A highly efficient and continuous magnetic bead mother solution preparation device

Abstract

The present invention provides a highly efficient and continuous magnetic bead mother liquor preparation device, which belongs to the field of magnetic bead separation technology and is used to solve the technical problem that the existing magnetic bead mother liquor preparation cannot be continuously operated and the preparation efficiency is low. It includes a base, a processing tube is fixed on the base, a stirring mechanism is arranged in the processing tube, a feeding tube and a derivation tube are respectively connected to the upper and lower ends of the processing tube, the derivation tube is connected to a feedback mechanism, a magnetic bead processing box is fixed on the base, and a magnetic bead separation mechanism, a magnetic bead cleaning mechanism and a magnetic bead feeding mechanism are arranged on the magnetic bead processing box; the present invention realizes the continuous production of magnetic bead mother liquor and improves the preparation efficiency of magnetic bead mother liquor; it can automatically separate and clean the magnetic beads so that the magnetic beads can be reused, or it can only separate the magnetic beads without reusing them to meet the high-precision preparation requirements; it automatically checks whether the prepared magnetic bead mother liquor is qualified to ensure the quality of the magnetic bead mother liquor, and at the same time it can provide feedback on the equipment status to facilitate the adjustment of equipment parameters.

Description

Technical Field

[0001] The invention belongs to the technical field of magnetic bead separation, and relates to a magnetic bead mother liquid preparation device, in particular to a high-efficiency and continuous magnetic bead mother liquid preparation device. Background Art

[0002] Magnetic beads are widely used in the in vitro diagnostic reagent industry. Usually, magnetic beads are coated to form magnetic bead master liquor. The preparation of magnetic bead master liquor is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. It completely solves the long-term dependence on imports for nucleic acid extraction and purification in my country.

[0003] At present, in the process of preparing magnetic bead mother liquor (i.e., coating process), the magnetic beads and the solution are fully mixed in a container, and then the magnetic beads and the mother liquor are separated to extract the mother liquor, and the extracted magnetic bead mother liquor is weighed. Each step usually requires manual operation, and can only be processed quantitatively. When the raw materials are single and repetitive, there is no need to worry about the differences of the products, the impurities are relatively small, and the production process is simple, the existing equipment cannot carry out continuous production, the degree of automation is low, and the preparation efficiency is low.

[0004] Based on this, we designed an efficient and continuous magnetic bead mother liquor preparation device. Summary of the invention

[0005] The purpose of the present invention is to address the above-mentioned problems in the existing technology and to propose a highly efficient and continuous magnetic bead mother liquor preparation device. The technical problem to be solved by the device is: how to achieve continuous production of magnetic bead mother liquor and improve the production efficiency of magnetic bead mother liquor.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A highly efficient and continuous magnetic bead mother liquor preparation device comprises a base, a first support frame is fixed on the base, a processing tube with sealed ends is fixed on the first support frame, the processing tube is tilted, a stirring mechanism is arranged inside the processing tube, and a feed tube and a lead-out tube are respectively connected to the upper and lower ends of the processing tube, a feed solenoid valve is installed on the feed tube, and a feedback mechanism is connected to the lead-out tube, a second support frame is fixed on the base, a magnetic bead processing box is fixed on the upper end of the second support frame, the magnetic bead processing box is located above the processing tube, and a magnetic bead separation mechanism, a magnetic bead cleaning mechanism and a magnetic bead feeding mechanism are arranged in sequence from top to bottom in the magnetic bead processing box, and a control box is fixed on the feedback mechanism.

[0008] The working principle of the present invention is: the operator transports the raw materials to the inside of the processing tube through the feed pipe, and at the same time, the magnetic bead feeding mechanism continuously injects magnetic beads into the processing tube, and then the stirring mechanism operates to stir the mixed material formed by the magnetic beads and the raw materials. With the continuous input of the raw materials and the magnetic beads, the liquid level of the mixed material in the processing tube gradually rises, and the mixed material goes from low to high, and the longer the stirring time is, when the mixed material moves to the upper end of the processing tube, the magnetic bead separation mechanism will take the magnetic beads in the solution out of the processing tube and move it to the magnetic bead processing box, after separating the magnetic beads in the mixed material, a magnetic bead mother liquor is obtained, and the magnetic bead mother liquor is pushed upward by the mixed material below, and finally flows out from the outlet tube, and the magnetic bead mother liquor flowing out of the outlet tube flows through the feedback mechanism, and when the magnetic bead mother liquor reaches the preparation standard, it flows out from the feedback mechanism, otherwise, the feed The solenoid valve is closed, and the magnetic bead mother liquor continues to enter the bottom of the processing tube and circulates until the composite standard is reached. At the same time, the magnetic beads entering the magnetic bead processing box continue to move downward into the magnetic bead cleaning mechanism. After sufficient cleaning, they move to the magnetic bead feeding mechanism to realize the recycling of the magnetic beads. When the feedback mechanism is running, it means that the stirring time of the mixed material is insufficient or the magnetic beads are reused too many times, and the adsorption effect is reduced. The operator can lower the magnetic bead input speed of the magnetic bead feeding mechanism and the raw material input speed of the feeding tube to reduce the rising speed of the mixed material and increase the stirring time of the mixed material in the stirring mechanism, so that the subsequent production of the magnetic bead mother liquor can meet the preparation standard, or a new batch of magnetic beads can be replaced to improve the adsorption effect of the magnetic beads, so that the subsequent production of the magnetic bead mother liquor can meet the preparation standard.

[0009] The stirring mechanism includes a rotating rod rotatably arranged in a processing tube, a stirring motor for driving the rotating rod is fixed at the end of the processing tube, a plurality of stirring components distributed at equal intervals are arranged on the rotating rod, the stirring component includes a first fixed ring fixed on the rotating rod and a plurality of stirring rods fixed on the outside of the first fixed ring and evenly distributed around the circumference, a plurality of fixed components distributed at equal intervals are fixed on the inner wall of the processing tube, the fixed component includes a second fixed ring fixed on the inner wall of the processing tube and a plurality of fixed rods fixed on the outside of the second fixed ring and evenly distributed around the circumference, and the stirring components and the fixed components are distributed alternately.

[0010] With the above structure, the stirring motor drives the rotating rod to rotate, the stirring component rotates, and the fixed component does not move, so that the mixed material is stirred more fully, and the fixed component does not move, and the stirring component can only stir in the same plane, which can reduce the up and down surge of the mixed material, prevent the mixed material below from surging upward and flowing out of the outlet pipe before the stirring time is reached, and prevent the quality of the prepared magnetic bead mother liquid from being unstable.

[0011] The feedback mechanism includes a turbidity meter connected to the outlet pipe, a three-way solenoid valve is connected below the turbidity meter, two outlets of the three-way solenoid valve are respectively connected to the outlet pipe and the recovery pipe, a water tank is fixed on the base, the upper end of the water tank is connected to the recovery pipe, a liquid level detector is installed on the side wall of the water tank, a recovery pump is fixed on the upper end of the water tank, the water inlet of the recovery pump is connected to the water tank through a hose, and the water outlet of the recovery pump is connected to the bottom of the processing pipe through another hose.

[0012] With the above structure, the magnetic bead mother liquor flowing out of the outlet tube passes through a turbidity meter and is detected by the turbidity meter. The magnetic bead mother liquor that meets the preparation standard flows to the discharge pipe after passing through a three-way solenoid valve. When the feed is too fast or the magnetic beads are reused too many times, the prepared magnetic bead mother liquor does not meet the preparation standard. At this time, the magnetic bead mother liquor flows to the recovery pipe after passing through the three-way solenoid valve, and finally flows into the water tank. When the liquid level detector in the water tank detects that the liquid level reaches the highest set value, the feed solenoid valve is closed, and the recovery pump pumps the magnetic bead mother liquor in the water tank into the bottom of the processing tube and stirs again until the liquid level in the water tank drops to the lowest set value. At this time, the recovery pump stops, the feed solenoid valve is opened, and the preparation of the magnetic bead mother liquor continues.

[0013] The magnetic bead separation mechanism includes a mounting frame fixed to the upper end of the inner part of the magnetic bead processing box, two first pulleys are rotatably arranged on the mounting frame, two second pulleys are fixed to the upper end of the rotating rod, two through holes are provided on the side wall of the processing tube, and two channels are provided on the lower surface of the magnetic bead processing box. Transmission belts are installed on the first pulley and the second pulley, and the transmission belts pass through the channels and the through holes. A soft magnetic strip is fixed between the two transmission belts, and a heightening cylinder and a scraping strip are fixed on the inner wall of the magnetic bead processing box. The heightening cylinder is sleeved on the outer side of the soft magnetic strip, and the upper end of the scraping strip is against the surface of the soft magnetic strip.

[0014] With the above structure, when the rotating rod rotates, the first pulley and the second pulley as well as the transmission belt and the soft magnetic strip all start to rotate. The soft magnetic strip adsorbs the magnetic beads in the mixed material in the processing tube on the surface, and then drives it to move upward into the magnetic bead processing box, and then is scraped off the soft magnetic strip by the scraper strip. The soft magnetic strip continues to enter the processing tube to adsorb the magnetic beads. Repeating this process can separate the magnetic beads in the mixed material from the magnetic bead mother liquor and bring the magnetic beads into the magnetic bead processing box. At this time, the heightening cylinder can prevent the magnetic column from contacting the soft magnetic strip again. When a certain amount of scraped magnetic beads accumulates, they will slide downward along the inner lower surface of the magnetic bead processing box and enter the magnetic bead cleaning mechanism.

[0015] The magnetic bead cleaning mechanism includes an isolation plate and a scraper fixed on the lower inner surface of the magnetic bead processing box, and an electromagnet is rotatably arranged between the isolation plate and the scraper, a protective sleeve is provided on the outer side of the electromagnet, and a transfer motor for driving the electromagnet to rotate is fixed on the outer side of the magnetic bead processing box, the two ends of the protective sleeve are abutted against the inner walls of the magnetic bead processing box on both sides, and the cylindrical side wall of the protective sleeve is abutted against the isolation plate and the scraper, a stirring roller is also rotatably arranged inside the magnetic bead processing box, the stirring roller is located above the isolation plate, and a cleaning motor for driving the stirring roller to rotate is fixed on the outer side of the magnetic bead processing box, a water inlet pipe and a water outlet pipe are respectively arranged on both sides of the magnetic bead processing box, and the connection positions of the water inlet pipe and the water outlet pipe with the magnetic bead processing box are both located above the isolation plate, and a water inlet solenoid valve and a water outlet solenoid valve are respectively arranged on the water inlet pipe and the water outlet pipe.

[0016] With the above structure, the water inlet pipe is connected to the external water supply equipment. When the separated magnetic beads need to be cleaned, the water inlet solenoid valve is opened, and the cleaning water enters the top of the isolation plate through the water inlet pipe. The magnetic beads that need to be cleaned also slide to the top of the isolation plate. At this time, the stirring roller rotates to clean the magnetic beads. After the cleaning is completed, the electromagnet is energized to adsorb the magnetic beads on the protective cover, and then the protective cover rotates to the side of the scraper. The magnetic beads are then scraped off by the scraper and slide into the magnetic bead feeding mechanism. Then the water outlet solenoid valve is opened, and the cleaning water is discharged through the water outlet pipe. At fixed intervals, the magnetic beads can be cleaned once after accumulating to a certain amount. When the magnetic beads do not need to be cleaned, the water inlet solenoid valve and the water outlet solenoid valve are opened at the same time, and the cleaning water continues to flow to bring the magnetic beads out of the water outlet pipe.

[0017] A rotating shaft is rotatably arranged in the magnetic bead processing box, and the rotating shaft is located above the stirring roller. An intermittent motor for driving the rotating shaft to rotate is fixed to the outside of the magnetic bead processing box, and a baffle is fixed below the rotating shaft, and the bottom and both sides of the baffle are respectively in contact with the inner wall of the magnetic bead processing box.

[0018] With the above structure, the intermittent motor starts at regular intervals, and the magnetic beads sliding down from above are blocked by the baffle. When a certain amount is accumulated, the baffle rotates upward, and the magnetic beads continue to move downward for cleaning. After the magnetic beads pass through the baffle, the baffle rotates downward to block the magnetic beads above again, ensuring that no magnetic beads continue to be added during the cleaning process, ensuring that the magnetic beads can be fully cleaned, and improving the cleaning effect.

[0019] The magnetic bead feeding mechanism includes a connecting channel connected to the bottom of the magnetic bead processing box, the end of the connecting channel is connected to a square shell, a magnetic bead inlet is opened at the bottom of the processing tube, the bottom of the square shell is inserted into the magnetic bead inlet, an L-shaped push plate is slidably arranged in the square shell, and a sealing strip is arranged around the bottom edge of the L-shaped push plate, an electric push rod is fixed to the upper end of the square shell, and the output end of the electric push rod is fixedly connected to the L-shaped push plate, an inspection port is opened at the side end of the square shell, and a magnetic bead input port is opened on the upper surface of the magnetic bead processing box.

[0020] With the above structure, the electric push rod continuously retracts and contracts. When the electric push rod contracts, the magnetic beads enter the square housing through the connecting channel. When the electric push rod extends, the L-shaped push plate pushes the magnetic beads downward into the processing tube. The inspection port is convenient for replacing or repairing the electric push rod, and the magnetic bead input port is convenient for adding a new batch of magnetic beads.

[0021] A sealing cover is provided at the lower end of the square shell, and a first protrusion is provided on both sides of the sealing cover, a round rod is fixed on the first protrusion, and a shoulder is provided at the end of the round rod, and a second protrusion is provided on both sides of the bottom of the square shell, and the second protrusion can be provided with a round hole, and the round rod passes through the round hole at the corresponding position, and a reset spring is sleeved on the round rod, and the two ends of the reset spring are respectively against the shoulder and the second protrusion.

[0022] With the above structure, when the L-shaped push plate moves upward, the sealing cover seals the bottom of the square shell under the action of the return spring to prevent the material in the processing tube from entering the square shell. When the L-shaped push plate moves downward, the magnetic beads push the sealing cover open and enter the processing tube from all sides.

[0023] Compared with the prior art, this efficient and continuous magnetic bead mother liquor preparation device has the following advantages:

[0024] 1. Through the processing tube, stirring mechanism and magnetic bead feeding mechanism, the continuous production of magnetic bead mother liquor is realized, the automation degree of magnetic bead mother liquor preparation is improved, and the preparation efficiency of magnetic bead mother liquor is improved.

[0025] 2. Through the magnetic bead separation mechanism and the magnetic bead cleaning mechanism, the magnetic beads can be automatically separated and cleaned, so that the magnetic beads can be reused, reducing the production cost. It is also possible to only separate the magnetic beads without reusing them, meeting the high-precision preparation requirements.

[0026] 3. Through the feedback mechanism, it can automatically check whether the prepared magnetic bead mother liquor is qualified, and circulate and stir the unqualified magnetic bead mother liquor to ensure the quality of the magnetic bead mother liquor. At the same time, it can provide feedback on the equipment status to facilitate the operator to adjust the equipment parameters. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a three-dimensional structural schematic diagram of the present invention;

[0028] Figure 2 It is a structural schematic diagram of the feedback mechanism in the present invention;

[0029] Figure 3 It is a schematic diagram of the internal structure of the processing tube in the present invention;

[0030] Figure 4 It is a partial structural schematic diagram of the stirring mechanism in the present invention;

[0031] Figure 5 It is a structural schematic diagram of the magnetic bead separation mechanism in the present invention;

[0032] Figure 6 It is a schematic diagram of the external structure of the magnetic bead cleaning mechanism of the present invention;

[0033] Figure 7 It is a schematic diagram of the internal structure of the magnetic bead cleaning mechanism of the present invention;

[0034] Figure 8 It is a structural schematic diagram of the magnetic bead feeding mechanism in the present invention;

[0035] Fig. 9 It is a schematic diagram of the internal structure of the square housing in the present invention;

[0036] In the figure: 1, base; 2, first support frame; 3, processing tube; 4, stirring mechanism; 401, rotating rod; 402, stirring motor; 403, stirring assembly; 404, fixed assembly; 5, feed pipe; 6, outlet pipe; 7, feedback mechanism; 701, turbidity meter; 702, three-way solenoid valve; 703, discharge pipe; 704, recovery pipe; 705, water tank; 706, liquid level detector; 707, recovery pump; 8, second support frame; 9, magnetic bead processing box; 10, magnetic bead separation mechanism; 1001, mounting frame; 1002, first pulley; 1003, second pulley; 1004, through hole; 1005, heightening cylinder; 1006, scraper strip; 1007, soft magnetic strip; 1008, transmission belt; 11, magnetic Bead cleaning mechanism; 1101, isolation plate; 1102, scraper; 1103, electromagnet; 1104, protective cover; 1105, transfer motor; 1106, stirring roller; 1107, cleaning motor; 1108, water inlet pipe; 1109, water outlet pipe; 1110, rotating shaft; 1111, intermittent motor; 1112, baffle; 12, magnetic bead feeding mechanism; 1201, magnetic bead input port; 1202, connecting channel; 1203, square shell; 1204, magnetic bead inlet; 1205, L-shaped push plate; 1206, electric push rod; 1207, inspection port; 1208, sealing cover; 1209, first protrusion; 1210, second protrusion; 1211, round rod; 1212, reset spring; 13, control box. DETAILED DESCRIPTION

[0037] The technical solution of this patent is further described in detail below in conjunction with specific implementation methods.

[0038] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0039] In the description of this patent, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this patent.

[0040] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, it can be fixedly connected or set, or it can be detachably connected or set, or connected or set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0041] See also Figure 1-9The present embodiment provides a highly efficient and continuous magnetic bead mother liquid preparation device, comprising a base 1, a first support frame 2 is fixed on the base 1, a processing tube 3 with sealed ends is fixed on the first support frame 2, the processing tube 3 is tilted, a stirring mechanism 4 is arranged inside the processing tube 3, and a feed tube 5 and a lead-out tube 6 are connected to the upper and lower ends of the processing tube 3 respectively, a feed solenoid valve is installed on the feed tube 5, and a feedback mechanism 7 is connected to the lead-out tube 6, a second support frame 8 is fixed on the base 1, a magnetic bead processing box 9 is fixed on the upper end of the second support frame 8, the magnetic bead processing box 9 is located above the processing tube 3, and the magnetic bead processing box 9 is connected from above A magnetic bead separation mechanism 10, a magnetic bead cleaning mechanism 11 and a magnetic bead feeding mechanism 12 are sequentially arranged below, and a control box 13 is fixed on the feedback mechanism 7; the operator transports the raw material into the processing tube 3 through the feeding tube 5, and at the same time, the magnetic bead feeding mechanism 12 continuously injects magnetic beads into the processing tube 3, and then the stirring mechanism 4 is operated to stir the mixed material formed by the magnetic beads and the raw material. With the continuous input of the raw material and the magnetic beads, the liquid level of the mixed material in the processing tube 3 gradually rises, and the mixed material goes from low to high. The longer the stirring time, when the mixed material moves to the upper end of the processing tube 3, the magnetic bead separation mechanism 10 will The magnetic beads in the solution are taken out of the processing tube 3 and moved to the magnetic bead processing box 9. After the magnetic beads in the mixed material are separated, a magnetic bead mother liquor is obtained. The magnetic bead mother liquor is pushed upward by the mixed material below and finally flows out from the outlet tube 6. The magnetic bead mother liquor flowing out of the outlet tube 6 flows through the feedback mechanism 7. When the magnetic bead mother liquor reaches the preparation standard, it flows out of the feedback mechanism 7. Otherwise, the feed solenoid valve is closed, and the magnetic bead mother liquor continues to enter the bottom of the processing tube 3 and circulates until the composite standard is reached. At the same time, the magnetic beads entering the magnetic bead processing box 9 continue to move downward and enter the magnetic bead cleaning mechanism 11. After sufficient cleaning, Move to the magnetic bead feeding mechanism 12 to realize the recycling of magnetic beads. When the feedback mechanism 7 is running, it means that the stirring time of the mixed material is insufficient or the magnetic beads are reused too many times, and the adsorption effect is reduced. The operator can lower the magnetic bead input speed of the magnetic bead feeding mechanism 12 and the raw material input speed of the feeding tube 5 to reduce the rising speed of the mixed material and increase the stirring time of the mixed material in the stirring mechanism 4, so that the subsequent production of magnetic bead mother liquor can meet the preparation standard, or a new batch of magnetic beads can be replaced to improve the adsorption effect of the magnetic beads, so that the subsequent production of magnetic bead mother liquor can meet the preparation standard.

[0042] The stirring mechanism 4 includes a rotating rod 401 rotatably arranged in the processing tube 3, a stirring motor 402 for driving the rotating rod 401 is fixed at the end of the processing tube 3, a plurality of stirring components 403 distributed at equal intervals are arranged on the rotating rod 401, the stirring components 403 include a first fixed ring fixed on the rotating rod 401 and a plurality of stirring rods fixed outside the first fixed ring and evenly distributed around the circumference, a plurality of fixed components 404 distributed at equal intervals are fixed on the inner wall of the processing tube 3, the fixed components 404 include a second fixed ring fixed on the inner wall of the processing tube 3 and a plurality of A plurality of fixed rods are fixed on the outside of the second fixed ring and are evenly distributed around the circumference, and the stirring components 403 and the fixed components 404 are distributed alternately; the stirring motor 402 drives the rotating rod 401 to rotate, the stirring component 403 rotates, and the fixed component 404 does not move, so that the mixed material is stirred more fully, and the fixed component 404 does not move, and the stirring component 403 can only stir in the same plane, which can reduce the up and down surge of the mixed material, prevent the mixed material below from surging upward and flowing out of the outlet pipe 6 before the stirring time is reached, and prevent the quality of the prepared magnetic bead mother liquid from being unstable.

[0043] The feedback mechanism 7 includes a turbidity meter 701 connected to the outlet pipe 6, a three-way solenoid valve 702 is connected to the lower part of the turbidity meter 701, and two outlets of the three-way solenoid valve 702 are respectively connected to the outlet pipe 703 and the recovery pipe 704, a water tank 705 is fixed on the base 1, the upper end of the water tank 705 is connected to the recovery pipe 704, a liquid level detector 706 is installed on the side wall of the water tank 705, and a recovery pump 707 is fixed to the upper end of the water tank 705, and the water inlet of the recovery pump 707 is connected to the water tank 705. The box 705 is connected by a hose, and the outlet of the recovery pump 707 is connected to the bottom of the processing tube 3 by another hose; the magnetic bead mother liquor flowing out of the outlet pipe 6 passes through the turbidity meter 701, and is detected by the turbidity meter 701. The magnetic bead mother liquor that meets the preparation standard flows to the discharge pipe 703 after passing through the three-way solenoid valve 702. When the feed is too fast or the magnetic beads are reused too many times, the prepared magnetic bead mother liquor does not meet the preparation standard. At this time, the magnetic bead mother liquor flows to the recovery pipe after passing through the three-way solenoid valve 702 704, and finally flows into the water tank 705. When the liquid level detector 706 in the water tank 705 detects that the liquid level has reached the highest set value, the feed solenoid valve is closed, and the recovery pump 707 pumps the magnetic bead mother liquor in the water tank 705 into the bottom of the processing tube 3, and stirs again until the liquid level in the water tank 705 drops to the lowest set value. At this time, the recovery pump 707 stops, the feed solenoid valve is opened, and the preparation of the magnetic bead mother liquor continues. When the feedback mechanism 7 is running, it means that the stirring time of the mixed material is insufficient or the magnetic beads are reused too many times, and the adsorption effect is reduced. The operator can lower the magnetic bead input speed of the magnetic bead feeding mechanism 12 and the raw material input speed of the feeding tube 5, so that the rising speed of the mixed material is reduced, and the stirring time of the mixed material in the stirring mechanism 4 is increased, so that the magnetic bead mother liquor produced subsequently can meet the preparation standard, or a new batch of magnetic beads can be replaced to improve the adsorption effect of the magnetic beads, so that the magnetic bead mother liquor produced subsequently can meet the preparation standard.

[0044] The magnetic bead separation mechanism 10 includes a mounting frame 1001 fixed to the upper end of the inner part of the magnetic bead processing box 9, two first pulleys 1002 are rotatably provided on the mounting frame 1001, two second pulleys 1003 are fixed to the upper end of the rotating rod 401, two through holes 1004 are provided on the side wall of the processing tube 3, and two channels are provided on the lower surface of the magnetic bead processing box 9, a transmission belt 1008 is installed on the first pulley 1002 and the second pulley 1003, the transmission belt 1008 passes through the channel and the through hole 1004, a soft magnetic strip 1007 is fixed between the two transmission belts 1008, a heightening cylinder 1005 and a scraping strip 1006 are fixed on the inner wall of the magnetic bead processing box 9, the heightening cylinder 1005 is sleeved on the outer side of the soft magnetic strip 1007, and the upper end of the scraping strip 1006 is connected to the soft magnetic strip 1007 The surfaces are against each other; when the rotating rod 401 rotates, the first pulley 1002 and the second pulley 1003, the transmission belt 1008 and the soft magnetic strip 1007 all start to rotate, and the soft magnetic strip 1007 adsorbs the magnetic beads in the mixed material in the processing tube 3 on the surface, and then drives it to move upward into the magnetic bead processing box 9, and then is scraped off from the soft magnetic strip 1007 by the scraper strip 1006, and the soft magnetic strip 1007 continues to enter the processing tube 3 to adsorb the magnetic beads. Repeating this process can separate the magnetic beads in the mixed material from the magnetic bead mother liquor and bring the magnetic beads into the magnetic bead processing box 9. At this time, the heightening cylinder 1005 can prevent the magnetic column from contacting the soft magnetic strip 1007 again. After a certain amount of the scraped magnetic beads accumulate, they will slide downward along the inner lower surface of the magnetic bead processing box 9 and enter the magnetic bead cleaning mechanism 11.

[0045] The magnetic bead cleaning mechanism 11 includes an isolation plate 1101 and a scraper 1102 fixed to the lower surface of the inner side of the magnetic bead processing box 9, and an electromagnet 1103 is rotatably arranged between the isolation plate 1101 and the scraper 1102, and a protective sleeve 1104 is sleeved on the outer side of the electromagnet 1103, and a transfer motor 1105 for driving the electromagnet 1103 to rotate is fixed on the outer side of the magnetic bead processing box 9, and the two ends of the protective sleeve 1104 are against the inner walls of the two sides of the magnetic bead processing box 9, and the cylindrical side wall of the protective sleeve 1104 is The isolation plate 1101 and the scraper 1102 are against each other, and a stirring roller 1106 is also rotatably arranged inside the magnetic bead processing box 9, and the stirring roller 1106 is located above the isolation plate 1101, and a cleaning motor 1107 for driving the stirring roller 1106 to rotate is fixed to the outside of the magnetic bead processing box 9, and a water inlet pipe 1108 and a water outlet pipe 1109 are respectively arranged on both sides of the magnetic bead processing box 9, and the connection positions of the water inlet pipe 1108 and the water outlet pipe 1109 with the magnetic bead processing box 9 are both located above the isolation plate 1101. , and the water inlet pipe 1108 and the water outlet pipe 1109 are respectively provided with a water inlet solenoid valve and a water outlet solenoid valve; the water inlet pipe 1108 is connected to the external water supply equipment. When the separated magnetic beads need to be cleaned, the water inlet solenoid valve is opened, and the cleaning water enters the isolation plate 1101 through the water inlet pipe 1108. The magnetic beads to be cleaned also slide to the top of the isolation plate 1101. At this time, the stirring roller 1106 rotates to clean the magnetic beads. After the cleaning is completed, the electromagnet 1103 is energized to absorb the magnetic beads. Attached to the protective cover 1104, and then rotated with the protective cover 1104 to the side of the scraper 1102, the magnetic beads are then scraped off by the scraper 1102 and slide into the magnetic bead feeding mechanism 12, and then the water outlet solenoid valve is opened, and the cleaning water is discharged through the water outlet pipe 1109. At fixed intervals, the magnetic beads can be cleaned once after accumulating to a certain amount. When the magnetic beads do not need to be cleaned, the water inlet solenoid valve and the water outlet solenoid valve are opened at the same time, and the cleaning water continues to flow to bring the magnetic beads out of the water outlet pipe 1109.

[0046] A rotating shaft 1110 is rotatably provided in the magnetic bead processing box 9, and the rotating shaft 1110 is located above the stirring roller 1106. An intermittent motor 1111 is fixed on the outside of the magnetic bead processing box 9 for driving the rotating shaft 1110 to rotate, and a baffle 1112 is fixed below the rotating shaft 1110, and the bottom and both sides of the baffle 1112 are respectively in contact with the inner wall of the magnetic bead processing box 9; the intermittent motor 1111 is started once at regular intervals, and the magnetic beads sliding down from the top are blocked by the baffle 1112. When a certain amount is accumulated, the baffle 1112 rotates upward, and the magnetic beads continue to move downward for cleaning. After the magnetic beads pass through the baffle 1112, the baffle 1112 rotates downward to block the magnetic beads above again, ensuring that no magnetic beads are continuously added during the cleaning process, ensuring that the magnetic beads can be fully cleaned, and improving the cleaning effect.

[0047] The magnetic bead feeding mechanism 12 includes a connecting channel 1202 connected to the bottom of the magnetic bead processing box 9, the end of the connecting channel 1202 is connected to a square shell 1203, a magnetic bead inlet 1204 is provided at the bottom of the processing tube 3, the bottom of the square shell 1203 is inserted into the magnetic bead inlet 1204, an L-shaped push plate 1205 is slidably arranged in the square shell 1203, and a sealing strip is arranged around the bottom edge of the L-shaped push plate 1205, an electric push rod 1206 is fixed to the upper end of the square shell 1203, and the output end of the electric push rod 1206 is connected to the L-shaped push plate 1 205 is fixedly connected, an inspection port 1207 is provided at the side end of the square shell 1203, and a magnetic bead input port 1201 is provided on the upper surface of the magnetic bead processing box 9; the electric push rod 1206 continuously retracts and contracts, and when the electric push rod 1206 contracts, the magnetic beads enter the square shell 1203 through the connecting channel 1202, and when the electric push rod 1206 extends, the L-shaped push plate 1205 pushes the magnetic beads downward into the processing tube 3, the inspection port 1207 is convenient for replacing or repairing the electric push rod 1206, and the magnetic bead input port 1201 is convenient for adding a new batch of magnetic beads.

[0048] A sealing cover 1208 is provided at the lower end of the square shell 1203, and first protrusions 1209 are provided on both sides of the sealing cover 1208. A round rod 1211 is fixed on the first protrusion 1209, and a shoulder is provided at the end of the round rod 1211. Second protrusions 1210 are provided on both sides of the bottom of the square shell 1203, and the second protrusions 1210 can be provided with round holes, and the round rod 1211 passes through the round holes at corresponding positions, and a return spring 1212 is sleeved on the round rod 1211, and the two ends of the return spring 1212 are respectively against the shoulder and the second protrusion 1210; when the L-shaped push plate 1205 moves upward, the sealing cover 1208 seals the bottom of the square shell 1203 under the action of the return spring 1212 to prevent the material in the processing tube 3 from entering the square shell 1203, and when the L-shaped push plate 1205 moves downward, the magnetic beads will push the sealing cover 1208 open and enter the processing tube 3 from all sides.

[0049] The above-mentioned fixing methods are the most commonly used fixing connection methods in this field, such as welding, bolt connection, interference fit, key connection, bonding, etc.; the above-mentioned electrical components, such as the feed solenoid valve, stirring motor 402, turbidity meter 701, three-way solenoid valve 702, liquid level detector 706, electromagnet 1103, transfer motor 1105, cleaning motor 1107, water inlet solenoid valve, water outlet solenoid valve, intermittent motor 1111 and electric push rod 1206, etc., are all existing technology products and can be directly purchased and used in the market, and the specific principles will not be repeated.

[0050] Working principle of the present invention:

[0051] The operator transports the raw materials into the processing tube 3 through the feed pipe 5. At the same time, the magnetic bead feeding mechanism 12 continuously injects magnetic beads into the processing tube 3, and the electric push rod 1206 continuously retracts and contracts. When the electric push rod 1206 contracts, the magnetic beads enter the square shell 1203 through the connecting channel 1202. When the electric push rod 1206 extends, the L-shaped push plate 1205 pushes the magnetic beads downward into the processing tube 3. The inspection port 1207 is convenient for replacing or repairing the electric push rod 1206, and the magnetic bead input port 1201 is convenient for adding a new batch of magnetic beads. When the L-shaped push plate 1205 moves upward, the sealing cover 1208 seals the bottom of the square shell 1203 under the action of the reset spring 1212 to prevent the material in the processing tube 3 from entering the square shell 1203. When the L-shaped push plate 1205 moves downward, the magnetic beads will push the sealing cover 1208 open and enter the processing tube 3 from all sides, and then the stirring motor 402 drives the rotating rod 401 to rotate, the stirring component 403 rotates, and the fixed component 404 does not move, so that the mixed material is stirred more fully, and the fixed component 404 does not move, and the stirring component 403 can only stir on the same plane, which can reduce the up and down surge of the mixed material, and prevent the mixed material below from surging upward and flowing out of the outlet pipe 6 before the stirring time is reached, so as to prevent the quality of the prepared magnetic bead mother liquor from being unstable. With the continuous input of raw materials and magnetic beads, the liquid level of the mixed material in the processing tube 3 gradually rises, and the mixed material goes from low to high. The longer the stirring time, when the mixed material moves to the processing tube 3 When the magnetic beads are at the upper end, the magnetic bead separation mechanism 10 will take the magnetic beads in the solution out of the processing tube 3 and move it to the magnetic bead processing box 9. After separating the magnetic beads in the mixed material, a magnetic bead mother liquor is obtained. The magnetic bead mother liquor is pushed upward by the mixed material below and finally flows out from the outlet tube 6. The magnetic bead mother liquor flowing out of the outlet tube 6 passes through the turbidity meter 701 and is detected by the turbidity meter 701. The magnetic bead mother liquor that meets the preparation standard flows to the discharge pipe 703 after passing through the three-way solenoid valve 702. When the feed is too fast or the magnetic beads are reused too many times, the prepared magnetic bead mother liquor does not meet the preparation standard. At this time, the magnetic bead mother liquor flows to the recovery pipe 704 after passing through the three-way solenoid valve 702, and finally flows into the water tank 705. When the liquid level detector 706 in the water tank 705 detects that the liquid level has reached After reaching the highest set value, the feed solenoid valve is closed, and the recovery pump 707 pumps the magnetic bead mother liquor in the water tank 705 into the bottom of the processing tube 3, and stirs again until the liquid level in the water tank 705 drops to the lowest set value. At this time, the recovery pump 707 stops, the feed solenoid valve is opened, and the preparation of the magnetic bead mother liquor continues. At the same time, when the rotating rod 401 rotates, the first pulley 1002 and the second pulley 1003, the transmission belt 1008 and the soft magnetic strip 1007 all start to rotate. The soft magnetic strip 1007 adsorbs the magnetic beads in the mixed material in the processing tube 3 on the surface, and then drives it to move upward into the magnetic bead processing box 9, and then is scraped off from the soft magnetic strip 1007 by the scraper strip 1006. The soft magnetic strip 1007 continues to enter the processing tube 3 to adsorb the magnetic beads.Repeating the process can separate the magnetic beads from the magnetic bead mother liquor in the mixed material and bring the magnetic beads into the magnetic bead processing box 9. At this time, the heightening cylinder 1005 can prevent the magnetic column from contacting the soft magnetic strip 1007 again. When a certain amount of scraped magnetic beads accumulate, they will slide downward along the inner lower surface of the magnetic bead processing box 9, and the water inlet solenoid valve will be opened. The cleaning water will enter the top of the isolation plate 1101 through the water inlet pipe 1108, and the magnetic beads to be cleaned will also slide to the top of the isolation plate 1101. At this time, the stirring roller 1106 can rotate to clean the magnetic beads. After cleaning, the electromagnet 1103 is energized to adsorb the magnetic beads on the protective cover 1104, and then the protective cover 1104 rotates to the side of the scraper 1102. The magnetic beads are then scraped off by the scraper 1102 and slide into the magnetic bead feeding mechanism 12 for recycling. In addition, the intermittent motor 1111 is started once at a certain interval, and the magnetic beads sliding down from the top are blocked by the baffle 111 2 blocking, when a certain amount is accumulated, the baffle 1112 rotates upward, and the magnetic beads continue to move downward for cleaning. After the magnetic beads pass through the baffle 1112, the baffle 1112 rotates downward to block the magnetic beads above again, ensuring that no magnetic beads are continuously added during the cleaning process, ensuring that the magnetic beads can be fully cleaned, improving the cleaning effect, and realizing the recycling of magnetic beads. Moreover, when the feedback mechanism 7 is running, it means that the stirring time of the mixed material is not enough or the magnetic beads are reused too many times, and the adsorption effect is reduced. The operator can lower the magnetic bead input speed of the magnetic bead feeding mechanism 12 and the raw material input speed of the feeding pipe 5, so that the rising speed of the mixed material is reduced, and the stirring time of the mixed material in the stirring mechanism 4 is increased, so that the subsequent production of magnetic bead mother liquor can meet the preparation standard, or a new batch of magnetic beads can be replaced to improve the adsorption effect of the magnetic beads, so that the subsequent production of magnetic bead mother liquor can meet the preparation standard. ,

[0052] The above describes in detail the preferred implementation of this patent, but this patent is not limited to the above implementation. Various changes can be made within the knowledge scope of ordinary technicians in this field without departing from the purpose of this patent.

Claims

1. A highly efficient and continuous magnetic bead mother solution preparation device, comprising a base (1), characterized in that: A first support frame (2) is fixed on the base (1), a processing tube (3) with sealed ends is fixed on the first support frame (2), the processing tube (3) is tilted, a stirring mechanism (4) is provided inside the processing tube (3), the upper end of the processing tube (3) is connected to a discharge tube (6), the lower end of the processing tube (3) is connected to a feed tube (5), a feed solenoid valve is installed on the feed tube (5), and a feedback mechanism (7) is connected to the discharge tube (6), a second support frame (8) is fixed on the base (1), a magnetic bead processing box (9) is fixed on the upper end of the second support frame (8), the magnetic bead processing box (9) is located above the processing tube (3), and the magnetic bead processing box (9) is provided with a magnetic bead separation mechanism (10), a magnetic bead cleaning mechanism (11) and a magnetic bead feeding mechanism (12) in sequence from top to bottom, and a control box (13) is fixed on the feedback mechanism (7); The feedback mechanism (7) comprises a turbidity meter (701) connected to the outlet pipe (6); a three-way solenoid valve (702) is connected below the turbidity meter (701); two discharge ports of the three-way solenoid valve (702) are respectively connected to the discharge pipe (703) and the recovery pipe (704); a water tank (705) is fixed on the base (1); the upper end of the water tank (705) is connected to the recovery pipe (704); a liquid level detector (706) is installed on the side wall of the water tank (705); a recovery pump (707) is fixed on the upper end of the water tank (705); the water inlet of the recovery pump (707) is connected to the water tank (705) via a hose; and the water outlet of the recovery pump (707) is connected to the bottom of the processing pipe (3) via another hose; The magnetic bead separation mechanism (10) comprises a mounting frame (1001) fixed to the upper end of the inner part of the magnetic bead processing box (9), two first pulleys (1002) are rotatably arranged on the mounting frame (1001), two second pulleys (1003) are fixed to the upper end of the rotating rod (401), two through holes (1004) are provided on the side wall of the processing tube (3), two channels are provided on the lower surface of the magnetic bead processing box (9), and the first pulley (1002) and the second pulley (1003) are provided on the upper end of the rotating rod (401). A transmission belt (1008) is installed on the wheel (1003), and the transmission belt (1008) passes through the channel and the through hole (1004). A soft magnetic strip (1007) is fixed between the two transmission belts (1008). A heightening cylinder (1005) and a scraping strip (1006) are fixed on the inner wall of the magnetic bead processing box (9). The heightening cylinder (1005) is sleeved on the outside of the soft magnetic strip (1007), and the upper end of the scraping strip (1006) is against the surface of the soft magnetic strip (1007).

2. A highly efficient and continuous magnetic bead mother solution preparation device according to claim 1, characterized in that: The stirring mechanism (4) comprises a rotating rod (401) rotatably arranged in the processing tube (3); a stirring motor (402) for driving the rotating rod (401) is fixed at the end of the processing tube (3); a plurality of stirring components (403) distributed at equal intervals are arranged on the rotating rod (401); the stirring components (403) comprise a first fixing ring fixed on the rotating rod (401) and a plurality of stirring rods fixed on the outside of the first fixing ring and evenly distributed around the circumference; a plurality of fixing components (404) distributed at equal intervals are fixed on the inner wall of the processing tube (3); the fixing components (404) comprise a second fixing ring fixed on the inner wall of the processing tube (3) and a plurality of fixing rods fixed on the outside of the second fixing ring and evenly distributed around the circumference; the stirring components (403) and the fixing components (404) are distributed alternately.

3. The high-efficiency and continuous magnetic bead mother solution preparation device according to claim 1, characterized in that: The magnetic bead cleaning mechanism (11) comprises an isolation plate (1101) and a scraper (1102) fixed to the inner lower surface of a magnetic bead processing box (9), an electromagnet (1103) is rotatably arranged between the isolation plate (1101) and the scraper (1102), a protective sleeve (1104) is sleeved on the outer side of the electromagnet (1103), and a transfer motor (1105) for driving the electromagnet (1103) to rotate is fixed on the outer side of the magnetic bead processing box (9), two ends of the protective sleeve (1104) are against the inner walls of the magnetic bead processing box (9), and the cylindrical side wall of the protective sleeve (1104) is in contact with the isolation plate (1101) and the scraper (1102). A stirring roller (1106) is also rotatably arranged inside the magnetic bead processing box (9), the stirring roller (1106) is located above the isolation plate (1101), and a cleaning motor (1107) for driving the stirring roller (1106) to rotate is fixed to the outside of the magnetic bead processing box (9), a water inlet pipe (1108) and a water outlet pipe (1109) are respectively arranged on both sides of the magnetic bead processing box (9), and the connection positions of the water inlet pipe (1108) and the water outlet pipe (1109) with the magnetic bead processing box (9) are both located above the isolation plate (1101), and a water inlet solenoid valve and a water outlet solenoid valve are respectively arranged on the water inlet pipe (1108) and the water outlet pipe (1109).

4. A highly efficient and continuous magnetic bead mother solution preparation device according to claim 3, characterized in that: A rotating shaft (1110) is rotatably arranged in the magnetic bead processing box (9), the rotating shaft (1110) being located above the stirring roller (1106), an intermittent motor (1111) for driving the rotating shaft (1110) to rotate is fixed to the outside of the magnetic bead processing box (9), a baffle (1112) is fixed below the rotating shaft (1110), and the bottom and both sides of the baffle (1112) are respectively in contact with the inner wall of the magnetic bead processing box (9).

5. The high-efficiency and continuous magnetic bead mother solution preparation device according to claim 1, characterized in that: The magnetic bead feeding mechanism (12) comprises a connecting channel (1202) connected to the bottom of the magnetic bead processing box (9); the end of the connecting channel (1202) is connected to a square housing (1203); the bottom of the processing tube (3) is provided with a magnetic bead inlet (1204); the bottom of the square housing (1203) is inserted into the magnetic bead inlet (1204); an L-shaped push plate (1205) is slidably arranged in the square housing (1203); sealing strips are arranged around the bottom edge of the L-shaped push plate (1205); an electric push rod (1206) is fixed to the upper end of the square housing (1203); the output end of the electric push rod (1206) is fixedly connected to the L-shaped push plate (1205); a maintenance port (1207) is provided at the side end of the square housing (1203); and a magnetic bead input port (1201) is provided on the upper surface of the magnetic bead processing box (9).

6. The high-efficiency and continuous magnetic bead mother solution preparation device according to claim 5, characterized in that: A sealing cover (1208) is provided at the lower end of the square housing (1203), and first protrusions (1209) are provided on both sides of the sealing cover (1208). A round rod (1211) is fixed on the first protrusion (1209), and a shoulder is provided at the end of the round rod (1211). Second protrusions (1210) are provided on both sides of the bottom of the square housing (1203), and the second protrusions (1210) can be provided with round holes, and the round rod (1211) passes through the round holes at corresponding positions, and a return spring (1212) is sleeved on the round rod (1211), and the two ends of the return spring (1212) respectively abut against the shoulder and the second protrusion (1210).

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