature areas, and the filling of thermal insulation materials inside the fixed cavity can reduce the temperature conduction between different areas. At the same time, the setting of the connecting channel will not affect the swimming of zebrafish. The setting of the set block on the partition plate facilitates the entry of zebrafish and reduces the mixed flow of water.

[0017] 2. The installation between the transmission pipe and the exhaust pipe can be achieved by rotating the mounting block, so that the wind blown out by the fan can enter the normal temperature zone through the fixed pipe, or enter the warm zone and the low temperature zone respectively through the connecting pipe, the transmission pipe and the exhaust pipe, which can not only realize the fluctuation of water to ensure the comfort of zebrafish, but also ensure the uniformity of water temperature in different areas. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the front cross-section structure of the present invention;

[0019] Figure 2 This is a schematic diagram of the front cross-sectional structure of the sleeve block of the present invention;

[0020] Figure 3 This is a schematic diagram of the front cross-sectional structure of the partition plate of the present invention;

[0021] Figure 4 This is a schematic diagram of the three-dimensional structure of the support block of the present invention;

[0022] Figure 5 This is a schematic diagram of the front cross-section structure of the mounting block of the present invention.

[0023] In the figure: 1. Monitoring box; 2. Heating box; 3. Warm zone; 4. Normal temperature zone; 5. Low temperature zone; 6. Monitor; 7. Box cover; 8. Fan; 9. Fixed pipe; 10. Connecting pipe; 11. Support block; 111. Fixed groove; 12. Transmission pipe; 13. Mounting block; 14. Exhaust pipe; 15. Partition plate; 16. Fixed cavity; 17. Connecting channel; 18. Set block. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] See also Figure 1-5 The present invention provides a technical solution: a zebrafish temperature-sensitive behavior trajectory monitor, which is provided with a monitoring box 1, a heating box 2 is installed on the inner bottom surface of the monitoring box 1, and a warm zone 3, a normal temperature zone 4 and a low temperature zone 5 are respectively arranged inside the monitoring box 1 from left to right, and a monitor 6 is installed on the outer side of the monitoring box 1;

[0026] A box cover 7 is snap-fitted on the top of the monitoring box 1, and a fan 8 is installed on the upper end surface of the box cover 7;

[0027] include:

[0028] A fixed pipe 9, the top of which is fixedly connected to the bottom of the fan 8, and the sides of the fixed pipe 9 are connected to each other through a connecting pipe 10 and a support block 11;

[0029] The partition plate 15 is fixed inside the monitoring box 1 and serves to separate the warm zone 3, the normal temperature zone 4 and the low temperature zone 5. Fixed cavities 16 are provided at both ends of the partition plate 15, and a connecting channel 17 is reserved inside the partition plate 15, and sleeve blocks 18 are fixed on both sides of the partition plate 15.

[0030] The bottom of the fixed tube 9 extends to the lower end of the normal temperature zone 4, and the top of the fixed tube 9 is interconnected with the support block 11 through the connecting tube 10; a fixing groove 111 is provided on the side of the support block 11, and the support block 11 and the connecting tube 10 are snap-fitted, and the outer diameter of the connecting tube 10 is equal to the inner diameter of the support block 11; the end of the support block 11 is fixedly connected to one end of the transmission tube 12, and the other end of the transmission tube 12 is connected to the exhaust pipe 14 through the mounting block 13, and the exhaust pipe 14 is provided in both the warm zone 3 and the low temperature zone 5; the transmission tube 12 and the exhaust pipe 14 are both threadedly connected to the mounting block 13, and the threads on the transmission tube 12 and the exhaust pipe 14 are in opposite directions, and the exhaust pipe 14 is an inverted "L" shaped structure; Figure 1 and Figure 4-5 As shown, the transmission pipe 12 and the exhaust pipe 14 can be connected by rotating the mounting block 13, and the wind can enter the normal temperature zone 4 through the fixed pipe 9, and the other wind can enter the transmission pipe 12 through the connecting pipe 10, and finally be discharged from the exhaust pipe 14 to the warm temperature zone 3 and the low temperature zone 5 respectively, and drive the water flow to move;

[0031] The interior of the fixed cavity 16 is filled with heat insulating material, the inner diameter of the sleeve block 18 is larger than the inner diameter of the connecting channel 17, and the sleeve block 18 is trumpet-shaped; Figure 1-3 As shown, filling the fixed cavity 16 with heat insulating material can improve the heat insulating performance of the partition plate 15, reduce the heat conduction between different temperature areas, and thus improve the accuracy of the monitoring data.

[0032] A zebrafish temperature-sensitive behavior trajectory monitoring device and monitoring method: Figure 1-3 As shown, before monitoring, the transmission pipe 12 and the exhaust pipe 14 are connected by rotating the mounting block 13, so as to achieve communication between the two. In this way, after the box cover 7 is closed, the connecting pipe 10 can be driven to engage with the inside of the supporting block 11 to achieve communication between the two. The wind force generated by the fan 8 can enter the normal temperature zone 4 through the fixed pipe 9 and drive the water flow to move. The other wind force can enter the transmission pipe 12 through the connecting pipe 10, and finally be discharged from the exhaust pipe 14 to the warm zone 3 and the low temperature zone 5 respectively, and drive the water flow to move, which can not only improve the comfort of zebrafish, but also ensure the uniformity of water temperature in different areas.

[0033] Three areas with different temperatures are set in the monitoring box 1 to monitor the temperature-sensitive behavior of zebrafish. The warm zone 3, the normal temperature zone 4 and the low temperature zone 5 are separated by a partition plate 15, and the fixed cavity 16 is filled with insulation material, which can improve the insulation performance of the partition plate 15, reduce the heat conduction between different temperature zones, and thus improve the accuracy of the monitoring data. A connecting channel 17 is set on the partition plate 15, and the zebrafish swims between the warm zone 3, the normal temperature zone 4 and the low temperature zone 5 through the connecting channel 17. The behavior trajectory of the zebrafish in the three different temperature zones is monitored in real time by the monitor 6. Since the inner diameter of the connecting channel 17 is smaller than the inner diameter of the sleeve block 18, it is convenient for the zebrafish to enter and can reduce the mixing of water flow in different areas.

[0034] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A zebrafish temperature-sensitive behavior trajectory monitor, comprising a monitoring box (1), a heating box (2) being installed on the inner bottom surface of the monitoring box (1), and a warm temperature zone (3), a normal temperature zone (4) and a low temperature zone (5) being respectively arranged inside the monitoring box (1) from left to right, and a monitor (6) being installed on the outer side of the monitoring box (1); A box cover (7) is snap-fitted to the top of the monitoring box (1), and a fan (8) is installed on the upper end surface of the box cover (7); It is characterized in that include: A fixed pipe (9), the top of which is fixedly connected to the bottom of the fan (8), and the sides of the fixed pipe (9) are connected to each other via a connecting pipe (10) and a support block (11); after the box cover (7) is closed, the box cover (7) can drive the connecting pipe (10) to snap into the inside of the support block (11), thereby achieving communication between the two; A partition plate (15) is fixed inside the monitoring box (1) and serves to separate the warm temperature zone (3), the normal temperature zone (4) and the low temperature zone (5); fixing cavities (16) are provided at both ends of the partition plate (15); a connecting channel (17) is reserved inside the partition plate (15); and sleeve blocks (18) are fixed on both sides of the partition plate (15); the inner diameter of the sleeve block (18) is larger than the inner diameter of the connecting channel (17), and the sleeve block (18) is trumpet-shaped; The bottom of the fixed tube (9) extends to the lower end of the normal temperature zone (4), and the top of the fixed tube (9) is connected to the support block (11) via the connecting tube (10); A fixing groove (111) is provided on the side of the support block (11), the support block (11) and the connecting pipe (10) are snap-fitted and connected, and the outer diameter of the connecting pipe (10) is equal to the inner diameter of the support block (11); The end of the support block (11) is fixedly connected to one end of the transmission pipe (12), and the other end of the transmission pipe (12) is connected to the exhaust pipe (14) via the mounting block (13); The exhaust pipe (14) is arranged in both the warm zone (3) and the low temperature zone (5); The transmission pipe (12) and the exhaust pipe (14) are both threadedly connected to the mounting block (13), the threads on the transmission pipe (12) and the exhaust pipe (14) are in opposite directions, and the exhaust pipe (14) is an inverted "L"-shaped structure.

2. A zebrafish temperature-sensitive behavior trajectory monitor according to claim 1, characterized in that: The interior of the fixing cavity (16) is filled with heat insulation material.

3. A monitoring method based on the zebrafish temperature-sensitive behavior trajectory monitor according to claim 1: before monitoring, the transmission pipe (12) and the exhaust pipe (14) are connected by rotating the mounting block (13) to achieve communication between the two, and the wind force generated by the fan (8) can enter the normal temperature zone (4) through the fixed pipe (9) and drive the water flow to move, and the other wind force can enter the transmission pipe (12) through the connecting pipe (10), and finally be discharged from the exhaust pipe (14) to the warm zone (3) and the low temperature zone (5) respectively, driving the water flow to move, which can improve the comfort of the zebrafish and ensure the uniformity of the water temperature in different areas; Three areas with different temperatures are arranged in the monitoring box (1) so as to monitor the temperature-sensitive behavior of zebrafish. The warm area (3), the normal temperature area (4) and the low temperature area (5) are separated by a partition plate (15). A heat-insulating material is filled in the fixed cavity (16) to improve the heat-insulating performance of the partition plate (15), reduce the conduction of heat between the different temperature areas, and thus improve the accuracy of the monitoring data. A connecting channel (17) is arranged on the partition plate (15). The zebrafish swims between the warm area (3), the normal temperature area (4) and the low temperature area (5) through the connecting channel (17). The behavior trajectory of the zebrafish in the three different temperature areas is monitored in real time by the monitoring instrument (6). Since the inner diameter of the connecting channel (17) is smaller than the inner diameter of the sleeve (18), the zebrafish can enter conveniently and the mixing of water flow in different areas can be reduced.

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