Antibacterial material and respiratory humidifier oxygen tube using the same

Abstract

Disclosed is an antibacterial material, including polyhexanide, guanidinized polymer and hydroxycarboxylic acid. In addition, disclosed are an antibacterial material solution, an antibacterial treatment method for a PVC oxygen inhalation tube of a respiratory humidifier, and a PVC oxygen inhalation tube of a respiratory humidifier. The present invention uses an antibacterial material of a specific composition, and obtains a PVC oxygen inhalation tube of a respiratory humidifier after antibacterial treatment. Compared with the prior art, the PVC oxygen inhalation tube of a respiratory humidifier has better antibacterial performance against Staphylococcus aureus and Escherichia coli.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices, and in particular relates to an antibacterial material and an oxygen inhalation tube of a respiratory humidifier using the same. Background Art

[0002] High-flow respiratory humidification therapy refers to a treatment method that continuously provides patients with high-flow (8-80L / min) inhaled gas with adjustable and relatively constant oxygen concentration (21%-100%), temperature (31-37°C) and humidity through a high-flow dedicated nasal plug or other patient interface. Compared with non-invasive ventilators, its significant advantages lie in comfort, tolerance and compliance. For patients with mild to moderate hypoxemia, a high-flow respiratory humidification therapy device is recommended as the first choice for treatment.

[0003] Similar to ordinary oxygen therapy, high-flow respiratory humidification therapy also adds distilled water to the oxygen humidification bottle for humidification, and then connects the flow meter to the oxygen inhalation tube, but its effect of improving oxygenation is better. However, many open operations such as the infusion of humidification liquid and the storage of humidification bottles may cause contamination of the oxygen inhalation device, especially the PVC oxygen inhalation tube, which may make the patient susceptible to infection.

[0004] Therefore, there is an urgent need to provide a PVC oxygen tube for a respiratory humidifier with better antibacterial properties in view of the above-mentioned defects of the prior art. Summary of the invention

[0005] The purpose of the present invention is to provide a PVC oxygen absorption tube for a respiratory humidifier with better antibacterial property.

[0006] To achieve the purpose of the invention, on the one hand, the present invention adopts the following technical solution: an antibacterial material, including polyhexanide, guanidinized polymer and hydroxycarboxylic acid.

[0007] According to the antibacterial material of the present invention, the guanylated polymer is selected from guanylated polyethyleneimine.

[0008] According to the antibacterial material of the present invention, the weight average molecular weight M of the guanidinium polyethyleneimine is w =(1.5-2)×10 4 , number average molecular weight M n =(8-10)×10 3 , polydispersity index PDI = 1.6-2.2.

[0009] In a specific embodiment, the weight average molecular weight M of the guanidinized polyethyleneimine is w =1.75×10 4 , number average molecular weight M n =9.3×10 3 , polydispersity index PDI = 1.9.

[0010] According to the antibacterial material of the present invention, the hydroxycarboxylic acid is selected from tartaric acid.

[0011] According to the antibacterial material of the present invention, the weight ratio of polyhexanide, guanidinized polymer and hydroxycarboxylic acid is (3-5):(1-3):(3-5).

[0012] In a specific embodiment, the weight ratio of polyhexanide, guanidinized polymer and hydroxycarboxylic acid is 4:2:4.

[0013] In another aspect, the present invention provides an antibacterial material solution, which consists of the antibacterial material described above and below and anhydrous ethanol.

[0014] According to the antibacterial material solution of the present invention, the concentration of the antibacterial material mentioned above and below is (0.5-4)% g / mL.

[0015] In a specific embodiment, the concentration of the antibacterial material described above and below is 2% g / mL.

[0016] In another aspect, the present invention provides a method for antibacterial treatment of a PVC oxygen tube of a respiratory humidifier, comprising: immersing the PVC oxygen tube in the antibacterial material solution described above for a certain period of time.

[0017] According to the antibacterial treatment method of the present invention, the time is 1-10 minutes.

[0018] In a specific embodiment, the time is 5 minutes.

[0019] In the last aspect, the present invention provides a PVC oxygen absorption tube for a respiratory humidifier, comprising a main body and an antibacterial layer; wherein the antibacterial layer is obtained by the antibacterial treatment method described above.

[0020] The inventors found that a respiratory humidifier PVC oxygen inhalation tube was obtained by using an antibacterial material of a specific composition and undergoing antibacterial treatment. Compared with the prior art, the respiratory humidifier PVC oxygen inhalation tube has better antibacterial performance against Staphylococcus aureus and Escherichia coli. DETAILED DESCRIPTION

[0021] The present invention is further described below in conjunction with specific implementation methods.

[0022] It should be understood that the specific embodiments of the present invention are only used to illustrate the spirit and principles of the present invention, and are not used to limit the scope of the present invention. In addition, it should be understood that after reading the content of the present invention, those skilled in the art can make various changes, substitutions, deletions, corrections or adjustments to the technical solutions of the present invention, and these equivalent technical solutions also fall within the scope defined by the claims of the present invention.

[0023] In the present invention, unless otherwise specified, all parts are parts by weight; all percentages are percentages by weight.

[0024] Example 1

[0025] Polyethyleneimine (number average molecular weight M n =600) and guanidine hydrochloride were added to the flask in a mass ratio of 1.3:1, and the temperature was raised to 90°C under nitrogen atmosphere and stirring conditions, and the reaction was continued for 2 hours; the temperature was further raised to 160°C and the reaction was continued for 4 hours; dilute hydrochloric acid was used to absorb the by-product ammonia in the reaction process. After the reaction was completed, a light yellow viscous liquid was obtained, which was cooled to room temperature and dried to obtain a guanidine polyethyleneimine product. The weight average molecular weight M of guanidine polyethyleneimine w =1.75×10 4 , number average molecular weight M n =9.3×10 3 , polydispersity index PDI = 1.9.

[0026] Example 2

[0027] According to the formula, 8 g of polyhexamethylenebiguanide (PAPB), 4 g of guanylated polyethyleneimine and 8 g of tartaric acid were dissolved in 1 L of ethanol to obtain an antibacterial material solution.

[0028] Example 3

[0029] The PVC oxygen absorption tube was ultrasonically cleaned in anhydrous ethanol for 30 minutes, dried at room temperature, and then immersed in the antibacterial material solution of Example 2 for 5 minutes, dried at room temperature, and cleaned with ultrapure water for 3 times.

[0030] Comparative Example 1

[0031] According to the formula, 10 g of polyhexamethylenebiguanide (PAPB) and 10 g of tartaric acid were dissolved in 1 L of ethanol to obtain an antibacterial material solution.

[0032] The PVC oxygen absorption tube was ultrasonically cleaned in anhydrous ethanol for 30 minutes. After drying at room temperature, it was immersed in the antibacterial material solution for 5 minutes. It was dried at room temperature and washed with ultrapure water for 3 times.

[0033] Antibacterial performance test

[0034] Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 25922) were inoculated on LB solid medium and incubated at 37°C for 24 hours. The selected monoclonal strain was transferred to 25 mL of liquid medium and cultured on a constant temperature shaker for 12 hours. The liquid medium containing bacteria was centrifuged at 3000 rpm for 10 minutes, the supernatant was removed, and the bacterial concentration was adjusted to 1×10 6CFU / mL.

[0035] The number of live bacteria in the sample was counted using the JIS Z 2801 standard. The PVC oxygen tube samples of Example 3 and Comparative Example 1 were placed in a 12-well plate, 25 μL of the above bacterial suspension was added to the center area of ​​the surface, and covered with a PE film (1×1 cm) to evenly spread the bacterial liquid on the surface of the PVC oxygen tube. The plate was incubated at 37°C for 24 hours, and then 3 mL of PBS solution was added and ultrasonicated for 2 minutes to de-adhere the bacteria on the surface. The PBS solution containing bacteria was diluted by a certain multiple and applied to the solid culture medium. After overnight incubation at 37°C, the bacterial colonies formed on the surface of the solid culture medium were counted and counted.

[0036] The results are shown in Table 1.

[0037] Table 1

[0038] sample Staphylococcus aureus Escherichia coli Example 3 98.2％ 99.3％ Comparative Example 1 51.6％ 67.5％

[0039] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.

Claims

1. An antibacterial material, characterized in that: It is composed of polyhexanide, guanidinized polymer and hydroxycarboxylic acid; in, The guanylated polymer is selected from guanylated polyethyleneimine; the weight average molecular weight M of the guanylated polyethyleneimine is w =(1.5-2)×10 4 , number average molecular weight M n =(8-10)×10 3 , polydispersity index PDI = 1.6-2.2; The hydroxycarboxylic acid is selected from tartaric acid; The weight ratio of polyhexanide, guanidinized polymer and hydroxycarboxylic acid is (3-5):(1-3):(3-5).

2. An antibacterial material solution, characterized in that: The method comprises the antibacterial material according to claim 1 and anhydrous ethanol.

3. The antibacterial material solution according to claim 2, wherein: The concentration of antibacterial material is (0.5-4)%.

4. A method for antibacterial treatment of a PVC oxygen tube of a respiratory humidifier, comprising: The PVC oxygen absorption tube is immersed in the antibacterial material solution according to claim 2 or 3 for a certain period of time.

5. The antibacterial treatment method according to claim 4, wherein: The time is 1-10 min.

6. A PVC oxygen tube for a respiratory humidifier, comprising a body and an antibacterial layer; characterized in that: The antibacterial layer is obtained by the antibacterial treatment method according to claim 4 or 5.