A device for depilation of experimental mice for vitiligo research

Abstract

The application belongs to the technical field of vitiligo animal models, and particularly relates to a hair removal device for experimental mice used for vitiligo research. The hair removal device comprises a base and a dosing device. The base is provided with a rack and a support. The support is provided with a fixing clamp. The base is provided with a bearing seat. The bearing seat is provided with a bearing groove. The dosing device comprises a base ring, a movable ring and a syringe barrel. The movable ring is rotatably installed on the base ring. The base ring comprises a first ring body. The first ring body is provided with a first mounting seat. The first mounting seat is provided with a first gear and a second gear. The first gear is provided with a transmission groove. The second gear is connected with a transmission wheel. The first gear and the transmission groove are provided with a transmission rope. The movable ring comprises a second ring body. The second ring body is provided with a gear ring. The second ring body is provided with a driving ring. The problems that the mouse tail is thin and inconvenient to operate when manually applying, it is difficult to evenly apply the hair removal cream on the surface of the mouse tail without wasting the hair removal cream, and the hair removal is troublesome are solved.

Description

Technical Field

[0001] This invention belongs to the field of animal model technology for vitiligo, and specifically relates to a hair removal device for experimental mice used in vitiligo research. Background Technology

[0002] Previous studies have found that melanoma cells and melanocytes share common antigens, and immunotherapy for melanoma can activate CD8+ T cells that recognize these common antigens, leading to vitiligo in a portion (13.5%-25.7%) of melanoma patients receiving immunotherapy. Furthermore, Treg cells can induce CD8+ T cell apoptosis, and the absence of Treg cells can induce vitiligo. Based on these principles, in establishing an animal model, B16F10 melanoma cells were inoculated and supplemented with Treg cell depletion to activate endogenous autoreactive CD8+ T cells in C57BL / 6 mice, which then targeted and attacked epidermal melanocytes, ultimately inducing vitiligo in mice.

[0003] Because the melanocytes on the back of mice are located in the hair follicles rather than the epidermis; only the skin on their tails is similar to that of humans, where melanocytes are present in both the hair follicles and the epidermis. Since the absence of epidermal melanocytes is an important pathological feature of vitiligo, we chose to use mouse tail skin for subsequent depigmentation analysis of vitiligo.

[0004] After treating the mice, they need to be stained with fluorescent dye before analysis. During staining, the mouse tail needs to be cut off and the hair removed. Currently, hair removal is mostly done by hand. However, because the mouse tail is thin, it is difficult to operate by hand and it is hard to evenly apply the hair removal cream to the surface of the tail without wasting it, making hair removal quite troublesome. Summary of the Invention

[0005] Based on the problems mentioned in the background art above, the present invention provides a hair removal device for experimental mice used in vitiligo research, which solves the problem that most hair removal is done manually, which is inconvenient to operate when the mouse tail is thin, and it is difficult to evenly apply the hair removal cream to the surface of the mouse tail without wasting the cream, making hair removal quite troublesome.

[0006] The technical solution adopted in this invention is as follows:

[0007] A hair removal device for laboratory mice used in vitiligo research includes a base and a drug delivery device. The base has a rack and a support, and a fixing clamp is mounted on the support. A support seat is slidably mounted on the base, and the support seat has a groove. The drug delivery device includes a base ring, a movable ring, and an injection cylinder. The movable ring is rotatably mounted on the base ring. The base ring includes a first ring body, on which a first mounting seat and a traction frame are mounted. The base ring is moved by pulling the traction frame. A first gear and a second gear are mounted on the first mounting seat. The first gear has a transmission groove, and the second gear is coaxially connected to a transmission wheel. A transmission rope is wound between the first gear and the transmission groove. The movable ring includes a second ring body, rotatably mounted on the first ring body. The second ring has a gear ring that meshes with the second gear. When the first gear rotates, it drives the second gear to rotate via the transmission rope, thereby driving the device. The second ring rotates, and a drive ring with a transmission screw on its end face is installed on the second ring. The first ring has an axially open through hole, and a first sleeve is fixedly installed in the through hole. A first drug delivery cartridge is slidably installed in the through hole. A hose is connected between the first sleeve and the first drug delivery cartridge. The hose is used to deliver hair removal cream and also to prevent the first drug delivery cartridge from leaving the through hole. At the same time, the first drug delivery cartridge will not cause noise to the hose when it moves and squeezes it. The first drug delivery cartridge is movable and can be used for rat tails of different thicknesses. The injection cylinder includes an outer cylinder, a piston, and a connecting rod. A second drug delivery cartridge is provided on the outer cylinder. The second drug delivery cartridge passes through the first sleeve. A movable claw and a positioning block are installed on the connecting rod. The movable claw has a toothed groove that matches the transmission screw on the drive ring. The drive ring is connected to the movable claw in a transmission manner. The positioning block is axially fitted with the outer cylinder. A flexible coating is installed on the second ring.

[0008] Based on the above technical solution, the present invention has also made the following improvements:

[0009] Furthermore, the base ring is provided with a hexagonal cylinder corresponding to the through hole, and the outer cylinder is provided with a positioning sleeve that matches the hexagonal cylinder. The hexagonal cylinder and the positioning sleeve can be used to align the injection cylinder, so that the movable claw matches the second ring body, and at the same time, the injection cylinder is more stable after installation to prevent shaking during use.

[0010] Furthermore, the base is equipped with a slide rail, and the first mounting base is equipped with a sliding bracket that matches the slide rail. The sliding bracket is slidably engaged with the slide rail. The sliding bracket stabilizes the applicator, eliminating the need for manual stabilization when applying hair removal cream, making it convenient to use.

[0011] Furthermore, the second gear is mounted on the first mounting base via a rotating shaft, and a ratchet mechanism is installed between the transmission wheel and the rotating shaft. Under the action of the ratchet mechanism, the transmission wheel can only perform unidirectional transmission to the rotating shaft, thereby preventing the medication dispenser from driving the movable ring to rotate and causing the movable pawl to retract when it retracts along the rack, which would result in the piston retracting and affect subsequent use.

[0012] Furthermore, the first ring body is provided with two hexagonal cylinders, both located in the upper half of the first ring body, with an included angle between the two hexagonal cylinders ranging from 70° to 90°. Each hexagonal cylinder is equipped with an injection tube. This increases the dosage, and the squeezed-out hair removal cream, located in the upper half of the rat's tail, is less likely to fall off, facilitating subsequent application with a flexible smear.

[0013] Furthermore, a timer is installed on the base. After applying the hair removal cream to the rat's tail, the timer allows for better control of the hair removal time.

[0014] Furthermore, a rubber pad is fixedly installed on the bottom of the base. The rubber pad increases the frictional resistance at the bottom of the base, preventing the base from sliding during use.

[0015] The beneficial effects of this invention are:

[0016] Place the rat's tail on the receiving groove and pass one end of the tail through the applicator and clamp it to the fixed clamp. Under the action of the rack, the moving base ring can drive the first gear to rotate. The first gear drives the transmission wheel to rotate through the transmission rope, which in turn drives the second gear to rotate, causing the second ring to rotate. The rotating second ring drives the movable claw to move and squeeze out the hair removal cream from the outer cylinder. At the same time, the flexible coating plate installed on the second ring rotates and moves to evenly apply the squeezed hair removal cream to the surface of the rat's tail. After a certain period of time, use a tissue to wipe away the hair removal cream to complete the hair removal. It is convenient to use, simple to operate, has high application efficiency, and the application is relatively even. Attached Figure Description

[0017] The present invention can be further illustrated by the non-limiting embodiments given in the accompanying drawings;

[0018] Figure 1 This is a schematic diagram of a hair removal device for experimental mice used in vitiligo research according to an embodiment of the present invention;

[0019] Figure 2 This is a partial structural schematic diagram of a hair removal device for experimental mice used in vitiligo research according to an embodiment of the present invention;

[0020] Figure 3 This is a schematic diagram of the drug delivery device in an embodiment of the present invention;

[0021] Figure 4 This is a schematic diagram of the disassembled structure of the drug delivery device in an embodiment of the present invention;

[0022] Figure 5 This is a partial cross-sectional schematic diagram of the drug delivery device in an embodiment of the present invention;

[0023] Figure 6 for Figure 5 Enlarged structural diagram at point A;

[0024] Figure 7 This is a schematic diagram of the longitudinal section structure of the injection cylinder in an embodiment of the present invention;

[0025] The symbols for the main components are explained below:

[0026] Rat tail 01, base 1, rubber pad 11, slide groove 12, slide rail 13, rack 14, bracket 15, fixing clamp 16, timer 17, bearing seat 21, bearing groove 22, base ring 3, first ring body 31, traction frame 311, first mounting seat 32, sliding frame 321, first gear 33, transmission groove 331, second gear 34, transmission wheel 341, hexagonal cylinder 36, first sleeve 361, hose 362, first drug delivery cylinder 363, transmission rope 37, movable ring 4, second ring body 41, drive ring 42, gear ring 43, flexible coating 44, injection cylinder 5, outer cylinder 51, second drug delivery cylinder 52, positioning sleeve 53, piston 54, connecting rod 55, movable claw 56, positioning block 57. Detailed Implementation

[0027] To enable those skilled in the art to better understand the present invention, the technical solution of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0028] like Figure 1 , Figure 2 As shown, a hair removal device for experimental mice used in vitiligo research includes a base 1 and a drug delivery device. The base 1 is provided with a rack 14 and a bracket 15. A fixing clip 16 is installed on the bracket 15. A support seat 21 is installed on the base 1. A sliding groove 12 is opened on the base 1. The support seat 21 is slidably locked in the sliding groove 12. The support seat 21 is provided with a receiving groove 22. When applying the drug, the receiving groove 22 is used to place the mouse tail 01. A rubber pad 11 is fixedly installed at the bottom of the base 1 to increase the frictional resistance of the lower part of the base 1 and prevent the base from sliding during use.

[0029] like Figures 3-5As shown, the drug delivery device includes a base ring 3, a movable ring 4, and an injection cylinder 5. The movable ring 4 is rotatably mounted on the base ring 3. The base ring 3 includes a first ring body 31, on which a first mounting seat 32 and a traction frame 311 are provided. The base ring 3 is moved by holding the traction frame 311. A first gear 33 and a second gear 34 are mounted on the first mounting seat 32. The first gear 33 and the second gear 34 are perpendicular to each other. A transmission groove 331 is opened on the first gear 33. A transmission wheel 341 is coaxially connected to the second gear 34. A transmission rope 37 is wound between the first gear 33 and the transmission groove 331. The transmission rope 37 can make the perpendicular first gear 33 and the second gear 34 move together.

[0030] The movable ring 4 includes a second ring body 41, which is rotatably mounted on the first ring body 31. The second ring body 41 is provided with a gear ring 43 that meshes with the second gear 34. When the first gear 33 rotates, it drives the second gear 34 to rotate through the transmission rope 37, thereby driving the second ring body 41 to rotate. A drive ring 42 with a transmission screw on the end face is installed on the second ring body 41. A through hole is axially opened on the first ring body 31. A first sleeve 361 is fixedly installed in the through hole. A first drug delivery cylinder 363 is slidably installed in the through hole. A hose 362 is connected between the first sleeve 361 and the first drug delivery cylinder 363. The hose 362 is used to deliver hair removal cream and can also prevent the first drug delivery cylinder 363 from leaving the through hole. At the same time, the first drug delivery cylinder 363 will not cause noise to the hose 362 when it moves and squeezes the hose 362. The first drug delivery cylinder 363 is movable and can be used for rat tails of different thicknesses.

[0031] like Figure 6 , 7 As shown, the injection cylinder 5 includes an outer cylinder 51, a piston 54, and a connecting rod 55. The hair removal cream is contained inside the outer cylinder 51. A second drug delivery cylinder 52 is provided on the outer cylinder 51. When the connecting rod 55 drives the piston to move, the hair removal cream can be squeezed out from the second drug delivery cylinder 52. The second drug delivery cylinder 52 is inserted into the first sleeve 361. A movable claw 56 and a positioning block 57 are installed on the connecting rod 55. The movable claw 56 has a toothed groove that matches the transmission screw on the drive ring 42. The drive ring 42 is connected to the movable claw 56 in a transmission manner. The positioning block 57 is axially fitted with the outer cylinder 51. A flexible coating 44 is installed on the second ring body 41. The flexible coating 44 rotates with the second ring body 41 to evenly apply the squeezed hair removal cream to the surface of the rat tail 01.

[0032] Specifically, the base ring is provided with a hexagonal cylinder 36 corresponding to the through hole, and the outer cylinder 51 is provided with a positioning sleeve 53 that matches the hexagonal cylinder 36. The positioning sleeve 53 is fitted on the hexagonal cylinder 36 to correspond with the injection cylinder 5, so that the movable claw 56 matches the second ring body 41, and at the same time, the injection cylinder 5 is more stable after installation to avoid shaking during use.

[0033] Specifically, the base 1 is provided with a slide rail 13, and the first mounting base 32 is provided with a sliding frame 321 that matches the slide rail 13. The sliding frame 321 is slidably locked on the slide rail 13. The medication applicator is stabilized by the sliding frame 321, so that no manual stabilization is required when moving the medication applicator to apply hair removal cream, which is convenient to use.

[0034] Specifically, the second gear 34 is mounted on the first mounting base 32 via a rotating shaft. A ratchet mechanism is installed between the transmission wheel 341 and the rotating shaft. Under the action of the ratchet mechanism, the transmission wheel 341 can only perform unidirectional transmission to the rotating shaft, thereby preventing the medication from driving the movable ring 4 to rotate and causing the movable claw 56 to retract when the medication retracts along the rack 14, which would cause the piston 54 to retract and affect subsequent use.

[0035] Specifically, the first ring body 31 is provided with two hexagonal cylinders 36, both of which are located in the upper half of the first ring body 31. The included angle between the two hexagonal cylinders 36 is in the range of 70° to 90°. In this embodiment, the included angle between the two hexagonal cylinders 36 is preferably 75°. Both hexagonal cylinders 36 are equipped with injection tubes 5, which can increase the dosage. At the same time, the hair removal cream that is squeezed out is located in the upper half of the rat tail and is not easy to fall off, which facilitates the subsequent application of the flexible smear 44.

[0036] Specifically, a timer 17 is installed on the base 1. After applying the hair removal cream to the rat's tail, the timer 17 can be used to better control the hair removal time.

[0037] In use, the depilatory cream is loaded into the syringe 5 and mounted on the hexagonal cylinder 36. The movable claw 56 is connected to the drive ring 42. Then, the applicator is moved close to the fixed clamp 16, and the rat's tail is placed on the receiving groove 22 with one end of the tail passing through the applicator and clamped on the fixed clamp 16. The applicator is moved along the slide rail 13 by holding the traction frame 311. During the movement, the first gear 33 rolls on the rack 14 and drives the second gear 34 to rotate via the transmission rope 37. The second gear 34 drives the second ring body 41 to rotate via the gear ring 43. The drive ring 42 drives the movable claw 56 to move, causing the piston 54 to squeeze out the depilatory cream and attach it to the rat's tail 01. At the same time, the flexible coating 44 rotates with the second ring body 41 and evenly spreads the squeezed-out depilatory cream on the surface of the rat's tail 01. The time is then preset on the timer 17 as needed. When the timer ends, the depilatory cream is wiped off with a tissue to complete the hair removal process.

[0038] The foregoing has provided a detailed description of a hair removal device for experimental mice used in vitiligo research, as provided by the present invention. The specific embodiments described are merely for the purpose of helping to understand the method and core ideas of the present invention. It should be noted that those skilled in the art can make various improvements and modifications to the present invention without departing from its principles, and these improvements and modifications also fall within the protection scope of the claims of the present invention.

Claims

1. A hair removal device for laboratory mice used in vitiligo research, characterized in that: The hair removal device includes a base (1) and a drug delivery device. The base (1) is provided with a rack (14) and a bracket (15). A fixing clip (16) is installed on the bracket (15). A support seat (21) is slidably installed on the base (1). The support seat (21) is provided with a groove (22). The drug delivery device includes a base ring (3), a movable ring (4), and an injection cylinder (5). The movable ring (4) is rotatably installed on the base ring (3). The base ring (3) includes a first ring body (31). 31) is provided with a first mounting base (32) and a traction frame (311). A first gear (33) and a second gear (34) are mounted on the first mounting base (32). A transmission groove (331) is opened on the first gear (33). A transmission wheel (341) is coaxially connected to the second gear (341). A transmission rope (37) is wound between the first gear (33) and the transmission groove (331). The movable ring (4) includes a second ring body (41). The second ring body (41) is provided with a connection to the second gear (341). 34) Engaging gear ring (43), a drive ring (42) with a transmission screw on the end face is installed on the second ring body (41), a through hole is axially opened on the first ring body (31), a first sleeve (361) is fixedly installed in the through hole, a first drug delivery cartridge (363) is slidably installed in the through hole, a hose (362) is connected between the first sleeve (361) and the first drug delivery cartridge (363), the injection cartridge (5) includes an outer cylinder (51), a piston (54) and a connecting rod (55), the The outer cylinder (51) is provided with a second drug delivery cylinder (52), which is inserted into the first sleeve (361). The connecting rod (55) is equipped with a movable claw (56) and a positioning block (57). The movable claw (56) is provided with a toothed groove that matches the transmission screw on the drive ring (42). The drive ring (42) is connected to the movable claw (56) in a transmission manner. The positioning block (57) is axially fitted with the outer cylinder (51). The second ring body (41) is equipped with a flexible coating (44).

2. The experimental mouse hair removal device for vitiligo research according to claim 1, characterized in that: The base ring is provided with a hexagonal cylinder (36) corresponding to the through hole, and the outer cylinder (51) is provided with a positioning sleeve (53) that matches the hexagonal cylinder (36).

3. The experimental mouse hair removal device for vitiligo research according to claim 2, characterized in that: The base (1) is provided with a slide rail (13), and the first mounting base (32) is provided with a sliding frame (321) that matches the slide rail (13). The sliding frame (321) is slidably locked onto the slide rail (13).

4. The experimental mouse hair removal device for vitiligo research according to claim 3, characterized in that: The second gear (34) is mounted on the first mounting base (32) via a rotating shaft, and a ratchet mechanism is installed between the transmission wheel (341) and the rotating shaft.

5. The experimental mouse hair removal device for vitiligo research according to claim 4, characterized in that: The first ring body (31) is provided with two hexagonal cylinders (36), both of which are located in the upper half of the first ring body (31). The included angle between the two hexagonal cylinders (36) is 70° to 90°, and each of the two hexagonal cylinders (36) is equipped with an injection cylinder (5).

6. The experimental mouse hair removal device for vitiligo research according to claim 5, characterized in that: A timer (17) is mounted on the base (1).

7. The experimental mouse hair removal device for vitiligo research according to claim 1, characterized in that: A rubber pad (11) is fixedly installed below the base (1).

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