A method, device, equipment and medium for detecting effective information of droplet-type fluorescence

Abstract

The present invention discloses a method, device, equipment and medium for detecting effective information of droplet-type fluorescence, the method comprising: passing the target droplet through the detection area, obtaining the amplitude data of the signal to be detected in the two detection channels of the sample; if the sum of the amplitude data of the signal to be detected in the two detection channels is greater than or equal to the preset threshold value at this time, obtaining the peak data of the signal to be detected in the two detection channels, and calculating the ratio between the two peak data; if the ratio between the two peak data is within the preset normal fluorescence signal ratio range at this time, recording the peak data of the signal to be detected in the two detection channels, the ratio between the two peak data, and the receiving time corresponding to the two peak data, and obtaining the characteristic value of the effective information. By adopting the embodiment of the present invention, a simple, low-cost and easy-to-operate algorithm is constructed to remove the interference signal collected by the detection system, thereby improving the precision and accuracy of the detection system.

Description

Technical Field

[0001] The present invention relates to the field of signal detection technology, and in particular to a method, device, equipment and medium for detecting effective information of droplet-type fluorescence. Background Art

[0002] With the development of microfluidics technology, droplet-type multi-channel fluorescence detection technology is widely used in the absolute quantitative detection of nucleic acid molecules, especially in the quantitative detection of infectious pathogens, tumor gene detection, reproductive health, animal diseases and other aspects. Droplet-type multi-channel fluorescence detection technology disperses the reaction reagents mixed with a variety of different fluorescent substances into hundreds of thousands of tiny droplets, usually called "oil-in-water", which are sucked into the liquid path through the injection needle, and then irradiated with laser or other light sources in the detection flow channel. The detection system collects the fluorescence emitted by the fluorescent substance and reflects the physical and chemical properties in the droplets according to the strength of the fluorescence signal. Since air or impurities are inevitably mixed in the sample, the reflected light of the air-liquid interface of the "oil-in-air" formed by the air in the detection flow channel, or the reflected light of the impurity particles and the liquid phase interface, the scattered light will also pass through the optical path, and thus be received by the detection system, and the air or impurity particles will be mistakenly identified as droplets. If there are impurities other than droplets in the sample, some impurities will also emit certain light signals and be detected by the system's detector, resulting in a large error in the detection result, reducing the accuracy and precision of the detection system.

[0003] At present, common methods for removing the influence of interference signals such as impurities or air signals include liquid path design, optical path design and algorithm elimination. Liquid path design removes impurities such as air from the source so that they do not pass through the detection channel, including setting up liquid level detection, liquid level following or bypassing air, etc.; optical path design can prevent impurity signals such as air from being received by the detection system by designing different optical paths and using different filters, etc.; and algorithm elimination is to distinguish valid signals from invalid abnormal interference signals by signal characteristics after the detection system receives the air signal.

[0004] Among the common methods of removing interference signals, liquid circuit design can remove interference signals from the source, but whether adding liquid level detection, following or liquid circuit bypass, it will add additional devices, increase the complexity and cost of the system; similarly, the optical system is very effective in removing interference signals, but the optical path design and optical components will increase the complexity and cost of the detection system. Algorithm elimination is to remove interference signals after the detection system receives the interference signal by interfering with the characteristics of the fluorescence signal, such as signal characteristics that indirectly reflect the structure of the size of the interfering substance. In the complex on-site detection process, the structural characteristics such as the size of the air signal or impurities fluctuate greatly, and there is a risk of identifying air or impurity signals as droplets. Summary of the invention

[0005] The embodiments of the present invention provide a droplet-type fluorescence effective information detection method, device, equipment and medium, which can accurately eliminate the air signal collected by the detection system under the conditions of low detection cost and without changing the complexity of the system.

[0006] A first aspect of an embodiment of the present invention provides a droplet-type fluorescence effective information detection method, comprising:

[0007] passing the target sample through the detection area;

[0008] Obtain the amplitude data of the signals to be detected in the two detection channels at this time;

[0009] If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to the preset threshold value, the peak data of the signals to be detected in the two detection channels are obtained, and the ratio between the two peak data is calculated;

[0010] If the ratio between the two peak data is within the normal fluorescence signal ratio range at this time, record the peak data of the signals to be detected in the two detection channels, the ratio between the two peak data, and the receiving times corresponding to the two peak data to obtain the characteristic value of the effective information; if the ratio between the two peak data is not within the normal fluorescence signal ratio range, do not record it.

[0011] In a possible implementation manner of the first aspect, the normal fluorescence signal ratio range is obtained through factory experiments.

[0012] In a possible implementation of the first aspect, a specific method for obtaining the normal fluorescence signal ratio range is:

[0013] Allow a preset amount of droplets to pass through the detection area, and obtain amplitude data of the signals to be detected in the two detection channels at this time;

[0014] If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to the preset threshold value, the peak data of the signals to be detected in the two detection channels are obtained, and the ratio between the two peak data is calculated;

[0015] A normal fluorescence signal ratio range is established according to the ratio range between the two peak data; the normal fluorescence signal ratio range may be one or more.

[0016] In a possible implementation of the first aspect, a specific method for obtaining the normal fluorescence signal ratio range is:

[0017] Allow the droplet to be detected to pass through the detection area, and obtain the amplitude data of the signal to be detected in the two detection channels at this time;

[0018] Plotting the amplitude data of the two detection channels of the droplet into a one-dimensional graph of the fluorescence signal or a two-dimensional graph of the fluorescence signal;

[0019] The normal droplet signal is divided by the one-dimensional or two-dimensional fluorescence signal graph of the two detection channels, and the fluorescence signal ratio range of the droplet to be tested in the two detection channels is calculated as the normal fluorescence signal ratio range; the normal fluorescence signal ratio range can be one or more.

[0020] A second aspect of an embodiment of the present invention provides a droplet-type fluorescence effective information detection device, comprising:

[0021] An amplitude acquisition module, used for acquiring amplitude data of the signals to be detected in the two detection channels when the target sample passes through the two detection channels;

[0022] A ratio calculation module, used for obtaining peak data of the signals to be detected in the two detection channels if the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to a preset threshold, and calculating the ratio between the two peak data;

[0023] The recording module is used to record the peak data of the signals to be detected in the two detection channels, the ratio between the two peak data, and the receiving times corresponding to the two peak data if the ratio between the two peak data is within the preset normal fluorescence signal ratio range, and obtain the characteristic value of the effective information. If the ratio between the two peak data is not within the normal fluorescence signal ratio range, no recording is performed.

[0024] In a possible implementation of the second aspect, the droplet-type fluorescence effective information detection device further includes:

[0025] A preset module is used to allow a preset amount of droplets to pass through the detection area; obtain the amplitude data of the signal to be detected in the two detection channels at this time; if the sum of the amplitude data of the signal to be detected in the two detection channels at this time is greater than or equal to the preset threshold, obtain the peak data of the signal to be detected in the two detection channels, and calculate the ratio between the two peak data; formulate a normal fluorescence signal ratio range according to the ratio range between the two peak data; the normal fluorescence signal ratio range can be one or more.

[0026] In a possible implementation of the second aspect, the droplet-type fluorescence effective information detection device further includes:

[0027] The post-setting module is used to make the droplet to be tested pass through the detection area, and obtain the amplitude data of the signal to be detected in the two detection channels at this time; plot the amplitude data of the two detection channels of the droplet into a one-dimensional graph of the fluorescence signal or a two-dimensional graph of the fluorescence signal; divide the normal droplet signal through the one-dimensional graph of the fluorescence signal or the two-dimensional graph of the fluorescence signal of the two detection channels, and calculate the fluorescence signal ratio range of the droplet to be tested in the two detection channels as the normal fluorescence signal ratio range; the normal fluorescence signal ratio range can be one or more.

[0028] A third aspect of an embodiment of the present invention provides a droplet-type fluorescence effective information detection device, characterized in that it includes a processor, a memory, and a computer program stored in the memory and configured to be executed by the processor, and when the processor executes the computer program, it implements the droplet-type fluorescence effective information detection method as described above.

[0029] A fourth aspect of an embodiment of the present invention provides a computer-readable storage medium, characterized in that the computer-readable storage medium includes a stored computer program, wherein when the computer program is running, the device where the computer-readable storage medium is located is controlled to execute the droplet-type fluorescence effective information detection method as described above.

[0030] Compared with the prior art, the droplet-type fluorescence effective information detection method, device, equipment and medium disclosed in the embodiments of the present invention use the fluorescence peak ratio of two detection channels as the screening basis, combined with the preset normal fluorescence signal ratio for screening, to avoid recording the fluorescence peak ratio, fluorescence peak value, and acceptance time corresponding to the interference signal that does not meet the requirements, and record the fluorescence peak ratio, fluorescence peak value, and acceptance time corresponding to the normal information as the characteristic values ​​of the effective information, thereby improving the detection method without changing the complexity of the original detection system, and improving the precision and accuracy of the detection of the detection system using the embodiments of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a schematic flow chart of a method for detecting effective information of a droplet-type fluorescence provided by an embodiment of the present invention;

[0032] Figure 2 is a diagram of the detection result of an embodiment of the present invention without using the droplet-type fluorescence effective information detection method;

[0033] Figure 3 This is a diagram of the detection results of an embodiment of the present invention using a droplet-type fluorescence effective information detection method.

[0034] Figure 4 is a schematic diagram of performing normal droplet selection calculation based on a one-dimensional graph of a fluorescent signal according to an embodiment of the present invention;

[0035] Figure 5It is a schematic diagram of performing normal droplet selection calculation based on a two-dimensional graph of fluorescence signals according to an embodiment of the present invention. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] See also Figure 1 , is a flow chart of a method for detecting effective information of a droplet-type fluorescence provided by an embodiment of the present invention, comprising:

[0038] S10, allowing the target sample to pass through the detection area.

[0039] S11, obtaining the amplitude data of the signals to be detected in the two detection channels at this time.

[0040] S12. If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to a preset threshold, obtain the peak data of the signals to be detected in the two detection channels, and calculate the ratio between the two peak data.

[0041] S13, if the ratio between the two peak data is within the preset normal fluorescence signal ratio range, record the peak data of the signals to be detected in the two detection channels, the ratio between the two peak data, and the receiving times corresponding to the two peak data, and obtain the characteristic value of the effective information. If the ratio between the two peak data is not within the normal fluorescence signal ratio range, do not record.

[0042] The target samples passing through the detection channel mainly include droplets, impurities, air and other interfering substances. The fluorescence actively emitted by the droplets as effective signals is emitted by different fluorescent substances contained in the droplets when stimulated by laser or other light sources in the detection flow channel. The color of the emitted fluorescence is determined by the molecular structure of the fluorescent substance, while its intensity is mainly determined by the concentration and luminous efficiency of the fluorescent substance. The fluorescence ratio of the droplets is relatively fixed.

[0043] The interference signal of "oil-in-gas" or solid-liquid interface formed by air or impurity particles as noise is mainly formed by passive reflection from the interface, scattering the light emitted by laser or other light sources, attenuated by different lenses in the optical path, and finally recognized by the optical receiving equipment. Its intensity is mainly determined by the material properties of the impurity itself, and its fluorescence signal ratio is often not equal to the fluorescence ratio of the object to be inspected, so it can be effectively distinguished by the difference in fluorescence ratio.

[0044] Since the fluorescence signal ratio of the normal sample in the two detection channels is stable in an instrument or detection system, in S12-S13, the embodiment of the present invention detects whether the ratio of the peak values ​​of the two detection channels is within the normal fluorescence signal ratio range. If the ratio between the two peak data is within the preset normal fluorescence signal ratio range, the peak data of the signal to be detected in the two detection channels, the ratio between the two peak data, and the receiving time corresponding to the two peak data are recorded to obtain the characteristic value of the effective information. If the ratio between the two peak data is not within the normal fluorescence signal ratio range, the obtained related data is discarded and not recorded.

[0045] like Figure 3 As shown, only when the ratio of the peak values ​​of the two detection channels is within the normal fluorescence signal ratio range, the ratio, the corresponding peak values ​​of the two detection channels and the corresponding peak reception time are recorded, and these three parameters are used as the characteristic values ​​of the effective signal. Figure 3 Only the effective signals of the droplets are recorded, and the related interference signals caused by air or foreign particles are not recorded. Figure 2 , if the S13 method is not adopted, the final detection result will contain interference signals.

[0046] It should be noted that the embodiment of the present invention records the ratio of the peak values ​​of the two detection channels within a preset range, the peak values ​​of the two detection channels and the corresponding peak reception times as characteristic values ​​of the effective signal formed by the droplets, and uses them as the detection results of the effective signal detection. The number of characteristic values ​​can be adjusted according to actual conditions.

[0047] Exemplarily, the normal fluorescence signal ratio range is obtained through factory experiments.

[0048] In practical applications, a software algorithm may be used to determine whether the ratio of the peak values ​​of the two detection channels is within the normal fluorescence signal ratio range, and the characteristic values ​​whose fluorescence ratios are not within the preset range may be deleted.

[0049] Exemplarily, a specific method for obtaining the normal fluorescence signal ratio range is:

[0050] A preset amount of droplets is passed through the detection area, and the amplitude data of the signals to be detected in the two detection channels are obtained at this time.

[0051] If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to the preset threshold, the peak data of the signals to be detected in the two detection channels are obtained, and the ratio between the two peak data is calculated.

[0052] A normal fluorescence signal ratio range is established according to the ratio range between the two peak data; the normal fluorescence signal ratio range may be one or more.

[0053] Exemplarily, another specific method for obtaining the normal fluorescence signal ratio range is:

[0054] The droplet to be detected is passed through the detection area, and the amplitude data of the signals to be detected in the two detection channels are obtained at this time.

[0055] The amplitude data of the two detection channels of the droplet are plotted into a one-dimensional graph of the fluorescence signal or a two-dimensional graph of the fluorescence signal.

[0056] The normal droplet signal is divided by a one-dimensional graph or a two-dimensional graph of the fluorescence signals of the two detection channels, and the ratio range of the fluorescence signals of a preset amount of droplets in the two detection channels is calculated as the normal fluorescence signal ratio range; the normal fluorescence signal ratio range can be one or more.

[0057] See also Figure 4 , Figure 4 A one-dimensional graph of the fluorescence signal is used to calculate the normal fluorescence signal ratio range:

[0058] In the one-dimensional graph of the droplet fluorescence signal, the normal droplets are selected by the upper threshold line and the lower threshold line. Then, the fluorescence signal ratio of the normal droplets is calculated, and the calculated fluorescence signal ratio range is the normal fluorescence signal ratio range used for judgment.

[0059] It should be noted that the one-dimensional graph here is only drawn based on the amplitude data of one detection channel. The actual ratio calculation also requires the one-dimensional graph drawn in combination with the amplitude data of another detection channel. The following method of using a two-dimensional graph to calculate the normal fluorescence signal ratio range is similar.

[0060] See also Figure 5 , Figure 5 A two-dimensional fluorescence signal graph is used to calculate the normal fluorescence signal ratio range:

[0061] In the two-dimensional graph of droplet fluorescence signals, a normal droplet is selected by box selection. Then the fluorescence signal ratio of the normal droplet is calculated, and the calculated fluorescence signal ratio range is the normal fluorescence signal ratio range used for judgment.

[0062] In the embodiment of the present invention, the liquid circuit structure does not need to be modified or added. By changing the liquid circuit sequence, the normal fluorescence signal ratio range is calculated by a preset or post-set method; after obtaining the fluorescence amplitude in the same way as in the previous section, the range interval of the ratio between the two peak data is judged, and the data that does not meet the conditions is eliminated. Generally speaking, multiple tests are performed, and the upper and lower limits of the normal fluorescence signal ratio range are set according to the ratios between multiple sets of peak data.

[0063] By automatically setting the normal fluorescence signal ratio range, the convenience of testing is further improved.

[0064] Compared with the prior art, the droplet-type fluorescence effective information detection method disclosed in the embodiment of the present invention uses the fluorescence peak ratio of two detection channels as the screening basis, combined with the preset normal fluorescence signal ratio for screening, to avoid recording the fluorescence peak ratio, fluorescence peak value, and acceptance time corresponding to the interference signal that does not meet the requirements, and records the fluorescence peak ratio, fluorescence peak value, and acceptance time corresponding to the effective information as the characteristic values ​​of the effective information, thereby improving the detection method without changing the complexity of the original detection system, and improving the precision and accuracy of the detection of the detection system using the embodiment of the present invention.

[0065] An embodiment of the present invention provides a droplet-type fluorescence effective information detection device, including: an amplitude acquisition module, a ratio calculation module and a recording module.

[0066] The amplitude acquisition module is used to acquire the amplitude data of the signals to be detected in the two detection channels when the target sample passes through the detection area.

[0067] The ratio calculation module is used to obtain the peak data of the signals to be detected in the two detection channels if the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to a preset threshold, and calculate the ratio between the two peak data.

[0068] The recording module is used to record the peak data of the signals to be detected in the two detection channels, the ratio between the two peak data, and the receiving times corresponding to the two peak data if the ratio between the two peak data is within the normal fluorescence signal ratio range, and obtain the characteristic value of the effective information. If the ratio between the two peak data is not within the normal fluorescence signal ratio range, no recording is performed.

[0069] Exemplarily, the droplet-type fluorescence effective information detection device further includes:

[0070] A preset module is used to allow a preset amount of droplets to pass through the detection area; obtain the amplitude data of the signal to be detected in the two detection channels at this time; if the sum of the amplitude data of the signal to be detected in the two detection channels at this time is greater than or equal to the preset threshold, obtain the peak data of the signal to be detected in the two detection channels, and calculate the ratio between the two peak data; formulate a normal fluorescence signal ratio range according to the ratio range between the two peak data; the normal fluorescence signal ratio range can be one or more.

[0071] Exemplarily, the droplet-type fluorescence effective information detection device further includes:

[0072] The post-setting module is used to allow a preset amount of droplets to pass through the detection area and obtain the amplitude data of the signals to be detected in the two detection channels at this time; plot the amplitude data of the two detection channels of the droplets into a one-dimensional graph of the fluorescence signal or a two-dimensional graph of the fluorescence signal; divide the normal droplet signal through the one-dimensional graph of the fluorescence signal or the two-dimensional graph of the fluorescence signal of the two detection channels, and calculate the fluorescence signal ratio range of the preset amount of droplets in the two detection channels as the normal fluorescence signal ratio range; the normal fluorescence signal ratio range can be one or more.

[0073] Those skilled in the art can clearly understand that, for the convenience and brevity of description, the specific working process of the device described above can refer to the corresponding process in the aforementioned method embodiment, and will not be described again here.

[0074] An embodiment of the present invention provides a droplet-type fluorescence effective information detection device, comprising a processor, a memory, and a computer program stored in the memory and configured to be executed by the processor, wherein the processor implements the droplet-type fluorescence effective information detection method as described above when executing the computer program.

[0075] An embodiment of the present invention provides a computer-readable storage medium, which includes a stored computer program, wherein when the computer program is executed, the device where the computer-readable storage medium is located is controlled to execute the droplet-type fluorescence effective information detection method as described above.

[0076] Exemplarily, the computer program may be divided into one or more modules / units, which are stored in the memory and executed by the processor to complete the present invention. The one or more modules / units may be a series of computer program instruction segments capable of completing specific functions, which are used to describe the execution process of the computer program in the droplet-type fluorescence effective information detection device / equipment. For example, the computer program may be divided into an amplitude acquisition module, a ratio calculation module, and a recording module.

[0077] The microdroplet type fluorescence effective information detection device can be a computing integrated device such as a desktop computer, a cloud detection system, etc. The microdroplet type fluorescence effective information detection device can include, but is not limited to, a processor and a memory. Those skilled in the art can understand that the schematic diagram is only an example of a microdroplet type fluorescence effective information detection device and does not constitute a limitation on the microdroplet type fluorescence effective information detection device. It can include more or less components than shown in the figure, or combine certain components, or different components. For example, the microdroplet type fluorescence effective information detection device can also include input and output devices, network access devices, buses, etc.

[0078] The processor may be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor may be a microprocessor or any conventional processor, etc. The processor is the control center of the microdroplet fluorescence effective information detection device, and uses various interfaces and lines to connect various parts of the entire microdroplet fluorescence effective information detection device.

[0079] The memory can be used to store the computer program and / or module, and the processor realizes various functions of the droplet-type fluorescence effective information detection device by running or executing the computer program and / or module stored in the memory, and calling the data stored in the memory. The memory can mainly include a program storage area and a data storage area, wherein the program storage area can store an operating system, an application required for at least one function (such as a sound playback function, an image playback function, etc.), etc.; the data storage area can store data created according to the use of the mobile phone (such as audio data, a phone book, etc.), etc. In addition, the memory can include a high-speed random access memory, and can also include a non-volatile memory, such as a hard disk, a memory, a plug-in hard disk, a smart memory card (Smart Media Card, SMC), a secure digital (SecureDigital, SD) card, a flash card (Flash Card), at least one disk storage device, a flash memory device, or other volatile solid-state storage devices.

[0080] Wherein, if the module / unit integrated in the droplet-type fluorescence effective information detection device is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on such an understanding, the present invention implements all or part of the processes in the above-mentioned embodiment method, and can also be completed by instructing the relevant hardware through a computer program. The computer program can be stored in a computer-readable storage medium, and the computer program can implement the steps of the above-mentioned various method embodiments when executed by the processor. Wherein, the computer program includes computer program code, and the computer program code can be in source code form, object code form, executable file or some intermediate form, etc. The computer-readable medium may include: any entity or device capable of carrying the computer program code, recording medium, U disk, mobile hard disk, disk, optical disk, computer memory, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), electrical carrier signal, telecommunication signal and software distribution medium, etc. It should be noted that the content contained in the computer-readable medium can be appropriately increased or decreased according to the requirements of legislation and patent practice in the jurisdiction. For example, in some jurisdictions, according to legislation and patent practice, computer-readable media does not include electrical carrier signals and telecommunication signals.

[0081] It should be noted that the device embodiments described above are merely schematic, wherein the units described as separate components may or may not be physically separated, and the components displayed as units may or may not be physical units, that is, they may be located in one place, or they may be distributed on multiple network units. Some or all of the modules may be selected according to actual needs to achieve the purpose of the scheme of this embodiment. In addition, in the accompanying drawings of the device embodiments provided by the present invention, the connection relationship between the modules indicates that there is a communication connection between them, which may be specifically implemented as one or more communication buses or signal lines. A person of ordinary skill in the art may understand and implement it without paying any creative effort.

[0082] The above is a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the principle of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A droplet-type fluorescence effective information detection method, characterized in that: include: passing the target sample through the detection area; Obtain the amplitude data of the signals to be detected in the two detection channels at this time; If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to the preset threshold value, the peak data of the signals to be detected in the two detection channels are obtained, and the ratio between the two peak data is calculated; If the ratio between the two peak data is within the normal fluorescence signal ratio range, the peak data of the signals to be detected in the two detection channels, the ratio between the two peak data, and the receiving times corresponding to the two peak data are recorded to obtain the characteristic value of the effective information; If the ratio between the two peak data is not within the normal fluorescence signal ratio range, no recording is performed.

2. The method for detecting effective information of droplet-type fluorescence according to claim 1, characterized in that: The normal fluorescence signal ratio range is obtained through a preset experiment, or is calculated by dividing the normal signal after testing the droplet to be tested.

3. The droplet-type fluorescence effective information detection method according to claim 1, characterized in that: The specific method for obtaining the normal fluorescence signal ratio range is: Allow a preset amount of droplets to pass through the detection area, and obtain amplitude data of the signals to be detected in the two detection channels at this time; If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to the preset threshold value, the peak data of the signals to be detected in the two detection channels are obtained, and the ratio between the two peak data is calculated; A normal fluorescence signal ratio range is established according to the ratio range between the two peak data; the normal fluorescence signal ratio range may be one or more.

4. The method for detecting effective information of droplet-type fluorescence according to claim 1, characterized in that: The specific method for obtaining the normal fluorescence signal ratio range is: Allow the droplet to be detected to pass through the detection area, and obtain the amplitude data of the signal to be detected in the two detection channels at this time; Plotting the amplitude data of the two detection channels of the droplet into a one-dimensional graph of the fluorescence signal or a two-dimensional graph of the fluorescence signal; The normal droplet signal is divided by the one-dimensional or two-dimensional fluorescence signal graph of the two detection channels, and the fluorescence signal ratio range of the droplet to be tested in the two detection channels is calculated as the normal fluorescence signal ratio range; the normal fluorescence signal ratio range can be one or more.

5. A droplet-type fluorescence effective information detection device, characterized in that: include: The amplitude acquisition module is used to acquire the amplitude data of the signals to be detected in the two detection channels when the target sample passes through the detection area; A ratio calculation module, used for obtaining peak data of the signals to be detected in the two detection channels if the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to a preset threshold, and calculating the ratio between the two peak data; A recording module, for recording the peak data of the signals to be detected in the two detection channels, the ratio between the two peak data, and the receiving times corresponding to the two peak data, if the ratio between the two peak data is within the normal fluorescence signal ratio range, to obtain a characteristic value of effective information; If the ratio between the two peak data is not within the normal fluorescence signal ratio range, no recording is performed.

6. The microdroplet type fluorescence effective information detection device as claimed in claim 5, characterized in that: Also includes: A preset module, used to allow a preset amount of droplets to pass through the detection area; Obtain the amplitude data of the signals to be detected in the two detection channels at this time; If the sum of the amplitude data of the signals to be detected in the two detection channels is greater than or equal to the preset threshold value, the peak data of the signals to be detected in the two detection channels are obtained, and the ratio between the two peak data is calculated; The normal fluorescence signal ratio range may be one or more.

7. The microdroplet type fluorescence effective information detection device according to claim 5, characterized in that: Also includes: A post-installation module is used to make the droplet to be detected pass through the detection area and obtain the amplitude data of the signal to be detected in the two detection channels at this time; The amplitude data of the two detection channels of the droplet are plotted into a one-dimensional graph of fluorescence signals or a two-dimensional graph of fluorescence signals; the normal droplet signal is divided by the one-dimensional graph of fluorescence signals of the two detection channels or the two-dimensional graph of fluorescence signals, and the ratio range of the fluorescence signals of the droplet to be tested in the two detection channels is calculated as the normal fluorescence signal ratio range; the normal fluorescence signal ratio range can be one or more.

8. A microdroplet type fluorescence effective information detection device, characterized in that: The method comprises a processor, a memory, and a computer program stored in the memory and configured to be executed by the processor, wherein the processor implements the droplet-type fluorescence effective information detection method as described in any one of claims 1 to 4 when executing the computer program.

9. A computer-readable storage medium, characterized in that: The computer-readable storage medium includes a stored computer program, wherein when the computer program is executed, the device where the computer-readable storage medium is located is controlled to execute the droplet-type fluorescence effective information detection method according to any one of claims 1 to 4.

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