Application of a strain of Lactobacillus salivarius in fermented feed

Abstract

The present invention discloses the application of a strain of Lactobacillus salivarius in fermented feed. The Lactobacillus salivarius of the present invention is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 20700. The feed fermented by the Lactobacillus salivarius of the present invention has improved palatability and nutritional value, which helps to improve production performance and plays an important role in promoting growth, converting feed, and improving body immunity. The fermented feed prepared by the Lactobacillus salivarius of the present invention can be stored at room temperature and still has 10% of the nutritional value after 150 days. 6 A live bacteria count above CFU / g can effectively improve the growth performance of broilers and enhance their immunity.

Description

Technical Field

[0001] The invention relates to application of a strain of Lactobacillus salivarius in fermented feed, belonging to the field of biotechnology. Background Art

[0002] In recent years, the poultry industry has developed rapidly. Enhancing the disease resistance of broilers and improving their growth performance are important issues in the development of the poultry industry. Since the 1950s, the addition of antibiotics to animal diets has significantly promoted animal production and made a significant contribution to the development of intensive animal husbandry. However, as time goes by, the harm of adding antibiotics to feed has become increasingly apparent and has received widespread attention from society. It is now an internationally recognized food safety standard that antibiotics should not be added to broiler chickens. People have begun to seek other alternatives and alternative technologies to ensure that the efficiency and benefits of poultry production are not affected.

[0003] Probiotic fermented feed technology is a new feed technology that has recently grown up with many advantages. After probiotic fermentation, the feed contains more active probiotic bacteria, various enzymes, metabolites at all levels, multiple vitamins, protein decomposition products, active small peptides, amino acids, antibacterial substances, immune enhancing factors, growth promoting factors, etc., which promote growth and maintain the balance of animal intestinal flora. It is an ecologically healthy feed production technology.

[0004] However, the number of viable bacteria in the currently prepared probiotic fermented feed is relatively small, and the growth-promoting effect on broilers is relatively poor. Therefore, there is an urgent need for a probiotic fermented feed with a high number of viable bacteria and a good growth-promoting effect on broilers. Summary of the invention

[0005] In order to solve the above problems, the present invention provides an application of a strain of Lactobacillus salivarius in fermented feed. The number of live bacteria in the feed fermented by the Lactobacillus salivarius of the present invention can reach 10 9 CFU / g, which can effectively improve the growth performance of broilers and enhance the immunity of broilers.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The invention provides application of Lactobacillus salivarius in improving growth performance and / or immunity of poultry; the Lactobacillus salivarius is deposited in China General Microbiological Culture Collection Center with a deposit number of CGMCC No.20700.

[0008] The present invention also provides a fermented feed, which comprises the following raw materials: Lactobacillus salivarius bacterial liquid, complete feed, carbon source and nitrogen source; the volume mass ratio of the Lactobacillus salivarius bacterial liquid to the complete feed is (3-4) L:10 kg; the number of live Lactobacillus salivarius in the Lactobacillus salivarius bacterial liquid is (1-9)×10 8 CFU / mL; the Lactobacillus salivarius is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No.20700;

[0009] The mass ratio of the complete feed, carbon source and nitrogen source is 100:(8-16):(4-8).

[0010] Preferably, the culture medium for preparing the Lactobacillus salivarius bacterial solution comprises the following components in concentration: peptone 12 g / L, beef extract 8 g / L, yeast extract 4 g / L, dipotassium hydrogen phosphate 2 g / L, triammonium citrate 2 g / L, sodium acetate 5 g / L, magnesium sulfate 0.58 g / L, manganese sulfate 0.07 g / L and Tween 80 1 mL / L.

[0011] Preferably, the carbon source includes glucose and brown sugar; the nitrogen source includes beef meal and peptone.

[0012] The present invention also provides a method for preparing the fermented feed described in the above technical solution, comprising the following steps:

[0013] After mixing the prepared raw materials, adjusting the water content to 30% to 35%, and fermenting at room temperature for 48 to 64 hours to obtain the fermented feed;

[0014] The fermented feed is dried at 30-37° C. to a moisture content of 8%-10% to obtain the fermented feed for storage.

[0015] Preferably, the fermentation temperature is 25-30°C.

[0016] Preferably, the fermentation method comprises: placing the mixed prepared raw materials into a feed breathing bag for room temperature fermentation.

[0017] Preferably, after the raw materials are mixed, the moisture content of the mixed raw materials is adjusted to 30% to 35%.

[0018] The present invention also provides the use of the fermented feed described in the above technical solution or the fermented feed prepared by the method described in the above technical solution in improving the growth performance and / or improving the immunity of poultry.

[0019] The present invention also provides a method for improving the growth performance and / or immunity of poultry, comprising:

[0020] Mixing the fermented feed with the diet and feeding the mixture to the poultry; the fermented feed is the fermented feed described in the above technical solution or the fermented feed prepared by the method described in the above technical solution;

[0021] The mass ratio of the fermented feed to the daily diet is (5-10):100.

[0022] Beneficial effects:

[0023] The present invention provides the use of Lactobacillus salivarius in improving the growth performance and / or immunity of poultry; the Lactobacillus salivarius is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No. 20700. The feed fermented by the Lactobacillus salivarius of the present invention has improved palatability and nutritional value, helps to improve production performance, and plays an important role in promoting growth, converting feed, improving body immunity, etc. The fermented feed prepared by the Lactobacillus salivarius of the present invention can be stored at room temperature and still has 10 6 A live bacteria count above CFU / g can effectively improve the growth performance of broilers and enhance their immunity.

[0024] Biological deposit information

[0025] Lactobacillus salivarius, with the Latin name Lactobacillus salivarius, was deposited at the General Microbiology Center of China Culture Collection Administration on September 23, 2020. The deposit address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 20700. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0027] Figure 1 is the number of viable bacteria in different fermented feeds;

[0028] Figure 2 The average daily weight gain results of different groups;

[0029] Figure 3 The average daily feed intake results of different groups;

[0030] Figure 4 The results of feed-to-weight ratio of different groups;

[0031] Figure 5 Shown are the immune index results of different groups. DETAILED DESCRIPTION

[0032] The invention provides application of Lactobacillus salivarius in improving growth performance and / or immunity of poultry; the Lactobacillus salivarius is deposited in China General Microbiological Culture Collection Center with a deposit number of CGMCC No.20700.

[0033] The Lactobacillus salivarius described in the present invention is a Gram-positive bacillus, has no catalase and oxidase, can produce lactic acid and bacteriocins, and can improve the health level of the host, such as regulating intestinal microbial flora, antibacterial, stimulating protective immune response, and making the intestines acidified appropriately. After the feed is fermented by the Lactobacillus salivarius described in the present invention, the palatability and nutritional value are improved, which helps to improve production performance and plays an important role in promoting growth, converting feed, and improving the body's immunity. The fermented feed prepared by the Lactobacillus salivarius described in the present invention can be stored at room temperature and still has 10 6 The number of viable bacteria above CFU / g.

[0034] The invention provides a fermented feed, which comprises the following raw materials: a Lactobacillus salivarius bacterial liquid, a complete feed, a carbon source and a nitrogen source; the volume mass ratio of the Lactobacillus salivarius bacterial liquid to the complete feed is (3-4) L:10 kg; the number of live Lactobacillus salivarius in the Lactobacillus salivarius bacterial liquid is (1-9)×10 8 CFU / mL; the Lactobacillus salivarius is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No.20700;

[0035] The mass ratio of the complete feed, carbon source and nitrogen source is 100:(8-16):(4-8).

[0036] The raw materials for preparing the fermented feed of the present invention include complete feed. The complete feed of the present invention is preferably purchased from broiler early compound feed 610 of Kunming Guanghong Feed Co., Ltd.

[0037] The raw materials for preparing the fermented feed of the present invention include Lactobacillus salivarius bacterial liquid.

[0038] In the present invention, the culture medium for preparing the Lactobacillus salivarius bacterial solution preferably includes the following components in concentration: peptone 12g / L, beef extract 8g / L, yeast extract 4g / L, dipotassium hydrogen phosphate 2g / L, triammonium citrate 2g / L, sodium acetate 5g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.07g / L and Tween 80 1mL / L; the pH value of the culture medium is preferably 6.5.

[0039] The present invention preferably further provides a method for preparing the Lactobacillus salivarius bacterial liquid, comprising: inoculating Lactobacillus salivarius into the culture medium described in the above technical solution, and culturing on a shaking table at 37° C. for 24 hours to obtain the Lactobacillus salivarius bacterial liquid.

[0040] The number of viable Lactobacillus salivarius in the Lactobacillus salivarius bacterial solution of the present invention is (1-9)×10 8 CFU / L, preferably 1×10 8 CFU / L; the volume mass ratio of the Lactobacillus salivarius liquid to the complete feed is (3-4) L:10 kg, preferably 3 L:10 kg.

[0041] The raw materials for preparing the fermented feed of the present invention include a carbon source, and the carbon source preferably includes glucose and brown sugar; the mass ratio of the glucose to the complete feed is preferably (4-8):100, further preferably (4-6):100, and more preferably 4.5:100; the mass ratio of the brown sugar to the complete feed is preferably (4-8):100, further preferably (4-6):100, and more preferably 4.5:100.

[0042] The raw materials for preparing the fermented feed of the present invention include a nitrogen source; the nitrogen source preferably includes beef powder and peptone; the mass ratio of the beef powder to the complete feed is preferably (2-4):100, further preferably (3-4):100, and more preferably 4:100; the mass ratio of the peptone to the complete feed is preferably (2-4):100, further preferably (3-4):100, and more preferably 4:100.

[0043] The fermented feed provided by the present invention uses Lactobacillus salivarius with a preservation number of CGMCC No. 20700 as a probiotic, and the raw materials used are complete feed supplemented with carbon source and nitrogen source. It has sufficient nutrition and simple process, but can make Lactobacillus salivarius reach a high number of live bacteria. The live bacteria count after fermentation can reach up to 10 10 CFU / g.

[0044] The present invention also provides a method for preparing the fermented feed described in the above technical solution, comprising the following steps:

[0045] After mixing the prepared raw materials, adjusting the water content to 30% to 35%, and fermenting for 48 to 64 hours to obtain the fermented feed;

[0046] The fermented feed is dried at 30-37° C. to a moisture content of 8%-10% to obtain the fermented feed for storage.

[0047] The invention mixes the prepared raw materials and ferments them for 48 to 64 hours to obtain fermented materials.

[0048] In the present invention, after the raw materials are mixed, the water content of the raw materials is preferably adjusted to 30% to 35%, more preferably 30%.

[0049] In the present invention, the fermentation temperature is preferably 25-30°C, more preferably 30°C; the fermentation time is 48-64h, preferably 48h; the fermentation method preferably includes: putting the mixed raw materials into a feed breathing bag for fermentation. The feed breathing bag of the present invention preferably includes a feed breathing bag with a one-way breathing valve.

[0050] The present invention adopts a feed breathing bag for fermentation and cultivation, so that Lactobacillus salivarius has an independent fermentation environment and will not be polluted by the external environment such as miscellaneous bacteria, which not only ensures the activity of the microorganisms, but also ensures that the product can be stored and transported for a long time. This innovative technology of packaging first and fermenting later cleverly solves the problems of heat dissipation, packaging, storage, transportation, stability, etc. of microbial solid fermentation, and greatly reduces the production cost of microbial fermented feed.

[0051] After obtaining the fermented material, the present invention dries the fermented material at 30-37° C. to a moisture content of 8%-10% to obtain the fermented feed.

[0052] In the present invention, the drying temperature is 30-37°C, preferably 30-35°C, more preferably 30°C; the drying method preferably includes boiling drying; the moisture content of the stored fermented feed is 8%-10%, preferably 10%. The present invention can not only increase the storage time of the fermented feed, but also prolong the activity of Lactobacillus salivarius by drying the fermented material to a suitable moisture content at a suitable temperature.

[0053] The present invention also provides the use of the fermented feed described in the above technical solution or the fermented feed prepared by the method described in the above technical solution in improving the growth performance and / or improving the immunity of poultry.

[0054] The fermented feed provided by the invention has the effects of improving animal growth performance, enhancing body immunity and the like, and is expected to replace antibiotics and become a new feed additive.

[0055] The present invention also provides a method for improving the growth performance and / or immunity of poultry, comprising:

[0056] Mixing the fermented feed with the diet and feeding the mixture to the poultry; the fermented feed is the fermented feed described in the above technical solution or the fermented feed prepared by the method described in the above technical solution;

[0057] The mass ratio of the fermented feed to the daily diet is (5-10):100, preferably 10:100.

[0058] Below in conjunction with specific embodiment, further elaborate the present invention.But these embodiments are limited to illustrate the present invention and are not used in limiting the protection scope of the present invention.The experimental method in the following examples is conventional method unless otherwise specified.The materials used, reagents etc. in the following examples are all available from commercial sources unless otherwise specified.The Lactobacillus salivarius deposit number in the embodiment is CGMCC No.20700.

[0059] Example 1

[0060] A fermented feed is prepared by the following method:

[0061] 1) Weigh 12 g of tryptone, 8 g of beef extract, 4 g of yeast extract, 2 g of dipotassium hydrogen phosphate, 2 g of triammonium citrate, 5 g of sodium acetate, 0.58 g of magnesium sulfate, 0.07 g of manganese sulfate and 1 mL of Tween 80, put them into a conical flask, add deionized water to make the volume to 1 L, adjust the pH to 6.5, and sterilize at 121°C for 30 min; inoculate 2 mL of preserved Lactobacillus salivarius, and culture at 37°C in a shaking incubator for 24 h to obtain a Lactobacillus salivarius bacterial solution (effective viable count of 10 8 CFU / mL); the Lactobacillus salivarius is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No.20700;

[0062] 2) preparing 3 L of Lactobacillus salivarius liquid according to the method described in step 1);

[0063] 3) 450 g of glucose, 450 g of brown sugar, 400 g of beef powder, 400 g of peptone and 1 L of distilled water were mixed, sterilized at 115° C. for 20 min under high pressure, cooled, and mixed with 3 L of the Lactobacillus salivarius liquid and 10 kg of complete feed (pre-broiler compound feed 610 purchased from Kunming Guanghong Feed Co., Ltd.) to obtain a mixed feed;

[0064] 4) placing the mixed material described in step 3) into a feed breathing bag, adjusting the moisture content to 35±3%, and fermenting and culturing the mixture in the feed breathing bag at 30° C. for 48 hours to obtain a fermented feed;

[0065] The obtained fermented feed was placed at 30°C for normal temperature boiling drying to prepare a fermented feed for storage with a moisture content of 10% (referred to as fermented Lactobacillus salivarius feed).

[0066] Comparative Example 1

[0067] A fermented feed similar to the embodiment, except that Lactobacillus salivarius is replaced by Bacillus licheniformis; the Bacillus licheniformis is purchased from China General Microbiological Culture Collection Center, with a collection number of CGMCC No.21267.

[0068] Comparative Example 2

[0069] A fermented feed similar to the embodiment, the difference is that Lactobacillus salivarius is replaced by Lactococcus lactis; the Lactococcus lactis is purchased from China General Microbiological Culture Collection Center, with a collection number of CGMCC No.20699.

[0070] Comparative Example 3

[0071] A fermented feed similar to the embodiment, the difference is that Lactobacillus salivarius is replaced by Lactobacillus plantarum; the Lactobacillus plantarum is purchased from China General Microbiological Culture Collection Center, with a collection number of CGMCC No.21266.

[0072] Comparative application example 1

[0073] The fermented feeds prepared in Example 1 and Comparative Examples 1 to 3 were stored at room temperature of 25°C, and samples were taken on the 0th (i.e., the day of storage), 30th, 60th, 90th, 120th, and 150th days. The number of viable bacteria in different fermented feeds was checked by plate spreading method. The results are shown in Table 1. Figure 1 and Table 1.

[0074] Table 1 Number of viable bacteria in different fermented feeds (CFU / g)

[0075] time Lactobacillus salivarius Lactococcus lactis Lactobacillus plantarum Bacillus licheniformis 0d <![CDATA[1.3×10 10 CFU / g]]> <![CDATA[9×10 9 CFU / g]]> <![CDATA[1.2×10 10 CFU / g]]> <![CDATA[9.6×10 9 CFU / g]]> 30d <![CDATA[1.6×10 9 CFU / g]]> <![CDATA[6×10 9 CFU / g]]> <![CDATA[3×10 9 CFU / g]]> <![CDATA[5.6×10 9 CFU / g]]> 60d <![CDATA[9×10 8 CFU / g]]> <![CDATA[1×10 9 CFU / g]]> <![CDATA[9×10 8 CFU / g]]> <![CDATA[8×10 8 CFU / g]]> 90d <![CDATA[8.1×10 8 CFU / g]]> <![CDATA[6.5×10 8 CFU / g]]> <![CDATA[2.2×10 8 CFU / g]]> <![CDATA[7.5×10 8 CFU / g]]> 120d <![CDATA[6.5×10 8 CFU / g]]> <![CDATA[8×10 7 CFU / g]]> <![CDATA[2.6×10 7 CFU / g]]> <![CDATA[5.3×10 8 CFU / g]]> 150d <![CDATA[5.6×10 6 CFU / g]]> <![CDATA[6.2×10 5 CFU / g]]> <![CDATA[8.4×10 4 CFU / g]]> <![CDATA[3×10 6 CFU / g]]>

[0076] from Figure 1 As can be seen from Table 1, the number of viable bacteria of the four probiotics was maintained at 10 before 120 days. 7 ~10 10 CFU / g, and the number of viable bacteria of Lactobacillus salivarius and Bacillus licheniformis remained at 10 after 120 days. 8 ~10 9 CFU / g, while Lactococcus lactis has dropped to 10 8 CFU / g, Lactobacillus plantarum has dropped to 10 7 CFU / g. At 150 days, the number of viable Lactobacillus salivarius can still maintain 10 6 CFU / g, and is higher than that of Bacillus licheniformis, and much higher than that of Lactococcus lactis and Lactobacillus plantarum. It is proved that the fermented feed prepared by using Lactobacillus salivarius of the present invention has a better storage effect, and the probiotics with higher activity have a more favorable effect on the host.

[0077] Comparative Application Example 2

[0078] Growth of broilers fed with fermented Lactobacillus salivarius feed

[0079] 1. Select 60 one-day-old broilers and randomly divide them into 4 groups, with 15 chickens in each group. Each group of broilers was treated as follows:

[0080] 1) Negative control group: fed with complete feed;

[0081] 2) Positive control group: fed with complete feed supplemented with chlortetracycline (2 g / kg); the mass ratio of chlortetracycline to complete feed was 2:1000;

[0082] 3) Low concentration group: fed with complete feed supplemented with low concentration fermented feed; the mass ratio of the low concentration fermented feed to the complete feed was 10:100;

[0083] 4) High concentration group: fed with complete feed supplemented with high concentration fermented feed; the mass ratio of the high concentration fermented feed to the complete feed was 10:100;

[0084] The preparation method of the low-concentration fermented feed is as follows: after the fermented feed is prepared according to the method described in Example 1, 10 g of the feed is added into 90 mL of sterile water for coating and detection of the number of viable bacteria, and the test is performed once every other day until the number of viable bacteria reaches 1×10 6 CFU / g;

[0085] The preparation method of the high-concentration fermented feed is as follows: after the fermented feed is prepared according to the method described in Example 1, 10 g of the feed is added into 90 mL of sterile water for coating and detection of the number of viable bacteria, and the test is performed once every other day until the number of viable bacteria reaches 1×10 7 CFU / g.

[0086] The test period is 3 weeks. Before the test begins, the test site is fumigated and disinfected, and the test begins after ventilation and drying. In the early stage of the test, the first week is the brooding period, and the temperature and humidity in the chicken house are strictly controlled. During the test, the immunization program of broilers is carried out in accordance with the daily management procedures of the farm. Feed and water are freely available. The health status of the chickens is carefully observed every day, and sick and weak chickens are eliminated in time. The weight of broilers is weighed on an empty stomach on the first, seventh, 14th and 21st days of the test. During the test, the feed intake of each group of broilers is accurately recorded, and the average daily feed intake, average daily weight gain, and feed-to-weight ratio are calculated.

[0087] Average daily weight gain = (final weight - initial weight) / number of test days;

[0088] Average daily feed intake = total feed intake / number of experimental days;

[0089] Feed-to-weight ratio = average daily feed intake / average daily weight gain;

[0090] Results Figures 2 to 4 and Tables 2 to 4.

[0091] Table 2 Average daily weight gain results of different groups (g)

[0092] time Blank control Chlortetracycline <![CDATA[Lactobacillus salivarius 10 6 CFU / g]]> <![CDATA[Lactobacillus salivarius 10 7 CFU / g]]> 1～7d 2.6±0.4g 3.73±0.7g 4.17±0.17g 3.94±0.3g 8～14d 6.15±0.75g 5.76±0.5g 7.29±0.29g 6.80±0.3g 15～21d 6.42±0.3g 6.81±0.4g 7.65±0.5g 8.40±0.4g

[0093] Table 3 Average daily feed intake of different groups (g)

[0094] time Blank control Chlortetracycline <![CDATA[Lactobacillus salivarius 10 6 CFU / g]]> <![CDATA[Lactobacillus salivarius 10 7 CFU / g]]> 1～7d 9.52±1.3g 9.51±2.3g 13.34±0.3g 13.32±1.6g 8～14d 16.05±0.6g 15.21±1.2g 16.70±0.9g 15.23±1.3g 15～21d 18.10±1.61g 18.18±1.6g 21.34±0.5g 19.07±1.5g

[0095] Table 4 Results of feed weight ratio of different groups

[0096] time Blank control Chlortetracycline <![CDATA[Lactobacillus salivarius 10 6 CFU / g]]> <![CDATA[Lactobacillus salivarius 10 7 CFU / g]]> 1～7d 3.66±0.62 2.55±0.69 3.2±1.5 3.38±0.45 8～14d 2.61±0.51 2.64±1.65 2.29±1.2 2.24±0.51 15～21d 2.82±1.3 2.67±1.54 2.79±0.68 2.27±0.94

[0097] Depend on Figures 2 to 4 As shown in Tables 2 to 4, the fermented Lactobacillus salivarius feed of the present invention performed better than the negative control and the chlortetracycline control in average daily weight gain, among which the high concentration of 10 7 The average daily weight gain of CFU / g of Lactobacillus salivarius was 1.59g higher than that of the chlortetracycline control in the third week, and about 1.98g higher than that of the negative control. The average daily feed intake of Lactobacillus salivarius feed was also higher than that of chlortetracycline and the negative control. Among them, the feed-to-weight ratio is one of the important indicators reflecting the growth performance of broilers. The feed-to-weight ratio of Lactobacillus salivarius feed is lower than that of chlortetracycline and the negative control, showing a higher feed utilization efficiency.

[0098] 2. On the 21st day of the experiment, 3 broilers were randomly selected from each replicate, 5 mL of blood was collected from the neck vein, centrifuged at 3500 r / min for 6 min, and the upper serum was taken into an EP tube for the detection of serum immune indicators, including immunoglobulin A (IgA), immunoglobulin G (IgG), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10); the results are shown in Figure 5 and Table 5.

[0099] Table 5 Immune index test results of different groups

[0100]

[0101]

[0102] Depend on Figure 5As shown in Table 5, Lactobacillus salivarius feed has a significant effect on improving the immune ability of broilers. IgA and IgG are the most important antibodies in the body, with antiviral, virus neutralization, antibacterial and immunomodulatory functions. The IgA and IgG levels in the experimental group fed with Lactobacillus salivarius feed were higher than those in the negative control group, reaching the level of the chlortetracycline group, indicating that Lactobacillus salivarius has a good effect in enhancing host immune antibodies. IL-6 and TNF-α tumor factors are pro-inflammatory factors, and IL-10 is an anti-inflammatory factor. Compared with the negative control group, the pro-inflammatory factor (IL-6, TNF-α) content of the Lactobacillus salivarius group and the chlortetracycline group is decreasing, while the anti-inflammatory factor (IL-10) content is increasing, indicating that feeding Lactobacillus salivarius and chlortetracycline can reduce the possibility of inflammatory symptoms in broilers, improve the immunity of broilers, and also explain that Lactobacillus salivarius and chlortetracycline have similar effects and can replace antibiotics as feed additives.

[0103] In summary, the application of the strain of Lactobacillus salivarius in fermented feed has the effects of improving animal growth performance, enhancing body immunity and the like, and is expected to replace antibiotics and become a new feed additive.

[0104] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Lactobacillus salivarius ( Lactobacillus salivarius ) strain in the preparation of fermented feed for improving poultry growth performance and / or improving poultry immunity; the Lactobacillus salivarius strain is deposited in the China General Microbiological Culture Collection Center with the deposit number CGMCC No.20700.

2. The use according to claim 1, It is characterized in that The fermented feed comprises the following raw materials: Lactobacillus salivarius ( Lactobacillus salivarius ) strain bacterial liquid, complete feed, carbon source and nitrogen source; the volume mass ratio of the Lactobacillus salivarius bacterial liquid and the complete feed is (3-4) L:10 kg; the number of viable Lactobacillus salivarius in the Lactobacillus salivarius bacterial liquid is (1-9)×10 8 CFU / mL; the Lactobacillus salivarius strain is deposited in the China General Microbiological Culture Collection Center with a deposit number of CGMCC No.20700; The mass ratio of the complete feed, carbon source and nitrogen source is 100:(8-16):(4-8).

3. The use according to claim 2, It is characterized in that The culture medium for preparing the bacterial liquid of the Lactobacillus salivarius strain includes the following components in concentrations: 12 g / L peptone, 8 g / L beef extract, 4 g / L yeast extract, 2 g / L dipotassium hydrogen phosphate, 2 g / L triammonium citrate, 5 g / L sodium acetate, 0.58 g / L magnesium sulfate, 0.07 g / L manganese sulfate and 1 mL / L Tween 80.

4. The use according to claim 2, It is characterized in that The carbon source includes glucose and brown sugar; the nitrogen source includes beef powder and peptone.

5. The use according to any one of claims 1 to 4, It is characterized in that The method for preparing the fermented feed is characterized by comprising the following steps: After mixing the prepared raw materials, adjusting the water content to 30% to 35%, and fermenting for 48 to 64 hours to obtain the fermented feed; The fermented feed is dried at 30-37° C. to a moisture content of 8%-10% to obtain the fermented feed.

6. The use according to claim 5, It is characterized in that The fermentation temperature is 25-30°C.

7. The use according to claim 6, It is characterized in that The fermentation method comprises: putting the mixed prepared raw materials into a feed breathing bag for fermentation.

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