Sample analysis device and method

Abstract

A sample analysis device and method provide a first type of measurement position and a second type of measurement position; transport a first container to the first type of measurement position and / or the second type of measurement position; irradiate the first type of measurement position with light of a first intensity, and / or irradiate the second type of measurement position with light of a second intensity; the first intensity is less than the second intensity; obtain optical detection information corresponding to the first intensity of light and / or the second intensity of light; and analyze the detection results of the sample based on the optical detection information corresponding to the first intensity of light and / or the second intensity of light.

Description

Technical Field

[0001] The present invention relates to a sample analysis device and method. Background Art

[0002] Sample analyzers are devices used to analyze the biochemical properties of samples. They are widely used in clinical medicine to help medical professionals diagnose patients' conditions. For example, a coagulometer can measure blood clotting time and the concentration or activity of related substances. Coagulometers can use optical methods to detect coagulation. Specifically, they illuminate the solution in the reaction cup during the reaction and analyze the scattered or transmitted light to obtain optical information such as the solution's absorbance, thereby determining the clotting time or the concentration of the analyte.

[0003] The optical method detects coagulation items through the optical information of the reaction solution's scattering, reflection or transmission of light. Therefore, when the sample contains interfering substances that change the reaction solution's scattering, reflection or transmission properties of light, this will affect the measurement, making the test results inaccurate. In severe cases, no test results can be obtained at all. SUMMARY OF THE INVENTION

[0005] Technical issues

[0006] The present invention mainly provides a sample analysis device and method.

[0007] Solution to the problem

[0008] Technical Solutions

[0009] According to the first aspect, an embodiment provides a sample analysis device, comprising:

[0010] An optical detection component includes a first type of measurement position and a first detector corresponding to the first type of measurement position, a second type of measurement position and a second detector corresponding to the second type of measurement position;

[0011] an illumination component configured to provide light of a first intensity to illuminate a first container located at a first type of measurement position and containing a measurement sample, and to provide light of a second intensity to illuminate the first container located at a second type of measurement position and containing a measurement sample; the measurement sample being prepared from a sample and a reagent; the first intensity being less than the second intensity;

[0012] a transporting component, configured to transport the first container to the first type of measurement location or the second type of measurement location;

[0013] The control component is used to control the transport component to transport the first container containing the measurement sample to the first type measurement position or the second type measurement position for optical detection based on the interference detection information of the sample.

[0014] In one embodiment, the optical detection component is configured to receive an output light signal after the first container at the first type measurement position or the second type measurement position is illuminated by the illumination component, and convert the output light signal into a corresponding electrical signal; the output light signal includes at least one of transmitted light, reflected light, or scattered light;

[0015] The sample analysis device further includes an analysis component for analyzing the electrical signal to perform analysis on the sample detection items.

[0016] In one embodiment, the sample analysis device further includes an interference detection component for performing interference detection on the sample to obtain the interference detection information, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value, and the control component is used to control the transport component to transport the first container to the first type of measurement position when the interference content of the sample does not exceed the threshold value; or to control the transport component to transport the first container to the second type of measurement position when the interference content of the sample exceeds the threshold value.

[0017] In one embodiment, the interference detection component includes an interference detection position and a third detector located on one side of the interference detection position; the illumination component is used to illuminate a second container located at the interference detection position and containing at least a sample, and the interference detection component is used to receive an output light signal of the second container after being illuminated by the illumination component to obtain interference detection information of the sample.

[0018] In one embodiment, the sample analysis device further includes a dispensing component, and the control component is used to control the dispensing component to dispense a portion of the sample and the diluent into the second container, and to control the dispensing component to dispense another portion of the sample and the detection reagent into the first container to prepare the measurement sample.

[0019] In one embodiment, the first container is a cuvette, and the second container is a colorimetric cell.

[0020] In one embodiment, the illumination component includes a first light source and a multi-fiber optical fiber bundle, which respectively provide light of the first intensity to the first type measurement position and the interference object detection position.

[0021] In one embodiment, the illumination component further includes a second light source for providing light of the second intensity to illuminate the first container located at the second type of measurement position and containing the measurement sample.

[0022] In one embodiment, the first light source is a multi-wavelength light source for providing light of at least a first wavelength, a second wavelength and a third wavelength; preferably, the first wavelength range is 340nm-420nm, the second wavelength range is 520nm-590nm, and the third wavelength range is 660nm-800nm.

[0023] In one embodiment, the second light source is a single-wavelength light source for providing light of a fourth wavelength, where the fourth wavelength is not less than any one of the first wavelength, the second wavelength, or the third wavelength.

[0024] In one embodiment, the illumination component illuminates a first container containing a measurement sample at the first type of measurement position with light of a first intensity, and the first detector is used to receive an output light signal of the first container after being illuminated by the illumination component to obtain interference detection information of the sample.

[0025] In one embodiment, the sample is a blood sample, and the interfering substance includes at least one of hemoglobin, bilirubin, and chyle.

[0026] In one embodiment, the interference detection information includes at least one of the absorbance of light of different wavelengths or the transmittance of light of the sample.

[0027] According to a second aspect, an embodiment provides a sample analysis device, comprising:

[0028] An optical detection component includes a first type of measurement position and a first detector corresponding to the first type of measurement position, a second type of measurement position and a second detector corresponding to the second type of measurement position;

[0029] a transport component for transporting a container containing a measurement sample to at least one of the first type measurement site or the second type measurement site for optical detection; the measurement sample is prepared from a sample and a reagent;

[0030] an illumination component configured to provide light of a first intensity to illuminate a first container located at a first type of measurement position and containing a measurement sample, and to provide light of a second intensity to illuminate a first container located at a second type of measurement position and containing a measurement sample; the first intensity being less than the second intensity; the first detector being configured to obtain first optical detection information corresponding to the light of the first intensity, and the second detector being configured to obtain second optical detection information corresponding to the light of the second intensity;

[0031] An analyzing component is used to analyze the detection result of the sample according to at least one of the first optical detection information or the second optical detection information.

[0032] In one embodiment, the sample analysis device further includes a sample interference detection component and a control component, wherein the sample interference detection component is used to perform interference detection on the sample to obtain interference detection information of the sample, and the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value, and the control component is used to control the transport component to transport the first container to the first type of measurement position when the interference content of the sample does not exceed the threshold value; or to control the transport component to transport the first container to the second type of measurement position when the interference content of the sample exceeds the threshold value.

[0033] In one embodiment, the illumination component illuminates a first container containing a measurement sample at the first type measurement position with light of a first intensity, and the first detector is further used to receive an output light signal of the first container after being illuminated by the illumination component to obtain a luminous flux of the measurement sample; the transport component is used to transport the first container to the second type measurement position when the luminous flux of the measurement sample does not exceed a threshold value.

[0034] According to a third aspect, an embodiment provides a sample analysis method, comprising:

[0035] Providing a first type of measurement bit and a second type of measurement bit;

[0036] Dispensing the sample and reagent into a first container to prepare a test specimen;

[0037] transporting the first container to a first type of measurement location and / or a second type of measurement location;

[0038] irradiating a first type of measurement position with light of a first intensity, and / or irradiating a second type of measurement position with light of a second intensity; the first intensity is less than the second intensity;

[0039] Acquiring optical detection information corresponding to the light of the first intensity and / or the light of the second intensity;

[0040] The detection result of the sample is analyzed according to the optical detection information corresponding to the light of the first intensity and / or the light of the second intensity.

[0041] In one embodiment, the sample analysis method further includes, before transporting the first container to the first type measurement location and / or the second type measurement location, performing an interference detection on the sample to obtain interference detection information of the sample, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value; transporting the first container to the first type measurement location and / or the second type measurement location includes:

[0042] If the interferent content of the sample does not exceed the threshold, transporting the first container to the first type of measurement site; or

[0043] If the interferent content of the sample exceeds a threshold, the first container is transported to the second type of measurement site.

[0044] In one embodiment, performing interference detection on the sample to obtain interference detection information of the sample includes:

[0045] Dispensing a portion of the sample and the diluent into a second container;

[0046] irradiating the second container with light of a first intensity;

[0047] receiving an output light signal after irradiating the second container;

[0048] converting the optical signal into an electrical signal;

[0049] The electrical signal is analyzed to obtain interference detection information of the sample.

[0050] In one embodiment, the sample analysis method further includes transporting the first container to the first type of measurement location and obtaining interfering substance detection information of the measurement sample measured at the first type of measurement location, wherein the interfering substance detection information indicates whether the interfering substance content of the sample exceeds a threshold value. Transporting the first container to the first type of measurement location and / or the second type of measurement location includes:

[0051] If the interferent content of the sample exceeds a threshold, the first container is transported from the first type of measurement site to the second type of measurement site.

[0052] In one embodiment, transporting the first container to the first type measurement location and / or the second type measurement location includes:

[0053] transporting the first container to the first type measurement station for optical measurement; and

[0054] The first container is transported to the second type measurement station for optical measurement.

[0055] In one embodiment, analyzing the detection results of the sample based on the optical detection information measured at the first type of measurement position and / or the second type of measurement position includes: selecting the optical detection information measured at the first type of measurement position or the second type of measurement position based on the interference detection information of the sample; and analyzing the detection results of the sample based on the selected optical detection information.

[0056] In one embodiment, the sample analysis method further includes obtaining interferor detection information of the sample, the interferor detection information being used to indicate whether an interferor content of the sample exceeds a threshold value; and selecting the optical detection information measured at the first type of measurement position or the second type of measurement position based on the interferor detection information of the sample includes:

[0057] If the interfering substance content of the sample does not exceed the threshold, selecting the optical detection information measured by the first type of measurement position;

[0058] If the interferent content of the sample exceeds a threshold, the optical detection information measured by the second type of measurement position is selected.

[0059] Advantageous Effects of the Invention

[0060] Brief description of the accompanying drawings BRIEF DESCRIPTION OF THE DRAWINGS

[0061] Figure 1 Schematic diagram of the absorption spectra of three interfering substances, hemoglobin, bilirubin and chyle, to light in different wavelength ranges;

[0062] Figure 2 Schematic diagram of optical curves of transmission responses of a normal sample and a severe chyle sample according to an embodiment;

[0063] Figure 3 Schematic diagram of optical curves of transmission response of a severe chyle sample when measured at an anti-interference measurement position and a conventional measurement position according to an embodiment;

[0064] Figure 4 is a schematic structural diagram of a sample analysis device according to an embodiment;

[0065] Figure 5 is a schematic structural diagram of a sample analysis device according to another embodiment;

[0066] Figure 6 is a schematic structural diagram of a sample analysis device according to another embodiment;

[0067] Figure 7 Schematic diagram of the structure of an illumination component according to an embodiment;

[0068] Figure 8 is a structural schematic diagram of a lighting component in another embodiment;

[0069] Figure 9 This is a schematic structural diagram of a sample analysis device according to another embodiment;

[0070] Figure 10 is a schematic structural diagram of a sample analysis device according to another embodiment;

[0071] Figure 11is a structural schematic diagram of a sample analysis device according to yet another embodiment;

[0072] Figure 12 A schematic flow chart of a sample analysis method according to an embodiment;

[0073] Figure 13 A schematic flow chart of a sample analysis method according to another embodiment;

[0074] Figure 14 A schematic diagram of a process of performing interference detection on the sample to obtain interference detection information of the sample according to an embodiment;

[0075] Figure 15 A schematic flow chart of a sample analysis method according to another embodiment;

[0076] Figure 16 FIG. 4 is a flow chart of a sample analysis method according to another embodiment.

[0077] Invention Embodiments

[0078] Modes for Carrying Out the Invention

[0079] The present invention will be further described in detail below by means of specific embodiments in conjunction with the accompanying drawings. Similar elements in different embodiments are numbered with associated similar elements. In the following embodiments, many detailed descriptions are provided to enable the present application to be better understood. However, those skilled in the art will readily appreciate that some of the features may be omitted in different circumstances, or may be replaced by other elements, materials, or methods. In some cases, some operations related to the present application are not shown or described in the specification. This is to avoid the core portion of the present application being overwhelmed by excessive descriptions, and for those skilled in the art, it is not necessary to describe these related operations in detail. They will fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0080] In addition, the features, operations, or characteristics described in the specification may be combined in any appropriate manner to form various embodiments. Furthermore, the steps or actions in the method description may be reordered or adjusted in a manner readily apparent to those skilled in the art. Therefore, the various sequences in the specification and drawings are provided solely for the purpose of clearly describing a particular embodiment and are not intended to be mandatory, unless otherwise specified.

[0081] The serial numbers assigned to components herein, such as "first," "second," etc., are used solely to distinguish the objects being described and do not convey any sequential or technical meaning. References to "connection" and "coupling" herein, unless otherwise specified, include both direct and indirect connections (couplings).

[0082] In the optical determination of coagulation, it can generally be divided into three methods: coagulation method, immunoturbidimetry and chromogenic substrate method. The chromogenic substrate method usually uses violet light or ultraviolet light of 340nm-420nm, and the chromogenic substrate method is usually used to determine test items such as antithrombin-III (AT-III or AT3). The immunoturbidimetry method usually uses yellow-green light of 520nm-590nm, and the immunoturbidimetry method is usually used to determine test items such as D-dimer (DD), fibrin / fibrinogen degradation products (FDP). The coagulation method usually uses red light or infrared light of 660nm-800nm, and the coagulation method is usually used to determine test items such as prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB).

[0083] Whether it is the coagulation method, immunoturbidimetry or chromogenic substrate method, in the optical determination of coagulation, when there are interfering substances in the sample, it will interfere with the detection. Normal plasma samples are generally light yellow and almost transparent, but some patients have symptoms such as jaundice, hemolysis or lipemia due to diseases and other reasons, which causes their plasma to be brownish yellow, slightly reddish or milky white. The symptom of jaundice indicates that there is an interfering substance such as bilirubin in the sample, the symptom of hemolysis indicates that there is an interfering substance such as hemoglobin in the sample, and the symptom of lipemia indicates that there is an interfering substance such as chyle in the sample. The absorption spectra of the three interfering substances are different. When there are interfering substances such as hemoglobin, bilirubin and chyle in the sample plasma, these interfering substances have strong absorption of light, so they will interfere with the sample detection and cause deviations in the test results. Hemoglobin, bilirubin and chyle can generally be collectively referred to as HIL interference, where H refers to hemoglobin, I refers to bilirubin, and L refers to chyle. Bilirubin, hemoglobin and chyle, the three interfering substances, appear in different colors, and their absorption spectra are as follows: Figure 1 As shown, bilirubin and hemoglobin have distinct absorption peaks—bilirubin has a strong absorption peak around 450nm, and hemoglobin has a strong absorption peak near 420nm. Both have almost no absorption at wavelengths above 660nm. Chyle, on the other hand, absorbs across the entire visible spectrum, decreasing with increasing wavelengths. However, even at 800nm, there is still some absorbance. Therefore, as can be seen from the figure, these three interfering substances have strong absorption of light in small wavelength bands—especially light below 600nm. This significantly reduces the transmittance of the mixture of sample and detection reagent, minimizing the amount of light actually received, affecting the accuracy and reliability of optical measurements. Sometimes, the amount of light received is even nearly zero, making it impossible to identify the reaction process between the sample and the detection reagent.

[0084] One solution to the problem of interference is to provide light in a wavelength band that will not be absorbed by the interference in the sample for detection, such as providing light with a longer wavelength, such as 800nm, to illuminate the mixture of the sample and the detection reagent. It can be clearly seen from the figure that hemoglobin and bilirubin have almost no absorption of light with a wavelength greater than 800nm, and chyle also has relatively little absorption of light with a wavelength greater than 800nm. This solution can effectively eliminate the influence of bilirubin and hemoglobin on sample determination, but it will still have an impact on chyle samples, especially re-tested chyle samples. Because although chyle absorbs relatively little light with a wavelength greater than 800nm, relatively speaking, for situations where more precise test results are required, these absorptions of light by chyle are still not negligible. In addition, when the concentration of chyle in the sample is relatively high, even in situations where the accuracy of the test results is generally required (such as physical examinations), these absorptions of light by chyle are still not negligible, and chyle will still seriously affect the accuracy of the test results. The transmission reaction optical curves of normal samples and severe chyle samples are shown in Figure 2. Figure 2 As shown, Figure 2 The horizontal axis is time in seconds, and the vertical axis is the light flux received by the transmission photodetector. Figure 2 It can be seen that for samples with severe chyle, due to its low transmittance, the light flux is almost zero from the beginning to the end of the reaction, and basically no light is transmitted - that is, the absorbance of the analyte is too large, exceeding the maximum absorbance test range of the detection optical sensor.

[0085] In addition, the above-mentioned scheme of switching to a larger wavelength for measurement is not applicable to items such as those detected by the chromogenic substrate method. This is because, from the detection principle, the chromogenic substrate method is due to the reaction between the sample and the detection reagent, and the detection reagent displaces the substance in the sample. The displaced substance only absorbs in the ultraviolet and violet light ranges. Therefore, generally only the 340nm-420nm violet or ultraviolet light mentioned above can be used, and light of other wavelengths cannot be used. In addition to using the light of the respective wavelength ranges mentioned above, the coagulation method and the immunoturbidimetric method can theoretically use light of other wavelength ranges for detection.

[0086] The applicant has studied the above-mentioned issues and proposed an alternative solution: increasing light intensity to compensate for the effects of low light flux caused by interfering substances, thereby addressing the impact of interfering substances in samples on item detection. Specifically, a specific type of measurement station is introduced to measure samples with high interfering substance content. This type of measurement station provides light with a higher intensity than the normal measurement light. By increasing the irradiation light intensity, even samples with heavy interference still have a certain amount of transmitted light that meets the requirements, the maximum absorbance test range of the sample can be increased, thereby enhancing the color interference resistance of the photodetector.

[0087] In some embodiments, a second type of measuring position (or anti-interference measuring position) can be introduced on the basis of the first type of measuring position (or conventional measuring position), wherein the first type of measuring position is provided with a first intensity of light, and the second type of measuring position is provided with a second intensity of light greater than the first intensity. The transmission light response curves of the heavy chyle sample at the anti-interference measuring position and the conventional measuring position are as follows: Figure 3 As shown, it can be seen that since the initial light intensity is weaker at the conventional measurement position, the received light flux is almost zero, but the reaction can still be detected at the anti-interference measurement position. The invention will be described in detail below.

[0088] In some embodiments of the present invention, a sample analysis device is disclosed. The sample analysis device is an instrument for analyzing and measuring samples. Let's take a coagulation analyzer (also referred to as a coagulometer in this article) as an example to illustrate the test process of the sample analysis device. The test process of the coagulation analyzer is generally as follows: a sample such as blood or plasma is added to a container such as a reaction cup, and a detection reagent is added to prepare a measurement sample (or a mixture, reaction liquid, etc.). After incubating the measurement sample, the reaction cup is placed in a preset position such as a sample measurement position. The coagulation analyzer can irradiate the measurement sample in the reaction cup with, for example, multi-wavelength light, and analyze it through coagulation method, immunoturbidimetry or chromogenic substrate method to obtain a coagulation reaction curve of the measurement sample over time, thereby further calculating the coagulation time or other coagulation-related performance parameters of the measurement sample.

[0089] Please refer to Figure 4 、 Figure 5 and Figure 6 In some embodiments, the sample analysis device may include an optical detection component 10, an illumination component 20, and a transport component 30. In some embodiments, it may also include a control component 40 and / or an analysis component 50, which will be described in detail below.

[0090] The optical detection unit 10 includes a first-type measurement site 01 and a first detector 11 corresponding to the first-type measurement site 01 , a second-type measurement site 02 and a second detector 12 corresponding to the second-type measurement site 02 .

[0091] In some embodiments, the first type measurement bit 01 is one or more. In some embodiments, the second type measurement bit 02 is one or more.

[0092] In some examples, the first detector 11 and the second detector 12 may be implemented by components capable of converting optical signals into electrical signals, such as photodetectors. Specifically, the first detector 11 and the second detector 12 may be photodiodes (PDs), photomultiplier tubes (PMTs), avalanche photodiodes (APDs), charge-coupled devices (CCDs), complementary metal-oxide semiconductors (CMOSs), image-intensified detectors (ICCDs), or electron-multiplying detectors (EMCCDs).

[0093] The optical detection component 10 is configured to cooperate with the illumination component 20. In some embodiments, the optical detection component 10 is configured to receive an output optical signal from the first container at the first-type measurement position 01 or the second-type measurement position 02 after being illuminated by the illumination component 20, and convert the output optical signal into a corresponding electrical signal. The output optical signal includes at least one of transmitted light, reflected light, or scattered light. The electrical signal is used to analyze the sample. In some embodiments, the output optical signal is transmitted light.

[0094] The illumination component 20 is used to provide light for measurement. In some embodiments, the illumination component 20 can provide two intensities of light, such as light of a first intensity and light of a second intensity, wherein the first intensity is less than the second intensity. The light of the first intensity can be the intensity of light usually used to measure samples, and the light of the second intensity is light with a stronger intensity than the first intensity, and can be used to measure samples containing interferences in the present invention. In some specific embodiments, the illumination component 20 is used to provide light of a first intensity to illuminate a first container (such as a reaction cup) located at a first type of measurement position 01 and containing a measurement sample, and to provide light of a second intensity to illuminate a first container located at a second type of measurement position 02 and containing a measurement sample; accordingly, the first detector 11 is used to obtain first optical detection information corresponding to the light of the first intensity, and the second detector 12 is used to obtain second optical detection information corresponding to the light of the second intensity; wherein the measurement sample is prepared by a sample and a reagent (or detection reagent).

[0095] The illumination component 20 may include two light sources, one light source is used to provide light of a first intensity to the first type measurement position 01 , and the other light source is used to provide light of a second intensity to the second type measurement position 02 , as described in detail below.

[0096] In some embodiments, please refer to Figure 7The illumination component 20 includes a first light source 21 and a multi-fiber bundle 22, which provide light of a first intensity to the first-type measurement station 01. In some embodiments including an interfering object detection station 03 (which will be discussed in detail below), the illumination component 20 includes a first light source 21 and a multi-fiber bundle 22, which provide light of the first intensity to the first-type measurement station 01 and the interfering object detection station 03. In some embodiments, the first light source 21 is a multi-wavelength light source, configured to provide light of at least a first wavelength, a second wavelength, and a third wavelength. In some preferred embodiments, the first wavelength ranges from 340 nm to 420 nm, the second wavelength ranges from 520 nm to 590 nm, and the third wavelength ranges from 660 nm to 800 nm. By providing multiple wavelengths of light to the first-type measurement station 01, the same first-type measurement station 01 can be used to perform multiple tests, for example, supporting tests performed using coagulation assays, immunoturbidimetry, and chromogenic substrates. In some embodiments, the first light source 21 sequentially outputs light of a first wavelength and a first intensity, light of a second wavelength and a first intensity, and light of a third wavelength and a first intensity within a predetermined illumination cycle. The above is an example of providing light of a first intensity to multiple first-type measurement locations 01 using the first light source 21 and a multi-fiber bundle 22. Each optical fiber at the output end of the multi-fiber bundle 22 corresponds to a first-type measurement location 01, thereby forming multiple first-type measurement locations 01. Because the same first light source 21 is used, each first-type measurement location 01 is essentially identical. The first light source 21 can include multiple single-wavelength light sources, thereby forming a structure capable of emitting light at multiple wavelengths. The first light source 21 can also be a broad-spectrum white light source, such as a halogen lamp or a white light lamp. For example, in conjunction with a rotatable filter, it can achieve time-sharing emission of light of different wavelengths within a cycle. In some embodiments, each first-type measurement location 01 can be equipped with a first light source 21, and each first light source 21 can provide light of a different wavelength to the corresponding first-type measurement location 01.

[0097] In some embodiments, please refer to Figure 8The illumination component 20 further includes a second light source 23 for providing light of a second intensity to illuminate the first container containing the measurement sample located at the second type measurement location 02. In some embodiments, the second light source 23 is a single-wavelength light source, configured to provide light of a fourth wavelength. In some embodiments, the fourth wavelength can be equal to any one of the first, second, or third wavelengths. In some embodiments, the fourth wavelength is not less than any one of the first, second, or third wavelengths. For example, the fourth wavelength can be 800 nm. In other embodiments, the second light source 23 can also be a multi-wavelength light source, configured to provide light of at least the first, second, and third wavelengths. In some embodiments, the second light source 23 sequentially outputs light of the first wavelength and second intensity, light of the second wavelength and second intensity, and light of the third wavelength and third intensity in a predetermined order within a single illumination cycle. To improve light performance, the second light source 23 can undergo relevant optical shaping before illuminating the second type measurement location 02.

[0098] As can be seen from the above description, the first-type measurement station 01 is provided with light of a first intensity, while the second-type measurement station 02 is provided with light of a second intensity. Generally, the first-type measurement station 01 is used to measure normal samples or samples with relatively low interfering substances. Therefore, multiple first-type measurement stations 01 are typically provided. All first-type measurement stations 01 in a sample analyzer can share a single light source via a multi-fiber optical fiber bundle. However, all first-type measurement stations 01 in a sample analyzer are also limited by this single light source. Therefore, it is difficult to adjust the light intensity emitted by this single light source to a high level (e.g., the second intensity) because this would affect the optical detection of normal samples at all first-type measurement stations 01. Therefore, setting the first-type measurement station 01 to receive light of the first intensity and introducing the second-type measurement station 02 to receive light of the second intensity is a relatively reasonable and excellent design.

[0099] The transport component 30 is used to transport the first container to the first type measurement position 01 or the second type measurement position 02. Specifically, the transport component 30 transports the first container containing the measurement sample to at least one of the first type measurement position 01 or the second type measurement position 02 for optical detection.

[0100] The control component 40 is used to control the operation of the transport component 30. For example, the control component can control the transport component 30 to transport the first container containing the measurement sample to the first type measurement position 01 or the second type measurement position 02 for optical detection based on the interference detection information of the sample.

[0101] The analyzing component 50 is used to analyze the electrical signal output by the optical detecting component to analyze the sample detection items. In some embodiments, the analyzing component 50 is used to analyze the detection results of the sample based on at least one of the first optical detection information or the second optical detection information.

[0102] In the present invention, a first container containing a measurement sample can be transported to a first-type measurement station 01 and a second-type measurement station 02 for optical detection, respectively. Then, based on preset conditions, such as conditions related to sample interfering substance detection information, at least one of the first optical detection information and the second optical detection information is selected to analyze the test results of the sample. Alternatively, based on preset conditions, such as conditions related to sample interfering substance detection information, the first container containing the measurement sample can be selectively transported to either the first-type measurement station 01 or the second-type measurement station 02 for optical detection.

[0103] In some embodiments, the interfering substance detection information includes at least one of the sample's absorbance at different wavelengths or its transmittance. Taking absorbance as an example, the sample's absorbance at different wavelengths represents the degree of light absorption by the sample when the sample is illuminated with light. If the sample's absorbance at a preset wavelength exceeds a preset absorbance threshold, for example, the absorbance at at least one of 405nm, 575nm, 660nm, and 800nm ​​exceeds the corresponding absorbance threshold, then the interfering substance in the sample exceeds the interfering substance threshold. Taking transmittance as an example, the sample's transmittance represents the degree to which light can pass through the sample when illuminated with light. The transmittance of the sample can be the initial light flux detected before the sample is formally tested for coagulation. If the initial light flux of the sample is lower than the preset light flux threshold, then the interfering substance in the sample exceeds the interfering substance threshold.

[0104] It can be seen that no matter which method is used, it involves the detection of interference substances in the sample, so the following describes how to detect interference substances in the sample.

[0105] Please refer to Figure 9 In some embodiments, the sample analysis device may further include an interference detection component 60, which is used to detect interferences on the sample to obtain interference detection information, and the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold. There are many ways to implement the interference detection component 60, for example, please refer to Figure 10The interference detection component 60 may include an interference detection position 03 and a third detector 13 located on one side of the interference detection position. The third detector 13 may be implemented by a component capable of converting an optical signal into an electrical signal, such as a photodetector. Specifically, the third detector 13 may be a photodiode PD, a photomultiplier tube PMT, an avalanche photodiode APD, a charge-coupled device CCD, a complementary metal oxide semiconductor CMOS, an image intensification detector ICCD, or an electron multiplication type EMCCD. The illumination component 20 is used to illuminate a second container (such as a reaction cup or a colorimetric cell, etc.) located at the interference detection position and containing at least a sample - for example, the illumination component 20 illuminates with light of a first intensity; the third detector 13 is used to receive the output optical signal of the above-mentioned second container after being illuminated by the illumination component 20, so as to obtain interference detection information of the sample to be tested.

[0106] The introduction of the interference detection component 60 can detect interferences in the sample to be tested and obtain sample interference detection information. In other embodiments, the interference detection bit can be omitted and the interference detection bit can be used to detect interferences in the sample to be tested. The details are as follows.

[0107] In some embodiments, the illumination component 20 illuminates a first container containing a measurement sample at a first-type measurement position 01 with light of a first intensity. The first detector 11 is configured to receive an output light signal from the first container after being illuminated by the illumination component 20, thereby obtaining interfering substance detection information for the sample under test. This interfering substance detection information is used to determine whether the interfering substance in the sample under test exceeds a threshold. Specifically, the interfering substance detection information can be obtained by utilizing the average light flux during the period between the first container containing the measurement sample being placed in the first-type measurement position 01 and the start of testing. In one example, timing begins after the last step of adding the sample to trigger the reagent. Generally, mixing the mixture and moving it to the first-type measurement position 01 can be completed within 3 seconds, and testing begins at the 10th second. During the 7 seconds between the 3rd and 10th seconds, the illumination component 20 illuminates the first container containing the measurement sample at the sample measurement position with light of a first intensity. The first detector 11 is configured to receive an output light signal from the first container after being illuminated by the illumination component 20, such as the average light flux during this period and the minimum light transmittance, to obtain interfering substance detection information for the sample under test.

[0108] The above are some embodiments of detecting sample interfering substances. It is understandable that those skilled in the art may also use other methods to detect sample interfering substances, such as by taking a photo of the sample to be tested, obtaining an image of the sample to be tested, and then analyzing the image through methods such as machine learning to obtain interfering substance detection information of the sample to be tested.

[0109] The above describes some basic structures of the sample analysis device. Please refer to Figure 11 , the sample analysis device of some embodiments also includes other components and structures, which are described in detail below.

[0110] The sample unit 80 is used to carry the sample. In some examples, the sample unit 80 may include a sample delivery module (SDM) and a front track; in other examples, the sample unit 10 may also be a sample tray, such as Figure 11 For example, the sample tray includes a plurality of sample positions for placing containers. The sample tray can dispatch the samples to corresponding positions, such as the position for the dispensing component 70 to absorb the samples, by rotating its tray structure.

[0111] The reagent unit 82 is used to carry reagents. In one embodiment, the reagent unit 82 is arranged in a disc-shaped structure and has multiple positions for carrying reagent containers. The reagent unit 82 can rotate and drive the reagent containers it carries to rotate, and is used to rotate the reagent containers to the reagent aspiration position for the dispensing component 70 to aspirate the reagent. The number of reagent units 82 can be one or more.

[0112] The dispensing component 70 is used to absorb samples or reagents and discharge them. In some embodiments, the dispensing component 70 may include a sample dispensing component 71 and / or a reagent dispensing component 73. The sample dispensing component 71 is used to absorb samples and discharge them into a container to be added, such as a first container. In some embodiments, the sample dispensing component 71 may include a sample needle, which performs two-dimensional or three-dimensional motion in space through a two-dimensional or three-dimensional driving mechanism, so that the sample needle can move to absorb the sample carried by the sample unit 80, and move to a position to be added, such as a first container, and discharge the sample into the first container. In some embodiments, the reagent dispensing component 73 may include a reagent needle, which performs two-dimensional or three-dimensional motion in space through a two-dimensional or three-dimensional driving mechanism, so that the reagent needle can move to absorb the reagent carried by the reagent unit 82, and move to a position to be added, such as a first container, and discharge the reagent into the first container. In some embodiments, the sample dispensing component 71 and the reagent dispensing component 73 may also share a set of driving mechanisms and needles. When the sample dispensing component 71 and the reagent dispensing component 73 share a set of driving mechanisms and needles, the needles need to be cleaned when aspirating samples and reagents to avoid cross-contamination and other problems.

[0113] Figure 4The reaction assembly 84 is used to hold a test specimen prepared from a sample and detection reagents. In one example, the reaction assembly 84 is a disc-shaped structure with multiple placement locations for first containers, such as cuvettes. The reaction assembly 84 is capable of rotating, driving the cuvettes within these placement locations to rotate, allowing for the placement of cuvettes within the reaction disk and the incubation of the mixture within them. The first-type test locations 01 and the second-type test locations 02 can be located on the reaction assembly 84, with some of the placement locations on the reaction assembly 84 designated as first-type test locations 01 and second-type test locations 02. Alternatively, the first-type test locations 01 and second-type test locations 02 can be located independently of the reaction assembly 84, for example, at a location near the reaction assembly 84.

[0114] The following is an explanation of the test process of the sample analysis device.

[0115] In some embodiments having an interference detection component 60, the control component 40 can control a dispensing component 70 to dispense a portion of the sample, or a diluent, into a second container (e.g., a colorimetric cell). The illumination component 20 illuminates the second container located at the interference detection position 03 and containing at least the sample (the sample, or a mixture of the sample and the diluent). The interference detection component 60 is configured to receive the output light signal from the second container after being illuminated by the illumination component 20 to obtain interference detection information about the sample. The following method 1 or method 2 can then be performed.

[0116] In Method 1, the control unit 40 controls the dispensing unit 70 to dispense another portion of the sample and the detection reagent into the first container to prepare the measurement sample. If the interfering substance content of the sample does not exceed the threshold, the control unit 40 controls the transport unit 30 to transport the first container to the first-type measurement position 01 for optical testing. If the interfering substance content of the sample exceeds the threshold, the control unit 40 controls the transport unit 30 to transport the first container to the second-type measurement position 02 for optical testing.

[0117] In the second method, the control unit 40 controls the dispensing unit 70 to dispense another portion of the sample and the detection reagent into the first container to prepare the measurement sample. The transport unit 30 sequentially transports the first container to the first-type measurement position 01 for optical detection to obtain first optical detection information, and then to the second-type measurement position 02 for optical detection to obtain second optical detection information. If the interfering substance content of the sample does not exceed the threshold, the analysis unit 50 selects the first optical detection information to analyze the sample test results. If the interfering substance content of the sample exceeds the threshold, the analysis unit 50 selects the second optical detection information to analyze the sample test results.

[0118] In an embodiment where a sample is tested for interfering substances at a first-type measurement station 01, an illumination unit 20 illuminates a first container containing a measurement sample at the first-type measurement station 01 with light of a first intensity. A first detector 11 receives the optical signal output by the illumination unit 20 from the first container to obtain interfering substance detection information for the sample. If the interfering substance detection information determines that the interfering substance content does not exceed a threshold, the measurement sample is optically tested at the first-type measurement station 01. If the interfering substance content exceeds the threshold, the transport unit 30 transports the first container to the second-type measurement station 02 for optical testing.

[0119] The sample herein may be a blood sample, and the interfering substance may include at least one of hemoglobin, bilirubin, and chyle. The interfering substance detection information herein may include at least one of the sample's absorbance of light at different wavelengths or its transmittance. For example, in an embodiment in which interfering substance detection is performed on a sample at a first-type measurement position 01, the illumination component 20 illuminates a first container containing a measurement sample at the first-type measurement position 01 with light of a first intensity, and the first detector 11 receives the output light signal of the first container after being illuminated by the illumination component 20 to obtain the luminous flux of the measurement sample; when the luminous flux of the measurement sample exceeds a threshold, the measurement sample is optically detected at the first-type measurement position 01; conversely, if the luminous flux of the measurement sample does not exceed the threshold, the transport component 30 transports the first container to the second-type measurement position 02 for optical detection.

[0120] The above is some description of the sample analysis device. In some embodiments of the present invention, a method for sample analysis is also disclosed, which provides a first type of measurement position and a second type of measurement position. Figure 12 In some embodiments, the sample analysis method includes the following steps:

[0121] Step 110: Dispense the sample and reagent into the first container to prepare a test specimen.

[0122] Step 120: Transport the first container to a first type measurement location and / or a second type measurement location.

[0123] Step 130: Irradiate the first type of measurement position with light of a first intensity, and / or irradiate the second type of measurement position with light of a second intensity; the first intensity is less than the second intensity.

[0124] Step 140: Obtain optical detection information corresponding to the light of the first intensity and / or the light of the second intensity.

[0125] Step 150: Analyze the detection result of the sample according to the optical detection information corresponding to the light of the first intensity and / or the light of the second intensity.

[0126] The following details the further processes and relationships between the steps.

[0127] Please refer to Figure 13 In some embodiments, the sample analysis method includes the following steps:

[0128] Step 100: Before the first container is transported to the first type measurement position and / or the second type measurement position in the following step 120, the sample is tested for interference to obtain interference detection information of the sample, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value. Figure 14 Step 100 of performing interference detection on the sample to obtain interference detection information of the sample includes: step 101, injecting a portion of the sample and a diluent into a second container; step 102, irradiating the second container with light of a first intensity; step 103, receiving an output light signal after irradiating the second container; step 104, converting the light signal into an electrical signal; and step 105, analyzing the electrical signal to obtain interference detection information of the sample.

[0129] Step 110: Dispense the sample and reagent into the first container to prepare a measurement sample. For example, step 110 dispenses the reagent and the remaining sample from step 101 into the first container to prepare a measurement sample.

[0130] Step 120: Transport the first container to a first-type measurement station and / or a second-type measurement station. Specifically, if the interfering substance content of the sample does not exceed a threshold, step 121 transports the first container to the first-type measurement station for optical detection. If the interfering substance content of the sample exceeds the threshold, step 122 transports the first container to the second-type measurement station for optical detection.

[0131] If in step 120 the first container is transported to the first type measurement position for optical inspection, then in step 130 light of a first intensity is irradiated on the first type measurement position, and optical detection information corresponding to the light of the first intensity is obtained in step 140, and in step 150 the detection result of the sample is analyzed based on the optical detection information corresponding to the light of the first intensity.

[0132] If in step 120 the first container is transported to the second type measurement position for optical inspection, then in step 130 light of a second intensity is irradiated on the second type measurement position, and optical detection information corresponding to the light of the second intensity is obtained in step 140, and in step 150 the detection results of the sample are analyzed based on the optical detection information corresponding to the light of the second intensity.

[0133] Please refer to Figure 15In some embodiments, the sample analysis method includes the following steps:

[0134] Step 110: Dispense the sample and reagent into the first container to prepare a test specimen.

[0135] Step 121: Transport the first container to a first type measurement location.

[0136] Step 122: After transporting the first container to the first type of measurement position, obtain interference detection information of the measurement sample measured at the first type of measurement position, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold.

[0137] Step 123: If the interfering substance content of the sample does not exceed the threshold, optical detection of the measurement sample in the first container is performed at the first type measurement position. Step 130 irradiates the first type measurement position with light of a first intensity, and in step 140 obtains optical detection information corresponding to the first intensity of light. In step 150, the detection result of the sample is analyzed based on the optical detection information corresponding to the first intensity of light.

[0138] Step 125: If the interfering substance content of the sample exceeds the threshold, the first container is moved from the first type of measurement station to the second type of measurement station for optical testing. Step 130 irradiates the second type of measurement station with light of a second intensity, and in step 140 obtains optical detection information corresponding to the light of the second intensity. In step 150, the sample is analyzed for detection results based on the optical detection information corresponding to the light of the second intensity.

[0139] Please refer to Figure 16 In some embodiments, the sample analysis method includes the following steps:

[0140] Step 110: Dispense the sample and reagent into the first container to prepare a test specimen.

[0141] Step 120: Transport the first container to the first type measurement position and / or the second type measurement position. Specifically, step 120 transports the first container to the first type measurement position for optical measurement and transports the first container to the second type measurement position for optical measurement.

[0142] Step 130: Irradiate the first type of measurement position with light of a first intensity, and irradiate the second type of measurement position with light of a second intensity; the first intensity is less than the second intensity.

[0143] Step 140: Obtain optical detection information corresponding to the light of the first intensity and the light of the second intensity.

[0144] Step 150: Analyze the test results of the sample based on the optical detection information corresponding to the first and second intensity light. Specifically, step 150 selects the optical detection information measured at the first or second type of measurement position based on the interferent detection information of the sample. For example, if the interferent content of the sample does not exceed a threshold, step 150 selects the optical detection information measured at the first type of measurement position; if the interferent content of the sample exceeds the threshold, step 150 selects the optical detection information measured at the second type of measurement position. Step 150 then analyzes the test results of the sample based on the selected optical detection information.

[0145] The above is an explanation of the sample analysis methods according to some embodiments of the present invention.

[0146] This document is described with reference to various exemplary embodiments. However, those skilled in the art will recognize that changes and modifications may be made to the exemplary embodiments without departing from the scope of this document. For example, the various operational steps and components used to perform the operational steps may be implemented in different ways (e.g., one or more steps may be deleted, modified, or incorporated into other steps) depending on the specific application or considering any number of cost functions associated with the operation of the system.

[0147] In the above embodiments, all or part of the embodiments may be implemented through software, hardware, firmware, or any combination thereof. In addition, as will be appreciated by those skilled in the art, the principles of this invention may be embodied in a computer program product on a computer-readable storage medium pre-installed with computer-readable program code. Any tangible, non-transitory computer-readable storage medium may be used, including magnetic storage devices (hard disks, floppy disks, etc.), optical storage devices (CD-ROMs, DVDs, Blu-ray discs, etc.), flash memory, and / or the like. These computer program instructions may be loaded onto a general-purpose computer, a special-purpose computer, or other programmable data processing device to form a machine, such that the instructions executed on the computer or other programmable data processing component may generate a component that implements a specified function. These computer program instructions may also be stored in a computer-readable memory, which may instruct the computer or other programmable data processing device to operate in a specific manner, such that the instructions stored in the computer-readable memory may form an article of manufacture, including an implementation component that implements a specified function. The computer program instructions may also be loaded onto a computer or other programmable data processing device, thereby causing the computer or other programmable device to execute a series of operational steps to generate a computer-implemented process, such that the instructions executed on the computer or other programmable device may provide the steps for implementing the specified function.

[0148] Although the principles of this invention have been shown in various embodiments, many modifications of structure, arrangement, proportion, elements, materials and components that are particularly suitable for specific environments and operational requirements can be used without departing from the principles and scope of this invention. The above modifications and other changes or amendments are intended to be included within the scope of this invention.

[0149] The foregoing detailed description has been described with reference to various embodiments. However, those skilled in the art will recognize that various modifications and changes can be made without departing from the scope of this disclosure. Therefore, the present disclosure will be considered in an illustrative rather than a restrictive sense, and all such modifications will be included within its scope. Similarly, the advantages, other advantages and solutions to the problems of the various embodiments have been described above. However, the benefits, advantages, solutions to the problems and any elements that can produce these, or make them more specific, should not be interpreted as critical, required or necessary. The term "comprising" and any other variants used in this article are all non-exclusive inclusions, so that a process, method, article or device that includes a list of elements includes not only these elements, but also other elements that are not explicitly listed or do not belong to the process, method, system, article or device. In addition, the term "coupled" and any other variants used in this article refer to physical connections, electrical connections, magnetic connections, optical connections, communication connections, functional connections and / or any other connections.

[0150] Those skilled in the art will recognize that many changes can be made to the details of the above embodiments without departing from the basic principles of the invention. Therefore, the scope of the present invention should be determined solely by the claims.

Claims

1. A sample analysis device, characterized in that: include: An optical detection component includes a first type of measurement position and a first detector corresponding to the first type of measurement position, a second type of measurement position and a second detector corresponding to the second type of measurement position; an illumination component for providing light of a first intensity to illuminate the first container located at the first type measurement position and containing the measurement sample, and providing light of a second intensity to illuminate the first container located at the second type measurement position and containing the measurement sample; The assay sample is prepared from a sample and a reagent; the first intensity is less than the second intensity; a transporting component, configured to transport the first container to the first type of measurement location or the second type of measurement location; The control component is used to control the transport component to transport the first container containing the measurement sample to the first type measurement position or the second type measurement position for optical detection based on the interference detection information of the sample.

2. The sample analysis device according to claim 1, wherein The optical detection component is used to receive an output light signal after the first container at the first type measurement position or the second type measurement position is illuminated by the illumination component, and convert the output light signal into a corresponding electrical signal; the output light signal includes at least one of transmitted light, reflected light or scattered light; The sample analysis device further includes an analysis component for analyzing the electrical signal to perform analysis on the sample detection items.

3. The sample analysis device according to claim 1 or 2, wherein: It also includes an interference detection component for performing interference detection on the sample to obtain the interference detection information, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value, and the control component is used to control the transport component to transport the first container to the first type of measurement position when the interference content of the sample does not exceed the threshold value; or to control the transport component to transport the first container to the second type of measurement position when the interference content of the sample exceeds the threshold value.

4. The sample analysis device according to claim 3, wherein: The interference detection component includes an interference detection position and a third detector located on one side of the interference detection position; the illumination component is used to illuminate a second container located at the interference detection position and containing at least a sample, and the interference detection component is used to receive an output light signal of the second container after being illuminated by the illumination component to obtain interference detection information of the sample.

5. The sample analysis device according to claim 4, wherein: The apparatus further includes a dispensing component, wherein the control component is configured to control the dispensing component to dispense a portion of the sample and the diluent into the second container, and to control the dispensing component to dispense another portion of the sample and the detection reagent into the first container, so as to prepare the measurement sample.

6. The sample analysis device according to claim 4 or 5, wherein: The first container is a reaction cup, and the second container is a colorimetric cell.

7. The sample analysis device according to claim 4, wherein: The illumination component includes a first light source and a multi-fiber optical fiber bundle, which respectively provide light of the first intensity to the first type measurement position and the interference object detection position.

8. The sample analysis device according to claim 5, wherein: The illumination component includes a first light source and a multi-fiber optical fiber bundle, which respectively provide light of the first intensity to the first type measurement position and the interference object detection position.

9. The sample analysis device according to claim 6, wherein: The illumination component includes a first light source and a multi-fiber optical fiber bundle, which respectively provide light of the first intensity to the first type measurement position and the interference object detection position.

10. The sample analysis device according to claim 1, 7, 8 or 9, wherein: The illumination component further includes a second light source configured to provide light of the second intensity to illuminate the first container located at the second type of measurement position and containing the measurement sample.

11. The sample analysis device according to claim 10, wherein: The first light source is a multi-wavelength light source for providing light of at least a first wavelength, a second wavelength, and a third wavelength.

12. The sample analysis device according to claim 11, wherein: The first wavelength range is 340nm-420nm, the second wavelength range is 520nm-590nm, and the third wavelength range is 660nm-800nm.

13. The sample analysis device according to claim 11, wherein: The second light source is a single-wavelength light source, configured to provide light of a fourth wavelength, where the fourth wavelength is not less than any one of the first wavelength, the second wavelength, or the third wavelength.

14. The sample analysis device according to claim 1, wherein: The illumination component illuminates the first container containing the measurement sample at the first type measurement position with light of a first intensity, and the first detector is used to receive the output light signal of the first container after being illuminated by the illumination component to obtain interference detection information of the sample.

15. The sample analysis device according to claim 1, wherein: The sample is a blood sample, and the interferent includes at least one of hemoglobin, bilirubin, and chyle.

16. The sample analysis device according to claim 1, 4 or 14, wherein: The interference detection information includes at least one of light absorbance or light transmittance of the sample at different wavelengths.

17. The sample analysis device according to claim 3, wherein: The interference detection information includes at least one of light absorbance or light transmittance of the sample at different wavelengths.

18. A method for sample analysis, characterized in that: include: Providing a first type of measurement bit and a second type of measurement bit; wherein the first type of measurement bit is illuminated by light of a first intensity, and the second type of measurement bit is illuminated by light of a second intensity, wherein the first intensity is less than the second intensity; Dispensing the sample and reagent into a first container to prepare a test specimen; The first container containing the measurement sample is controlled to be transported to the first type measurement position or the second type measurement position for optical detection based on the interferent detection information of the sample.

19. The method according to claim 18, wherein The method further includes, before transporting the first container to the first type of measurement position or the second type of measurement position, performing interference detection on the sample to obtain interference detection information of the sample, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value; and controlling the transport of the first container containing the measurement sample to the first type of measurement position or the second type of measurement position for optical detection based on the interference detection information of the sample, comprising: If the interferent content of the sample does not exceed the threshold, transporting the first container to the first type of measurement site; or If the interferent content of the sample exceeds a threshold, the first container is transported to the second type of measurement site.

20. The method according to claim 19, wherein The performing interference detection on the sample to obtain the interference detection information of the sample includes: Dispensing a portion of the sample and the diluent into a second container; irradiating the second container with light of a first intensity; receiving an output light signal after irradiating the second container; converting the optical signal into an electrical signal; The electrical signal is analyzed to obtain interference detection information of the sample.

21. The method of claim 18, wherein: The method further includes transporting the first container to the first type of measurement position to obtain the interference detection information of the measurement sample measured at the first type of measurement position, wherein the interference detection information is used to indicate whether the interference content of the sample exceeds a threshold value; and controlling the transport of the first container containing the measurement sample to the first type of measurement position or the second type of measurement position for optical detection based on the interference detection information of the sample includes: If the interferent content of the sample exceeds a threshold, the first container is transported from the first type of measurement site to the second type of measurement site.

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