reagent bottle

Abstract

The application discloses a reagent bottle, comprising: a tube body, the tube body is internally provided with an extraction cavity for reaction and extraction, a sample cavity for sample feeding and a waste liquid cavity for waste liquid collection, the sample cavity and the waste liquid cavity are communicated with the extraction cavity through micro flow channels; a tube cap, the tube cap is suitable for closing the tube body; wherein, at least one of the tube cap and the tube body is internally provided with a closed reagent cavity, and the tube cap is configured to be movable when closing the tube body to be suitable for puncturing the reagent cavity, and the reagent cavity is configured to be suitable for reagent flowing into the sample cavity and / or the extraction cavity after being punctured. According to the reagent bottle of the embodiment of the application, reagent and sample can be fed into the sample cavity and then enter the extraction cavity for reaction and extraction, and the excessive reagent can enter the waste liquid cavity, so that the pressure in the extraction cavity is prevented from being too high.

Description

Technical Field

[0001] This invention relates to the field of medical technology, and in particular to a reagent bottle. Background Technology

[0002] In the medical diagnostics industry, reagent-based test results are often required to diagnose illnesses, such as COVID-19. For accuracy and stability, samples are typically collected via swabs and then extracted using reagents. Existing testing devices are relatively large, and the testing process requires different negative pressure spaces. Furthermore, the open testing method can easily affect the results and may lead to environmental contamination. Summary of the Invention

[0003] One objective of this invention is to provide a reagent bottle that seals the tube body with a cap and allows for reagent addition by moving the cap, thereby completing extraction and reaction under closed-tube conditions and avoiding sample or environmental contamination.

[0004] According to an embodiment of the present invention, a reagent bottle includes: a tube body, wherein the tube body is provided with an extraction chamber for reaction and extraction, a sample chamber for sample delivery, and a waste liquid chamber for waste liquid collection, wherein the sample chamber and the waste liquid chamber are both connected to the extraction chamber via microchannels; a cap, wherein the cap is adapted to close the tube body; wherein at least one of the cap and the tube body is provided with a closed reagent chamber, and the cap is configured to be movable when sealing the tube body to puncture the reagent chamber, and the reagent chamber is configured to allow reagent to flow into the sample chamber and / or the extraction chamber after puncture.

[0005] According to the reagent bottle of the present invention, the tube body is sealed by a cap, and the reagent is added by moving the cap, thereby completing the extraction and reaction under closed tube conditions and avoiding sample contamination or environmental contamination.

[0006] In addition, the reagent bottle according to the above embodiments of the present invention may also have the following additional technical features:

[0007] Optionally, the extraction chamber is provided with an extraction membrane, the waste liquid chamber is connected to the inner side of the extraction membrane, and the sample chamber is connected to the outer side of the extraction membrane.

[0008] Optionally, the tube body includes an inner end plate and an outer end plate, with the extraction cavity formed between the inner end plate and the outer end plate. A first through hole and a second through hole are formed on the inner end plate. The first through hole connects the sample cavity and the extraction cavity, and the second through hole connects the waste liquid cavity and the extraction cavity.

[0009] Optionally, the inner end plate is provided with a plurality of first through holes and a plurality of second through holes, the plurality of first through holes being spaced apart along the circumference of the inner end plate, and the plurality of second through holes being spaced apart along the circumference of the inner end plate.

[0010] Optionally, the distance between the first via and the sample cavity is greater than the distance between the second via and the sample cavity.

[0011] Optionally, the outer side of the inner end plate is provided with a receiving groove, and the outer end plate covers the receiving groove to form an extraction cavity. The bottom surface of the receiving groove is provided with crisscrossing flow grooves, and the second through hole connects to the flow grooves.

[0012] Optionally, the inner side of the outer end plate is provided with a central groove and a second guide groove. The central groove forms part of the extraction cavity, and the second guide groove connects to the central groove and extends in a divergent manner away from the central groove. The second guide groove connects to the first through hole.

[0013] Optionally, the tube body is provided with a sleeve, a sample chamber is constructed inside the sleeve, a waste liquid chamber is constructed between the sleeve and the tube body, a first end of the tube body is provided with an annular plate, the inner circumference of the annular plate is connected to the sleeve, the outer circumference of the annular plate is connected to the tube body, a plurality of first guide grooves are provided on the outer end face of the annular plate, the plurality of first guide grooves are all connected to the sample chamber and extend in a divergent manner in a direction away from the sample chamber, and the first guide grooves are connected to the first through hole.

[0014] Optionally, the annular plate is provided with a third through hole, which is spaced apart from the first guide groove and is directly opposite to the second through hole. The second through hole and the third through hole cooperate to connect the extraction chamber and the waste liquid chamber.

[0015] Optionally, the extraction cavity is located at the first end of the tube to facilitate detection.

[0016] Optionally, the cap is placed over the second end of the tube body, and the cap is adapted to move along the axis of the tube body.

[0017] Optionally, the tube body has a puncture structure, and the tube cap has a closed reagent chamber. The tube cap is movably connected to the tube body to accommodate the puncture structure puncturing the reagent chamber and connecting the reagent chamber with the sample chamber for closed-tube extraction.

[0018] Optionally, the cap is provided with a plurality of reagent chambers at intervals, the plurality of reagent chambers being adapted to be punctured sequentially by the puncturing structure and connected sequentially to the sample chamber, the cap being movably connected to the tube body along the axial direction of the tube body, and the plurality of reagent chambers being arranged at intervals along the axial direction of the cap.

[0019] Optionally, the tube body is further provided with a piston chamber and a piston rod, and the piston chamber is connected to the extraction chamber. The inner end face of the piston rod is provided with a piercing structure. The tube cap is movably connected to the tube body to facilitate pushing the piston rod to pierce the reagent contained in the piston chamber for closed-tube extraction. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of a detection device according to an embodiment of the present invention.

[0021] Figure 2 This is a schematic diagram of the detection device according to an embodiment of the present invention after the casing has been removed.

[0022] Figure 3 This is a partial schematic diagram of a detection device according to an embodiment of the present invention.

[0023] Figure 4 This is a schematic diagram of a portion of the detection device according to an embodiment of the present invention in another direction.

[0024] Figure 5 This is a schematic diagram of the mounting base and positioning components of a detection device according to an embodiment of the present invention.

[0025] Figure 6 This is a schematic diagram of the mounting base, heating element, and hot air module of the testing equipment according to an embodiment of the present invention.

[0026] Figure 7 This is a schematic diagram of the mounting base of a detection device according to an embodiment of the present invention.

[0027] Figure 8 This is a partial schematic diagram of a detection device according to an embodiment of the present invention.

[0028] Figure 9 yes Figure 8 This diagram shows the structure after the mounting shell has been removed.

[0029] Figure 10 This is a schematic diagram of the combination of the turntable, probe, and filter assembly in a detection device according to an embodiment of the present invention.

[0030] Figure 11 This is a schematic diagram of the combination of a turntable, probe, filter assembly and sensing element in a detection device according to an embodiment of the present invention.

[0031] Figure 12 This is a schematic diagram of the inner side of the power connection structure of a detection device according to an embodiment of the present invention.

[0032] Figure 13 This is a schematic diagram of a reagent bottle according to an embodiment of the present invention.

[0033] Figure 14 This is a cross-sectional view of a reagent bottle according to an embodiment of the present invention.

[0034] Figure 15 yes Figure 14 A magnified view of a portion of region A in the middle circle.

[0035] Figure 16 This is a cross-sectional view of a reagent bottle according to an embodiment of the present invention.

[0036] Figure 17 This is a schematic diagram of the diaphragm of a reagent bottle according to an embodiment of the present invention.

[0037] Figure 18 This is a schematic diagram of the diaphragm of a reagent bottle according to an embodiment of the present invention.

[0038] Figure 19 This is a schematic diagram of the tube body of a reagent bottle according to an embodiment of the present invention.

[0039] Figure 20 This is a schematic diagram of the tube body of a reagent bottle according to an embodiment of the present invention.

[0040] Figure 21 This is a schematic diagram of the tube body of a reagent bottle according to an embodiment of the present invention.

[0041] Figure 22 This is a schematic diagram of the inner side of the inner end plate of a reagent bottle according to an embodiment of the present invention.

[0042] Figure 23 This is a schematic diagram of the outer side of the inner end plate of a reagent bottle according to an embodiment of the present invention.

[0043] Figure 24 This is a schematic diagram of the inner side of the outer end plate of a reagent bottle according to an embodiment of the present invention. Detailed Implementation

[0044] Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, and should not be construed as limiting the present invention.

[0045] The detection device 200 according to an embodiment of the present invention includes a positioning module, which is provided with an insertion port 201 for inserting a reagent bottle 100. After the reagent bottle 100 is inserted, operations such as locking, heating, heat sealing, and quantitative fluorescence detection can be performed on the reagent bottle 100. After the reagent bottle is inserted into the insertion port, the positioning module can lock the reagent bottle. The different operating devices of the reagent bottle 100 will be described below with reference to the accompanying drawings.

[0046] Combination Figures 1 to 7 This invention describes an apparatus for heating a reagent bottle 100 according to one embodiment, including a plurality of heating elements 22 and a driving device 23. The plurality of heating elements 22 are movable to selectively heat the extraction membrane in the reagent bottle 100. That is, all of the plurality of heating elements 22 can heat the extraction membrane in the reagent bottle 100. Generally, to facilitate temperature control, one or more of the plurality of heating elements 22 can be used to heat the reagent bottle 100 to avoid damaging the reaction reagents, prevent fatigue, and improve the service life of the detection device 200. The driving device 23 is connected to the heating elements 22 to drive the heating elements 22 to move.

[0047] According to the present invention, the detection device 200 selectively heats the extraction membrane in the reagent bottle 100 by setting multiple heating elements 22, thereby satisfying different detection temperatures and improving the applicability of the detection device 200. At the same time, it can meet the detection requirements of the same detection target under multiple temperature conditions.

[0048] The heating element 22 in this invention can move in multiple directions to move closer to and further away from the reagent bottle 100, that is, switch between heating the reagent bottle 100 and not heating the reagent bottle 100. For example, the heating element 22 can be rotated to move closer to and further away from the reagent bottle 100; it can also be configured to move radially along the insertion hole to move closer to and further away from the reagent bottle 100. In addition, this invention provides some embodiments to heat the reagent bottle 100.

[0049] In some embodiments of the present invention, the heating element 22 is movably disposed on the positioning module in a direction parallel to the axis of the insertion port 201. That is, the heating element 22 moves in a direction parallel to the axis of the insertion port 201 to move closer to and further away from the reagent bottle 100, thereby realizing the heating and non-heating of the reagent bottle 100. This avoids interference between the heating element 22 and other devices (such as the device for rotating the tube cap, the device for locking the tube body, etc. described below), improves the stability of the movement of the heating element 22, simplifies the structure of the detection device 200, and facilitates the design, production and maintenance of the detection device 200.

[0050] like Figure 5The heating element 22 has a heating end for heating the reagent bottle 100. The heating end is positioned close to or against the reagent bottle 100 to heat it. The heating end is opposite to the insertion port 201 along the axis of the insertion port 201, and the projections of the heating ends of the plurality of heating elements 22 onto the axis of the insertion port 201 form a ring with an observation port. This facilitates heating of the reagent bottle 100, and by providing an observation port, interference with the inspection device (e.g., probe 272 described below) can be avoided. This allows for convenient observation while facilitating heating, thereby improving detection efficiency.

[0051] like Figure 6 The driving device 23 includes multiple heating elements 22, each corresponding to one of them. The driving device 23 includes a heating motor and a heating cam. The heating cam is rotatably embedded in the corresponding heating element 22. The heating motor is connected to the heating cam and is used to drive the corresponding heating element 22 to move. Specifically, the heating element 22 can be provided with a insertion hole, the size of which can be larger than the size of the heating cam. Since the heating cam is a non-rotating body relative to the motor shaft of the heating motor, there are different positions on the outer peripheral surface of the heating cam relative to the motor shaft, thereby achieving the driving of the heating element 22. Of course, other structures in the prior art can also be used in this invention to drive the heating element 22.

[0052] like Figure 6 and Figure 7 The positioning module includes a mounting base 26, a heating element 22, and a driving device 23, all mounted on the mounting base 26 and positioned away from the insertion port 201. The mounting base 26 has a mating hole 203, into which the heating element 22 is adapted to extend to heat the reagent bottle 100. By providing the mounting base 26, the integration of the heating device can be facilitated, enabling the assembly and maintenance of the testing equipment 200, and ensuring the stability of the movement of the heating element 22.

[0053] like Figure 5 The mounting base 26 is equipped with a positioning component, which is opposite to the insertion port 201 and is adapted to accommodate and position the end of the reagent bottle 100. During use, the reagent bottle is inserted into the insertion port 201, and the end of the reagent bottle is inserted into the positioning component. The positioning component positions the reagent bottle, thereby fixing the tube body of the reagent bottle and facilitating the rotation of the tube cap.

[0054] Among them, such as Figure 5 The positioning assembly may include a positioning ring 211, which is adapted to receive the end of the reagent bottle 100. During use, the end of the reagent bottle is inserted into the positioning ring 211.

[0055] Optionally, such as Figure 5The positioning ring 211 has a positioning rib 212 on its inner circumferential surface. The positioning rib 212 extends in a direction parallel to the axis of the insertion port 201 and is used to position the reagent bottle 100 circumferentially around the insertion port 201. Correspondingly, the reagent bottle 100 has a positioning groove on its body. The positioning groove can be a structure adapted to the positioning rib 212. During assembly, the reagent bottle 100 can be inserted into the insertion port 201, and the positioning rib 212 can be inserted into the positioning groove to fix the body of the reagent bottle 100. The positioning rib 212 can be set to be asymmetrical with respect to the axis of the insertion port 201. In this way, the reagent bottle 100 will be inserted into the insertion port 201 in a specific orientation. For example, during the heat sealing process, by limiting the orientation of the reagent bottle 100, the heat sealing needle can be easily inserted into the reagent bottle 100 to complete the heat sealing of the corresponding opening inside the reagent bottle 100.

[0056] Optionally, such as Figure 5 The positioning assembly also includes a pin 213, which is radially movably connected to the mounting base 26 along the positioning ring 211. The pin 213 is used to position the reagent bottle 100 along the axial direction of the insertion port 201. Correspondingly, an anti-dislodgement groove can be provided at the end of the reagent bottle 100. During use, the pin 213 can be inserted into the anti-dislodgement groove to achieve positioning of the reagent bottle 100 along the axial direction of the insertion port 201, preventing the reagent bottle 100 from falling out of the insertion port 201.

[0057] In this invention, reagent dispensing, reagent mixing, and reaction can be completed between the reagent bottle 100 being inserted into the detection device 200, or the reagent bottle 100 can be inserted into the detection device 200 and the actual dispensing can be achieved through the corresponding structure on the detection device 200.

[0058] Combination Figure 3 and Figure 4 In some embodiments of the present invention, the positioning module further includes a rotating cylinder 214 and a driving structure 215. An insertion port 201 is formed within the rotating cylinder 214, and the rotating cylinder 214 is rotatable about the axis of the insertion port 201. The driving structure 215 is connected to the rotating cylinder 214 to drive the rotating cylinder 214 to rotate. The rotating cylinder 214 is adapted to lock the cap of the reagent bottle 100 to drive the cap to rotate. During use, the reagent bottle 100 is inserted into the positioning module. The reagent bottle 100 includes a tube body and a cap. Reagent dispensing is achieved by rotating the cap relative to the tube body. The positioning components above can fix the tube body of the reagent bottle 100, and by locking and rotating the rotating cylinder, the cap can rotate relative to the tube body, thus completing the reagent dispensing. Therefore, by setting the rotating cylinder 214 and the driving structure 215, automatic dispensing of reagents from the reagent bottle 100 can be achieved.

[0059] Optionally, the heating element 22 of the present invention is a constant temperature module. The constant temperature module can heat the extraction membrane in the reagent bottle 100, so as to heat the extraction membrane to a predetermined temperature to complete the detection, improve the detection effect, and at the same time avoid fatigue caused by excessive heating, thereby improving structural strength and service life.

[0060] In addition, the present invention provides several technical solutions for heat-sealing the opening inside the reagent bottle 100. In some embodiments of the present invention, the detection device 200 further includes a heat-sealing device 24 and a heat-sealing drive 25. The heat-sealing device 24 is opposite to the insertion port 201 along the axial direction of the insertion port 201. The heat-sealing device 24 is movably disposed within the positioning module along the axial direction of the insertion port 201. The heat-sealing device 24 includes a heat-sealing needle adapted to be inserted into the heat-sealed reagent bottle 100. The heat-sealing drive 25 is disposed on the positioning module and connected to the heat-sealing device 24 to drive the heat-sealing device 24 to move. After the reagent bottle 100 is inserted into the detection device 200, the heat-sealing needle can be inserted into the reagent bottle 100 by moving the heat-sealing device 24, thereby completing the heat sealing of the corresponding opening inside the reagent bottle 100 and improving the accuracy of the detection results.

[0061] Optionally, such as Figure 6 The heat sealing drive 25 includes a heat sealing motor and a heat sealing cam. The heat sealing cam is rotatably embedded in the heat sealing device 24. The heat sealing motor is connected to the heat sealing cam and is used to drive the heat sealing device 24 to move. The driving method of the heat sealing drive 25 is basically the same as that of the heating drive.

[0062] Optionally, in conjunction with the foregoing description, the positioning module includes a mounting base 26, with a heat-sealing device 24 disposed on the mounting base 26 and facing away from the insertion port 201. The mounting base 26 has a mating hole 203, and the heat-sealing device 24 is adapted to extend into the insertion port 201 through the mating hole 203. This facilitates the integration of various components in the testing equipment 200, and makes assembly and maintenance easier. In conjunction with the foregoing embodiments, both the heat-sealing device 24 and the heating element 22 are disposed on the mounting base 26.

[0063] like Figure 5 In some embodiments of the present invention, a limiting plate 261 is provided within the mating hole 203. The limiting plate 261 is a hollow annular shape, and a guide hole 262 corresponding to the heat-sealing needle is provided on the limiting plate 261. This guides the heat-sealing needle and improves the stability of the heat-sealing effect. A guide cylinder, a hollow cylindrical structure, can also be provided on the limiting plate 261. The heat-sealing cover can pass through the guide cylinder to guide the heat-sealing needle. The guide cylinder is embedded within the heating element 22, thereby guiding the heating element 22. Thus, the guide cylinder simultaneously guides both the heating cylinder and the heat-sealing needle, simplifying and optimizing the structure.

[0064] In addition, the detection device 200 of the present invention also includes a device for observing the reagent bottle 100, and by observing the reagent bottle 100 under different temperature and other conditions, a reasonable and accurate conclusion is finally given.

[0065] like Figures 8 to 12 In some embodiments of the present invention, the imaging module includes a turntable 271, a probe 272, multiple filter assemblies 273, and a sensing element 274. The turntable 271 is rotatably connected to a positioning module. The probe 272 is fixed relative to the positioning module and faces the end of the insertion port 201. The multiple filter assemblies 273 are arranged at intervals around the rotation axis of the turntable 271. The multiple filter assemblies 273 are driven by the turntable 271 to selectively face the probe 272. The sensing element 274 is configured to cooperate with the probe 272 to capture the light after passing through the filter assemblies 273. During use, the reagent bottle 100 can be observed using different filter assemblies 273 by rotating the turntable 271 to meet the detection requirements and improve the accuracy of the detection results.

[0066] In this configuration, a sensing element 274 can be correspondingly provided on each filter assembly 273 to improve detection efficiency. Simultaneously, a sensing element 274 can be provided to observe the morphological effects under different filter assemblies 273. Optionally, combined with... Figure 10 and Figure 11 The sensing element 274 is fixed relative to the positioning module and opposite to the probe 272. Multiple filter assemblies 273 are driven by the turntable 271 to selectively align with the probe 272 and the sensing element 274. By fixing the sensing element 274 to the positioning module, the wiring of the sensing element 274 can be simplified, avoiding wire tangling during the rotation of the turntable 271, effectively improving the stability of signal transmission. At the same time, the number of sensing elements 274 can be reduced, thus lowering costs.

[0067] Combination Figure 11 and Figure 12In some embodiments of the present invention, the turntable 271 is provided with multiple mutually insulated electrical connection structures. Each electrical connection structure includes an insulating post 281, an electrical connection ring 282, and a conductive element 283. The insulating post 281 is fixed relative to the positioning module. The electrical connection ring 282 is rotatably sleeved on the insulating post 281. The conductive element 283 is disposed on the insulating post 281 and slidably abuts against the electrical connection ring 282. The conductive element 283 is electrically connected to the electrical connection ring 282. The filter assembly 273 is electrically connected to the electrical connection ring 282. The conductive element 283 can be connected to a power source via a wiring harness. Since the insulating post 281 is fixedly connected to the positioning module, the electrical connection between the conductive element 283 and the corresponding structure on the positioning module is positioned, preventing wire entanglement. The contact ring 282 can be fixedly connected to the turntable 271. Since the conductive part 283 abuts against the contact ring 282, the two can achieve a stable electrical connection during relative movement, thereby achieving stable power connection of the conductive part 283 and connecting the filter assembly 273 to the power supply.

[0068] In addition, an insulating ring 284 can be provided between the conductive component 283 and the turntable 271 to electrically isolate the conductive component 283 and the turntable 271, thereby improving the safety of the electrical connection structure.

[0069] The insulating post 281 has a hollow structure and a through hole on its circumference. The conductive element 283 is a ball-headed plunger located inside the insulating post 281 and extending out of the through hole. The ball head of the ball-headed plunger abuts against the conductive element 283. This improves the stability of the conductive structure.

[0070] Optionally, such as Figure 11 The turntable 271 includes a hub 2711 and multiple filter holders 2712. The multiple filter holders 2712 are connected to the hub 2711 and arranged around the hub 2711. Multiple filter assemblies 273 are matched one-to-one with the multiple filter holders 2712.

[0071] In addition, the hub 2711 has mounting slots at both ends, and electrical connection structures are installed in the mounting slots.

[0072] like Figure 8 In some embodiments of the present invention, the detection device further includes a support base 208 and a mounting shell 209, with the mounting shell 209 covering the support base 208. The sensing element 274 is disposed on the support base 208, the probe 272 is disposed on the mounting shell 209, and the turntable 271 and multiple filter assemblies 273 are all disposed within the mounting shell 209. This allows for stable installation of each component, optimizing the structure and stability.

[0073] Alternatively, the insulating posts 281 of the two electrical connection structures can be connected to the support base 208 and the mounting shell 209 respectively. This achieves a stable connection between the insulating posts 281 and the positioning module.

[0074] Alternatively, a circular cavity can be provided within the mounting housing 209, and the turntable 271 can be rotatably disposed within the circular cavity. The turntable 271 can be positioned through the circular cavity, guiding its stable rotation.

[0075] Optionally, the support base 208 is provided with a rotating shaft, and the turntable 271 includes a turntable seat and a turntable cover. The turntable seat is rotatably connected to the rotating shaft, and the turntable seat has multiple mounting slots arranged around the rotating shaft for mounting the filter assembly 273. The turntable cover covers the turntable seat and the mounting slots. By setting up the turntable seat and the turntable cover, damage to the filter assembly 273 by other components can be avoided, and the influence of the surrounding environment on the observation results can be effectively eliminated, thereby improving the accuracy of the observation results.

[0076] Optionally, the turntable cover includes a base plate and multiple partitions. The base plate covers the turntable base and has holes corresponding one-to-one with the multiple filter assemblies 273. The partitions are connected to the base plate and extend toward the turntable base, and the multiple partitions are spaced apart circumferentially along the base plate. A partition is provided between every two adjacent filter assemblies 273. The base plate allows for the encapsulation of multiple filter assemblies 273, while the partitions facilitate the separation of the multiple filter assemblies 273, reducing the entry of dust and other impurities into the filter assemblies 273 and improving the accuracy of the test results.

[0077] like Figure 11 In some embodiments of the present invention, the turntable 271 further includes an external gear ring 2713, which surrounds and is fixedly connected to the turntable base. A turntable motor is provided on the support base or mounting shell, and the turntable is driven by the turntable motor. The turntable motor is connected to the external gear ring 2713 for transmission. This facilitates the rotation of the turntable 271 and improves the stability of the turntable 271 during rotation.

[0078] Combination Figures 1 to 12 In conjunction with the preceding embodiments, the positioning module also includes a mounting base 26. The mounting base 26 is equipped with a heating device for heating the reagent bottle 100. The mounting base 26 is located between the turntable 271 and the insertion port 201. The mounting base 26 has a mating hole 203. The insertion port 201, the mating hole 203, and the probe 272 are aligned along the axis of the insertion port 201. The heating device can heat the extraction membrane, thereby obtaining good detection results. Simultaneously, placing the mounting base 26 between the turntable 271 and the insertion port 201 simplifies the structure of the heating device and the observation device. The mating hole 203 on the mounting base 26 allows light to pass through, facilitating observation and improving detection efficiency.

[0079] In this invention, the mounting base 26 and the support base 208 can be assembled separately and then assembled together, thereby simplifying the assembly process.

[0080] like Figure 1In some embodiments of the present invention, the positioning module includes a housing 21, with an insertion port 201 protruding from the outer surface of the housing 21. The housing 21 is provided with a heat dissipation vent communicating with the internal space of the housing 21. By providing a heat dissipation vent, heat dissipation can be conveniently achieved for the components within the positioning module, optimizing the working environment of each component in the testing device 200.

[0081] The positioning module, heating module, and shooting module are all located inside the casing.

[0082] Optionally, the detection device 200 also includes a display screen 29, and the display surface of the display screen 29 is configured as part of the appearance surface of the positioning module.

[0083] In addition, the detection device also includes a signal processing module, which is located on the positioning module and transmits signals with the sensing element 274; the display screen 29 transmits signals with the signal processing module. By integrating the display screen 29 onto the housing 21, the detection device 200 can be easily miniaturized and made portable.

[0084] As can be seen from the foregoing description, the detection device 200 of this invention can be used for PCR detection, and can improve the detection efficiency of PCR and the portability of the detection device 200. The detection process can be divided into the following steps:

[0085] ① The mixing of sample and reagent is completed in reagent bottle 100. That is, the reagent is added to the cavity where the sample is located, and the sample is extracted and collected by the extraction membrane. This process can be completed before the reagent bottle 100 is inserted into the detection device 200, or the reagent bottle 100 can be inserted into the detection device 200 and the detection device 200 can automatically complete the reagent dispensing process. The addition of reagent and the extraction process of the extraction membrane will be described in detail below.

[0086] ② After the reagent bottle 100 is inserted into the insertion port 201, the reagent bottle 100 is locked and positioned by the pin 213, positioning rib 212 and other structures, so that the reagent bottle 100 can be inserted into the detection device 200 according to the predetermined trajectory and direction.

[0087] ③ After the reagent bottle 100 is inserted and the reagent is added, the reagent bottle 100 can be heat-sealed to seal the extraction chamber in the reagent bottle 100, so that the multi-component actual storage reaction can be carried out in the extraction chamber as a whole, and the extraction chamber can be isolated from the sample chamber and the waste liquid chamber.

[0088] ④ Subsequently, the extraction membrane in the reagent bottle 100 can be heated using the heating element 22. Since both the extraction membrane and the outer end plate are relatively thin, the entire extraction membrane can be heated by heating one part of the extraction membrane. Three heating elements 22 (or one, two, four or more heating elements 22) can be set to heat the reagent bottle 100. The heating element 22 can be a constant temperature module. The heating element 22 can move up and down along the axis of the insertion port 201 to heat or not heat the reagent bottle 100. The multiple heating elements 22 are evenly spaced around the insertion port 201. For example, the Sagan heating elements 22 are arranged at 120° intervals. The program can enable multiple heating elements 22 to heat the extraction membrane in the reagent bottle 100 separately.

[0089] ⑤ After heating is complete or during heating, the reagent bottle 100 can be observed through the probe 272, filter assembly 273, and sensing element 274. The filter assembly 273 can be switched by rotating the turntable 271, and this switching can be done by continuous unidirectional rotation, shortening the wavelength switching time. Power can be supplied through a slip-ring power supply structure. Five different colored fluorescent filter assemblies 273 can be set to improve detection accuracy.

[0090] The above steps enable reagent detection and improve detection efficiency. In this invention, the microscopic optical path is miniaturized, a highly sensitive color camera is used, and a colorless fluorescent filter assembly 273 is employed to capture fluorescence images of each fluorescence channel.

[0091] In conjunction with the aforementioned testing device 200, the present invention also provides a reagent bottle 100, which can be applied to the aforementioned testing device 200.

[0092] like Figure 13As shown, the present invention also provides a reagent bottle 100, which can be used in the aforementioned detection equipment, or can be manufactured, sold, and used independently. It includes a tube body 11 and a cap 12. The tube body 11 has an extraction chamber, a sample chamber, and a waste liquid chamber, both of which are connected to the extraction chamber via microchannels. The extraction chamber is used for reaction and extraction, the sample chamber is used for sample loading, and the waste liquid chamber is used for waste liquid collection. The cap 12 is adapted to seal the tube body 11. At least one of the cap and the tube body has a sealed reagent chamber, and the cap is configured to be movable when sealing the tube body to puncture the reagent chamber, and the reagent chamber is configured to allow reagent to flow into the sample chamber and / or the extraction chamber after puncture. Therefore, in the reagent chamber of the present invention, after the tube body is sealed by the cap, the reagent can still be added by moving the cap, thereby realizing closed-tube reagent addition, closed-tube extraction and closed-tube reaction. For example, in PCR detection, sample extraction and reaction are all carried out in the detection tube, which can effectively avoid the influence of the external environment on the detection process, and can also avoid the generation of aerosols during the detection process from affecting the surrounding environment.

[0093] In addition, in conjunction with the previous embodiments, after the reagent bottle 100 is inserted into the insertion port, the extraction chamber of the reagent bottle 100 can be opposite to the heating module and the imaging module, thereby realizing the heating and imaging of the extraction membrane of the reagent bottle 100, thus achieving effective quantitative fluorescence detection. The process of quantitative fluorescence detection can be completed with one device, simplifying the detection.

[0094] In some embodiments of the present invention, a sleeve 111 is provided inside the tube body 11. An extraction chamber is provided at the first end of the tube body 11 (refer to the lower end of the tube body 11 in the accompanying drawings). A sample chamber 1102 is constructed inside the sleeve 111. A waste liquid chamber is constructed between the sleeve 111 and the tube body 11. Both the waste liquid chamber and the sample chamber are connected to the extraction chamber via microchannels. A cap 12 seals the second end of the tube body 11 (refer to the upper end of the tube body 11 in the accompanying drawings). A sample can be placed in the sample chamber 1102. For example, a collected sample swab A can be placed in the sample chamber 1102. To reduce the size of the reagent bottle 100, the sample swab A, such as a cotton swab, can be broken off, and the effective part placed in the sample chamber 1102. The cap 12 seals the tube body 11 and can move along the tube body 11. Thus, reagent dispensing can be achieved by moving the cap 12 along the tube body 11. This invention provides different implementation methods for reagent dispensing. For example, the reagent can be placed inside the cap 12 and pierced through the sleeve 111; alternatively, the reagent can be placed inside the tube body 11, and dispensing can be achieved by moving the cap 12. During reagent dispensing, the cap 12 seals the tube body 11, which can prevent the generation of aerosols and other contaminants, avoid contamination of the sample and reagents inside the reagent bottle, and also prevent contamination of the external environment. This invention will be described in detail below.

[0095] This invention employs a closed reagent addition method, which prevents contamination within the bottle and to the external environment, while also reducing the impact of the external environment on the extraction and reaction processes. This allows for storage and operation at moderate temperatures or room temperature.

[0096] like Figure 14 In some embodiments of the present invention, the cap 12 is provided with a closed reagent chamber 1201. The cap 12 is movably connected to the tube body 11. The tube body also has a piercing structure, which is adapted to pierce the reagent chamber 1201 during the movement of the cap 12 relative to the tube body 11, and connect the reagent chamber 1201 to the sleeve. This allows for reagent delivery and sample extraction even when the cap 12 is closed to the tube body 11, achieving closed-tube extraction. In other words, during the movement of the cap 12, the piercing structure in the tube body also moves relative to the cap 12 and pierces the reagent chamber 1201 inside the cap 12. After the reagent chamber 1201 is pierced, it can be connected to the sample chamber 1102, allowing the reagent in the reagent chamber 1201 to be delivered into the sample chamber 1102. The reagent in the reagent chamber 1201 can be a liquid reagent for convenient delivery.

[0097] There are several ways to construct the reagent chamber 1201 inside the cap 12 in this invention. For example, an air bladder can be provided inside the cap 12, or multiple partitions can be provided inside the cap 12 to construct multiple reagent chambers 1201 at intervals. Of course, in order to facilitate the puncture and connection of the cannula 111 to the reagent chamber 1201, an inner tube 121 can also be provided inside the cap 12 to construct the reagent chamber 1201. This invention will be described in detail below.

[0098] In some embodiments of the present invention, the cap may contain only one reagent chamber, or multiple reagent chambers may be spaced apart within the cap. These multiple reagent chambers are adapted to be sequentially punctured by the puncture structure and connected to the cannula in sequence. When only one reagent chamber is provided within the cap, reagent can be rapidly dispensed after the chamber is punctured. When multiple reagent chambers are present within the cap, the reagents within each chamber can be dispensed separately and sequentially.

[0099] The multiple reagent chambers inside the cap can be set up in various ways to facilitate the puncture of the puncture structure.

[0100] Optionally, the cap is movably connected to the tube body along the axial direction, and multiple reagent chambers are spaced apart along the axial direction of the cap. In this case, by moving the cap along the axial direction of the tube body, multiple reagent chambers can be punctured sequentially, and the multiple reagent chambers can be connected to the sample chamber in sequence, thereby realizing the release of reagents.

[0101] like Figures 14 to 16The cap 12 contains an inner tube 121 extending along the axis of the tube body 11. Multiple reagent chambers 1201 are spaced apart within the inner tube 121 along the axis of the tube body 11. The inner tube 121 is adapted to be inserted into the tube body 11 from its second end, allowing the sleeve 111 to sequentially pierce and connect the multiple reagent chambers 1201. By providing multiple reagent chambers 1201 within the inner tube 121, the dispensing of reagents within the chambers 1201 is convenient. Since the multiple reagent chambers 1201 are spaced apart along the axis of the tube body 11, the sleeve 111 can sequentially pierce the multiple reagent chambers 1201 during the movement of the cap 12, thereby enabling the separate dispensing of reagents within the chambers 1201 to meet different detection requirements.

[0102] Optionally, combined Figures 14 to 16 The inner tube 121 is closed at the end away from the sleeve 111. Multiple diaphragms 122 are provided inside the inner tube 121, spaced apart along the axis of the tube body 11. A reagent chamber 1201 is formed between each pair of adjacent diaphragms 122 and between each diaphragm 122 and the closed end of the inner tube 121. By providing diaphragms 122, it is easier to pierce the sleeve 111, further facilitating the piercing of the sleeve 111 and connecting the reagent chamber 1201, thereby improving reagent dispensing efficiency.

[0103] Furthermore, such as Figure 17 and Figure 18 The diaphragm 122 includes a rigid membrane layer 122a and a flexible membrane layer 122b. The rigid membrane layer 122a is connected around the flexible membrane layer 122b, and the rigid membrane layer 122a and the flexible membrane layer 122b are connected to form a flat plate. The flexible membrane layer 122b and the sleeve 111 are opposite each other in the axial direction of the tube body 11, and the thickness of the flexible membrane layer 122b is less than the thickness of the rigid membrane layer 122a. During use, the thinner flexible membrane layer 122b is opposite the sleeve 111. Therefore, during the movement of the tube cap 12, the sleeve 111 will easily puncture the flexible membrane layer 122b, further improving the reagent dispensing efficiency.

[0104] Optionally, the flexible membrane layer 122b and the hard membrane layer 122a are configured as a recessed structure with the opening facing the sleeve 111. During use, the end of the sleeve 111 will be inserted into the recessed structure. The recessed structure can guide the sleeve 111 so that the sleeve 111 can quickly and stably pierce the flexible membrane layer 122b, and also play a certain sealing role so that the reagent in the reagent chamber 1201 can be stably delivered into the sample chamber 1102.

[0105] Optionally, combined Figure 17 and Figure 18The flexible membrane layer 122b has multiple strip-shaped grooves 122c on its surface, which are connected at one point and arranged in a divergent pattern. By setting the strip-shaped grooves 122c, the flexible membrane layer 122b is made easier to puncture, improving the efficiency of reagent dispensing. Combined with... Figure 17 and Figure 18 It can be seen that at least one side surface of the flexible membrane layer 122b is provided with a strip groove 122c. In addition, the outermost diaphragm 122 on the inner tube is provided with multiple annular ribs, which can be multiple concentric circles extending from the sleeve.

[0106] Optionally, combined Figures 14 to 16 The inner tube 121 is configured as a variable-diameter tube with a gradually decreasing radial dimension in the direction away from the sleeve 111, and multiple steps are formed on the inner circumferential surface of the inner tube 121. The diaphragm 122 is disposed inside the sleeve 111 and supported on the steps on the inner circumferential surface of the inner tube 121. In other words, the inner tube 121 is configured to include multiple tube segments connected sequentially along the axial direction of the sleeve 111, and the radial dimension of the tube segment away from the sleeve 111 is smaller than the radial dimension of the tube segment closer to the sleeve 111, thereby forming a step facing the sleeve 111 between two adjacent tube segments. The diaphragm 122 can be supported on this step. During the process of piercing the diaphragm 122 through the sleeve 111, the step can provide support for the diaphragm 122, so that the sleeve 111 can stably pierce the diaphragm 122, thereby improving the stability of the piercing process.

[0107] In this invention, the inner tube 121 can be integrally formed with the cap 12, or the inner tube 121 and the cap 12 can be separately formed. Integral forming effectively improves the structural strength of the cap 12 and simplifies the forming process; however, integral forming places higher demands on the mold, resulting in higher initial mold costs. Therefore, this invention provides a structure where the cap 12 and the inner tube 121 are separately formed and then assembled together to simplify the production of the cap 12 and reduce costs. Specifically, as follows... Figure 14 and Figure 15 The inner end face of the cap 12 is provided with an annular groove, and the end of the inner tube 121 is provided with an annular hook 1202, which engages with the annular groove. Specifically, a rib structure can be provided on the inner end face of the cap 12 to construct the annular groove. The annular groove can be constructed to gradually shrink inward in the direction away from the inner end face of the cap 12, or the radial dimension of the annular groove can gradually decrease in the direction away from the inner end face of the cap 12. A structure corresponding to the annular groove can be provided on the inner shell. For example, the annular hook 1202 on the inner shell can be set to a gradually expanding shape. Through the cooperation of the annular groove and the annular hook 1202, a stable connection between the inner tube 121 and the cap 12 can be achieved, improving the connection strength between the inner tube 121 and the cap 12.

[0108] In addition, in this invention, multiple reagent chambers can also be spaced apart along the circumference of the cap, and the multiple reagent chambers can be punctured sequentially by rotating the cap relative to the tube body using the puncturing structure.

[0109] As described above, the cap of this invention can be provided with multiple reagent chambers, each of which can respectively hold a sample preservation solution, a lysis buffer, a first cleaning solution, a second cleaning solution, and an air column. These components are arranged sequentially within the cap for individual addition. The sample preservation solution, lysis buffer, first cleaning solution, second cleaning solution, and air column are arranged sequentially within the cap along the puncture sequence of the puncture structure. For example, in an embodiment where multiple reagent chambers are spaced apart along the axial direction of the tube body, the sample preservation solution, lysis buffer, first cleaning solution, second cleaning solution, and air column can be arranged sequentially within the multiple reagent chambers along the axial direction of the tube body. Furthermore, the reagent chamber for storing the sample preservation solution is located near the opening of the cap to facilitate the initial addition of the sample preservation solution into the sample chamber.

[0110] In this invention, the end of the sleeve can be constructed with the aforementioned puncture structure, thereby simplifying the tube body.

[0111] Among them, combined Figure 14 and Figure 21 To further facilitate the piercing of the reagent cavity 1201 by the sleeve 111, multiple notches 1103 spaced circumferentially along the end of the sleeve 111 can be provided. In this way, during the piercing of the reagent cavity 1201 by the sleeve 111, the side edges of the notches 1103 can provide multiple piercing points for the sleeve 111, thereby improving the piercing efficiency. Furthermore, the cap 12 in this invention can be threaded to the tube body 11, allowing the sleeve 111 to pierce the reagent cavity 1201 by rotating the cap. During this process, the rotation of the cap 12 allows the sleeve 111 to rotatably pierce the reagent cavity 1201. By providing the notches 1103, the force exerted on the reagent cavity 1201 by the periphery of the notches 1103 will exhibit excellent stress concentration, thus achieving rapid piercing of the reagent cavity 1201.

[0112] like Figure 14In some embodiments of the present invention, a sealing rib 1204 is provided on the inner end face of the cap 12. The sealing rib 1204 is adapted to abut against the inner circumference of the second end of the tube body 11 so that the cap 12 and the tube body 11 are sealed together. By providing the sealing rib 1204, when the cap 12 moves to a predetermined position along the axial direction of the tube body 11, for example when the end of the tube body 11 abuts against the inner end face of the cap 12, the sealing rib 1204 will fit against the inner circumference of the tube body 11, thereby achieving a seal between the cap 12 and the tube body 11, preventing reagent leakage from the reagent bottle 100, improving the accuracy of the test results, and avoiding waste.

[0113] In this invention, the cap 12 can be moved axially along the tube body 11 in a straight direction. Other methods can also be used to achieve the movement of the cap 12 relative to the tube body 11. Preferably, in one embodiment of this invention, the cap 12 is threadedly connected to the tube body 11. This not only facilitates a stable connection between the cap 12 and the tube body 11, but also allows the cap 12 to move relative to the tube body 11 through threaded transmission. Furthermore, threaded transmission allows the sleeve 111 to have greater force to pierce the reagent chamber 1201, improving structural stability and reagent dispensing efficiency. Figures 14 to 16 In some embodiments of the present invention, the cap 12 is fitted onto the outside of the pipe body 11. The inner circumferential surface of the cap 12 is provided with a first coarse thread 1205, and the outer circumferential surface of the pipe body 11 is provided with a second coarse thread 1104. The first coarse thread 1205 and the second coarse thread 1104 are engaged. Through the engagement of the first coarse thread 1205 and the second coarse thread 1104, a stable connection between the cap 12 and the pipe body 11 can be achieved.

[0114] The outer circumferential surface of the tube body 11 is also provided with fine threads, which mate with the second coarse thread 1104. When the sleeve 111 pierces the reagent cavity 1201, the first coarse thread 1205 contacts the fine thread. Through the engagement of the first coarse thread 1205 and the second coarse thread 1104, the cap 12 and the tube body 11 are threadedly engaged. The fine threads on the tube body 11 increase the resistance to rotating the cap 12, providing appropriate feedback to the user when manually rotating the cap 12. The placement of the fine threads in this application increases the resistance experienced by the cap 12 during the process of the sleeve 111 piercing the reagent cavity 1201, thus providing a clear indication to the user.

[0115] In addition, as mentioned above, the cap 12 of the present invention is provided with multiple reagent chambers 1201. By setting fine threads, it is convenient to remind users. If the required reagent type is less than the actual number of reagent chambers 1201, it is convenient for users to discover and control it in time.

[0116] In other words, during the rotation of the cap 12 in this invention, when the wall of the reagent chamber 1201 inside the cap 12 (as described above, the diaphragm 122) begins or is about to begin touching the sleeve 111, the first coarse thread 1205 will engage with the second coarse thread 1104 and the fine thread, thereby providing timely feedback to the user and making it easier for the user to understand the working status of the reagent bottle 100 so as to make adjustments.

[0117] Optionally, combined Figure 16 , Figures 22-24 The first end of the tube body 11 is provided with an inner end plate 112 and an outer end plate 113, forming an extraction cavity between the inner end plate 112 and the outer end plate 113. A first through hole 1106 is formed on the inner end plate 112, which connects the sample cavity 1102 and the extraction cavity. By setting the inner end plate 112 and the outer end plate 113, the first end of the tube body 11 can be easily sealed, and the structure of the extraction cavity is simplified, making it easier to form the tube body 11. In addition, an extraction membrane 118 can be placed in the extraction cavity, and the extraction membrane 118 can be held by the inner end cap and the outer end cap, improving the structural strength and stability of the reagent bottle 100.

[0118] The first and second through holes are suitable for heat sealing by a heat sealing device. Specifically, after the reagent bottle is inserted into the insertion port, a heat sealing needle can be inserted through the outer end plate into the inner end plate to seal the first and second through holes on the inner end plate.

[0119] Furthermore, to facilitate heating and observation of the reagent bottle, the positioning module in the aforementioned embodiments can be used to lock the reagent bottle. The positioning module can lock the body and cap of the reagent bottle, and can also drive the cap to move relative to the body to facilitate reagent dispensing. For example, the positioning module can drive the cap to rotate relative to the body.

[0120] Optionally, combined Figure 13 and Figure 19 An anti-detachment groove 1107 is provided on the outer circumferential surface of the first end of the tube body 11, and the anti-detachment groove 1107 is arranged along the circumference of the tube body 11. The anti-detachment groove 1107 can be an annular shape extending along the circumference of the tube body 11, or it can be a groove extending intermittently along the circumference of the tube body 11. When the tube body 11 is placed in the testing equipment, the tube body 11 can be axially positioned by the corresponding structure on the testing equipment to prevent the reagent bottle 100 from falling out of the testing equipment.

[0121] In conjunction with the preceding embodiments, the positioning module is adapted to cooperate with the anti-dislodgement groove to position the reagent bottle axially along the insertion port, wherein the pin in the aforementioned embodiments can be inserted into the anti-dislodgement groove to lock the reagent bottle.

[0122] In addition, anti-detachment protrusions or the like can be provided on the outer circumferential surface of the first end of the tube body 11. The axial positioning of the reagent bottle 100 can also be achieved through the anti-detachment protrusions or the like.

[0123] Optionally, combined Figure 19 A positioning groove 1108 can also be provided on the outer circumferential surface of the first end of the tube body 11. The positioning groove 1108 can extend in a direction parallel to the axis of the tube body 11. During use, positioning ribs can be set at the corresponding positions of the testing equipment to achieve circumferential positioning of the tube body 11.

[0124] The circumferential positioning of the pipe can be achieved through the aforementioned positioning module. For example, the aforementioned positioning rib can be embedded into the positioning groove to achieve circumferential locking of the pipe.

[0125] Optionally, such as Figure 13 The outer circumferential surface of the cap 12 is provided with multiple grooves 1206, which are parallel to the axis of the tube body 11 and are spaced apart along the circumference of the cap 12. The multiple grooves 1206 can increase the friction of the outer circumferential surface of the cap 12, and can improve the stable operation of the reagent bottle 100 when the cap 12 is moved along the axis of the tube body 11 by manual or automatic drive.

[0126] The toothed groove 1206 can be used to improve the stability of the manually or mechanically rotating cap 12. For example, the positioning module can cooperate with the toothed groove to achieve circumferential locking of the cap, and then the reagent can be dispensed by rotating the cap.

[0127] The toothed groove 1206 on the outer circumferential surface of the cap 12 can be configured to extend in a direction parallel to the axis of the tube body 11.

[0128] Optionally, such as Figure 13 and Figure 19 The outer circumferential surface of the first end of the tube body 11 is provided with anti-slip ribs 1109. The anti-slip ribs 1109 extend in a direction parallel to the axis of the tube body 11, and multiple anti-slip ribs 1109 are provided at intervals along the circumference of the tube body 11. The anti-slip ribs 1109 can increase the friction of the tube body 11. During use, when rotating the tube cap 12, force needs to be applied to both the tube cap 12 and the tube body 11. Therefore, by providing a toothed groove 1206 on the tube cap 12 and anti-slip ribs 1109 on the tube body 11, the rotation of the tube cap 12 relative to the tube body 11 can be facilitated.

[0129] Of course, other structures can also be provided in this invention to improve the friction of the outer peripheral surface of the cap 12 and the outer peripheral surface of the tube body 11. For example, groove-shaped, protruding or other shaped anti-slip structures can be provided on the outer peripheral surface of the cap 12 and the outer peripheral surface of the tube body 11.

[0130] In addition, the extraction chamber of the present invention can be equipped with a replaceable extraction membrane, or the extraction membrane can be pre-placed in the extraction chamber for easy use, avoiding pollution from the external environment. The extraction chamber is equipped with an extraction membrane, the waste liquid chamber is connected to the inner side of the extraction membrane, and the sample chamber is connected to the outer side of the extraction membrane.

[0131] As mentioned above, the cap 12 of the present invention is provided with a reagent cavity 1201. In other embodiments of the present invention, a cavity can also be provided in the tube body 11 to place the reagent, or the reagent can be placed in both the tube body 11 and the cap 12.

[0132] like Figure 16 In some embodiments of the present invention, the tube body has a piston cavity 1110 and a piston rod 114. The piston cavity 1110 is connected to the extraction cavity, and the inner end face of the piston cavity 1110 is provided with a piercing structure. The cap 12 is adapted to press against the piston rod 114 so that the reagent in the piston cavity 1110 enters the extraction cavity. The piston cavity 1110 can be filled with reagent. When the cap 12 moves in a direction parallel to the axis of the tube body 11, it can press against the piston rod 114, thereby allowing the reagent in the piston cavity 1110 to enter the extraction cavity.

[0133] The piston chamber 1110 can be configured to hold reagents such as reactive enzymes (e.g., lyophilized powder). The reactive enzymes can be in powder or other forms. Additionally, reagents can be placed in both the cap 12 and the tube body 11, thereby expanding the applicability of the reagent bottle 100 in this invention. In actual use, the reagent in the piston chamber 1110 can be added after the reagent in the cap 12 has been added. That is, during the movement of the cap 12, after the sleeve 111 punctures all the reagent chambers 1201 within the cap 12, the cap 12 drives the piston rod 114 to inject the reagent in the piston chamber 1110 into the extraction chamber. Of course, the order in which the reagents in the cap 12 and the tube body 11 are added can be selected according to actual use.

[0134] Optionally, such as Figure 16 A sealing ring 115 is provided between the outer peripheral surface of the piston rod 114 and the inner peripheral surface of the piston cavity 1110, sealing the gap between the piston rod 114 and the piston cavity 1110. The sealing ring 115 seals the gap between the piston rod 114 and the piston cavity 1110, thereby preventing reagents from flowing out of the piston cavity 1110 and preventing items from entering the piston cavity 1110, thus improving the stability of the reagent bottle 100.

[0135] The piston rod 114 has a first sealing groove on its outer circumferential surface, into which a sealing ring 115 is embedded, and the sealing ring 115 protrudes from the outer circumferential surface of the piston rod 114. This allows the sealing ring 115 to be stably mounted on the piston rod 114. Additionally, the piston cavity 1110 has a second sealing groove on its inner circumferential surface. The portion of the sealing ring 115 protruding from the outer circumferential surface of the piston rod 114 is adapted to be embedded in the second sealing ring 115 and to slide out from the second sealing groove. Thus, before the piston rod 114 begins to move, the cooperation of the first sealing groove, the second sealing groove, and the sealing ring 115 achieves a stable seal between the piston rod 114 and the inner circumferential surface of the piston cavity 1110. When the piston rod 114 is moved by the cap 12, the sealing ring 115 can slide out from the second sealing groove, achieving stable extension and retraction of the piston rod 114. The piston rod 114 and the piston cavity 1110 are constructed in a structure similar to a syringe, facilitating the injection of reagents into the extraction chamber.

[0136] Of course, in this invention, the sealing ring 115 can also be fixed on the inner circumferential surface of the sealing cavity, so that the piston rod 114 is movable relative to the sealing ring 115.

[0137] Combination Figure 14 and Figure 16 In some embodiments of the present invention, an inner tube 121 is provided inside the cap 12, and the inner tube 121 extends along the axis of the tube body 11. The cap 12 is adapted to drive the inner tube 121 to press against the piston rod 114. This can improve the stability of driving the piston rod 114. In conjunction with the aforementioned embodiments, multiple reagent chambers 1201 are provided inside the inner tube 121. Therefore, while constructing multiple reagent chambers 1201 inside the cap 12, the inner tube 121 can also press against and drive the piston rod 114, thus optimizing and simplifying the structure of the reagent bottle 100.

[0138] Optionally, the inner tube 121 is provided with a plurality of reagent chambers 1201 spaced apart along the axis of the tube body 11. The inner tube 121 is adapted to be inserted into the tube body 11 from the second end of the tube body 11, so that the sleeve 111 can pierce and connect the plurality of reagent chambers 1201 in sequence and allow the liquid reagent in the reagent chambers 1201 to enter the sample chamber 1102.

[0139] As mentioned above, in combination Figures 13 to 24This invention provides a reagent bottle 100, wherein the sleeve 111 and the tube body 11 can be concentric cylindrical. The hollow structure of the sleeve 111 can construct the aforementioned sample cavity 1102, and a partition structure can be provided in the cavity structure between the sleeve 111 and the tube body 11 to construct a piston cavity 1110, and a piston rod 114 is provided, which can be telescopically inserted into the piston cavity 1110. The sleeve 111 and the tube body 11 can be integrally formed, and the partition structure that constructs the piston cavity 1110 can also be integrally formed with the sleeve 111 and the tube body 11. A second coarse thread 1104 and a fine thread are provided on the outer circumferential surface of the second end of the tube body 11, and the fine thread and the second coarse thread cooperate with each other. The cap 12 is rotatably fitted onto the tube body 11, and a first coarse thread 1205 is provided on the inner circumferential surface of the cap 12. The first coarse thread 1205 and the second coarse thread 1104 cooperate to realize the threaded transmission between the cap 12 and the tube body 11, while the fine thread on the outer circumferential surface of the tube body 11 can provide resistance to the rotation of the cap 12. A sealing rib 1204 is provided on the inner end face of the cap 12. When the tube body 11 is inserted into the top of the cap 12, the circumference of the second end of the tube body 11 can cooperate with the sealing rib 1204 to improve the sealing effect. An inner tube 121 can be provided inside the cap 12. The inner tube 121 is flared, and multiple diaphragms 122 are arranged at intervals inside the inner tube 121. The multiple diaphragms 122 construct multiple reagent chambers 1201 inside the inner tube 121, and different reagents can be stored in the multiple reagent chambers 1201. During use, the sample is placed inside the sleeve 111, and the cap 12 is placed on the tube body 11. The cap 12 is rotated until its center area aligns with the diaphragm 122. As the cap 12 rotates, the diaphragm 122 touches the sleeve 111, at which point the first coarse thread 1205 contacts the fine thread. Further rotation of the cap 12 causes the sleeve 111 to puncture the diaphragm 122, allowing the reagent in the reagent chamber 1201 to enter the sleeve 111 and mix with the sample in the sample chamber 1102. With the rotation of the cap 12, multiple reagent chambers 1201 can be punctured through the sleeve 111, thus enabling the dispensing of various reagents.

[0140] Meanwhile, when the cap 12 moves to the predetermined position, the cap 12 will contact the piston rod 114. At this time, the piston rod 114 will provide a reverse force to the cap 12, slowing down the movement of the cap 12, so as to facilitate the full mixing of reagents and samples and avoid the simultaneous mixing of multiple reagents affecting the detection effect. At the same time, reagents can also be placed in the piston chamber 1110. In this way, when the cap 12 moves to a specific position, the cap 12 can drive the piston rod 114 to inject the reagent in the piston chamber 1110 into the extraction chamber.

[0141] In this invention, the extraction cavity can hold test strips, extraction membrane 118, etc.

[0142] As previously described, this invention describes a method for adding reagents into the sleeve 111. Different reagents can be added to the sleeve 111 to complete the required detection. Furthermore, after the reagents added to the sleeve 111 and the sample are mixed, they can enter the extraction chamber. The following describes the entry and exit of reagents into the extraction chamber in some embodiments of this invention with reference to the accompanying drawings.

[0143] Combination Figure 14 , Figure 16 as well as Figures 22 to 24 In some embodiments of the present invention, a waste liquid chamber is constructed between the sleeve 111 and the tube body 11, and the extraction chamber is connected to the waste liquid chamber. A sample chamber 1102 is constructed inside the sleeve 111, and a waste liquid chamber is constructed outside the sleeve 111. During use, reagents can be added to the sample chamber 1102, and the reagents can enter the extraction chamber through the sample chamber 1102. Excess reagents can flow back into the waste liquid chamber through the extraction chamber, thereby improving the stability of the reagent bottle 100. An extraction membrane 118 (e.g., for nucleic acid extraction) can be installed inside the extraction chamber. The extraction membrane 118 facilitates the retention of reagents and samples within the extraction chamber, while excess reagents enter the waste liquid chamber, avoiding waste.

[0144] In some embodiments of the present invention, the first end of the tube body 11 is provided with an inner end plate 112 and an outer end plate 113, and an extraction chamber is formed between the inner end plate 112 and the outer end plate 113. A first through hole 1106 and a second through hole 1113 are formed on the inner end plate 112. The first through hole 1106 connects the sample chamber 1102 and the extraction chamber, and the second through hole 1113 connects the waste liquid chamber and the extraction chamber. By providing the first through hole 1106 and the second through hole 1113, the sample chamber 1102 and the waste liquid chamber can be easily connected to the extraction chamber, enabling reagents to flow in the sample chamber 1102, the extraction chamber, and the waste liquid chamber, completing the sample extraction and other work, and achieving the purpose of sample detection.

[0145] Optionally, such as Figure 22 and Figure 23 The inner end plate 112 is provided with a plurality of first through holes 1106 and a plurality of second through holes 1113. The plurality of first through holes 1106 are arranged at intervals along the circumference of the inner end plate 112, and the plurality of second through holes 1113 are arranged at intervals along the circumference of the inner end plate 112. This can improve the efficiency of reagent entering the extraction chamber and contacting the extraction membrane 118 in the extraction chamber, and can also facilitate the return of excess reagent to the waste liquid chamber.

[0146] Furthermore, the distance between the first through-hole 1106 and the sample chamber 1102 is greater than the distance between the second through-hole 1113 and the sample chamber 1102. In this case, after the reagent enters the extraction chamber, it will flow back to the waste liquid chamber after passing through the extraction membrane 118, thus preventing the reagent from directly flowing back to the waste liquid chamber through the extraction chamber and providing sufficient reaction time between the reagent and the sample.

[0147] Combination Figure 16 and Figure 21 In some embodiments of the present invention, a ring plate 116 is provided at the first end of the tube body 11. The inner circumference of the ring plate 116 is connected to the sleeve 111, and the outer circumference of the ring plate 116 is connected to the tube body 11. A plurality of first guide grooves 1119 are provided on the outer end face of the ring plate 116. The plurality of first guide grooves 1119 are all connected to the sample chamber 1102 and extend in a divergent manner in a direction away from the sample chamber 1102. The first guide grooves 1119 are also connected to the first through hole 1106. This can increase the time and distance that the reagent flows through during the process of entering and exiting the extraction chamber, providing more reaction time between the reagent and the sample, and enhancing the extraction effect.

[0148] Optionally, combined Figure 16 and Figure 21 The annular plate 116 is provided with a third through hole 1114, which is spaced apart from the first guide groove 1119 and is directly opposite to the second through hole 1113. The second through hole 1113 and the third through hole 1114 cooperate to connect the extraction chamber and the waste liquid chamber. This facilitates the connection between the extraction chamber and the waste liquid chamber, avoids excessive pressure caused by too much reagent in the extraction chamber, and allows other reagents to quickly enter the extraction chamber.

[0149] Optionally, such as Figure 23 The inner end plate 112 has a receiving groove 1115 on its outer side, and the outer end plate 113 covers the receiving groove 1115 to form an extraction chamber. The bottom surface of the receiving groove 1115 has crisscrossing flow channels 1116, and the second through hole 1113 connects to the flow channels 1116. This allows for easy placement of the extraction membrane 118 and other reagents into the extraction chamber, and further increases the coverage area of ​​the reagents within the extraction chamber, facilitating the extraction of the extraction membrane 118.

[0150] Combination Figure 23 As can be seen, the container 1115 is a circular groove, and a circular flow groove 1116 is provided on the bottom surface of the container. Multiple annular ribs are provided in the flow groove 1116. The multiple annular ribs are arranged at intervals from the inside to the outside, and multiple radial grooves are connected to the multiple annular ribs, so that the reagent can flow evenly to the extraction membrane in the extraction chamber.

[0151] like Figure 24 In some embodiments of the present invention, a central groove 1117 and a second guide groove 1118 are provided on the inner side of the outer end plate 113. The central groove 1117 forms part of the extraction cavity. The second guide groove 1118 is connected to the central groove 1117 and extends in a divergent manner in a direction away from the central groove 1117. The second guide groove 1118 is connected to the first through hole 1106.

[0152] The reagent bottle 100 according to an embodiment of the present invention has a structure with multiple diaphragms 122 separating the reagents, and is suitable for detection reactions that require the addition of detection reagents in stages. For example, nucleic acid detection of the novel coronavirus.

[0153] In terms of specific technical solutions, there can be many different modes: (1) such as Figure 14 The multi-layer design shown is that the rotating cap 12 is used to add samples by puncturing them step by step; (2) different reagents are sealed on the same plane, and the puncture structure is designed at the edge position. The first reagent is punctured by the downward displacement of the cap 12. Other reagent chambers 1201 can be punctured by rotating the cap 12 and the reagent separator membrane 122 is cut one by one.

[0154] The reagent bottle 100 of this invention can be equipped with a rotating scale to indicate the type of reagent to be added.

[0155] This invention integrates multi-component reagents into a single reaction tube. All reagents are stored in containers, and each component is physically separated using a "segmentation plate" method (which can be a thin film or sheet) to prevent cross-contamination. The position of each reagent component within the tube is set according to experimental needs. The quantity of each reagent component is also set according to experimental requirements. The sample can be placed into the sleeve 111 after opening the cap, and then the cap is screwed on. During use, displacement is achieved by rotating or pressing the screw thread, causing the sleeve 111 to puncture the septa 122 of each reagent component sequentially, allowing the reagents to flow into the sleeve 111 in order, pass through the sample, and then enter the extraction chamber for reaction. Reagent components that do not need to flow through the sample can flow into the extraction chamber through a separate microchannel. The extraction chamber is connected to the sample chamber 1102 and the waste liquid chamber via microchannels. Multiple sets of microchannels (multiple first through-holes 1106 and multiple second through-holes 1113) can achieve large particle filtration and prevent blockage at any single point.

[0156] An extraction membrane 118 can be installed inside the extraction chamber. This membrane 118 can be a thin sheet (optimally 0.2 mm in height), providing a large heating area, uniform heat conduction, and shortening the PCR reaction time. For particulate samples, the focal plane of the microscopic image remains stable. The cap 12 has a stepped design corresponding to the liquid height of each reagent component. When the cap reaches this position, resistance increases, serving as a positional indicator during manual operation. Position lines for the reagent components can be printed on the outer circumference of the tube body 11, also serving as a positional indicator during manual operation.

[0157] The reagent bottle 100 in this invention can be operated manually or by machine.

[0158] The tube body 11 and the cap 12 in this invention have a sealing design. The tube body 11 and the cap 12 are sealed after the last component reagent flows into the sleeve 111, and all reagents are sealed in the tube to prevent leakage. The waste liquid chamber can be filled with absorbent material (filter paper or sponge, etc.). When the waste liquid enters the waste liquid chamber during operation, it is absorbed by the absorbent material to prevent backflow into the microchannel.

[0159] This invention achieves nucleic acid extraction without the need for any auxiliary tools.

[0160] The PCR reagent bottle 100 of this invention focuses on the structure of the liquid flow channel, the compartments for dispensing enzyme and elution buffer, and the PCR reaction chamber. It is suitable for directly adding extracted nucleic acids. The enzymes dispensed in each reagent chamber 1201 are lyophilized. Each reagent chamber 1201 can contain: sample preservation solution, lysis buffer, washing buffer 1, washing buffer 2, elution buffer, and enzyme reaction system. The extraction chamber can serve as the PCR reaction chamber; the reaction solution can enter the extraction chamber from the sample chamber 1102 or from the extraction chamber into the waste chamber.

[0161] The reaction can be promoted by heating the extraction membrane in the extraction chamber at different temperatures, and detection can be achieved by observing it with different filters.

[0162] In addition, the present invention also provides a detection system, which includes: a positioning module, a heating module, an imaging module, and a reagent bottle. The reagent bottle is the reagent bottle according to the foregoing embodiment. The positioning module has an insertion port, and the first end of the reagent bottle is adapted to be inserted into the insertion port and positioned by the positioning module. The heating module is used to heat the extraction chamber. The imaging module is opposite to the end face of the first end of the reagent bottle to be adapted to acquire a quantitative fluorescence image.

[0163] The detection system according to embodiments of the present invention can achieve the addition of multiple reagents in a closed tube. After the addition of multiple reagents, the reaction and nucleic acid extraction can be carried out in the closed tube. Moreover, the reagent bottle can also serve as a reaction chamber. During the acquisition of quantitative fluorescence images, the extraction membrane does not need to be removed, and the acquisition and reaction can be carried out directly, thereby effectively improving the detection accuracy and avoiding contamination of the external environment and the influence of the external environment on the detection process.

[0164] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A reagent bottle, characterized in that, include: The tube body is provided with an extraction chamber for reaction and extraction, a sample chamber for sample delivery, and a waste liquid chamber for waste liquid collection. The sample chamber and the waste liquid chamber are both connected to the extraction chamber through microchannels. A cap, adapted to seal the tube body, having a sealed reagent chamber inside, and configured to be movable when sealing the tube body to puncture the reagent chamber, wherein the reagent chamber is configured to allow reagent to flow into the sample chamber upon puncture. The tube body is provided with a sleeve with an open end. A sample chamber is constructed inside the sleeve. A waste liquid chamber is constructed between the sleeve and the tube body. A piercing structure is constructed at the end of the sleeve for piercing the reagent chamber. The tube cap is movably connected to the tube body to be adapted to allow the piercing structure to pierce the reagent chamber and connect the reagent chamber with the sample chamber for closed-tube extraction.

2. The reagent bottle according to claim 1, characterized in that, The extraction chamber is equipped with an extraction membrane, the waste liquid chamber is connected to the inner side of the extraction membrane, and the sample chamber is connected to the outer side of the extraction membrane.

3. The reagent bottle according to claim 1, characterized in that, The extraction chamber is located at the first end of the tube body, and the tube cap is sealed at the second end of the tube body. The tube body includes an inner end plate and an outer end plate, and the extraction chamber is formed between the inner end plate and the outer end plate. A first through hole and a second through hole are formed on the inner end plate. The first through hole connects the sample chamber and the extraction chamber, and the second through hole connects the waste liquid chamber and the extraction chamber.

4. The reagent bottle according to claim 3, characterized in that, The inner end plate is provided with a plurality of first through holes and a plurality of second through holes. The plurality of first through holes are spaced apart along the circumference of the inner end plate, and the plurality of second through holes are spaced apart along the circumference of the inner end plate.

5. The reagent bottle according to claim 3, characterized in that, The distance between the first via and the sample cavity is greater than the distance between the second via and the sample cavity.

6. The reagent bottle according to claim 3, characterized in that, The inner end plate has a receiving groove on its outer side surface, and the outer end plate covers the receiving groove to form an extraction cavity. The bottom surface of the receiving groove has crisscrossing flow grooves, and the second through hole connects to the flow grooves.

7. The reagent bottle according to claim 3, characterized in that, The inner side of the outer end plate is provided with a central groove and a second guide groove. The central groove forms part of the extraction cavity. The second guide groove is connected to the central groove and extends in a divergent manner away from the central groove. The second guide groove is connected to the first through hole.

8. The reagent bottle according to claim 3, characterized in that, The first end of the tube is provided with an annular plate. The inner circumference of the annular plate is connected to the sleeve, and the outer circumference of the annular plate is connected to the tube. The outer end face of the annular plate is provided with a plurality of first guide grooves. The plurality of first guide grooves are all connected to the sample cavity and extend in a divergent manner in a direction away from the sample cavity. The first guide grooves are connected to the first through hole.

9. The reagent bottle according to claim 8, characterized in that, The annular plate is provided with a third through hole, which is spaced apart from the first guide groove and is directly opposite to the second through hole. The second through hole and the third through hole cooperate to connect the extraction chamber and the waste liquid chamber.

10. The reagent bottle according to any one of claims 1-9, characterized in that, The extraction chamber is located at the first end of the tube body for detection, and the tube cap is sealed at the second end of the tube body. The tube cap is adapted to move along the axis of the tube body.

11. The reagent bottle according to claim 1, characterized in that, The cap is provided with multiple reagent chambers at intervals. The multiple reagent chambers are adapted to be punctured sequentially by the puncturing structure and connected to the sample chamber sequentially. The cap is movably connected to the tube body along the axial direction of the tube body, and the multiple reagent chambers are arranged at intervals along the axial direction of the cap.

12. The reagent bottle according to any one of claims 1-9, characterized in that, The tube body is also provided with a piston chamber and a piston rod, and the piston chamber is connected to the extraction chamber. The inner end face of the piston rod is provided with a piercing structure. The tube cap is movably connected to the tube body to facilitate pushing the piston rod to pierce the reagent contained in the piston chamber for closed-tube extraction.

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