Double "t" type quantitative sampling microfluidic chip and clamp applied to the chip

Abstract

The application relates to the field of microfluidic technology, in particular to a double-T-shaped quantitative sampling microfluidic chip and a clamp applied to the chip, which comprises a quantitative channel, a liquid inlet channel, a liquid outlet channel, a sample inlet channel and a sample outlet channel, the quantitative channel is communicated with the liquid inlet channel and the liquid outlet channel, or the quantitative channel is communicated with the sample inlet channel and the sample outlet channel, the quantitative channel is communicated with the liquid inlet channel and the liquid outlet channel or communicated with the sample inlet channel and the sample outlet channel, the conversion of the sample storage and the quantitative sampling functions is realized, the application has the advantages of simple structure, convenient use, cooperation with an external driving pump, only one flow switching channel is arranged on the microfluidic chip, when the application is used, the microfluidic chip can be converted between the sample storage state and the quantitative sampling state by changing the pressure difference of the driving pump, the measured sample liquid can be accurately and quantitatively taken, and the layout difficulty and the process machining difficulty of the microfluidic chip are reduced.

Description

Technical Field

[0001] This invention relates to the field of microfluidics, specifically to a dual "T"-shaped quantitative sampling microfluidic chip and a fixture applied to the chip. Background Technology

[0002] Microfluidic chips are a hot topic in the development of micro total analysis systems. The goal of microfluidic chips is to integrate the functions of the entire laboratory, including sampling, dilution, reagent addition, reaction, separation, and detection, onto a single microchip that can be used multiple times.

[0003] Microfluidic chips require a six-way valve and a metering loop for quantitative sampling during injection. The six-way valve injector is an ideal injector in a high-performance liquid chromatography (HPLC) system, consisting of a circular sealing gasket and a fixed base. The working principle of the six-way valve injector is as follows: When the handle is in the sampling position, the sample is injected into the metering loop through a micro-injection needle via the injection port. After the metering loop is full, excess sample is discharged through the vent port. When the handle is rotated to the injection position, the valve connects to the liquid flow path, and the mobile phase delivered by the pump flushes the metering loop, propelling the sample into the HPLC column for analysis.

[0004] While existing injection schemes can achieve high-precision sample liquid measurement and are relatively convenient to use, they still have problems: the six-way valve injector has a complex structure and is too large, making it difficult to integrate into a microfluidic system, which is not conducive to the miniaturization, portability and automation of the microfluidic system.

[0005] In addition, a crucial aspect of using microfluidic chips is the interface technology between macro-fluids and micro-fluids, i.e., how to inject macro-fluids into the micro-chip channels. The traditional method involves connecting an external sample inlet via a steel needle. However, this method has poor pressure resistance, the steel needle can easily damage the chip inlet when inserted, and the operation is complex and prone to introducing impurities.

[0006] Application content

[0007] The purpose of this invention is to provide a dual "T"-shaped quantitative sampling microfluidic chip and fixture to solve the problems mentioned in the background art, such as the complex structure, high cost, inconvenience in miniaturization and integration of existing quantitative sampling devices, and the damage to the chip entrance when the steel needle is inserted during sample injection.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] A dual "T"-shaped quantitative sampling microfluidic chip includes a quantitative channel, and also includes an inlet channel, an outlet channel, a sample inlet channel, and a sample outlet channel connected to the quantitative channel. The sampling is achieved by adjusting the pressure difference between the outlet channel and the sample outlet channel: The inlet end of the quantitative channel is connected to the inlet channel, and the outlet end of the quantitative channel is connected to the outlet channel; in this case, the microfluidic chip is in the liquid inlet state, and the sample inlet channel and the sample outlet channel are closed. Alternatively, the inlet end of the quantitative channel is connected to the sample inlet channel, and the outlet end of the quantitative channel is connected to the sample outlet channel; in this case, the microfluidic chip is in the sampling state, the quantitative channel is used as a quantitative sample outlet channel, and the inlet channel and the outlet channel are closed.

[0010] Furthermore, the sample inlet channel and the sample outlet channel are located at opposite ends of the quantitative channel, and the liquid inlet channel and the liquid outlet channel are located on the side wall of the quantitative channel, and both the liquid inlet channel and the liquid outlet channel are connected to the quantitative channel;

[0011] The inlet end of the liquid inlet channel is the liquid inlet, the outlet end of the liquid outlet channel is the liquid outlet, the inlet end of the sample inlet channel is the sample inlet, and the outlet end of the sample outlet channel is the sample outlet.

[0012] Furthermore, the inlet channel and the outlet channel are located on both sides of the metering channel, the inlet channel and the metering channel are in a "T" shape, and the outlet channel and the metering channel are in an inverted "T" shape.

[0013] Furthermore, both the inlet channel and the outlet channel are arranged perpendicular to the metering channel.

[0014] Furthermore, the dual "T"-shaped quantitative sampling microfluidic chip includes a cover layer, a trench layer, and a base layer arranged sequentially from top to bottom. The quantitative channel, inlet channel, outlet channel, and sample inlet channel are all cavities between the liquid flow channel on the trench layer and the cover layer. The cover layer is bonded to the trench layer. The inlet, outlet, sample inlet, and sample outlet are all located on the base layer. The inlet is connected to the quantitative channel through the inlet channel, the outlet is connected to the quantitative channel through the outlet channel, the sample inlet is connected to the quantitative channel through the sample inlet channel, and the sample outlet is connected to the quantitative channel through the sample outlet channel.

[0015] In addition, the present invention also provides a fixture for the aforementioned dual "T"-shaped quantitative sampling microfluidic chip, comprising a fixture box, a fixture base, and a fixture cover. The fixture box has an internal cavity, the fixture base is placed in the cavity of the fixture box, the microfluidic chip is placed on the fixture base, and the fixture cover is closed on the fixture box and abuts against the surface of the microfluidic chip.

[0016] Furthermore, the fixture base has a liquid channel communicating with the microfluidic chip. The upper surface of the fixture base has four interfaces communicating with the liquid channel. The liquid channel communicates with the inlet, outlet, sample inlet, and sample outlet of the microfluidic chip through the four interfaces. The position and size of the interfaces of the fixture base correspond to the position of the inlet, outlet, sample inlet, and sample outlet of the microfluidic chip, and are the same size. Each interface is equipped with a sealing gasket. The other end of the liquid channel is connected to an external liquid storage device or a drive pump.

[0017] Furthermore, the fixture cover has an observation window, which is located directly above the fixture base, and the edge of the observation window is provided with a buffer gasket.

[0018] Furthermore, the fixture base and the fixture box are detachably connected, and the bottom of the fixture box has an assist hole for removing the fixture base; the side wall of the fixture box has a communication hole for connecting the liquid flow channel inside the fixture base to the outside; the edge of the fixture box is provided with an arc-shaped groove for picking up the microfluidic chip.

[0019] Furthermore, the clamp cover is hinged to one side of the clamp box body via a hinge seat, and a push switch is provided on the other side of the clamp cover and clamp box body relative to the hinge seat. The push switch includes a push latch and a locking tongue. The push latch is provided on the clamp base, and the push locking tongue is provided on the clamp cover. The push latch and the push locking tongue are arranged opposite to each other.

[0020] Compared with the prior art, the beneficial effects of the present invention are:

[0021] (1) The present invention has a simple structure and is easy to use. The quantitative sampling microfluidic chip is equipped with only one flow switching channel, that is, the quantitative channel can be used as a sample storage channel and as a quantitative sample dispensing channel. When in use, in conjunction with an external drive pump, by changing the pressure of the external drive pump, the pressure difference between the liquid dispensing channel and the sample dispensing channel on the microfluidic chip can be controlled, so that the quantitative channel can be connected to the liquid inlet channel and the liquid outlet channel or to the sample inlet channel and the sample dispensing channel, thereby realizing the conversion of the sample storage and quantitative dispensing functions of the quantitative channel, and thus enabling the microfluidic chip to switch between the liquid inlet state and the sampling state, accurately measuring the quantitative sample liquid to be tested, reducing the layout difficulty and process difficulty of the microfluidic chip.

[0022] (2) This invention provides a fixture for a dual "T" type quantitative sampling microfluidic chip, which is adapted to the size of the microfluidic chip of this invention. The fixture can be tightly engaged and sealed with the microfluidic chip, avoiding the problem of damaging the chip inlet and introducing impurities when the steel needle is directly inserted into the microfluidic chip. In addition, an arc-shaped groove is provided for picking up the microfluidic chip, which facilitates the picking up of the microfluidic chip. A fixture cover is provided to tightly abut against the microfluidic chip, and the fixture cover is detachably connected to the fixture box body for easy opening and closing. An observation window is provided to facilitate observation of whether the state is switched. A communication hole is opened on the side of the fixture box body for connecting the liquid channel to an external liquid storage device or a drive pump, which facilitates the connection between the microfluidic chip and the external liquid storage device and sampling device. Attached Figure Description

[0023] Figure 1 An exploded view of the dual "T"-shaped quantitative sampling microfluidic chip of the present invention;

[0024] Figure 2 This is a top view of the dual "T"-shaped quantitative sampling microfluidic chip of the present invention.

[0025] Figure 3 This is a three-dimensional structural diagram of the clamp of the present invention;

[0026] Figure 4 This is a perspective view of the three-dimensional structure of the clamp base of the present invention;

[0027] Figure 5 This is a top view of the fixture base of the present invention.

[0028] Figure 6 This is a reference diagram showing the usage state of the present invention;

[0029] In the diagram: 1. Double "T" shaped quantitative sampling microfluidic chip; 11. Cover layer; 12. Groove layer; 121. Quantitative channel; 122. Liquid inlet channel; 123. Liquid outlet channel; 124. Sample inlet channel; 125. Sample outlet channel; 13. Substrate layer; 131. Liquid inlet; 132. Liquid outlet; 133. Sample inlet; 134. Sample outlet; 2. Fixture; 21. Fixture box; 211. Press-lock latch; 212. Assist hole; 213. Connecting hole; 214. Arc groove; 22. Fixture base; 23. Fixture top cover; 231. Observation window; 232. Press-lock tongue; 3. Drive pump; 4. Conduit; 5. Reaction cell. Detailed Implementation

[0030] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

[0031] Please see Figure 1-6 This invention provides a dual "T"-shaped quantitative sampling microfluidic chip, such as... Figure 1 As shown, this dual "T"-shaped quantitative sampling microfluidic chip 1 includes a cover layer 11, a trench layer 12, and a base layer 13 arranged sequentially from top to bottom. The cover layer 11, trench layer 12, and base layer 13 are bonded together by thermocompression bonding. A liquid flow channel is provided on the trench layer 12. The cover layer 11 is bonded to the trench layer 12. The cavity between the cover layer 11 and the trench layer 12 is respectively configured as a quantitative channel 121, a liquid inlet channel 122, a liquid outlet channel 123, a sample inlet channel 124, and a sample outlet channel 125. By adjusting the liquid outlet channel 125... The pressure difference between the sampling channel 121 and the inlet channel 122 and the outlet channel 123, or the sampling channel 121 and the inlet channel 124 and the outlet channel 125, enables the quantitative channel 121 to be connected to the inlet channel 122 and the outlet channel 123, or to the inlet channel 124 and the outlet channel 125. By selecting to connect with the inlet channel 122 and the outlet channel 123 or to the inlet channel 124 and the outlet channel 125, the quantitative channel 121 can switch between the sample storage and quantitative sampling functions, thereby enabling the microfluidic chip to switch between the inlet state and the sampling state.

[0032] like Figure 2As shown, the quantitative channel 121 has an inlet and an outlet at its two ends, respectively. An inlet channel 124 and an outlet channel 125 are located at opposite ends of the quantitative channel 121. An inlet channel 122 and an outlet channel 123 are located on opposite sides of the quantitative channel 121, and both are connected to the quantitative channel 121. Both are perpendicular to the quantitative channel 121, and the inlet channel 122 and outlet channel 123 form a "T" shape with the quantitative channel 121. Channel 123 and quantitative channel 121 form an inverted "T" shape. Those skilled in the art will know that inlet channel 122 and outlet channel 123 can also be located on the same side of quantitative channel 121, and the sidewalls of inlet channel 122 and outlet channel 123 can be inclined at a certain angle to the sidewalls of quantitative channel 121. In this embodiment, it is preferable that inlet channel 122 and outlet channel 123 are respectively located on both sides of quantitative channel 121, with both inlet channel 122 and outlet channel 123 perpendicular to quantitative channel 121. This vertical arrangement helps reduce channel length, channel pressure, and sample residue, and facilitates rinsing, making it the optimal layout. Furthermore, placing inlet channel 122 and outlet channel 123 on both sides of quantitative channel 121 facilitates the reasonable distribution and layout of external equipment or drive pumps, preventing misoperation. The inlet end of inlet channel 122 is inlet port 131, the outlet end of outlet channel 123 is outlet port 132, and the inlet end of sample injection channel 124 is... The sample port 133 and the outlet end of the sample outlet channel 125 are the sample outlet 134. The liquid inlet 131, liquid outlet 132, sample inlet 133 and sample outlet 134 are all located on the base layer 13. The liquid inlet 131 is connected to the quantitative channel 121 through the liquid inlet channel 122, the liquid outlet 132 is connected to the quantitative channel 121 through the liquid outlet channel 123, the sample inlet 133 is connected to the quantitative channel 121 through the sample inlet channel 124, and the sample outlet 134 is connected to the quantitative channel 121 through the sample outlet channel 125.

[0033] The working principle of this embodiment is as follows: When the inlet end of the quantitative channel 121 is connected to the liquid inlet channel 122 and the outlet end of the quantitative channel 121 is connected to the liquid outlet channel 123, the sample inlet channel 124 and the sample outlet channel 125 are closed and not conductive. At this time, the microfluidic chip is in the liquid inlet state, and the quantitative channel 121 is used as a sample storage channel. When the inlet end of the quantitative channel 121 is connected to the sample inlet channel 124 and the outlet end of the quantitative channel 121 is connected to the sample outlet channel 125, the liquid inlet channel 122 and the liquid outlet channel 123 are closed and not conductive. At this time, the microfluidic chip is in the sampling state, and the quantitative channel 121 is used as a quantitative sample outlet channel.

[0034] When using it, please refer to Figure 6The inlet 131 of the liquid inlet channel 122 is connected to an external liquid storage device, and the outlet 134 of the sample outlet channel 125 is connected to an external sampling device. A positive pressure is provided at the liquid inlet 131 by a drive pump, a negative pressure is provided at the outlet 132 of the liquid outlet channel 123 by a drive pump, and positive pressure is provided at the inlet 133 of the sample inlet channel 124 and the outlet 134 of the sample outlet channel 125 by a drive pump. Utilizing the pressure difference between the liquid outlet channel 123 and other liquid inlet channels 122, 124, and 125, the sample liquid to be tested enters the microfluidic chip through the inlet 131 of the liquid inlet channel 122. The quantitative channel 121 serves as a sample storage channel. When liquid enters the outlet channel 123, it indicates that the quantitative channel 121 is full. The sample solution to be tested is in the sample storage state of the microfluidic chip at this time. Then, positive pressure is provided by the driving pump at the liquid inlet 131, positive pressure is provided by the driving pump at the liquid outlet 132 of the liquid outlet channel 123, positive pressure is provided by the driving pump at the liquid inlet 133 of the sample inlet channel 124, and negative pressure is provided by the driving pump at the sample outlet 134 of the sample outlet channel 125. The pressure difference between the sample outlet channel 125 and other liquid inlet channels 122, liquid outlet channels 123 and sample inlet channels 124 causes the sample solution to flow out of the microfluidic chip from the quantitative channel 121 through the sample outlet 134 of the sample outlet channel 125. The quantitatively measured sample solution enters the reaction cell 5 for reaction (or enters other sampling devices). At this time, the quantitative channel 121 is used as a quantitative sample outlet channel.

[0035] This invention features a simple structure. With the external drive pump 3, the microfluidic chip has only one quantitative channel as a flow switching channel. That is, the quantitative channel can be used as a sample storage channel and also as a quantitative sample dispensing channel. In use, by changing the pressure provided by the drive pump 3 to the inlet 131, outlet 132, inlet 133, and outlet 134, the pressure difference between the outlet channel and other channels on the microfluidic chip (or the pressure difference between the outlet channel and other channels) can be controlled. This allows for the switching between the sample storage and quantitative sample dispensing functions of the quantitative channel, thereby enabling the microfluidic chip to switch between the sample storage state and the quantitative sampling state. This allows for the precise measurement of a quantitative amount of the sample liquid to be tested, reducing the layout and manufacturing difficulty of the microfluidic chip and facilitating miniaturization and integration.

[0036] The cover layer 11, the trench layer 12, and the base layer 13 are all organic polymer materials, preferably polymethyl methacrylate (PMMA), which is tough, hard, reasonably priced, and easy to machine. In particular, it has excellent biocompatibility, does not contaminate biological cells, and is transparent and easy to observe. However, it has poor scratch resistance and its interface is easily damaged by traditional steel needles during use.

[0037] To address the aforementioned dual "T"-shaped quantitative sampling microfluidic chip 1, and to prevent damage to the microfluidic chip interface during use, this invention also provides a fixture 2 for the dual "T"-shaped quantitative sampling microfluidic chip. Please refer to... Figure 3-5 As shown, the fixture 2 includes a fixture box 21, a fixture base 22, and a fixture cover 23. The fixture box 21 has an internal cavity for accommodating the chip, the size of which is consistent with the size of the microfluidic chip. The fixture base 22 is placed inside the cavity of the fixture box 21, and the microfluidic chip is placed on the fixture base 22. The fixture box 21 can be tightly engaged and sealed with the microfluidic chip to prevent the microfluidic chip from shaking inside the fixture box 21. The edge of the fixture box 21 is provided with arc-shaped grooves 214 for easy picking up of the microfluidic chip. In further optimization, there are two arc-shaped grooves 214, which are arranged opposite to each other on the edge of the fixture box 21, making it convenient to pick up, load, and adjust the position of the microfluidic chip from the side.

[0038] The fixture cover 23 is closed on the fixture box 21 and abuts against the surface of the microfluidic chip. The fixture cover 23 has an observation window 231, which is located above the fixture base 22. It is used to observe the liquid inlet or outlet state of the microfluidic chip and to determine whether to switch between liquid inlet state and sampling state. The outer edge of the observation window 231 is provided with a buffer gasket to increase the buffer and prevent damage to the microfluidic chip during the closing and tightening of the fixture cover 23.

[0039] The clamp cover 23 is hinged to one side of the clamp box 21 via a hinge seat. A push switch is provided on the other side of the clamp cover 23 and the clamp box 21 opposite to the hinge seat. The push switch includes a push latch 211 and a push tongue 232. The push latch 211 is provided on the clamp base 22, and the push tongue 232 is provided on the clamp cover 23. The push latch 211 and the push tongue 232 are arranged opposite to each other, and the push latch 211 and the push tongue 232 facilitate the opening and closing of the clamp cover 23.

[0040] The fixture base 22 is detachable from the fixture housing 21. The outer contour dimensions of the fixture base 22 are equal to the length and width of the accommodating cavity of the fixture housing 21. The fixture base 22 fits perfectly into the accommodating cavity of the fixture housing 21. The height of the fixture base 22 is slightly shorter than the accommodating cavity of the fixture housing 21. When the fixture base 22 is placed into the accommodating cavity of the fixture housing 21, the height difference between the upper surface of the fixture base 22 and the fixture housing 21 is exactly the thickness of the chip. A liquid channel communicating with the microfluidic chip is provided inside the fixture base 22. One end of the liquid channel extends to the upper surface of the fixture base 22. Four interfaces communicating with the liquid channel are provided on the upper surface of the fixture base 22. The liquid channel is connected to the inlet 131, outlet 132, sample inlet 133, and sample outlet 134 of the microfluidic chip through the four interfaces. The positions and sizes of the fixtures correspond to the positions of the inlet 131, outlet 132, sample inlet 133, and sample outlet 134 of the chip, and are of the same size. Sealing gaskets are provided at all interfaces to ensure a tight seal during liquid flow, preventing leakage. These gaskets also provide cushioning to prevent damage to the microfluidic chip from the fixture base during the closing process. The other end of the liquid channel connects to an external liquid storage device or drive pump 3. An assist hole 212 is provided at the bottom of the fixture housing 21 to help remove the fixture base 22. During use, an upward force is applied to the fixture base at the assist hole 212 to facilitate removal from the fixture housing. A connecting hole 213 is provided on the side of the fixture housing 21 to connect the liquid channel to an external liquid storage device or drive pump 3, facilitating communication between the microfluidic chip and external devices.

[0041] All drive pumps 3 are preferably Lange miniature plunger pumps, model MP500-2L-A1C2000 or MP250-2L-A1C2000, connected to the fixture base 22 via conduit 4 and steel needle, avoiding damage to the chip inlet and introduction of impurities when the steel needle is directly inserted into the microfluidic chip; the conduit is preferably PTFE tube (polytetrafluoroethylene tube). The Lange miniature plunger pump is suitable for high-precision and high-stability microfluidic sample introduction. Through precision transmission design, ultra-high precision component processing, and the use of a high-reliability photoelectric sensor for zero-point calibration, it has high full-stroke accuracy, achieving high-precision microfluidic transfer, ensuring analytical results, and reducing sample and reagent consumption, thus saving costs; to facilitate the control of the drive pumps, a plunger pump controller is configured to regulate the pressure of each channel. The controller is preferably a USBCNC Controller board (industrial standard four-axis CNC controller) to simultaneously control the four drive pumps to work in conjunction.

Claims

1. A dual "T"-shaped quantitative sampling microfluidic chip, characterized in that: The system includes a quantitative channel (121), an inlet channel (122), an outlet channel (123), an inlet channel (124), and an outlet channel (125) connected to the quantitative channel (121). The system achieves the conversion between sample storage and quantitative sample dispensing functions of the quantitative channel (121) by first adjusting the pressure difference between the outlet channel (123) and other inlet channels (122), inlet channels (124), and outlet channels (125), and then adjusting the pressure difference between the outlet channel (125) and other inlet channels (122), outlet channels (123), and inlet channels (124). When the inlet end of the quantitative channel (121) is connected to the liquid inlet channel (122), and the outlet end of the quantitative channel (121) is connected to the liquid outlet channel (123), the microfluidic chip is in the liquid inlet state, the quantitative channel (121) is used as a sample storage channel, and the sample inlet channel (124) and the sample outlet channel (125) are closed; when the inlet end of the quantitative channel (121) is connected to the sample inlet channel (124), and the outlet end of the quantitative channel (121) is connected to the sample outlet channel (125), the microfluidic chip is in the sampling state, the quantitative channel (121) is used as a quantitative sample outlet channel, and the liquid inlet channel (122) and the liquid outlet channel (123) are closed; The sample inlet channel (124) and sample outlet channel (125) are located at opposite ends of the quantitative channel (121), and the liquid inlet channel (122) and liquid outlet channel (123) are located on the side wall of the quantitative channel (121). Both the liquid inlet channel (122) and liquid outlet channel (123) are connected to the quantitative channel (121). The liquid inlet channel (122) and liquid outlet channel (123) are located on both sides of the quantitative channel (121). The liquid inlet channel (122) and the quantitative channel (121) are in a "T" shape, and the liquid outlet channel (123) and the quantitative channel (121) are in an inverted "T" shape. The inlet end of the liquid inlet channel (122) is the liquid inlet (131), the outlet end of the liquid outlet channel (123) is the liquid outlet (132), the inlet end of the sample inlet channel (124) is the sample inlet (133), and the outlet end of the sample outlet channel (125) is the sample outlet (134).

2. The dual "T"-shaped quantitative sampling microfluidic chip according to claim 1, characterized in that: The inlet channel (122) and outlet channel (123) are both arranged perpendicularly to the metering channel (121).

3. The dual "T"-shaped quantitative sampling microfluidic chip according to claim 2, characterized in that: The dual "T"-shaped quantitative sampling microfluidic chip includes a cover layer (11), a trench layer (12), and a base layer (13) arranged sequentially from top to bottom. The quantitative channel (121), liquid inlet channel (122), liquid outlet channel (123), and sample inlet channel (124) are all cavities between the liquid flow channel on the trench layer (12) and the cover layer (11). The cover layer (11) is bonded to the trench layer (12). The liquid inlet (131) and liquid outlet (132) are... The inlet (133) and outlet (134) are both located on the base layer (13). The inlet (131) is connected to the quantitative channel (121) through the inlet channel (122). The outlet (132) is connected to the quantitative channel (121) through the outlet channel (123). The inlet (133) is connected to the quantitative channel (121) through the inlet channel (124). The outlet (134) is connected to the quantitative channel (121) through the outlet channel (125).

4. A fixture for use with the dual "T"-shaped quantitative sampling microfluidic chip according to any one of claims 1-3, characterized in that: The fixture includes a fixture box (21), a fixture base (22), and a fixture cover (23). The fixture box (21) has an internal cavity. The fixture base (22) is placed inside the cavity of the fixture box (21). A microfluidic chip is placed on the fixture base (22). The fixture cover (23) is closed on the fixture box (21) and abuts against the surface of the microfluidic chip. The fixture base (22) has a liquid channel that communicates with the microfluidic chip. The upper surface of the fixture base (22) is provided with four interfaces that communicate with the liquid channel. The liquid channel communicates with the inlet (131), outlet (132), sample inlet (133), and sample outlet (134) of the microfluidic chip through the four interfaces respectively. The position and size of the interfaces of the fixture base (22) correspond to the position of the inlet (131), outlet (132), sample inlet (133), and sample outlet (134) of the microfluidic chip, and the sizes are the same. The other end of the liquid flow channel is connected to an external liquid storage device or a drive pump (3).

5. The fixture for a dual "T"-shaped quantitative sampling microfluidic chip according to claim 4, characterized in that: All interfaces are equipped with sealing gaskets.

6. The fixture for a dual "T"-shaped quantitative sampling microfluidic chip according to claim 5, characterized in that: The clamp cover (23) has an observation window (231) which is located directly above the clamp base (22). The edge of the observation window (231) is provided with a buffer gasket.

7. The fixture for a dual "T"-shaped quantitative sampling microfluidic chip according to claim 6, characterized in that: The fixture base (22) and the fixture box (21) are detachably connected. The bottom of the fixture box (21) is provided with an assist hole (212) for taking out the fixture base (22). The side wall of the fixture box (21) is provided with a communication hole (213) for connecting the liquid flow channel inside the fixture base (22) with the outside. The top edge of the fixture box (21) is provided with an arc-shaped groove (214) for picking up the microfluidic chip.

8. The fixture for a dual "T"-shaped quantitative sampling microfluidic chip according to claim 7, characterized in that: The clamp cover (23) is hinged to one side of the clamp box (21) via a hinge seat. The clamp cover (23) and the clamp box (21) are provided with a push switch on the other side of the hinge seat. The push switch includes a push latch (211) and a push tongue (232). The push latch (211) is provided on the clamp base (22), and the push tongue (232) is provided on the clamp cover (23). The push latch (211) and the push tongue (232) are arranged opposite to each other.

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