Centrifugal cup, cell centrifugation method and centrifugal device

Abstract

The present invention relates to a centrifugal cup, a cell centrifugation method, and a centrifugal device. The centrifugal cup includes a cup bowl, a cup cover, a first region partition plate, a second region partition plate, and a third region partition plate. A preset gap exists between the first region partition plate and the inner wall of the cup bowl at one end of the side wall facing the cup bowl. Drainage ports are formed between the outer end of the second region partition plate and the inner wall of the cup bowl, and between the outer end of the third region partition plate and the inner wall of the cup bowl. The cup cover and / or the cup bowl are provided with an inlet channel and an outlet channel. The inlet channel opening of the inlet channel is located within the preset gap, and the distance between the inlet channel opening and the inner wall of the cup bowl is within a first preset range. The outlet channel opening of the outlet channel is located within a second preset range from the inner wall of the cup bowl and is located between the second region partition plate and the third region partition plate. Based on continuous flow concentration, the above-mentioned centrifugal cup achieves low escape and rapid concentration under high speed and high flow conditions, thereby simultaneously improving both yield and activity.

Description

Technical Field

[0001] The present invention relates to the technical field of cell processing, and in particular to a centrifugal cup, a cell centrifugation method and a centrifugal device. Background Art

[0002] In the field of cell processing, it is necessary to concentrate the cell fluid, that is, to concentrate the low-concentration cell fluid into a high-concentration cell fluid, so as to facilitate subsequent cell processing, such as storage and transportation. For this purpose, centrifugal sedimentation is usually used to concentrate cells: the cell fluid is added to the centrifuge cup, and the cells are pushed to the edge of the centrifuge cup by centrifugal force at a high speed. Finally, the supernatant near the center of rotation in the centrifuge cup is extracted to complete the cell fluid concentration. In the process of concentrating cells by centrifugal sedimentation, the yield and viability are the two characteristic indicators to pay attention to. The yield is a measure of the proportion of cells escaping from the centrifuge cup outlet during concentration, that is, the yield = the number of live cells after treatment / the number of live cells before treatment. The viability is a measure of the proportion of cells that survive the fluid shear caused by centrifugation, that is, the viability = the number of live cells / the total number of cells.

[0003] Currently, most centrifuge cups will cause severe shear damage to cells at speeds above 3000 rpm, resulting in a low yield. At speeds below 2500 rpm, high flow rates cannot be used, which in turn reduces the speed and cell sedimentation rate, resulting in a low viability. Therefore, in existing continuous flow concentration technology, centrifuge cups at high flow rates and high speeds result in low yield and viability, resulting in unsatisfactory concentration effects. Summary of the Invention

[0004] The present invention provides a centrifugal cup, a cell centrifugation method and a centrifugal device. The centrifugal cup optimizes the flow field and changes the cell trajectory on the basis of continuous flow concentration, thereby achieving less escape and rapid concentration under high rotation speed and high flow, thereby simultaneously improving the yield and viability.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The present application provides a centrifugal cup, which includes a cup bowl, a cup cover, a first area partition plate, a second area partition plate, and a third area partition plate;

[0007] The first region partition plate extends from the center of the cup bowl toward the side wall of the cup bowl, and a preset gap is formed between the end facing the side wall of the cup bowl and the inner wall of the cup bowl; a drain port is formed between the outer end of the second region partition plate and the inner wall of the cup bowl, and between the outer end of the third region partition plate and the inner wall of the cup bowl;

[0008] The cup cover and / or the cup bowl are provided with a liquid inlet channel and a liquid outlet channel; the liquid inlet channel opening of the liquid inlet channel is located within the preset gap, and the distance between the liquid inlet channel opening and the inner wall of the cup bowl is within a first preset range; the distance between the liquid outlet channel opening and the inner wall of the cup bowl is within a second preset range, and is located between the second area partition plate and the third area partition plate.

[0009] Furthermore, there is a preset gap between one end of the side wall facing the cup bowl and the inner wall of the cup bowl, comprising:

[0010] The first region partition plate is provided with an end plate at one end of the side wall facing the cup bowl, and a gap is formed between the end plate and the inner wall of the cup bowl.

[0011] Furthermore, the end plate is arc-shaped, and its arc length is greater than or equal to 5 mm.

[0012] Furthermore, the first preset range is 0.1 mm to 20 mm.

[0013] Furthermore, the second preset range is greater than or equal to 4 mm.

[0014] Furthermore, the first region partition plate, the second region partition plate and the third region partition plate are sequentially spaced apart along the rotation direction of the cup bowl;

[0015] The first area partition plate, the second area partition plate and the third area partition plate all extend radially along the cup bowl and are distributed in a "Y" shape. The first area partition plate is fixedly connected to the cup cover or the cup bowl provided with the liquid inlet channel, and the second area partition plate and the third area partition plate are fixedly connected to the cup cover or the cup bowl.

[0016] Furthermore, the drain port formed between the outer end of the second region partition plate and the inner wall of the cup bowl, and the drain port formed between the outer end of the third region partition plate and the inner wall of the cup bowl are both smaller than the distance between the liquid inlet port and the inner wall of the cup bowl.

[0017] Furthermore, the second region partition plate and the third region partition plate are distributed on both sides of the liquid outlet.

[0018] In addition, the present application provides a cell centrifugation method, wherein the cell centrifugation method is performed by performing a cell centrifugation operation based on any one of the above-mentioned centrifugation cups, and the cell centrifugation method comprises:

[0019] The liquid to be treated flows into the liquid inlet through the liquid inlet flow channel;

[0020] The centrifugal cup is driven to rotate by a rotating device, so that the liquid to be treated flows in an annular direction from the periphery of the preset gap to the discharge port for centrifugation; the centrifugal cup is connected to the rotating device;

[0021] During the centrifugation process, the effluent is extracted through the effluent flow channel; the effluent is the liquid adjacent to the effluent flow channel after the liquid to be treated is centrifuged.

[0022] The present application also provides a centrifugal device, which includes any one of the above-mentioned centrifugal cups.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] 1. The centrifuge cup of the present invention is provided with a first region partition plate, a second region partition plate and a third region partition plate, and the flow of the cell fluid is controlled by the three region partition plates. A preset gap is formed between one end of the side wall of the first region partition plate facing the cup bowl and the inner wall of the cup bowl, and the liquid inlet flow channel for transporting the cell fluid into the cup bowl is arranged in the preset gap, so that the position of the liquid inlet flow channel for transporting the cell fluid into the cup bowl is far away from the rotation center, providing a larger centrifugal force for cell sedimentation. At the same time, the cell fluid enters the preset gap from the liquid inlet flow channel, and the liquid flow direction is changed to a tangential direction. Under this flow mode, the effect of fluid disturbance is less than the effect of centrifugal sedimentation, and the cells gradually adhere to the wall; as the liquid carries the cells and continues to flow along the circumferential wall, the fluid disturbance is further reduced, and the cells are fully settled to the wall; the structural design of the preset gap and the liquid inlet flow channel can control the liquid inlet speed and acceleration, thereby controlling the cell trajectory, achieving rapid cell sedimentation, and improving the centrifugal yield and viability;

[0025] 2. The centrifuge cup of the present invention is provided with three regional partitions arranged in a "Y" shape. An inlet area for cell fluid is formed between the arc-shaped end plate and the inner wall of the cup bowl. The inlet area is used to change the flow direction of the incoming liquid from radial to tangential. A development area is formed between the first and second regional partitions. The development area is used to allow the cell fluid to continue to flow along the circumferential wall along with the liquid carrying the cells, further reducing fluid disturbance and allowing the cells to fully settle to the wall. An outlet area is formed between the second and third regional partitions. The outlet area is used to form a huge vortex to complete the cell-supernatant separation, thereby improving the centrifugal yield and viability.

[0026] 3. The centrifuge cup of the present invention can form drainage holes between the second region partition plate and the inner wall of the cup bowl, and between the third region partition plate and the inner wall of the cup bowl, by providing the second region partition plate and the third region partition plate. The second region partition plate and the third region partition plate can provide a flow field similar to the preset gap. The gap formed by the drainage hole guides the cells to move along the circumferential wall, forcing the cells to settle close to the circumferential wall, further avoiding cell disturbance and allowing the cells to remain attached to the wall and continue to settle. At the same time, the second region partition plate and the third region partition plate are close to the liquid outlet. When the cells adhere to the wall, the liquid flows out from the liquid outlet, avoiding the cells from being lifted near the liquid outlet, thereby reducing cell loss, thereby improving the centrifugal yield and viability.

[0027] 4. The present invention sets the liquid outlet between the second and third area partition plates, so that the liquid forms a huge vortex between the second and third area partition plates, thereby completing the cell-supernatant separation gently and clearly, thereby improving the centrifugal yield and viability.

[0028] Therefore, the centrifugal cup with the above structure can optimize cell movement in terms of both liquid speed and acceleration, reduce cell escape under low speed and high flow conditions, achieve rapid concentration of cell fluid, and thus simultaneously improve yield and viability. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the explosion structure of the centrifugal cup of the present invention;

[0030] Figure 2 is a cross-sectional view of the centrifugal cup of the present invention;

[0031] Figure 3 This is a top view of the centrifugal cup of the present invention with the cup cover removed;

[0032] Figure 4 Flow chart of the cell centrifugation method of the present invention.

[0033] Among them, 1-cup bowl, 2-cup cover, 3-first area partition, 4-second area partition, 5-third area partition, 6-end plate, 7-preset gap, 8-first drain port, 9-second drain port, 10-liquid inlet channel, 11-liquid outlet channel, 12-liquid inlet, 13-liquid outlet, 14-liquid inlet channel outlet, 15-liquid outlet channel outlet. DETAILED DESCRIPTION

[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0035] Example 1

[0036] This embodiment provides a centrifugal cup, such as Figure 1 、 Figure 2 and Figure 3 As shown in the structure, the centrifugal cup includes a cup bowl 1, a cup cover 2, a first area partition 3, a second area partition 4 and a third area partition 5; a circular cavity for accommodating cell fluid is formed in the cup bowl 1; the cup cover 2 is sealed on the top of the cup bowl 1; the radius and height of the cup bowl 1 can be set according to needs, the radius of the cup bowl 1 can be 30mm~80mm, the height of the cup bowl 1 can also be 30mm~80mm, the radius of the cup bowl 1 is preferably 30mm, and the height of the cup bowl 1 is preferably 35mm.

[0037] The extension direction of the first area partition plate 3 is from the center of the cup bowl 1 toward the side wall of the cup bowl 1, and there is a preset gap between the inner wall of the cup bowl 1 and the end facing the side wall of the cup bowl 1; drainage ports are formed between the outer end of the second area partition plate 4 (that is, the end close to the inner wall of the cup bowl 1) and the inner wall of the cup bowl 1, and between the outer end of the third area partition plate 5 (that is, the end close to the inner wall of the cup bowl 1) and the inner wall of the cup bowl 1.

[0038] The cup cover 2 and / or the cup bowl 1 is provided with a liquid inlet channel 10 and a liquid outlet channel 11; the liquid inlet channel opening 14 of the liquid inlet channel 10 is located within the preset gap 7, and the distance between the liquid inlet channel opening 14 and the inner wall of the cup bowl 1 is within a first preset range; the distance between the liquid outlet channel opening 15 of the liquid outlet channel 11 and the inner wall of the cup bowl 1 is within a second preset range, and is located between the second area partition plate 4 and the third area partition plate 5, wherein the first preset range and the second preset range are set according to demand, for example: the first preset range is 0.01 to 30 mm, the second preset range is 4 mm to 20 mm, and so on.

[0039] In one embodiment, the first preset range is 0.1 mm to 20 mm.

[0040] In one embodiment, the second preset range is greater than or equal to 4 mm.

[0041] In one embodiment, when the cell fluid needs to be concentrated by the centrifugal cup, the bowl 1 rotates in a clockwise direction. Figure 3As shown, along the rotation direction of the cup bowl 1, the first area partition plate 3, the second area partition plate 4 and the third area partition plate 5 are sequentially spaced apart; the first area partition plate 3, the second area partition plate 4 and the third area partition plate 5 can also be sequentially spaced apart along the opposite direction of the rotation of the cup bowl 1. The first area partition plate 3, the second area partition plate 4 and the third area partition plate 5 all extend along the radial direction of the cup bowl 1 and are distributed in a "Y" shape. The first area partition plate 3 is fixedly connected to the cup cover 2 or the cup bowl 1 provided with the liquid inlet channel 10. , the second area partition plate 4 and the third area partition plate 5 are fixedly connected to the cup cover 2 or the cup bowl 1, and there are gaps between the second area partition plate 4 and the first area partition plate 3, between the second area partition plate 4 and the third area partition plate 5, and between the third area partition plate 5 and the first area partition plate 3; the angle between the second area partition plate 4 and the third area partition plate 5 is 10° to 120°, such as: 10°, 20°, 30°, 40°, 50°, 60°, 80°, 90°, 100°, 120°; Figure 3 As shown in the structure, preferably, the angle between the second region partition plate 4 and the third region partition plate 5 is 60°.

[0042] The first-region partition plate 3 extends from the center of the cup bowl 1 toward the side wall of the cup bowl 1, and there is a preset gap 7 between the end of the side wall facing the cup bowl 1 and the inner wall of the cup bowl 1. In one embodiment, an end plate 6 is provided at one end of the side wall facing the cup bowl 1, and a gap is formed between the end plate 6 and the inner wall of the cup bowl 1. The end plate 6 can help reduce damage to cells when liquid is introduced into the liquid inlet channel 14, and the gap forms a preset gap 7. In one embodiment, an end plate 6 is provided at one end of the side wall facing the cup bowl 1. In one embodiment, the curvature of the end plate 6 can be the same as the curvature of the inner wall of the cup bowl 1, thereby forming an arc-shaped gap between the end plate 6 and the inner wall of the cup bowl 1; one end of the first-region partition plate 3 is provided near the center of the cup bowl 1 and is aligned with the rotation axis of the cup bowl 1. The centers of the two sides coincide, and the other end is close to the inner wall of the cup bowl 1 and is fixedly connected to the end plate 6; the arc length of the end plate 6 is greater than or equal to 5 mm; a first drain port 8 is formed between the outer end portion of the second-region partition plate 4 and the inner wall of the cup bowl 1, and a second drain port 9 is formed between the outer end portion of the third-region partition plate 5 and the inner wall of the cup bowl 1; the distances between the end plate 6, the second-region partition plate 4 and the third-region partition plate 5 and the side wall of the cup bowl 1 are all 0.1 mm to 10 mm; the distance between the second-region partition plate 4 and the inner wall of the cup bowl 1 can be 3 mm; the distance between the third-region partition plate 5 and the inner wall of the cup bowl 1 can be 3 mm, and the distance between the liquid inlet port 14 and the side wall of the cup bowl 1 is less than the distance between the end plate 6 and the side wall of the cup bowl 1.

[0043] The shape of the end plate 6 can be set according to needs, for example, the shape of the end plate 6 is arc-shaped, T-shaped, triangular, etc.

[0044] In one embodiment, the first drain port 8 formed between the outer end of the second region partition plate 4 and the inner wall of the cup bowl 1, and the second drain port 9 formed between the outer end of the third region partition plate 5 and the inner wall of the cup bowl 1 are both smaller than the distance between the liquid inlet channel 14 and the inner wall of the cup bowl 1. The cup cover 2 and / or the cup bowl 1 are provided with a liquid inlet channel 10 and a liquid outlet channel 11, that is, the liquid inlet channel 10 can be provided on the cup cover 2 or the cup bowl 1, or can be provided on both the cup cover 2 and the cup bowl 1, and the same applies to the liquid outlet channel 11; Figure 2 and Figure 3 As shown in the structure, the liquid inlet channel 10 first extends along the center of the cup cover 2 and the cup bowl 1 to the inner bottom surface of the cup bowl 1, and then continues to extend along the bottom edge of the first area partition plate 3 to the preset gap 7, connecting the liquid inlet 12 and the liquid inlet channel opening 14; the liquid outlet channel 11 extends along the radial direction of the rotation center and the bottom surface of the cup cover 2, connecting the liquid outlet 13 and the liquid outlet channel opening 15; the liquid inlet 12 of the liquid inlet channel 10 and the liquid outlet 13 of the liquid outlet channel 11 can both be located at the top of the cup cover 2; the liquid inlet channel opening 14 of the liquid inlet channel 10 is located in the preset gap 7, and the liquid inlet channel opening 14 is in contact with the cup bowl 1 The distance between the inner wall of the cup is 5mm; the outlet flow channel 11 is set at the bottom of the cup cover 2, and the distance between it and the inner wall of the cup bowl 1 is 20mm to 30mm. The distance between the outlet flow channel 15 and the inner wall of the cup bowl 1 is preferably 25mm; the outlet flow channel 15 is located between the second area partition 4 and the third area partition 5; the second area partition 4 and the third area partition 5 are symmetrically distributed on both sides of the outlet flow channel 15, and the second area partition 4 and the third area partition 5 can also be approximately symmetrically distributed on both sides of the outlet flow channel 15. The area of ​​the liquid inlet flow channel 14 and the outlet flow channel 15 is less than 9mm 2 The area of ​​the liquid inlet flow channel 14 and the liquid outlet flow channel 15 is preferably 4mm 2 , and the liquid inlet flow channel opening 14 and the liquid outlet flow channel opening 15 are both square openings.

[0045] The centrifugal cup is provided with a first area partition plate 3, a second area partition plate 4 and a third area partition plate 5, and the flow control of the cell fluid is achieved by the three area partition plates. An arc-shaped end plate 6 is provided at one end of the side wall of the first area partition plate 3 facing the cup bowl 1, and an arc-shaped gap is formed between the end plate 6 and the inner wall of the cup bowl 1, and the inlet flow channel 14 for transporting cell fluid into the cup bowl 1 is provided in the arc-shaped gap, forming an inlet area of ​​the cell fluid in the arc-shaped gap, so that the position of the inlet flow channel 14 for transporting cell fluid into the cup bowl 1 is far away from the rotation center, providing a larger centrifugal force for cell sedimentation, and at the same time, the cell fluid enters the arc-shaped gap from the inlet flow channel 14, and is constrained by the gap wall, and the liquid flow direction is The direction is changed to the tangential direction, the influence of fluid disturbance is smaller than that of centrifugal sedimentation, and the cells gradually adhere to the wall; as the liquid carries the cells to continue to flow along the circumferential wall, the fluid disturbance is further reduced, and the cells are fully settled to the wall; the structural design of the arc-shaped gap and the liquid inlet flow channel 14 can control the liquid inlet speed and acceleration, thereby controlling the cell trajectory and achieving rapid cell sedimentation; by setting the end plate 6 at the end of the partition plate 3 in the first area, the cell fluid flowing into the cup 1 can enter the arc-shaped gap from the liquid inlet flow channel 14, and the cell fluid forms a circumferential liquid flow. At this time, the cell speed is along the circumferential direction, which is convenient for controlling the initial movement characteristics and initial trajectory of the cells. Controlling the initial trajectory of the cells is the key to continuous cell sedimentation.

[0046] The centrifugal cup is provided with a second region partition plate 4 and a third region partition plate 5, which can form a first drain port 8 between the second region partition plate 4 and the inner wall of the cup bowl 1, and a second drain port 9 between the third region partition plate 5 and the inner wall of the cup bowl 1. The second region partition plate 4 and the third region partition plate 5 can provide a flow field similar to an arc-shaped gap between the cup bowl 1, and the gap formed by the drain port guides the cells to move along the circumferential wall, forcing the cells to settle close to the circumferential wall, further avoiding cell disturbance, and allowing the cells to maintain an adherent state and continue to settle; the liquid outlet 15 When the distance between the centrifugal cup and the inner wall of the bowl 1 is within the second preset range, preferably, the second preset range is 20 mm to 30 mm, so that when the centrifugal cup rotates, the liquid is centrifuged to form an annular liquid surface, and when the liquid is higher than the outlet flow channel 15, it is quickly drawn away to stabilize the liquid surface, ensure that the fluid trajectory is consistent and easy to control, and further ensure the liquid flow control effect of the first drain port 8; at the same time, the second area partition plate 4 and the third area partition plate 5 are close to the outlet flow channel 15, and the liquid flows out from the outlet flow channel 15 when the cells adhere to the wall, avoiding the cells from being lifted near the outlet flow channel 15, thereby reducing cell loss.

[0047] The centrifugal cup is provided with a liquid outlet 15 at the bottom of the cup cover 2, so that the liquid outlet 15 is located at the top of the cup bowl 1. The liquid reaching the liquid outlet 15 can flow out of the centrifugal cup through the provided liquid outlet 15, thereby locking the liquid level position in the centrifugal cup and keeping the liquid level in the centrifugal cup stable.

[0048] The centrifuge cup is provided with three regional partitions distributed in a "Y" shape on the cup cover 2 or the cup bowl 1, and an inlet area for the cell fluid is formed between the arc-shaped end plate 6 and the inner wall of the cup bowl 1, and the inlet area is used to change the flow direction of the incoming liquid from radial to tangential; a development area is formed between the first regional partition 3 and the second regional partition 4, and the development area is used to allow the cell fluid to continue to flow along the circumferential wall surface along with the liquid carrying the cells, and the fluid disturbance is further reduced, and the cells are fully settled to the wall. Through the development area, the flow law at the arc-shaped gap can be followed, and the effect of cell sedimentation can be further consolidated; by setting the outlet flow channel 15 between the second regional partition 4 and the third regional partition 5, an outlet area is formed between the second regional partition 4 and the third regional partition 5, and the outlet area is used to allow the liquid to form a huge vortex between the second regional partition 4 and the third regional partition 5, which can complete the separation of cells and supernatant, thereby improving the centrifugal yield and activity.

[0049] Therefore, the centrifugal cup with the above structure can optimize cell movement in terms of both liquid speed and acceleration, reduce cell escape under high speed and high flow conditions, achieve rapid concentration of cell fluid, and thus simultaneously improve yield and viability.

[0050] Furthermore, radial gaps are formed between the second region partition plate 4 and the first region partition plate 3 , and between the third region partition plate 5 and the first region partition plate 3 .

[0051] Verified by T cells, after multiple concentration experiments, the yield and viability of human T cells concentrated using the above centrifugal cup are shown in Table 1:

[0052]

[0053]

[0054] Table 1 Results of human T cell concentration test

[0055] The above-mentioned centrifugal cup can realize continuous flow concentration of cell fluid, forming a continuous flow between the liquid inlet and the liquid outlet. The flow rate of the cell fluid can reach 100ml / min~250ml / min per minute, and during the concentration process, the rotation speed of the centrifugal cup can be 2000rpm~2500rpm.

[0056] When the centrifugal cup is provided with a liquid outlet 15 at the bottom of the cup cover 2, the liquid outlet 15 is located at the top of the cup bowl 1. The liquid reaching the liquid outlet 15 can flow out of the centrifugal cup through the provided liquid outlet 15, thereby locking the liquid level position in the centrifugal cup and keeping the liquid level in the centrifugal cup stable.

[0057] Example 2

[0058] This embodiment provides a cell centrifugation method, such as Figure 4 As shown, the cell centrifugation method uses the centrifuge cup of the above embodiment to perform cell centrifugation operation, and the cell centrifugation method includes the following steps:

[0059] Step S10 , the liquid to be treated flows into the liquid inlet port 14 .

[0060] Step S20: The centrifugal cup is driven to rotate by a rotating device, so that the liquid to be treated flows in an annular direction from the periphery of the preset gap 7 to the discharge port for centrifugation; the centrifugal cup is connected to the rotating device.

[0061] Step S30 , extracting effluent through the effluent outlet 15 during the centrifugation process; the effluent is the liquid adjacent to the effluent outlet 15 after the liquid to be treated is centrifuged.

[0062] The liquid to be treated is a solution that needs to be centrifuged for cells. The liquid to be treated flows in from the liquid inlet flow channel 14, and an inlet area for the cell liquid is formed between the arc-shaped end plate 6 and the inner wall of the cup bowl 1. The inlet area is used to change the flow direction of the incoming liquid from radial to tangential; a development area is formed between the first area partition plate 3 and the second area partition plate 4. The development area is used to allow the cell liquid to continue to flow along the circumferential wall surface along with the liquid carrying the cells, and the fluid disturbance is further reduced, and the cells are fully settled to the wall. During the rotation of the centrifuge cup, the flow law at the preset gap 7 can be followed through the development area to continue to consolidate the effect of cell sedimentation; by setting the liquid outlet flow channel 15 between the second area partition plate 4 and the third area partition plate 5, in the second area An outlet area is formed between the regional partition plate 4 and the third regional partition plate 5, and the outlet area is used to form a huge vortex between the second regional partition plate 4 and the third regional partition plate 5. Therefore, the present invention realizes the flow of the liquid to be treated through the liquid inlet flow channel 14; the centrifugal cup is driven to rotate by the rotating device, so that the liquid to be treated flows in a circumferential direction from the periphery of the preset gap 7 to the discharge port for centrifugation; the centrifugal cup is connected to the rotating device; during the centrifugation process, the effluent is extracted through the liquid outlet flow channel 15. In this way, the separation of cells and supernatant can be completed gently and clearly, thereby extracting the effluent. The effluent is the liquid adjacent to the liquid outlet flow channel 15 after the liquid to be treated is centrifuged, thereby improving the yield and activity of the effluent obtained by centrifugation.

[0063] Example 3

[0064] This embodiment provides a centrifugal device, which includes the centrifugal cup in the above embodiment. The centrifugal cup is used to perform cell centrifugation operation, which is an operation process of concentrating cells by using centrifugal sedimentation.

[0065] Since the centrifugal device adopts the centrifugal cup, it can realize continuous flow concentration of cell fluid and improve the yield and activity of cell fluid during the centrifugation process.

[0066] Obviously, those skilled in the art may make various changes and modifications to the embodiments of the present invention without departing from the spirit and scope of the present invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is intended to include such modifications and variations.

Claims

1. A centrifugal cup, characterized in that: The invention comprises a cup bowl, a cup cover, a first area partition plate, a second area partition plate, and a third area partition plate; The first region partition plate extends from the center of the cup bowl toward the side wall of the cup bowl, and a preset gap is formed between the end facing the side wall of the cup bowl and the inner wall of the cup bowl; a drain port is formed between the outer end of the second region partition plate and the inner wall of the cup bowl, and between the outer end of the third region partition plate and the inner wall of the cup bowl; The cup cover and / or the cup bowl are provided with a liquid inlet channel and a liquid outlet channel; the liquid inlet channel opening of the liquid inlet channel is located within the preset gap, and the distance between the liquid inlet channel opening and the inner wall of the cup bowl is within a first preset range; the liquid outlet channel opening of the liquid outlet channel is within a second preset range between the inner wall of the cup bowl, and is located between the second region partition plate and the third region partition plate; The first region partition plate, the second region partition plate and the third region partition plate are sequentially spaced apart along the rotation direction of the cup bowl; The first region partition plate, the second region partition plate, and the third region partition plate all extend radially along the cup bowl and are distributed in a "Y" shape. The first region partition plate is fixedly connected to the cup cover or the cup bowl provided with the liquid inlet flow channel, and the second region partition plate and the third region partition plate are fixedly connected to the cup cover or the cup bowl. The drain opening formed between the outer end of the second region partition plate and the inner wall of the cup bowl, and the drain opening formed between the outer end of the third region partition plate and the inner wall of the cup bowl are both smaller than the distance between the liquid inlet opening and the inner wall of the cup bowl; The second region partition plate and the third region partition plate are distributed on both sides of the liquid outlet.

2. The centrifuge cup according to claim 1, wherein: The method of providing a preset gap between one end of the side wall facing the cup bowl and the inner wall of the cup bowl comprises: An end plate is provided at one end of the side wall facing the cup bowl, and a gap is formed between the end plate and the inner wall of the cup bowl.

3. The centrifuge cup according to claim 2, characterized in that The end plate is arc-shaped, and the arc length is greater than or equal to 5 mm.

4. The centrifuge cup according to claim 1, wherein: The first preset range is 0.1 mm to 20 mm.

5. The centrifuge cup according to claim 1, wherein: The second preset range is greater than or equal to 4 mm.

6. A cell centrifugation method, characterized in that The cell centrifugation method is performed by performing a cell centrifugation operation based on the centrifuge cup according to any one of claims 1 to 5, and the cell centrifugation method comprises: The liquid to be treated flows into the liquid inlet through the liquid inlet flow channel; The centrifugal cup is driven to rotate by a rotating device, so that the liquid to be treated flows in an annular direction from the periphery of the preset gap to the discharge port for centrifugation; the centrifugal cup is connected to the rotating device; During the centrifugation process, the effluent is extracted through the effluent flow channel; the effluent is the liquid adjacent to the effluent flow channel after the liquid to be treated is centrifuged.

7. A centrifugal device, characterized in that: The centrifuge cup comprises the centrifuge cup according to any one of claims 1 to 5.

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