A heating device and nucleic acid extractor

Abstract

The application relates to a heating device and a nucleic acid extractor, and relates to the technical field of molecular biology. The heating device comprises a heating main body provided with a reagent box fixing position on the surface. The heating main body comprises a first heating body and a second heating body which are arranged in a detachable mode in an up-down mode. A plurality of heating units are arranged on the upper surfaces of the first heating body and the second heating body. The heating units on the second heating body pass through the first heating body upwards to the upper surface of the first heating body, thereby supplementing the heating positions on the upper surface of the first heating body. The upper surface of the first heating body is provided with three reagent box fixing positions. The back surface of the first heating body corresponding to the three reagent box fixing positions is provided with three first electric heating films. The back surface of the second heating body corresponding to the three reagent box fixing positions is provided with three second electric heating films. The application solves the problem that the existing 32-person universal nucleic acid extractor cannot heat 8 columns of 32-person nucleic acid extraction reagent boxes in one-step elution.

Description

Technical Field

[0001] The present application relates to the field of molecular biotechnology, and in particular to a heating device and a nucleic acid extraction instrument. Background Art

[0002] Nucleic acid extractors are instruments used with nucleic acid extraction reagents to extract nucleic acid from samples. They are widely used in a variety of fields, including disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry, and molecular biology research.

[0003] Nucleic acid extraction includes the following basic steps: 1. Lysis: Add lysis solution to the sample, and use mechanical movement and heating to achieve mixing and sufficient reaction of the reaction solution, so that the cells are lysed and the nucleic acids are released; 2. Adsorption: Add magnetic beads to the sample lysis solution and mix thoroughly. Magnetic beads have a strong affinity for nucleic acids under high salt and low pH conditions to adsorb nucleic acids, and separate the magnetic beads from the lysis solution under the action of an external magnetic field; 3. Washing: Transfer the magnetic beads adsorbed with nucleic acids to a new washing solution and mix thoroughly to wash away impurities, and then separate the magnetic beads from the washing solution under the action of an external magnetic field; 4. Elution: Transfer the magnetic beads to the eluent, remove the external magnetic field, and mix the magnetic beads and the eluent thoroughly. The bound nucleic acids can be separated from the magnetic beads, and then the magnetic beads and the eluent are separated under the action of an external magnetic field to obtain purified nucleic acids.

[0004] In the above-mentioned extraction steps, the lysis and elution steps require heating. Existing nucleic acid extractors are usually equipped with a heating device. When performing nucleic acid extraction, the reagent kit is placed in the reagent compartment of the nucleic acid extractor, and the heating device is placed at the position where the lysis solution and elution solution are contained in the reagent kit for heating.

[0005] The existing nucleic acid extraction instrument's heating system and fixed lysis and elution heating positions are only suitable for the universal two-step elution method, a 16-sample nucleic acid extraction kit. This kit has four heating positions: the 1st and 7th positions correspond to the lysis positions, and the 6th and 12th positions correspond to the elution positions. Each heating position uses an electric heating rod. For example, the existing 32-sample throughput nucleic acid extraction instrument can accommodate two universal two-step elution 16-sample extraction kits side by side. These kits have eight heating positions and require eight electric heating rods for heating.

[0006] However, existing one-step elution nucleic acid extraction kits have emerged, capable of handling up to 32 samples per patient. These kits feature eight heating stations: cleavage stations in rows 1, 4, 7, and 11, and elution stations in rows 3, 6, 9, and 12. For a 32-sample throughput nucleic acid extraction instrument, the heating system using four heating stations is no longer suitable for a one-step elution nucleic acid extraction kit with eight heating stations and handling 32 samples per patient.

[0007] For a 32-person nucleic acid extraction kit using the one-step elution method, eight heating methods are required. If the traditional electric heating rod method is used, eight electric heating rods will be needed within the same nucleic acid extraction kit plate well space, which will undoubtedly greatly increase the difficulty of instrument design, increase the complexity of instrument assembly, and increase the instrument cost. Summary of the Invention

[0008] The purpose of this application is to provide a heating device and a nucleic acid extractor, which solves the problem in the prior art that the 32-person universal nucleic acid extractor is not suitable for heating the 8 columns of the 32-person nucleic acid extraction kit using the one-step elution method.

[0009] In the first aspect, the present application provides a heating device adopting the following technical solution:

[0010] A heating device includes a heating body with a reagent box fixing position on its surface, the heating body includes a first heating body and a second heating body that are detachably arranged relative to each other up and down, the upper surfaces of the first heating body and the second heating body are both provided with multiple rows of heating units, the heating units on the second heating body pass upward through the first heating body to the upper surface of the first heating body, and supplement the heating positions on the upper surface of the first heating body.

[0011] By adopting the above technical solution, two heating bodies are set up, and the heating unit of the second heating body can pass through the first heating body and be used in conjunction with the heating unit on the surface of the first heating body. This increases the number of times the heating body heats the reagent kit on the reagent kit fixed position, improves the efficiency of the heating device, and can meet the 8-column heating requirements of the 32-person nucleic acid extraction kit using the one-step elution method.

[0012] Optionally, the upper surface of the first heating body has three reagent box fixing positions, the back of the first heating body corresponding to the three reagent box fixing positions has three first electric heating films, and the back of the second heating body corresponding to the three reagent box fixing positions has three second electric heating films.

[0013] By adopting the above technical solution, three reagent kit fixing positions are provided on the first heating element. Each reagent kit fixing position can heat 32 nucleic acid extraction kits for each person, thereby achieving 96 nucleic acid extraction kits for each person, that is, three 32 nucleic acid extraction kits for the one-step elution method can be heated simultaneously, greatly improving the efficiency and speed of nucleic acid extraction. Both heating elements are heated by electric heating membranes. The electric heating membranes are thin, occupy little space, cover a large area, and have good heating effects. Integrating the electric heating membranes into the two plate-like heating elements can reduce the thickness of the entire heating device, making the heating device smaller, easier to install, covering a large area, and improving work efficiency.

[0014] Optionally, each reagent kit fixing position on the first heating body is provided with a first row of lysis heating tanks, a fourth row of lysis heating tanks, a seventh row of lysis heating tanks and a tenth row of lysis heating tanks respectively;

[0015] Below each reagent kit fixing position on the second heating body are respectively provided with the 3rd column elution heating slot, the 6th column elution heating slot, the 9th column elution heating slot and the 12th column elution heating slot.

[0016] By adopting the above technical solution, the 1st, 4th, 7th and 10th columns in the fixed position of the reagent kit are set as lysis heating tanks, corresponding to the first lysis heating step in the nucleic acid extraction step, and the 3rd, 6th, 9th and 12th columns in the fixed position of the reagent kit are set as elution heating tanks, corresponding to the third elution heating step in the nucleic acid extraction step, so that the entire heating device can carry out multiple steps and multiple groups at the same time, and the efficiency of nucleic acid extraction is significantly improved.

[0017] Optionally, the first heating body is provided with clearance holes at the 3rd, 6th, 9th and 12th columns of each reagent box fixing position, respectively, and the clearance holes respectively accommodate the 3rd column elution heating tank, the 6th column elution heating tank, the 9th column elution heating tank and the 12th column elution heating tank on the second heating body.

[0018] By adopting the above technical solution, the 3rd, 6th, 9th and 12th columns on the first heating body are provided with clearance holes, which can allow the 3rd, 6th, 9th and 12th columns of elution heating grooves on the second heating body to pass through. The two heating bodies can heat the reagent kit in the fixed position of the reagent kit at the same time, and the two heating bodies are separated in structure and can be controlled separately. The heating functions do not affect each other, and can meet the different temperature requirements in the lysis and elution steps.

[0019] Optionally, the protruding height of the elution heating groove on the second heating body is greater than the protruding height of the pyrolysis heating groove on the first heating body. When the first heating body and the second heating body are connected, the surface height of the pyrolysis heating groove is consistent with the surface height of the elution heating groove.

[0020] By adopting the above technical solution, after the heating device is assembled, the lysis heating tank and the elution heating tank are at the same height, so that the heights of the reagent kits on the two heating tanks are also the same, the reagent kit placement rack can be placed stably, and the movement of the reagent kit is smoother.

[0021] Optionally, there are heat dissipation holes at the 1st column, 4th column, 7th column and 10th column positions of each reagent kit fixing position on the second heating element.

[0022] By adopting the above technical solution, the excess heat on the first electric heating film can be dissipated through the heat dissipation holes, alleviating the situation where the temperature of the first heating body is too high due to the obstruction of the second heating body, thereby protecting the structural safety of the first heating body and the second heating body and extending the service life of the entire heating device.

[0023] Optionally, the first heating body is provided with a first heat preservation device on the back side corresponding to each reagent box fixing position, and the first heat preservation device is connected to the first electric heating film in a one-to-one correspondence to control the power on or off of the first electric heating film;

[0024] The second heating body is provided with a second heat preservation device on the back side corresponding to each reagent box fixing position. The second heat preservation device is connected to the second electric heating film in a one-to-one correspondence to control the power on or off of the second electric heating film.

[0025] By adopting the above technical solution, each electric heating film is equipped with a thermal insulation device, which is used to regulate the working state of the electric heating film, thereby achieving single-film single control and more precise control. If one electric heating film is damaged, it will not affect the operation of other electric heating films, and the maintenance and replacement costs of the equipment are also lower.

[0026] Optionally, the thermal insulation device includes a temperature sensor and a controller, and the controller sets an upper temperature limit;

[0027] When the temperature sensor senses that the temperature of the first heating element and / or the second heating element exceeds the upper temperature limit, the controller controls the electric heating film to cut off the circuit;

[0028] When the temperature sensor senses that the temperature of the first heating element and / or the second heating element drops below the upper temperature limit, the controller controls the electric heating film to be energized.

[0029] By adopting the above technical solution, the temperature sensor senses the problem of the two heating elements, and then compares it with the temperature upper limit value in the controller to regulate the state of the electric heating membrane to ensure the required temperature for nucleic acid extraction.

[0030] Optionally, a first temperature measuring probe is provided on each of the three first electric heating films; and a second temperature measuring probe is provided on each of the three second electric heating films.

[0031] By adopting the above technical solution, the temperature measuring probe detects the temperature of the two heating tanks through the heating body, which can more accurately know the temperature of the heating tank and facilitate accurate control of the required temperature for nucleic acid extraction.

[0032] In a second aspect, the present application provides a nucleic acid extraction instrument that adopts the following technical solution:

[0033] A nucleic acid extractor comprises a heating device.

[0034] In summary, this application includes at least one of the following beneficial technical effects:

[0035] 1. The heating device of the present application is fixed one above and one below the other by a first heating body and a second heating body, and three reagent box fixing positions are set on the first heating body. Four rows of lysis heating tanks and four rows of elution heating tanks are respectively set according to the lysis and elution positions of the reagent box, with a total of eight rows of heating tanks. Each of the four rows of lysis heating tanks and the four rows of elution heating tanks is heated and controlled by an electric heating film, so that the heating device can match the heating requirements of the lysis and elution steps of the 32-person nucleic acid extraction kit of the one-step elution method. The lysis and elution are heated in steps, which improves the heating efficiency, reduces heat radiation and excessive volatilization of reagents, ensures the stability of reagent concentration, and improves the efficiency and quality of nucleic acid extraction.

[0036] 2. The nucleic acid extraction instrument heating device of the present invention can achieve 96 servings, that is, 32 servings of nucleic acid extraction kits for three one-step elution methods, and heat them simultaneously. When the volume of the nucleic acid extraction instrument is similar to that of the 32-serving nucleic acid extraction instrument for two-step elution methods, the throughput is 96 servings, which is increased by 3 times, greatly improving the efficiency and speed of nucleic acid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 The three-dimensional heating device of the embodiment of the present application Figure 1 ;

[0038] Figure 2 This is a front view of a heating device according to an embodiment of the present application;

[0039] Figure 3 The three-dimensional heating device of the embodiment of the present application Figure 2 ;

[0040] Figure 4 The first heating body in the embodiment of the present application is a three-dimensional Figure 1 ;

[0041] Figure 5 The first heating body in the embodiment of the present application is a three-dimensional Figure 2 ;

[0042] Figure 6 This is a three-dimensional diagram of three first electric heating films below the first heating body in the embodiment of the present application;

[0043] Figure 7 The third embodiment of the present invention is the second heating body Figure 1 ;

[0044] Figure 8 The third embodiment of the present invention is the second heating body Figure 2 ;

[0045] Figure 9 This is a three-dimensional diagram of three second electric heating films below the second heating body in an embodiment of the present application.

[0046] In the figure, 10, heating body; 20, first heating body; 21, 22, 23, 24, cracking heating tank; 25, 26, 27, 28, give way hole; 30, second heating body; 31, 32, 33, 34, elution heating tank; 35, 36, 37, 38, heat dissipation hole; 39, connecting column; 40, first thermal insulation system; 41, 42, 43, first thermal insulation device; 50, first heat source; 51, 52, 53, first electric heating film; 60, second thermal insulation system; 61, 62, 63, second thermal insulation device; 70, second heat source; 71, 72, 73, second electric heating film; 81, 82, 83, first temperature measuring probe; 91, 92, 93, second temperature measuring probe. DETAILED DESCRIPTION

[0047] The following is combined with Figure 1 -Attached Figure 9 , further details of this application are given.

[0048] This application proposes a heating device, referring to Figure 1 and 2 The heating device is mainly composed of a heating body 10 and a reagent box fixing position. The heating body 10 includes two structures, a first heating body 20 and a second heating body 30, which are detachably connected together in the form of the first heating body 20 on the top and the second heating body 30 on the bottom. In this embodiment, the first heating body 20 and the second heating body 30 are both rectangular plate structures with good thermal conductivity. The two are arranged in parallel with a gap between them.

[0049] Three reagent kit fixing positions are provided on the upper surface of the first heating body 20, and the three reagent kit fixing positions are limited by four parallel baffles, which are installed on the surface of the first heating body 20. Multiple rows of heating units are provided on the upper surfaces of the first heating body 20 and the second heating body 30 corresponding to each reagent kit fixing position. Holes and slots are opened on the first heating body 20 so that the heating units on the second heating body 30 can pass through the first heating body 20 upward and protrude to the upper surface of the first heating body 20, so that there are more heating positions in each reagent kit fixing position.

[0050] Reference Figure 3A first heat source 50 is connected to the lower surface of the first heating body 20 at a position corresponding to the reagent chamber fixing position. The first heat source 50 heats the heating unit on the first heating body 20. In this embodiment, the width of the first heating body 20 is greater than the width of the second heating body 30. A first heat preservation system 40 is connected to the lower surface of the first heating body 20 at a portion exceeding the width of the second heating body 30. The first heat preservation system 40 is electrically connected to the first heat source 50 and is used to control the power on and off of the first heat source 50. A second heat source 70 is connected to the lower surface of the second heating body 30 at a position corresponding to the reagent chamber fixing position. The second heat source 70 heats the heating unit on the second heating body 30. A second heat preservation system 60 is connected to the lower surface of the second heating body 30. The second heat preservation system 60 is electrically connected to the second heat source 70 and is used to control the power on and off of the second heat source 70.

[0051] Reference Figure 4 The heating unit on the first heating body 20 adopts the 1st column lysis heating tank 21, the 4th column lysis heating tank 22, the 7th column lysis heating tank 23 and the 10th column lysis heating tank 24, which are arranged inside each reagent box fixing position, with a total of four columns, corresponding to the lysis steps in the nucleic acid extraction step. Each column of heating tanks is provided with eight separate single slots. The lysis heating tanks 21, 22, 23, and 24 in this embodiment adopt a solid structure with surface grooves, and therefore include a solid part, which is fixed to the upper surface of the first heating body 20. The first heating body 20 is provided with clearance holes 25, 26, 27, and 28 at the 3rd column, 6th column, 9th column and 12th column positions of each reagent box fixing position, respectively. The clearance holes 25, 26, 27, and 28 have the same shape and are larger in length and width than the lysis heating tanks 21, 22, 23, and 24.

[0052] Reference Figure 4 and 5 The first heat source 50 includes three first electric heating films 51, 52, and 53 connected to the back of the first heating body 20 corresponding to the three reagent box fixing positions. The length and width of the first electric heating films 51, 52, and 53 are adapted to the length and width of the reagent box fixing positions. The first electric heating films 51, 52, and 53 are also provided with strip holes at the positions corresponding to the clearance holes 25, 26, 27, and 28. The shape of the strip holes is the same as that of the clearance holes 25, 26, 27, and 28. The first temperature preservation system 40 includes three first temperature preservation devices 41, 42, and 43 connected to the back of the first heating body 20 corresponding to the three reagent box fixing positions. The first temperature preservation devices 41, 42, and 43 are staggered at the positions of the first electric heating films 51, 52, and 53 and are connected to the back of the first heating body 20. The first temperature preservation devices 41, 42, and 43 are connected to the first electric heating films 51, 52, and 53 in a one-to-one manner. The first temperature preservation devices 41, 42, and 43 control the power on or off of the first electric heating films 51, 52, and 53.

[0053] Specifically, the first thermal insulation devices 41, 42, and 43 all include a temperature sensor and a controller, and the controller sets the temperature upper limit; when the temperature sensor senses that the temperature of the position corresponding to the reagent box fixed position on the first heating element 20 exceeds the temperature upper limit, the controller controls the electric heating film at the corresponding position to disconnect and stop heating; when the temperature sensor senses that the temperature of the position corresponding to the reagent box fixed position on the first heating element 20 drops below the temperature upper limit, the controller controls the electric heating film at the corresponding position to energize and continue heating.

[0054] Reference Figure 4 and 6 The first temperature preservation system 40 also includes first temperature measuring probes 81, 82, and 83 respectively arranged on the three first electric heating films 51, 52, and 53. The first temperature measuring probes 81, 82, and 83 are arranged at a position close to one of the cracking heating tanks 21, 22, 23, and 24, and indirectly measure the temperature of the cracking heating tanks 21, 22, 23, and 24 through the first heating body 20.

[0055] Reference Figure 7 The heating unit on the second heating body 30 adopts the 3rd column elution heating tank 31, the 6th column elution heating tank 32, the 9th column elution heating tank 33 and the 12th column elution heating tank 34 set inside each reagent box fixing position, a total of four columns, corresponding to the third step elution heating in the nucleic acid extraction step, and each column of heating tank is provided with eight separate single tanks. The elution heating tanks 31, 32, 33, and 34 in this embodiment also adopt a physical structure with surface grooves, and the physical structure is fixed on the upper surface of the second heating body 30.

[0056] The second heating body 30 is provided with heat dissipation holes 35, 36, 37, 38 at the 1st, 4th, 7th and 10th columns of each reagent box fixing position, respectively. The heat dissipation holes 35, 36, 37, 38 are arranged as long strip holes parallel to the elution heating grooves 31, 32, 33, 34, and the length and width correspond to the length and width of the elution heating grooves 31, 32, 33, 34.

[0057] Reference Figure 7 and 8The second heat source 70 includes three second electric heating films 71, 72, and 73 connected to the back of the second heating body 30 corresponding to the three reagent box fixing positions. The length and width of the second electric heating films 71, 72, and 73 are adapted to the length and width of the reagent box fixing positions. The second electric heating films 71, 72, and 73 are also provided with strip holes at positions corresponding to the heat dissipation holes 35, 36, 37, and 38. The shape of the strip holes is the same as that of the heat dissipation holes 35, 36, 37, and 38. The second temperature preservation system 60 includes three second temperature preservation devices 61, 62, and 63 connected to the back of the second heating body 30 corresponding to the three reagent box fixing positions. The second temperature preservation devices 61, 62, and 63 are staggered at the positions of the second electric heating films 71, 72, and 73 and are connected to the back of the second heating body 30. The second temperature preservation devices 61, 62, and 63 are connected to the second electric heating films 71, 72, and 73 one by one. The second temperature preservation devices 61, 62, and 63 respectively control the power on or off of the second electric heating films 71, 72, and 73.

[0058] The second thermal insulation devices 61, 62, and 63 also include a temperature sensor and a controller, and the controller sets a temperature upper limit; when the temperature sensor senses that the temperature of the position corresponding to the reagent box fixing position on the second heating body 30 exceeds the temperature upper limit, the controller controls the electric heating film at the corresponding position to disconnect and stop heating; when the temperature sensor senses that the temperature of the position corresponding to the reagent box fixing position on the second heating body 30 drops below the temperature upper limit, the controller controls the electric heating film at the corresponding position to energize and continue heating.

[0059] Reference Figure 7 and 9 Similarly, the second temperature maintenance system 60 also includes second temperature measuring probes 91, 92, and 93 respectively arranged on the three second electric heating films 71, 72, and 73. The second temperature measuring probes 91, 92, and 93 are arranged at a position close to one of the elution heating tanks 31, 32, 33, and 34, and indirectly measure the temperature of the elution heating tanks 31, 32, 33, and 34 through the second heating body 30.

[0060] Reference Figure 2 、 4 and 7. A plurality of circular holes are provided on the edge of the first heating body 20, and a plurality of protruding connecting columns 39 are connected to the edge of the upper surface of the second heating body 30. A threaded hole is provided in the center of the connecting column. When the first heating body 20 and the second heating body 30 are connected relative to each other up and down, the circular holes on the first heating body 20 and the threaded holes on the connecting column 39 are relative to each other up and down. The first heating body 20 and the second heating body 30 are installed and fixed by means of bolts passing through the circular holes and threadedly connected to the threaded holes. The height of the connecting column 39 limits the distance between the first heating body 20 and the second heating body 30.

[0061] When the first heating body 20 and the second heating body 30 are assembled, the clearance holes 25, 26, 27, 28 are opposite to the elution heating grooves 31, 32, 33, 34 in upper and lower positions, the cracking heating grooves 21, 22, 23, 24 are opposite to the heat dissipation holes 35, 36, 37, 38 in upper and lower positions, and the elution heating grooves 31, 32, 33, 34 protrude to the upper surface of the first heating body 20 through the clearance holes 25, 26, 27, 28.

[0062] In this embodiment, the protruding height of the elution heating grooves 31, 32, 33, and 34 on the second heating body 30 is greater than the protruding height of the cleavage heating grooves 21, 22, 23, and 24 on the first heating body 20. When the first heating body 20 and the second heating body 30 are docked, the surface height of the cleavage heating grooves 21, 22, 23, and 24 is consistent with the surface height of the elution heating grooves 31, 32, 33, and 34, and the test kit can be placed at the same height in the test kit fixing position.

[0063] The implementation principle of the embodiment of this application is:

[0064] When assembling the heating device, first align the first heating body 20 and the second heating body 30 up and down, align the clearance holes 25, 26, 27, 28 on the first heating body 20 with the elution heating grooves 31, 32, 33, 34 on the second heating body 30, and then lower them until the elution heating grooves 31, 32, 33, 34 are exposed and flush with the cracking heating grooves 21, 22, 23, 24, and then fix the first heating body 20 and the second heating body 30 with bolts.

[0065] During the nucleic acid extraction process, a 32-person nucleic acid extraction kit for the one-step elution method is placed on the kit fixing position, and the lysis step kit is heated separately by the lysis heating tanks 21, 22, 23, and 24, and the elution step kit is heated separately by the elution heating tanks 31, 32, 33, and 34, so that the heating device of the nucleic acid extractor can match the heating requirements of the lysis and elution steps of the 32-person nucleic acid extraction kit for the one-step elution method. The lysis and elution are heated in steps, which improves the heating efficiency, reduces heat radiation and excessive volatilization of the reagent, ensures the stability of the reagent concentration, and improves the efficiency and quality of nucleic acid extraction.

[0066] The present application also proposes a nucleic acid extractor, including the above-mentioned heating device. The heating device is installed in the shell of the nucleic acid extractor. The nucleic acid extractor can heat 96 servings, that is, 32 servings of nucleic acid extraction kits of three one-step elution methods at the same time. While the size of the entire machine is not much different from that of a 32-serving nucleic acid extraction kit of a two-step elution method, the throughput is 96 servings, which greatly improves the efficiency and speed of nucleic acid extraction.

[0067] The examples of this specific embodiment are all preferred embodiments of this application and are not intended to limit the scope of protection of this application. Identical components are represented by the same reference numerals. Therefore, any equivalent changes made based on the structure, shape, and principle of this application should be included in the scope of protection of this application.

Claims

1. A heating device, characterized in that: The invention comprises a heating body (10) having a reagent box fixing position on its surface, the heating body (10) comprising a first heating body (20) and a second heating body (30) which are relatively detachably arranged up and down, the upper surfaces of the first heating body (20) and the second heating body (30) are both provided with a plurality of rows of heating units, and the heating units on the second heating body (30) pass upward through the first heating body (20) to the upper surface of the first heating body (20) to supplement the heating positions on the upper surface of the first heating body (20); The first heating body (20) has three reagent box fixing positions on its upper surface, and the back of the first heating body (20) corresponding to the three reagent box fixing positions has three first electric heating films (51, 52, 53), and the back of the second heating body (30) corresponding to the three reagent box fixing positions has three second electric heating films (71, 72, 73); Each reagent kit fixing position on the first heating body (20) is provided with a first row of lysis heating tanks (21), a fourth row of lysis heating tanks (22), a seventh row of lysis heating tanks (23) and a tenth row of lysis heating tanks (24). Below each reagent kit fixing position on the second heating body (30), there are respectively a third row of elution heating slots (31), a sixth row of elution heating slots (32), a ninth row of elution heating slots (33), and a twelfth row of elution heating slots (34); The first heating body (20) is provided with clearance holes (25, 26, 27, 28) at the 3rd, 6th, 9th and 12th columns of each reagent box fixing position, respectively. The clearance holes (25, 26, 27, 28) respectively accommodate the 3rd column elution heating tank (31), the 6th column elution heating tank (32), the 9th column elution heating tank (33) and the 12th column elution heating tank (34) on the second heating body (30); The first heating body (20) is provided with a first heat preservation device (41, 42, 43) on the back side corresponding to each reagent box fixing position, and the first heat preservation device (41, 42, 43) is connected to the first electric heating film (51, 52, 53) in a one-to-one correspondence to control the power on or off of the first electric heating film (51, 52, 53); The second heating body (30) is provided with a second heat preservation device (61, 62, 63) on the back side corresponding to each reagent box fixing position. The second heat preservation devices (61, 62, 63) are connected to the second electric heating films (71, 72, 73) in a one-to-one correspondence to control the power on or off of the second electric heating films (71, 72, 73).

2. A heating device according to claim 1, characterized in that: The protruding height of the elution heating groove on the second heating body (30) is greater than the protruding height of the cracking heating groove on the first heating body (20). When the first heating body (20) and the second heating body (30) are connected, the surface height of the cracking heating groove is consistent with the surface height of the elution heating groove.

3. A heating device according to claim 1, characterized in that: Heat dissipation holes (35, 36, 37, 38) are respectively provided at the first, fourth, seventh and tenth columns of each reagent box fixing position on the second heating body (30).

4. A heating device according to claim 1, characterized in that: The thermal insulation device includes a temperature sensor and a controller, and the controller sets the upper temperature limit; When the temperature sensor senses that the temperature of the first heating body (20) and / or the second heating body (30) exceeds the upper temperature limit, the controller controls the electric heating film to be disconnected; When the temperature sensor senses that the temperature of the first heating body (20) and / or the second heating body (30) has dropped below the upper temperature limit, the controller controls the electric heating film to be energized.

5. A heating device according to claim 1, characterized in that: First temperature measuring probes (81, 82, 83) are respectively provided on the three first electric heating films (51, 52, 53); Second temperature measuring probes (91, 92, 93) are respectively provided on the three second electric heating films (71, 72, 73).

6. A nucleic acid extraction instrument, characterized in that: The heating device comprises the heating device according to any one of claims 1 to 5.

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