Quantitative sampling device and drug susceptibility testing instrument

Abstract

This invention discloses a quantitative sampling device, including a quantitative sampling mechanism and a rotation-telescopic motion mechanism for driving the quantitative sampling mechanism to perform sampling. The quantitative sampling mechanism includes a sampling vial holder, on which a sampling vial cap is fixedly mounted. A circular groove is provided on one side of the sampling vial cap, and a small sampling hole communicating with the circular groove is provided on the upper part of the sampling vial cap. A small inlet hole communicating with the circular groove is provided on the lower part of the sampling vial cap. A plunger is provided within the circular groove, and the plunger can rotate relative to the circular groove and move along the axis of the circular groove. The rotation-telescopic motion mechanism includes a base plate, on which a bushing seat is mounted, and a power shaft is mounted within the bushing seat. The base plate also includes a drive assembly and a switching assembly. The drive assembly is used to drive the power shaft to rotate relative to the bushing seat or move axially relative to the bushing seat, and the switching assembly is used to switch between rotational and telescopic motion of the power shaft. This invention also discloses a drug sensitivity analyzer.

Description

Technical Field

[0001] This invention belongs to the field of medical device technology, specifically a quantitative sample addition device and a drug sensitivity sample addition instrument. Background Technology

[0002] Currently, most pipettes use inverted reagent bottles, with a through-hole on the bottle cap. Reagent dispensing is achieved by opening and closing this through-hole. Specifically, existing pipettes require two motors to perform the dispensing action: one motor controls the extension and retraction of the shaft, and the other controls the rotation. The two motors work together to control the rotation and extension / retraction movements, respectively, to complete the dispensing action.

[0003] While the existing method of using two motors to control rotation and extension motion separately can meet the sample loading requirements to some extent, it requires perfect coordination between the two motors in terms of timing. Any deviation in coordination or loss of synchronization between the two motors will lead to inaccurate sample loading results. Furthermore, using two motors increases costs, complicates equipment operation and control, and makes it difficult to control the equipment's size. In addition, with this method of controlling rotation and extension motion separately, the two motors need to alternately start and stop. Frequent start-stop operations over multiple sample loading cycles can adversely affect the motor's lifespan and stability. Summary of the Invention

[0004] In view of this, the purpose of the present invention is to provide a quantitative sample addition device and a drug sensitivity sampler, which realizes the rotation and extension sequence of the shaft through a mechanical structure to achieve quantitative sample addition, which can effectively simplify the control and structure, thereby improving operational reliability, extending service life and reducing volume.

[0005] To achieve the above objectives, the present invention provides the following technical solution:

[0006] This invention first proposes a quantitative sampling device, including a quantitative sampling mechanism and a rotation-telescopic motion mechanism for driving the quantitative sampling mechanism to perform sampling. The quantitative sampling mechanism includes a sampling bottle holder, on which a sampling bottle cap is fixedly mounted. A circular groove is provided on one side of the sampling bottle cap, and a sampling hole communicating with the circular groove is provided on the upper part of the sampling bottle cap. A sample inlet hole communicating with the circular groove is provided on the lower part of the sampling bottle cap. A plunger is provided in the circular groove, and the plunger can rotate relative to the circular groove and move along the axis of the circular groove.

[0007] The rotary-telescopic motion mechanism includes a base plate, on which a bushing seat is mounted, and a power shaft is mounted inside the bushing seat. The base plate is also provided with a drive assembly and a switching assembly. The drive assembly is used to drive the power shaft to rotate relative to the bushing seat or move axially relative to the bushing seat. The switching assembly is used to switch the power shaft between rotary motion and telescopic motion.

[0008] The drive assembly includes a bushing sleeved on the power shaft and a drive mechanism for driving the bushing sleeve to move along the axial direction of the power shaft. The bushing sleeve and the power shaft are provided with a helical groove and a guide pin that cooperate with each other. The helical groove is provided on the bushing sleeve and the guide pin is provided on the power shaft; or, the helical groove is provided on the outer wall of the power shaft and the guide pin is provided inside the bushing sleeve.

[0009] The switching assembly includes a switching seat, at least two switching elements are evenly distributed in a ring on the power shaft, the switching seat has a switching channel for the power shaft to pass through, and the side wall of the switching channel has a clearance groove corresponding to each of the switching elements for the switching elements to pass through; the two ends of the switching seat are respectively provided with limiting seats for limiting the distance the power shaft moves in the axial direction.

[0010] The sample bottle holder is fixedly disposed relative to the base plate, the axis of the circular groove is parallel to or coaxial with the power shaft, and the plunger moves synchronously with the power shaft.

[0011] Furthermore, the inner diameter of the sample dispensing orifice is 0.01-1 mm.

[0012] Furthermore, the inner diameter of the sample dispensing orifice is 0.1-1 mm.

[0013] Furthermore, the inner diameter of the sample dispensing orifice is 0.2-0.8 mm.

[0014] Furthermore, the upper part of the sample bottle cap is provided with a sample dispensing groove, and the sample dispensing hole is located between the bottom of the sample dispensing groove and the circular groove; the lower part of the sample bottle cap is provided with a sample dispensing tip, and the sample inlet hole is located between the sample dispensing tip and the circular groove; the upper part of the sample bottle cap is also provided with a connecting section for connecting a reagent bottle.

[0015] Furthermore, the circular groove has a wide-diameter groove at its opening, forming a limiting step, and the plunger has a large-diameter section that limits and cooperates with the limiting step.

[0016] Furthermore, the plunger is provided with a quantitative groove corresponding to the sample addition hole and the sample injection hole, and the quantitative groove has a connecting hole on its side wall facing the bottom of the circular groove.

[0017] Furthermore, the width of the metering groove along the axial direction of the circular groove is greater than or equal to the distance the plunger moves along the axial direction of the circular groove.

[0018] Furthermore, the base plate is provided with a linear guide rail parallel to the power shaft, and a linear slider is provided on the linear guide rail for sliding cooperation with it. A connecting piece is provided between the linear slider and the bushing for fixed connection.

[0019] Furthermore, there are two spiral grooves, which are arranged in a centrally symmetrical manner with respect to their axis.

[0020] Furthermore, the spiral groove is provided on the bushing, and the power shaft is provided with a pin hole. The axis of the pin hole intersects perpendicularly with the axis of the power shaft. The guide pin is installed in the pin hole, and the two ends of the guide pin are respectively engaged with the two spiral grooves.

[0021] Furthermore, the lengths of the two ends of the spiral groove in its axial direction satisfy the following:

[0022]

[0023] Where L represents the length of both ends of the spiral groove in the axial direction; p represents the lead of the spiral groove.

[0024] Furthermore, a receiving cavity for accommodating the switching element is provided between the switching seat and the two limiting seats, and the width of the receiving cavity in the axial direction of the power shaft is greater than or equal to the thickness of the switching element in the axial direction of the power shaft.

[0025] Furthermore, a spring pressure plate or an end face bearing is provided in the receiving cavity, and an elastic element is provided between the spring pressure plate and the corresponding limiting seat or between the end face bearing and the corresponding limiting seat.

[0026] Furthermore, the drive mechanism includes a drive motor, an eccentric wheel is provided on the output shaft of the drive motor, and a double-hinged connecting rod is provided between the eccentric wheel and the bushing. One end of the double-hinged connecting rod is rotatably engaged with an eccentric shaft provided on the eccentric wheel, and the other end is rotatably engaged with a fixed shaft provided on the bushing. The output shaft, eccentric shaft and fixed shaft of the drive motor are all perpendicular to the power shaft.

[0027] The present invention also proposes a drug sensitivity tester, including a drug sensitivity tester holder for mounting a drug sensitivity tester and a quantitative sample dispensing device for dispensing a sample onto the drug sensitivity tester, wherein the quantitative sample dispensing device adopts the quantitative sample dispensing device described above.

[0028] The beneficial effects of this invention are as follows:

[0029] The sample dispensing process of the quantitative dispensing device of the present invention is as follows: When the quantitative dispensing device is in the first state, the plunger is located in the first position close to the bottom of the circular groove. At this time, the switching element is located between the switching seat and the limiting seat facing the quantitative dispensing mechanism. When the drive mechanism drives the bushing to move in the first direction along the axial direction of the power shaft, the axial limiting fit between the spiral groove and the guide pin is used to make the power shaft move linearly relative to the bushing seat in the axial direction. During this process, the plunger moves towards the opening of the circular groove, forming a negative pressure space between the plunger and the bottom of the circular groove. Under the action of the driving mechanism, the sample is driven to enter the plunger through the sample inlet orifice; when the switching element passes through the switching channel and is axially limited by the limiting seat on the side opposite to the quantitative sample dispensing mechanism, the plunger is in the second position away from the bottom of the circular groove, and the quantitative sample dispensing device is in the second state. At this time, the power shaft can no longer move axially in the first direction; the driving mechanism continues to drive the bushing to move in the first direction. Under the action of the spiral groove and the guide pin, the power shaft rotates relative to the bushing seat, so that the sample in the plunger is aligned with the sample inlet orifice, and the quantitative sample dispensing device is in the third state; the driving mechanism drives the bushing to move in the first direction. The power shaft moves axially in a second direction opposite to the first direction. Utilizing the axial limiting fit between the spiral groove and the guide pin, the power shaft first moves linearly relative to the bushing seat axially. During this process, the plunger moves towards the bottom of the circular groove. Within the air plunger between the plunger and the bottom of the circular groove, under the action of air pressure, the sample is driven to flow out of the plunger through the injection orifice to achieve sample injection. When the switching element passes through the switching channel and engages with the limiting seat near the quantitative sample dispensing mechanism on the axial limiting side, the plunger is located in the first position near the bottom of the circular groove, at the quantitative sample dispensing device. In the fourth state, the power shaft can no longer move axially in the second direction. The drive mechanism continues to drive the bushing to move in the second direction. Under the action of the spiral groove and the guide pin, the power shaft rotates relative to the bushing seat, and the quantitative sampling device returns to the first state. In summary, the quantitative sampling device and drug sensitivity sampler of the present invention only need to drive the bushing to reciprocate along the axial direction of the power shaft to achieve the rotation and extension sequence of the shaft through a mechanical structure to achieve quantitative sampling. This can effectively simplify the control and structure, thereby improving operational reliability, extending service life and reducing volume. Attached Figure Description

[0030] To make the objectives, technical solutions, and beneficial effects of this invention clearer, the following figures are provided for illustration:

[0031] Figure 1 A schematic diagram of the structure of the drug sensitivity continuous sampling system using the quantitative sampling device of this invention;

[0032] Figure 2 for Figure 1 A three-dimensional image;

[0033] Figure 3This is a schematic diagram of the structure of an embodiment of the quantitative sampling device of the present invention;

[0034] Figure 4 for Figure 3 The AA cross-sectional view is specifically a diagram of the quantitative sample dispensing device in its first state.

[0035] Figure 5 for Figure 4 Enlarged view of region C;

[0036] Figure 6 for Figure 3 BB cross-sectional view;

[0037] Figure 7 for Figure 6 Enlarged view of region D;

[0038] Figure 8 This is a perspective view of the quantitative sample addition device in this embodiment;

[0039] Figure 9 A diagram showing the state of the quantitative sample dispensing device in its second state;

[0040] Figure 10 The diagram shows the state of the quantitative sample dispensing device in its third state.

[0041] Figure 11 The diagram shows the state of the quantitative sample dispensing device in its fourth state.

[0042] Explanation of reference numerals in the attached figures:

[0043] 100-Drug susceptibility plate holder assembly; 101-Drug susceptibility plate; 102-Sample dispensing port; 103-Fixing plate; 104-Drug susceptibility plate holder; 105-First track; 106-First slider; 107-First synchronous pulley; 108-First drive motor; 109-Second track; 110-Second slider; 111-Second synchronous pulley; 112-Second drive motor;

[0044] 200-Quantitative sampling device; 210-Quantitative sampling mechanism; 211-Sampling bottle holder; 212-Sampling bottle cap; 213-Plunger groove; 214-Sampling orifice; 215-Inlet orifice; 216-Reagent bottle; 217-Sampling groove; 218-Connecting section; 219-Sampling nozzle; 220-Plunger; 221-Quantitative groove; 222-Connecting hole; 223-Mounting base;

[0045] 230 - Rotary-telescopic motion mechanism; 231 - Base plate; 232 - Bushing seat; 233 - Power shaft; 233a - Switching component; 234 - Bushing; 235 - Spiral groove; 236 - Guide pin; 237 - Linear guide rail; 238 - Linear slider; 239 - Connecting component; 240 - Drive motor; 241 - Eccentric wheel; 242 - Double hinge connecting rod; 243 - Eccentric shaft; 244 - Fixed shaft; 250 - Switching seat; 251 - Switching channel; 252 - Relief groove; 253 - Limiting seat; 254 - Receiving cavity; 255 - Spring pressure plate; 256 - Elastic element. Detailed Implementation

[0046] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, so that those skilled in the art can better understand and implement the present invention. However, the embodiments described are not intended to limit the present invention.

[0047] like Figure 1-2As shown, the continuous drug sensitivity testing system of this embodiment includes a drug sensitivity plate holder assembly 100 and a quantitative sampling device 200 for adding samples to a drug sensitivity plate 101. The drug sensitivity plate 101 has a plurality of sampling holes 102 arrayed on it. Specifically, the drug sensitivity plate holder assembly 100 of this embodiment includes a fixing plate 103 and a drug sensitivity plate holder 104 for mounting the drug sensitivity plate 101. The fixing plate 103 is provided with a planar movement mechanism for driving the drug sensitivity plate holder 104 to move in a plane. The planar movement mechanism can be implemented in various existing ways. In this embodiment, the planar movement mechanism includes a first track 105 mounted on the fixing plate 103 and a first slider 106 slidably engaged with the first track 105. The fixing plate 103 of this embodiment is provided with a first driving mechanism for driving the first slider 106 to move along the first track 105. Specifically, the first driving mechanism in this embodiment includes two first synchronous pulleys 107 respectively disposed at both ends of the first track 105, a first synchronous belt (not shown in the figure) between the two first synchronous pulleys 107, and a first connecting block (not shown in the figure) fixedly connecting the first synchronous belt and the first slider 106. A first driving motor 108 is provided on the fixing plate 103 and is pulsatorically connected to one of the first synchronous pulleys 107. Thus, the first driving motor 108 drives the first synchronous pulleys 107 to move the first synchronous belt, thereby driving the first slider 106 to slide along the first track 105. In this embodiment, a second track 109 perpendicular to the first track 105 and a second slider 110 slidably engaged with the second track 109 are mounted on the first slider 106, and the drug sensitivity plate holder 104 is mounted on the second slider 110. In this embodiment, a second driving mechanism is provided on the first slider 106 for driving the second slider 110 to move along the second track 109. The second driving mechanism in this embodiment includes two second synchronous pulleys 111 respectively disposed at both ends of the second track 109. A second synchronous belt (not shown in the figure) is provided between the two second synchronous pulleys 111. A second connecting block (not shown in the figure) is fixedly connected between the second synchronous belt and the second slider 110. A second driving motor 112 is provided on the first slider 106 and is drivenly connected to one of the second synchronous pulleys 111. Thus, the second driving motor 112 drives the second synchronous pulley 111 to move the second synchronous belt, thereby driving the second slider 110 to slide along the second track 109. Since the first track 105 and the second track 109 are perpendicular to each other, the planar movement mechanism can drive the drug sensitivity plate holder 104 to move along the mutually perpendicular first track 105 and second track 109, that is, drive the drug sensitivity plate holder 104 to move in a plane parallel to the first track 105 and the second track 109.

[0048] like Figure 3-7As shown, the quantitative sampling device 200 of this embodiment includes a quantitative sampling mechanism 210 and a rotation-extension motion mechanism 230 for driving the quantitative sampling mechanism to perform sample dispensing. The quantitative sampling mechanism 210 of this embodiment includes a sample bottle holder 211, on which a sample bottle cap 212 is fixedly mounted. A plunger groove 213 is provided on one side of the sample bottle cap 212, a sample dispensing hole 214 communicating with the plunger groove 213 is provided at the upper part of the sample bottle cap 212, and a sample inlet hole 215 communicating with the plunger groove 213 is provided at the lower part of the sample bottle cap 212. Specifically, the diameter of the sample dispensing hole 214 should be set small enough so that the sample will not flow out from the sample dispensing hole 214 under the action of the surface tension of the liquid, but air can pass through to achieve the purpose of balancing the air pressure inside and outside the reagent bottle 216. Specifically, in this embodiment, the inner diameter of the sample dispensing orifice is 0.01-1 mm; preferably, the inner diameter of the sample dispensing orifice can be 0.1-1 mm; more preferably, the inner diameter of the sample dispensing orifice is 0.2-0.8 mm. The upper part of the sample dispensing bottle cap 212 is provided with a sample dispensing groove 217, and the sample dispensing orifice 214 is disposed between the bottom of the sample dispensing groove 217 and the plunger groove 213. The upper part of the sample dispensing bottle cap 212 is also provided with a connecting section 218 for connecting the reagent bottle 216. The inner wall of the connecting section 218 is provided with internal threads, and the bottle mouth of the reagent bottle 216 is provided with external threads that mate with the connecting section 218. In this embodiment, the lower part of the sample dispensing bottle cap 212 is provided with a sample dispensing tip 219, and the sample inlet orifice 215 is disposed between the sample dispensing tip 219 and the plunger groove 213. In this embodiment, the sample dispensing orifice 214 and the sample inlet orifice 215 are coaxially arranged.

[0049] A plunger 220 is provided inside the plunger groove 213. The plunger 220 can rotate relative to the plunger groove 213 and move along the axis of the plunger groove 213. In this embodiment, the plunger 220 is provided with a quantitative groove 221 corresponding to the sample dispensing orifice 214 and the sample injection orifice 215. A connecting hole 222 is provided on the side wall of the quantitative groove 221 facing the bottom of the plunger groove 213. The quantitative groove 221 is used to receive the sample from the sample dispensing orifice 214 and inject the sample through the sample injection orifice 215. The connecting hole 222 is used to connect the quantitative groove 221 with the plunger groove 213 and to keep the air pressure in the quantitative groove 221 consistent with the air pressure in the plunger groove 213. In a preferred embodiment of this example, the width of the quantitative groove 221 along the axial direction of the plunger groove 213 is greater than or equal to the distance the plunger 220 moves along the axial direction of the plunger groove 213. In this way, during the reciprocating movement of the plunger 220 along the axial direction of the plunger groove 213, the sample inlet 214 or sample dispensing orifice 215 is always connected to the quantitative groove 221.

[0050] like Figure 4 , 6As shown in Figure 8, the rotary-telescopic motion mechanism 230 of this embodiment includes a base plate 231. A mounting seat 223 is provided on the fixing plate 103 of this embodiment, and the base plate 231 is mounted on the mounting seat 223. A bushing seat 232 is mounted on the base plate 231, and a power shaft 233 is installed inside the bushing seat 232. The axis of the power shaft 233 is coaxial or parallel to the axis of the plunger groove 213, and the plunger 220 moves synchronously with the power shaft 233. In this embodiment, the plunger 220 and the power shaft 233 are fixedly connected. The base plate 231 of this embodiment also includes a drive assembly and a switching assembly. Specifically, the drive assembly is used to drive the power shaft 233 to rotate relative to the bushing seat 232 or move axially relative to the bushing seat 232, and the switching assembly is used to switch between rotary and telescopic motion of the power shaft 233.

[0051] like Figure 8 As shown, in this embodiment, the drive assembly includes a bushing 234 sleeved on the power shaft 233 and a drive mechanism for driving the bushing 234 to move axially along the power shaft 233. A helical groove 235 and a guide pin 236 are provided between the bushing 234 and the power shaft 233 for mutual engagement. Specifically, when the helical groove 235 is provided on the bushing 234, the guide pin 236 is provided on the power shaft 233; when the helical groove 235 is provided on the outer wall of the power shaft 233, the guide pin 236 is provided inside the bushing 234. Utilizing the limiting engagement relationship between the guide pin 236 and the helical groove 235 in the axial direction, when the resistance experienced by the power shaft 233 in the axial direction decreases, the bushing 234 can drive the power shaft 233 to move axially; when the resistance experienced by the power shaft 233 in the axial direction is large, the bushing 234 can drive the power shaft 233 to rotate. Specifically, at least two spiral grooves 235 are evenly distributed in a ring, and the spiral grooves 235 are disposed on the bushing 234. A pin hole is provided on the power shaft 233, the axis of which intersects perpendicularly with the axis of the power shaft 233. A guide pin 236 is installed in the pin hole and extends into the spiral groove 235. In this embodiment, two spiral grooves 235 are evenly distributed in a ring, and the pin holes on the power shaft 233 corresponding to the two spiral grooves 235 are coaxial and interconnected. A guide pin 236 is installed in the interconnected small hole, and both ends of the guide pin 236 extend into the two spiral grooves 235 respectively. The lengths of the two ends of the spiral groove 235 in its axial direction satisfy the following:

[0052] L=p*α / (2π)

[0053] Where L represents the axial length of both ends of the helical groove 235; p represents the lead of the helical groove 235; and α is the desired rotation angle of the mechanism. In this embodiment, the rotation angle is designed to be π, that is:

[0054]

[0055] To further constrain the movement of the bushing 234 along the axial direction and prevent it from rotating relative to the drive shaft 233, such as Figure 8 As shown, in this embodiment, a linear guide rail 237 parallel to the power shaft 233 is provided on the base plate 231. A linear slider 238 is provided on the linear guide rail 237 and slides therewith. A connecting piece 239 is provided between the linear slider 238 and the bushing 234 for fixed connection. In this way, under the guidance of the linear guide rail 237, the accuracy of the bushing 234 moving in a straight line can be further improved and its rotation relative to the bushing seat 232 can be prevented. Specifically, there are many ways to drive the bushing 234 to make linear motion in the prior art, such as threaded screw mechanism, linear motor, etc. In this embodiment, the driving mechanism includes a drive motor 240. An eccentric wheel 241 is provided on the output shaft of the drive motor 240. A double hinge connecting rod 242 is provided between the eccentric wheel 241 and the bushing 234. One end of the double hinge connecting rod 242 is rotatably engaged with the eccentric shaft 243 provided on the eccentric wheel 241, and the other end is rotatably engaged with the fixed shaft 244 provided on the bushing 234. The output shaft, eccentric shaft 243, and fixed shaft 244 of the drive motor 240 are all perpendicular to the power shaft 233. Thus, the drive motor 240 drives the eccentric wheel 241 to rotate, the eccentric wheel 241 drives the double hinge connecting rod 242 to swing and drives the bushing 234 to reciprocate along the axial direction of the power shaft 233. Furthermore, the distance that the power shaft 233 reciprocates along its axial direction can be precisely controlled by the eccentricity between the eccentric shaft 243 and the eccentric wheel 241.

[0056] like Figure 6-7As shown, the switching assembly in this embodiment includes a switching seat 250. At least two switching elements 233a are evenly distributed in a ring on the power shaft 233. The switching seat 250 has a switching channel 251 for the power shaft 233 to pass through. The side wall of the switching channel 251 has a relief groove 252 corresponding to the switching elements 233a for the switching elements to pass through. Both ends of the switching seat 250 are respectively provided with limiting seats 253 for limiting the distance the power shaft 233 moves in the axial direction. Since the plunger 220 needs to rotate 180° each time, the number of switching elements 233a is set to an even number. In this way, after the plunger 220 rotates 180°, each switching element 233a can still be aligned with a relief groove 252, so that the switching element 233a can pass through the switching channel 251. Specifically, in this embodiment, there are two switching elements 233a and two clearance grooves 252. Before and after the plunger 220 rotates, the two switching elements 233a are aligned with the two clearance grooves 252 respectively. Specifically, there are receiving cavities 254 between the switching seat 250 and the two limiting seats 253 for accommodating the switching elements 233a. The width of the receiving cavity 254 in the axial direction of the power shaft 233 is greater than or equal to the thickness of the switching element 233a in the axial direction of the power shaft 233. Thus, when the switching element 233a is in limiting engagement with the limiting seat 253, the switching element 233a and the switching channel 251 are misaligned. When the power shaft 233 rotates, the switching element 233a will not interfere with the switching channel 251. In this embodiment, a spring pressure plate 255 is provided in the receiving cavity 254, and an elastic element 256 is provided between the spring pressure plate 255 and the corresponding limiting seat 253. In some other embodiments, an end-face bearing may be provided within the receiving cavity 254, and an elastic element 256 may be provided between the end-face bearing and the corresponding limiting seat 253. By providing a spring pressure plate 255 or an end-face bearing, friction between the switching member 233a and the elastic element 256 can be prevented during the rotation of the power shaft 233, and the end-face bearing can also reduce the resistance to the rotation of the power shaft 233. In this embodiment, the elastic element 256 is a spring.

[0057] Specifically, this embodiment also proposes a drug sensitivity testing instrument including the quantitative sampling device 200 as described above.

[0058] The following describes the specific implementation method of the continuous drug susceptibility testing method in conjunction with the aforementioned continuous drug susceptibility testing system.

[0059] The continuous drug susceptibility testing method of this embodiment includes the following steps:

[0060] Step 1: Install the drug sensitivity plate 101 on the drug sensitivity plate holder 104; put the quantitative sample addition device 200 in the first state, such as... Figure 4As shown, at this time, the plunger 220 is located in the first position near the bottom of the plunger groove 213, and the switching element 233a is located between the switching seat 250 and the limiting seat 253 facing the quantitative sample dispensing mechanism 210.

[0061] Step 2: Start the drive motor 240, and use the drive mechanism to drive the bushing 234 to move along the axial direction of the power shaft 233 in the first direction. Utilizing the axial limiting fit between the spiral groove 235 and the guide pin 236, the resistance to the axial movement of the power shaft 233 is small. The power shaft 233 first moves linearly along the axial direction relative to the bushing seat 232. During this process, the plunger 220 moves towards the groove opening of the plunger groove 213, forming a negative pressure between the plunger 220 and the bottom of the plunger groove 213. Under the action of the negative pressure, the sample enters the quantitative groove 221 of the plunger 220 through the sample dispensing orifice 214. When the switching element 233a passes through the switching channel 251 and is axially limited by the limiting seat 253 on the side opposite to the quantitative dispensing mechanism 210, and the power shaft 233 can no longer move along the axial direction in the first direction, the plunger 220 is in the second position away from the bottom of the plunger groove 213, and the quantitative dispensing device 200 is in the second state. Figure 9 As shown; due to the axial limiting effect of the limiting seat 253 on the switching component 233a, the resistance to axial movement of the power shaft 233 increases, and the resistance to rotation of the power shaft 233 is less than the resistance to axial movement. At this time, the drive mechanism continues to drive the bushing 234 to move along the first direction. Under the action of the spiral groove 235 and the guide pin 236, the power shaft 233 rotates relative to the bushing seat 232, so that the sample in the plunger 220 is aligned with the injection hole 215, and the quantitative sample addition device 200 is in the third state, such as Figure 10 As shown;

[0062] Before the quantitative sampling device 200 is in the third state, the planar moving mechanism drives the sampling hole 102 of the drug sensitivity plate 101, which needs to be sampled, to be located directly below the sample inlet hole 215.

[0063] Step 3: Using the drive mechanism, drive bushing 234 moves along the axial direction of power shaft 233 in a second direction opposite to the first direction. At this time, the resistance to the axial movement of power shaft 233 is small. Utilizing the axial limiting fit between spiral groove 235 and guide pin 236, power shaft 233 first moves linearly along the axial direction relative to bushing seat 232. During this process, plunger 220 moves towards the bottom of plunger groove 213. Air between plunger 220 and the bottom of plunger groove 213 enters plunger 220. Under the action of air pressure, the sample is driven to flow out through injection hole 215 and injected into the sample dispensing hole 102 located directly below it. When switching element 233a passes through switching channel 251 and is axially limited by limiting seat 253 near the quantitative dispensing mechanism 210, and power shaft 233 can no longer move along the axial direction in the second direction, plunger 220 is located in the first position near the bottom of plunger groove 213, and quantitative dispensing device 200 is in the fourth state. Figure 11 As shown;

[0064] Step 4: Continue to drive the bushing to move along the second direction using the drive mechanism. Since the switching part 233a is in a limiting engagement with the limiting seat 253 on the side near the quantitative sample dispensing mechanism 210, the resistance to the axial movement of the power shaft 233 is much greater than the resistance to the rotation of the power shaft 233. Thus, under the action of the spiral groove 235 and the guide pin 236, the power shaft 233 rotates relative to the bushing seat 232 until the quantitative sample dispensing device 200 returns to the first state, and Step 2 is executed to achieve the technical objective of continuously dispensing samples to different sample dispensing positions 102.

[0065] That is, during each sample addition process, the time interval between the return of the quantitative sample addition device 200 from the fourth state to the first state and from the first state to the third state can be used as the time for the planar moving mechanism to drive the drug sensitivity plate holder 104 to move so that the set sample addition hole position 102 is aligned with the sample addition hole 215. The process from the third state to the fourth state of the quantitative sample addition device 200 is the sample addition process, during which the drug sensitivity plate holder 104 remains in a fixed position.

[0066] The above-described embodiments are merely preferred embodiments provided to fully illustrate the present invention, and the scope of protection of the present invention is not limited thereto. Equivalent substitutions or modifications made by those skilled in the art based on the present invention are all within the scope of protection of the present invention. The scope of protection of the present invention is defined by the claims.

Claims

1. A quantitative sample adding device comprising a quantitative sample adding mechanism and a rotary- telescopic motion mechanism for driving the quantitative sample adding mechanism to add a sample, characterized in that: The quantitative sampling mechanism includes a sampling bottle holder, on which a sampling bottle cap is fixedly mounted. A circular groove is provided on one side of the sampling bottle cap. A sampling hole communicating with the circular groove is provided on the upper part of the sampling bottle cap, and an injection hole communicating with the circular groove is provided on the lower part of the sampling bottle cap. A plunger is provided within the circular groove, and the plunger can rotate relative to the circular groove and move along the axis of the circular groove. A quantitative groove is provided on the plunger corresponding to the sampling hole and the injection hole, and a connecting hole is provided on the side wall of the quantitative groove facing the bottom of the circular groove. The rotary-telescopic motion mechanism includes a base plate, on which a bushing seat is mounted, and a power shaft is mounted inside the bushing seat. The base plate is also provided with a drive assembly and a switching assembly. The drive assembly is used to drive the power shaft to rotate relative to the bushing seat or move axially relative to the bushing seat. The switching assembly is used to switch the power shaft between rotary motion and telescopic motion. The drive assembly includes a bushing sleeved on the power shaft and a drive mechanism for driving the bushing sleeve to move along the axial direction of the power shaft. The bushing sleeve and the power shaft are provided with a helical groove and a guide pin that cooperate with each other. The helical groove is provided on the bushing sleeve and the guide pin is provided on the power shaft; or, the helical groove is provided on the outer wall of the power shaft and the guide pin is provided inside the bushing sleeve. The switching assembly includes a switching seat, at least one switching element is evenly distributed in a ring on the power shaft, the switching seat has a switching channel for the power shaft to pass through, and the side wall of the switching channel has a clearance groove corresponding to the switching element for the switching element to pass through; the two ends of the switching seat are respectively provided with limiting seats for limiting the distance the power shaft moves in the axial direction. The sample bottle holder is fixedly disposed relative to the base plate, the axis of the circular groove is parallel to or coaxial with the power shaft, and the plunger moves synchronously with the power shaft.

2. The dosing device of claim 1, wherein: The inner diameter of the sample dispensing orifice is 0.01-1 mm.

3. The dosing device of claim 2, wherein: The inner diameter of the sample dispensing orifice is 0.1-1 mm.

4. The dosing device of claim 3, wherein: The inner diameter of the sample dispensing orifice is 0.2-0.8 mm.

5. The quantitative sample adding device according to claim 1, characterized in that: The upper part of the sample bottle cap is provided with a sample dispensing groove, and the sample dispensing hole is located between the bottom of the sample dispensing groove and the circular groove; the lower part of the sample bottle cap is provided with a sample dispensing tip, and the sample inlet hole is located between the sample dispensing tip and the circular groove; the upper part of the sample bottle cap is also provided with a connecting section for connecting a reagent bottle.

6. The quantitative sample adding device according to claim 1, characterized in that: The circular groove has a wide-diameter groove at its opening, forming a limiting step, and the plunger has a large-diameter section that limits and matches the limiting step.

7. The quantitative sample adding device according to claim 1, characterized by: The width of the metering groove along the axial direction of the circular groove is greater than or equal to the distance the plunger moves along the axial direction of the circular groove.

8. The quantitative sample adding device according to claim 1, characterized in that: The base plate is provided with a linear guide rail parallel to the power shaft, and a linear slider is provided on the linear guide rail for sliding cooperation with it. A connecting piece is provided between the linear slider and the bushing for fixed connection.

9. The quantitative sample adding device according to claim 1, characterized in that: The spiral groove is provided in two parts, and the two spiral grooves are arranged in a centrally symmetrical manner with respect to their axis.

10. The dosing device of claim 9, wherein: The spiral groove is provided on the bushing, and the power shaft is provided with a pin hole. The axis of the pin hole intersects perpendicularly with the axis of the power shaft. The guide pin is installed in the pin hole, and the two ends of the guide pin are respectively engaged with the two spiral grooves.

11. The quantitative sample adding device according to claim 1, characterized in that: The lengths of the two ends of the spiral groove in its axial direction satisfy the following: wherein denotes the length of the helical groove in axial direction at both ends; denotes the lead of the helical groove.

12. The quantitative sample adding device according to claim 1, characterized in that: The switching seat and the two limiting seats are respectively provided with a receiving cavity for accommodating the switching component, and the width of the receiving cavity in the axial direction of the power shaft is greater than or equal to the thickness of the switching component in the axial direction of the power shaft.

13. The dosing device of claim 12, wherein: The cavity is provided with a spring pressure plate or an end face bearing, and an elastic element is provided between the spring pressure plate and the corresponding limiting seat or between the end face bearing and the corresponding limiting seat.

14. The quantitative sample adding device according to claim 1, characterized in that: The drive mechanism includes a drive motor, an eccentric wheel on the output shaft of the drive motor, a double-hinged connecting rod between the eccentric wheel and the bushing, one end of the double-hinged connecting rod being rotatably engaged with an eccentric shaft on the eccentric wheel, and the other end being rotatably engaged with a fixed shaft on the bushing; the output shaft, eccentric shaft and fixed shaft of the drive motor are all perpendicular to the power shaft.

15. A drug susceptibility pippette comprising a drug susceptibility plate rack for mounting a drug susceptibility plate and a quantitative pippette device for pippetting to the drug susceptibility plate, characterized in that: The quantitative sampling device is the quantitative sampling device as described in any one of claims 1-14.

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