A cell compatibility testing system and method for preparing bionic bone based on composite materials

Abstract

The present invention provides a cell compatibility test system for preparing bionic bone based on composite materials, comprising: a laboratory table, on which a plurality of experimental units are arranged, each of which is used to perform a cell compatibility test on the bionic bone; an information acquisition module, the information detection module is used to perform information acquisition on the experimental units on the laboratory table; a controller, the controller is used to record the information collected by the information acquisition module for multiple times, and to compare the information collected multiple times with the pre-stored information and obtain the cell compatibility test results. The present invention is used to uniformly acquire, record and test information when bionic bone is implanted in experimental animals, further improve the experimental efficiency, and at the same time, the validity of the experimental results is effectively improved by uniformly collecting data or controlling the experimental animals through the system.

Description

Technical Field

[0001] The invention relates to the technical field of bionic bone, and in particular to a cell compatibility testing system and method for preparing bionic bone based on composite materials. Background Art

[0002] Bone defect is one of the most common diseases in orthopedic clinics. According to statistics, 7 people in my country suffer serious disability due to traffic accidents every minute, and there are more than 10 million patients with bone defects every year. Bone defect repair and reconstruction has always been an international clinical problem. Traditional metals and polymer materials have postoperative complications such as uncontrollable bionic structures, mismatched mechanical properties, poor biocompatibility, no developmental function, movement dislocation, and wear. In particular, prostheses without biological activity cannot develop in the human body and cannot fuse well with natural bones, requiring secondary surgery to repair;

[0003] With the emergence of 3D printing technology, 3D printed bones made of bioceramics have become recognized as the most ideal bone filling material. In recent years, foreign research institutions have developed 3D printed bioceramic bone implant medical devices. Due to the use of acidic binders and functional gradients, this technology has not yet achieved complete degradation of ceramic bones, and will cause severe pain and other side effects to patients after implantation;

[0004] Therefore, bionic bone is studied as a new technology and material by various research groups. For example, Tang Ruikang's research group of Zhejiang University's Department of Chemistry used bionics to prepare a new organic-inorganic composite elastic crystal material, whose physical and chemical properties are close to those of natural bone. However, although the bionic bone has been successfully tested in terms of cell compatibility, it has not been promoted to clinical use.

[0005] The main reason is that although bionic bone implantation can be successful in experimental animals, human implantation is still in the exploratory stage. During the verification process of bionic bone implantation in experimental animals, there is a lack of a system that can conduct unified testing to record and analyze data in a unified manner. Summary of the invention

[0006] The present invention provides a cell compatibility testing system and method for preparing bionic bone based on composite materials, which are used for uniformly acquiring, recording and testing information when bionic bone is implanted in experimental animals, thereby further improving the experimental efficiency. At the same time, the validity of the experimental results is effectively improved by uniformly collecting data or controlling the experimental animals through the system.

[0007] A cell compatibility test system for preparing bionic bone based on composite materials, comprising: a test bench, on which a plurality of experimental units are arranged, each of which is used to perform a cell compatibility test on the bionic bone;

[0008] An information collection module, wherein the information detection module is used to collect information from the test units on the test bench;

[0009] A controller is used to record the information collected by the information collection module for multiple times, and to compare the information collected for multiple times with the pre-stored information to obtain the cell compatibility experiment result.

[0010] Preferably, the method further comprises a terminal, wherein the terminal is used to output or display the cell compatibility experiment results obtained by the controller.

[0011] Preferably, the information acquisition module is used to acquire osteoblast compatibility information of the bionic bone and transmit the compatibility information to the controller;

[0012] The osteoblast compatibility information includes: cell proliferation effect detection information and alkaline phosphatase activity information.

[0013] Preferably, the information collection module is used to collect cell proliferation effect detection information, and collects experimental information of multiple time periods for the cell proliferation effect detection information of each experimental unit;

[0014] Furthermore, the information collection module is further used to collect alkaline phosphatase activity information, and collect experimental information of multiple time periods for the alkaline phosphatase activity information of each experimental unit.

[0015] Preferably, the experimental table comprises: an experimental unit, the experimental unit is mounted on the upper surface of the third bottom plate through a plurality of first clamping devices, the upper surface of the third bottom plate is symmetrically provided with a plurality of first clamping devices, the first clamping device comprises a second bracket, the second bracket is a T-shaped structure, the lateral end of the T-shaped structure of the second bracket is fixed to the upper surface of the third bottom plate, the vertical end of the second bracket is provided with a motor, the motor faces a side of the experimental unit, the output end of the motor is connected to a push rod, the end of the push rod away from the output end of the motor is connected to a clamp block of an L structure, the L opening of the clamp block is arranged downward; the side of the clamp block close to the experimental unit is used to abut against the experimental unit;

[0016] A first partition and a second partition are provided in the experimental unit, the first partition and the second partition respectively isolate the experimental unit to form a first experimental unit, a second experimental unit and a third experimental unit, and a first pipe, a second pipe, a third pipe and a fourth pipe are arranged at intervals on one side of the first experimental unit away from the second experimental unit and the third experimental unit;

[0017] A third bracket is arranged at intervals on the side of the second test unit and the third test unit away from the first test unit, the third bracket is used to set up the first connecting rod, and a camera is arranged on the lower surface of one end of the first connecting rod away from the third bracket; the camera is used to collect video or image information in the experimental unit.

[0018] Preferably, the fourth pipe penetrates through the side wall of the first test unit and extends into the second test unit, a fifth pipe is further provided on a side of the second test unit away from the first test unit, and fixing components are provided on both sides of the third bottom plate, and the fixing components are used to fix the third bottom plate on the laboratory bench.

[0019] The fixing assembly comprises: a first bracket and a fourth bracket, wherein the first bracket is spaced apart and mounted on the second bottom plate through a fourth base, and the fourth bracket is spaced apart and mounted on the second bottom plate through a third base, and a fourth bottom plate is further disposed between the first bracket and the fourth bracket, and the upper surface of the fourth bottom plate is used to connect to the third bottom plate;

[0020] Fixed plates are arranged at intervals below the fourth bottom plate, the fixed plates are used to connect the first chuck and the second chuck, one side of the first chuck is used to connect with the first bracket, the other side of the first chuck is used to clamp the third bottom plate, one side of the second chuck is connected with the fourth bracket, the other side of the second chuck and the first chuck cooperate to clamp the third bottom plate;

[0021] A shaft sleeve is provided on a side of the first bracket away from the first chuck, a first rotating shaft is provided in the shaft sleeve, the first rotating shaft passes through the first bracket, and the first rotating shaft is rotatably connected to the first chuck, and the first rotating shaft is used to adjust the gap between the first chuck and the second chuck.

[0022] Preferably, a plurality of clamping blocks are provided on the third base, and each of the clamping blocks is used to clamp the fourth bracket on the upper top surface of the third base;

[0023] A control box is also provided on one side of the third base, wherein a controller is provided in the control box, and the controller is electrically connected to the camera;

[0024] The third bottom plate is also provided with a first clamping device, and a plurality of the first clamping devices are provided and are spaced apart from each other on the third bottom plate.

[0025] A second base is further provided between two adjacent first clamping devices, and a first insertion rod is provided on the upper top surface of the second base, and the first insertion rod is used to cooperate with the positioning cylinder on the lower bottom surface of the test unit;

[0026] A plurality of lifting devices are also arranged at intervals on one side of the first insertion rod close to the center of the test unit, the fixed ends of the lifting devices are connected to the third bottom plate, and the movable ends of the lifting devices face upward and abut against the lower bottom surface of the test unit;

[0027] A first notch is provided on the lower bottom surface of the experimental unit, fourth partitions are symmetrically provided at both ends of the first notch, a plurality of third partitions are spaced between the fourth partitions, and the fourth partitions and the third partitions are respectively used for clamping at the clamping ends of the first clamping device.

[0028] Preferably, the first clamping device comprises: a first base, the lower part of the first base is mounted on the upper surface of the third bottom plate, a vertical plate and a trigger platform are arranged at intervals on the upper surface of the first base, a second hinge shaft is arranged at intervals on one side of the vertical plate, clamping rods are respectively hinged on the second hinge shafts, a spring is arranged at one end of the clamping rods close to the second hinge shaft, a side of one of the clamping rods away from the vertical plate is rotatably connected to the second connecting rod through the first hinge shaft, and the other end of the second connecting rod abuts against or releases the trigger end of the trigger platform according to the clamping state of the two clamping rods;

[0029] The second connecting rod is also provided with a shift lever, the shift lever extending from the second connecting rod to the outer side wall of one of the clamping rods, and the shift lever is located between the trigger platform and the first hinge shaft;

[0030] The upper surface of the third bottom plate is also provided with a first discharge pipe and a second discharge pipe at intervals, the first discharge pipe and the second discharge pipe are respectively connected to the output ends of the drainage pipe, and the end of the drainage pipe away from the sixth pipe and the seventh pipe passes through the fourth bracket and the second chuck, and extends to the inside of the third bottom plate.

[0031] Preferably, the bottom surfaces of the third base and the fourth base are respectively mounted on the upper surface of the second base plate through transverse slide rails, and the lower side of the second base plate is movably mounted on the upper surface of the first base plate through longitudinal slide rails;

[0032] The second chuck and the fourth bracket are both provided with a through pipeline channel, the pipeline channel is used for the drainage pipe to pass through, one end of the drainage pipe is connected to the sixth pipeline and the seventh pipeline; the other end of the drainage pipe is respectively connected to the first discharge pipe and the second discharge pipe;

[0033] The sixth pipe and the seventh pipe are respectively arranged in the second notch of the support rod, one end of the support rod away from the second notch is mounted in the L notch of the second angle iron, and one side of the second angle iron is fixed to the side wall of the third base;

[0034] The opening edge of the experimental unit is provided with a sealing edge, and the sealing edge is used to cooperate with the sealing cover and to seal the opening of the experimental unit;

[0035] The outer wall of the shaft sleeve is provided with a slide groove, and the slide groove is used to install one side of the first angle iron, and the other side of the first angle iron is connected to the outer wall of the first bracket.

[0036] Preferably, the method further comprises the following:

[0037] The trigger station is used to trigger the valve provided on the sixth pipeline or the seventh pipeline;

[0038] When the first clamping device clamps the third bottom plate, the second connecting rod on the first clamping device will trigger the triggering platform, and the triggering platform will start the valves connected to the sixth pipeline and the seventh pipeline;

[0039] During the experiment, the valves of the sixth pipeline and the seventh pipeline are started according to the test environment, including: when the test temperature is lower than the first preset value, the valve of the sixth pipeline is started; when the test temperature is higher than the second preset value, the valve of the seventh pipeline is started.

[0040] The beneficial effects of the present invention are as follows:

[0041] The present invention provides a cell compatibility test system for preparing bionic bone based on composite materials, comprising: a laboratory table, on which a plurality of experimental units are arranged, each of which is used to perform a cell compatibility test on the bionic bone; an information acquisition module, the information detection module is used to perform information acquisition on the experimental units on the laboratory table; a controller, the controller is used to record the information collected by the information acquisition module for multiple times, and to compare the information collected multiple times with the pre-stored information and obtain the cell compatibility test results. The present invention is used to uniformly acquire, record and test information when bionic bone is implanted in experimental animals, further improve the experimental efficiency, and at the same time, the validity of the experimental results is effectively improved by uniformly collecting data or controlling the experimental animals through the system.

[0042] Other features and advantages of the present invention will be described in the following description, and partly become apparent from the description, or understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures particularly pointed out in the written description and the accompanying drawings.

[0043] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0045] Figure 1 It is a structural schematic diagram of the present invention;

[0046] Figure 2 It is a schematic diagram of the structure of the experimental platform of the present invention;

[0047] Figure 3 It is a schematic diagram of the experimental unit connection structure of the present invention;

[0048] Figure 4 Another schematic diagram of the three-dimensional structure of the experimental unit of the present invention;

[0049] Figure 5 It is a schematic diagram of the first bottom plate connection structure of the present invention;

[0050] Figure 6 Another structural schematic diagram of the first bottom plate of the present invention;

[0051] Figure 7 It is a schematic diagram of the support rod structure of the present invention;

[0052] Figure 8 It is a schematic structural diagram of the first clamping device of the present invention;

[0053] Among them, 1-first bottom plate, 2-second bottom plate, 3-third bottom plate, 4-first pipeline, 5-second pipeline, 6-third pipeline, 7-fourth pipeline, 8-sleeve, 9-first bracket, 10-first chuck, 11-second bracket, 12-third bracket, 13-first connecting rod, 14-experimental unit, 15-first partition, 16-second chuck, 17-fourth bracket, 18-first clamping device, 19-sealing edge, 20-first test unit, 21-second partition, 22-second test unit, 23-third test unit, 24-clamping block, 25-top rod, 26-fifth pipeline, 27-temperature and humidity sensor, 28-first notch, 29-third partition, 30-fourth partition, 31-second connecting rod, 32-clamping rod, 33-first hinge axis, 34-second hinge axis, 35-vertical plate, 36-gear lever, 37-trigger platform,

[0054] 38-first base, 39-first plug rod, 40-second base, 41-lifting device, 42-positioning cylinder, 43-first angle iron, 44-first rotating shaft, 46-control box, 47-sixth pipeline, 48-seventh pipeline, 49-drainage pipe, 50-pipeline channel, 51-support rod, 52-second angle iron, 53-third base, 54-block, 55-fourth bottom plate, 56-fixed plate, 57-fourth base, 58-second notch, 59-first discharge pipe, 60-second discharge pipe, 61-spring. DETAILED DESCRIPTION

[0055] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0056] according to Figure 1-8 As shown, an embodiment of the present invention provides a cell compatibility test system for preparing bionic bone based on composite materials, comprising: a test bench, on which a plurality of experimental units 14 are arranged, each of the experimental units 14 is used to perform a cell compatibility test on the bionic bone;

[0057] An information collection module, wherein the information detection module is used to collect information from the test units on the test bench;

[0058] A controller is used to record the information collected by the information collection module for multiple times, and to compare the information collected for multiple times with the pre-stored information to obtain the cell compatibility experiment result.

[0059] The present invention provides a cell compatibility testing system and method for preparing bionic bone based on composite materials, which are used for uniformly acquiring, recording and testing information when bionic bone is implanted in experimental animals, thereby further improving the experimental efficiency. At the same time, the validity of the experimental results is effectively improved by uniformly collecting data or controlling the experimental animals through the system.

[0060] In the present invention, the data of the information acquisition module can be collected multiple times to achieve the purpose of monitoring the cell compatibility of the bionic bone in the entire test stage. At the same time, the experimental information of multiple monitoring time points can be extracted according to the monitoring information, so that the experimental results can be personalized according to the experimental state of the bionic bone. The purpose of the calculation result is to effectively improve the experimental efficiency and experimental effectiveness.

[0061] In one embodiment, it further includes a terminal, wherein the terminal is used to output or display the cell compatibility experiment results obtained by the controller.

[0062] The information acquisition module is used to acquire osteoblast compatibility information of the bionic bone and transmit the compatibility information to the controller. The osteoblast compatibility information includes: cell proliferation effect detection information and alkaline phosphatase activity information.

[0063] The information collection module is used to collect cell proliferation effect detection information, and collects experimental information of multiple time periods for the cell proliferation effect detection information of each experimental unit.

[0064] The information collection module is also used to collect alkaline phosphatase activity information, and collects experimental information of alkaline phosphatase activity information of each experimental unit 14 in multiple time periods.

[0065] In this embodiment, the terminal is any one of a computer, a mobile phone or a tablet computer; when working, the information acquisition module can be a temperature and humidity sensor 27 and a camera, the temperature and humidity sensor 27 is used to monitor the temperature and humidity in the experimental unit 14 of the experimental table, and the camera is used to regularly collect photos or video information in the experimental unit 14, and further obtain various information on osteoblast compatibility based on the photos or video information; it is convenient for experimental personnel to uniformly check the osteoblast compatibility information. It reduces the situation of manual recording and manual flipping through the record sheet to check the experimental information, and greatly improves the experimental efficiency. Further, the experimental information is obtained by computer calculation, and multiple bar graphs or line graphs are generated during the experiment based on the experimental information. The experimental process is analyzed using the bar graph or line graph to obtain the experimental results of osteoblast compatibility.

[0066] In one embodiment, according to Figure 1-8 As shown, the experimental table includes: an experimental unit 14, the experimental unit 14 is mounted on the upper surface of the third bottom plate 3 through a plurality of first clamping devices 18, the upper surface of the third bottom plate 3 is symmetrically provided with a plurality of first clamping devices 18, the first clamping device 18 includes a second bracket 11, the second bracket 11 is a T-shaped structure, the lateral end of the T-shaped structure of the second bracket 11 is fixed to the upper surface of the third bottom plate 3, the vertical end of the second bracket 11 is provided with a motor, the motor faces a side of the experimental unit 14, the output end of the motor is connected to a push rod 25, the end of the push rod 25 away from the output end of the motor is connected to a clamp block 24 of an L structure, and the L opening of the clamp block 24 is arranged downward; the side of the clamp block 24 close to the experimental unit 14 is used to abut against the experimental unit 14;

[0067] The experimental unit 14 is provided with a first partition 15 and a second partition 21, the first partition 15 and the second partition 21 respectively isolate the experimental unit 14 to form a first experimental unit 20, a second experimental unit 22 and a third experimental unit 23, and a first pipeline 4, a second pipeline 5, a third pipeline 6 and a fourth pipeline 7 are arranged at intervals on one side of the first experimental unit 20 away from the second experimental unit 22 and the third experimental unit 23;

[0068] A third bracket 12 is arranged at intervals on one side of the second test unit 22 and the third test unit 23 away from the first test unit 20. The third bracket 12 is used to set up a first connecting rod 13. A camera is arranged on the lower surface of one end of the first connecting rod 13 away from the third bracket 12; the camera is used to collect video or image information in the experimental unit 14.

[0069] In this embodiment, the first partition 15 and the second partition 21 are used to divide the test unit into a first test unit 20, a second test unit 22 and a third test unit 23; further, each test unit can perform different experiments separately, for example, the first test unit can be used as a first experimental environment, the second test unit can be used as a second experimental environment, and the third test unit can be used as a comparative experimental group or a third experimental environment; the purpose of conducting experiments simultaneously is achieved by using three groups of divided sub-test units, thereby improving the experimental efficiency.

[0070] In one embodiment, the fourth pipe 7 passes through the side wall of the first test unit 20 and extends into the second test unit 22. A fifth pipe 26 is further provided on the side of the second test unit 22 away from the first test unit 20. Fixing components are provided on both sides of the third bottom plate 3, and the fixing components are used to fix the third bottom plate 3 on the laboratory bench.

[0071] The fixing assembly includes: a first bracket 9 and a fourth bracket 17, wherein the first bracket 9 is spaced apart from each other on the second bottom plate 2 through a fourth base 57, and the fourth bracket 17 is spaced apart from each other on the second bottom plate 2 through a third base 53, and a fourth bottom plate 55 is further disposed between the first bracket 9 and the fourth bracket 17, and the upper surface of the fourth bottom plate 55 is used to connect to the third bottom plate 3;

[0072] Fixed plates 56 are arranged at intervals below the fourth bottom plate 55, and the fixed plates 56 are used to connect the first chuck 10 and the second chuck 16. One side of the first chuck 10 is used to connect with the first bracket 9, and the other side of the first chuck 10 is used to clamp the third bottom plate 3. One side of the second chuck 16 is connected to the fourth bracket 17, and the other side of the second chuck 16 and the first chuck 10 cooperate with each other to clamp the third bottom plate 3.

[0073] A shaft sleeve 8 is provided on a side of the first bracket 9 away from the first chuck 10, a first rotating shaft 44 is provided in the shaft sleeve 8, the first rotating shaft 44 passes through the first bracket 9, and the first rotating shaft 44 is rotatably connected to the first chuck 10, and the first rotating shaft 44 is used to adjust the gap between the first chuck 10 and the second chuck 16;

[0074] The third base 53 is provided with a plurality of clamping blocks 54, each of which is used to clamp the fourth bracket 17 on the upper surface of the third base 53;

[0075] A control box 46 is also disposed on one side of the third base 53. A controller is disposed in the control box 46. The controller is electrically connected to the camera.

[0076] The third bottom plate 3 is also provided with a first clamping device 18. A plurality of first clamping devices 18 are provided and are spaced apart on the third bottom plate 3.

[0077] A second base 40 is further provided between two adjacent first clamping devices 18, and a first insertion rod 39 is provided on the upper top surface of the second base 40, and the first insertion rod 39 is used to cooperate with a positioning cylinder 42 on the lower bottom surface of the test unit;

[0078] A plurality of lifting devices 41 are also arranged at intervals on one side of the first insertion rod 39 close to the center of the test unit, the fixed end of the lifting device 41 is connected to the third bottom plate 3, and the movable end of the lifting device 41 faces upward and abuts against the lower bottom surface of the test unit;

[0079] The bottom surfaces of the third base 53 and the fourth base 57 are respectively mounted on the upper surface of the second bottom plate 2 through transverse slide rails, and the lower side of the second bottom plate 2 is movably mounted on the upper surface of the first bottom plate 1 through longitudinal slide rails;

[0080] The second chuck 16 and the fourth bracket 17 are both provided with a through-going pipe channel 50, the pipe channel 50 is used for the drainage pipe 49 to pass through, one end of the drainage pipe 49 is connected to the sixth pipe 47 and the seventh pipe 48; the other end of the drainage pipe 49 is respectively connected to the first discharge pipe 59 and the second discharge pipe 60;

[0081] The sixth pipe 47 and the seventh pipe 48 are respectively arranged in the second notch 58 of the support rod 51, and one end of the support rod 51 away from the second notch 58 is mounted in the L-notch of the second angle iron 52, and one side of the second angle iron is fixed to the side wall of the third base 53;

[0082] The opening edge of the experimental unit 14 is provided with a sealing edge, and the sealing edge is used to cooperate with the sealing cover and to seal the opening of the experimental unit 14 .

[0083] The outer wall of the shaft sleeve 8 is provided with a slide groove, and the slide groove is used to install one side of the first angle iron 43, and the other side of the first angle iron 43 is connected to the outer wall of the first bracket 9.

[0084] In this embodiment, the fixing assembly is used to clamp and erect the third base plate 3, so that the experimental unit 4 can be erected at the test position in the laboratory for experiments; when the experimental table needs to be moved during the experiment, the horizontal slide rail and the longitudinal slide rail can be used to move the experimental table in the plane of the X-axis and the Y-axis. The horizontal slide rail and the longitudinal slide rail are silent slide rails, and they can achieve the purpose of fixed-distance translation when sliding, which effectively improves the platform stability of the experimental table when it needs to be shifted during the experimental operation.

[0085] In one embodiment, the first clamping device 18 comprises: a first base 38, the lower part of the first base 38 is mounted on the upper surface of the third bottom plate 3, a vertical plate 35 and a triggering platform 37 are arranged on the upper surface of the first base 38, a second hinge shaft 34 is arranged on one side of the vertical plate 35, a clamping rod 32 is hinged on each of the second hinge shafts 34, a spring 61 is arranged on one end of the clamping rod 32 close to the second hinge shaft 34, a side of one of the clamping rods 32 away from the vertical plate 35 is rotatably connected to the second connecting rod 31 through the first hinge shaft 33, and the other end of the second connecting rod 31 abuts against or releases the triggering end of the triggering platform 37 according to the clamping state of the two clamping rods 32;

[0086] The second connecting rod 31 is further provided with a shift lever 36 . The shift lever 36 extends from the second connecting rod 31 to the outer side wall of one of the clamping rods 32 , and the shift lever 36 is located between the triggering platform 37 and the first hinge shaft 33 .

[0087] The triggering station 37 is used to trigger the valve provided on the sixth pipeline 47 or the seventh pipeline 48;

[0088] The upper surface of the third base plate 3 is also provided with a first discharge pipe 59 and a second discharge pipe 60 at intervals. The first discharge pipe 59 and the second discharge pipe 60 are respectively connected to the output ends of the drainage pipe 49. The end of the drainage pipe 49 away from the sixth pipe 47 and the seventh pipe 48 passes through the fourth bracket 17 and the second chuck 16, and extends to the inside of the third base plate 3.

[0089] The lower bottom surface of the experimental unit 14 is provided with a first notch 28, and fourth partitions 30 are symmetrically arranged at both ends of the first notch 28. A plurality of third partitions 29 are arranged between the fourth partitions 30, and the fourth partitions 30 and the third partitions 29 are respectively used for clamping the clamping ends of the first clamping device 18.

[0090] The experimental unit 14 is clamped on the third base plate 3 by the first clamping device 18, so that the experimental unit 14 can be stably set up. When in use, when the first clamping device 18 clamps the third base plate 3, the second connecting rod 31 on the first clamping device 18 will trigger the triggering platform 37, that is, when the first clamping device 18 clamps the third partition plate 29 or the fourth partition plate 30, the triggering platform 37 will start the valves connected to the sixth pipeline 47 and the seventh pipeline 48 according to the preset.

[0091] During the actual experiment, the valves of the sixth pipeline 47 and the seventh pipeline 48 are started according to the test environment, including: when the test temperature is lower than the first preset value, the valve of the sixth pipeline 47 is started; when the test temperature is higher than the second preset value, the valve of the seventh pipeline 48 is started.

[0092] In this embodiment, the first clamping device can clamp the third partition and the fourth partition below the experimental unit, so that the experimental unit can be firmly fixed on the upper surface of the third bottom plate 3; further, once the first clamping device is clamped, it can trigger the platform; thereby achieving the purpose of starting the valve connected to the sixth pipeline 47 or the seventh pipeline 48.

[0093] The working principle of the present invention is as follows:

[0094] When in use, first, the first base plate 1 and the second base plate 2 are set up in the test area of ​​the laboratory, and the test area is provided with a motor for driving the second base plate 2 to reciprocate on the longitudinal track and for driving the third base 53 and the fourth base 57 to reciprocate on the transverse track;

[0095] According to the experimental requirements, the second bottom plate 2 is set at a position on the first bottom plate 1, and the third base 53 and the fourth base 57 are set at positions on the second bottom plate 2; then, the fourth bracket 17 is clamped and limited by a clamping block, so that the first bracket 9 above the fourth bracket 17 and the fourth base 57 is smoothly erected above the second bottom plate 2;

[0096] Next, the first rotating shaft 44 is started. The first rotating shaft and the first bracket are threadedly connected. The first rotating shaft and the first chuck are rotatably connected. After the first rotating shaft 44 reciprocates, the threaded connection between the first rotating shaft and the first bracket will make the first chuck reciprocate, so that the distance between the first chuck and the second chuck can be adjusted, and the installation space of the second bottom plate is limited. After the installation space of the second bottom plate is limited, the third bottom plate is fixed above the second bottom plate to achieve the purpose of the third bottom plate being erected.

[0097] At this time, the experimental unit is placed on the jacking end of the jacking device, and at the same time, the first insertion rod is inserted into the groove of the positioning cylinder, so that the experimental unit can be positioned on the upper surface of the third bottom plate by using the positioning cylinder and the first insertion rod;

[0098] Next, the top surface of the first plug rod is provided with an annular electromagnet, and the inner top surface of the positioning tube is provided with a magnetic ring; a trigger switch is also provided at the center of the upper top surface of the first plug rod, and when the trigger switch contacts the inner top surface of the positioning tube, the trigger switch is triggered, and when the trigger switch is triggered, the electromagnet is activated, and when the electromagnet is activated, it attracts the magnetic ring provided on the positioning tube;

[0099] Furthermore, during the process of positioning and adsorption by the magnet, the first insertion rod and the positioning cylinder will make the central axis of the first slot below the experimental unit and the first clamping device cooperate with each other; at the same time, the adsorption of the electromagnet and the magnetic ring makes the third partition plate and the fourth partition plate in the first slot be clamped by the clamping end of the clamping rod on the first clamping device; when clamping, the clamping gap between the two clamping rods 32 is inserted into the two side walls of the third partition plate and the fourth partition plate, and as the force of the spring is opposite to the force of the clamping end of the clamping rod 32, the clamping gap between the clamping rods is restrained and adjusted, so that the clamping end can be clamped by the action of the spring; at this time, combined with the first clamping device and the limit between the first insertion rod and the positioning cylinder, the experimental unit can be firmly fixed on the top surface of the third bottom plate;

[0100] In addition, in order to further improve its stability, a second bracket is also provided on the third panel, and a motor provided on the second bracket can be started simultaneously with the mutual triggering of the first insertion rod and the positioning cylinder, so that the experimental unit can be clamped by the clamping blocks on both sides of the experimental unit;

[0101] When the clamping block clamps the experimental unit, a conductive sheet is provided at the clamping position of the clamping block and the outer wall of the experimental unit, and the conductive sheet of the clamping block and the experimental unit can be connected to the power supply of the camera, so that the camera can be powered on and collect data above the experimental unit;

[0102] The second connecting rod on the first clamping device can abut against the trigger end of the triggering platform as the clamping rod is clamped, so that the triggering platform can start the valves of the sixth pipeline and the seventh pipeline; the sixth pipeline and the seventh pipeline are temperature regulating pipelines;

[0103] When the temperature and humidity sensor reaches a first preset value, the temperature-raising valve connected to the sixth pipeline is opened, and the temperature below the experimental unit is raised;

[0104] When the temperature and humidity sensor reaches the second preset value, the heating valve of the sixth pipeline is closed, and the cooling valve of the seventh pipeline is started to open, so that the cooling gas or liquid is transported to the seventh pipeline by the seventh pipeline.

[0105] In order to achieve temperature uniformity of the experimental unit, a temperature control pipe is also provided in the experimental unit, and the temperature control pipe includes a heating pipe and a cooling pipe respectively. The heating pipe is connected to the first discharge pipe, and the cooling pipe is connected to the second discharge pipe. The first discharge pipe and the second discharge pipe respectively realize temperature regulation of the experimental unit through the sixth pipe and the seventh pipe.

[0106] The first pipeline 4, the second pipeline 5, and the third pipeline 6 are used to feed materials to the first test unit, so that materials can be added to the first test unit according to the progress of the experiment during the experiment.

[0107] The initial setting of the lifting device is that the lifting end has the smallest size, so that the bottom surface of the experimental unit can smoothly match with the first clamping device, and the positioning cylinder and the first insertion rod can cooperate with each other;

[0108] When the experimental unit needs to be disassembled, the driving motor of the lifting device is started, and the lifting end of the lifting device will be pushed upward. The magnetic attraction force of the electromagnetic connection between the positioning cylinder and the first plug rod is smaller than the pushing force of the lifting device. Therefore, the lifting device can push the first plug rod and the positioning cylinder to disengage, thereby causing the electromagnet to disengage. After the electromagnet disengages, the electrical connection of the conductive sheet on the experimental unit can be cut off, thereby achieving the closing of the camera. At the same time, when the conductive sheet is powered off, the motor connected to the clamp block is started synchronously, and the purpose of the clamp block loosening the experimental unit is achieved.

[0109] When the clamping block clamps the experimental unit, a conductive sheet is provided at the clamping point of the clamping block and the outer wall of the experimental unit. The conductive sheet of the clamping block and the experimental unit can be connected to the power supply of the camera, so that the camera can be powered on and collect data above the experimental unit.

[0110] The fourth pipeline 7 is used to feed materials to the second test unit, so that materials can be added to the second test unit according to the progress of the experiment during the experiment.

[0111] The third test unit can generally be used as a comparative experimental example for experiments.

[0112] The first pipeline 4, the second pipeline 5, the third pipeline 6 and the fourth pipeline 7 are also connected to electromagnetic valves respectively, and the opening and closing of the electromagnetic valves can be activated by the connection of the conductive sheet, and the controller is used to realize the opening or closing of each electromagnetic valve in turn according to the progress of the experiment.

[0113] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A cell compatibility testing system for preparing bionic bone based on composite materials, characterized in that: include: An experimental platform, on which a plurality of experimental units (14) are arranged, each of the experimental units (14) being used to perform a cell compatibility experiment on the bionic bone; An information collection module, wherein the information detection module is used to collect information from the test units on the test bench; A controller, the controller is used to record the information collected by the information collection module for multiple times, and to compare the information collected for multiple times with the pre-stored information and obtain the cell compatibility experiment result; The experimental table comprises: an experimental unit (14), the experimental unit (14) being mounted on the upper surface of a third bottom plate (3) through a plurality of first clamping devices (18), the upper surface of the third bottom plate (3) being symmetrically provided with a plurality of first clamping devices (18), the first clamping device (18) comprising a second bracket (11), the second bracket (11) being a T-shaped structure, the lateral end of the T-shaped structure of the second bracket (11) being fixed on the upper surface of the third bottom plate (3), the vertical end of the second bracket (11) being provided with a motor, the motor being facing a side of the experimental unit (14), the output end of the motor being connected to a push rod (25), the end of the push rod (25) being away from the output end of the motor being connected to a clamp block (24) of an L structure, the L opening of the clamp block (24) being arranged downward; the side of the clamp block (24) close to the experimental unit (14) being used for abutting against the experimental unit (14); A first partition (15) and a second partition (21) are arranged in the experimental unit (14); the first partition (15) and the second partition (21) respectively isolate the experimental unit (14) to form a first experimental unit (20), a second experimental unit (22) and a third experimental unit (23); a first pipe (4), a second pipe (5), a third pipe (6) and a fourth pipe (7) are arranged at intervals on a side of the first experimental unit (20) away from the second experimental unit (22) and the third experimental unit (23); A third bracket (12) is arranged at intervals on one side of the second test unit (22) and the third test unit (23) away from the first test unit (20); the third bracket (12) is used to set up a first connecting rod (13); a camera is arranged on the lower surface of one end of the first connecting rod (13) away from the third bracket (12); the camera is used to collect video or image information in the experimental unit (14).

2. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 1, characterized in that: Also included is a terminal, which is used to output or display the cell compatibility experiment results obtained by the controller.

3. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 1, characterized in that: The information acquisition module is used to acquire osteoblast compatibility information of the bionic bone and transmit the compatibility information to the controller; The osteoblast compatibility information includes: cell proliferation effect detection information and alkaline phosphatase activity information.

4. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 1, characterized in that: The information collection module is used to collect cell proliferation effect detection information, and collects experimental information of multiple time periods for the cell proliferation effect detection information of each experimental unit (14); Furthermore, the information collection module is also used to collect alkaline phosphatase activity information, and collects experimental information of multiple time periods for the alkaline phosphatase activity information of each experimental unit (14).

5. The cell compatibility testing system for preparing bionic bone based on composite materials according to claim 1, characterized in that: The fourth pipe (7) penetrates through the side wall of the first test unit (20) and extends into the second test unit (22); a fifth pipe (26) is further provided on a side of the second test unit (22) away from the first test unit (20); fixing components are provided on both sides of the third bottom plate (3); the fixing components are used to fix the third bottom plate (3) on the experimental table; The fixing assembly comprises: a first bracket (9) and a fourth bracket (17), wherein the first bracket (9) is spaced apart on the second bottom plate (2) via a fourth base (57), and the fourth bracket (17) is spaced apart on the second bottom plate (2) via a third base (53), and a fourth bottom plate (55) is further arranged between the first bracket (9) and the fourth bracket (17), and the upper surface of the fourth bottom plate (55) is used for connecting to the third bottom plate (3); Fixed plates (56) are arranged at intervals below the fourth bottom plate (55), and the fixed plates (56) are used to connect the first chuck (10) and the second chuck (16); one side of the first chuck (10) is used to connect with the first bracket (9), and the other side of the first chuck (10) is used to clamp the third bottom plate (3); one side of the second chuck (16) is connected to the fourth bracket (17), and the other side of the second chuck (16) and the first chuck (10) cooperate with each other to clamp the third bottom plate (3); A shaft sleeve (8) is provided on a side of the first bracket (9) away from the first chuck (10), and a first rotating shaft (44) is provided inside the shaft sleeve (8). The first rotating shaft (44) passes through the first bracket (9) and is rotatably connected to the first chuck (10). The first rotating shaft (44) is used to adjust the gap between the first chuck (10) and the second chuck (16).

6. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 5, characterized in that: A plurality of clamping blocks (54) are arranged on the third base (53), and each clamping block (54) is used to clamp the fourth bracket (17) on the upper top surface of the third base (53); A control box (46) is also provided on one side of the third base (53), a controller is provided in the control box (46), and the controller is electrically connected to the camera; The third bottom plate (3) is also provided with a first clamping device (18), and a plurality of the first clamping devices (18) are provided and are spaced apart on the third bottom plate (3). A second base (40) is also provided between two adjacent first clamping devices (18), and a first insertion rod (39) is provided on the upper surface of the second base (40), and the first insertion rod (39) is used to cooperate with a positioning cylinder (42) on the lower surface of the test unit; A plurality of lifting devices (41) are arranged at intervals on one side of the first insertion rod (39) close to the center of the test unit, the fixed end of the lifting device (41) is connected to the third bottom plate (3), and the movable end of the lifting device (41) faces upward and abuts against the lower bottom surface of the test unit; The lower bottom surface of the experimental unit (14) is provided with a first notch (28), and fourth partitions (30) are symmetrically arranged at both ends of the first notch (28), and a plurality of third partitions (29) are arranged between the fourth partitions (30), and the fourth partitions (30) and the third partitions (29) are respectively used for clamping the clamping ends of the first clamping device (18) for clamping.

7. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 6, characterized in that: The first clamping device (18) comprises: a first base (38), the lower part of the first base (38) is mounted on the upper surface of the third bottom plate (3), a vertical plate (35) and a trigger platform (37) are arranged at intervals on the upper surface of the first base (38), a second hinge shaft (34) is arranged at intervals on one side of the vertical plate (35), a clamping rod (32) is hinged on each of the second hinge shafts (34), a spring (61) is arranged at one end of the clamping rod (32) close to the second hinge shaft (34), a side of one of the clamping rods (32) away from the vertical plate (35) is rotatably connected to the second connecting rod (31) through the first hinge shaft (33), and the other end of the second connecting rod (31) abuts against or releases the trigger end of the trigger platform (37) according to the clamping state of the two clamping rods (32); The second connecting rod (31) is also provided with a shift rod (36), the shift rod (36) extending from the second connecting rod (31) to the outer side wall of one of the clamping rods (32), and the shift rod (36) is located between the trigger platform (37) and the first hinge shaft (33); A first discharge pipe (59) and a second discharge pipe (60) are also arranged at intervals on the upper surface of the third bottom plate (3); the first discharge pipe (59) and the second discharge pipe (60) are respectively connected to the output ends of the drainage pipe (49); one end of the drainage pipe (49) away from the sixth pipe (47) and the seventh pipe (48) passes through the fourth bracket (17) and the second chuck (16), and extends to the inside of the third bottom plate (3).

8. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 7, characterized in that: The bottom surfaces of the third base (53) and the fourth base (57) are respectively mounted on the upper surface of the second base plate (2) via transverse slide rails, and the lower part of the second base plate (2) is movably mounted on the upper surface of the first base plate (1) via longitudinal slide rails; The second chuck (16) and the fourth bracket (17) are both provided with a penetrating pipeline channel (50), the pipeline channel (50) is used for the drainage pipe (49) to pass through, one end of the drainage pipe (49) is connected to the sixth pipeline (47) and the seventh pipeline (48); the other end of the drainage pipe (49) is respectively connected to the first discharge pipe (59) and the second discharge pipe (60); The sixth pipe (47) and the seventh pipe (48) are respectively arranged in the second notch (58) of the support rod (51); one end of the support rod (51) away from the second notch (58) is mounted in the L-notch of the second angle iron (52); one side of the second angle iron is fixed to the side wall of the third base (53); The opening edge of the experimental unit (14) is provided with a sealing edge, and the sealing edge is used to cooperate with the sealing cover and to seal the opening of the experimental unit (14); The outer wall of the shaft sleeve (8) is provided with a slide groove, and the slide groove is used to install one side of the first angle iron (43), and the other side of the first angle iron (43) is connected to the outer wall of the first bracket (9).

9. A cell compatibility testing system for preparing bionic bone based on composite materials as claimed in claim 8, characterized in that: Also includes the following methods: The triggering platform (37) is used to trigger a valve provided on the sixth pipeline (47) or the seventh pipeline (48); When the first clamping device (18) clamps the third base plate (3), the second connecting rod (31) on the first clamping device (18) triggers the triggering platform (37). That is, when the first clamping device (18) clamps the third partition plate (29) or the fourth partition plate (30), The trigger station (37) will start the valves connected to the sixth pipeline (47) and the seventh pipeline (48) according to the preset; During the experiment, the valves of the sixth pipeline (47) and the seventh pipeline (48) are activated according to the test environment, including: when the test temperature is lower than a first preset value, the valve of the sixth pipeline (47) is activated; when the test temperature is higher than a second preset value, the valve of the seventh pipeline (48) is activated.

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