Microfluidic product plate for screening tumor cells using immunomagnetic beads

Abstract

This invention discloses a microfluidic product plate for screening tumor cells using immunomagnetic beads, belonging to the field of tumor cell screening technology. It includes a dust-free injection-molded or micro-injection-molded product core plate with a pipeline processing mechanism mounted on it for screening target cells in injected liquid. The pipeline processing mechanism includes an input pipe, several delivery pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe, and an output pipe. The input pipe is connected to a sample injection pipe, a sheath flow injection pipe, an immunomagnetic bead injection pipe, and a blood cell recovery pipe. The output pipe is connected to a second sheath flow injection pipe, a waste liquid recovery pipe, and a target cell recovery pipe. The first sheath flow injection pipe, the immunomagnetic bead injection pipe, the blood cell recovery pipe, the electromagnet, and the second sheath flow injection pipe work together to separate impurity cells at the front end of the same product core plate, inject and mix immunomagnetic beads in the middle, and precisely sort target cells at the rear end.

Description

Technical Field

[0001] This invention belongs to the field of tumor cell screening technology, specifically relating to a microfluidic product plate that uses immunomagnetic beads to screen tumor cells. Background Technology

[0002] Microfluidics refers to the science and technology involved in systems that use microchannels to process or manipulate tiny fluids. It is an emerging interdisciplinary field involving chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering. Due to its miniaturization and integration characteristics, microfluidic devices are often called microfluidic chips, also known as lab-on-a-chip systems and micro total analysis systems. One of the key characteristics of microfluidics is its unique fluid properties at the microscale, such as laminar flow and droplet flow. Utilizing these unique fluid phenomena, microfluidics can achieve a range of microfabrications and micromanipulations that are difficult to accomplish using conventional methods. Currently, microfluidics is considered to have enormous development potential and broad application prospects in biomedical research.

[0003] Currently, there are not many rapid clinical testing methods in the domestic market that use immunomagnetic beads to screen tumor cells; in addition, there are no products that can complete the injection and mixing of magnetic beads and reuse electromagnetic precision sorting on the same microfluidic chip.

[0004] Compared to commonly used methods such as bioelectrode screening and fluorescence-based precise localization in laboratories abroad, both place extremely high demands on the fabrication of the carriers. Given my country's large population and user base, two major challenges exist in clinical testing and in vitro diagnostic applications: timeliness and cost.

[0005] For example, the bioelectrode screening method requires vacuum sputtering of multiple metal films on a glass substrate; then photolithography is used to reveal the electrode pattern. The process is complex, costly, and not conducive to mass production. For example, the fluorescence precise labeling method requires creating a dense array of concave holes on a glass substrate according to the size of the target cells using photolithography or laser micro-engraving. The general process is complex, costly, and not conducive to mass production. Summary of the Invention

[0006] The purpose of this invention is to provide a microfluidic product plate for screening tumor cells using immunomagnetic beads, in order to solve the problems mentioned in the background art, such as the complexity, high cost, and difficulty in mass production of existing bioelectrode screening and fluorescence precise positioning methods, and the inability to continuously perform magnetic bead injection and magnetic bead mixing and reuse for electromagnetic precise sorting.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a microfluidic product plate for screening tumor cells using immunomagnetic beads, comprising a product core plate formed by dust-free injection molding or micro-injection molding;

[0008] The pipeline processing mechanism, installed on the product core plate, is used to screen target cells in the injected liquid. The pipeline processing mechanism includes an input pipe, several delivery pipes, a first connecting pipe, an electromagnetic adsorption unit, a second connecting pipe, and an output pipe. The input pipe is connected to a sample injection pipe, a sheath flow injection pipe, an immunomagnetic bead injection pipe, and a blood cell recovery pipe. The output pipe is connected to a second sheath flow injection pipe, a waste liquid recovery pipe, and a target cell recovery pipe. The input pipe is connected to the foremost delivery pipe, and the last delivery pipe is connected to one end of the first connecting pipe.

[0009] A protective cover is fixed to the product core board and covers the outside of the electromagnetic adsorption unit. A sealed cavity is formed between the protective cover and the product core board. The sealed cavity is connected to connecting pipe one and connecting pipe two respectively. The other end of connecting pipe two is connected to the output pipe.

[0010] Using the above scheme, by setting up a sheath flow injection tube 1, an immunomagnetic bead injection tube, a blood cell recovery tube, an electromagnet, and a sheath flow injection tube 2 in combination, the separation of impurity cells is completed at the front end of the same product core plate, the injection and mixing of immunomagnetic beads are completed in the middle, and the precise sorting of target cells is completed at the rear end. By setting up the immunomagnetic bead injection tube in combination, the same product core plate can be used to screen different target cells by injecting different immunomagnetic beads, and the electromagnet can be used to achieve the screening operation. Using traditional dust-free injection molding process and micro-injection molding process to produce product core plates, the production and packaging of finished products can be completed quickly and in large quantities, with low cost and simple process structure.

[0011] In a preferred embodiment, the sample injection tube, sheath flow injection tube one, immunomagnetic bead injection tube, and sheath flow injection tube two are all arranged vertically upwards, while the blood cell recovery tube, target cell recovery tube, and waste liquid recovery tube are all arranged vertically downwards.

[0012] Using the above scheme, the upward-facing sample injection tube facilitates the injection of sample solution, the sheath flow injection tube and the immunomagnetic bead injection tube facilitate the injection of sheath flow and immunomagnetic beads, the downward-facing blood cell recovery tube facilitates the rapid recovery of blood cells, and the target cell recovery tube and the waste liquid recovery tube facilitate the recovery of target cells and waste liquid.

[0013] In a preferred embodiment, the conveying pipes are specifically arranged in a serpentine pattern, and the ends of several serpentine conveying pipes are interconnected.

[0014] The above scheme utilizes a serpentine layout of delivery pipes, with multiple delivery pipes connected end-to-end, to facilitate the thorough delivery and mixing of the liquid.

[0015] In a preferred embodiment, the electromagnetic adsorption unit includes an electromagnet, which is mounted on the product core board and electrically connected to an external power source.

[0016] Using the above scheme, electromagnets can be used in conjunction with immunomagnetic beads, which move in a directional manner under the magnetic influence of the electromagnets, thereby achieving the purpose of separating viral factors.

[0017] In a preferred embodiment, the sheath flow injection tube and the immunomagnetic bead injection tube are located on the front and rear sides of the blood cell recovery tube, respectively.

[0018] By using the above scheme, and placing the blood cell recovery tube between the sheath flow injection tube and the immunomagnetic bead injection tube, the separation of impurity cells and the injection and mixing of immunomagnetic beads in the middle can be completed at the front end of the same product core plate.

[0019] In a preferred embodiment, the protective cover is filled with nitric oxide gas.

[0020] By using the above method, nitric oxide gas is filled inside the protective shield, which can prevent external gases from entering the shield and achieve a good isolation and protection effect.

[0021] Compared with the prior art, the beneficial effects of the present invention are:

[0022] This microfluidic product plate for screening tumor cells using immunomagnetic beads is designed with a sheath flow injection tube (I), an immunomagnetic bead injection tube, a blood cell recovery tube, an electromagnet, and a sheath flow injection tube (II) working together. Impurity cells are separated at the front end of the same product core plate, immunomagnetic beads are injected and mixed in the middle, and target cells are precisely sorted at the rear end.

[0023] This microfluidic product plate that uses immunomagnetic beads to screen tumor cells can be used in conjunction with an immunomagnetic bead injection tube. With the same product core plate, different immunomagnetic beads can be injected to target different cells and work with an electromagnet to perform the screening operation.

[0024] This microfluidic product board, which uses immunomagnetic beads to screen tumor cells, utilizes traditional dust-free injection molding and micro-injection molding processes to produce the product core board. This allows for rapid and large-scale production and packaging of finished products, with low cost and a simple process structure. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of the present invention;

[0026] Figure 2 This is a structural schematic diagram of the present invention from another angle;

[0027] Figure 3 This is a schematic diagram of the structure of the protective cover of the present invention;

[0028] Figure 4This is a schematic diagram of the pipeline processing mechanism of the present invention.

[0029] In the diagram: 1. Product core board; 2. Pipeline processing mechanism; 3. Protective cover; 4. Input tube; 5. Sample injection tube; 6. Sheath flow injection tube one; 7. Immunomagnetic bead injection tube; 8. Blood cell recovery tube; 9. Delivery tube; 10. Connecting tube one; 11. Electromagnet; 12. Connecting tube two; 13. Output tube; 14. Sheath flow injection tube two; 15. Target cell recovery tube; 16. Waste liquid recovery tube. Detailed Implementation

[0030] Please see Figure 1-4 The present invention provides a microfluidic product plate for screening tumor cells using immunomagnetic beads, including a product core plate 1 formed by dust-free injection molding or micro-injection molding.

[0031] The pipeline processing mechanism 2, installed on the product core plate 1, is used for target cell screening of the injected liquid. The pipeline processing mechanism 2 includes an input pipe 4, several delivery pipes 9, a connecting pipe 10, an electromagnetic adsorption unit, a connecting pipe 2 12, and an output pipe 13. The sample injection pipe 5, the sheath flow injection pipe 1 6, the immunomagnetic bead injection pipe 7, and the sheath flow injection pipe 2 14 are all arranged vertically upwards. The blood cell recovery pipe 8, the target cell recovery pipe 15, and the waste liquid recovery pipe 16 are all arranged vertically downwards. The upward-arranged sample injection pipe 5 facilitates the injection of sample liquid, the sheath flow injection pipe 1 6 and the immunomagnetic bead injection pipe 7 facilitate the injection of sheath flow and immunomagnetic beads, the downward-arranged blood cell recovery pipe 8 facilitates the rapid recovery of blood cells, and the target cell recovery pipe 15 and the waste liquid recovery pipe 16 facilitate the recovery of target cells and waste liquid.

[0032] The electromagnetic adsorption unit includes an electromagnet 11, which is mounted on the product core board 1 and electrically connected to an external power source. When used in conjunction with the electromagnet 11, the electromagnetic adsorption force can be used in conjunction with immunomagnetic beads. The immunomagnetic beads move in a directional manner under the magnetic action of the electromagnet 11, thereby achieving the purpose of separating viral factors.

[0033] The sheath flow injection tube 6 and the immunomagnetic bead injection tube 7 are located on the front and rear sides of the blood cell recovery tube 8, respectively. By placing the blood cell recovery tube 8 between the sheath flow injection tube 6 and the immunomagnetic bead injection tube 7, the separation of impurity cells and the injection and mixing of immunomagnetic beads in the middle can be completed at the front end of the same product core plate 1.

[0034] The input tube 4 is connected to the sample injection tube 5, the sheath flow injection tube 1 6, the immunomagnetic bead injection tube 7, and the blood cell recovery tube 8. The output tube 13 is connected to the sheath flow injection tube 2 14, the waste liquid recovery tube 16, and the target cell recovery tube 15. The input tube 4 is connected to the foremost delivery tube 9, and the last delivery tube 9 is connected to one end of the connecting tube 1 10.

[0035] The delivery pipe 9 is specifically arranged in a serpentine pattern, and several delivery pipes 9 arranged in a serpentine pattern are connected end to end. By using the serpentine arrangement of delivery pipes 9 and the cooperation of multiple delivery pipes 9 to connect end to end, it is convenient to fully deliver and mix the liquid.

[0036] The protective cover 3 is fixed on the product core plate 1 and covers the outside of the electromagnetic adsorption unit. The protective cover 3 and the product core plate 1 form a sealed cavity, and the sealed cavity is connected to the connecting pipe 10 and the connecting pipe 12 respectively. The other end of the connecting pipe 12 is connected to the output pipe 13. The protective cover 3 is filled with nitric oxide gas. By filling the protective cover 3 with nitric oxide gas, external gas can be prevented from entering the interior of the protective cover 3, which achieves a good isolation and protection effect.

[0037] In use, the liquid to be screened is injected through the sample injection tube 5. After injection, sheath fluid is added through the sheath flow injection tube 6. Blood cells are discharged through the blood cell recovery tube 8. The required immunomagnetic beads are added through the immunomagnetic bead injection tube 7. The liquid is then transported through the input tube 4 to the delivery tube 9, and then through the delivery tube 9 and the connecting tube 10 to the inside of the protective cover 3. The electromagnet 11 works to generate a magnetic force, which drives the immunomagnetic beads to move and complete the screening operation. The liquid is then transported again through the connecting tube 2 12 and the output tube 13. Sheath fluid is injected again through the sheath flow injection tube 2 14. Waste liquid is discharged through the waste liquid recovery tube 16, and target cells are discharged through the target cell recovery tube 15.

Claims

1. A microfluidic product plate for screening tumor cells using immunomagnetic beads, characterized in that: Product core boards (1) including dust-free injection molding or micro-injection molding. The pipeline processing mechanism (2), installed on the product core plate (1), is used to perform target cell screening on the injected liquid. The pipeline processing mechanism (2) includes an input pipe (4), several delivery pipes (9), a connecting pipe one (10), an electromagnetic adsorption unit, a connecting pipe two (12), and an output pipe (13). The input pipe (4) is connected to and installed with a sample injection pipe (5), a sheath flow injection pipe one (6), an immunomagnetic bead injection pipe (7), and a blood cell recovery pipe (8). The output pipe (13) is connected to and installed with... The sheath flow injection tube 2 (14), waste liquid recovery tube (16) and target cell recovery tube (15) are connected. The input tube (4) is connected to the foremost delivery tube (9), and the last delivery tube (9) is connected to one end of the connecting tube 1 (10). The sample injection tube (5), sheath flow injection tube 1 (6), immunomagnetic bead injection tube (7) and sheath flow injection tube 2 (14) are all arranged vertically upward. The blood cell recovery tube (8), target cell recovery tube (15) and waste liquid recovery tube (16) are all arranged vertically downward. The protective cover (3) is fixed on the product core plate (1) and covers the outside of the electromagnetic adsorption unit. The protective cover (3) and the product core plate (1) form a closed cavity, and the closed cavity is connected to the first connecting pipe (10) and the second connecting pipe (12) respectively. The other end of the second connecting pipe (12) is connected to the output pipe (13).

2. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, characterized in that: The conveying pipe (9) is specifically arranged in a serpentine pattern, and the heads and tails of several serpentine conveying pipes (9) are connected to each other.

3. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, characterized in that: The electromagnetic adsorption unit includes an electromagnet (11), which is mounted on the product core board (1) and electrically connected to an external power source.

4. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, characterized in that: The sheath flow injection tube (6) and the immunomagnetic bead injection tube (7) are located on the front and rear sides of the blood cell recovery tube (8), respectively.

5. The microfluidic product plate for screening tumor cells using immunomagnetic beads according to claim 1, characterized in that: The protective cover (3) is filled with nitric oxide gas.

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