A plant tissue sampling device

Abstract

The application discloses a plant tissue sampling device, which comprises a base, a centrifugal tube rack, a fixing frame and a punching device. The base is provided with a U-shaped placing groove, and a sliding groove matched with the centrifugal tube rack is arranged on the upper portion of the U-shaped placing groove. The fixing frame and the punching device are arranged above the U-shaped placing groove. Before sampling operation, the centrifugal tube can be placed in the centrifugal tube placing groove on the centrifugal tube rack, and the plant tissue such as leaf blade is placed above the centrifugal tube. The punching device is pressed to punch and sample the plant tissue such as leaf blade. The sampled plant tissue directly falls into the centrifugal tube, and manual transfer is not needed, so that the operation is convenient and fast. A plurality of groups of sampling operations can be simultaneously conducted by moving the centrifugal tube rack, and the efficiency is further improved. The device can be matched with centrifugal tube racks of different specifications, and can be matched with punching devices of different specifications, so that the sampling requirements of different tests and different centrifugal tubes can be met. The device can be matched with punching devices of different numbers, and the sampling efficiency is further improved.

Description

Technical Field

[0001] This invention relates to the field of plant tissue sampling devices, and more particularly to a plant tissue sampling device. Background Technology

[0002] In plant genome extraction experiments, tissue samples must first be collected and placed into centrifuge tubes before subsequent experimental operations such as separation and extraction can be performed. There are two sampling methods: 1. Leaf samples are directly collected from the field or outdoors and placed into centrifuge tubes; 2. Leaves are processed (e.g., freeze-dried or air-dried) and then aliquoted into centrifuge tubes in the laboratory. Generally, leaf samples placed into centrifuge tubes need to be processed to a size of 2mm-5mm. During laboratory sampling and aliquoting, a hole punch is typically used. Traditional hole punches lack a device to hold the centrifuge tubes in place; after punching the hole and taking the sample, the sample is then placed into the centrifuge tube, making the operation cumbersome and prone to confusing different experimental groups.

[0003] On the other hand, traditional punchers produce tissue samples in only one size, which cannot meet the requirements of different types of genomic experiments, nor can they be adapted to centrifuge tubes with different pore sizes. Summary of the Invention

[0004] To address the above shortcomings, this invention provides a plant tissue sampling device that solves the problems of cumbersome and inefficient sampling operations in existing plant genome extraction experiments.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A plant tissue sampling device, comprising:

[0007] A base, wherein a U-shaped placement groove is provided on the base, and a sliding groove is provided on the upper part of the U-shaped placement groove, and the sliding groove is horizontally arranged;

[0008] Centrifuge tube rack, with both sides of the centrifuge tube rack mounted on the slide groove and able to slide horizontally relative to the slide groove, and centrifuge tube placement holes are provided at intervals on the centrifuge tube rack;

[0009] A fixing frame is provided above the base and the centrifuge tube rack, and the fixing frame is provided with fixing holes;

[0010] A drilling device is mounted on a fixed frame and passes through a fixed hole. The drilling device includes a limiting cylinder, a connecting tube, a limiting block, a drilling knife, a pressing rod, and a pressing block. The connecting tube is hollow and passes through the fixed hole. One end of the connecting tube is connected to the limiting cylinder, and the other end is connected to the limiting block. The limiting block has a through hole located above the fixed hole. The limiting cylinder has an opening at the bottom and a through hole at the top. The pressing rod is located inside the connecting tube and passes through the through hole in the limiting block and the through hole in the limiting cylinder. One end of the pressing rod is connected to the drilling knife, and the other end is connected to the pressing block. The drilling knife is located inside the limiting cylinder. A spring is also sleeved on the outer periphery of the pressing rod. The spring is located between the pressing block and the limiting block, or between the drilling knife and the limiting cylinder.

[0011] Preferably, the punching device further includes a pressing block, which is connected to the inner side of the top of the punching knife by a spring, and the end of the pressing block is flush with the blade of the punching knife.

[0012] Preferably, the punch is a hollow cylinder with one end open, and the cutting edge of the punch is serrated.

[0013] Preferably, the height of the punching tool is less than the height of the limiting cylinder.

[0014] Preferably, the end of the pressing rod is provided with an internally threaded mounting hole, and the top of the punching tool is fixed with an externally threaded connecting part, and the punching tool is detachably connected to the pressing rod through the connecting part.

[0015] Preferably, the fixing frame is provided with a fixing groove, the fixing hole is provided in the fixing groove, the shape and size of the fixing groove are matched with the limiting block of the punching device, the limiting block is provided at the fixing groove, and an elastic element is provided between the bottom of the limiting block and the bottom of the fixing groove, the elastic force of the elastic element is less than the elastic force of the spring on the outer periphery of the pressing rod.

[0016] Preferably, the fixing frame is equipped with multiple punching devices.

[0017] Preferably, multiple U-shaped placement slots and centrifuge tube racks are also provided.

[0018] Preferably, an elastic gasket is provided inside the centrifuge tube placement hole. The elastic gasket facilitates the placement of the centrifuge tube and is also adaptable to centrifuge tubes with different hole diameters.

[0019] Preferably, the centrifuge tube rack is marked with positioning lines.

[0020] Compared with the prior art, the beneficial effects of the present invention are:

[0021] 1. This plant sampling device features a base with a U-shaped placement groove, and a sliding groove on top of the U-shaped placement groove that matches the centrifuge tube rack. A fixing frame and a punching device are installed above the U-shaped placement groove. Before sampling, centrifuge tubes can be placed on the centrifuge tube rack, and plant tissues such as leaves can be placed on top of the centrifuge tubes. By pressing the punching device, holes can be punched in the plant tissues to extract the sample. The extracted plant tissue falls directly into the centrifuge tube without manual transfer, making the operation convenient and quick. Multiple sampling operations can also be performed simultaneously by moving the centrifuge tube rack, further improving efficiency.

[0022] 2. The perforation device of this sampling apparatus includes a limiting cylinder, connecting tube, limiting block, perforating knife, pressing rod, pressing block, spring, and other structures. During sampling, the limiting cylinder moves down to press the plant tissue such as leaves, and then the perforating knife punches holes in the plant tissue to collect the sample. No manual pressing of the leaf tissue is required, making operation simple. This sampling apparatus can also be equipped with two or more perforation devices simultaneously, allowing for simultaneous sampling of two or more samples per operation, more than doubling the sampling efficiency.

[0023] 3. The sampling device has an elastic pushing block inside the punching blade. When the punching blade samples the leaf tissue, the pushing block pushes the excised leaf tissue outward into the centrifuge tube, further improving sampling efficiency. Simultaneously, the pushing block is fixed to the punching blade by a spring, providing a certain degree of elasticity and preventing damage to the leaf tissue. Attached Figure Description

[0024] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below.

[0025] Figure 1 This is a schematic diagram of the plant tissue sampling device of the present invention.

[0026] Figure 2 This is a schematic diagram of the disassembled structure of the plant tissue sampling device of the present invention.

[0027] Figure 3 This is a schematic diagram of the connection structure of the punching device of the present invention.

[0028] Figure 4 This is a cross-sectional view of the punching device of the present invention.

[0029] Figure 5 This is a schematic diagram of another embodiment of the present invention.

[0030] Figure 6 This is a cross-sectional view of a drilling device according to another embodiment of the present invention.

[0031] The markings shown in the figure are:

[0032] 10. Base; 11. U-shaped placement groove; 111. Slide groove;

[0033] 20. Centrifuge tube rack; 21. Centrifuge tube placement hole; 22. Positioning line;

[0034] 30. Fixing bracket; 31. Fixing horizontal bar; 32. Fixing vertical bar; 33. Fixing hole; 34. Fixing groove;

[0035] 40. Drilling device; 41. Limiting cylinder; 42. Connecting pipe; 43. Limiting block; 44. Drilling knife; 45. Pressing rod; 46. Pressing block; 47. Spring; 48. Pushing block. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0037] In the description of this invention, it should be noted that the terms "inner" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the invention is usually placed when in use. They are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the invention.

[0038] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0039] Example 1

[0040] like Figure 1-4 As shown, a plant tissue sampling device includes:

[0041] The base 10 has a U-shaped placement groove 11, and sliding grooves 111 are respectively provided on the upper parts of both sides of the U-shaped placement groove 11. The sliding grooves 111 are horizontally arranged. In this embodiment, there are two U-shaped placement grooves 11, and the two U-shaped placement grooves 11 can be set to the same size or different sizes.

[0042] Centrifuge tube rack 20, with both sides of the centrifuge tube rack 20 mounted on the slide groove 111 and capable of sliding horizontally relative to the slide groove 111, centrifuge tube rack 20 is provided with centrifuge tube placement holes 21 at intervals. The centrifuge tube rack 20 and centrifuge tube placement holes 21 can be provided in various ways to meet the placement requirements of centrifuge tubes of different specifications.

[0043] A fixing frame 30 is disposed above the base 10 and the centrifuge tube rack 20, and the fixing frame 30 is provided with fixing holes 33. In this embodiment, the fixing frame 30 includes a fixing horizontal bar 31 and a fixing vertical bar 32, and the fixing holes 33 are provided on the fixing horizontal bar 31. The two ends of the fixing horizontal bar 31 are respectively fixed to the outer side of the U-shaped placement groove 11 through the fixing vertical bar 32. Further, there are two fixing holes 33. Of course, more fixing holes 33 can also be provided.

[0044] A drilling device 40 is mounted on the fixing frame 30 and passes through the fixing hole 33. In this embodiment, there are two drilling devices 40.

[0045] In use, insert the centrifuge tubes into the centrifuge tube placement holes 21 of the centrifuge tube rack 20 and fix them in place. Then, insert the centrifuge tube rack 20 into the sliding groove 111 of the U-shaped placement slot 11. Place the leaf to be sampled above the centrifuge tube opening. Push the centrifuge tube rack 20 with one hand so that the centrifuge tube opening is below the punching device 40. During the movement, lift the punching device 40 with the other hand. After the centrifuge tube rack 20 is moved to the designated position, press down on the punching device 40 to punch a hole in the leaf for sampling. The sampled leaf tissue falls into the centrifuge tube. The operation is simple and quick. Continue to move the centrifuge tube rack 20 to sample other parts of the leaf.

[0046] Specifically, the drilling device 40 includes a limiting cylinder 41, a connecting pipe 42, a limiting block 43, a drilling knife 44, a pressing rod 45, and a pressing block 46. The connecting pipe 42 is a hollow structure that passes through the fixing hole 33. One end of the connecting pipe 42 is connected to the limiting cylinder 41, and the other end is connected to the limiting block 43. The limiting block 43 has a through hole located above the fixing hole 33. The bottom of the limiting cylinder 41... The tube has an opening at the top and a through hole. The pressing rod 45 is located inside the connecting tube 42 and passes through the through hole of the limiting block 43 and the through hole of the limiting cylinder 41. One end of the pressing rod 45 is connected to the punching knife 44, and the other end is connected to the pressing block 46. The punching knife 44 is located inside the limiting cylinder 41. A spring 47 is also sleeved on the outer periphery of the pressing rod 45, and the spring 47 is located between the pressing block 46 and the limiting block 43. That is, there is a certain distance between the pressing block 46 and the limiting block 43. During the punching operation, the pressing block 46 is pressed down, that is, the punching knife 44 punches and samples the blade tissue. In other embodiments, the spring 47 can also be located between the punching knife 44 and the limiting cylinder 41. The setting of the limiting cylinder 41 not only protects the punching knife 44, but also presses the blade tissue during the punching operation, which is more conducive to the punching operation.

[0047] Specifically, the punching knife 44 is a hollow cylinder with one open end, and its cutting edge is serrated. Further, the end of the pressing rod 45 has a threaded mounting hole, and the top of the punching knife 44 is fixed with a threaded connecting part. The punching knife 44 is detachably connected to the pressing rod 45 through the connecting part. The height of the punching knife 44 is less than the height of the limiting cylinder 41. That is, during the punching operation, the limiting cylinder 41 first contacts the blade tissue, presses the blade tissue down, and then the punching knife 44 punches and samples the blade, which is more conducive to the extraction of the blade tissue. Preferably, a rubber ring or similar material can be fitted onto the bottom of the limiting cylinder 41, which can increase the contact area with the blade tissue and prevent the limiting cylinder 41 from damaging the blade tissue.

[0048] Preferably, the punching device 40 further includes a pushing block 48, which is connected to the inner top of the punching blade 44 via a spring 47, and the end of the pushing block 48 is flush with the cutting edge of the punching blade 44. That is, while the punching blade 44 punches and samples the leaf tissue, the pushing block 48 pushes the extracted leaf tissue into the centrifuge tube, which helps to improve the sampling efficiency of the leaf tissue.

[0049] Further, a fixing groove 34 is provided on the fixing frame 30, the fixing hole 33 is arranged in the fixing groove 34, the shape and size of the fixing groove 34 match the limiting block 43 of the punching device 40, the limiting block 43 is arranged at the fixing groove 34, and an elastic member is provided between the bottom of the limiting block 43 and the bottom of the fixing groove 34, and the elastic force of the elastic member is less than the elastic force of the spring 47 on the outer periphery of the pressing rod 45. That is, before pressing the punching device 40, there is a certain distance between the bottom of the limiting cylinder 41 of the punching device 40 and the centrifuge tube rack 20. When the centrifuge tube rack 20 and the leaf tissue move to the designated position, the punching device 40 is pressed, the elastic member in the fixing groove 34 is compressed, the whole punching device 40 moves downward, and the bottom of the limiting cylinder 41 contacts the leaf tissue to fix the leaf tissue; continue to press down the punching device 40, the spring 47 on the outer periphery of the pressing rod 45 contracts, and the punching knife 44 punches and samples the leaf tissue.

[0050] Further, two punching devices 40 are mounted on the fixing frame 30. The two punching devices 40 are respectively located above the two U-shaped placement grooves 11. The sizes of the punching knives 44 supporting the two punching devices 40 are different. Correspondingly, the apertures of the centrifuge tube placement holes 21 on the two centrifuge tube racks 20 are different. Different punching devices 40 and centrifuge tubes can be selected according to needs. Of course, the punching knife 44 and the centrifuge tube rack 20 can also be replaced according to needs.

[0051] Further, positioning lines 22 are drawn on the centrifuge tube rack 20. It is beneficial to the positioning of the centrifuge tube during the operation. In other embodiments, an infrared sensing device can also be provided at the U-shaped placement groove 11. When the centrifuge tube reaches the designated position, a warning sound is emitted to prompt the operator to stop pushing the centrifuge tube rack 20.

[0052] Embodiment 2

[0053] As Figure 5-6 shown, a plant tissue sampling device includes:

[0054] A base 10, on which a U-shaped placement groove 11 is provided, and upper parts of both sides of the U-shaped placement groove 11 are respectively provided with sliding grooves 111, and the sliding grooves 111 are horizontally arranged.

[0055] A centrifuge tube rack 20, both sides of the centrifuge tube rack 20 are mounted at the sliding grooves 111 and can slide horizontally relative to the sliding grooves 111, and centrifuge tube placement holes 21 are arranged at intervals on the centrifuge tube rack 20. The centrifuge tube rack 20 can be matched with various specifications and can meet the requirements of different plant genome extraction tests.

[0056] A fixing frame 30 is disposed above the base 10 and the centrifuge tube rack 20, and the fixing frame 30 is provided with fixing holes 33. In this embodiment, the fixing frame 30 includes a fixing horizontal bar 31 and a fixing vertical bar 32. The fixing holes 33 are provided on the fixing horizontal bar 31, and the two ends of the fixing horizontal bar 31 are respectively fixed to the outside of the U-shaped placement groove 11 through the fixing vertical bar 32. In this embodiment, the fixing horizontal bar 31 is also provided with a sliding groove, and the fixing holes 33 are spaced apart on the sliding groove, so that the installation position of the drilling device 40 can be selected as needed.

[0057] A drilling device 40 is mounted on the fixing frame 30 and passes through the fixing hole 33. In this embodiment, there is one drilling device 40. The drilling device 40 can move along the sliding groove and be installed in a suitable fixing hole 33.

[0058] The operation method of this embodiment is similar to that of Embodiment 1. In use, the centrifuge tubes are inserted into the centrifuge tube placement holes 21 of the centrifuge tube rack 20 and fixed in place. The centrifuge tube rack 20 is then inserted into the sliding groove 111 of the U-shaped placement slot 11. The leaf to be sampled is placed above the centrifuge tube opening. One hand pushes the centrifuge tube rack 20 so that the centrifuge tube opening is below the punching device 40. During the movement, the other hand lifts the punching device 40. After the centrifuge tube rack 20 moves to the designated position, the punching device 40 is pressed down, causing it to punch a hole in the leaf for sampling. The sampled leaf tissue falls into the centrifuge tube. The operation is simple and quick. The centrifuge tube rack 20 is then moved further to sample other parts of the leaf.

[0059] Specifically, the drilling device 40 includes a limiting cylinder 41, a connecting pipe 42, a limiting block 43, a drilling knife 44, a pressing rod 45, and a pressing block 46. The connecting pipe 42 is a hollow structure that passes through the fixing hole 33. One end of the connecting pipe 42 is connected to the limiting cylinder 41, and the other end is connected to the limiting block 43. The limiting block 43 has a through hole located above the fixing hole 33. The bottom of the limiting cylinder 41... The device has an opening at the top and a through hole. The pressing rod 45 is located inside the connecting tube 42 and passes through the through hole of the limiting block 43 and the through hole of the limiting cylinder 41. One end of the pressing rod 45 is connected to the punching knife 44, and the other end is connected to the pressing block 46. The punching knife 44 is located inside the limiting cylinder 41. A spring 47 is also sleeved on the outer periphery of the pressing rod 45, and the spring 47 is located between the pressing block 46 and the limiting block 43. In this embodiment, there are two punching devices 40, and the pressing blocks 46 of the two punching devices 40 are connected to form a large pressing block 46. That is, pressing one pressing block 46 can operate two punching devices 40 at the same time. Of course, in other embodiments, more punching devices 40 can be set to further improve efficiency. In other embodiments, an elastic gasket is provided inside the centrifuge tube placement hole 21, which is not only conducive to the placement of centrifuge tubes, but also adaptable to centrifuge tubes with different hole diameters.

[0060] Specifically, the punching knife 44 is a hollow cylinder with one open end, and its cutting edge is serrated. This facilitates cutting out the blade. Furthermore, the end of the pressing rod 45 has a threaded mounting hole, and the top of the punching knife 44 is fixed with a threaded connecting part. The punching knife 44 is detachably connected to the pressing rod 45 through the connecting part. The height of the punching knife 44 is less than the height of the limiting cylinder 41. That is, during the punching operation, the limiting cylinder 41 first contacts the blade tissue, pressing it down, and then the punching knife 44 punches and samples the blade, which is more conducive to the removal of the blade tissue. A rubber ring can be fitted onto the bottom of the limiting cylinder 41, which increases the contact area with the blade tissue and prevents the limiting cylinder 41 from damaging the blade tissue.

[0061] Preferably, the punching device 40 further includes a pushing block 48, which is connected to the inner top of the punching blade 44 via a spring 47. The pushing block 48 is located in the middle of the inner side of the punching blade 44, and its end is flush with the cutting edge of the punching blade 44. That is, while the punching blade 44 is punching and sampling the leaf tissue, the pushing block 48 pushes the extracted leaf tissue into the centrifuge tube, which helps to improve the sampling efficiency of the leaf tissue.

[0062] Furthermore, the fixing frame 30 is provided with a fixing groove 34, and the fixing hole 33 is provided in the fixing groove 34. The shape and size of the fixing groove 34 match the limiting block 43 of the punching device 40. The limiting block 43 is located at the fixing groove 34, and an elastic element is provided between the bottom of the limiting block 43 and the bottom of the fixing groove 34. The elastic force of the elastic element is less than the elastic force of the spring 47 on the outer periphery of the pressing rod 45. That is, before pressing the punching device 40, the bottom of the limiting cylinder 41 of the punching device 40 is a certain distance away from the centrifuge tube rack 20. When the centrifuge tube rack 20 and the blade tissue move to the designated position, pressing the punching device 40 compresses the elastic element in the fixing groove 34, causing the punching device 40 to move down as a whole, and the bottom of the limiting cylinder 41 to contact the blade tissue to fix the blade tissue; continuing to press the punching device 40 down causes the spring 47 on the outer periphery of the pressing rod 45 to contract, and the punching knife 44 punches and samples the blade tissue.

[0063] Furthermore, positioning lines 22 are drawn on the centrifuge tube rack 20. This facilitates the positioning of the centrifuge tubes during operation. In other embodiments, a positioning strip with a certain elasticity can also be provided inside the U-shaped placement groove 11 for positioning, or an elastic protrusion and groove can be provided between the centrifuge tube rack 20 and the U-shaped placement groove 11 for positioning. After a certain external force is applied, the protrusion can move relative to the groove.

[0064] This device is used for leaf tissue sampling in plant genome extraction experiments. Before sampling, centrifuge tubes are inserted into the centrifuge tube placement holes 21 of the centrifuge tube rack 20 and fixed in place. The centrifuge tube rack 20 is then inserted into the sliding groove 111 of the U-shaped placement slot 11. The leaf to be sampled is placed above the centrifuge tube opening. One hand pushes the centrifuge tube rack 20 so that the centrifuge tube opening is below the punching device 40. After the centrifuge tube rack 20 moves to the designated position, the punching device 40 is pressed down. The bottom of the limiting cylinder 41 first contacts the leaf tissue to fix it. The punching device 40 is pressed down further, and the spring 47 on the outer periphery of the pressing rod 45 retracts. The punching knife 44 punches holes in the leaf tissue to extract the sample. At the same time, the pushing block 48 pushes the extracted leaf tissue into the centrifuge tube. The operation is simple and quick. The centrifuge tube rack 20 is then moved to sample other parts of the leaf. This embodiment is equipped with two punching devices 40, which can sample two parts at the same time with each operation, doubling the sampling efficiency.

[0065] In summary, this sampling device features a base 10 with a U-shaped placement groove 11, and a sliding groove 111 matching the centrifuge tube rack 20 on the upper part of the U-shaped placement groove 11. A fixing frame 30 and a punching device 40 are installed above the U-shaped placement groove 11. Before sampling, centrifuge tubes can be placed in the centrifuge tube placement groove on the centrifuge tube rack 20, and plant tissues such as leaves can be placed on top of the centrifuge tubes. By pressing the punching device 40, the plant tissues such as leaves can be punched for sampling, and the extracted plant tissues fall directly into the centrifuge tubes without manual transfer, making the operation convenient and quick. Multiple sampling operations can also be performed simultaneously by moving the centrifuge tube rack 20, further improving efficiency. This device can be equipped with centrifuge tube racks 20 of different specifications, as well as punching devices 40 of different specifications, to meet the sampling requirements of different centrifuge tubes in different experiments. It can also be equipped with different numbers of punching devices 40 to further improve sampling efficiency.

[0066] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A plant tissue sampling device, characterized in that, It includes: A base, wherein a U-shaped placement groove is provided on the base, and a sliding groove is provided on the upper part of the U-shaped placement groove, and the sliding groove is horizontally arranged; Centrifuge tube rack, with both sides of the centrifuge tube rack mounted on the slide groove and able to slide horizontally relative to the slide groove, and centrifuge tube placement holes are provided at intervals on the centrifuge tube rack; A fixing frame is provided above the base and the centrifuge tube rack, and the fixing frame is provided with fixing holes; A drilling device is mounted on a fixed frame and passes through a fixed hole. The drilling device includes a limiting cylinder, a connecting tube, a limiting block, a drilling knife, a pressing rod, and a pressing block. The connecting tube is hollow and passes through the fixed hole. One end of the connecting tube is connected to the limiting cylinder, and the other end is connected to the limiting block. The limiting block has a through hole located above the fixed hole. The limiting cylinder has an opening at the bottom and a through hole at the top. The pressing rod is located inside the connecting tube and passes through the through hole in the limiting block and the through hole in the limiting cylinder. One end of the pressing rod is connected to the drilling knife, and the other end is connected to the pressing block. The drilling knife is located inside the limiting cylinder. A spring is also sleeved on the outer periphery of the pressing rod. The spring is located between the pressing block and the limiting block, or between the drilling knife and the limiting cylinder. The punching device also includes a pressing block, which is connected to the inner side of the top of the punching knife by a spring, and the end of the pressing block is flush with the blade of the punching knife; The height of the punching tool is less than the height of the limiting cylinder; The fixing frame is provided with a fixing groove, and the fixing hole is provided in the fixing groove. The shape and size of the fixing groove match the limiting block of the punching device. The limiting block is provided at the fixing groove, and an elastic element is provided between the bottom of the limiting block and the bottom of the fixing groove. The elastic force of the elastic element is less than the elastic force of the spring on the outer periphery of the pressing rod. The mounting frame is equipped with multiple punching devices; the U-shaped placement slot and the centrifuge tube rack are also equipped with multiple devices.

2. The plant tissue sampling device according to claim 1, characterized in that, The punch is a hollow cylinder with one open end, and the cutting edge of the punch is serrated.

3. The plant tissue sampling device according to claim 2, characterized in that, The end of the pressing rod is provided with an internally threaded mounting hole, and the top of the punching tool is fixed with an externally threaded connecting part. The punching tool is detachably connected to the pressing rod through the connecting part.

4. The plant tissue sampling device according to claim 1, characterized in that, An elastic gasket is provided inside the centrifuge tube placement hole.

5. The plant tissue sampling device according to claim 1, characterized in that, Positioning lines are drawn on the centrifuge tube rack.

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