A parasitic wasp attractant for removing tea tree pests such as tea moth and its preparation method

Abstract

The invention discloses a parasitic wasp attractant for tea tree pest removal tea moth, and the parasitic wasp attractant comprises the following raw materials in parts by weight, 5-10 parts of jasmone, 8-15 parts of (E)-3-hexenol, 3-8 parts of 3-octanone, 5-10 parts of leaf acetate, 3-10 parts of 6-methyl-5-heptene-2-one, 5-15 parts of (E)-β-farnesene, 8-20 parts of linalool, 5-12 parts of 2,6-dimethyl-3,7-octadiene-2,6-diol, 8-18 parts of carrageenan, and 15-30 parts of water. The invention belongs to the technical field of parasitic wasp attractants, a parasitic wasp attractant for tea tree pest removal tea moth with no side effects, good economic performance, and harmless, and a preparation method thereof, which can realize the ability to attract multiple types of parasitic wasps while attracting the natural enemy of the tea moth, the parasitic wasp Microgastrinae Choeras sp. Using attractants in late May and replacing the bait core every 15 days can significantly reduce the number of tea moth larvae in late July of the same year and reduce the use of chemical pesticides to control tea moth.

Description

Technical Field

[0001] The invention belongs to the technical field of parasitic wasp attractants, and in particular relates to a parasitic wasp attractant for eliminating the tea moth, a tea tree pest, and a preparation method thereof. Background Art

[0002] In nature, to defend against herbivorous insects, pest plants release pest-induced volatile compounds as a "help" signal to lure natural enemies, thereby indirectly defending against pests. Tea trees are important economic crops and are susceptible to a variety of pests during their growth. Long-term reliance on chemical control will lead to excessive pesticide residues in tea leaves, and green prevention and control methods must be developed. For example, the small green leafhopper is one of the most serious pests in tea areas, and the leafhopper trilobite is a parasitic wasp on its eggs. It has a high parasitic rate on leafhopper eggs laid in tender stems and a significant follow-up effect, which can effectively defend against the small green leafhopper.

[0003] Traditional parasitic wasp attractants are a mixture of various volatiles. Although they can have an attractant effect, they will affect the tea tree's own volatiles' ability to attract parasitic wasps, and will affect the growth of tea leaves themselves. As an economic product of tea trees, the taste of tea leaves itself directly affects the taste of the product. With the development of scientific planting concepts, people can extract volatiles from natural plants to make attractants. For this reason, we propose to use attractants made from tea tree volatiles as the main ingredient, so as to improve the attractant effect on parasitic wasps and achieve better pest control without affecting the tea trees themselves.

[0004] The tea moth (Agriophara rhombata Meyr.) is a serious pest in my country's tea gardens. It usually occurs from April to October each year and has strong resistance to pesticides. In severe outbreak years, tea production can be reduced by more than 50%. After investigation and analysis, it was found that the choeras.sp. subfamily is the natural enemy of the tea moth. It usually parasitizes the body of the tea moth during its larval stage, which can reduce the feeding ability of the tea moth larvae and thus reduce the insect population. The use of attractants can effectively gather parasitic wasps at a distance to achieve the effect of killing insects. Summary of the invention

[0005] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a parasitic wasp attractant for tea tree pest control, the tea moth, which has no side effects, good economic performance and is harmless, and a preparation method thereof, which can improve the ability to attract parasitic wasps without affecting the growth of the tea tree itself, and will not affect the changes in the taste of the tea produced by the tea tree.

[0006] The technical scheme adopted by the present invention is as follows: a parasitic wasp attractant for tea tree pest control tea moth, comprising the following volatile substances in parts by weight: 5-10 parts of jasmone, 8-15 parts of (E)-3-hexenol, 3-8 parts of 3-octanone, 5-10 parts of leaf acetate, 3-10 parts of 6-methyl-5-heptene-2-one, 5-15 parts of (E)-β-farnesene, 8-20 parts of linalool, 5-12 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 8-18 parts of carrageenan, and 15-30 parts of water.

[0007] The preferred weight ratio of the parasitic wasp attractant for tea tree pest control tea moth is: 7-8 parts of jasmone, 10-12 parts of (E)-3-hexenol, 5-7 parts of 3-octanone, 6-8 parts of leaf acetate, 5-8 parts of 6-methyl-5-heptene-2-one, 8-12 parts of (E)-β-farnesene, 12-15 parts of linalool, 7-9 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 10-15 parts of carrageenan and 18-26 parts of water.

[0008] The optimal weight ratio of the parasitic wasp attractant for tea moth for eliminating tea tree pests is: 7 parts of jasmone, 11 parts of (E)-3-hexenol, 6 parts of 3-octanone, 7 parts of leaf acetate, 6 parts of 6-methyl-5-heptene-2-one, 10 parts of (E)-β-farnesene, 13 parts of linalool, 8 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 12 parts of carrageenan and 22 parts of water.

[0009] The preparation method of the parasitic wasp attractant for tea tree pest control of tea moth is as follows:

[0010] Step 1: Take 7 parts of jasmone, 11 parts of (E)-3-hexenol, 6 parts of 3-octanone, 7 parts of leaf acetate, 6 parts of 6-methyl-5-heptene-2-one, 10 parts of (E)-β-farnesene, 13 parts of linalool, 8 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 12 parts of carrageenan, and 22 parts of water according to the weight ratio for later use.

[0011] Step 2: Mix (E)-3-hexenol and 3-octanone evenly using a centrifugal mixer;

[0012] Step 3: mixing leaf acetate and 6-methyl-5-heptene-2-one;

[0013] Step 4: adding part of water to the mixture of leaf acetate and 6-methyl-5-heptene-2-one, and stirring to mix;

[0014] Step 5: Add carrageenan to the remaining water and stir thoroughly;

[0015] Step 6: adding the mixture of (E)-3-hexenol and 3-octanone prepared in step 1 to the water and carrageenan solvent prepared in step 5;

[0016] Step 7: adding the remaining jasmone, (E)-β-farnesene, linalool, and 2,6-dimethyl-3,7-octadiene)-2,6-diol to the mixture obtained in step 6 in sequence;

[0017] Step 8: Add the mixture of leaf acetate, 6-methyl-5-heptene-2-one and water prepared in step 4 into the mixture prepared in step 7, and mix thoroughly.

[0018] Furthermore, the concentration of the mixture of leaf acetate and 6-methyl-5-heptene-2-one described in step 4 after mixing with water is 40%.

[0019] Furthermore, the above steps are all prepared at room temperature.

[0020] After adopting the above technical scheme, the beneficial effects of the present invention are as follows: the present invention is a parasitic wasp attractant for tea tree pest control tea moth, the composition of volatiles is complex and the release amount is large, it can effectively induce parasitic wasps and locate hosts, and can enable parasitic wasps to search for tea moth larvae more promptly and accurately. The main component of the preparation is the volatiles of the tea tree itself, which can effectively reduce the influence of the volatiles on the smell and taste of the tea itself, and avoid the problem of reduced tea quality due to the attractant. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 The figure is a graph showing the number of parasitic wasps attracted by the three attractants in this embodiment and their differences;

[0022] Figure 2 These are some of the parasitized tea moth larvae and parasitic wasp cocoons picked in this example. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0024] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0025] Embodiment 1: different ratios of attractant configuration

[0026] Attractant 1 preparation: 5 parts of jasmone, 8 parts of (E)-3-hexenol, 3 parts of 3-octanone, 5 parts of leaf acetate, 3 parts of 6-methyl-5-heptene-2-one, 5 parts of (E)-β-farnesene, 8 parts of linalool, 5 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 8 parts of carrageenan, and 15 parts of water;

[0027] Attractant 2 preparation: 10 parts of jasmone, 15 parts of (E)-3-hexenol, 8 parts of 3-octanone, 10 parts of phytol acetate, 10 parts of 6-methyl-5-heptene-2-one, 15 parts of (E)-β-farnesene, 20 parts of linalool, 12 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 18 parts of carrageenan, and 30 parts of water;

[0028] Attractant 3 preparation: 7 parts of jasmone, 11 parts of (E)-3-hexenol, 6 parts of 3-octanone, 7 parts of leaf acetate, 6 parts of 6-methyl-5-heptene-2-one, 10 parts of (E)-β-farnesene, 13 parts of linalool, 8 parts of 2,6-dimethyl-3,7-octadiene)-2,6-diol, 12 parts of carrageenan, and 22 parts of water;

[0029] When used specifically, the parasitic wasp attractant for tea tree pest control of tea moth of the present invention needs to be prepared with a stirring mixer, and the specific preparation steps are as follows:

[0030] Step 1: Mix (E)-3-hexenol and 3-octanone evenly using a centrifugal mixer;

[0031] Step 2: mixing leaf acetate and 6-methyl-5-heptene-2-one;

[0032] Step 3: adding part of water to the mixture of leaf acetate and 6-methyl-5-heptene-2-one, and stirring to mix;

[0033] Step 4: Add carrageenan to the remaining water and stir thoroughly;

[0034] Step 5: adding the mixture of (E)-3-hexenol and 3-octanone prepared in step 1 to the water and carrageenan solvent prepared in step 5;

[0035] Step 6: adding the remaining jasmone, (E)-β-farnesene, linalool, and 2,6-dimethyl-3,7-octadiene)-2,6-diol to the mixture obtained in step 6 in sequence;

[0036] Step 7: Add the mixture of leaf acetate, 6-methyl-5-heptene-2-one and water prepared in step 4 into the mixture prepared in step 7, and mix thoroughly.

[0037] Example 2: Field test of attracting parasitic wasps

[0038] Experimental method: Pure water was used as the control group, and parasitic wasp attractants were configured according to attractants 1, 2, and 3 as the experimental group. 200 μL of n-hexane and each ratio of attractant were respectively placed in the middle groove of an eraser head to make an experimental lure core, which was stored at 4°C for later use. On May 18, 2022, a terraced tea garden of the same variety was selected in Menghai County, Yunnan Province, and the prepared rubber heads were placed on a white sticky board (25cm×30cm), and the sticky board was placed horizontally 20cm above the tea shoots. There were 4 groups in total, including the experimental group and the control group, with 10 replicates in each group, and a total of 40 sticky boards were placed, with a spacing of 10 meters between each board. The number of parasitic wasps on the sticky boards was counted after 5 days.

[0039] Results and analysis: Figure 1 Compared with the control, the three attractants all had significant effects on attracting parasitic wasps in tea gardens. The three attractants attracted the same species of parasitic wasps, and a total of three parasitic wasps were attracted. The sequencing analysis of the COI gene using the universal primer showed that the three parasitic wasps were Pimpla aequalis, Choeras sp., and Brachymeria lasus. It can be determined that the three attractant ratios can attract Choeras sp., so they can be used as attractants for parasitic wasps, the natural enemies of tea moths.

[0040] Example 3: Tea moth population statistics

[0041] Experimental method: Pure water was used as the control group, and parasitic wasp attractants were configured according to attractants 1, 2, and 3 as the experimental group. 200 μL of n-hexane and each ratio of attractant were respectively taken and placed in the middle groove of an eraser head to make an experimental lure core, which was stored at 4°C for later use. On May 19, 2022, in order to ensure that the experimental groups did not affect each other, four terrace tea gardens with the same planting variety of Example 2 and no significant difference in the distribution density of tea moth larvae in Menghai County, Yunnan Province were selected. Four plots were taken from each tea field to investigate the distribution density of tea moth larvae in the experimental field. Each plot was 1m2. The prepared rubber heads were placed 20 cm above the tea shoots, and the lure core was replaced every 15 days. According to the occurrence law of the pest, the distribution of tea moth larvae was investigated again after 60 days, and the groups were compared.

[0042] Results and analysis: As shown in Table 1, there was no significant difference in the number of tea moth larvae per square meter in the four tea fields before the experiment (P>0.05). After 60 days, the distribution density of tea moth larvae was investigated. The number of tea moth larvae in the tea gardens where the three parasitic wasp attractants were placed decreased significantly (P<0.05). There was no significant difference among the three ratios of attractants, and Figure 2 These are some of the bee cocoons parasitized by the larvae of the tea moth collected from the tea garden. It can be determined that the three parasitic wasp attractants can significantly reduce the number of tea moth larvae.

[0043] Table 1 Distribution of tea moth larvae per unit area before and after the experiment

[0044] Grouping Comparison Attractant 1 Attractant 2 Attractant 3 The number of larvae before the test 55±7a 49±9a 54±6a 56±7a Number of larvae after 60 days 32±8a 19±3b 22±5b 17±6b

[0045] Note: Values ​​with different lowercase letters in the same row are significantly different from each other.

[0046] Although the embodiments of the present invention have been shown and described, it is understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents. In short, if those skilled in the art are inspired by them and design structural modes and embodiments similar to the technical solution without creativity without departing from the purpose of the present invention, they shall all fall within the scope of protection of the present invention.

Claims

1. A parasitic wasp attractant for tea tree pest control, characterized in that: The invention comprises the following components in parts by weight: 5-10 parts of jasmone, 8-15 parts of (E)-3-hexenol, 3-8 parts of 3-octanone, 5-10 parts of leaf acetate, 3-10 parts of 6-methyl-5-heptene-2-one, 5-15 parts of (E)-β-farnesene, 8-20 parts of linalool, 5-12 parts of 2,6-dimethyl-3,7-octadiene-2,6-diol, 8-18 parts of carrageenan and 15-30 parts of water.

2. The parasitic wasp attractant for tea tree pest control of tea moth according to claim 1, characterized in that: 7-8 parts of jasmone, 10-12 parts of (E)-3-hexenol, 5-7 parts of 3-octanone, 6-8 parts of leaf acetate, 5-8 parts of 6-methyl-5-heptene-2-one, 8-12 parts of (E)-β-farnesene, 12-15 parts of linalool, 7-9 parts of 2,6-dimethyl-3,7-octadiene-2,6-diol, 10-15 parts of carrageenan and 18-26 parts of water.

3. The parasitic wasp attractant for tea tree pest control of tea moth according to claim 1, characterized in that: 7 parts of jasmone, 11 parts of (E)-3-hexenol, 6 parts of 3-octanone, 7 parts of phytol acetate, 6 parts of 6-methyl-5-heptene-2-one, 10 parts of (E)-β-farnesene, 13 parts of linalool, 8 parts of 2,6-dimethyl-3,7-octadiene-2,6-diol, 12 parts of carrageenan and 22 parts of water.

4. A method for preparing a parasitic wasp attractant for tea tree pest control of tea moth as claimed in claim 3, characterized in that: The steps include: Step 1: Prepare according to the weight ratio; Step 2: Mix (E)-3-hexenol and 3-octanone evenly using a centrifugal mixer; Step 3: mixing leaf acetate and 6-methyl-5-heptene-2-one; Step 4: adding part of water to the mixture of leaf acetate and 6-methyl-5-heptene-2-one, and stirring and mixing, wherein the concentration of the mixture of leaf acetate and 6-methyl-5-heptene-2-one after mixing with water is 40%; Step 5: Add carrageenan to the remaining water and stir thoroughly; Step 6: adding the mixture of (E)-3-hexenol and 3-octanone prepared in step 1 to the water and carrageenan solvent prepared in step 5; Step 7: adding the remaining jasmone, (E)-β-farnesene, linalool, and 2,6-dimethyl-3,7-octadiene-2,6-diol to the mixture obtained in step 6 in sequence; Step 8: Add the mixture of leaf acetate, 6-methyl-5-heptene-2-one and water prepared in step 4 into the mixture prepared in step 7, and mix thoroughly.

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