Magnetic stirrer assembly

Abstract

The application discloses a kind of empty suspension type magnetic stirring assembly to solve the problem of insufficient sealing effect of driving mode in the field of cell culture or biopharmaceutical, comprising: sealed shell assembly;First magnetic rotating part, in the sealed shell assembly;Second magnetic rotating part, in the sealed shell assembly outside, second magnetic rotating part is equipped with stirring paddle;Driving assembly, connect the first magnetic rotating part and can drive the first magnetic rotating part rotation, the first magnetic rotating part rotation can drive the second magnetic rotating part rotation.The power component and transmission component of stirring part are better isolated from the space in the liquid storage container by the magnetic force of the first magnetic rotating part to drive the second magnetic rotating part, avoid the problem of insufficient sealing effect of existing mechanical driving mode.

Description

Suspended magnetic stirring unit Technical Field

[0001] This invention relates to the fields of cell culture or biopharmaceuticals, and more particularly to a suspended magnetic stirring assembly. Background Technology

[0002] Cell culture is a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce, and maintain their main structures and functions.

[0003] A bioreactor is a production experimental device that provides a reaction environment for experiments and production such as cell culture, pharmaceutical production, animal and plant tissue culture, microbial fermentation production, biological product preparation, and wastewater treatment. It is sometimes also called a fermenter or reactor.

[0004] With the continuous development of cell culture technology, the widespread application of bioreactors has become a major trend in the cell culture and pharmaceutical fields. As the demand for safety, convenience, and efficiency in cell culture processes continues to increase, single-use systems have been used for more than 30 years. In China, although the application of single-use systems started relatively late, it has developed rapidly in recent years. A number of biopharmaceutical companies have introduced single-use systems into their pharmaceutical processes. Currently, single-use systems in the pharmaceutical field are widely used in cell culture / fermentation, preparation and storage of culture media and buffers, as well as in the storage and transfer of process materials, final product formulation, freeze-thaw cycles of bulk solutions and semi-finished products, filling, sampling, and other pharmaceutical processes.

[0005] Currently, commonly used disposable bioreactors typically employ mechanical seals to seal the components of the stirring assembly relative to the container. This sealing method is prone to leakage of the culture medium inside the container, thereby affecting the normal use of the reactor and failing to effectively control the impact of cultured cells on the external environment, such as the risk of bacterial contamination. Summary of the Invention

[0006] The present invention provides a bioreactor that aims to at least solve one of the technical problems existing in the prior art.

[0007] The technical solution of the present invention provides a suspended magnetic stirring assembly, comprising:

[0008] Sealed housing assembly;

[0009] The first magnetic rotating component is located inside the sealed housing assembly;

[0010] The second magnetic rotating component is located outside the sealed housing assembly, and the second magnetic rotating component is provided with stirring blades;

[0011] The driving component is connected to the first magnetic rotating component and can drive the first magnetic rotating component to rotate, and the rotation of the first magnetic rotating component can drive the second magnetic rotating component to rotate.

[0012] Furthermore, the sealed housing assembly includes:

[0013] first tube body;

[0014] The mounting housing is connected to the second end of the first tube. The first magnetic rotating component is located inside the mounting housing, and the second magnetic rotating component is sleeved on the first tube or the mounting housing.

[0015] Furthermore, the driving components include:

[0016] The drive shaft is located in the middle of the first tube, and the second end of the drive shaft is inserted into the mounting housing and connected to the first magnetic rotating component.

[0017] Furthermore, the suspended magnetic stirring assembly also includes:

[0018] The second tube is located in the middle of the first tube, and the drive shaft is located in the middle of the second tube.

[0019] Furthermore, the driving components also include:

[0020] Mounting plate, the first end of the second tube is connected to the mounting plate by a thread, and the second end of the second tube is connected to the mounting housing by a thread;

[0021] The drive unit is mounted on the mounting plate. The first end of the drive shaft passes through the mounting plate and is connected to the drive unit. The drive unit can drive the drive shaft to rotate.

[0022] Furthermore, the first end of the drive shaft is provided with a connecting sleeve, and the output end of the drive component is connected to the connecting sleeve by a key connection.

[0023] Furthermore, the first tube is a silicone tube;

[0024] The first end of the mounting housing includes a mounting tube, and the second magnetic rotating component is sleeved on the mounting tube.

[0025] The sealed housing assembly also includes:

[0026] The intermediate connecting tube has its first end connected to the second end of the silicone tube, and its second end connected to the installation tube.

[0027] Furthermore, the first end of the intermediate connecting tube is provided with an annular protrusion, which can be fastened into the second end of the silicone tube;

[0028] The second end of the intermediate connecting pipe is connected to the installation pipe via a thread.

[0029] Furthermore, the first magnetic rotating component includes:

[0030] The first rotating housing is located inside the mounting housing;

[0031] The first magnetic component is disposed inside the first rotating housing;

[0032] The first end of the first rotating housing is connected to the inner wall of the first end of the mounting housing via a bearing.

[0033] The second end of the first rotating housing is connected to the inner wall of the second end of the mounting housing via a bearing.

[0034] Furthermore, the second magnetic rotating component includes:

[0035] The second rotating housing is fitted onto the mounting housing;

[0036] The second magnetic component is disposed inside the second rotating housing;

[0037] The second rotating housing is connected to the mounting housing via a bearing.

[0038] The beneficial effects of this invention are as follows:

[0039] The suspended magnetic stirring assembly of the present invention drives the second magnetic rotating component by the magnetic force of the first magnetic rotating component. The power component and transmission component of the stirring part are better isolated from the space inside the liquid storage container, thus avoiding the problem of insufficient sealing effect in the existing mechanical driving method.

[0040] The suspended magnetic stirring assembly is easy to install, eliminating the need for the traditional through-type drive shaft. The liquid storage container is easy to fold, facilitating the packaging, transportation, and on-site installation of the bioreactor. The materials used to isolate the inside and outside of the storage container can be metal-free, avoiding the negative impact of metal on the inside of the bioreactor and making it easier for the bioreactor to achieve a sterile environment.

[0041] Multiple suspended magnetic stirring components can be flexibly installed on the storage container according to needs, making it easier to adapt to bioreactors of different capacities and providing better stirring effect, sterile environment and sealing in large-capacity bioreactors such as 6000L. Attached Figure Description

[0042] Figure 1 is a cross-sectional view 1 of a embodiment of the suspended magnetic stirring assembly;

[0043] Figure 2 is a cross-sectional view 2 of the embodiment of the suspended magnetic stirring assembly;

[0044] Figure 3 is an enlarged structural diagram of part A in Figure 3.

[0045] Reference numerals: First tube body 210, top plate 220, mounting plate 230, mounting housing 300, mounting tube section 310, first magnetic rotating component 400, drive shaft 510, connecting sleeve 520, second magnetic rotating component 600, stirring blade 700, second tube body 800, intermediate connecting tube 910, annular buckle 920. Detailed Implementation

[0046] The following will describe several embodiments of the present invention, including embodiments corresponding to the accompanying drawings. It should be understood that the drawings are used to assist in understanding the technical features and technical solutions of the present invention, and should not be construed as limiting the scope of protection of the present invention.

[0047] The following will provide a clear and complete description of the concept, specific structure, and technical effects of the present invention in conjunction with the embodiments and accompanying drawings, so as to fully understand the purpose, solution, and effects of the present invention. It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other.

[0048] It should be noted that, unless otherwise explicitly defined, when a feature is referred to as "fixed," "connected," or "installed" on another feature, it can be directly fixed or connected to the other feature, or it can be indirectly fixed or connected to the other feature. The terms "fixed," "connected," and "installed" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this invention in conjunction with the specific content of the technical solution.

[0049] It should be noted that the descriptions of orientations or positional relationships indicated by terms such as up, down, left, right, top, bottom, front, back, inside, outside, first end, and second end used in this invention are based on the orientations or positional relationships indicated by the accompanying drawings or embodiments. They are only for the purpose of facilitating the description of this invention and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0050] It should be noted that the term "and / or" as used in this invention includes any combination of one or more of the related listed items, "several" means one or more, "multiple" means at least two, "greater than", "less than", "exceeding" are understood to exclude the number itself, and "above", "below", "within" are understood to include the number itself.

[0051] It should be noted that the self-rotation defined in this invention refers to the ability of a rotating component to rotate around its own axis, which can be driven to rotate by other power components or transmission components.

[0052] It should be noted that the use of "first" and "second" in this invention is only for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of technical features or the order of the technical features.

[0053] It should be noted that, unless otherwise expressly defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used in this specification is for the purpose of describing particular embodiments only and not for limiting the invention.

[0054] A basic embodiment of the present invention provides a suspended magnetic stirring assembly, as shown in Figures 1-3, comprising:

[0055] Sealed housing assembly;

[0056] The first magnetic rotating component is located inside the sealed housing assembly;

[0057] The second magnetic rotating component is located outside the sealed housing assembly, and the second magnetic rotating component is provided with stirring blades;

[0058] The driving component is connected to the first magnetic rotating component and can drive the first magnetic rotating component to rotate, and the rotation of the first magnetic rotating component can drive the second magnetic rotating component to rotate.

[0059] The sealed housing assembly can be considered as a higher-level concept including a first tube, an intermediate connecting tube, and a mounting housing. It can have a first magnetic rotating component inside and a second magnetic rotating component outside, separating the two magnetic rotating components. A drive assembly, which can be considered as a higher-level concept including a drive shaft, a drive component, and a mounting plate, drives the first magnetic rotating component to rotate. The rotation of the first magnetic rotating component drives the rotation of the second magnetic rotating component, thus achieving isolated installation of the second magnetic rotating component and the drive assembly. This allows the second magnetic rotating component to normally stir the culture medium in the storage container, while the second magnetic rotating component and the drive assembly do not affect the interior of the storage container due to the isolation effect of the sealed housing assembly.

[0060] The present invention also provides a bioreactor, referring to Figures 1-3, comprising a liquid storage container and a suspended magnetic stirring assembly; wherein the suspended magnetic stirring assembly includes:

[0061] The first tube 210 is disposed inside the liquid storage container. The first end of the first tube 210 is connected to the inner wall of the liquid storage container. Furthermore, both the first tube 210 and the liquid storage container can be configured as deformable structures. The liquid storage container can be made of a high-barrier multilayer co-extruded film conforming to pharmaceutical standards and heat-sealed. The liquid storage container can be folded for easy transportation and installation.

[0062] The mounting housing 300 is connected to the second end of the first tube 210. The mounting housing 300 is used to protect the first magnetic rotating component 400. Furthermore, the mounting housing 300 may include two parts: one part is used to house the first magnetic rotating component 400, and the other part is used to connect the first tube 210 and mount the second magnetic rotating component 600.

[0063] The first magnetic rotating component 400 is disposed inside the mounting housing 300;

[0064] The drive shaft 510 is located in the middle of the first tube 210. The second end of the drive shaft 510 is inserted into the mounting housing 300 and connected to the first magnetic rotating component 400. It can be understood that the middle part is not limited to the middle position. It can be understood that the middle part is all or part of the area between the two ends. The second end of the drive shaft 510 can be connected to the first magnetic rotating component 400 through a common transmission connection method such as key connection or threaded connection.

[0065] The second magnetic rotating component 600 is sleeved on the first tube 210 or the mounting housing 300. The second magnetic rotating component 600 is provided with a stirring blade 700. Furthermore, the stirring blade 700 can be integrally formed with the housing of the second magnetic rotating component 600, or it can be set on the second magnetic rotating component 600 through a common mounting and fixing structure. The second magnetic rotating component 600 can be located on the axial side of the first magnetic rotating component 400 or on the radial side of the first magnetic rotating component 400.

[0066] The rotation of the drive shaft 510 can drive the first magnetic rotating component 400 to rotate, and the rotation of the first magnetic rotating component 400 can drive the second magnetic rotating component 600 to rotate.

[0067] In the basic embodiment, the suspended magnetic stirring assembly drives the first magnetic rotating component 400 to rotate via the drive shaft 510, and uses the magnetic effect of the first magnetic rotating component 400 to drive the second magnetic rotating component 600 to rotate. The first tube 210, in conjunction with the mounting shell 300, isolates the drive shaft 510 from the inner cavity of the storage container, avoiding the risk of contamination caused by traditional mechanically driven stirring and the influence of the metal parts of the drive unit on the inside of the storage container. This improves the aseptic assurance capability of the bioreactor, and the storage container can be easily folded and packaged, and can easily meet the requirements of irradiation sterilization.

[0068] By using the structure of the suspended magnetic stirring assembly in the basic embodiment, the drive shaft 510 does not need to be a long stirring shaft, allowing the liquid storage container to be folded for transport. The installation process can be completed after the components inside the liquid storage container, such as the first tube 210, the mounting housing 300, the first magnetic rotating component 400, and the second magnetic rotating component 600, are installed. After ensuring the sealing of the liquid storage container, the drive shaft 510 is then inserted into the first tube 210, completing the transmission connection between the drive shaft 510 and the first magnetic rotating component 400.

[0069] Utilizing magnetically driven impellers, this technology can accommodate biological cell cultures of various capacities, minimizing cell damage under low-shear conditions while achieving high homogenization at low speeds. In particular, the 6000L bioreactor can culture more cells within the same timeframe.

[0070] It is understandable that "suspended" in "suspended magnetic stirring assembly" can be understood as the magnetic stirring assembly being suspended inside the liquid storage container.

[0071] Extending the basic embodiment, referring to Figures 1-3, the suspended magnetic stirring assembly further includes:

[0072] The second tube 800 is located in the middle of the first tube 210, and the drive shaft 510 is located in the middle of the second tube 800. It can be understood that the middle part is not limited to the middle position, but can be understood as all or part of the area between the two ends. The setting of the second tube 800 is convenient for protecting the drive shaft 510 and also convenient for filling the gap between the drive shaft 510 and the first tube 210.

[0073] Further extensions of the basic embodiment, referring to Figures 1-3, the first tube 210 is a silicone tube, and the second tube 800 is a stainless steel tube. The silicone tube facilitates the folding of the liquid storage container, and silicone is also a preferred material for isolating the liquid storage container and the drive shaft 510. The stainless steel tube allows the soft silicone tube to be stretched as much as possible before installing the drive shaft 510, making the installation and positioning of the drive shaft 510 more accurate and facilitating the connection of the first magnetic rotating component 400.

[0074] It is understandable that the first tube can also be made of other soft materials that meet the needs of cell culture, and the second tube can also be made of other rigid materials that meet the needs of cell culture.

[0075] A further extension of the basic embodiment, referring to Figures 1-3, includes the suspended magnetic stirring assembly further comprising:

[0076] Mounting plate 230 is located outside the liquid storage container;

[0077] The first end of the second tube 800 is connected to the mounting plate 230 by a thread, and the second end of the second tube 800 is connected to the mounting housing 300 by a thread. The second tube 800 can be better positioned by fixing the drive shaft 510 by the thread at both ends. Considering the deformability of the silicone tube, limiting the inner diameter of the second tube 800 to be slightly larger than the shaft diameter of the drive shaft 510 can effectively limit the installation space of the drive shaft 510, which facilitates the connection between the drive shaft 510 and the first magnetic rotating component 400.

[0078] Understandably, the two ends of the second tube can also be connected to the mounting plate and the mounting housing using common fixing methods such as snap-fit.

[0079] Extending the basic embodiment, referring to Figures 1-3, the suspended magnetic stirring assembly further includes:

[0080] The driving component is located outside the liquid storage container and is mounted on the mounting plate 230. Specifically, the driving component is mounted on the first end of the mounting plate 230. The first end of the driving shaft 510 passes through the mounting plate 230 and is connected to the driving component. Specifically, the first end of the driving shaft 510 passes through the liquid storage container and the mounting plate 230 and is connected to the driving component. The driving component can drive the driving shaft 510 to rotate. The driving component can be a motor, specifically a geared motor. The driving component can be installed on the outer wall of the liquid storage container using common installation methods such as threaded connections. The setting of the driving component facilitates driving the driving shaft 510 to rotate outside the liquid storage container, thereby driving the first magnetic rotating component 400 and the second magnetic rotating component 600 to rotate. The external setting of the driving component facilitates installation.

[0081] A further extension of the basic embodiment, referring to Figures 2-3, is that the first end of the drive shaft 510 is provided with a connecting sleeve 520. The output end of the drive component can be connected to the connecting sleeve 520 by a key connection. The setting of the connecting sleeve 520 facilitates the transmission connection between the drive shaft 510 and the drive component. The second end of the connecting sleeve 520 is fixed to the first end of the drive shaft 510. The first end of the connecting sleeve 520 is provided with a groove. A keyway can be provided in the groove. The output shaft of the drive component can be inserted into the groove. The key on the output shaft cooperates with the keyway.

[0082] It is understandable that the drive components and drive shafts can also be connected using other common transmission connection methods.

[0083] As an extension of the basic embodiment, referring to FIG3, the first end of the first tube 210 is provided with a top plate 220. The top plate 220 is connected to the inner wall of the liquid storage container by heat fusion. The top plate 220 facilitates fixing the first tube 210 on the liquid storage container and also facilitates the sealing connection between the first end of the first tube 210 and the inner wall of the liquid storage container.

[0084] It is understandable that the wall of the liquid storage container is located between the top plate and the mounting plate, and other common sealing connection methods can also be used between the top plate and the inner wall of the liquid storage container.

[0085] It is understandable that the top plate at the first end of the first tube body is sealed to the inner wall of the liquid storage container, the second end of the first tube body is sealed to the first end of the intermediate connecting tube, the second end of the intermediate connecting tube is sealed to the mounting tube section at the first end of the mounting housing, and the first tube body, intermediate connecting tube, and mounting housing cooperate to isolate the inner cavity of the liquid storage container from the mounting space of the second tube body and the drive shaft.

[0086] An extension of the basic embodiment, referring to Figures 1-3, the first tube 210 is a silicone tube;

[0087] The first end of the mounting housing 300 includes a mounting tube 310, which is connected to the second end of the silicone tube. The second magnetic rotating member 600 is sleeved on the mounting tube 310. The mounting tube 310 facilitates the connection of the silicone tube and also facilitates the installation of the second magnetic rotating member 600.

[0088] A further extension of the basic embodiment, referring to Figures 1-3, includes the suspended magnetic stirring assembly further comprising:

[0089] The intermediate connecting tube 910 has its first end connected to the second end of the silicone tube, and its second end connected to the mounting tube 310. The intermediate connecting tube 910 facilitates the sealing connection between the silicone tube and the mounting tube 310, thus ensuring a tight seal.

[0090] As a further extension of the basic embodiment, referring to Figures 1-3, the first end of the intermediate connecting tube 910 is provided with an annular protrusion 920, which can be fastened into the second end of the silicone tube. The annular protrusion 920 is installed into the second end of the silicone tube by compression, thereby achieving a sealed connection.

[0091] The second end of the intermediate connecting pipe 910 is connected to the installation pipe 310 by a thread to achieve a sealed connection.

[0092] Understandably, the two ends of the intermediate connecting tube can also be connected to the silicone tube and the installation tube by ultrasonic welding, adding sealing rings, or other common sealing connection methods.

[0093] It is understandable that the radial outer wall of the mounting tube can be provided with an annular groove for mounting the second magnetic rotating component, or the space between the stepped surface on the mounting tube and the second end of the intermediate connecting tube can be used to mount the second magnetic rotating component.

[0094] An extension of the basic embodiment, referring to Figures 1-3, the first magnetic rotating member 400 includes:

[0095] The first rotating housing is connected to the drive shaft 510. The axis of the first rotating housing can be provided with a threaded hole or keyway to accommodate the second end of the drive shaft 510.

[0096] A first magnetic element is disposed within a first rotating housing. The inner cavity of the first rotating housing can be configured as an annular shape, and the shape of the first magnetic element is correspondingly an annular. The first rotating housing can protect the first magnetic element.

[0097] It is understandable that the first rotating housing can be configured as a two-part housing with a cover connection, which facilitates the installation of the first magnetic component inside.

[0098] Understandably, the first magnetic rotating component can also be formed directly from a magnetic component.

[0099] A further extension of the basic embodiment, referring to Figures 1-3, is that the first end of the first rotating housing is connected to the inner wall of the first end of the mounting housing 300 via a bearing;

[0100] The second end of the first rotating housing is connected to the inner wall of the second end of the mounting housing 300 via a bearing. The bearing connection allows the first rotating housing to rotate more stably relative to the mounting housing 300, and the two sets of bearings cooperate to ensure that the first rotating housing does not move axially during rotation.

[0101] It is understandable that the outer wall of the first rotating housing and the inner wall of the mounting housing can be provided with stepped surfaces or grooves to accommodate bearing installation.

[0102] An extension of the basic embodiment, referring to Figures 1-3, the second magnetic rotating member 600 includes:

[0103] The second rotating housing is fitted onto the mounting housing 300;

[0104] A second magnetic component is disposed within a second rotating housing. The inner cavity of the second rotating housing can be annular, and the shape of the second magnetic component is correspondingly annular. The radial cross-section of the second magnetic component can be U-shaped. The inner wall of the first end of the second rotating housing can be provided with annular protrusions that can be inserted into the second magnetic component. The second rotating housing can protect the second magnetic component.

[0105] Understandably, the second rotating housing can be configured as a two-part housing that fits together, facilitating the installation of a second magnetic component inside.

[0106] Understandably, the second magnetic rotating component can also be formed directly from a magnetic component.

[0107] It is understandable that magnetic components can be general magnetic elements, specifically magnets.

[0108] A further extension of the basic embodiment, referring to Figures 1-3, involves connecting the second rotating housing to the mounting housing 300 via a bearing connection. This bearing-based connection allows the second rotating housing to rotate more stably relative to the mounting housing 300.

[0109] It is understandable that the outer wall of the second rotating housing and the inner wall of the mounting housing can be provided with stepped surfaces or grooves to accommodate bearing installation.

[0110] An extension of the basic embodiment, referring to Figures 1-3, the first end of the drive shaft 510 extends outside the liquid storage container. The first end of the drive shaft 510 is connected to the outer wall of the liquid storage container via a bearing. This bearing connection allows the drive shaft 510 to rotate more stably relative to the liquid storage container. A boss can be provided on the outer wall of the liquid storage container that accommodates the drive shaft 510. The first end of the drive shaft 510 passes through the boss, and an annular groove can be provided at the first end of the boss. The bearing can be disposed within the annular groove, and both ends of the bearing are connected to the drive shaft 510 and the inner wall of the annular groove, respectively.

[0111] As an extension of the basic embodiment, referring to Figure 1, both the upper and lower ends of the liquid storage container are equipped with suspended magnetic stirring components. The two sets of suspended magnetic stirring components can stir the culture medium in the liquid storage container at multiple positions, enabling better cell culture. This configuration is particularly effective for large-capacity bioreactors.

[0112] Understandably, this suspended magnetic stirring assembly is easy to install and can be flexibly installed at multiple locations on the storage container as needed, not limited to the first and second ends of the storage container. It is especially widely used in large-capacity bioreactors such as 6000L, resulting in better cell culture effects. Furthermore, even when multiple suspended magnetic stirring assemblies are installed, the sealing and sterility protection capabilities of the storage container are not affected. It eliminates the need for the traditional long stirring shaft structure, and is also very convenient for transportation and installation.

[0113] An extension of the basic embodiment, the liquid storage container is a deformable liquid storage bag. The liquid storage bag can be made of a high-barrier multilayer co-extruded film that meets pharmaceutical standards and is heat-sealed. It is easy to fold and transport, and also easy to install. It can be used as a disposable bioreactor, eliminating the cleaning work after the first culture is completed. The installation position is flexible and the cost is low.

[0114] In addition to the basic embodiment, the bioreactor also includes:

[0115] The inlet pipe assembly connects to the first end of the liquid storage container;

[0116] The drain pipe assembly connects to the second end of the liquid storage container;

[0117] An air inlet pipe assembly, connecting the first and / or second ends of a liquid storage container;

[0118] An exhaust pipe assembly, connected to the first and / or second end of a liquid storage container;

[0119] Sampling tube assembly, connected to the second end of the liquid storage container;

[0120] A bubble generator is located inside the liquid storage container and connected to the air inlet on the liquid storage container.

[0121] The liquid inlet assembly may include a feeding assembly, one end of which is connected to the feeding port of the liquid storage container, and the other end is connected to an external feeding device. The liquid inlet assembly may also include a replenishment assembly, one end of which is connected to the feeding port of the liquid storage container, and the other end is connected to an external replenishment device. The liquid inlet assembly is used to input materials into the liquid storage container as needed.

[0122] One end of the drain pipe assembly is connected to the drain port of the storage container, and the other end is connected to an external harvesting device. The drain pipe assembly can be used to drain waste liquid or to harvest culture medium.

[0123] The intake pipe assembly and the exhaust pipe assembly together form a venting pipe assembly, which is used to supply or exhaust air into the liquid storage container as needed.

[0124] One end of the sampling tube assembly is connected to the sampling port of the liquid storage container, and the other end is connected to an external sampling device. The sampling tube assembly is used to take samples from the liquid storage container.

[0125] A bubble generator is used to generate bubbles in a liquid storage container.

[0126] A sterilization filter can be installed in the venting pipe assembly to clean the gas passing through the storage container.

[0127] The inlet tube assembly, outlet tube assembly, aeration tube assembly, and sampling tube assembly work together to provide the environment required for cell culture.

[0128] The suspended magnetic stirring assembly of the present invention can be installed by the following method, including the following steps:

[0129] The first magnetic rotating component and the second magnetic rotating component are installed on the mounting housing, and the stirring blade is installed on the second magnetic rotating component;

[0130] The first end of the first tube is installed on the inner wall of the liquid storage container, the second end of the first tube is installed on the first end of the intermediate connecting tube, and the second end of the intermediate connecting tube is installed on the first end of the mounting shell.

[0131] The first tube, the intermediate connecting tube, and the mounting shell are installed into the liquid storage container. After the liquid storage container is folded, packaged, and transported to the designated installation position, the liquid storage container is unfolded.

[0132] Insert the second tube into the first tube, and install the second end of the second tube onto the first end of the mounting housing;

[0133] Insert the drive shaft into the second tube and connect the second end of the drive shaft to the first magnetic rotating component.

[0134] Install the mounting plate on the first end of the second tube, install the drive component on the first end of the mounting plate, and pass the first end of the drive shaft through the mounting plate and connect it to the drive component for transmission.

[0135] The above installation method enables the folding and transportation of the liquid storage container, reducing transportation difficulty and improving transportation efficiency. The installation of the first tube, intermediate connecting tube, and mounting shell ensures that the inner cavity of the liquid storage container is isolated from the installation space of the second tube and drive shaft, achieving a better sealing effect. The subsequent installation of the second tube, drive shaft, drive components, and other parts will not affect the inside of the liquid storage container. This avoids the risk of bacterial contamination caused by traditional mechanically driven stirring blades and the impact of metal parts of the drive section on the inside of the liquid storage container, improving the aseptic assurance capability of the bioreactor. The liquid storage container can be easily folded and packaged and easily meets the requirements of irradiation sterilization.

[0136] It should be noted that in this specification, terms such as "one embodiment", "some embodiments", "basic embodiment", and "extended embodiment" may be used to describe several embodiments of the present invention, and the specific features, structures, materials or characteristics in the several embodiments may be combined in accordance with the principles and spirit of the present invention.

[0137] Although some embodiments of the present invention have been shown and described in this specification, the present invention should not be limited to the above embodiments. As long as they achieve the technical effects of the present invention by the same or equivalent means, any changes, modifications, equivalent substitutions and equivalent variations of these embodiments within the spirit and principles of this disclosure, without departing from the principles and purpose of the present invention, should be included within the scope of protection of this disclosure and should be considered to fall within the protection scope of the present invention.

Claims

1. A suspended magnetic stirring assembly, characterized in that, include: Sealed housing assembly; The first magnetic rotating component (400) is disposed within the sealed housing assembly; A second magnetic rotating component (600) is disposed outside the sealed housing assembly, and a stirring blade (700) is provided on the second magnetic rotating component (600); a driving assembly is connected to the first magnetic rotating component (400) and is capable of driving the first magnetic rotating component (400) to rotate, and the rotation of the first magnetic rotating component (400) can drive the second magnetic rotating component (600) to rotate; the sealed housing assembly includes a first tube (210), a mounting housing (300) and an intermediate connecting pipe (910), the first tube (210) is a silicone tube, the mounting housing (300) is connected to the second end of the first tube (210), the first end of the mounting housing (300) includes a mounting tube portion (310), the first magnetic rotating component (400) is disposed inside the mounting housing (300), the second magnetic rotating component (600) is sleeved on the mounting tube portion (310), and the intermediate connecting pipe (910) The first end of the intermediate connecting tube (910) is connected to the second end of the silicone tube, and the second end of the intermediate connecting tube (910) is connected to the mounting tube (310). The driving assembly includes a driving shaft (510), the middle part of which is located inside the first tube body (210), and the second end of the driving shaft (510) is inserted into the mounting housing (300) and connected to the first magnetic rotating member (400). The assembly also includes a second tube body (800), the middle part of which is located inside the first tube body (210), and the middle part of the driving shaft (510) is located inside the second tube body (800).

2. The suspended magnetic stirring assembly according to claim 1, characterized in that: The drive assembly further includes: a mounting plate (230), the first end of the second tube (800) being threadedly connected to the mounting plate (230), and the second end of the second tube (800) being threadedly connected to the mounting housing (300); a drive member, mounted on the mounting plate (230), the first end of the drive shaft (510) passing through the mounting plate (230) and connected to the drive member, the drive member being capable of driving the drive shaft (510) to rotate.

3. The suspended magnetic stirring assembly according to claim 2, characterized in that: The first end of the drive shaft (510) is provided with a connecting sleeve (520), and the output end of the drive component is connected to the connecting sleeve (520) by a key connection.

4. The suspended magnetic stirring assembly according to claim 1, characterized in that: The first end of the intermediate connecting tube (910) is provided with an annular protrusion (920), which can be fastened into the second end of the silicone tube; the second end of the intermediate connecting tube (910) is connected to the mounting tube (310) by means of a thread.

5. The suspended magnetic stirring assembly according to claim 1, characterized in that: The first magnetic rotating component (400) includes: a first rotating housing disposed within the mounting housing (300); a first magnetic component disposed within the first rotating housing; a first end of the first rotating housing connected to the inner wall of the first end of the mounting housing (300) via a bearing; and a second end of the first rotating housing connected to the inner wall of the second end of the mounting housing (300) via a bearing.

6. The suspended magnetic stirring assembly according to claim 1, characterized in that: The second magnetic rotating component (600) includes: a second rotating housing, sleeved on the mounting housing (300); a second magnetic component, disposed inside the second rotating housing; and the second rotating housing is connected to the mounting housing (300) via a bearing.

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