A blood smear specimen pretreatment device and method of use

Abstract

The application relates to a blood slice sample pretreatment device, which comprises a box body, a punching and liquid distribution assembly, a sample suction and drying assembly, an oscillator, a first reagent pump, a second reagent pump and a nitrogen generation assembly; the box body is connected with the nitrogen generation assembly; the punching and liquid distribution assembly, the sample suction and drying assembly, the first reagent pump and the second reagent pump are arranged in the box body; the punching and liquid distribution assembly is connected with the first reagent pump; the sample suction and drying assembly is connected with the second reagent pump; the oscillator is arranged on the punching and liquid distribution assembly and the sample suction and drying assembly. The application can realize batch punching and batch liquid adding at the same time, the box body can complete different functions in different areas, different areas can be used simultaneously or separately, different functions can be completed, personnel intervention can be reduced, errors can be reduced, and manpower can be saved.

Description

Technical Field

[0001] This invention relates to the field of medical testing technology, and in particular to a pretreatment device for blood smear specimens and its usage method. Background Technology

[0002] In recent years, with the implementation of the national two-child policy, the number of newborns has increased significantly. Based on the need for eugenics and optimal childbirth, newborn genetic metabolic disease screening, as the first step in health screening, has become increasingly widespread, leading to a gradual increase in laboratory testing of newborns. Due to the special nature of the specimen types and the requirements of the testing procedures, the specimen processing is relatively cumbersome, resulting in relatively low testing efficiency. Routine specimens are generally body fluid specimens, which can be directly processed and tested in their liquid state.

[0003] Typically, the specimen type for this project is dried blood smears, which cannot be directly processed for testing. It is necessary to first dissolve a portion of the blood on the dried blood smears into a suitable liquid solvent, and then perform processes such as extraction, drying, derivatization reaction, dissolution into a homogenized liquid, etc., before finally testing on the instrument.

[0004] Currently, in existing technologies, this process is mostly done manually, with only devices for single-hole or batch drilling to perform the drilling operation, but it is impossible to set up subsequent continuous process processing, which limits the increase in laboratory testing capabilities. Summary of the Invention

[0005] To address the aforementioned technical deficiencies in the prior art, this invention provides a pretreatment device for blood smear specimens and its usage method, which can effectively solve the problems in the background art.

[0006] To solve the above-mentioned technical problems, the technical solution provided by the present invention is as follows:

[0007] One embodiment of the present invention discloses a pretreatment device for testing blood smear specimens, including a box, a perforation and dispensing assembly, a sample aspiration and drying assembly, a shaker, a first reagent pump, a second reagent pump, and a nitrogen generating assembly; the box is connected to the nitrogen generating assembly, the perforation and dispensing assembly, the sample aspiration and drying assembly, the first reagent pump, and the second reagent pump are all disposed inside the box, the perforation and dispensing assembly is connected to the first reagent pump, the sample aspiration and drying assembly is connected to the second reagent pump, and the shaker is disposed on the perforation and dispensing assembly and the sample aspiration and drying assembly.

[0008] In any of the above embodiments, it is preferred that the interior of the box can be divided into a first region, a second region, and a third region in sequence, the first reagent pump is disposed in the first region, the perforated liquid dispensing assembly is disposed in the second region, and the sample suction and drying assembly and the second reagent pump are disposed in the third region.

[0009] In any of the above embodiments, it is preferred that the box body includes a sliding groove, a first sliding rod, a first sliding block, and a sliding rail. The sliding groove passes through the first region, the second region, and the third region. The first sliding rod passes through the first sliding block, and both ends of the first sliding rod are disposed within the sliding groove. The sliding rail is located in the second region and is longitudinally disposed on the bottom surface of the box body.

[0010] In any of the above embodiments, preferably, the perforated liquid separation assembly includes a first telescopic rod, a 96-well perforated plate, a 96-well fine needle, a sample holder, and a sample plate. One end of the first telescopic rod is fixedly connected to the first sliding block, and the other end of the first telescopic rod is fixedly connected to the top surface of the 96-well perforated plate. The 96-well fine needle is detachably connected to the bottom of the 96-well perforated plate. The sample holder is located below the 96-well perforated plate, and the bottom of the sample holder is connected to the bottom surface of the housing. The sample plate is disposed on the sample holder.

[0011] In any of the above embodiments, preferably, the perforated liquid separation assembly further includes a test tube rack, a second telescopic rod, a support plate, and a pulley. One end of the second telescopic rod is fixedly connected to the bottom of the test tube rack, and the other end of the second telescopic rod is fixedly connected to the top of the support plate. The pulley is disposed at the bottom of the support plate, wherein the pulley can cooperate with the slide rail to separate the test tube rack from the box body; the oscillator is disposed at the bottom of the support plate.

[0012] In any of the above embodiments, it is preferred that the housing further includes a second slide rod and a second slide block, the two slide rods passing through the second slide block, and the two ends of the second slide rod being disposed within the slide groove.

[0013] In any of the above embodiments, it is preferred that the sample suction and drying assembly includes a third telescopic rod, a sample suction plate, a reaction plate, and a reaction plate fixing frame. One end of the third telescopic rod is fixedly connected to the bottom of the second sliding block, and the other end of the third telescopic rod is fixedly connected to the top of the sample suction plate. The reaction plate is located below the sample suction plate and is disposed on the reaction plate fixing frame. The bottom of the sample suction plate is detachably connected to the 96-hole fine needle.

[0014] In any of the above embodiments, it is preferred that the oscillator is provided at the bottom of the reaction plate holder.

[0015] In any of the above embodiments, it is preferred that the nitrogen generating assembly includes a nitrogen generator and a nitrogen delivery pipe, one end of the nitrogen delivery pipe is connected to the housing, and the other end of the nitrogen delivery pipe is connected to the output end of the nitrogen generator.

[0016] In any of the above embodiments, preferably, the enclosure further includes a temperature control device, a temperature sensor, a controller, an indicator, a first switch door, and a second switch door. The temperature control device and the temperature sensor are disposed inside the enclosure, the control module and the indicator are disposed on the top of the enclosure, the first switch door is disposed in the second area, and the second switch door is disposed in the third area.

[0017] On the other hand, a method of using a blood smear specimen pretreatment device includes the following steps:

[0018] Open the first switch door and place the blood smear plate containing the blood smear into the sample plate;

[0019] The punching function is activated, and the blood smear is punched into the test tube in the test tube rack through a 96-well fine needle. At the same time, the first reagent is added into the test tube through the first reagent pump.

[0020] Raise and move the 96-well perforated plate to the left, remove the blood smear plate, and mix the solution in the test tube for 2 to 10 minutes.

[0021] After mixing, the solution in the test tube rack is aspirated and transferred to the reaction plate. The staff is then reminded by the indicator to remove the reaction plate, centrifuge it, return it to its original position, and dry the liquid with nitrogen.

[0022] Continue adding the second reagent to the reaction plate and shake thoroughly. React at 20–60°C for 5–15 minutes.

[0023] After the reaction is complete, the liquid is dried again by blowing with nitrogen gas for 1 to 3 minutes.

[0024] Once the process is complete, a prompt will alert staff to proceed with the next steps.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0026] This invention enables batch punching and simultaneous batch liquid addition. The chamber can be divided into zones for different functions, which can be used simultaneously or separately to perform various tasks. It also features both liquid and gas delivery systems, allowing for liquid addition and drying, ensuring reagent stability at suitable temperatures, and providing control over the entire operation with notifications upon completion. It can complete all processes for blood smear specimens except centrifugation, reducing human intervention, errors, and manpower. Compared to traditional methods, it achieves highly efficient blood smear specimen pretreatment, increasing laboratory testing capabilities by 60%. Attached Figure Description

[0027] The accompanying drawings are provided to further illustrate the present invention and, together with the embodiments thereof, are used to explain the present invention, but do not constitute a limitation thereof.

[0028] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 This is a front view of the present invention;

[0030] Figure 3 This is a side view of the first region of the housing of the present invention;

[0031] Figure 4 This is a side view of the second region of the housing of the present invention;

[0032] Figure 5 This is a side view of the third region of the housing of the present invention.

[0033] Explanation of the labels in the diagram:

[0034] Box body 1; perforated liquid separation assembly 2; sample suction and drying assembly 3; shaker 4; first reagent pump 5; second reagent pump 6; nitrogen generator assembly 7; chute 11; first slide bar 12; first sliding block 13; slide rail 14; second slide bar 15; second sliding block 16; first telescopic rod 21; 96-well perforated plate 22; 96-well fine needle 23; sample holder 24; sample plate 25; test tube rack 26; second telescopic rod 27; support plate 28; pulley 29; third telescopic rod 31; sample suction plate 32; reaction plate 33; reaction plate holder 34; nitrogen generator 71; nitrogen delivery pipe 72; temperature control device 101; temperature sensor 102; controller 103; indicator 104; first switch door 105; second switch door 106. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0036] It should be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly on or indirectly on that other component. When a component is referred to as being "connected to" another component, it can be directly connected to or indirectly connected to that other component.

[0037] In the description of this invention, it should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0038] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0039] To better understand the above technical solutions, the technical solutions of the present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0040] like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, the present invention provides a pretreatment device for testing blood smear specimens, including a housing 1, a perforated liquid dispensing assembly 2, a sample suction and drying assembly 3, a shaker 4, a first reagent pump 5, a second reagent pump 6, and a nitrogen generating assembly 7; the housing 1 is connected to the nitrogen generating assembly 7, the perforated liquid dispensing assembly 2, the sample suction and drying assembly 3, the first reagent pump 5, and the second reagent pump 6 are all disposed inside the housing 1, the perforated liquid dispensing assembly 2 is connected to the first reagent pump 5, the sample suction and drying assembly 3 is connected to the second reagent pump 6, and the shaker 4 is disposed on the perforated liquid dispensing assembly 2 and the sample suction and drying assembly 3.

[0041] like Figure 2 and Figure 4 As shown, the interior of the housing 1 can be divided into a first region, a second region, and a third region in sequence. The first reagent pump 5 is located in the first region, the perforated liquid dispensing assembly 2 is located in the second region, and the sample suction and drying assembly 3 and the second reagent pump 6 are located in the third region.

[0042] like Figure 2 and Figure 5As shown, the housing 1 includes a slide groove 11, a first slide rod 12, a first sliding block 13, and a slide rail 14. The slide groove 11 passes through the first region, the second region, and the third region. The first slide rod 12 passes through the first sliding block 13, and both ends of the first slide rod 12 are disposed within the slide groove 11, so that the first sliding block 13 can slide on the first slide rod 12. The first slide rod 12 can slide within the slide groove 11, allowing the first sliding block 13 to move laterally and longitudinally relative to the top surface of the housing 1. The slide rail 14 is located in the second region and is longitudinally disposed on the bottom surface of the housing 1.

[0043] like Figure 4 As shown, the perforated liquid separation assembly 2 includes a first telescopic rod 21, a 96-hole perforated plate 22, a 96-hole fine needle 23, a sample holder 24, and a sample plate 25. One end of the first telescopic rod 21 is fixedly connected to the first sliding block 13, and the other end of the first telescopic rod 21 is fixedly connected to the top surface of the 96-hole perforated plate 22. The 96-hole fine needle 23 is detachably connected to the bottom of the 96-hole perforated plate 22. The sample holder 24 is located below the 96-hole perforated plate 22, and the bottom of the sample holder 24 is connected to the bottom surface of the housing 1. The sample plate 25 is disposed on the sample holder 24.

[0044] In a preferred embodiment, the 96-hole perforated plate 22 is made of a high-temperature and corrosion-resistant material, and the 96-hole fine needle 23 is made of a pressure-resistant, high-temperature and corrosion-resistant material. The interior of the 96-hole fine needle 23 is hollow, and its lower end is a rotary blade. The number and diameter of the 96-hole fine needle 23 can be determined according to actual working needs, so as to realize the one-to-one perforation and sample addition of the sample on the sample plate 25. After the perforation and sample addition operations are completed, the 96-hole perforated plate 22 can be moved to the first area to facilitate the next operation.

[0045] like Figures 1-5 As shown, the perforated liquid separation assembly 2 also includes a test tube rack 26, a second telescopic rod 27, a support plate 28, and a pulley 29. One end of the second telescopic rod 27 is fixedly connected to the bottom of the test tube rack 26, and the other end of the second telescopic rod 27 is fixedly connected to the top of the support plate 28. The pulley 29 is disposed at the bottom of the support plate 28. The pulley 29 can cooperate with the slide rail 14 to separate the test tube rack 26 from the box 1.

[0046] In one specific embodiment, the first reagent pump 5 can be connected to the 96-well perforated plate 22 via a conduit. Then, through the cooperation of the slide groove 11, the first slide rod 12, and the first sliding block 13, the 96-well perforated plate 22 connected to the first sliding block 13 can move laterally and longitudinally, moving it to directly above the sample plate 25. Then, the first telescopic rod 21 moves the 96-well fine needles 23 on the 96-well perforated plate 22 downwards. The test tube rack 26 is moved upward by the second telescopic rod 27, so that the 96-well fine needle 23 can inject the blood smear from the sample plate 25 into the test tube on the test tube rack 26. At the same time, the 96-well fine needle 23 can add the first reagent loaded in the first reagent pump 5 into the test tube, realizing the batch liquid addition operation. Before and after the batch liquid addition operation is completed, the support plate 28 and the test tube rack 26 can be pulled out or put into the box 1 by the cooperation of the slide rail 14 and the pulley 29.

[0047] like Figures 1-5 As shown, the bottom of the support plate 28 is provided with the oscillator 4. In actual work, after the batch sample addition operation is completed, the oscillator 4 can be used to oscillate the first reagent and sample in the test tube to achieve full mixing and dissolution of the first reagent and sample.

[0048] like Figures 1-5 As shown, the housing 1 also includes a second sliding rod 15 and a second sliding block 16. The second sliding rod 15 passes through the second sliding block 16, and both ends of the second sliding rod 15 are disposed in the slide groove 11, so that the second sliding block 16 can slide on the second sliding rod 15 and the second sliding rod 15 can slide in the slide groove 11, so that the second sliding block 16 can move laterally and longitudinally relative to the top surface of the housing 1.

[0049] like Figures 1-5 As shown, the sample suction and drying assembly 3 includes a third telescopic rod 31, a sample suction plate 32, a reaction plate 33, and a reaction plate fixing frame 34. One end of the third telescopic rod 31 is fixedly connected to the bottom of the second sliding block 16, and the other end of the third telescopic rod 31 is fixedly connected to the top of the sample suction plate 32. The reaction plate 33 is located below the sample suction plate 32 and is mounted on the reaction plate fixing frame 34. The bottom of the sample suction plate 32 is detachably connected to the 96-hole fine needle 23.

[0050] In one specific embodiment, the second reagent pump 6 can be connected to the sample suction plate 32 via a conduit. Then, through the cooperation of the slide groove 11, the second slide rod 15, and the second sliding block 16, the sample suction plate 32 connected to the second sliding block 16 can move laterally and longitudinally, moving it directly above the sample plate 25. Then, the third telescopic rod 31 moves the 96-hole fine needle 23 on the sample suction plate 32 downwards, and the second telescopic rod 27 moves the test tube rack 26 upwards, thus achieving the desired effect on the sample suction plate. The 96-well fine needle 23 can draw the dissolved reagent from the test tube. After the drawing operation is completed, the sample plate 32 is moved to the top of the reaction plate 33 by the cooperation of the slide groove 11, the second slide rod 15 and the second sliding block 16. The sample plate 32 is then moved downward by the third telescopic rod 31 to transfer the drawn solution to the reaction plate 33 for shaking and drying. Then, the second reagent from the second reagent pump 6 is added to the reaction plate 33 again, and the shaking and drying operation is performed again.

[0051] The bottom of the reaction plate holder 34 is equipped with the shaker 4. In actual operation, after the sample aspiration operation is completed, the solution aspirated on the reaction plate 33 can be agitated by the shaker 4 to ensure that the aspirated solution can react fully.

[0052] like Figure 1 As shown, the nitrogen generating assembly 7 includes a nitrogen generator 71 and a nitrogen delivery pipe 72. One end of the nitrogen delivery pipe 72 is connected to the housing 1, and the other end of the nitrogen delivery pipe 72 is connected to the output end of the nitrogen generator 71. In actual operation, after the sample suction plate 32 transfers the solution in the test tube to the reaction plate 33, the nitrogen generator 71 can be turned on to separate and transport nitrogen from the air to the third area, thereby drying the solution on the reaction plate 33.

[0053] In another specific embodiment, one end of the nitrogen delivery pipe 72 is connected to the sample suction plate 32, and the other end of the nitrogen delivery pipe 72 is connected to the output end of the nitrogen generator 71. During the drying operation, the nitrogen gas can be vertically delivered to the top of the reaction plate 33 through the 92-hole fine needle 23 on the sample suction plate 32, so as to quickly dry the solution on the reaction plate 33.

[0054] like Figures 1-5As shown, the enclosure 1 also includes a temperature control device 101, a temperature sensor 102, a controller 103, an indicator 104, a first switch door 105, and a second switch door 106. The temperature control device 101 and the temperature sensor 102 are located inside the enclosure 1. The control module 103 and the indicator 104 are located on the top of the enclosure 1. The first switch door 105 is located in the second area, and the second switch door 106 is located in the third area.

[0055] In one specific implementation, when the experimenter is conducting an experiment, the first reagent pump 5, the second reagent pump 6, the test tube rack 26, the sample plate 25, and the reaction plate 33 can be replaced by opening the first switch door 105 and the second switch door 106, thus facilitating the experimenter's operation. The temperature sensor 102 senses the temperature value inside the chamber 1 in real time, and then the temperature control device 101 adjusts the temperature inside the chamber 1 to ensure the stability of the reagents under suitable temperature conditions, keeping the reagents within the optimal temperature range to ensure the effectiveness of the reaction. The controller 103 can perform timing, movement, and other control operations on the perforated liquid separation component 2, the sample suction and drying component 3, the shaker 4, the first reagent pump 5, the second reagent pump 6, and the nitrogen generating component 7. When the experimenter completes the operation set by the experimenter, the prompt device 104 can emit a prompt sound and flash an indicator light.

[0056] The temperature control device 101 is a common temperature control device, such as a fan or a small air conditioner, so it will not be described in detail here.

[0057] A method of using a blood smear specimen pretreatment device includes the following steps:

[0058] Open the first switch door 105 and place the blood smear plate containing the blood smear into the sample plate 25;

[0059] The punching function is activated, and the blood smear is punched into the test tube in the test tube rack 26 through the 96-hole fine needle 23. At the same time, the first reagent is added into the test tube through the first reagent pump 5.

[0060] Raise and move the 96-well perforated plate 22 to the left, remove the blood smear plate, and mix the solution in the test tube for 2 to 10 minutes.

[0061] After mixing, the solution in the test tube rack is aspirated and transferred to the reaction plate 33. The staff is reminded by the indicator 104 to remove the reaction plate 33, centrifuge it, return it to its original position, and dry the liquid with nitrogen.

[0062] Continue adding the second reagent to reaction plate 33 and shake thoroughly. React at 20–60°C for 5–15 min.

[0063] After the reaction is complete, the liquid is dried again by blowing with nitrogen gas for 1 to 3 minutes.

[0064] Once the process is complete, the staff will be reminded via the prompt device 104 to proceed with the next steps.

[0065] Compared with the prior art, the beneficial effects of the present invention are:

[0066] This invention enables batch punching and simultaneous batch liquid addition. The chamber can be divided into zones for different functions, which can be used simultaneously or separately to perform various tasks. It also features both liquid and gas delivery systems, allowing for both liquid addition and drying, ensuring reagent stability at suitable temperatures. The entire operation can be controlled, providing alerts to staff upon completion. It can complete all processes for blood smear specimens except centrifugation, reducing human intervention, errors, and manpower. Compared to traditional methods, it achieves highly efficient blood smear specimen pretreatment, enhancing laboratory testing capabilities.

[0067] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A pretreatment device for blood smear specimens, characterized in that: The assembly includes a housing (1), a perforated liquid dispensing assembly (2), a sample suction and drying assembly (3), a shaker (4), a first reagent pump (5), a second reagent pump (6), and a nitrogen generating assembly (7). The housing (1) is connected to the nitrogen generating assembly (7). The perforated liquid dispensing assembly (2), the sample suction and drying assembly (3), the first reagent pump (5), and the second reagent pump (6) are all located inside the housing (1). The perforated liquid dispensing assembly (2) is connected to the first reagent pump (5), and the sample suction and drying assembly (3) is connected to the second reagent pump (6). The shaker (4) is installed on the perforated liquid dispensing assembly (2) and the sample suction and drying assembly (3). The interior of the box (1) can be divided into a first region, a second region and a third region in sequence. The first reagent pump (5) is located in the first region, the perforated liquid dispensing assembly (2) is located in the second region, and the sample suction and drying assembly (3) and the second reagent pump (6) are located in the third region. The housing (1) includes a slide groove (11), a first slide rod (12), a first sliding block (13), and a slide rail (14). The slide groove (11) passes through the first region, the second region, and the third region. The first slide rod (12) passes through the first sliding block (13), and both ends of the first slide rod (12) are located in the slide groove (11). The slide rail (14) is located in the second region and is longitudinally arranged on the bottom surface of the housing (1). The perforated liquid separation assembly (2) includes a first telescopic rod (21), a 96-hole perforated plate (22), a 96-hole fine needle (23), a sample holder (24), and a sample plate (25). One end of the first telescopic rod (21) is fixedly connected to the first sliding block (13), and the other end of the first telescopic rod (21) is fixedly connected to the top surface of the 96-hole perforated plate (22). The 96-hole fine needle (23) is detachably connected to the bottom of the 96-hole perforated plate (22). The sample holder (24) is located below the 96-hole perforated plate (22), and the bottom of the sample holder (24) is connected to the bottom surface of the box (1). The sample plate (25) is disposed on the sample holder (24). The perforated liquid separation assembly (2) further includes a test tube rack (26), a second telescopic rod (27), a support plate (28), and a pulley (29). One end of the second telescopic rod (27) is fixedly connected to the bottom of the test tube rack (26), and the other end of the second telescopic rod (27) is fixedly connected to the top of the support plate (28). The pulley (29) is located at the bottom of the support plate (28). The pulley (29) can cooperate with the slide rail (14) to separate the test tube rack (26) from the box (1). The bottom of the support plate (28) is provided with the oscillator (4). The housing (1) further includes a second slide rod (15) and a second sliding block (16), the second slide rod (15) is inserted through the second sliding block (16), and both ends of the second slide rod (15) are located in the slide groove (11); The sample suction and drying assembly (3) includes a third telescopic rod (31), a sample suction plate (32), a reaction plate (33), and a reaction plate holder (34). One end of the third telescopic rod (31) is fixedly connected to the bottom of the second sliding block (16), and the other end of the third telescopic rod (31) is fixedly connected to the top of the sample suction plate (32). The reaction plate (33) is located below the sample suction plate (32) and is mounted on the reaction plate holder (34). The bottom of the sample suction plate (32) is detachably connected to the 96-hole fine needle (23). The 96-hole fine needle (23) is hollow inside, and its lower end is a rotary cutter.

2. The blood smear specimen pretreatment device according to claim 1, characterized in that: The bottom of the reaction plate holder (34) is provided with the oscillator (4); the nitrogen generating assembly (7) includes a nitrogen generator (71) and a nitrogen delivery pipe (72), one end of the nitrogen delivery pipe (72) is connected to the housing (1), and the other end of the nitrogen delivery pipe (72) is connected to the output end of the nitrogen generator (71).

3. The blood smear specimen pretreatment device according to claim 2, characterized in that: The enclosure (1) also includes a temperature control device (101), a temperature sensor (102), a controller (103), a prompt (104), a first switch door (105), and a second switch door (106). The temperature control device (101) and the temperature sensor (102) are located inside the enclosure (1), the controller (103) and the prompt (104) are located on the top of the enclosure (1), the first switch door (105) is located in the second area, and the second switch door (106) is located in the third area.

4. The method of using the blood smear specimen pretreatment device according to any one of claims 1 to 3, characterized in that: Includes the following steps: Open the first switch door (105) and place the blood smear plate containing the blood smear into the sample plate (25); The punching function is activated, and the blood smear is punched into the test tube in the test tube rack (26) through the 96-hole fine needle (23). At the same time, the first reagent is added into the test tube through the first reagent pump (5). Raise the 96-well perforated plate (22) and move it to the left, take out the blood smear plate, and mix the solution in the test tube for 2 to 10 minutes. After mixing, the solution in the test tube rack is aspirated and transferred to the reaction plate (33). The staff is reminded by the indicator (104) to take out the reaction plate (33), centrifuge it, put it back in its original position, and blow the liquid dry with nitrogen for 1 to 3 minutes. Continue adding the second reagent to the reaction plate (33) and shake thoroughly. React at 20-60°C for 5-15 minutes. After the reaction is complete, the liquid is dried again by blowing it with nitrogen gas. Once the process is complete, the staff will be reminded via the prompt device (104) to proceed with the next steps.

Images