A highly stable and easily absorbed codonopsis gel mask and preparation method thereof

Abstract

The present invention relates to the technical field of skin care products, and in particular to a highly stable and easily absorbable codonopsis gel mask and a preparation method thereof. The present invention adopts CBSP-CP as a gel matrix and combines it with a codonopsis extract to prepare a gel mask, which has better stability, better conditioning and repairing effect, and stronger skin tightening effect than gel masks prepared with different types of matrices such as traditional Carbopol 940, improved Carbopol ETD 2020, and polyacrylate cross-linked polymer-6 (Zen).

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to a highly stable and easily absorbable codonopsis pilosula gel mask, which has better stability, better conditioning and repairing effect, and stronger skin tightening effect. Background Art

[0002] Aging is a process in which the body gradually loses its functions and moves toward death. As the largest organ of the body, the skin is a defensive barrier between the body and the external environment, and it can reflect aging more directly and obviously than other organs. As people's pace of life accelerates, women's life pressure continues to increase and they lack sleep, which makes women's skin condition worse and worse, showing signs of aging prematurely. At the same time, most women have higher and higher requirements for skin condition. Wash-free sleeping masks are popular with consumers because of their overnight wash-free characteristics. However, the current wash-free sleeping masks mainly tend to have simple hydration and moisturizing functions. If you want to give the wash-free sleeping masks the functions of repairing and anti-aging, the wash-free sleeping masks have poor stability, which is not conducive to skin absorption, and the effective ingredients are difficult to penetrate and absorb, resulting in poor skin conditioning and repair effects.

[0003] Skin aging is usually divided into endogenous and exogenous aging. Exogenous aging is mainly caused by environmental factors. Ultraviolet (UV) radiation is the main environmental factor that causes skin aging. Long-term exposure to UV radiation will accelerate aging, which is mainly manifested as surface roughness, reduced water content and elasticity, reduced collagen, and wrinkle formation, which is the so-called skin photoaging (also known as photoaging). Repairing the skin after a day of exposure can timely improve the skin damage caused by UV radiation and slow down the occurrence of aging.

[0004] In recent years, modern cosmetics have gradually exposed more and more potential safety hazards. The international community has gradually attached importance to the environmental protection, safety and naturalness of cosmetics, and has begun to regulate them with relevant laws and regulations. Chinese herbal skin care products have definite beauty and skin care effects, historical heritage, green and natural, safe and reliable; Chinese herbal medicine has thousands of years of clinical application experience in humans, and has accumulated many single products and compound prescriptions with unique effects and significant effects. Cosmetics with added Chinese herbal active ingredients are a type of cosmetics with traditional Chinese characteristics. Combined with the ideas of traditional Chinese medicine, active ingredients extracted from Chinese herbal medicine are added to them, which can not only meet people's needs for skin care and beauty, but also enrich consumers' personalized consumption choices and conform to the fashion trend of "returning to the original ecology".

[0005] Codonopsis pilosula: The dried root of Codonopsis pilosula (Franch.) Nannf., Codonopsis pilosula Nannf.var.modesta (Nannf.) LTShen or Codonopsis tangshen Oliv. of Campanulaceae. Codonopsis pilosula is sweet and neutral in nature. It can nourish the middle qi, quench thirst, strengthen the spleen and lung, nourish blood and produce body fluid. It is used for spleen and lung qi deficiency, fatigue due to poor appetite, cough and asthma, qi and blood deficiency, sallow complexion, palpitations and shortness of breath, thirst due to loss of body fluid, internal heat and thirst, lazy speech and shortness of breath, weakness in the limbs, poor appetite, qi deficiency, qi and body fluid deficiency, qi and blood deficiency, and blood deficiency and sallow complexion. Modern pharmacological studies have shown that Codonopsis pilosula has multiple effects such as protecting gastrointestinal mucosa, anti-ulcer, enhancing body immunity, anti-fatigue, promoting hematopoietic function and anti-tumor. Codonopsis contains complex chemical components. According to literature reports, it mainly contains phenylpropanoid glycosides, coumarins, codonopsis polysaccharides, flavonoids and alkaloids. On January 11, 2018, the National Health and Family Planning Commission included Codonopsis in the list of "medicine and food of the same origin", indicating that Codonopsis will play a greater role in more fields.

[0006] Hyaluronic acid and sodium hyaluronate: Hyaluronic acid, also known as hyaluronic acid, is an acidic mucopolysaccharide. It is an important basic substance for tender skin and is also a component of the human body. It has a special water-retaining effect, and the water-retaining capacity can be up to 100 times its own weight. It is the best moisturizing substance found in nature so far and is called an ideal natural moisturizing factor. It can improve skin nutrition metabolism, make the skin tender, smooth, remove wrinkles, increase elasticity, and prevent aging. While moisturizing, it is also a good transdermal absorption promoter. Used in combination with other nutrients, it can achieve the ideal effect of promoting nutrient absorption. Sodium hyaluronate is the sodium salt of hyaluronic acid, which is more stable and has smaller molecules, and can be more effectively absorbed and penetrated deeper into the skin.

[0007] Vitamin C ethyl ether: It is a very useful vitamin C derivative that does not change color. It is lipophilic and hydrophilic, with a wide range of applications and is commonly used in daily chemicals. It can easily pass through the stratum corneum into the dermis and be decomposed by biological enzymes in the body, thereby exerting the biological effects of vitamin C. It has the functions of promoting collagen proliferation, repairing ultraviolet damage to the skin, and diluting melanin. At the same time, it competitively inhibits tyrosinase and reduces dopamine and dopaquinone, the intermediate products of melanin formation, which has the effect of diluting spots.

[0008] From the perspective of traditional Chinese medicine, the spleen and stomach are the foundation of acquired constitution. If the spleen and stomach are weak, the biochemical deficiency of qi and blood will be insufficient, which will manifest as fatigue, lack of energy and laziness, sallow complexion, and even premature aging. The codonopsis in the facial mask of the present invention can nourish the middle qi and replenish qi, invigorate the spleen and lung, nourish blood and produce body fluid, and promote collagen proliferation with vitamin C ethyl ether, repair the damage of ultraviolet rays to the skin, and dilute melanin. In addition, hyaluronic acid has a good transdermal absorption promoting effect while moisturizing, which can improve the transdermal absorption capacity of active ingredients, further improve the effect of codonopsis in nourishing blood and nourishing the skin, eliminating fatigue, and delaying skin aging.

[0009] Gels refer to thick liquid or semisolid preparations with gel properties made from raw materials and excipients that can form gels (also called gel matrices). Depending on the gel matrix, gels are divided into aqueous gels and oily gels. Aqueous gels are easy to spread, comfortable, non-greasy, easy to wash off, can absorb tissue exudate, do not interfere with the normal physiological function of the skin, have a certain water retention effect, and can promote the transdermal absorption of Chinese medicine extracts. They are widely used in the fields of medicine, daily chemicals, etc., and are deeply favored by patients and consumers. The preparation of Codonopsis extract into aqueous gels expands its application range, increases the drug concentration on the skin at the effective site, and can better achieve its effect of delaying skin aging.

[0010] Carbomer is the most commonly used matrix for aqueous gels. It has important uses such as thickening and suspension. It has the advantages of simple preparation process, good rheological properties, and no irritation to skin and mucous membranes. It is widely used in lotions, creams, and gels, and is a very excellent gel matrix material. Although the preparation process of carbomer gel is simple and can give the gel good rheological properties, its disadvantage is that carbomer has a relatively strong sensitivity to the gel environment, which is manifested in the presence of ions, which will significantly reduce its thickening efficiency; trace amounts of iron or other transition metals will cause the gel to have an irreversible decrease in viscosity, seriously damaging the stability of the system; in addition, high shear, ultraviolet radiation or prolonged stirring will also cause carbomer gel viscosity loss, reducing the viscosity of the gel. The sensitivity of carbomer, especially ion sensitivity, is the main problem in its application. Acid components and mineral ions cannot be added to gels based on carbomer, and certain antibacterial agents need to be avoided, or they can only be used at low concentrations, otherwise the consistency will drop sharply or even disappear. In order to improve the ion resistance of carbomer matrix, Lubrizol® of the United States developed a modified gel matrix, namely Carbopol ETD 2020, but its tolerance to ions is still low. As a gel matrix material with excellent rheological properties, the stability of matrix properties, especially the lack of ion resistance, limits the application of carbomer matrix.

[0011] Explanation of related entries:

[0012] CBSP: cationized Bletilla striata polysaccharide;

[0013] CP: Carbomer;

[0014] CBSP-CP: A complex formed by cationic Bletilla striata polysaccharide and carbomer in a certain proportion, which can be used as a gel matrix.

[0015] CBSP-CP (2:1): A complex formed by cationic Bletilla striata polysaccharide and carbomer in a ratio of 2:1, used as a gelling agent matrix. Summary of the invention

[0016] The purpose of the present invention is to provide a new gel mask to address the shortcomings of existing wash-free sleeping masks, such as poor stability, poor skin penetration during sleep, low transdermal absorption rate of nutrient components, and poor skin conditioning and repair effects. The Codonopsis wash-free gel sleeping mask has better stability, enhances the skin's absorption of effective ingredients, produces better conditioning and repair effects, and has a stronger skin firming effect.

[0017] The purpose of the present invention is to achieve the following technical means:

[0018] Polysaccharides are polysaccharides composed of more than 10 monosaccharides connected by glycosidic bonds. They are widely distributed in nature and are natural macromolecules that make up the cell membranes of higher plants and animals and the cell walls of microorganisms. Studies have found that a variety of polysaccharides have excellent tyrosinase inhibition and antioxidant activity, which is beneficial for inhibiting the production of melanin in the skin and has a significant whitening effect. Polysaccharides also have a significant effect on understanding the homeostasis of skin microorganisms, and have a good effect on inhibiting the growth of harmful bacteria and promoting the growth of beneficial bacteria.

[0019] Bletilla striata polysaccharide is the main component of the traditional Chinese medicine Bletilla striata. It not only has the characteristics of other natural polysaccharides, but also has good rheological properties and good adhesion to tissues such as skin and biological mucosa. It also has pharmacological activity in repairing minor skin wounds. It is an ideal natural daily chemical additive and functional ingredient. Our preliminary experimental studies have found that it is stable in a wide pH range and has good ion resistance. However, as a biological polysaccharide, it has inherent disadvantages such as low solution transparency, slow swelling and hydration rates, and easy stratification of sols. Preliminary experiments have found that when used in combination with other matrices, stratification is also prone to occur after centrifugation and standing, which limits its application.

[0020] The present invention cationizes Bletilla striata polysaccharide, and on this basis combines the cationized Bletilla striata polysaccharide with an anionic polymer, and utilizes the electrostatic interaction between the two to construct a new gel matrix material, in particular, a new gel matrix that is stable, resistant to ions, resistant to complex components of traditional Chinese medicine, has good rheological properties, and has the activity of Bletilla striata polysaccharide. The gel matrix is ​​composed of cationized Bletilla striata polysaccharide (CBSP) and CP that can form a composition with CBSP, wherein CP is Carbopol; by weight, the ratio of cationized Bletilla striata polysaccharide to Carbopol is 2:1.

[0021] Specifically, in the composition, CP is preferably Carbopol ETD 2020 (abbreviated as CP 2020), that is, CBSP and Carbopol ETD 2020 form a composition, and the ratio thereof is CBSP:CP=2:1.

[0022] The preparation method of the gel matrix of the present invention is as follows:

[0023] 0.5% (w / v) CP 2020 was dispersed in 70°C deionized water, stirred at 400 rpm until uniform, and allowed to stand for 12 h to form a uniform, agglomerate-free sol, referred to as phase A;

[0024] 1.0% (w / v) CBSP was dispersed in deionized water at room temperature (25 °C) and stirred at 400 rpm until uniform to form a CBSP solution, referred to as phase B;

[0025] At room temperature (25°C), phase B was slowly added dropwise to phase A, and the pH was adjusted to 6.5 with saturated citric acid solution and triethanolamine. The mixture was freeze-dried to obtain a CBSP-CP composite matrix.

[0026] The present invention provides a highly stable and easily absorbable codonopsis gel facial mask, which comprises: the gel matrix according to claim 1 and a codonopsis extract.

[0027] The Codonopsis gel mask of the present invention further comprises a pH regulator;

[0028] The codonopsis gel mask of the present invention further comprises an additive, characterized in that the additive is selected from one or more of a moisturizer (sodium hyaluronate), a preservative, a transdermal absorption enhancer (menthol), an antioxidant (vitamin C ethyl ether), and a flavor.

[0029] The present invention provides a highly stable and easily absorbable Codonopsis gel mask, wherein the weight ratio of each component is:

[0030] Codonopsis extract is equivalent to 15-55g of medicinal material

[0031] Gel base 0.3-3g

[0032] Sodium hyaluronate 0.1-2g

[0033] Vitamin C ethyl ether 0.5-2g

[0034] Glycerin 0.1-20ml

[0035] 1,2-propylene glycol 0.3 - 4g

[0036] PEG-40 hydrogenated castor oil 0.1-5g

[0037] Phenoxyethanol 0.1-1.5g

[0038] Menthol 0.5-2g

[0039] D-Panthenol 0.1-2g

[0040] Triethanolamine 0.01-2g

[0041] Flavor 0.01-2g

[0042] Appropriate amount of distilled water.

[0043] Furthermore, the weight ratio of each component in the gel mask is:

[0044] Codonopsis extract is equivalent to 20-50g of medicinal material

[0045] Gel base 0.5-2g

[0046] Sodium hyaluronate 0.2-1g

[0047] Vitamin C ethyl ether 0.7-1.5g

[0048] Glycerin 1-15ml

[0049] 1,2-propylene glycol 0.5-3g

[0050] PEG-40 hydrogenated castor oil 0.2-2.5g

[0051] Phenoxyethanol 0.2-1g

[0052] Menthol 0.8-1.5g

[0053] D-Panthenol 0.5-1.5g

[0054] Triethanolamine 0.05-1g

[0055] Flavor 0.05-1.5g

[0056] Appropriate amount of distilled water.

[0057] Furthermore, the weight ratio of each component in the gel mask is:

[0058] Codonopsis extract is equivalent to 15-45g of medicinal material

[0059] Gel base 0.8-1.5g

[0060] Sodium hyaluronate 0.4 - 0.8g

[0061] Vitamin C ethyl ether 0.9-1.2g

[0062] Glycerin 2-11ml

[0063] 1,2-propylene glycol 0.8-1.5g

[0064] PEG-40 hydrogenated castor oil 0.4-1g

[0065] Phenoxyethanol 0.4-0.8g

[0066] Menthol 0.9-1.2g

[0067] D-Panthenol 0.8-1.2g

[0068] Triethanolamine 0.1-0.6g

[0069] Flavor 0.2-1g

[0070] Appropriate amount of distilled water.

[0071] Furthermore, the weight ratio of each component in the gel mask is:

[0072] Codonopsis pilosula extract is equivalent to 45g of medicinal material

[0073] Gel base 1g

[0074] Sodium hyaluronate 0.5 g

[0075] Vitamin C ethyl ether 1g

[0076] Glycerin 10ml

[0077] 1,2 Propylene glycol 1g

[0078] PEG-40 hydrogenated castor oil 0.5g

[0079] Phenoxyethanol 0.5g

[0080] Menthol 1g

[0081] D-Panthenol 1g

[0082] Triethanolamine 0.5g

[0083] Flavor 0.5g

[0084] Appropriate amount of distilled water.

[0085] Thanks to the good ratio of active ingredients to gel matrix, the Codonopsis gel mask of the present invention has the following beneficial effects:

[0086] The present invention adopts CBSP-CP as a gel matrix and combines it with Codonopsis pilosula extract to prepare a Codonopsis pilosula gel mask, which is superior to ordinary Codonopsis pilosula masks, and has better stability, better conditioning and repairing effect, and stronger skin firming effect than gel masks prepared by combining different types of matrices such as traditional Carbopol 940, improved Carbopol ETD 2020, and polyacrylate cross-linked polymer-6 (Zen) with Codonopsis pilosula extract.

[0087] Stability. The Codonopsis gel mask prepared with CBSP-CP as the matrix and Codonopsis extract has better stability in appearance, viscosity and active index component content under high temperature (40℃, 60℃) and strong light (4500Lx) conditions.

[0088] Repair effect. The Codonopsis gel mask prepared with CBSP-CP as the matrix and Codonopsis extract can increase the proliferation activity of UVB-damaged HaCaT cells; reduce the LDH content in UVB-damaged HaCaT cells, and improve the antioxidant activity of UVB-damaged HaCaT cells (increase GSPH and reduce MDA). The Codonopsis gel mask with CBSP-CP as the matrix has better activity than the gel masks prepared with different types of matrices such as traditional Carbopol940, modified Carbopol ETD 2020, and polyacrylate cross-linked polymer-6 (Zen) and Codonopsis extract.

[0089] Moisturizing effect. The results of the skin water content measurement showed that the skin water content (MMV) of the gel mask with CBSP-CP as the matrix was significantly increased at each time point compared with the three commercially available matrices, indicating that the CBSP-CP composition of the present invention as a gel matrix combined with the Codonopsis pilosula extract helps to enhance the moisturizing effect.

[0090] Skin lightening effect. The Codonopsis gel mask prepared with CBSP-CP as the matrix and Codonopsis extract can significantly reduce the melanin content on the skin surface compared with three commercially available matrices, indicating that the Codonopsis gel mask with CBSP-CP as the matrix has better skin whitening and lightening effects.

[0091] Anti-wrinkle effect. The Codonopsis gel mask prepared with CBSP-CP as the matrix and Codonopsis extract can significantly reduce the depth and area of ​​wrinkles on the skin surface compared with three commercially available matrices, indicating that the Codonopsis gel mask with CBSP-CP as the matrix has a better whitening and lightening effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0092] Figure 1 The effects of CBSP-CP and a commercial matrix combined with Codonopsis pilosula extract gel mask on the skin appearance of aging mice;

[0093] Figure 2 The effects of CBSP-CP and the combination of commercial matrix and Codonopsis extract gel mask on skin wrinkles in aging mice;

[0094] Figure 3 The effects of CBSP-CP and commercial matrix combined with Codonopsis pilosula extract gel mask on the depth of wrinkles in aging mice;

[0095] Figure 4 The effects of CBSP-CP and commercial matrix combined with Codonopsis pilosula extract gel mask on the wrinkle area of ​​aging mice skin; Implementation

[0096] Bletilla striata polysaccharide Bletillastriata polysaccharide , BSP, Shaanxi Xi'an Panier Biotechnology Co., Ltd.); Carbomer (CP): Carbopol 940 (CP940, Lubrizol, USA), Carbopol 1342 (CP1342, Lubrizol, USA), Carbopol ETD 2020 (CP 2020, Lubrizol, USA), Carbopol Ultrez20 (CP U20, Lubrizol, USA) Polyacrylate Crosspolymer-6 (Zen, Seppic, France).

[0097] Example 1 Preparation of cationized Bletilla striata polysaccharide (CBSP)

[0098] BSP was dissolved in NaOH / urea solution to obtain a homogeneous system, and then NaOH was added to alkalize at room temperature, and the temperature was raised. NaOH was added to the system and the etherifying agent 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) was slowly added dropwise. The molar ratio of alkali to ether in the system was 1:1. The system was etherified in a constant temperature water bath for 5 h, and the pH was adjusted to neutral to end the etherification reaction. It was then placed in an activated dialysis bag with a molecular weight cutoff of 8-14 KD and purified with ultrapure water as the permeation medium. The exudate was replaced every 3 hours; after purification, it was filtered, rotary evaporated, freeze-dried, and crushed to obtain cationic Bletilla striata polysaccharide (CBSP).

[0099] Example 2 Preparation of a novel gel matrix material CBSP-CP (2:1)

[0100] 0.5% (w / v) CP 2020 was dispersed in 70°C deionized water, stirred at 400 rpm until uniform, and allowed to stand for 12 h to form a uniform, agglomerate-free sol, referred to as phase A;

[0101] 1.0% (w / v) CBSP was dispersed in deionized water at room temperature (25 °C) and stirred at 400 rpm until uniform to form a CBSP solution, referred to as phase B;

[0102] At room temperature (25°C), phase B was slowly added dropwise to phase A, and the pH was adjusted to 6.5 with saturated citric acid solution and triethanolamine. The mixture was freeze-dried to obtain a CBSP-CP composite matrix.

[0103] Example 3 Preparation of Codonopsis pilosula extract

[0104] The Codonopsis pilosula medicinal material was dried at 60°C for 2h and crushed into coarse powder. The coarse powder passed through a 10-mesh sieve was decocted three times with 10 times the amount of distilled water. The decoctions extracted three times were combined and filtered. The relative density of the filtrate was 1.30-1.40 when it was concentrated to 80°C. 500mL of the supernatant was stored at low temperature and away from light, which was the Codonopsis pilosula extract.

[0105] Example 4 Gel masks prepared by combining different gel bases with Codonopsis pilosula extract

[0106] Four kinds of Codonopsis gel masks were prepared under different matrix conditions using commercially available CP 940, CP 2020, Zen and CBSP-CP as matrix materials respectively. Other components and preparation methods were the same. The preparation performance quality of the Codonopsis gel mask prepared with CBSP-CP as the matrix was investigated.

[0107] Prescription composition:

[0108] Component name Weight ratio Codonopsis Extract Equivalent to 15g of medicinal material Gel Matrix 1g Sodium Hyaluronate 0.5g Vitamin C Ethyl Ether 1g glycerin 10ml 1,2-Propanediol 1g PEG-40 Hydrogenated Castor Oil 0.5g Phenoxyethanol 0.5g Menthol 1g D-Panthenol 1g Triethanolamine 0.5g essence 0.5g Distilled water Moderate

[0109] Take an appropriate amount of glycerin as a wetting agent, and fully swell four matrices of CP 940, CP 2020, Zen and CP-CBSP (Example 2) and a moisturizing agent, sodium hyaluronate, respectively, add an antioxidant and a Codonopsis pilosula extract, and stir until uniform; then add a penetration enhancer, a surfactant, a preservative, etc. and stir thoroughly, add deionized water to a certain amount, adjust the pH, and add an appropriate amount of flavor to prepare Codonopsis pilosula gel masks with different matrices.

[0110] Example 5 Gel facial mask prepared by combining different gel bases with Codonopsis pilosula extract

[0111] Four kinds of Codonopsis gel masks were prepared under different matrix conditions using commercially available CP 940, CP 2020, Zen and CBSP-CP as matrix materials respectively. Other components and preparation methods were the same. The preparation performance quality of the Codonopsis gel mask prepared with CBSP-CP as the matrix was investigated.

[0112] Prescription composition:

[0113] Component name Weight ratio Codonopsis Extract Equivalent to 30g of medicinal material Gel Matrix 1g Sodium Hyaluronate 0.5g Vitamin C Ethyl Ether 1g glycerin 10ml 1,2-Propanediol 1g PEG-40 Hydrogenated Castor Oil 0.5g Phenoxyethanol 0.5g Menthol 1g D-Panthenol 1g Triethanolamine 0.5g essence 0.5g Distilled water Moderate

[0114] Take an appropriate amount of glycerin as a wetting agent, and fully swell four matrices of CP 940, CP 2020, Zen and CP-CBSP (Example 2) and a moisturizing agent, sodium hyaluronate, respectively, add an antioxidant and a Codonopsis pilosula extract, and stir until uniform; then add a penetration enhancer, a surfactant, a preservative, etc. and stir thoroughly, add deionized water to a certain amount, adjust the pH, and add an appropriate amount of flavor to prepare Codonopsis pilosula gel masks with different matrices.

[0115] Example 6 Gel mask prepared by combining different gel bases with Codonopsis pilosula extract

[0116] Four kinds of Codonopsis gel masks were prepared under different matrix conditions using commercially available CP 940, CP 2020, Zen and CBSP-CP as matrix materials respectively. Other components and preparation methods were the same. The preparation performance quality of the Codonopsis gel mask prepared with CBSP-CP as the matrix was investigated.

[0117] Prescription composition:

[0118] Component name Weight ratio Codonopsis Extract Equivalent to 45g of medicinal material Gel Matrix 1g Sodium Hyaluronate 0.5g Vitamin C Ethyl Ether 1g glycerin 10ml 1,2-Propanediol 1g PEG-40 Hydrogenated Castor Oil 0.5g Phenoxyethanol 0.5g Menthol 1g D-Panthenol 1g Triethanolamine 0.5g essence 0.5g Distilled water Moderate

[0119] Take an appropriate amount of glycerin as a wetting agent, and fully swell four matrices of CP 940, CP 2020, Zen and CP-CBSP (Example 2) and a moisturizing agent, sodium hyaluronate, respectively, add an antioxidant and a Codonopsis pilosula extract, and stir until uniform; then add a penetration enhancer, a surfactant, a preservative, etc. and stir thoroughly, add deionized water to a certain amount, adjust the pH, and add an appropriate amount of flavor to prepare Codonopsis pilosula gel masks with different matrices.

[0120] Example 7 Study on the stability of Codonopsis gel mask prepared with CBSP-CP and commercially available matrix

[0121] The samples of Example 6 were all packaged in colorless transparent vials, and placed at 40°C, 60°C and strong light (4500lx) to investigate the changes in quality indicators such as appearance, viscosity and content of codonopsis pilosula gel masks prepared with different matrix materials. The results are shown in Tables 1-3, where "↓" and "↑" represent the degree of decrease or increase compared with day 0. The results show that the codonopsis pilosula gel mask prepared with CBSP-CP as the matrix has better stability in appearance, viscosity and content of active index components under high temperature and strong light conditions.

[0122] Table 1 Stability of gel masks prepared by combining different matrices with Codonopsis pilosula extract (high temperature 40℃) , n=3)

[0123]

[0124] Table 2 Stability of gel masks prepared by combining different matrices with Codonopsis pilosula extract (high temperature 60℃) , n=3)

[0125]

[0126] Table 3 Stability of gel masks prepared by combining different matrices with Codonopsis pilosula extract (strong light 4500lx) ( , n=3)

[0127]

[0128] Example 8 Investigation of Steady-State Viscosity

[0129] The steady-state apparent viscosity of the gel sample was measured by using a DV-Ⅲ rheometer, mode 1, SC4-34 # rotor, and shearing at a constant rate of 20s-1 for 120 s at room temperature 25°C. The steady-state viscosity results of the codonopsis gel mask prepared with different matrices are shown in Table 4. The codonopsis gel mask prepared with CBSP-CP (Example 6) has the smallest decrease in steady-state apparent viscosity, indicating that the viscosity of the codonopsis gel mask system prepared by the synergistic effect of CBSP-CP and codonopsis extract is best maintained.

[0130] Table 4 Steady-state viscosity results of Codonopsis pilosula gel mask prepared by combining different matrices with Codonopsis pilosula extract

[0131]

[0132] Example 9 Investigation of the repair effect

[0133] (1) Improve the proliferation activity of UVB-damaged HaCaT cells

[0134] Experimental methods

[0135] HaCaT cells in the logarithmic growth phase were taken, and the cell density was adjusted to 1×105 / mL with complete culture medium, and then inoculated into 96-well cell culture plates. The cells were cultured in a 37°C, 5% CO2 cell culture incubator until the cells in the 96-well plate grew to about 70%, and then randomly divided into a normal group (blank control group), a UVB irradiation group (model group), and a Codonopsis gel mask group prepared by combining CBSP-CP and commercially available matrix with Codonopsis extract, and pre-administered for 24 hours. During pre-administration, both the blank control group and the UVB irradiation group were given complete culture medium. The culture medium in the 96-well plate was discarded, and the plate was washed twice with PBS. 100 μL PBS was added to each well to cover it. The model group and the Codonopsis gel mask group prepared by combining various matrices with Codonopsis extract were irradiated with a UVB phototherapy device at a dose of 60 mJ / cm2. After irradiation, PBS was discarded, and complete culture medium was added. After incubation in an incubator at 37 ℃ and 5% CO2 for 24 h, the cell proliferation activity was detected by CCK8 method. The results are shown in Table 5.

[0136] Table 5 Effects of Codonopsis gel mask prepared by combining different matrices with Codonopsis extract on cell activity after UVB irradiation ( ± SD )(n=12)

[0137]

[0138] ### P< 0.001 vs blank control *** P< 0.001 vs Model * P< 0.05 vs Model

[0139] As shown in Table 5, the Codonopsis gel mask prepared by combining different matrices with Codonopsis extract can improve the proliferation activity of HaCaT cells damaged by UVB, among which the Codonopsis gel mask prepared by combining CBSP-CP as the matrix with Codonopsis extract has the best effect.

[0140] (2) Improving the antioxidant activity of UVB-damaged HaCaT cells - glutathione peroxidase

[0141] Experimental methods: HaCaT cells in the logarithmic growth phase were adjusted to a density of 1×105 / mL with complete culture medium and seeded into 60 mm culture dishes, and cultured in a cell culture incubator at 37 ºC and 5% CO2. After the cells in the culture dish grew to about 60%, they were randomly divided into a normal group (blank control group), a UVB irradiation group (model group), and a Codonopsis gel mask group prepared by combining CBSP-CP and commercially available matrix with Codonopsis extract, and pre-administered for 24 hours. During pre-administration, both the blank control group and the model group were given complete culture medium. The culture medium in the culture dish was discarded, and the culture dishes were washed twice with PBS. 100 μL PBS was added to each well to cover it. The model group and the Codonopsis gel mask group prepared by combining various matrices with Codonopsis extract were irradiated with a UVB phototherapy device at a dose of 60 mJ / cm2. After irradiation, PBS was discarded and complete culture medium was added. After incubation in an incubator at 37 ℃ and 5% CO2 for 24 h, the glutathione peroxidase (GSH-Px) level was detected using a kit. The results are shown in Table 6.

[0142] GSH-Px enzyme activity = × standard tube concentration (20µmol / L) × dilution factor ÷ reaction time ÷ (sample volume × sample protein content)

[0143] Press GSH-P X Assay Kit (Colorimetric Method) Instructions

[0144] Table 6 Effects of Codonopsis gel mask prepared by combining different matrices with Codonopsis extract on GSH-Px activity in HaCaT cells after UVB irradiation ( ± SD )(n=3)

[0145]

[0146] ## P<0.01 vs blank control * P < 0.05 vs model ** P < 0.01 vs model

[0147] As shown in Table 6, the Codonopsis gel mask prepared by combining different matrices with Codonopsis extract can significantly increase the GSH-PX activity of HaCaT cells damaged by UVB. Among them, the Codonopsis gel mask prepared by combining CBSP-CP as the matrix with Codonopsis extract has the best effect.

[0148] (3) Improving the antioxidant activity of UVB-damaged HaCaT cells - MDA

[0149] Experimental methods: HaCaT cells in logarithmic growth phase were adjusted to a density of 1×105 / mL with complete culture medium and seeded into 60 mm culture dishes, and cultured in a cell culture incubator at 37 ºC and 5% CO2. After the cells in the culture dish grew to about 60%, they were randomly divided into a normal group (blank control group), a UVB irradiation group (model group), and a Codonopsis gel mask group prepared by combining CBSP-CP and commercially available matrix with Codonopsis extract, and pre-administered for 24 hours. During pre-administration, both the blank control group and the model group were given complete culture medium. The culture medium in the culture dish was aspirated and discarded, and PBS was washed twice. 100 μL PBS was added to each well to cover it. The model group and the Codonopsis gel mask group prepared by combining each matrix with Codonopsis extract were irradiated with a UVB phototherapy device at a dose of 60mJ / cm2. After irradiation, PBS was discarded, and complete culture medium was added. After incubation in an incubator at 37 ℃ and 5% CO2 for 24 hours, the malondialdehyde (MDA) level was detected using a kit. The results are shown in Table 7.

[0150] Follow the instructions of the MDA assay kit (TBA method)

[0151] Table 7 Effects of different matrices combined with Codonopsis pilosula extract to prepare Codonopsis pilosula gel mask on MDA content in HaCaT cells after UVB irradiation ( ± SD )(n=3)

[0152]

[0153] ## P<0.01 vs blank control * P < 0.05 vs model

[0154] As shown in Table 7, the Codonopsis gel mask prepared by combining different matrices with Codonopsis extract can reduce the MDA content of HaCaT cells damaged by UVB, among which the Codonopsis gel mask prepared by combining CBSP-CP as the matrix with Codonopsis extract has the best effect.

[0155] Example 10 Investigation of moisturizing, skin lightening and anti-wrinkle effects

[0156] A skin aging model induced by ultraviolet (UV) radiation in ICR mice was established, and the effects of Codonopsis pilosula gel masks prepared by combining different matrices with Codonopsis pilosula extract on the skin moisture content, melanin and wrinkle formation of mice were investigated.

[0157] (1) Establishing an animal model of photoaging

[0158] First, use scissors and electric shavers to remove the hair on the back of the mice to fully expose their back skin. Then, use a homemade mouse fixer to fix the mice, and then irradiate the mice with the SS-03AB phototherapy device to create the model. Irradiate 5 days a week, rest 2 days, and irradiate for a total of 3 weeks. The total radiation dose UVB total dose: 9.45 J / cm 2 Total UVA dose: 94.5 J / cm 2 After the modeling was completed, the back skin of the mice with successful modeling was significantly thickened, the skin surface was rough, the skin was dry and hard, and obvious wrinkles were visible.

[0159] (2) Experimental groups

[0160] The mice were randomly divided into 6 groups, namely: a blank control group, 5 mice, skin preparation, device fixation, no irradiation; a model group, 5 mice, skin preparation, device fixation, irradiation; a compound gel group (a Codonopsis gel mask prepared by combining different matrices with Codonopsis extract in Example 6) group, 5 mice, skin preparation, device fixation, local administration of Codonopsis gel mask prepared with corresponding matrix on the back skin, and irradiation.

[0161] (3) Effect of Codonopsis gel mask on skin moisture in UV-induced photoaging mice

[0162] The probe of the skin moisture content tester (Cornemeter CM 825, Courage and Khazaka, Germany) is based on the principle of capacitance for measurement. The dielectric constant of water is 81, and the dielectric constant of other substances is usually less than 7. Water is the substance with the largest dielectric constant on the skin. When the moisture content changes, the capacitance value of the skin also changes. Therefore, the moisture content of the skin surface can be analyzed by measuring the skin capacitance value. The measured value is a relative value. The unit is expressed in CU (Corneometer Units).

[0163] In the experiment, the changes in skin moisture content after using different Codonopsis gel masks were tested. Specifically: the blank control group was not irradiated, and the model group and different Codonopsis gel mask groups were all irradiated with ultraviolet rays. The irradiation conditions were as shown in the establishment of the photoaging animal model. Irradiation was performed 5 days a week, and the skin moisture content was measured on the 6th day (i.e. 6d, 12d, 18d) after 5 days of irradiation every week. The skin moisture content tester was used to measure the skin MMV value of the test area, and each test area was tested 3 times to take the average value. The change in the skin moisture content MMV value is reflected in the test period, and the moisture content of the experimental area changes over time. The larger the value, the greater the moisture content, and vice versa, the smaller the moisture content. The measurement results are shown in Table 8.

[0164] Table 8 Effects of Codonopsis gel mask prepared by combining different matrices with Codonopsis extract on skin moisture of photoaged mice induced by UV irradiation ( ± SD )(n=5)

[0165]

[0166] ### P<0.001 vs blank control group ## P<0.01 vs blank control group** P<0.01 vs model group* P<0.05 vs model group

[0167] As can be seen from Table 8, during the test period, the MMV values ​​of skin moisture in the test area of ​​the Codonopsis gel mask group were higher than those of the model group, and the Codonopsis gel mask prepared by combining CBSP-CP with Codonopsis extract had the greatest effect on increasing the moisture content of photoaged skin and improving the symptoms of dryness of photoaged skin.

[0168] (4) Effect of Codonopsis Gel Mask on Melanin Content in Photoaged Mice

[0169] The tissue viability imager (Tissue Viability Imager TiVi 700, WheelsBridge AB, Sweden) is based on the linear polarized light emitted by the white light illuminator and passed through the polarization filter to reach the skin surface to be analyzed. Most of the linear polarized light is directly reflected by the skin surface and blocked by the detector filter. Only a part of the polarized light that passes through the lower layer of the skin and is absorbed by the red blood cells passes through the filter and enters the detector. In the polarized light reaching the detector, the green light component is weakened due to the absorption of red blood cells in the blood vessels, and the red component is almost unchanged. The absorption of green and red light by other tissues around the red blood cells is basically the same. In this way, the blood vessel microcirculation status in the deep layer of the skin can be obtained, and the red blood cell concentration value at each point in the image, namely the TiVi value, can be obtained. The images were taken with TiVi 700, and the melanin content was analyzed using TiVi97 pigment analysis software.

[0170] In the experiment, the changes in skin melanin content after using the Codonopsis gel mask were tested. Specifically: the blank control group was not irradiated, and the model group and the different Codonopsis gel mask groups were all irradiated with ultraviolet rays. The irradiation conditions were as described in the establishment of the photoaging animal model. The irradiation was performed 5 days a week, and the skin melanin content was measured on the 6th day (i.e. 6d, 12d, 18d) after the 5th day of irradiation every week. The measurement results are shown in Table 9.

[0171] Table 9 Effects of Codonopsis gel mask prepared by combining different matrices with Codonopsis extract on the melanin content of UV-induced photoaging mice skin ( ± SD )(n=5)

[0172]

[0173] ### P<0.001 vs blank control group ## P<0.01 vs blank control group** P<0.01 vs model group* P<0.05 vs model group

[0174] As can be seen from Table 9, during the test period, the melanin content in the test area of ​​the Codonopsis gel mask group was lower than that of the model group, and the Codonopsis gel mask prepared by combining CBSP-CP with Codonopsis extract had the best effect on reducing the melanin content of photoaged skin and improving the pigmentation problem of photoaged skin.

[0175] (5) Effect of Codonopsis Gel Mask on Skin Wrinkles in Photoaged Mice

[0176] At the third week, images were taken using a tissue viability imager (Tissue Viability Imager TiVi 700, WheelsBridge AB, Sweden) (4), and wrinkle analysis software Wrinkle Analyzer TiVi90 calculated the depth and volume of wrinkles by analyzing wrinkle contours. The depth and volume of wrinkles in mice with UV radiation-induced skin aging were shown in Figure 2. Figure 2 , 3 , 4. The test results showed that the wrinkle depth and volume of the test area of ​​the codonopsis gel mask group were lower than those of the model group, and the codonopsis gel mask prepared by combining CBSP-CP with codonopsis extract had the best effect on reducing wrinkles.

[0177] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art who is familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A Codonopsis gel mask, comprising: Gel matrix, Codonopsis pilosula extract, wherein the gel matrix is ​​made of cationized Bletilla striata polysaccharide and carbomer in a weight ratio of 2:1, wherein the cationized Bletilla striata polysaccharide BSP is prepared by the following method: dissolving Bletilla striata polysaccharide BSP in NaOH / urea solution to obtain a homogeneous system, then adding NaOH to alkalize at room temperature, heating, adding NaOH to the system and slowly dripping etherifying agent 3-chloro-2-hydroxypropyltrimethylammonium chloride CTA, the molar ratio of alkali to ether in the system is 1:1, etherifying in a constant temperature water bath for 5 hours, adjusting the pH to neutral, and the etherification reaction is completed; then placing it in an activated dialysis bag with a molecular weight cutoff of 8-14 KD, purifying it with ultrapure water as the permeation medium, and replacing the exudate every 3 hours; after purification, suction filtration, rotary evaporation, freeze drying, and crushing to obtain the cationized Bletilla striata polysaccharide CBSP.

2. the Codonopsis pilosula gel facial mask according to claim 1, further comprising a pH adjusting agent.

3. The Codonopsis gel mask according to claim 2 further comprises an additive, characterized in that: The additive is selected from one or more of moisturizers, preservatives, transdermal absorption enhancers, antioxidants, and flavors.

4. The Codonopsis pilosula gel mask according to any one of claims 1 to 3, characterized in that: The gel mask is composed of the following ingredients: 。 5. The Codonopsis pilosula gel mask according to any one of claims 1 to 3, characterized in that: The weight ratio of each component is: 。 6. The Codonopsis pilosula gel mask according to any one of claims 1 to 3, characterized in that: The weight ratio of each component is: 。 7. The Codonopsis pilosula gel mask according to any one of claims 1 to 3, characterized in that: The weight ratio of each component is: 。

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