Portable water environment eDNA sampling machine

Abstract

The application discloses a portable water environment eDNA sampling machine and relates to the technical field of water environment sampling. The portable water environment eDNA sampling machine is characterized by the following: a filter assembly is arranged, when water passes through a PES filter, a filter head is separated from a liquid guide pipe and a hose by pulling the filter head, the filter head is then placed into a sealing bag for sealing and marking, a hydrophilic plastic can immediately dry and store eDNA at ambient temperature, the sample is then stored at room temperature and waits for batch processing, the top shell is first opened during batch processing, and the filter membrane is then extracted. This kind of mode reduces the possibility of sample contamination, does not need to prepare ethanol and other slow filtering work compared to the traditional mode, and improves work efficiency.

Description

Technical Field

[0001] This invention relates to the field of water environment sampling technology, specifically a portable water environment eDNA sampling machine. Background Technology

[0002] Environmental DNA refers to the sum of DNA fragments that can be directly extracted from environmental samples such as water, soil, air, and ice. eDNA technology is a newly emerging biological survey method that consists of three parts: eDNA acquisition, gene analysis, and result analysis.

[0003] Traditionally, when detecting eDNA in water, the eDNA filter membrane must be placed in an on-site chemical preservative or cold storage. This usually requires researchers to open the filter housing, fold the filter membrane with a pair of sterile tweezers, and then insert the filter membrane into a vial or bag containing DNA preservative. This method increases the risk of sample contamination and requires cumbersome steps such as refrigeration materials or liquid preservatives. The resulting single-use plastic waste is wasteful and not environmentally friendly. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a portable aquatic environment eDNA sampling machine, which solves the problems of traditional sampling methods being cumbersome and prone to increasing the risk of sample contamination.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a portable aquatic environment eDNA sampling device, comprising a body, wherein a storage bottle and a pump body are fixedly installed inside the body, a connecting pipe is fixedly installed between the storage bottle and the pump body, an exhaust pipe is fixedly installed on one side of the top surface of the storage bottle, a drive assembly is provided on one side of the inside of the body, a pipe housing assembly is provided inside the body, a filter assembly is provided on the side of the body, the filter assembly includes a filter head, a flexible tube is fixedly sleeved at the end of the filter head, a top shell is fixedly installed on the outside of the filter head, and a steel mesh support, a nylon mesh filter, and a PES filter are fixedly installed between the filter head and the top shell.

[0006] Preferably, the pipe storage assembly includes a positioning plate frame, which is fixedly installed on one side of the inner side of the machine body. The positioning plate frame has several symmetrically opened grooves inside, and each groove is provided with a limit rod. Each end of each limit rod is rotatably mounted with a slider. Each slider is slidably installed inside a corresponding groove. Each slider is fixedly installed with a support spring between itself and the inner wall of its corresponding groove. A liquid guide tube is provided on the side of the positioning plate frame. The liquid guide tube is wrapped around the outside of each limit rod and its ends are fixedly connected to the pump body and the top outer shell, respectively.

[0007] Preferably, a positioning head is fixedly installed at one end of the liquid guide tube, and the positioning head is inserted into the side of the machine body.

[0008] Preferably, the drive assembly includes a lithium battery, a sealed box is fixedly installed on one side of the body, the lithium battery is fixedly installed inside the sealed box, a sealed door is rotatably installed on the side of the body, and a number of heat dissipation vents are symmetrically opened inside the sealed door, and a power interface is provided on the top surface of the sealed box.

[0009] Preferably, the side of the sealed box is kept sealed by the cooperation of the sealing door, and the power interface is electrically connected to the pump body by a wire.

[0010] Preferably, liquid level observation windows are symmetrically provided on both sides of the interior of the machine body, and a handle is rotatably installed on one side of the top surface of the machine body.

[0011] Beneficial effects

[0012] This invention provides a portable aquatic environment eDNA sampling device. Compared with the prior art, it has the following advantages:

[0013] Beneficial effects:

[0014] (1) This portable water environment eDNA sampling machine, through the setting of the filter component, after the water passes through the PES filter, pull the filter head to separate it from the liquid guide tube and the tubing, and then put the filter head into the sealed bag for sealing and marking. The hydrophilic plastic can immediately dry and preserve the eDNA at the ambient temperature, and then the sample is stored at room temperature for batch processing. When batch processing is carried out, the top shell is opened first, and then the filter membrane is extracted. This method reduces the possibility of sample contamination. Compared with the traditional method, there is no need to prepare ethanol and other slow filtration work, which improves the work efficiency.

[0015] (2) The portable water environment eDNA sampler, through the setting of the pipe storage component, when the filter head is pulled for operation, the liquid guide tube can be pulled outward, so that several limiting rods on the outside of the positioning plate frame can move downward through the end slider and the pulling of the liquid guide tube, and squeeze the corresponding support spring. When the end of the liquid guide tube is released after use, the several limiting rods can drive the liquid guide tube to reset through the cooperation of the slider and the support spring, realize the storage operation, and improve the ease of use of the equipment. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0017] Figure 2 For the present invention Figure 1 Schematic diagram of cross-section structure;

[0018] Figure 3 For the present invention Figure 1 Rear view structural diagram;

[0019] Figure 4 This is a schematic cross-sectional view of the positioning plate frame of the present invention;

[0020] Figure 5 This is a schematic diagram of the exploded structure of the filter component of the present invention.

[0021] In the diagram: 1. Main body; 101. Liquid level observation window; 102. Pump body; 2. Handle; 3. Storage bottle; 301. Exhaust pipe; 302. Connecting pipe; 4. Sealed box; 401. Power interface; 5. Lithium battery; 6. Sealed door; 601. Heat dissipation vent; 7. Positioning plate frame; 701. Slide groove; 8. Filter head; 801. Hose; 802. Top shell; 803. Steel mesh support; 804. Nylon mesh filter; 805. PES filter; 9. Limiting rod; 901. Slider; 10. Support spring; 11. Liquid guide tube; 1101. Positioning head. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Please see Figure 1-5 The present invention provides two technical solutions, specifically including the following embodiments:

[0024] Example 1:

[0025] A portable aquatic environment eDNA sampling device includes a body 1. A storage bottle 3 and a pump body 102 are fixedly installed inside the body 1. A connecting pipe 302 is fixedly installed between the storage bottle 3 and the pump body 102. An exhaust pipe 301 is fixedly installed on one side of the top surface of the storage bottle 3. A drive assembly is located on one side of the inside of the body 1. A pipe storage assembly is located inside the body 1. A filter assembly is located on the side of the body 1. The filter assembly includes a filter head 8. A flexible tube 801 is fixedly sleeved at the end of the filter head 8. A top outer shell 802 is fixedly installed on the outside of the filter head 8. A steel mesh support 803, a nylon mesh filter 804, and a PES filter 805 are fixedly installed between the top outer shell 802 and the pipe storage assembly. The pipe storage assembly includes a positioning plate frame 7, which is fixedly installed inside one side of the body 1. The positioning plate frame 7 has several symmetrically opened grooves 701 inside. Each groove 701 is provided with a limit rod 9. Each end of the limit rod 9 is rotatably mounted with a slider 901. The sliders 901 are slidably installed inside the corresponding grooves 701. The sliders 901 are in contact with the inner wall of the corresponding grooves 701. Each part is fixedly installed with a support spring 10. A liquid guide tube 11 is provided on the side of the positioning plate frame 7. The liquid guide tube 11 is wrapped around the outside of several limit rods 9, and its ends are fixedly connected to the pump body 102 and the top shell 802 respectively. A positioning head 1101 is fixedly installed at one end of the liquid guide tube 11. The positioning head 1101 is inserted into the side of the machine body 1. The drive component includes a lithium battery 5. A sealed box 4 is fixedly installed on one side of the inside of the machine body 1. The lithium battery 5 is fixedly installed inside the sealed box 4. A sealing door 6 is rotatably installed on the side of the machine body 1, and the inside of the sealing door 6 is symmetrically opened. Several heat dissipation vents 601 are provided. A power interface 401 is provided on the top surface of the sealed box 4. The sides of the sealed box 4 are kept sealed by the cooperation of the sealing door 6. The power interface 401 is electrically connected to the pump body 102 through a wire. Liquid level observation windows 101 are symmetrically opened on both sides of the interior of the body 1. A handle 2 is rotatably installed on one side of the top surface of the body 1. The filter head 8 is made of biodegradable hydrophilic plastic. Its function is to automatically preserve the captured eDNA by drying, absorb the remaining moisture in the packaging through the hydrophilic plastic, and preserve the captured eDNA on the filter membrane at the ambient temperature. Once in the laboratory, the top shell 802 can be easily opened for extraction; therefore, many field samples can be collected and transported in this way without the need for cumbersome refrigeration materials or liquid preservatives.

[0026] In use, through the filter assembly setup, when the pump body 102 is turned on, suction is generated inside the filter head 8 and its end hose 801 via the liquid guide tube 11. The hose 801 is then placed into the water to be tested. The water passing through the hose 801 passes through the steel mesh support 803, the nylon mesh filter 804, and the PES filter 805, until it reaches the storage bottle 3. The filter head 8 is then pulled to detach from the liquid guide tube 11, and the hose 801 at its end is removed. The filter head 8 is placed in a sealed bag and sealed. The hydrophilic plastic allows for immediate drying and preservation of the eDNA at ambient temperature. The sample is then stored at room temperature, awaiting batch processing. During batch processing, the top outer shell 802 is first opened, and then the eDNA filter membrane is used to extract DNA, reducing the sample temperature. This design increases the possibility of contamination, but compared to traditional methods, it eliminates the need for slow filtration processes like ethanol preparation, thus improving work efficiency. Through the design of the pipe storage component, when the filter head 8 is pulled, the liquid guide tube 11 can be pulled outwards. This causes several limiting rods 9 on the outside of the positioning plate frame 7 to move downwards via the end slider 901 and the liquid guide tube 11, compressing the corresponding support springs 10. After use, when the end of the liquid guide tube 11 is no longer under pressure, the limiting rods 9 can be reset through the cooperation of the slider 901 and the support springs 10, achieving storage and improving the ease of use of the equipment. The sealing box 4 and sealing door 6 on the outside of the lithium battery 5 ensure the sealing effect of the lithium battery 5, guaranteeing the safety of the equipment.

[0027] Example 2:

[0028] A portable water environment eDNA sampling device includes a body 1. A storage bottle 3 and a pump body 102 are fixedly installed inside the body 1. A connecting pipe 302 is fixedly installed between the storage bottle 3 and the pump body 102. An exhaust pipe 301 is fixedly installed on one side of the top surface of the storage bottle 3. A pipe storage assembly is provided inside the body 1. A filter assembly is provided on the side of the body 1. The filter assembly includes a filter head 8, with a flexible tube 801 fixedly connected to the end of the filter head 8. A top shell 802 is fixedly installed on the outside of the filter head 8. A steel mesh support 803, a nylon mesh filter 804, and a PES filter 805 are fixedly installed between the filter head 8 and the top shell 802. The pipe storage assembly includes a positioning plate frame 7, which is fixedly installed inside the body 1. Several sliding grooves 701 are symmetrically formed inside the positioning plate frame 7. Each of the sliding grooves 701 has a limit rod 9, and a slider 9 is rotatably mounted at the end of each limit rod 9. 01. Several sliders 901 are slidably installed inside the corresponding slide grooves 701. Support springs 10 are fixedly installed between the sliders 901 and the inner walls of the corresponding slide grooves 701. Liquid guide tubes 11 are provided on the side of the positioning plate frame 7. The liquid guide tubes 11 are wrapped around the outside of several limiting rods 9 and their ends are fixedly connected to the pump body 102 and the top shell 802 respectively. A positioning head 1101 is fixedly installed at one end of the liquid guide tube 11. The positioning head 1101 is inserted into the side of the body 1. A lithium battery 5 is fixedly installed inside the body 1. The lithium battery 5 is electrically connected to the pump body 102 through wires. Liquid level observation windows 101 are symmetrically opened on both sides of the inside of the body 1. A handle 2 is rotatably installed on one side of the top surface of the body 1. The filter head 8 is composed of biodegradable hydrophilic plastic. Its function is to automatically preserve the captured eDNA by drying, absorb the remaining moisture in the packaging through the hydrophilic plastic, and preserve the captured eDNA on the filter membrane at the ambient temperature. Once inside the laboratory, the top shell 802 can be easily opened and extracted; thus, many field samples can be collected and transported in this way without the need for cumbersome refrigeration materials or liquid preservatives.

[0029] In use, through the filter assembly setup, when the pump body 102 is turned on, suction is generated inside the filter head 8 and its end hose 801 via the liquid guide tube 11. The hose 801 is then placed into the water to be tested. The water passing through the hose 801 passes through the steel mesh support 803, the nylon mesh filter 804, and the PES filter 805, until it reaches the storage bottle 3. The filter head 8 is then pulled to detach from the liquid guide tube 11, and the hose 801 at its end is removed. The filter head 8 is placed in a sealed bag and sealed. The hydrophilic plastic allows for immediate drying and preservation of the eDNA at ambient temperature. The sample is then stored at room temperature, awaiting batch processing. During batch processing, the top outer... The shell 802 is then used to extract DNA through an eDNA filter membrane, reducing the possibility of sample contamination. Compared to traditional methods, it eliminates the need for slow filtration processes such as preparing ethanol, thus improving work efficiency. Through the design of the pipeline storage component, when the filter head 8 is pulled, the liquid guide tube 11 can be pulled outwards, causing several limiting rods 9 on the outside of the positioning plate frame 7 to move downwards through the end slider 901 and the pulling of the liquid guide tube 11, squeezing the corresponding support spring 10. When the end of the liquid guide tube 11 is released after use, the several limiting rods 9 can be reset by the cooperation of the slider 901 and the support spring 10, realizing the storage operation and improving the ease of use of the equipment.

[0030] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0031] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0032] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A portable aquatic environment eDNA sampling device, comprising a body (1), wherein a storage bottle (3) and a pump body (102) are fixedly installed inside the body (1), a connecting pipe (302) is fixedly installed between the storage bottle (3) and the pump body (102), an exhaust pipe (301) is fixedly installed on one side of the top surface of the storage bottle (3), and a drive assembly is provided on one side of the interior of the body (1), characterized in that: The body (1) is provided with a pipe storage assembly inside and a filter assembly is provided on the side of the body (1). The filter assembly includes a filter head (8), with a flexible tube (801) fixedly sleeved at the end of the filter head (8), and a top shell (802) fixedly installed on the outside of the filter head (8). A steel mesh bracket (803), a nylon mesh filter (804), and a PES filter (805) are respectively fixedly installed between the filter head (8) and the top shell (802). The pipe storage assembly includes a positioning plate frame (7), which is fixedly installed inside one side of the body (1). The positioning plate frame (7) has several symmetrically arranged grooves (701) inside. Each groove (701) has a limiting rod (9) inside, and each limiting rod (9) has a slider (901) rotatably mounted at its end. Each slider (901) is slidably installed inside its corresponding groove (701). A support spring (10) is fixedly installed between each slider (901) and the inner wall of its corresponding groove (701). A liquid guide tube (11) is provided on the side of the positioning plate frame (7). The liquid guide tube (11) is fixedly installed with a positioning head (1101) at one end, and the positioning head (1101) is inserted into the side of the body (1); the drive assembly includes a lithium battery (5); a sealed box (4) is fixedly installed on one side of the body (1); the lithium battery (5) is fixedly installed inside the sealed box (4); a sealed door (6) is rotatably installed on the side of the body (1); and several heat dissipation vents (601) are symmetrically opened inside the sealed door (6); and a power interface (401) is provided on the top surface of the sealed box (4).

2. The portable aquatic environment eDNA sampling device according to claim 1, characterized in that: The side of the sealed box (4) is kept sealed by the cooperation of the sealing door (6), and the power interface (401) is electrically connected to the pump body (102) by a wire.

3. The portable aquatic environment eDNA sampling device according to claim 1, characterized in that: The machine body (1) has symmetrical liquid level observation windows (101) on both sides inside, and a handle (2) is rotatably installed on one side of the top surface of the machine body (1).

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