A Cr(VI) reductase and its preparation method and application in the restoration of chromium-contaminated water bodies

By isolating the Cr(VI) reductase nfnB from Asgard Archae and expressing it in E. coli, the problem of low Cr(VI) reduction efficiency in the prior art is solved, and efficient and rapid repair of chromium-contaminated water bodies is achieved. Suitable for industrial applications.

CN116200351BActive Publication Date: 2025-01-07HUAZHONG AGRI UNIV
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Patent Information

Application Number
CN202211223747.4
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-10-08
Publication Date
2025-01-07
Estimated Expiration
2042-10-08

AI Technical Summary

Technical Problem

The prior art has low reduction efficiency of Cr(VI) in chromium-contaminated water bodies, which cannot meet industrial needs, resulting in unstable repair effects and long cycles.

Method used

An efficient Cr(VI) reductase nfnB was isolated from Asgard Archae. By constructing a recombinant plasmid and expressing it in E. coli, combined with optimized amino acid sequence and purification methods, a high Cr(VI) reduction was prepared. Active enzymes are suitable for rapid reduction of Cr(VI) to Cr(III) over a wide temperature range.

Benefits of technology

The maximum reduction rate of this enzyme is 69.2μmol·min-1·mg-1, which is significantly higher than that of the existing enzymes. It can quickly and effectively remove Cr(VI) contamination under natural environmental conditions and is suitable for the repair of chromium-contaminated water bodies.

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Abstract

The invention discloses a Cr(VI) reductase and a preparation method thereof and an application thereof in the restoration of chromium-contaminated water bodies. The amino acid sequence of the Cr(VI) reductase is shown in SEQ ID NO.1. The preparation method is as follows: a nucleotide sequence such as a fragment shown in SEQ ID NO.3 is connected to a plasmid to obtain a recombinant plasmid, and the recombinant plasmid is then transformed into Escherichia coli BL21 (DE3) to obtain a recombinant bacterium. The recombinant bacterium is induced to express by IPTG, and after the expression is completed, the cells are collected and crushed, and purified. After the Cr(VI) reductase is added to the Cr(VI) contaminated water body, it can quickly reduce the highly biologically toxic and highly water-soluble Cr(VI) into the Cr(III) with low biological toxicity and low water solubility, thereby achieving a good restoration effect.
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Citation Information

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