A composition derived from an extreme environment for repairing the skin barrier

Abstract

The method provides a skin barrier repairing composition derived from an extreme environment, combines Antarctic chlorella extract, ectoine and glycerol glucoside, the composition has a significant synergistic effect in repairing the skin barrier, and the cosmetic applying the composition has excellent repairing effect, fundamentally solves a series of problems of the skin, and has a good application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular to a composition for repairing skin barrier derived from extreme environment. BACKGROUND

[0002] With the increase of age, the acceleration of life rhythm, and the increasing pressure of life and work, the metabolism of skin begins to slow down, the content of collagen, elastin, mucopolysaccharide and other substances in the skin decreases to varying degrees, and the skin barrier is damaged. When the skin barrier is damaged, the epidermis of the skin becomes thin, and phenomena such as decrease of skin water content and increase of transdermal water loss rate occur. However, the weakening of the skin barrier also makes the skin more susceptible to the influence and harm of the external environment, and ultraviolet rays and environmental pollution can cause the generation of skin inflammation, thereby further deteriorating the skin barrier.

[0003] In nature, there are often some extreme environmental conditions that are not conducive to the survival of plants, animals and microorganisms, such as high temperature, severe cold, drought, extreme pH, high pressure, high salt and high radiation intensity, etc. They limit the growth and reproduction of most organisms. However, there is still a large class of organisms that can survive in such extreme environments.

[0004] Antarctic chondrus crispus extract is derived from Antarctic ice sea brown algae (kelp). Antarctic ice sea brown algae is the largest brown algae in the world, with leaves up to 15 meters long, thick and soft, and tough texture, which can well adapt to the extreme environment of cold, storm, high pressure and strong ultraviolet in the Antarctic. This strong vitality is due to the presence of two key substances in its body: glycine betaine and polyphenol. Glycine betaine can protect proteins, enzymes and membranes in extreme environments, maintain tissue integrity and tightness, prevent water loss, and quickly restore the water balance of keratinocytes. Polyphenol can effectively reduce the strong oxidation caused by ultraviolet irradiation and rapid temperature stress changes, and protect DNA from damage.

[0005] Glycerol glucoside is composed of glucose and glycerol. It is the main active ingredient of Myrothamnus flabellifolia (resurrection plant), which is essential for the survival of the resurrection plant in long-term drought environment. It enables the resurrection plant to survive in harsh environments (drought, extreme temperature, high salinity), protecting the resurrection plant from the harsh conditions of its habitat. Studies have shown that glycerol glucoside can penetrate into the dermis, has long-lasting moisturizing, repairing and soothing effects, and its multi-hydroxyl structure can maintain cell water and promote the synthesis of aquaporin at the cellular level to fully hydrate cells, thus having good moisturizing effect. Glycerol glucoside can also promote the expression of type I collagen, thereby enhancing skin elasticity and collagen, and promoting skin barrier repair. In addition, glycerol glucoside can significantly reduce the release of inflammatory factors such as IL-1β and IL-1α, and significantly improve cell healing rate, thus having good anti-inflammatory and soothing effects.

[0006] Ectoin was first isolated from a special bacterium, Halophilic bacteria, in the salt and alkaline land of Wadi Natrun in Egypt in 1985. Halophilic bacteria has survived for hundreds of millions of years on earth. It is a bacterium that lives in high-salinity environments such as desert salt and alkaline land, volcanic crater, salt lake and deep sea. Halophilic bacteria can survive for hundreds of millions of years under the protection of Ectoin in the body. It is because Ectoin can balance the osmotic pressure inside and outside the bacterial cell, protect the cell membrane, and maintain the balance and stability of the genetic material of the life metabolism nucleic acid of the bacterium. In extremely dry, high and low temperature, strong ultraviolet radiation and high salt environment, halophilic bacteria can maintain normal life state. Studies have shown that Ectoin has good repair and protection effect on skin. It has the following biological functions: osmotic pressure gene regulator, anti-stress protective effect, molecular chaperone effect, radiation protection and moisturizing effect. SUMMARY

[0007] At present, it is very rare to combine Antarctic chlorella extract, glycosylglycerol and Ectoin which are derived from extreme environments and study their effects and specific targets in repairing skin barrier.

[0008] In the prior art, there is little data on the application of Antarctic chlorella extract in patents. Usually, it is added to the formula as a moisturizing and soothing ingredient. The efficacy research is based on the whole formula, and does not involve the skin care mechanism target research of Antarctic chlorella extract single component. In the existing patents, glycosylglycerol and Ectoin are often used as moisturizing, soothing and repairing raw materials in the formula, and the efficacy mechanism research of single component is much. However, the skin care efficacy mechanism research of the combination of Antarctic chlorella, glycosylglycerol and Ectoin is very rare.

[0009] The technical problem to be solved by the present application is that the existing patents do not study the mechanism target of Antarctic chlorella and Antarctic chlorella extract, Ectoin and glycosylglycerol composition in repairing skin barrier.

[0010] In order to solve the above problems, the present application provides a repairing composition, which comprises Antarctic chlorella extract, Ectoin and glycosylglycerol.

[0011] Further, the concentration of Antarctic chlorella extract in the composition is 0.00001%-5%, the concentration of Ectoin is 0.00001%-2%, and the concentration of glycosylglycerol is 0.00001%-3%.

[0012] This invention provides the use of a composition for preparing a repairing skin care formulation, the composition comprising Antarctic algae extract, ectoine, and glyceryl glucoside.

[0013] Furthermore, the composition achieves its purpose of repairing the skin barrier by promoting barrier protein expression and reducing transepidermal water loss, or by any one or both of these.

[0014] Furthermore, the barrier protein is filaggrin (FLG).

[0015] Filagrin (FLG) is a basic protein component of keratinocytes (transparent granules) in the basal cells of mammalian skin. It can specifically bind to intermediate filaments. Basal cells are undifferentiated juvenile cells (stem cells) with active proliferative capacity. As newly formed cells migrate to the superficial layers, they gradually differentiate to form the cells of the remaining layers of the epidermis; therefore, the basal layer is also called the germinal layer. Electron microscopy reveals numerous rough endoplasmic reticulum and tonofilaments within the cytoplasm.

[0016] This invention demonstrates, in a 3D skin damage model stimulated by the anionic surfactant sodium dodecyl sulfate (SLS), that extracts of Antarctic algae, ectoine, glyceryl glucoside, and their composition can repair the skin barrier by promoting the expression of filaggrin (FLG) and reducing the transepidermal water loss (TEWL) value.

[0017] Significant advantages of this invention:

[0018] 1. The method of this invention clarifies the synergistic effect mechanism and target of the combination of Antarctic algae extract, ectoine and glyceryl glucoside in repairing the skin barrier;

[0019] 2. The method of the present invention provides a composition comprising Antarctic algae extract, ectoine and glyceryl glucoside. This composition can be applied to a variety of cosmetics, and cosmetics using this composition have excellent skin barrier repair effects. Attached image description:

[0020] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Appendix Figure 1 Results of tests on the effects of single raw materials and compositions on filaggrin FLG

[0022] Appendix Figure 2Test results of the effects of single raw materials and compositions on trans-epidermal water loss rate (TEWL)

[0023] Embodiment

[0024] The Antarctica Chlorella extract in the following examples was purchased from GREENTECH SA, with the trade name ANTARCTICA ALGAE HYDROGLYCOLIC EXTRACT (BG) (300034); the ectoine was purchased from Huaxi Biotechnology Co., Ltd., with the trade name Xi'an Yan TM Ectoine; glycerol glucoside was purchased from BASF (China) Co., Ltd., with the trade name Aquaporin.

[0025] Embodiment 1: A composition for repairing skin barrier derived from an extreme environment

[0026] The method of the present application provides a composition for repairing skin barrier derived from an extreme environment, mixing 0.1% of Antarctica Chlorella extract, 0.1% of ectoine and 0.5% of glycerol glucoside to obtain the composition of the method of the present application, then stimulating a 3D epidermal skin model with an anionic surfactant sodium dodecyl sulfate (SLS) to construct an in vitro skin damage model, detecting the changes of filaggrin FLG and trans-epidermal water loss rate (TEWL) value and recording.

[0027] Embodiment 2: Effects of single raw materials and compositions on filaggrin FLG

[0028] The invention method described in embodiment 1 was used to combine 0.1% of Antarctica Chlorella extract, 0.1% of ectoine and 0.5% of glycerol glucoside into different sample groups for experiments, and the experimental results are shown in Table 1 and Figure 1.

[0029] Table 1: Effects of single raw materials and compositions on filaggrin FLG

[0030]

[0031]

[0032] From the experimental data in Table 1, the combination of 0.1% Antarctica Chlorella extract + 0.1% ectoine + 0.5% glycerol glucoside has a more significant effect on promoting the generation of filaggrin FLG, which is significantly better than the single raw material group and the two raw material component groups, and has obvious inter-group differences, so the composition can produce a significant effect of repairing skin barrier through the synergistic mechanism of compounding. The three compositions have obvious synergistic effect, so the promotion effect is obviously better than using them alone and using two of them.

[0033] The combination of 0.00001%-5% of the Antarctic haircap moss extract, 0.00001%-2% of the ectoine and 0.00001-3% of the glycerol glucoside is found to have a significantly better promoting effect than the single use. After variance analysis, the combination of 0.1% of the Antarctic haircap moss extract, 0.1% of the ectoine and 0.5% of the glycerol glucoside has a significant difference (P less than 0.05) with other sample groups, and the specific analysis process and data are omitted.

[0034] Case three: the influence of single material and combination on the TEWL value

[0035] The 0.1% of the Antarctic haircap moss extract, the 0.1% of the ectoine and the 0.5% of the glycerol glucoside are combined according to the inventive method in case one to form different sample groups for experiments, and the experimental results are shown in Table two and Figure two.

[0036] Table two: the influence of single material and combination on the TEWL value

[0037]

[0038]

[0039] According to the experimental data in Table two, the combination of 0.1% of the Antarctic haircap moss extract, 0.1% of the ectoine and 0.5% of the glycerol glucoside has a more significant effect on reducing the TEWL value, is obviously better than the single material group and the two-material component group, and has a significant difference between groups, so the combination can produce a significant skin barrier repair effect through the synergistic mechanism of combination.

[0040] The combination of 0.00001%-5% of the Antarctic haircap moss extract, 0.00001%-2% of the ectoine and 0.00001-3% of the glycerol glucoside is found to have a significantly better effect than the single use. After variance analysis, the combination of 0.1% of the Antarctic haircap moss extract, 0.1% of the ectoine and 0.5% of the glycerol glucoside has a significant difference (P less than 0.05) with other sample groups, and the specific analysis process and data are omitted.

[0041] The above examples show that the combination of the Antarctic haircap moss extract, the ectoine and the glycerol glucoside provided by the present application has a significant effect on promoting the expression of the filaggrin FLG and reducing the TEWL value, and has a significant synergistic effect among the three. Therefore, the combination provided by the present application plays a very important role in the skin stability, has a good soothing and repairing effect, and can solve a series of skin problems.

[0042] The above embodiments illustrate the technical solutions of the present application, and further researches on the process, formula and application will be carried out subsequently. It should be understood that the above description is only a specific embodiment of the present application, and is not used to limit the present application. Any modification, supplement or similar substitution within the principle range of the present application should be included in the protection range of the present application.

[0043] Although the present application is disclosed as above, the present application is not limited to this. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the present application. Therefore, the protection scope of the present application should be defined by the scope of the claims.

Claims

1. Use of a composition for the manufacture of a preparation for reducing transepidermal water loss, characterized in that, The composition consists of Antarctic hair cap moss extract, ectoine and glycerol glucoside, the concentration of the Antarctic hair cap moss extract is 0.00001%-5%, the concentration of the ectoine is 0.00001%-2%, and the concentration of the glycerol glucoside is 0.00001%-3%.

2. A skin barrier repair composition characterized in that, The composition consists of Antarctic hair cap moss extract, ectoine and glycerol glucoside, the concentration of the Antarctic hair cap moss extract is 0.00001%-5%, the concentration of the ectoine is 0.00001%-2%, and the concentration of the glycerol glucoside is 0.00001%-3%.

3. Use of a composition for preparing a skin barrier repair preparation, the composition consisting of Antarctic hair cap moss extract, ectoine and glycerol glucoside, the concentration of the Antarctic hair cap moss extract is 0.00001%-5%, the concentration of the ectoine is 0.00001%-2%, and the concentration of the glycerol glucoside is 0.00001%-3%.

4. Use according to claim 3, characterized in that, The composition is used for repairing skin barrier by any one or both of promoting barrier protein expression and reducing transepidermal water loss.

5. Use according to claim 4, characterized in that, The barrier protein is filaggrin FLG.

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