Biological product extractor

Abstract

This invention discloses a biological product extractor, belonging to the technical field of nucleic acid extraction equipment. The biological product extractor includes a support unit and a horizontally adjustable eccentric vibration module. The support unit includes, from top to bottom, an electromagnet disk, a temperature regulating element, and a first moving plate. The electromagnet disk is used to hold a type plate containing magnetic beads. The electromagnet disk is electrically connected to a control module to control its magnetic phase. The temperature regulating element is used to regulate the temperature of the type plate above the electromagnet disk. The output end of the horizontally adjustable eccentric vibration module is connected to the first moving plate to shake the type plate above the electromagnet disk. This invention combines the functions of a magnetic field module for regulating the aggregation and dispersion of magnetic beads, vibration and shaking, and temperature regulation. It avoids the addition and removal of biological products between multiple devices, reducing cross-contamination of biological products and improving the efficiency and quality of biological product preparation.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid extraction equipment technology, and in particular to a biological product extractor. Background Technology

[0002] Magnetic bead-based nucleic acid extraction is a novel nucleic acid extraction technology that uses nano-biological magnetic beads as carriers. Nucleic acid molecules can specifically recognize and bind to the silanol groups on the surface of the magnetic beads, and aggregate or disperse under the influence of an external magnetic field. This completely eliminates the manual processes of centrifugation and supernatant extraction in traditional nucleic acid extraction, thus achieving automated nucleic acid extraction. It is widely used in various fields such as clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, and molecular biology research.

[0003] The magnetic bead method for nucleic acid extraction generally includes four main steps: lysis, binding, washing, and elution. Each step can be achieved individually or in combination by various different methods. The cell lysis buffer in the magnetic bead method is a protein denaturant that can lyse the cells of animals, plants, and pathogenic microorganisms and denature the proteins bound to nucleic acids, releasing the nucleic acids. The magnetic beads can specifically adsorb nucleic acids. Through washing, impurities such as proteins other than nucleic acids are removed. Then, the nucleic acids adsorbed on the magnetic beads are dissociated by the elution buffer to obtain a nucleic acid solution with high purity and concentration. This nucleic acid solution can be used for PCR amplification, enzyme digestion, molecular hybridization, etc.

[0004] However, the entire process of obtaining nucleic acid products by vibrating, rinsing, and cleaning magnetic beads requires many steps and is undertaken by complex mechanical structures and multiple modules in the automation process. This not only poses a risk of biological cross-contamination but also reduces the efficiency of biological product preparation.

[0005] Therefore, there is an urgent need to provide a biological product extraction instrument to solve the above problems. Summary of the Invention

[0006] The purpose of this invention is to provide a biological product extractor that combines the functions of a magnetic field module for adjusting the dispersion of magnetic beads, vibration and shaking, and temperature regulation. This avoids the addition and removal of biological products between multiple devices, thereby reducing cross-contamination of biological products and improving the efficiency and quality of biological product preparation.

[0007] To achieve the above objectives, the following technical solution is provided:

[0008] Biological product extraction instrument, including:

[0009] The support unit includes an electromagnet disk, a temperature regulating element, and a first moving plate arranged sequentially from top to bottom. The electromagnet disk is used to place a type plate containing magnetic beads. The electromagnet disk is electrically connected to a control module to control the magnetic phase of the electromagnet disk itself. The temperature regulating element is used to regulate the temperature of the type plate above the electromagnet disk.

[0010] A horizontally adjustable eccentric vibration module, the output end of which is connected to the first moving plate, is used to shake the type plate above the electromagnet disk.

[0011] As an optional solution for the bioproduct extractor, the horizontally adjustable eccentric vibration module includes a motor and an eccentric shaft arranged in a vertical direction. The output shaft of the motor is fixedly provided with a mounting block. The mounting block has a first strip-shaped hole extending radially. The upper end of the eccentric shaft is rotatably connected to the first moving plate, and the lower end of the eccentric shaft is connected to the first strip-shaped hole. The axis of the eccentric shaft is not coaxial with the axis of the output shaft of the motor.

[0012] As an optional solution for the bioproduct extractor, the side wall of the mounting block is provided with a second strip hole, which communicates with the first strip hole, and a fastener passes through the second strip hole and is connected to the lower end of the eccentric shaft.

[0013] As an optional solution for the bioproduct extractor, a bearing pressure plate is installed on the output shaft of the motor, and a threaded hole is provided on the mounting block for installing a counterweight.

[0014] As an optional solution for the biological product extractor, the motor housing is provided with an organic plate, the upper surface of the organic plate is provided with a first linear guide rail extending in a first direction, the bearing unit also includes a second movable plate, the lower end face of the second movable plate is slidably disposed on the first linear guide rail, the upper end face of the second movable plate is provided with a second linear guide rail extending in a second direction, and the lower end face of the first movable plate is slidably disposed on the second linear guide rail.

[0015] As an optional solution for the biological product extractor, the upper surface of the plate is provided with two first linear guide rails that are parallel and spaced apart, and the upper end surface of the second moving plate is provided with two second linear guide rails that are parallel and spaced apart.

[0016] As an alternative to the bioproduct extractor, the second moving plate is provided with a clearance hole for the output shaft of the motor to pass through.

[0017] As an optional solution for the bioproduct extractor, a base plate is also included, on which the bottom of the motor is mounted. A circuit board module is mounted on the base plate and is electrically connected to the temperature regulating element.

[0018] As an optional solution for the bioproduct extraction instrument, a heat insulation plate is provided between the temperature regulating element and the first moving plate.

[0019] As an optional solution for the bioproduct extraction instrument, the plate types include 96-well shallow plate, 24-well plate, 48-well plate and 96-well deep plate.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] The bioproduct extractor provided by this invention places a type plate containing magnetic beads and culture solution on an electromagnet disk. The electromagnet disk can adjust the magnetic phase, which is reflected in the adsorption and release of magnetic beads. This allows for both enrichment and dispersion of magnetic beads and matrix-style control, enabling modulation of the magnetic phase for different separation types of magnetic beads, thus accommodating multiple type plates. In terms of bioproducts, this means removing non-specific deposits in different culture solutions, then enriching them onto magnetic beads for further cleaning, until the cleaning step is complete, at which point the bioproducts are released from the magnetic beads, achieving solid-liquid separation.

[0022] The biological product extractor provided by this invention has an output end of a horizontally adjustable eccentric vibration module connected to the first moving plate. By adjusting the amplitude and vibration frequency in both directions, it can be compatible with various types of plates and shake the type of plate above the electromagnet disk, so that the culture solution is fully and uniformly mixed, without the need for any external machinery.

[0023] The biological product extractor provided by this invention has a temperature control element that enables rapid heating and cooling at different times, achieving efficient temperature changes with temporal resolution. Combined with magnetic bead rinsing and temperature increase, it allows biological products (nucleic acid-DNA) to be efficiently separated from the magnetic beads, thereby improving recovery efficiency.

[0024] In summary, the biological product extractor provided by this invention has the functions of adjusting the magnetic field module for the polymerization and dispersion of magnetic beads, vibration and shaking, and temperature regulation. It avoids the addition and removal of biological products between multiple devices, which not only reduces cross-contamination of biological products, but also improves the preparation efficiency and quality of biological products. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the content of the embodiments of the present invention and these drawings without creative effort.

[0026] Figure 1 This is a front view of the biological product extraction instrument in an embodiment of the present invention;

[0027] Figure 2 for Figure 1 A magnified view of a section at point A in the middle;

[0028] Figure 3 This is a cross-sectional view of the biological product extraction instrument in an embodiment of the present invention;

[0029] Figure 4 This is an explosion diagram of the biological product extractor in an embodiment of the present invention;

[0030] Figure 5 This is a schematic diagram of the assembly of the motor, eccentric shaft and dynamic balance counterweight in an embodiment of the present invention.

[0031] Figure label:

[0032] 1. Electromagnetic disc; 2. Temperature regulating element; 3. First moving plate; 4. Type plate; 5. Motor; 6. Eccentric shaft; 7. Mounting block; 71. First strip hole; 72. Second strip hole; 73. Threaded hole; 8. Fastener; 9. Bearing pressure plate; 10. Machine plate; 11. First linear guide rail; 12. Second moving plate; 121. Clearance hole; 13. Second linear guide rail; 14. Base plate; 15. Circuit board module; 16. Heat insulation plate; 17. First slider; 18. Second slider; 19. Bearing. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0034] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.

[0035] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0036] In the description of this invention, it should be noted that the terms "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship commonly used when the product of this invention is in use. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance. In the description of this invention, unless otherwise stated, "a plurality of" means two or more.

[0037] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0038] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0039] Embodiments of the present invention are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0040] In the development of automation in life sciences, magnetic bead extraction, as an essential DNA / RNA method for cell sorting, protein purification, and nucleic acid enrichment, is widely used in automated equipment for the extraction of all life products and is a necessary and core automation module in this field.

[0041] However, the entire process of vibrating, rinsing, cleaning, and obtaining nucleic acid products from magnetic beads requires many steps, and the automation process is handled by complex mechanical structures and multiple modules, so the throughput and preparation efficiency remain unresolved. There is a trade-off between the efficiency of the preparation process and the quality of the biological products (proteins, nucleic acids, cells) obtained.

[0042] To avoid the addition and removal of biological products across multiple devices, thereby reducing cross-contamination and improving the efficiency and quality of biological product preparation, this embodiment provides a biological product extractor, which is described below in conjunction with... Figures 1 to 5 The specific details of this embodiment are described in detail below. In this embodiment, the bioproduct extractor includes a support unit and a horizontally adjustable eccentric vibration module. The support unit includes, from top to bottom, an electromagnet disk 1, a temperature regulating element 2, and a first moving plate 3. The electromagnet disk 1 is used to hold a type plate 4 containing magnetic beads. The electromagnet disk 1 is electrically connected to a control module to control its own magnetic phase. The temperature regulating element 2 is used to regulate the temperature of the type plate 4 above the electromagnet disk 1. The output end of the horizontally adjustable eccentric vibration module is connected to the first moving plate 3 to shake the type plate 4 above the electromagnet disk 1. The type plate 4 mentioned in this embodiment includes 96-well shallow-hole plates, 24-well plates, 48-well plates, and 96-well deep-hole plates, etc. Since the sizes of different specifications of type plates 4 are different, when installing 24-well plates and 48-well plates, the electromagnet disk 1 needs to be replaced or adapted.

[0043] In summary, the bioproduct extractor provided by this invention places a type plate 4 containing magnetic beads and culture solution on an electromagnet disk 1. The electromagnet disk 1 can adjust the magnetic phase, which is reflected in the adsorption and release of magnetic beads. This allows for both enrichment and dispersion of magnetic beads, and matrix-style control, enabling modulation of the magnetic phase for different separation types of magnetic beads, thus accommodating multiple types of plates 4. For bioproducts, this means removing non-specific deposits in different culture solutions, then enriching them onto magnetic beads for further cleaning, until the cleaning step is complete, at which point the bioproducts are released from the magnetic beads, achieving solid-liquid separation. The output of the horizontally adjustable eccentric vibration module is connected to the first moving plate 3. By adjusting the amplitude and vibration frequency bidirectionally, it can accommodate multiple types of plates 4 and shake the type plate 4 above the electromagnet disk 1, ensuring thorough and homogeneous mixing of the culture solution without any external machinery. The temperature control element 2 enables rapid heating and cooling at different times, achieving efficient temperature changes with temporal resolution. Combined with magnetic bead rinsing and temperature increase, this allows for more efficient detachment of bioproducts (nucleic acid-DNA) from the magnetic beads, improving recovery efficiency. In summary, the biological product extractor provided by this invention has the functions of adjusting the magnetic field module for the polymerization and dispersion of magnetic beads, vibration and shaking, and temperature regulation. It avoids the addition and removal of biological products between multiple devices, which not only reduces cross-contamination of biological products, but also improves the preparation efficiency and quality of biological products.

[0044] Furthermore, the horizontally adjustable eccentric vibration module includes a motor 5 and an eccentric shaft 6 arranged vertically. A mounting block 7 is fixedly mounted on the output shaft of the motor 5. The mounting block 7 has a first radially extending slot 71. The upper end of the eccentric shaft 6 is rotatably connected to the first moving plate 3, and the lower end of the eccentric shaft 6 is connected to the first slot 71. The axis of the eccentric shaft 6 is not coaxial with the axis of the output shaft of the motor 5. By radially adjusting the position of the lower end of the eccentric shaft 6 within the first slot 71 and the rotational speed of the motor 5, the amplitude and frequency of the eccentric vibration of the type plate 4 can be adjusted. Two threaded holes 73 on the mounting block 7 can be used for mounting counterweights.

[0045] Furthermore, the side wall of the mounting block 7 is provided with a second strip-shaped hole 72, which communicates with the first strip-shaped hole 71. A fastener 8 passes through the second strip-shaped hole 72 and connects to the lower end of the eccentric shaft 6. The fastener 8 can be, but is not limited to, a pin or a bolt. In this embodiment, a pin passes through the second strip-shaped hole 72 and connects to the portion of the eccentric shaft 6 located within the first strip-shaped hole 71, thus securely assembling the eccentric shaft 6 to the eccentric position of the mounting block 7.

[0046] Furthermore, a bearing pressure plate 9 is installed on the output shaft of the motor 5, which is used to install and limit the position of the bearing 19. The threaded hole 73 on the mounting block 7 can be used to install a counterweight block according to the actual situation, so as to reduce the influence of the mounting block 7 and the eccentric shaft 6 on the rotation of the output shaft of the motor 5, and achieve dynamic balance of the output shaft of the motor 5 in the rotating state.

[0047] Furthermore, the housing of the motor 5 is provided with a plate 10, and the upper surface of the plate 10 is provided with a first linear guide rail 11 extending along the first direction (X direction). The bearing unit also includes a second moving plate 12, and a second slider 18 fixed on the lower end surface of the second moving plate 12 is slidably disposed on the first linear guide rail 11. The upper end surface of the second moving plate 12 is provided with a second linear guide rail 13 extending along the second direction (Y direction), and a first slider 17 fixed on the lower end surface of the first moving plate 3 is slidably disposed on the second linear guide rail 13.

[0048] Specifically, the upper surface of the machine plate 10 is provided with two first linear guide rails 11 arranged parallel to each other and spaced apart, and the upper end surface of the second moving plate 12 is provided with two second linear guide rails 13 arranged parallel to each other and spaced apart. In addition, the second moving plate 12 is provided with a clearance hole 121 for the output shaft of the motor 5 to pass through, so as to avoid structural interference.

[0049] Furthermore, the bioproduct extractor also includes a base plate 14, on which the bottom of the motor 5 is mounted. The base plate 14 has a large surface area, making it easy to place the bioproduct extractor stably on the experimental table. A circuit board module 15 is mounted on the base plate 14, and the circuit board module 15 is electrically connected to the temperature regulating element 2, which can both heat and cool.

[0050] Furthermore, a heat insulation plate 16 is provided between the temperature regulating element 2 and the first moving plate 3 to ensure that the temperature can only heat or cool the type plate 4 on the electromagnet disk 1 upwards, and to prevent the temperature from being transmitted downwards, thereby reducing the temperature influence of the temperature regulating element 2 on the first moving plate 3.

[0051] Note that the above description is merely a preferred embodiment of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and various obvious changes, readjustments, and substitutions can be made without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments, and may include many other equivalent embodiments without departing from the concept of the present invention, the scope of which is determined by the scope of the appended claims.

Claims

1. A biological product extraction instrument, characterized in that, include: The supporting unit includes an electromagnet disk (1), a temperature regulating element (2), and a first moving plate (3) arranged sequentially from top to bottom. The electromagnet disk (1) is used to place a type plate (4) containing magnetic beads. The electromagnet disk (1) is electrically connected to the control module and is used to control the magnetic phase of the electromagnet disk (1). The temperature regulating element (2) is used to regulate the temperature of the type plate (4) above the electromagnet disk (1). A horizontally adjustable eccentric vibration module, the output end of which is connected to the first moving plate (3), is used to shake the type plate (4) above the electromagnet disk (1); The horizontally adjustable eccentric vibration module includes a motor (5) and an eccentric shaft (6) arranged in the vertical direction. The output shaft of the motor (5) is fixedly provided with a mounting block (7). The mounting block (7) has a first strip hole (71) extending radially. The upper end of the eccentric shaft (6) is rotatably connected to the first moving plate (3). The lower end of the eccentric shaft (6) is connected to the first strip hole (71). The axis of the eccentric shaft (6) is not coaxial with the axis of the output shaft of the motor (5). The side wall of the mounting block (7) is provided with a second strip hole (72), which communicates with the first strip hole (71). The fastener (8) passes through the second strip hole (72) and is connected to the lower end of the eccentric shaft (6).

2. The biological product extraction instrument according to claim 1, characterized in that, A bearing pressure plate (9) is installed on the output shaft of the motor (5), and a threaded hole (73) is provided on the mounting block (7) for installing a counterweight.

3. The biological product extraction instrument according to claim 1, characterized in that, The motor (5) has a machine plate (10) on its housing. The upper surface of the machine plate (10) is provided with a first linear guide rail (11) extending in a first direction. The bearing unit also includes a second moving plate (12). The lower end face of the second moving plate (12) is slidably disposed on the first linear guide rail (11). The upper end face of the second moving plate (12) is provided with a second linear guide rail (13) extending in a second direction. The lower end face of the first moving plate (3) is slidably disposed on the second linear guide rail (13).

4. The biological product extraction instrument according to claim 3, characterized in that, The upper surface of the machine plate (10) is provided with two first linear guide rails (11) arranged parallel to each other and spaced apart, and the upper end surface of the second moving plate (12) is provided with two second linear guide rails (13) arranged parallel to each other and spaced apart.

5. The biological product extraction instrument according to claim 3, characterized in that, The second moving plate (12) is provided with a clearance hole (121) for the output shaft of the motor (5) to pass through.

6. The biological product extraction instrument according to claim 1, characterized in that, It also includes a base plate (14), on which the bottom of the motor (5) is mounted. A circuit board module (15) is mounted on the base plate (14), and the circuit board module (15) is electrically connected to the temperature regulating element (2).

7. The biological product extraction instrument according to any one of claims 1-6, characterized in that, A heat insulation plate (16) is provided between the temperature regulating element (2) and the first moving plate (3).

8. The biological product extraction instrument according to any one of claims 1-6, characterized in that, The type plate (4) includes a 96-hole shallow hole plate, a 24-hole plate, a 48-hole plate, and a 96-hole deep hole plate.

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