A full-spectrum flow cytometer and method of using the same

Abstract

The present invention discloses a full-spectrum flow cytometer and a method for using the same, comprising an instrument main case and a working case, wherein the instrument main case is installed at the left end of the working case, a detachable maintenance panel is arranged on the front outer wall of the instrument main case, an external joint is arranged on the front outer wall near the lower left corner of the instrument main case, a novel small-distance adjustment device is added to the bottom of the full-spectrum flow cytometer, and the novel small-distance adjustment device can be adjusted to a storage state through an adjustment mechanism when not in use, so that the normal placement and use of the entire full-spectrum flow cytometer through a plurality of placement foot cups will not be affected, and when the use position of the full-spectrum flow cytometer needs to be fine-tuned, the small-distance adjustment device can be adjusted to a displacement function state through two adjustment mechanisms, so that the full-spectrum flow cytometer can be displacement-adjusted on a desktop or the ground through the displacement function of the small-distance adjustment device.

Description

Technical Field

[0001] The present invention belongs to the technical field related to full-spectrum flow cytometers, and particularly relates to a full-spectrum flow cytometer and a method for using the same. Background Art

[0002] Full spectrum multicolor flow cytometer is an analytical instrument used in the fields of basic medicine and clinical medicine. It is mainly used for DNA ploidy analysis, cell viability experiments, counting reticulocytes in peripheral blood, CD34 positive stem cell counts in peripheral blood and bone marrow collections, cross-lymphocyte and granulocyte cytotoxicity experiments, platelet autoantibody detection, transplant cross-matching, detection of cell activation effects after antigen or cell mitosis stimulation, detection of intracellular factors, cell proliferation status detection, chromosome analysis, cell apoptosis analysis and other studies.

[0003] The existing full-spectrum flow cytometer technology has the following problems: the existing full-spectrum flow cytometer is large and heavy. During use, if the full-spectrum flow cytometer is placed in a poor position and angle, it will affect its normal use. When the placement position and angle of the full-spectrum flow cytometer need to be adjusted, the full-spectrum flow cytometer needs to be moved solely by manpower, which can easily cause damage to the full-spectrum flow cytometer during adjustment and increase physical labor, which is not conducive to the flexible use of the full-spectrum flow cytometer. Summary of the Invention

[0004] The object of the present invention is to provide a full-spectrum flow cytometer and a method for using the same, so as to solve the problem raised in the above-mentioned background art that when the placement and angle of the full-spectrum flow cytometer need to be adjusted, the full-spectrum flow cytometer needs to be moved solely by manpower, which can easily cause the full-spectrum flow cytometer to be damaged during adjustment and also increases physical labor.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a full-spectrum flow cytometer and a method for using the same, comprising an instrument main case and a working case, wherein the instrument main case is installed at the left end of the working case, a detachable maintenance panel is provided on the front outer wall of the instrument main case, an external joint is provided on the instrument main case near the lower left corner and on the front outer wall, the instrument main case and the working case are both provided with foot cups near the bottom of the front and rear sides, a small distance adjustment device is provided on the bottom of the instrument main case and the working case, a case door A and a case door B are embedded in the interior of the front outer wall near the middle section of the working case, the case door A is located on the left side of the case door B, the upper ends of the case door A and the case door B are connected with a lockable buckle, a transparent window is embedded in the middle section of the case door B, an automatic sample loader is vertically provided on the rear side of the case door B and located inside the working case, and the small distance adjustment device comprises an operating head, a support screw A, a displacement ball, a ball hole, a support plate, an angle A deflection connector, a rotating shaft connecting seat, an internal thread adjustment cylinder and a support screw B, the support plate is horizontally arranged on the outside of the lower end outer wall of the instrument main case and the working case, and an angle deflection connector is provided on the upper outer walls on both sides of the support plate, and the upper ends of the two angle deflection connectors are provided with a rotating shaft connecting seat, and an internal thread adjustment cylinder is vertically provided on the outer wall of the instrument main case near the lower left end and the outer wall of the working case near the lower right end, and the two internal thread adjustment cylinders are respectively penetrated by a support screw A and a support screw B vertically, and the support screw A and the support screw B are respectively located on the outside of the left end outer wall of the instrument main case and the outside of the right end outer wall of the working case, and the lower ends of the support screw A and the support screw B are respectively fixedly connected to the two rotating shaft connecting seats, and the upper ends of the support screw A and the support screw B are both provided with an operating head, and a plurality of ball holes with downward openings are equidistantly arranged on the interior of the support plate, and the interiors of the plurality of ball holes are downwardly penetrated by displacement balls.

[0006] Preferably, the lower outer walls of the instrument main case and the working case are kept flush, the upper front corners of the instrument main case and the working case are both curved, and the detachable maintenance panel is fixedly connected to the instrument main case by a plurality of embedded buckles.

[0007] Preferably, the left end of the box door A and the right end of the box door B are connected to the working chassis through embedded hinges, and the box door A and the box door B can be opened outward to an angle greater than ninety degrees through the embedded hinges. After the box door A and the box door B are closed, the front outer wall is flush with the front outer wall of the working chassis.

[0008] Preferably, the instrument main case and working case are fixedly connected to the bottom placement foot cups by screws, and the multiple placement foot cups can be individually adjusted to five centimeters in height, and anti-slip pads are provided on the lower end outer walls of the multiple placement foot cups.

[0009] Preferably, the length of the support plate is greater than the sum of the lengths of the instrument main case and the working case, and after the upper outer wall of the support plate is attached to the lower outer walls of the instrument main case and the working case, the lower outer wall of the support plate does not contact the ground or the desktop.

[0010] Preferably, the ball hole is a three-quarter volume spherical hole, and one-quarter of the lower end of the displacement ball passes through the lower end opening of the ball hole, and the lower ends of the multiple displacement balls are maintained on the same horizontal plane.

[0011] Preferably, the lengths and diameters of the support screws A and B are equal, the threads on the outer walls of the support screws A and B engage with the threads on the inner wall of the internal thread adjustment cylinder, and the cross-sectional shape of the operating head is a regular hexagon.

[0012] Preferably, the steps of the method of use are as follows:

[0013] Step 1: First, the full-spectrum flow cytometer needs to be moved to the location where it needs to be used. Then, the multiple foot cups at the bottom of the instrument main case and the working case need to be used to place the full-spectrum flow cytometer on the ground or table. Then, the height of the multiple foot cups can be adjusted according to the use requirements. By changing the height of the foot cups, the use height of the entire full-spectrum flow cytometer can be changed, so that the full-spectrum flow cytometer can be used at the optimal height.

[0014] Step 2: Then it is necessary to connect the power supply to the instrument main case and the working case of the full-spectrum flow cytometer, and at the same time, it is necessary to connect the full-spectrum flow cytometer to the external control terminal through multiple external connectors on the instrument main case, so that the full-spectrum flow cytometer can transmit the detection structure to the terminal in real time, and then the box door A and the box door B can be opened, and the sample to be tested can be placed inside the working case, and then the box door A and the box door B can be closed again, and then the full-spectrum flow cytometer can be started normally;

[0015] Step 3: The cells to be tested, stained with a specific fluorescent dye, are placed in a sample tube. Under the pressure of gas, the cells enter a flow chamber filled with sheath fluid. Under the constraint of the sheath fluid, the cells are arranged in a single file and ejected from the nozzle of the flow chamber to form a cell column. The scattered light signal and fluorescence signal are excited by the laser. The signals are collected by respective laser detectors, amplified by photomultiplier tubes (PMTs), converted into electrical signals, and analyzed by a computer system.

[0016] Compared with the prior art, the present invention provides a full-spectrum flow cytometer and a method of using the same, which have the following beneficial effects:

[0017] 1. The present invention adds a novel small distance adjustment device to the bottom of the full-spectrum flow cytometer. When not in use, the novel small distance adjustment device can be adjusted to a storage state through the adjustment mechanism, which will not affect the normal placement and use of the entire full-spectrum flow cytometer through multiple placement foot cups. When the use position of the full-spectrum flow cytometer needs to be fine-tuned, the small distance adjustment device can be adjusted to a displacement function state through two adjustment mechanisms. In this way, the full-spectrum flow cytometer can be displaced and adjusted on a desktop or the ground through the displacement function of the small distance adjustment device. Especially when the full-spectrum flow cytometer is placed for the first time, the placement position of the full-spectrum flow cytometer can be adjusted by the small distance adjustment device so that it can be used in the optimal placement state, thereby greatly increasing the practicality and flexibility of use of the full-spectrum flow cytometer, avoiding the situation where the instrument is easily damaged by simply carrying it manually when adjusting the placement angle of the full-spectrum flow cytometer, and thus avoiding unnecessary economic losses.

[0018] 2. When the small distance adjustment device of the present invention is in the storage state, the upper outer wall of the support plate is in contact with the bottom outer wall of the instrument main case and the working case of the full-spectrum flow cytometer, and the lower outer wall of the support plate is not in contact with the placement ground or desktop, thereby ensuring that the full-spectrum flow cytometer can be placed and used normally through multiple placement foot cups. When it is necessary to adjust the displacement of the placed full-spectrum flow cytometer, the displacement function of the small distance adjustment device is needed to assist the full-spectrum flow cytometer in displacement adjustment. During the specific operation, first, you need to grab the operating head at the upper end of the support screw A and turn it clockwise. When the support screw A is turned clockwise, it will slowly descend through the threaded pushing action of the internal threaded adjustment cylinder, so that the support screw will synchronously drive the right end of the support plate to move downward when it descends, until the bottom of the right end of the support plate is in contact with the ground or table. Then, the support screw B is driven to rotate through the operating head in the same way, and when the support screw B descends, it will push the left end of the support plate to move downward until the bottom of the left end of the support plate is also in contact with the ground or table. Because there are multiple ball bearing holes with downward openings inside the support plate, and displacement balls are penetrated downward inside the multiple ball bearing holes, when the support plate is adjusted to the left When the bottoms of both right ends are in contact with the ground or desktop, multiple displacement balls passing through the ball bearing holes are in contact with the ground or desktop, and multiple displacement balls will replace the foot cups to place the full-spectrum flow cytometer. Therefore, the full-spectrum flow cytometer can be directly pushed by the rolling action of the displacement balls. In this way, the placed full-spectrum flow cytometer can be adjusted to a small distance, so that the full-spectrum flow cytometer can be adjusted to the optimal position and angle for use. After adjustment, the support screws A and B need to be rotated counterclockwise to move the support plate upward, which will make the multiple placements The foot cup plays a placement role again to place the full-spectrum flow cytometer. During the above adjustment process, the shaft connecting seat not only connects the support screw A and the angle deflection connector and the support screw B and the angle deflection connector, but also allows the support screw A and the support screw B to rotate independently, which will not cause the support plate to rotate. The two angle deflection connectors, while playing a connecting role, can ensure that the left and right ends of the support plate can rotate downward independently when the support screw A and the support screw B move downward, which will not hinder the adjustment operation of the support plate, thereby increasing the flexibility of use of the small-distance adjustment device. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0020] Figure 1A schematic diagram of the three-dimensional structure of a full-spectrum flow cytometer and its use method proposed in the present invention;

[0021] Figure 2 This is a schematic diagram of the planar structure of a full-spectrum flow cytometer and its use method proposed in the present invention;

[0022] Figure 3 This is a schematic diagram of the structure of the small distance adjustment device proposed by the present invention in the retracted state;

[0023] Figure 4 This is a schematic diagram of the structure of the small distance adjustment device proposed by the present invention in use;

[0024] In the figure: 1. Instrument main case; 2. External connector; 3. Removable maintenance panel; 4. Foot cup placement; 5. Working case; 6. Case door A; 7. Case door B; 8. Transparent window; 9. Small distance adjustment device; 10. Automatic sample loader; 11. Locking handle; 12. Operating head; 13. Support screw A; 14. Displacement ball; 15. Ball hole; 16. Support plate; 17. Angle deflection connector; 18. Shaft connector; 19. Internal thread adjustment cylinder; 20. Support screw B. DETAILED DESCRIPTION

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] Example 1

[0027] See also Figure 1 、 Figure 2The present invention provides a technical solution: a full-spectrum flow cytometer and a method for using the same, comprising an instrument main case 1 and a working case 5, wherein the instrument main case 1 is mounted on the left end of the working case 5, and a detachable maintenance panel 3 is provided on the front outer wall of the instrument main case 1. The lower outer walls of the instrument main case 1 and the working case 5 are kept flush, and the upper front corners of the instrument main case 1 and the working case 5 are both treated with curved surfaces, and the detachable maintenance panel 3 is fixedly connected to the instrument main case 1 through a plurality of embedded buckles. When the internal components of the instrument main case 1 are damaged, the detachable maintenance panel 3 can be directly opened for detachable maintenance. The panel 3 can quickly repair or replace the components inside the instrument main box 1, which can reduce the maintenance difficulty of the full-spectrum flow cytometer. The instrument main box 1 is provided with an external connector 2 near the lower left corner and on the front outer wall. The instrument main box 1 and the working box 5 are provided with foot cups 4 near the bottom of the front and rear sides. The instrument main box 1 and the working box 5 are fixedly connected to the foot cups 4 at the bottom by screws. The multiple foot cups 4 can be individually adjusted to a height of five centimeters. The lower outer walls of the multiple foot cups 4 are provided with anti-slip pads. Because the full-spectrum flow cytometer is The instrument is placed and used by multiple placement foot cups 4, and the placement foot cups 4 are installed with anti-skid pads at the bottom, and the anti-skid pads can fully contact with the placement surface and increase the friction of the contact surface, so that the full-spectrum flow cytometer can be placed and used more stably and securely. A small distance adjustment device 9 is provided at the bottom of the instrument main case 1 and the working case 5. The working case 5 is embedded with a box door A6 and a box door B7 inside the outer wall near the front end of the middle section. The box door A6 is located on the left side of the box door B7. The upper ends of the box doors A6 and B7 are connected with a lockable buckle 11. The middle section of the box door B7 is embedded with a transparent sight glass. Window 8, an automatic sample loader 10 is vertically arranged on the rear side of the box door B7 and located inside the working chassis 5. The left end of the box door A6 and the right end of the box door B7 are connected to the working chassis 5 through embedded hinges. The box door A6 and the box door B7 can be opened outward at an angle greater than ninety degrees through the embedded hinges. After the box door A6 and the box door B7 are closed, the front outer wall is flush with the front outer wall of the working chassis 5. The box door A6 and the box door B7 can be rotated and opened at a large angle, which makes it more convenient to place the cell sample inside the working chassis 5, thereby avoiding damage to the sample caused by mistakes when placing the sample.

[0028] Example 2

[0029] See also Figure 1 、 Figure 2 、 Figure 3 、 Figure 4The present invention provides a technical solution: a full-spectrum flow cytometer and a method for using the same. The small-distance adjustment device 9 includes an operating head 12, a support screw A13, a displacement ball 14, a ball hole 15, a support plate 16, an angle deflection connector 17, a rotating shaft connection seat 18, an internal thread adjustment cylinder 19 and a support screw B20. The support plate 16 is laterally arranged on the outside of the lower outer wall of the instrument main case 1 and the working case 5. The length of the support plate 16 is greater than the sum of the lengths of the instrument main case 1 and the working case 5. After the upper outer wall of the support plate 16 is attached to the lower outer wall of the instrument main case 1 and the working case 5, the lower outer wall of the support plate 16 does not contact the ground or the desktop. Angle deflection connectors 17 are provided on the upper outer walls on both sides of the support plate 16. The upper ends of the two angle deflection connectors 17 are each provided with a rotating shaft connection seat 18, and an internal thread adjustment cylinder 19 is vertically provided on the outer wall of the instrument main case 1 near the lower left end and on the outer wall of the working case 5 near the lower right end. The two internal thread adjustment cylinders 19 are respectively penetrated by a support screw A13 and a support screw B20 vertically. The support screw A13 and the support screw B20 are respectively located on the outside of the outer wall of the left end of the instrument main case 1 and the outside of the outer wall of the right end of the working case 5. The lower ends of the support screw A13 and the support screw B20 are respectively fixedly connected to the two rotating shaft connection seats 18, and the upper ends of the support screw A13 and the support screw B20 are both provided with an operating head 12. The length and diameter of the support screw A13 and the support screw B20 are equal. The threads on the outer wall of the support screw B20 are meshed with the threads on the inner wall of the internal thread adjustment cylinder 19. The cross-sectional shape of the operating head 12 is a regular hexagon. The interior of the support plate 16 is equidistantly provided with a plurality of ball holes 15 with downward openings. The interiors of the plurality of ball holes 15 are penetrated downward by displacement balls 14. The ball holes 15 are three-quarter volume spherical holes. A quarter of the lower end of the displacement ball 14 penetrates the lower end opening of the ball hole 15. The lower ends of the plurality of displacement balls 14 are kept on the same horizontal plane. When operating the small distance adjustment device 9, you first need to grab the operating head 12 at the upper end of the support screw A13 and turn it clockwise. When the support screw A13 rotates clockwise, it will slowly descend through the thread pushing action of the internal thread adjustment cylinder 19. This In this way, when the support screw rod descends, the right end of the support plate 16 will be synchronously driven to move downward until the bottom of the right end of the support plate 16 is in contact with the ground or table. Then, in the same way, the support screw rod B20 is driven to rotate by the operating head 12. When the support screw rod B20 descends, it will push the left end of the support plate 16 to move downward until the bottom of the left end of the support plate 16 is also in contact with the ground or table. Because the support plate 16 has a plurality of ball holes 15 opening downward, and the plurality of ball holes 15 have displacement balls 14 passing through them downward, when the left and right bottom ends of the support plate 16 are in contact with the ground or table after adjustment, the plurality of displacement balls 14 passing through the ball holes 15 are all in contact with the ground or table.The multiple displacement balls 14 replace the foot cups 4 to place the full-spectrum flow cytometer. Therefore, the full-spectrum flow cytometer can be directly pushed by the rolling action of the displacement balls 14. In this way, the placement position of the full-spectrum flow cytometer can be adjusted by a small distance, so that the full-spectrum flow cytometer can be adjusted to the optimal position and angle for use.

[0030] The working principle and use process of the present invention: After the present invention is installed, when using the full-spectrum flow cytometer, first, the full-spectrum flow cytometer needs to be moved to the position where it needs to be used, and then the multiple placement feet 4 at the bottom of the instrument main case 1 and the working case 5 need to be placed on the ground or table where the full-spectrum flow cytometer needs to be used, and then the use height of the multiple placement feet 4 can be adjusted according to the use requirements. By changing the height of the placement feet 4, the use height of the entire full-spectrum flow cytometer can be changed, so that the full-spectrum flow cytometer can be used at the optimal height, and then the instrument main case 1 and the working case 5 of the full-spectrum flow cytometer need to be powered on, and at the same time, the full-spectrum flow cytometer needs to be placed on the ground or table through the multiple external connectors 2 on the instrument main case 1. The cytometer is connected to the external control terminal so that the full-spectrum flow cytometer can transmit the detection structure to the terminal in real time. Then the box door A6 and the box door B7 can be opened, and the sample to be tested is placed inside the working chassis 5. Then the box door A6 and the box door B7 are closed again, and then the full-spectrum flow cytometer can be started normally. During the detection, the cells to be tested after being stained with specific fluorescent dyes are placed in the sample tube. The cells enter the flow chamber filled with sheath fluid under the pressure of the gas. Under the constraint of the sheath fluid, the cells are arranged in a single row and ejected from the nozzle of the flow chamber to form a cell column. The scattered light signal and the fluorescence signal are excited by the laser, and the signals are collected by their respective laser detectors. The signals are then amplified by the photomultiplier tube (PMT), converted into electrical signals, and analyzed by the computer system.

[0031] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A full-spectrum flow cytometer, comprising an instrument main chassis (1) and a working chassis (5), characterized in that: The instrument main case (1) is installed at the left end of the working case (5), a detachable maintenance panel (3) is provided on the front outer wall of the instrument main case (1), an external joint (2) is provided on the front outer wall near the lower left corner of the instrument main case (1), foot cups (4) are provided near the bottom of the front and rear sides of the instrument main case (1) and the working case (5), a small distance adjustment device (9) is provided at the bottom of the instrument main case (1) and the working case (5), a case door A (6) and a case door B (7) are embedded in the inner part of the front outer wall near the middle section of the working case (5), and the case door A (6) is located on the left side of the box door B (7), the box door A (6) and the upper end of the box door B (7) are connected with a lockable buckle (11), the middle section of the box door B (7) is embedded with a transparent window (8), the rear side of the box door B (7) and the inside of the working box (5) are provided with an automatic sample loader (10), the small distance adjustment device (9) includes an operating head (12), a support screw A (13), a displacement ball (14), a ball hole (15), a support plate (16), an angle deflection connector (17), a shaft connection seat (18), an internal thread adjustment cylinder (19) and a support screw Rod B (20), the support plate (16) is transversely arranged on the outside of the lower end outer wall of the instrument main case (1) and the working case (5), and angle deflection connectors (17) are arranged on the upper end outer walls of the left and right sides of the support plate (16), and the upper ends of the two angle deflection connectors (17) are arranged with a rotating shaft connection seat (18), and the inner thread adjustment cylinder (19) is vertically arranged on the outer wall of the instrument main case (1) near the lower left end and the outer wall of the working case (5) near the lower right end, and the two inner thread adjustment cylinders (19) are vertically penetrated by the support screw A (13) and the support screw A (13) respectively. Rod B (20), the support screw A (13) and the support screw B (20) are respectively located outside the outer wall of the left end of the instrument main case (1) and outside the outer wall of the right end of the working case (5), the lower ends of the support screw A (13) and the support screw B (20) are respectively fixedly connected to two rotating shaft connecting seats (18), the upper ends of the support screw A (13) and the support screw B (20) are both provided with an operating head (12), and the interior of the support plate (16) is equidistantly provided with a plurality of ball holes (15) opening downward, and the interiors of the plurality of ball holes (15) are all penetrated downward by displacement balls (14).

2. A full spectrum flow cytometer according to claim 1, characterized in that: The lower outer walls of the instrument main case (1) and the working case (5) are kept flush, the upper front edges and corners of the instrument main case (1) and the working case (5) are both treated with curved surfaces, and the detachable maintenance panel (3) is fixedly connected to the instrument main case (1) via a plurality of embedded buckles.

3. A full spectrum flow cytometer according to claim 1, characterized in that: The left end of the box door A (6) and the right end of the box door B (7) are connected to the working case (5) via embedded hinges. Both the box door A (6) and the box door B (7) can be opened outwards to an angle greater than ninety degrees via the embedded hinges. When the box door A (6) and the box door B (7) are closed, the front outer wall is flush with the front outer wall of the working case (5).

4. A full spectrum flow cytometer according to claim 1, characterized in that: The instrument main case (1) and the working case (5) are fixedly connected to the bottom foot cups (4) by screws, and the plurality of foot cups (4) can be individually adjusted in height by five centimeters. Anti-slip pads are arranged on the outer walls of the lower ends of the plurality of foot cups (4).

5. A full spectrum flow cytometer according to claim 1, characterized in that: The length of the support plate (16) is greater than the sum of the lengths of the instrument main case (1) and the working case (5); after the upper outer wall of the support plate (16) is attached to the lower outer walls of the instrument main case (1) and the working case (5), the lower outer wall of the support plate (16) does not contact the ground or the tabletop.

6. A full spectrum flow cytometer according to claim 1, characterized in that: The ball hole (15) is a three-quarter volume spherical hole, and one-quarter of the lower end of the displacement ball (14) passes through the lower end opening of the ball hole (15), and the lowermost ends of the plurality of displacement balls (14) are maintained on the same horizontal plane.

7. A full spectrum flow cytometer according to claim 1, characterized in that: The lengths and diameters of the support screw A (13) and the support screw B (20) are equal, the threads on the outer walls of the support screw A (13) and the support screw B (20) are meshed with the threads on the inner wall of the internal thread adjustment cylinder (19), and the cross-sectional shape of the operating head (12) is a regular hexagon.

8. A method for using a full-spectrum flow cytometer as claimed in any one of claims 1 to 7, characterized in that: The steps of using the method are as follows: Step 1: First, the full-spectrum flow cytometer needs to be moved to the location where it is needed to be used, and then the full-spectrum flow cytometer needs to be placed on the ground or desktop where it is needed to be used through the multiple placement cups (4) at the bottom of the instrument main case (1) and the working case (5), and then the use height of the multiple placement cups (4) is adjusted according to the use requirements. By changing the height of the placement cups (4), the use height of the entire full-spectrum flow cytometer can be changed, so that the full-spectrum flow cytometer can be used at the optimal height; Step 2: Then, the instrument main case (1) and the working case (5) of the full-spectrum flow cytometer need to be powered on, and at the same time, the full-spectrum flow cytometer needs to be connected to an external control terminal through multiple external connectors (2) on the instrument main case (1), so that the full-spectrum flow cytometer can transmit the detection structure to the terminal in real time, and then the box door A (6) and the box door B (7) can be opened, and the sample to be detected can be placed in the working case (5), and then the box door A (6) and the box door B (7) can be closed again, and then the full-spectrum flow cytometer can be started normally; Step 3: Place the cells to be tested that have been stained with specific fluorescent dyes into a sample tube. The cells enter a flow chamber filled with sheath fluid under the pressure of gas. Under the constraint of the sheath fluid, the cells are arranged in a single row and ejected from the nozzle of the flow chamber to form a cell column. The scattered light signal and the fluorescence signal are excited by the laser, and the signals are collected by their respective laser detectors. The signals are then amplified by a photomultiplier tube (PMT), converted into electrical signals, and analyzed by a computer system.

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