Methods, systems, devices, equipment and media for monitoring liquid injection

Abstract

This application relates to a method, system, apparatus, device, and medium for monitoring liquid injection. The monitoring method acquires optical data of the reaction liquid in the reaction chamber during the injection of the reaction liquid; based on changes in the optical data, it monitors whether the injection process is normal. The liquid injection monitoring method provided by this application can monitor the injection process without waiting for the PCR chain reaction to complete, thus enabling timely monitoring and reducing monitoring time.

Description

Technical Field

[0001] This application relates to the field of data processing technology, and in particular to a method, system, apparatus, device, and medium for monitoring liquid injection. Background Technology

[0002] Quantitative real-time PCR (Polymerase Chain Reaction) analyzers are very common instruments. Reactants are typically introduced into the reaction chamber of the PCR analyzer using microfluidic technology. However, several factors can cause the reactants to fail to be successfully injected into the reaction chamber, leading to PCR chain reaction failure. These include insufficient sample volume, blockage or leakage in the sample flow channel of the PCR analyzer cartridge, and insufficient pressure preventing sample flow.

[0003] In traditional techniques, the success of reactant injection into the reaction chamber can be determined through internal and external controls within the reaction components. However, this traditional method only allows for assessment after the PCR chain reaction is complete, which is time-consuming. Summary of the Invention

[0004] Therefore, it is necessary to provide a method, system, device, equipment, and medium for monitoring liquid injection to address the aforementioned technical problems.

[0005] In a first aspect, one embodiment of this application provides a method for monitoring liquid injection, the monitoring method comprising:

[0006] During the process of injecting the reaction liquid into the reaction chamber, optical data of the reaction liquid in the reaction chamber is acquired;

[0007] The process of injecting the reaction liquid into the reaction chamber is monitored to ensure it is proceeding normally, based on changes in optical data.

[0008] In one embodiment, monitoring the injection of the reaction liquid into the reaction chamber based on changes in optical data includes:

[0009] Determine the slope curve based on optical data;

[0010] Based on the slope curve, monitor whether the process of injecting the reaction liquid into the reaction chamber is normal.

[0011] In one embodiment, monitoring the injection of the reaction liquid into the reaction chamber based on the slope curve includes:

[0012] Determine whether a target curve exists that matches the reference slope curve;

[0013] If the target curve exists in the slope curve, then the process of injecting the reaction liquid into the reaction chamber is normal.

[0014] In one embodiment, determining the slope curve based on changes in optical data includes:

[0015] The optical data is smoothed according to the preset data processing window to obtain smoothed optical data; the data processing window is used to limit the amount of optical data to be processed.

[0016] The smoothed optical data is fitted with a straight line according to the data processing window to obtain multiple fitted straight lines.

[0017] Calculate the slope of each fitted line, and determine the slope curve based on the slope.

[0018] In one embodiment, the monitoring method further includes:

[0019] If the target curve is not present in the slope curve, then the process of liquid injection into the reaction chamber is abnormal.

[0020] In one embodiment, the monitoring method further includes:

[0021] If it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal, the type of abnormality in the process of injecting the reaction liquid into the reaction chamber can be determined based on the slope curve.

[0022] In one embodiment, the anomaly type of the process of injecting the reaction liquid into the reaction chamber is determined based on the slope curve, including:

[0023] If there are points in the slope curve where the slope is less than zero, then the anomaly type is determined to be liquid reflux.

[0024] Secondly, one embodiment of this application provides a liquid injection monitoring system, which includes: a control device and a polymerase chain reaction (PCR) detector;

[0025] A polymerase chain reaction (PCR) analyzer is used to detect optical data of the reaction liquid in the reaction chamber during the injection of the reaction liquid into the reaction chamber, and to send the optical data to the control device.

[0026] Control equipment is used to monitor whether the process of injecting the reaction liquid into the reaction chamber is normal, based on changes in optical data.

[0027] In one embodiment, the polymerase chain reaction (PCR) analyzer includes an optical detection unit and a cartridge. The optical detection unit is provided with an optical channel, and the cartridge is provided with a reaction chamber.

[0028] A control device is used to control the movement of the optical detection unit before the reaction liquid is injected into the reaction chamber so that the optical channel is aligned with the reaction chamber; and to control the position of the optical detection unit to return to its original position after the reaction liquid is injected into the reaction chamber.

[0029] In one embodiment, the control device is further configured to control the optical detection unit to detect optical data and acquire optical data from the optical detection unit during the injection of the reaction liquid into the reaction chamber.

[0030] Thirdly, one embodiment of this application provides a liquid injection monitoring device, the monitoring device comprising:

[0031] The acquisition module is used to acquire optical data of the reaction liquid in the reaction chamber during the process of injecting the reaction liquid into the reaction chamber;

[0032] The monitoring module is used to monitor whether the process of injecting the reaction liquid into the reaction chamber is normal based on the changes in the optical data.

[0033] Fourthly, one embodiment of this application provides a computer device, including a memory and a processor. The memory stores a computer program, and the processor executes the computer program to implement the steps of the monitoring method provided in the above embodiment.

[0034] Fifthly, one embodiment of this application provides a computer-readable storage medium having a computer program stored thereon, wherein the computer program, when executed by a processor, implements the steps of the monitoring method provided in the above embodiments.

[0035] Sixthly, one embodiment of this application provides a computer program product, including a computer program that, when executed by a processor, implements the steps of the monitoring method provided in the above embodiments.

[0036] This application provides a method, system, apparatus, device, and medium for monitoring liquid injection. The monitoring method acquires optical data of the reaction liquid in the reaction chamber during the injection process; and monitors whether the injection process is normal based on changes in the optical data. The monitoring method provided by this application does not require waiting for the PCR chain reaction to complete when monitoring the injection process, thus enabling timely monitoring and reducing monitoring time. Attached Figure Description

[0037] To more clearly illustrate the technical solutions in the embodiments of this application or the conventional technology, the drawings used in the description of the embodiments or the conventional technology will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0038] Figure 1 A schematic diagram illustrating the variation of optical data provided in one embodiment;

[0039] Figure 2 A flowchart illustrating the steps of a liquid injection monitoring method provided in one embodiment;

[0040] Figure 3 A flowchart illustrating the steps of a liquid injection monitoring method provided in another embodiment;

[0041] Figure 4 A flowchart illustrating the steps of a liquid injection monitoring method provided in another embodiment;

[0042] Figure 5 A schematic diagram illustrating the variation of optical data provided for another embodiment;

[0043] Figure 6 A schematic diagram of a slope curve provided for one embodiment;

[0044] Figure 7 A flowchart illustrating the steps of a liquid injection monitoring method provided in another embodiment;

[0045] Figure 8 A schematic diagram illustrating the variation of optical data provided for another embodiment;

[0046] Figure 9 A schematic diagram of the slope curve provided for another embodiment;

[0047] Figure 10 A schematic diagram of the structure of a liquid injection monitoring system provided in one embodiment;

[0048] Figure 11 A schematic diagram of the structure of a liquid injection monitoring system provided for another embodiment;

[0049] Figure 12 A schematic diagram of the structure of a liquid injection monitoring system provided for another embodiment;

[0050] Figure 13 A schematic diagram of the optical detection unit after it has been moved, provided in one embodiment;

[0051] Figure 14 A schematic diagram of the structure of a liquid injection monitoring device provided in one embodiment;

[0052] Figure 15 This is a schematic diagram of the structure of a computer device provided in one embodiment of this application. Detailed Implementation

[0053] To make the above-mentioned objectives, features, and advantages of this application more apparent and understandable, the specific embodiments of this application are described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of this application. However, this application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this application. Therefore, this application is not limited to the specific embodiments disclosed below.

[0054] First, before introducing the technical solutions of the embodiments of this disclosure in detail, the technical background or evolution of the embodiments of this disclosure will be introduced. A quantitative PCR (Polymerase Chain Reaction) detector refers to an instrument that uses fluorescent dyes or fluorescently labeled specific probes to label and track the products of a PCR chain reaction and monitor the reaction process in real time. Multiple reasons, such as blockage or leakage of the sample flow channel in the PCR detector cartridge, or insufficient pressure preventing sample flow, can cause the reactants to fail to be successfully injected into the PCR reaction chamber, leading to the failure of the PCR chain reaction. Therefore, determining how successfully the reactants are injected into the reaction chamber is a challenging problem.

[0055] In traditional techniques, the internal and external controls within the PCR chain reaction products can be used to determine the reactants required for PCR, thus confirming whether the reactants have been successfully injected into the reaction chamber. However, this traditional method only verifies successful injection after the PCR chain reaction is complete, which is time-consuming. To address this, this application provides a liquid injection monitoring method and system. The reactants contain various chemical components (such as specific probes), and after passing through a series of filters, they exhibit a clear optical signal before amplification. Even if the chemical components in the reactants do not have a clear optical signal, because the reactants are liquid, they will still produce weak optical signal changes during injection into the reaction chamber. Specifically, the changes in the optical data of the reaction liquid in the reaction chamber during injection are as follows: Figure 1 As shown, Figure 1 The horizontal axis represents time, and the vertical axis represents optical data (light intensity). From Figure 1 As can be seen, the optical data of the reaction liquid in the reaction chamber also changes over time. Therefore, by detecting the optical changes during the injection of the reaction liquid into the reaction chamber, the entire process of the reaction liquid flowing into the reaction chamber can be monitored.

[0056] The technical solution of this application and how it solves the technical problems will be described in detail below with specific embodiments. These specific embodiments can be combined with each other, and the same or similar concepts or processes may not be described again in some embodiments. The embodiments of this application will be described below with reference to the accompanying drawings.

[0057] Please see Figure 2 This application provides a method for monitoring liquid injection, illustrated by an example of the method being applied to a control device. The monitoring method includes the following steps:

[0058] Step 200: During the process of injecting the reaction liquid into the reaction chamber, acquire optical data of the reaction liquid in the reaction chamber.

[0059] The control device acquires the optical data (light intensity) of the reaction liquid in the reaction chamber of the PCR detector. The optical data of the reaction liquid in the reaction chamber of the PCR detector can be obtained in real time during the injection of the reaction liquid (reactant) into the reaction chamber. This embodiment does not limit the specific method for acquiring the optical data of the reaction liquid in the reaction chamber, as long as the function can be achieved.

[0060] In one specific embodiment, the optical data can be detected by the PCR detector and stored in the PCR detector's built-in storage device. The control device can then directly retrieve the data from the PCR detector's built-in storage device when needed. Alternatively, the optical data can be detected by the PCR detector, sent to the control device, and stored in the control device's memory. The control device can then directly retrieve the data from the memory when needed.

[0061] Step 210: Monitor whether the process of injecting the reaction liquid into the reaction chamber is normal based on the changes in optical data.

[0062] After obtaining the optical data of the reaction liquid in the reaction chamber, the control equipment can monitor whether the injection process of the reaction liquid into the reaction chamber is normal based on the changes in the optical data. Specifically, the injection process of the reaction liquid into the reaction chamber will cause changes in the optical data of the reaction liquid. The changes in the optical data detected by the PCR detector are used to determine whether the reaction liquid can be successfully injected into the reaction chamber, and the entire process of injection can be monitored. This embodiment does not limit the specific method for monitoring the normality of the reaction liquid injection process based on changes in optical data, as long as the function can be achieved.

[0063] The liquid injection monitoring method provided in this application acquires optical data of the reaction liquid being injected into the reaction chamber during the process. Based on changes in the optical data, the method monitors whether the liquid injection process is normal. This monitoring method does not require waiting for the PCR chain reaction to complete, enabling timely monitoring and reducing monitoring time. Furthermore, by using changes in optical data, the method can monitor the normality of the liquid injection process, improving its practicality and reliability.

[0064] Please see Figure 3 In one embodiment, one possible implementation involves monitoring whether the process of injecting a reaction liquid into a reaction chamber is normal based on changes in optical data, the implementation comprising the steps of:

[0065] Step 300: Determine the slope curve based on the optical data.

[0066] After obtaining optical data, the control device determines the corresponding slope curve based on the optical data. The slope curve can be used to represent the changes in the optical data. This embodiment does not limit the specific method for determining the slope curve based on the optical data, as long as the function can be achieved.

[0067] Step 310: Based on the slope curve, monitor whether the process of injecting the reaction liquid into the reaction chamber is normal.

[0068] After obtaining the slope curve, the control equipment can monitor whether the injection of the reaction liquid into the reaction chamber is normal. In other words, by observing the changes in the slope curve, the changes in the optical data of the reaction liquid in the reaction chamber during the injection process can be determined, thereby confirming whether the reaction liquid has been successfully injected into the reaction chamber.

[0069] In an optional embodiment, the magnitude of the slope in the slope curve can indicate whether the optical data has changed, and how fast the optical data changes. Specifically, if the slope of the slope curve is zero, it indicates that the optical data has not changed; if the slope of the slope curve is larger, it indicates that the optical data changes faster.

[0070] In this embodiment, the control device monitors whether the process of injecting the reaction liquid into the reaction chamber is normal by using the slope curve of the optical data. This avoids the slow change of the optical data of the liquid in the reaction chamber under pressure when no reaction liquid is injected into the reaction chamber, which may affect the monitoring of whether the process of injecting the reaction liquid into the reaction chamber is normal. This improves the accuracy of monitoring whether the process of injecting the reaction liquid into the reaction chamber is normal.

[0071] Please see Figure 4 In one embodiment, a possible implementation involves monitoring whether the process of injecting the reaction liquid into the reaction chamber is normal based on a slope curve, the implementation comprising the steps of:

[0072] Step 400: Determine whether there exists a target curve that matches the reference slope curve.

[0073] The reference slope curve can be a slope curve corresponding to the optical data when the reaction liquid is injected into the reaction chamber normally, as preset by the user. The reference slope curve can be pre-stored in the memory of the control device. After obtaining the slope curve corresponding to the optical data, the control device matches the slope curve with the reference slope curve to determine if there is a target curve that matches the reference slope curve.

[0074] Step 410: If the target curve exists in the slope curve, then the process of injecting the reaction liquid into the reaction chamber is normal.

[0075] After the control equipment matches the slope curve with the reference slope curve, if it determines that there is a target curve in the slope curve that matches the reference slope curve, it can be determined that the process of injecting the reaction liquid into the reaction chamber is normal, that is, the reaction liquid has been successfully injected into the reaction chamber.

[0076] In one embodiment, the monitoring method for liquid injection further includes the following steps:

[0077] If the target curve is not present in the slope curve, it indicates that the process of injecting the reaction liquid into the reaction chamber is abnormal.

[0078] If the control equipment determines that there is no target curve in the slope curve that matches the reference slope curve after matching the slope curve with the reference slope curve, then it can be determined that the process of injecting the reaction liquid into the reaction chamber is abnormal, that is, there is an abnormal situation in the process of injecting the reaction liquid into the reaction chamber.

[0079] In an optional embodiment, the control device can calculate the similarity between the slope curve and a reference slope curve, and determine whether a target curve exists in the slope curve by comparing the similarity with a preset similarity threshold. If the control device determines that the similarity is greater than or equal to the preset similarity threshold, it determines that a target curve exists in the slope curve; if the control device determines that the similarity is less than the preset similarity threshold, it determines that a target curve does not exist in the slope curve.

[0080] In this embodiment, by matching the slope curve with the reference slope curve, the presence of the target curve in the slope curve can be quickly and accurately determined, thereby improving the efficiency of determining whether the process of injecting the reaction liquid into the reaction chamber is normal.

[0081] In one embodiment, when the reaction liquid is successfully injected into the reaction chamber, the changes in the optical data of the reaction liquid in the reaction chamber are as follows: Figure 5 As shown, Figure 5 The horizontal axis represents time (ms), and the vertical axis represents optical data (light intensity). From Figure 5 As can be seen, the optical data increases significantly at approximately 8000 ms, indicating that the reactive liquid begins to be injected into the reaction chamber at this point. The slope curve corresponding to the optical data of the reactive liquid in the reaction chamber is shown in the figure. Figure 6 As shown, Figure 6 The horizontal axis represents time, and the vertical axis represents the slope. From Figure 6 As can be seen, at approximately 8000 ms, the slope of the optical data begins to increase, indicating that the reaction liquid begins to be injected into the reaction chamber; at approximately 11000 ms, the slope of the optical data gradually approaches zero, indicating that the reaction liquid stops being injected into the reaction chamber, that is, the reaction liquid has been successfully injected into the reaction chamber.

[0082] Please see Figure 7 In one embodiment, one possible implementation involves determining a slope curve based on changes in optical data, the steps of which include:

[0083] Step 700: Smooth the optical data according to the preset data processing window to obtain smoothed optical data; the data processing window is used to limit the amount of optical data to be processed.

[0084] The preset data processing window can be determined by the user based on the sampling frequency and fluctuation characteristics of the optical data (the characteristics of the amplitude of change in the optical data) when the PCR detector detects optical data. Furthermore, the preset data processing window can be pre-stored by the user in the memory of the control device, which can then retrieve it directly from the memory when needed. This embodiment does not limit the preset data processing window, as long as it can achieve its function.

[0085] After obtaining the optical data, the control device smooths the data according to a preset data processing window to obtain smoothed optical data. Smoothing the optical data reduces the influence of other noise, thereby improving the accuracy of the final determined slope curve.

[0086] Step 710: Perform linear fitting on the smoothed optical data according to the data processing window to obtain multiple fitted lines.

[0087] After obtaining the smoothed optical data, the control device performs point-by-point linear fitting on the smoothed optical data according to a preset data processing window, resulting in multiple fitted lines. The number of fitted lines is the same as the number of data processing windows. This embodiment does not limit the specific method of linear fitting, as long as it achieves the function.

[0088] Step 720: Calculate the slope of each fitted line and determine the slope curve based on the slope.

[0089] After obtaining multiple fitted straight lines, the control device calculates the slope of each fitted straight line, and then connects the slopes of each fitted straight line to obtain the slope curve of the optical data.

[0090] In this embodiment, the optical data is first smoothed, and then a straight line fitting process is performed to obtain multiple fitted straight lines. By calculating the slope of each fitted straight line, the slope curve of the optical data can be obtained, which can improve the accuracy of the determined slope curve.

[0091] In one embodiment, the monitoring method for liquid injection further includes the following steps:

[0092] If it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal, the type of abnormality in the process of injecting the reaction liquid into the reaction chamber can be determined based on the slope curve.

[0093] If the control device determines an anomaly during the injection of the reaction liquid into the reaction chamber based on the slope curve of the optical data, the control device can analyze the slope curve to determine the type of anomaly in the injection process. This embodiment does not limit the specific method used to determine the type of anomaly in the injection process using the slope curve, as long as the function is achieved.

[0094] In this embodiment, when the process of injecting the reaction liquid into the reaction chamber is abnormal, the control device can analyze and process the slope curve to determine the type of abnormality in the process of injecting the reaction liquid into the reaction chamber, thus making the liquid injection monitoring method provided in this application practical.

[0095] In one embodiment, one possible implementation involves determining the type of anomaly in the process of injecting the reaction liquid into the reaction chamber based on a slope curve, the implementation comprising the steps of:

[0096] If there are points in the slope curve where the slope is less than zero, then the anomaly type is determined to be liquid reflux.

[0097] The control equipment analyzes the slope curve, specifically determining the anomaly type by identifying any slope points with a slope less than zero. The presence of a slope point less than zero indicates a reversal in the optical data. This means that during the injection of the reaction liquid into the reaction chamber, there was a backflow of the reaction liquid, i.e., the reaction liquid flowed out of the reaction chamber. In other words, the anomaly type during the injection of the reaction liquid into the reaction chamber can be determined to be liquid backflow.

[0098] In this embodiment, the presence of slope points less than zero in the slope curve determines whether the anomaly type is liquid reflux. This method for determining the anomaly type is simple, convenient, and quick.

[0099] In one embodiment, when the process of injecting the reaction liquid into the reaction chamber is abnormal, the changes in the optical data of the reaction liquid in the reaction chamber are as follows: Figure 8 As shown, Figure 8 The horizontal axis represents time (ms), and the vertical axis represents optical data (light intensity). From Figure 8 It can be seen that the changes in optical data are irregular, with repeated increases and decreases. The slope curve corresponding to the optical data of the reaction liquid in the reaction chamber is shown in the figure. Figure 9 As shown, Figure 9 The horizontal axis represents time, and the vertical axis represents the slope. From Figure 9 As can be seen, the slope curve is less than zero after about 5000ms, and it is also less than zero after 15000ms, indicating that there is backflow during the injection of the reaction liquid into the reaction chamber.

[0100] In another embodiment, if all slope points in the slope curve are zero, the anomaly type is determined to be that the reaction liquid has not been injected into the reaction chamber. This anomaly could be caused by insufficient pressure preventing the reaction liquid from being injected into the reaction chamber, or by the reaction liquid flowing in and then flowing out again.

[0101] In another embodiment, if there is no target curve in the slope curve that matches the reference slope curve, but the slope curve shows a trend of gradually increasing to a peak and then gradually decreasing, and this trend lasts for a longer period compared to the reference slope curve, it indicates that the reaction liquid has indeed been injected into the reaction chamber, but an anomaly still exists. This anomaly is that the rate at which the reaction liquid is injected into the reaction chamber is slow. The cause of this anomaly may be a blockage in the channel through which the reaction liquid is injected into the reaction chamber, or a leak in the channel.

[0102] It should be understood that although the steps in the flowcharts of the embodiments described above are shown sequentially according to the arrows, these steps are not necessarily executed in the order indicated by the arrows. Unless explicitly stated herein, there is no strict order restriction on the execution of these steps, and they can be executed in other orders. Moreover, at least some steps in the flowcharts of the embodiments described above may include multiple steps or multiple stages. These steps or stages are not necessarily completed at the same time, but can be executed at different times. The execution order of these steps or stages is not necessarily sequential, but can be performed alternately or in turn with other steps or at least some of the steps or stages of other steps.

[0103] Please see Figure 10 One embodiment of this application provides a liquid injection monitoring system 10, which includes a control device 11 and a polymerase chain reaction (PCR) analyzer 12, which are communicatively connected. Specifically, the control device 11 and the PCR analyzer 12 can be connected wirelessly or via a wired connection. The control device 11 can be a computer, a microprocessor chip, or other device. The computer device can be, but is not limited to, an industrial computer, a laptop computer, a smartphone, a tablet computer, and a portable wearable device.

[0104] The polymerase chain reaction (PCR) detector 12 is used to detect the optical data of the reaction liquid (reactant) in the reaction chamber during the injection of the reaction liquid into the reaction chamber, and to send the optical data to the control device 11. In other words, during the injection of the reaction liquid into the reaction chamber, the PCR detector 12 uses real-time detection of the optical data (light intensity) of the reaction liquid in the reaction chamber after injection. After detecting the optical data, the PCR detector 12 can immediately send it to the control device 11; alternatively, it can store the detected optical data in its own storage device, which the control device 11 can retrieve when needed.

[0105] The control device 11 is used to monitor whether the process of injecting the reaction liquid into the reaction chamber is normal based on changes in optical data. A description of how the control device 11 monitors the process of injecting the reaction liquid into the reaction chamber based on changes in optical data can be found in the detailed description of the liquid injection monitoring method described above, and will not be repeated here.

[0106] The liquid injection monitoring system 10 provided in this embodiment can acquire optical data using the PCR detector itself. The control device can monitor whether the process of injecting the reaction liquid into the reaction chamber is normal based on changes in the optical data. This eliminates the need for external monitoring equipment, reducing the cost of the liquid injection monitoring system. Furthermore, the control device can monitor the process of injecting the reaction liquid into the reaction chamber through changes in optical data, eliminating the need to wait until the PCR chain reaction is complete, thus reducing monitoring time and allowing for timely detection of any abnormalities in the entire process of injecting the reaction liquid into the reaction chamber. This helps prevent PCR chain reaction failure and the waste of manpower and resources.

[0107] Please see Figure 11 and Figure 12 In one embodiment, the polymerase chain reaction (PCR) analyzer 12 includes an optical detection unit 13 and a cartridge 14. The optical detection unit 13 is provided with an optical channel 15, and the cartridge 14 is provided with a reaction chamber 17. The optical detection unit 13 may have multiple optical channels 15, such as... Figure 12 As shown, optical channel 15 includes optical channel 15A and optical channel 15B. The cartridge 14 can have multiple reaction chambers 17, such as... Figure 11 As shown, the reaction chamber 17 in the cartridge 14 includes reaction chamber 17A, reaction chamber 17B, reaction chamber 17C and reaction chamber D.

[0108] The control device 11 is used to control the movement of the optical detection unit 13 before the reaction liquid is injected into the reaction chamber 17 so that the optical channel 15 is aligned with the reaction chamber 17; and to control the position of the optical detection unit 13 to be restored after the reaction liquid is injected into the reaction chamber 17.

[0109] Before the reaction liquid is injected into the reaction chamber 17, the control device 11 controls the optical detection unit 13 to move so that the optical channel 15 in the optical detection unit 13 is aligned with the reaction chamber 17 in the cartridge 14, allowing the optical detection unit 13 to detect the optical data of the reaction liquid in the reaction chamber through the optical channel 15. After the reaction liquid is injected into the reaction chamber 17, the control device 11 controls the optical detection unit 13 to move away from the cartridge 14 and return to its initial position.

[0110] In a specific embodiment, such as Figure 13 As shown, the control device 11 controls the optical detection unit 13 to move, so that the optical channels 15A and 15B are aligned with the reaction chambers 17B and 17C in the cartridge 14, respectively.

[0111] In this embodiment, the control device 11 can control the movement of the optical detection unit 13, thereby improving the practicality and reliability of the liquid injection monitoring system 10.

[0112] In one embodiment, the control device 11 is further configured to control the optical detection unit 13 to detect optical data and acquire optical data from the optical detection unit 13 during the injection of the reaction liquid into the reaction chamber 17. In other words, the control device 11 sends a detection signal to the optical detection unit 13, and the optical detection unit 13 begins to detect optical data based on the detection signal. After the optical detection unit 13 has completed detecting the optical data, it sends the optical data to the control device 11, enabling the control device 11 to acquire the optical data.

[0113] In another embodiment, the control device 11 is also used to control the optical detection unit 13 to stop detecting optical data after the reaction liquid has been injected into the reaction chamber 17.

[0114] In this embodiment, the start and stop of the optical detection unit 13 can be controlled by the control device 11, which can improve the practicality and reliability of the liquid injection monitoring system 10.

[0115] In one specific embodiment, the optical channel 15 may include a beam splitter, a filter, a lens, and an optical sensor. Light emitted from the reaction cavity 17 is transmitted to the optical sensor through the beam splitter, filter, and lens, and optical data can be obtained through the optical sensor.

[0116] Based on the same inventive concept, this application also provides a liquid injection monitoring device for implementing the liquid injection monitoring method described above. The solution provided by this device is similar to the solution described in the above method; therefore, the specific limitations of one or more liquid injection monitoring device embodiments provided below can be found in the limitations of the liquid injection monitoring method described above, and will not be repeated here.

[0117] Please see Figure 14 One embodiment of this application provides a liquid injection monitoring device 20, which includes an acquisition module 21 and a monitoring module 22.

[0118] The acquisition module 21 is used to acquire optical data of the reaction liquid in the reaction chamber during the process of injecting the reaction liquid into the reaction chamber;

[0119] The monitoring module 22 is used to monitor whether the process of injecting the reaction liquid into the reaction chamber is normal based on changes in optical data.

[0120] In one embodiment, the monitoring module 22 includes a determining unit and a monitoring unit. The determining unit is used to determine the slope curve based on optical data; the monitoring unit is used to monitor whether the process of injecting the reaction liquid into the reaction chamber is normal based on the slope curve.

[0121] In one embodiment, the monitoring unit is specifically used to determine whether there is a target curve in the slope curve that matches the reference slope curve; if there is a target curve in the slope curve, it is determined that the process of injecting the reaction liquid into the reaction chamber is normal.

[0122] In one embodiment, the determining unit is specifically used to smooth the optical data according to a preset data processing window to obtain smoothed optical data; the data processing window is used to limit the number of optical data to be processed; the smoothed optical data is subjected to linear fitting according to the data processing window to obtain multiple fitted lines; the slope of each fitted line is calculated, and the slope curve is determined based on the slope.

[0123] In one embodiment, the monitoring module 22 is further configured to determine that the process of injecting the reaction liquid into the reaction chamber is abnormal if the target curve is not present in the slope curve.

[0124] In one embodiment, the monitoring device further includes an anomaly type determination module. The anomaly type determination module is used to determine the type of anomaly in the process of injecting the reaction liquid into the reaction chamber based on a slope curve, if an anomaly is determined in the process.

[0125] In one embodiment, the anomaly type determination module is specifically used to determine the anomaly type as liquid reflux if there are points in the slope curve with a slope less than zero.

[0126] Each module in the aforementioned liquid injection monitoring device can be implemented entirely or partially through software, hardware, or a combination thereof. These modules can be embedded in or independent of the processor in a computer device, or stored in the memory of a computer device as software, so that the processor can call and execute the corresponding operations of each module.

[0127] In one embodiment, a computer device is provided, which may be a terminal, and its internal structure diagram may be as follows: Figure 15As shown, the computer device includes a processor, memory, communication interface, display screen, and input devices connected via a system bus. The processor provides computing and control capabilities. The memory includes non-volatile storage media and internal memory. The non-volatile storage media stores the operating system and computer programs. The internal memory provides an environment for the operation of the operating system and computer programs stored in the non-volatile storage media. The communication interface is used for wired or wireless communication with external terminals; wireless communication can be achieved through Wi-Fi, mobile cellular networks, NFC (Near Field Communication), or other technologies. When executed by the processor, the computer program implements a method for monitoring liquid injection. The display screen can be an LCD screen or an e-ink display screen. The input devices can be a touch layer covering the display screen, buttons, a trackball, or a touchpad mounted on the computer device casing, or an external keyboard, touchpad, or mouse.

[0128] Those skilled in the art will understand that Figure 15 The structure shown is merely a block diagram of a portion of the structure related to the present application and does not constitute a limitation on the computer device to which the present application is applied. Specific computer devices may include more or fewer components than those shown in the figure, or combine certain components, or have different component arrangements.

[0129] In one embodiment, a computer device is provided, including a memory and a processor, wherein the memory stores a computer program, and the processor executes the computer program to perform the following steps:

[0130] During the process of injecting the reaction liquid into the reaction chamber, optical data of the reaction liquid in the reaction chamber is acquired;

[0131] The process of injecting the reaction liquid into the reaction chamber is monitored to ensure it is proceeding normally, based on changes in optical data.

[0132] In one embodiment, the processor, when executing the computer program, also performs the following steps: determining a slope curve based on optical data; and monitoring whether the process of injecting the reaction liquid into the reaction chamber is normal based on the slope curve.

[0133] In one embodiment, when the processor executes the computer program, it further performs the following steps: determining whether there is a target curve in the slope curve that matches the reference slope curve; if there is a target curve in the slope curve, then determining that the process of injecting the reaction liquid into the reaction chamber is normal.

[0134] In one embodiment, when the processor executes the computer program, it further performs the following steps: smoothing the optical data according to a preset data processing window to obtain smoothed optical data; the data processing window is used to limit the amount of optical data to be processed; performing linear fitting on the smoothed optical data according to the data processing window to obtain multiple fitted lines; calculating the slope of each fitted line, and determining the slope curve based on the slope.

[0135] In one embodiment, the processor, when executing the computer program, also performs the following steps: if the target curve is not present in the slope curve, it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal.

[0136] In one embodiment, when the processor executes the computer program, it also performs the following steps: if it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal, it determines the type of abnormality in the process of injecting the reaction liquid into the reaction chamber based on the slope curve.

[0137] In one embodiment, when the processor executes the computer program, it further performs the following steps: if there are points in the slope curve with a slope less than zero, then the anomaly type is determined to be liquid backflow.

[0138] In one embodiment, a computer-readable storage medium is provided having a computer program stored thereon, the computer program performing the following steps when executed by a processor:

[0139] During the process of injecting the reaction liquid into the reaction chamber, optical data of the reaction liquid in the reaction chamber is acquired;

[0140] The process of injecting the reaction liquid into the reaction chamber is monitored to ensure it is proceeding normally, based on changes in optical data.

[0141] In one embodiment, when the computer program is executed by the processor, it also performs the following steps: determining a slope curve based on optical data; and monitoring whether the process of injecting the reaction liquid into the reaction chamber is normal based on the slope curve.

[0142] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: determining whether there is a target curve in the slope curve that matches the reference slope curve; if there is a target curve in the slope curve, determining that the process of injecting the reaction liquid into the reaction chamber is normal.

[0143] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: smoothing the optical data according to a preset data processing window to obtain smoothed optical data; the data processing window is used to limit the amount of optical data to be processed; the smoothed optical data is subjected to linear fitting according to the data processing window to obtain multiple fitted lines; the slope of each fitted line is calculated, and the slope curve is determined based on the slope.

[0144] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: if the target curve is not present in the slope curve, it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal.

[0145] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: if it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal, it determines the type of abnormality in the process of injecting the reaction liquid into the reaction chamber based on the slope curve.

[0146] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: if there are points in the slope curve with a slope less than zero, then the anomaly type is determined to be liquid backflow.

[0147] In one embodiment, a computer program product is provided, including a computer program that, when executed by a processor, performs the following steps:

[0148] During the process of injecting the reaction liquid into the reaction chamber, optical data of the reaction liquid in the reaction chamber is acquired;

[0149] The process of injecting the reaction liquid into the reaction chamber is monitored to ensure it is proceeding normally, based on changes in optical data.

[0150] In one embodiment, when the computer program is executed by the processor, it also performs the following steps: determining a slope curve based on optical data; and monitoring whether the process of injecting the reaction liquid into the reaction chamber is normal based on the slope curve.

[0151] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: determining whether there is a target curve in the slope curve that matches the reference slope curve; if there is a target curve in the slope curve, determining that the process of injecting the reaction liquid into the reaction chamber is normal.

[0152] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: smoothing the optical data according to a preset data processing window to obtain smoothed optical data; the data processing window is used to limit the amount of optical data to be processed; the smoothed optical data is subjected to linear fitting according to the data processing window to obtain multiple fitted lines; the slope of each fitted line is calculated, and the slope curve is determined based on the slope.

[0153] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: if the target curve is not present in the slope curve, it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal.

[0154] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: if it is determined that the process of injecting the reaction liquid into the reaction chamber is abnormal, it determines the type of abnormality in the process of injecting the reaction liquid into the reaction chamber based on the slope curve.

[0155] In one embodiment, when the computer program is executed by the processor, it further performs the following steps: if there are points in the slope curve with a slope less than zero, then the anomaly type is determined to be liquid backflow.

[0156] Those skilled in the art will understand that all or part of the processes in the methods of the above embodiments can be implemented by a computer program instructing related hardware. The computer program can be stored in a non-volatile computer-readable storage medium. When executed, the computer program can include the processes of the embodiments of the above methods. Any references to memory, databases, or other media used in the embodiments provided in this application can include at least one of non-volatile and volatile memory. Non-volatile memory can include read-only memory (ROM), magnetic tape, floppy disk, flash memory, optical memory, high-density embedded non-volatile memory, resistive random access memory (ReRAM), magnetic random access memory (MRAM), ferroelectric random access memory (FRAM), phase change memory (PCM), graphene memory, etc. Volatile memory can include random access memory (RAM) or external cache memory, etc. By way of illustration and not limitation, RAM can take many forms, such as Static Random Access Memory (SRAM) or Dynamic Random Access Memory (DRAM). The databases involved in the embodiments provided in this application may include at least one type of relational database and non-relational database. Non-relational databases may include, but are not limited to, blockchain-based distributed databases. The processors involved in the embodiments provided in this application may be general-purpose processors, central processing units, graphics processing units, digital signal processors, programmable logic devices, quantum computing-based data processing logic devices, etc., and are not limited to these.

[0157] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0158] The embodiments described above are merely illustrative of several implementation methods of this application, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of this patent application. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this application, and these all fall within the protection scope of this application. Therefore, the protection scope of this application should be determined by the appended claims.

Claims

1. A method of monitoring liquid injection, characterized by, The monitoring method comprises: obtaining optical data of the reaction liquid in the reaction cavity during injection of the reaction liquid into the fluorescence quantitative PCR reaction cavity; the reaction liquid has an optical signal before amplification; monitoring whether the injection process of the reaction liquid into the reaction cavity is normal according to the change of the optical data; wherein, the monitoring whether the injection process of the reaction liquid into the reaction cavity is normal according to the change of the optical data comprises: determining a slope curve according to the optical data; monitoring whether the injection process of the reaction liquid into the reaction cavity is normal according to the slope curve; if it is determined that the injection process of the reaction liquid into the reaction cavity is abnormal, determining the abnormal type of the injection process of the reaction liquid into the reaction cavity according to the slope curve; the monitoring whether the injection process of the reaction liquid into the reaction cavity is normal according to the slope curve comprises: determining whether a target curve matching a reference slope curve exists in the slope curve; the reference slope curve is a slope curve corresponding to the optical data when the injection process of the reaction liquid into the reaction cavity is normal; if the target curve exists in the slope curve, it is determined that the injection process of the reaction liquid into the reaction cavity is normal.

2. The monitoring method according to claim 1, characterized in that, the determining the slope curve according to the change of the optical data comprises: performing smoothing processing on the optical data according to a preset data processing window to obtain smoothed optical data; the data processing window is used to limit the number of the optical data to be processed; performing linear fitting processing on the smoothed optical data according to the data processing window to obtain a plurality of fitting straight lines; calculating the slope of each fitting straight line, and determining the slope curve according to the slope.

3. The monitoring method of claim 1, wherein, the monitoring method further comprises: if the target curve does not exist in the slope curve, it is determined that the injection process of the reaction liquid into the reaction cavity is abnormal.

4. The monitoring method of claim 1, wherein, the determining the abnormal type of the injection process of the reaction liquid into the reaction cavity according to the slope curve comprises: if a point with a slope less than zero exists in the slope curve, it is determined that the abnormal type is liquid backflow.

5. A system for monitoring liquid infusion, characterized by The monitoring system comprises: a control device and a polymerase chain reaction detector; the polymerase chain reaction detector is used to detect optical data of the reaction liquid in the reaction cavity during injection of the reaction liquid into the fluorescence quantitative PCR reaction cavity, and send the optical data to the control device; the reaction liquid has an optical signal before amplification; the control device is used to monitor whether the injection process of the reaction liquid into the reaction cavity is normal according to the change of the optical data; the control device is specifically used to determine a slope curve according to the optical data; monitor whether the injection process of the reaction liquid into the reaction cavity is normal according to the slope curve; if it is determined that the injection process of the reaction liquid into the reaction cavity is abnormal, determine the abnormal type of the injection process of the reaction liquid into the reaction cavity according to the slope curve. The control device is further configured to determine whether the slope curve has a target curve matching a reference slope curve; the reference slope curve is a slope curve corresponding to optical data when the reaction liquid is normally injected into the reaction chamber; and if the target curve exists in the slope curve, it is determined that the reaction liquid is normally injected into the reaction chamber.

6. The monitoring system of claim 5, wherein, The polymerase chain reaction detector comprises an optical detection unit and a cartridge; the optical detection unit is provided with an optical channel; and the cartridge is provided with a reaction chamber. The control device is configured to control the optical detection unit to move to align the optical channel with the reaction chamber before the reaction liquid is injected into the reaction chamber; and control the optical detection unit to return to the original position after the reaction liquid is injected into the reaction chamber.

7. The monitoring system of claim 6, wherein The control device is further configured to control the optical detection unit to detect the optical data during the injection of the reaction liquid into the reaction chamber, and acquire the optical data from the optical detection unit.

8. A monitoring device for liquid injection, characterized in that The monitoring device comprises: The acquisition module is configured to acquire optical data of the reaction liquid in the reaction chamber during injection of the reaction liquid into the fluorescence quantitative PCR reaction chamber; the reaction liquid has an optical signal before amplification; The monitoring module is configured to monitor whether the injection of the reaction liquid into the reaction chamber is normal according to changes in the optical data; The monitoring module comprises a determination unit, a monitoring unit and an abnormal type determination module; the determination unit is configured to determine a slope curve according to the optical data; the monitoring unit is configured to monitor whether the injection of the reaction liquid into the reaction chamber is normal according to the slope curve; and the abnormal type determination module is configured to determine an abnormal type of the injection of the reaction liquid into the reaction chamber according to the slope curve if it is determined that the injection of the reaction liquid into the reaction chamber is abnormal. The monitoring unit is specifically configured to determine whether the slope curve has a target curve matching a reference slope curve; the reference slope curve is a slope curve corresponding to optical data when the reaction liquid is normally injected into the reaction chamber; and if the target curve exists in the slope curve, it is determined that the reaction liquid is normally injected into the reaction chamber. 9.A computer device, comprising a memory and a processor, wherein the memory stores a computer program, and the computer device is configured to perform the method according to any one of claims 1-8 when the computer program is executed by the processor. The processor executes the computer program to implement the steps of the method of any one of claims 1 to 4.

10. A computer-readable storage medium having stored thereon a computer program, characterized in that, The computer program is executed by the processor to implement the steps of the method of any one of claims 1 to 4.

11. A computer program product comprising a computer program, characterized in that, The computer program is executed by the processor to implement the steps of the method of any one of claims 1 to 4. The computer program is executed by the processor to implement the steps of the method of any one of claims 1 to 4.

Images