Mussel mucin cream dressing and preparation method thereof

Abstract

The present application relates to the field of chemical technology, and provides a mussel mucin cream dressing and a preparation method thereof. The preparation method includes: obtaining a preset mass percentage of mussel mucin, an oily matrix, sodium hyaluronate, silk protein and a pH regulator; after dissolving the mussel mucin in purified water and heating it to 80±5°C, adding a pH regulator to the mussel mucin to adjust the pH value to a preset range; adding sodium hyaluronate and silk protein to the mussel mucin and stirring them fully, as a water phase for standby use; heating the oily matrix to 75‑90°C, as an oil phase for standby use; adding the oil phase to the water phase, stirring the oil phase and the water phase by a stirring device, until the oil phase and the water phase are evenly mixed, and obtaining a mussel mucin cream dressing of an oil-in-water system. The oil-in-water system changes the storage conditions of the product from special refrigerated or frozen storage to conventional room temperature storage, saving storage and transportation costs, and making it more convenient to use.

Description

Technical Field

[0001] The present application relates to the field of chemical technology, and in particular to a mussel mucin cream dressing and a preparation method thereof. Background Art

[0002] Mussel mucin has high application prospects due to its molecular characteristics of being rich in dopa groups and carrying a large amount of positive charges, which can effectively promote wound healing, and has strong biocompatibility and low cytotoxicity.

[0003] When mussel mucin is made into dressings and other products, it needs to be sterilized. However, mussel mucin is very active due to the dopa group in its molecule, which is easily oxidized and cross-linked, thus losing its related functions. Traditional irradiation sterilization methods will cause protein degradation or oxidation and cross-linking precipitation. Therefore, there is an urgent need for a mussel mucin dressing that can be stably sterilized and stored at room temperature. Summary of the invention

[0004] The purpose of the present application is to provide a mussel mucin cream dressing and a preparation method thereof in view of the deficiencies in the above-mentioned prior art, so as to solve the problem that the traditional sterilization process may cause the degradation or precipitation of mussel mucin.

[0005] To achieve the above objectives, the technical solutions adopted in this application are as follows:

[0006] In a first aspect, the present application provides a method for preparing a mussel mucin cream dressing, comprising:

[0007] Obtain preset mass percentages of mussel mucin, oily matrix, sodium hyaluronate, silk fibroin and a pH regulator; dissolve the mussel mucin in purified water and heat it to 80°C±5°C, then add the pH regulator to the mussel mucin to adjust the pH value to a preset range; then add sodium hyaluronate and silk fibroin to the mussel mucin and stir thoroughly to prepare the aqueous phase; heat the oily matrix to 75-90°C to prepare the oil phase; add the oil phase to the aqueous phase, stir the oil phase and the aqueous phase by a stirring device until the oil phase and the aqueous phase are evenly mixed, and obtain a mussel mucin cream dressing.

[0008] The beneficial effects of the present application are as follows: the mussel mucin cream dressing prepared by the mussel mucin cream dressing preparation method provided in the present application is an oil-in-water system cream, which can solve the problem that the dopa group contained in mussel mucin is easily oxidized. The oil-in-water system can avoid the instability and stratification of the product after traditional wet heat sterilization. The oil-in-water system can effectively avoid the degradation of protein molecules during the storage period, and the product can be stored at room temperature. The storage conditions of the product are changed from special refrigerated or frozen storage to conventional room temperature storage, saving storage and transportation costs, and being more convenient to use.

[0009] In some embodiments, the preset mass percentages include: the mass percentage of mussel mucin is 3%-8%; the mass percentage of oily matrix is ​​5%-20%; the mass percentage of sodium hyaluronate is 6%-16%; the mass percentage of silk fibroin is 1%-5%; and the remainder is purified water.

[0010] In some embodiments, after obtaining the mussel mucin cream dressing, the method further includes: filling the mussel mucin cream dressing after it drops to a preset temperature, and sterilizing the filled mussel mucin cream dressing to obtain a sterile mussel mucin cream dressing.

[0011] In some embodiments, the mussel mucin cream is an oil-in-water system cream.

[0012] In some embodiments, the preset range of pH value includes: 3.0-4.2.

[0013] In some embodiments, the oil phase and the water phase are stirred by a stirring device until the oil phase and the water phase are uniformly mixed, comprising: stirring the oil phase and the water phase by a stirring device at 1000-1500 r / min for 5-20 min.

[0014] In some embodiments, the oily base includes at least one of cetostearyl alcohol, glyceryl mono- and distearate, glyceryl stearate, beheneth-10, beheneth-25, and acrylates / C10-C30 alkyl acrylate crosspolymer.

[0015] In some embodiments, the pH adjuster includes at least one of citric acid, boric acid, acetic acid, phosphoric acid, hydrochloric acid, triethanolamine, sodium hydroxide and potassium hydroxide.

[0016] In a second aspect, the present application provides a mussel mucin cream dressing, which includes: mussel mucin, an oily matrix, sodium hyaluronate, silk fibroin, and a pH regulator.

[0017] In some embodiments, the mass percentage of mussel mucin is 3%-8%; the mass percentage of oily matrix is ​​5%-20%; the mass percentage of sodium hyaluronate is 6%-16%; the mass percentage of silk fibroin is 1%-5%; and the balance is purified water.

[0018] The beneficial effects of the second aspect can refer to the first aspect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 The mussel mucin cream dressing prepared in Example 1 of the preparation method of the mussel mucin cream dressing provided in the present application is shown;

[0021] Figure 2 The mussel mucin cream dressing prepared in Comparative Example 2 in the preparation method of the mussel mucin cream dressing provided in the present application is shown;

[0022] Figure 3 The mussel mucin cream dressing prepared in Comparative Example 5 in the preparation method of the mussel mucin cream dressing provided in the present application is shown;

[0023] Figure 4 The electrophoresis diagram of the mussel mucin cream dressing prepared by the preparation method of the mussel mucin cream dressing provided in the present application is shown. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be clearly and completely described through embodiments in combination with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all of the embodiments.

[0025] It should be noted that, unless otherwise indicated, all percentages, fractions and ratios are calculated based on the total mass of the composition of the present invention. Unless otherwise indicated, all masses of the listed ingredients are given as active substances, so they do not include solvents or by-products that may be contained in commercially available materials. The term "mass percentage content" herein can be represented by the symbol "%".

[0026] Unless otherwise indicated, all molecular weights herein are weight average molecular weight expressed in Daltons.

[0027] Unless otherwise specified, all formulations and tests herein took place at 25°C.

[0028] As used herein, "includes," "comprising," "containing," "having," or other variations are intended to encompass non-closed inclusions, and no distinction is made between these terms. The term "comprising" means that other steps and ingredients that do not affect the end result may be added. The term "comprising" also includes the terms "consisting of" and "consisting essentially of." The compositions and methods / processes of the present invention comprise, consist of, and consist essentially of the essential elements and limitations described herein, as well as any additional or optional ingredients, components, steps, or limitations described herein. As used herein, no distinction is made between the terms "efficacy," "performance," "effect," and "effect," and they will not be explained one by one below.

[0029] Dressing is a medical product that protects wounds and promotes wound healing. Among them, dressings made of mussel mucin have high application prospects because of their molecular characteristics of being rich in dopa groups and carrying a large amount of positive charges, which can effectively promote wound healing, and have strong biocompatibility and low cytotoxicity.

[0030] However, because the dopa group in its molecule is very active, it is easy to be oxidized and cross-linked, thus losing its related functions, and the protein will precipitate from the solution due to oxidative cross-linking. Traditional irradiation sterilization methods will cause protein degradation or oxidative cross-linking precipitation. Therefore, there is an urgent need for a mussel mucin dressing that can be stably sterilized and stored at room temperature.

[0031] In this regard, the present application provides a method for preparing a recombinant mussel mucin cream dressing, comprising: obtaining a preset mass percentage of mussel mucin, an oily matrix, sodium hyaluronate, silk fibroin and a pH regulator; dissolving the mussel mucin in purified water and heating it to 80°C±5°C, adding a pH regulator to the mussel mucin to adjust the pH value to a preset range; adding sodium hyaluronate and silk fibroin to the mussel mucin and stirring them thoroughly to prepare a water phase; heating the oily matrix to 75-90°C to prepare an oil phase; adding the oil phase to the water phase, stirring the oil phase and the water phase by a stirring device until the oil phase and the water phase are evenly mixed to obtain a mussel mucin cream dressing.

[0032] Among them, the oil phase matrix is ​​generally heated to 75-90°C to ensure that the oil is completely melted.

[0033] In some embodiments, the mussel mucin can be a recombinant mussel mucin or a naturally extracted one. As an example, when using recombinant mussel mucin, the recombinant mussel mucin can be a genetically recombinant fp-151 type. The fp-151 type recombinant mussel mucin is a recombinant protein composed of 6 MAP1 type (fp-1) decapeptide repeat sequences at the C-terminus and N-terminus of the MAP5 type (fp-5). To prepare the recombinant mussel mucin, the mussel mucin freeze-dried sponge can be stirred in purified water until completely dissolved and heated to 80±5°C to obtain the recombinant mussel mucin.

[0034] The mussel mucin cream dressing prepared by the mussel mucin cream dressing preparation method provided in this embodiment is an oil-in-water system cream, which can solve the problem that the dopa group contained in mussel mucin is easily oxidized. The oil-in-water system can avoid the instability and stratification of the product after traditional wet heat sterilization. The oil-in-water system can effectively avoid the degradation of protein molecules during the storage period, and the product can be stored at room temperature. The storage conditions of the product are changed from special refrigerated or frozen storage to conventional room temperature storage, saving storage and transportation costs, and being more convenient to use.

[0035] In some embodiments, the preset mass percentages include: 3%-8% of mussel mucin; 5%-20% of oily matrix; 6%-16% of sodium hyaluronate; 1%-5% of silk fibroin, and the remainder is purified water.

[0036] In some embodiments, after obtaining the mussel mucin cream dressing, the method further includes: filling the mussel mucin cream dressing after it drops to a preset temperature, and sterilizing the filled mussel mucin cream dressing to obtain a sterile mussel mucin cream dressing.

[0037] Among them, the filled mussel mucin cream dressing can be sterilized by keeping the filled mussel mucin cream dressing above 121° C. for 9-30 minutes to achieve sterilization of the mussel mucin cream dressing.

[0038] Since the mussel mucin cream dressing provided in this embodiment utilizes the amphoteric properties of mussel mucin to adopt an oil-in-water system, the oil-water stratification phenomenon that is easy to occur in traditional cream dressings during high-temperature sterilization is avoided. After the product is sterilized by wet heat, the product maintains its original state, providing a new sterilization method for sterile cream dressings.

[0039] It should be noted that the mussel mucin cream prepared by the present invention is an oil-in-water system cream. The microscopic spherical droplets formed by the oil-in-water system reduce the contact between oxygen in the air and the recombinant mussel mucin to a certain extent, preventing oxidative cross-linking. On the other hand, the microscopic spherical droplets reduce the interaction force between mussel molecules and reduce the occurrence of oxidative cross-linking between molecules, thereby effectively preventing protein cross-linking and precipitation, and increasing the stability of the protein.

[0040] In some embodiments, the preset range of pH value includes: 3.0-4.2.

[0041] In some embodiments, the pH adjuster includes at least one of citric acid, boric acid, acetic acid, phosphoric acid, hydrochloric acid, triethanolamine, sodium hydroxide and potassium hydroxide.

[0042] In some embodiments, the oil phase and the water phase are stirred by a stirring device until the oil phase and the water phase are uniformly mixed, comprising: stirring the oil phase and the water phase by a stirring device at 1000-1500 r / min for 5-20 min.

[0043] In some embodiments, the oily base includes at least one of cetostearyl alcohol, glyceryl mono- and distearate, glyceryl stearate, beheneth-10, beheneth-25, and acrylates / C10-C30 alkyl acrylate crosspolymer.

[0044] The present application also provides a mussel mucin cream dressing, which can be prepared by a preparation method of the mussel mucin cream dressing.

[0045] In some embodiments, the mussel mucin cream dressing comprises: mussel mucin, an oily base, sodium hyaluronate, silk fibroin, and a pH adjuster.

[0046] In some embodiments, the mass percentage of mussel mucin is 3%-8%; the mass percentage of oily matrix is ​​5%-20%; the mass percentage of sodium hyaluronate is 6%-16%; the mass percentage of silk fibroin is 1%-5%; and the remainder is purified water.

[0047] The mussel mucin cream dressing in this embodiment includes mussel mucin, sodium hyaluronate and silk fibroin. The network structure formed by these three substances can play a good role in drug sustained release and provide a drug administration route for oil-soluble functional raw materials.

[0048] Hereinafter, the preparation method of the mussel mucin cream dressing will be further described through examples and comparative examples.

[0049] Example 1

[0050] In this embodiment, the mass percentage of mussel mucin is 5%, the oily matrix includes 3% of mono- and distearic acid glyceryl and 5% of cetostearyl alcohol, the mass percentage of sodium hyaluronate is 10%, and the mass percentage of silk fibroin is 3%. After weighing the raw materials according to the above mass percentages, the preparation is carried out according to the following steps:

[0051] (1) A 5% freeze-dried mussel mucin sponge was stirred and dissolved completely in purified water and heated to 80°C.

[0052] (2) Add a pH adjuster to adjust the pH to 4.0.

[0053] (3) Add 10% sodium hyaluronate and 3% silk fibroin and stir thoroughly to prepare the aqueous phase for later use.

[0054] (4) Heat 3% of mono- and distearic glyceryl and 5% of cetostearyl alcohol to 80° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0055] (5) Add the oil phase to the water phase and stir at 1000 r / min for 10 min until completely mixed, then cool to room temperature for filling.

[0056] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0057] Example 2

[0058] In this embodiment, the mass percentage of mussel mucin is 8%, the oily matrix includes 20% of cetostearyl alcohol, 16% of sodium hyaluronate, and 5% of silk fibroin. After weighing the raw materials according to the above mass percentages, the preparation is carried out according to the following steps:

[0059] (1) 8% freeze-dried mussel mucin sponge was stirred and dissolved completely in purified water and heated to 85°C.

[0060] (2) Add a pH adjuster to adjust the pH to 4.2.

[0061] (3) Add 16% sodium hyaluronate and 5% silk fibroin and stir thoroughly to prepare the aqueous phase for later use.

[0062] (4) Heat 20% cetostearyl alcohol to 90° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0063] (5) Add the oil phase to the water phase and stir at 1500 r / min for 20 min until completely mixed, then cool to room temperature for filling.

[0064] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0065] Example 3

[0066] In this embodiment, the mass percentage of mussel mucin is 3%, the oily matrix includes 5% of beheneth-25, 6% of sodium hyaluronate, and 1% of silk fibroin. After weighing the raw materials according to the above mass percentages, the preparation is carried out according to the following steps:

[0067] (1) A 3% freeze-dried mussel mucin sponge was stirred and dissolved completely in purified water and heated to 75°C.

[0068] (2) Add a pH adjuster to adjust the pH to 3.0.

[0069] (3) Add 6% sodium hyaluronate and 1% silk fibroin and stir thoroughly to prepare the aqueous phase for later use.

[0070] (4) Heat 5% of beheneth-25 to 75°C until the solid oil is completely dissolved and set aside as the oil phase.

[0071] (5) Add the oil phase to the water phase and stir at 1000 r / min for 5 min until completely mixed, then cool to room temperature for filling.

[0072] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0073] Comparative Example 1

[0074] The raw materials and the mass percentages of the raw materials used in this comparative example are the same as those in Example 1, but the difference from Example 1 is that the order of adding the raw materials is adjusted, as follows:

[0075] (1) Add 10% sodium hyaluronate and 3% silk fibroin into purified water, stir to fully dissolve, and heat to 80°C.

[0076] (2) Add a pH adjuster to adjust the pH to 4.0.

[0077] (3) Add 5% freeze-dried mussel mucin sponge and stir thoroughly to prepare the aqueous phase.

[0078] (4) Heat 3% of mono- and distearic glyceryl and 5% of cetostearyl alcohol to 80° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0079] (5) Add the oil phase to the water phase and stir at 1000 r / min for 10 min until completely mixed, then cool to room temperature for filling.

[0080] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0081] Comparative Example 2

[0082] In this comparative example, the addition ratio of the raw materials is not within the mass percentage range provided in this application, and is as follows:

[0083] The mass percentage of mussel mucin is 2%, the oily matrix includes 1% of mono- and distearic acid glyceryl and 2% of cetostearyl alcohol, the mass percentage of sodium hyaluronate is 3%, and the mass percentage of silk fibroin is 0.5%. After weighing the raw materials according to the above mass percentages, the preparation is carried out according to the following steps:

[0084] (1) A 2% freeze-dried mussel mucin sponge was stirred and dissolved completely in purified water and heated to 80°C.

[0085] (2) Add a pH adjuster to adjust the pH to 6.0.

[0086] (3) Add 3% sodium hyaluronate and 0.5% silk fibroin and stir thoroughly to prepare the aqueous phase for later use.

[0087] (4) Heat 1% of mono- and distearic glyceryl and 2% of cetostearyl alcohol to 80° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0088] (5) Add the oil phase to the water phase and stir at 1000 r / min for 10 min until completely mixed, then cool to room temperature for filling.

[0089] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0090] Comparative Example 3

[0091] The difference between this comparative example and Example 1 is that the raw materials used do not include silk fibroin, and the mass percentages of the remaining raw materials are the same as those in Example 1, specifically as follows:

[0092] Weigh the raw material ingredients according to the proportion.

[0093] (1) A 5% freeze-dried mussel mucin sponge was stirred and dissolved completely in purified water and heated to 80°C.

[0094] (2) Add a pH adjuster to adjust the pH to 4.0.

[0095] (3) Add 10% sodium hyaluronate and stir thoroughly to set aside as the aqueous phase.

[0096] (4) Heat 3% of mono- and distearic glyceryl and 5% of cetostearyl alcohol to 80° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0097] (5) Add the oil phase to the water phase and stir at 1000 r / min for 10 min until completely mixed, then cool to room temperature for filling.

[0098] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0099] Comparative Example 4

[0100] The difference between this comparative example and Example 1 is that the raw materials used do not include sodium hyaluronate, and the mass percentages of the remaining raw materials are the same as those in Example 1, specifically as follows:

[0101] Weigh the raw material ingredients according to the proportion.

[0102] (1) A 5% freeze-dried mussel mucin sponge was stirred and dissolved completely in purified water and heated to 80°C.

[0103] (2) Add a pH adjuster to adjust the pH to 4.0.

[0104] (3) Add 3% silk fibroin and stir thoroughly to set aside as the aqueous phase.

[0105] (4) Heat 3% of mono- and distearic glyceryl and 5% of cetostearyl alcohol to 80° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0106] (5) Add the oil phase to the water phase and stir at 1000 r / min for 10 min until completely mixed, then cool to room temperature for filling.

[0107] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0108] Comparative Example 5

[0109] The difference between this comparative example and Example 1 is that in the preparation process, only the oil phase is heated, the water phase is prepared at room temperature, and the mass percentages of the remaining raw materials and the process are the same as those in Example 1, as follows:

[0110] (1) Dissolve 5% freeze-dried mussel mucin sponge in purified water at room temperature by stirring.

[0111] (2) Add a pH adjuster to adjust the pH to 4.0.

[0112] (3) Add 10% sodium hyaluronate and 3% silk fibroin and stir thoroughly to prepare the aqueous phase for later use.

[0113] (4) Heat 3% of mono- and distearic glyceryl and 5% of cetostearyl alcohol to 80° C. until the solid fat is completely dissolved and set aside as the oil phase.

[0114] (5) Add the oil phase to the water phase and stir at 1000 r / min for 10 min until completely mixed, then cool to room temperature for filling.

[0115] (6) After filling, the product is sterilized by wet heat sterilization in a high-pressure steam sterilizer to obtain a sterile recombinant mussel mucin cream dressing.

[0116] The results showed that when the water phase was prepared at room temperature and the oil phase was added, the oil phase would partially solidify, and the resulting cream would have uneven ingredients and solid particles, which was not conducive to product stability.

[0117] Figure 1 The mussel mucin cream dressing prepared in Example 1 of the preparation method of the mussel mucin cream dressing provided in the present application is shown. Figure 2 The mussel mucin cream dressing prepared in Comparative Example 2 in the preparation method of the mussel mucin cream dressing provided in the present application is shown. Figure 3 The mussel mucin cream dressing prepared in Comparative Example 5 in the preparation method of the mussel mucin cream dressing provided in the present application is shown.

[0118] according to Figure 1 , Figure 2 and Figure 3 It can be determined that when the mass percentage of the raw materials in the preparation method of the mussel mucin cream dressing is outside the range provided in the present application, or is not heated according to the preset temperature, a formed mussel mucin cream dressing cannot be obtained.

[0119] The above-mentioned examples and comparative examples were subjected to stability tests to observe whether there was stratification after sterilization of examples 1, 2, 3 and comparative examples 1, 2, 3, 4, 5 at 3000 r / min for 30 minutes, stratification or anti-coarsening at 50°C for 24 hours and at -18°C for 24 hours. The results are shown in Table 1:

[0120] Table 1

[0121]

[0122] Figure 4 The electrophoresis diagram of the mussel mucin cream dressing prepared by the preparation method of the mussel mucin cream dressing provided in the present application is shown.

[0123] Take the mussel mucin cream dressing prepared in Example 1 as an example. The mussel mucin cream dressing was divided into 6 portions, one of which was not sterilized, and the other five portions were sterilized by moist heat, 5K irradiation, 10K irradiation, 15K irradiation and 20K irradiation, respectively. Then, an electrophoresis test was performed.

[0124] refer to Figure 4 ,Depend on Figure 4 It can be seen that the protein bands after moist heat sterilization are almost the same as before sterilization, indicating that the moist heat sterilized protein has not been significantly degraded. However, after different doses of irradiation sterilization, the protein is completely degraded and loses its function.

[0125] The present invention provides a sterile product that can protect protein stability, ensure the integrity of protein molecules, promote wound healing, and bring good news to patients after medical aesthetics.

[0126] In the above embodiments, the description of each embodiment has its own emphasis. For parts that are not described or recorded in detail in a certain embodiment, reference can be made to the relevant descriptions of other embodiments.

[0127] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. These modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present application, and should all be included in the protection scope of the present application.

Claims

1. A method for preparing a mussel mucin cream dressing, It is characterized in that The method comprises: Obtaining preset mass percentages of mussel mucin, oily matrix, sodium hyaluronate, silk fibroin, and pH regulator; After dissolving the mussel mucin in purified water and heating to 80±5° C., a pH regulator is added to the mussel mucin to adjust the pH value to 3.0-4.2; Adding sodium hyaluronate and silk fibroin to the mussel mucin and stirring thoroughly to prepare the aqueous phase for later use, wherein the network structure formed by the mussel mucin, the sodium hyaluronate and the silk fibroin can play a good role in sustained drug release and provide a drug administration route for the oil-soluble functional raw materials; Heat the oily base to 75-90°C and set aside as the oil phase; adding the oil phase to the water phase, and stirring the oil phase and the water phase by a stirring device until the oil phase and the water phase are uniformly mixed to obtain a mussel mucin cream dressing; Wherein, the preset mass percentage includes: The mass percentage of the mussel mucin is 3%-8%; The mass percentage of the oily matrix is ​​5%-20%; The mass percentage of the sodium hyaluronate is 6%-16%; The mass percentage of the silk fibroin is 1%-5%; The balance is purified water; After obtaining the mussel mucin cream dressing, the method further comprises: After the mussel mucin cream dressing has dropped to a preset temperature, it is filled, and the filled mussel mucin cream dressing is sterilized to obtain a sterile mussel mucin cream dressing; The oily base includes at least one of cetostearyl alcohol, glyceryl mono- and distearate, glyceryl stearate, beheneth-10, beheneth-25, and acrylates / C10-C30 alkyl acrylate crosspolymer.

2. The method according to claim 1, It is characterized in that The mussel mucin cream is an oil-in-water system cream.

3. The method according to claim 1, It is characterized in that The oil phase and the water phase are stirred by a stirring device until the oil phase and the water phase are uniformly mixed, comprising: The oil phase and the water phase are stirred at 1000-1500 r / min for 5-20 min by a stirring device.

4. The method according to claim 1, It is characterized in that The pH adjuster includes at least one of citric acid, boric acid, acetic acid, phosphoric acid, hydrochloric acid, triethanolamine, sodium hydroxide and potassium hydroxide.

5. A mussel mucin cream dressing, It is characterized in that The mussel mucin cream dressing is prepared by the preparation method of the mussel mucin cream dressing according to claim 1, and the mussel mucin cream dressing comprises: Mussel mucin, oily base, sodium hyaluronate, silk fibroin and pH adjuster.

6. The mussel mucin cream dressing according to claim 5, It is characterized in that The mass percentage of the mussel mucin is 3%-8%; The mass percentage of the oily matrix is ​​5%-20%; The mass percentage of the sodium hyaluronate is 6%-16%; The mass percentage of the silk fibroin is 1%-5%; The balance is purified water.

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