Compositions for controlling unwanted organisms and methods for controlling same

Abstract

The present application belongs to the field of biotechnology, and particularly relates to a combination for controlling non-desired organisms and a method for controlling non-desired organisms. The present application controls non-desired organisms, particularly harmful organisms, by sequentially applying (A) a compound and / or a drug having a non-desired organism promoting effect and (B) a compound and / or a drug having a non-desired organism inhibiting effect. The present application provides a combination for controlling non-desired organisms and a method for controlling non-desired organisms, which has a good inhibiting effect on non-desired organisms and has a certain application prospect.

Description

Technical Field

[0001] This invention belongs to the field of biotechnology, specifically relating to a composition and method for controlling unintended organisms. Background Technology

[0002] Biological disasters occur frequently in my country's agricultural and forestry production and protection processes, and the control of many important pests still relies on the use of pesticides. Due to this long-term dependence on pesticides in agricultural and forestry production and protection, many agricultural pests, harmful plants, and harmful microorganisms have developed resistance. This resistance has led to a continuous increase in pesticide use, which not only seriously affects the control of pests but also damages the environment, threatens human health, and shortens the lifespan of most pesticide varieties, even leading to their obsolescence. For example, in some areas, various pests such as cotton bollworm, western flower thrips, and cotton spider mite have developed resistance to pyrethroid insecticides (Chen Chengyu). In some areas, beet armyworm has also developed resistance to a variety of pesticides, including organochlorine, organophosphate, carbamate, pyrethroid, abamectin, indoxacarb, chlorantraniliprole, spinosad, tebufenozide, acetamiprid, and chlorpyrifos (Yang Yihua). The irrational use of these pesticides has led to a rapid increase in the resistance of pests, which has an adverse impact on the subsequent control of agricultural and forestry pests.

[0003] Agricultural and forestry pests refer to a general term for animals that adversely affect agricultural and forestry production and the protection of plants and animals. These include nematodes, arachnids, and insects. Nematodes, belonging to the phylum Nematoda, are incredibly diverse, with over 28,000 recorded species and many more yet to be named. Nematodes can be classified into three types based on their lifestyle: free-living, animal-parasitic, and plant-parasitic. Some plant-parasitic nematodes cause significant economic losses to agriculture and forestry. The most common genera affecting agriculture include root-knot nematodes (Meloidogyne), cyst nematodes (Heterodera), root-rot nematodes (Pratylenchus), stem nematodes (Ditylenchus), xiphinema, long-needle nematodes (Longidorus), and trichiodorus; while the pine wood nematode (Bursaphelenchus xylophilus) has severely impacted pine forests in my country. Pine wilt nematode is a common parasitic nematode of pine trees, transmitted by vector insects such as the pine sawyer beetle, leading to pine wilt disease. The disease was first discovered in my country in 1982 at the Sun Yat-sen Mausoleum in Nanjing. Subsequently, several disease centers formed in Shandong, Anhui, Guangdong, and Zhejiang provinces, spreading outwards and causing widespread outbreaks and devastation in these provinces, resulting in the death of large numbers of pine trees. To date, pine wilt disease has spread to 666 counties (cities and districts) in 18 provinces across the country, affecting 16.719 million mu (approximately 1.1 million hectares) in 2019, resulting in the death of 19.467 million pine trees. It has directly threatened the survival of 900 million mu (approximately 66.7 million hectares) of pine forests in my country, causing immense damage to my country's forestry production and ecological security. As of now, the cumulative number of pine trees lost due to pine wilt disease nationwide still reaches billions of trees, resulting in direct economic losses and ecological service value losses exceeding hundreds of billions of yuan.

[0004] Currently, nematode control primarily relies on chemical control methods, employing highly effective chemical insecticides. The active ingredients mainly include abamectin, thiamethoxam, methyl isofenphos, ethoprophos, methyl bromide, aldicarb, amino oligosaccharides, dazomet, fenitrothion, carbofuran, and thiocarbofuran, among others. Most of these products are organophosphates, highly toxic, and their effectiveness against pine wood nematodes parasitizing woody plants like pines is generally limited. For example, abamectin at recommended concentrations has a mortality rate of less than 30% against pine wood nematodes. Furthermore, nematodes easily develop resistance to these broad-spectrum synthetic pesticides, and many of these insecticides are highly toxic, extremely toxic, or have high residue levels, causing severe pollution to humans, the environment, microorganisms, and water resources. Therefore, the application of chemical insecticides is significantly limited.

[0005] Arachnida is a class of arachnids within the phylum Arthropoda, with approximately 50,000 known species worldwide, making it the largest class within the subphylum Chelicerata. Common arachnids include spiders, scorpions, ticks, and mites. Arachnida are closely related to the environment and humans, but some species have a detrimental impact on agricultural production. For example, mites such as *Eotetranychus smithi*, *Petrobia Latens*, *Eriophyes disoar*, *Tetranychus cinnabarinus*, *T. viennensis*, and *Aculops niphocladae* have all seriously affected agricultural production in my country.

[0006] Insects, belonging to the class Insecta of the phylum Arthropoda, are a diverse and numerous group of animals, found in almost every corner of the world. As of the beginning of the 21st century, over one million insect species were known to humankind, with many more yet to be discovered. Insects are generally classified into the orders Orthoptera, Collembola, Thysanura, Coleoptera, and Hemiptera, with the most common including butterflies, aphids, flies, mosquitoes, cockroaches, and locusts. Insects have a significant impact on the environment and on human health; in agricultural production, their impact is primarily manifested in their destructive effects. For example, rice planthoppers, powdery mildew, corn borers, cotton bollworms, wheat rust, cotton aphids, rice sheath blight, rice blast, wheat aphids, wheat red spider mites, and locusts have all become major pests and diseases seriously affecting agricultural production in my country.

[0007] Currently, locust control primarily employs biological and chemical methods to manage the occurrence and spread of locust infestations. Biological control mainly utilizes biological agents, controlling locust damage by protecting and utilizing natural enemies or releasing artificially bred parasitic predators. While environmentally friendly, biological control suffers from drawbacks such as altering locust community structure, high costs, and the lack of mature control methods. Chemical control typically uses organophosphates, pyrethroids, and their compound pesticides. While effective in rapidly controlling locust outbreaks and spread, the large-scale use of chemical pesticides can lead to poisoning in humans and livestock, environmental pollution, and the killing of other organisms, posing safety risks. Furthermore, continuous use of the same pesticides over many years results in increasing locust resistance, leading to declining control effectiveness. To avoid antagonistic effects from single-drug use, new pesticides need to be continuously screened and replaced. Despite these serious challenges, pesticide application has made significant contributions to pest and disease control and ensuring food production. In the foreseeable future, chemical control will remain a key technology for addressing agricultural biological disasters. Therefore, it is necessary to find insecticidal compounds and / or pesticides with higher safety, or to improve the efficacy of insecticidal compounds and / or pesticides.

[0008] Harmful agricultural and forestry plants mainly include weeds and invasive plants, such as barnyard grass, lambsquarters, amaranth, wild oats, crabgrass, foxtail grass, reeds, caltrop, water sedge, purslane, purple-stemmed eupatorium, Spartina alterniflora, alligator weed, water hyacinth, ragweed, poisonous wheat, airplane grass, Mikania micrantha, golden bell vine, false sorghum, wedelia triloba, five-clawed golden dragon, Italian cocklebur, and spiny nightshade.

[0009] Hormones are a class of chemical substances with highly efficient information transmission functions, playing a vital regulatory role in the body's metabolism, growth, development, and reproduction. In agriculture and forestry, plant hormones, insect hormones, and microbial growth regulators are commonly used.

[0010] Plant hormones, also known as natural plant hormones or endogenous plant hormones, refer to trace amounts of organic compounds produced within plants that regulate (promote or inhibit) their own physiological processes. Six classes of hormones are known to be produced in plants: auxins, gibberellins, cytokinins, abscisic acid, ethylene, and brassinosteroids. These are all simple small-molecule organic compounds, but their physiological effects are extremely complex and diverse, ranging from influencing cell division, elongation, and differentiation to affecting germination, rooting, flowering, fruiting, sex determination, dormancy, and abscission. Therefore, plant hormones play a crucial regulatory role in plant growth and development. Currently, various hormone-based herbicides have been developed, which promote plant growth at low concentrations but can kill plants at high concentrations. Examples include 2,4-D butyl ester, dicamba, and quinclorac.

[0011] Insect hormones can be chemically classified into three categories: ① proteins, including peptides such as brain hormones, diapause hormones, and lipokinins; ② terpenes, such as juvenile hormones; and ③ sterols, such as ecdysone. Ecdysone, also known as 20-hydroxyecdysone (20E), is a precursor of 20-hydroxyecdysone (E) synthesized by the prothoracic gland during insect metamorphosis, regulated by prothoracicotropic hormone (PTTH). After release and transformation, it participates in the developmental processes of molting and metamorphosis. Within a certain concentration range, ecdysone can promote the development and metabolism of insects and nematodes, but excessively high concentrations can kill the insects. Currently, various ecdysone-based insecticidal compounds have been developed. Their insecticidal mechanism mimics the ecdysone's entry into the insect's body to initiate molting. These insecticides continuously induce the molting response, ultimately causing the insect to die because it cannot form a structurally complete new epidermis.

[0012] Meanwhile, existing technologies involve combining ecdysone-based insecticides with insecticidal compounds for pest control. For example, in CN201410412011.0, an insecticidal composition is prepared by mixing an insecticidal compound with an insect growth regulator, and then using it as an insecticidal composition. However, this existing technology has the following characteristics: firstly, the active ingredient that plays the main insecticidal role in this invention is a new compound obtained in its own preparation; secondly, the ecdysone receptor agonist compound used in this invention also has a toxic effect on pests; and thirdly, this invention does not demonstrate the insecticidal effect of the above-mentioned compound combined with the ecdysone receptor agonist.

[0013] To address the aforementioned problems, this invention is proposed. Summary of the Invention

[0014] The purpose of this invention is to address the problems existing in the prior art by providing a composition and method for controlling unintended organisms.

[0015] To achieve the above objectives, the present invention adopts the following technical solution:

[0016] A composition for controlling unintended organisms, the composition comprising component A and component B, wherein the mass ratio of composition A to composition B is (10... -4 ~1):(10 -2 ~1), Composition A and Composition B are used in sequence; wherein: component A is a compound and / or drug that promotes the action of unwanted organisms, and component B is a compound and / or drug that inhibits the action of unwanted organisms.

[0017] A compositional formulation for controlling unintended biological agents, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~ 10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0018] A method for controlling unintentional organisms includes the following steps:

[0019] Component A and component B of the composition are applied to unintended organisms in a sequential manner, with a time interval of 6 to 144 hours between the application of component A and component B.

[0020] Alternatively, the formulation containing component A and the formulation containing component B are applied sequentially to an unintended organism, with an interval of 6–144 hours between the applications of the formulation containing component A and the formulation containing component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~ 10 4 ppm.

[0021] In the above text, the application targets of component A include, but are not limited to: the unintentional organism itself, the environment in which the unintentional organism exists, and the unintentional organism's water source or food; any method that can enter the unintentional organism's body and be utilized by it to achieve the effect of promoting the unintentional organism's growth is acceptable. The application methods of component A include, but are not limited to: all methods of using the active ingredient of component A as described in the prior art.

[0022] In the above text, the application targets of component B include, but are not limited to: the undesired organism itself, the environment in which the undesired organism exists, and the water source or food of the undesired organism; any method that can enter the body of the undesired organism and be utilized by it to achieve the effect of promoting the growth of the undesired organism is acceptable. The application methods of component B include, but are not limited to: all methods of using the active ingredient of component B as described in the prior art.

[0023] The "promoting effect" mentioned above refers to enhancing the physiological activities of unintended organisms through certain means, including promoting the metabolism of unintended organisms, promoting the growth, development and reproduction of the organism, influencing its aging process, enhancing the organism's adaptation to the environment, and breaking dormancy.

[0024] The "inhibitory effect" mentioned above refers to the reduction in the number and / or activity of unintended biological populations through certain means, including causing unintended organisms to deviate from the normal developmental process in terms of growth and reproduction, to cause internal functional disorders, to enter dormancy, to experience a decline in function, and to die.

[0025] The unwanted organisms mentioned in this invention refer to organisms that are not desired, specifically, under certain conditions, humans desire to control their population size, including harmful organisms.

[0026] The harmful organisms described in this invention refer to organisms that, under certain conditions, pose a threat to human life, production, and even survival, including harmful animals, harmful plants, and harmful microorganisms. In agriculture and forestry, they mainly include agricultural and forestry pests, harmful plants, and harmful microorganisms.

[0027] The agricultural and forestry pests described in this invention refer to pests that, under certain conditions, harm human life, production, and even survival, including pests belonging to the class Insecta, class Arachnida, and phylum Nematoda.

[0028] The composition for controlling pests consists of component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~1):(10 -2 ~1); wherein: component A is a compound and / or drug that promotes the growth of harmful organisms, and component B is a compound and / or drug that inhibits the growth of harmful organisms.

[0029] A compositional formulation for controlling pests, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~ 10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0030] A method for controlling pests includes the following steps:

[0031] Component A and component B of the composition are applied to the pests in a sequential manner, with a time interval of 6 to 144 hours between the application of component A and component B.

[0032] Alternatively, the formulation containing component A and the formulation containing component B are applied to the pest in sequence, with an interval of 6–144 hours between the applications; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm.

[0033] In the above text, the application targets of component A and preparations containing component A include, but are not limited to: the pest itself, the environment in which the pest exists, the pest's water source, or its food; any method that can enter the pest's body and be utilized by it to promote the pest's growth is acceptable. The application methods of component A include, but are not limited to, all methods of using the active ingredient of component A as described in the prior art.

[0034] In the above text, the application targets of component B and preparations containing component B include, but are not limited to: the pest itself, the environment in which the pest exists, the pest's water source, or its food; any method that can enter the pest's body and be utilized by it to promote the pest's growth is acceptable. The application methods of component B include, but are not limited to, all methods of using the active ingredient of component B as described in the prior art.

[0035] The "promoting effect" mentioned above refers to enhancing the physiological activities of harmful organisms through certain means, including promoting the metabolism of harmful organisms, promoting the growth, development and reproduction of the organism, affecting its aging process, enhancing the organism's adaptation to the environment, and breaking dormancy.

[0036] The "inhibitory effect" mentioned above refers to the reduction in the population and / or activity of harmful organisms through certain means, including causing the growth and reproduction of harmful organisms to deviate from the normal development process, causing internal functional disorders, entering dormancy, declining function, and death.

[0037] The composition for controlling pests consists of component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~ 1):(10 -2 ~1); wherein: component A is a compound and / or drug that promotes the action of pests, and component B is a compound and / or drug that inhibits the action of pests.

[0038] A compositional formulation for controlling pests, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0039] A method for controlling pests includes the following steps:

[0040] Components A and B of the composition are applied to the pests in a sequential order, with a time interval of 6 to 144 hours between the application of components A and B.

[0041] Alternatively, the formulation containing component A and the formulation containing component B are applied to the pests sequentially, with an interval of 6–144 hours between application; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm.

[0042] In the above text, the application targets of component A and preparations containing component A include, but are not limited to: the pest itself, the environment in which the pest lives, the pest's water source, or its food; any method that can enter the pest's body and be utilized by the pest to promote its growth is acceptable. The application methods of component A include, but are not limited to: all methods of using the active ingredient of component A as described in the prior art.

[0043] In the above text, the application targets of component B and preparations containing component B include, but are not limited to: the pest itself, the environment in which the pest lives, the pest's water source, or its food; any method that can enter the pest's body and be utilized by the pest to promote its growth is acceptable. The application methods of component B include, but are not limited to: all methods of using the active ingredient of component B as described in the prior art.

[0044] The compound and / or drug that promotes the growth of pests may be: ecdysone and its analogues and / or antijuvenile hormone and its analogues.

[0045] The molting hormone and its analogues described in this invention refer to a class of natural or synthetic compounds that can interfere with the normal growth and development of insects and cause pests to molt prematurely.

[0046] The ecdysone described in this invention may specifically be: α-ecdysone, β-ecdysone, 20-hydroxyecdysone, achyranthesone, podophyllinone, basilanone, etc.

[0047] The ecdysone analogues described in this invention may specifically be: cyclotetracycline, chlorfenapyr, methoxyfenozide and / or tebufenozide, etc.

[0048] The anti-juvenile hormone and its analogues described in this invention refer to a class of compounds that can interfere with the normal growth and development of pests and resist the effects of juvenile hormones. Among them, juvenile hormone compounds can inhibit the appearance of adult characteristics during the larval stage, allowing the larvae to remain in the larval state after molting; during the adult stage, they have the functions of controlling development, generating sex attraction, and promoting egg maturation.

[0049] Antijuvenile hormones and their analogues may specifically include: kojic acid and its derivatives, rosin acid and its derivatives, fluoromethylvaleric acid, ethyl-4,2-terpinene-carboxy-oxygen, ethyl-3-methyl-laurate, piperidine, compactin, serotonin, SM-1, and YA. 20 And SD-III, allyl compounds, imidazole compounds (such as KK-42, KK-22, SSP-11), etc.

[0050] The compound and / or drug that promotes the growth of pests is preferably a ecdysone compound, more preferably α-ecdysone, β-ecdysone, 20-hydroxyecdysone and / or oxendone.

[0051] The compounds and / or drugs that have inhibitory effects on pests according to the present invention include: compounds and / or pesticides that have inhibitory effects on insect pests, compounds and / or pesticides that have inhibitory effects on arachnid pests, and compounds and / or pesticides that have inhibitory effects on nematode pests.

[0052] The "promoting effect" mentioned above refers to enhancing the physiological activities of pests through certain means, including promoting the metabolism of pests, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0053] The "inhibitory effect" mentioned above refers to the reduction of pest population size and / or activity through certain means, including causing pest growth and reproduction to deviate from the normal development process, internal functional disorders, dormancy, decreased function, and death.

[0054] A composition for controlling insect pests, comprising component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes the activity of insect pests, and component B is a compound and / or drug that inhibits the activity of insect pests; the components A and B are used in sequence.

[0055] A compositional formulation for controlling insect pests, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~ 10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0056] A method for controlling insect pests includes the following steps:

[0057] Component A and component B of the composition are applied to insect pests in a sequential manner, with a time interval of 6 to 144 hours between the application of component A and component B.

[0058] Alternatively, the formulation containing component A and the formulation containing component B are applied sequentially to insect pests, with an interval of 6–144 hours between application; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~ 10 4 ppm.

[0059] Furthermore, the concentration of the active ingredient in component A is preferably 10. -1 ~10 2 ppm; the concentration of the active ingredient in component B is preferably 10 ppm. -2 ~10 3 ppm.

[0060] In the above text, the application targets of component A and preparations containing component A include, but are not limited to: the insect pests themselves, the environment in which the insect pests live, and the water or food sources of the insect pests; any method that can enter the insect pest's body and be utilized by it to promote the growth of the insect pest is acceptable. The application methods of component A include, but are not limited to, all methods of using the active ingredient of component A as described in the prior art.

[0061] In the above text, the application targets of component B and preparations containing component B include, but are not limited to: the insect pests themselves, the environment in which the insect pests live, and the water or food sources of the insect pests; any method that can enter the insect pest's body and be utilized by it to promote the growth of the insect pest is acceptable. The application methods of component B include, but are not limited to, all methods of using the active ingredient of component B as described in the prior art.

[0062] The "promoting effect" mentioned above refers to enhancing the physiological activities of insect pests through certain means, including promoting the metabolism of insect pests, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0063] The "inhibitory effect" mentioned above refers to the reduction in the population size and / or activity of insect pests through certain means, including causing insect pests to deviate from the normal developmental process in terms of growth and reproduction, internal functional disorders, dormancy, decreased function, and death.

[0064] A composition for controlling arachnid pests, comprising component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes the activity of arachnid pests, and component B is a compound and / or drug that inhibits the activity of arachnid pests; the concentration of component A is 10 -1 ~ 10 3 ppm, the concentration of component B is 10 -2 ~10 4 ppm; Component A and Component B are used in sequence.

[0065] A compositional formulation for controlling arachnid pests, wherein the formulation is prepared separately from component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~ 10 3 ppm, the concentration of the active ingredient in a formulation containing components is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0066] A method for controlling arachnid pests includes the following steps:

[0067] Components A and B of the composition are applied to arachnid pests in a sequential order, with a time interval of 6 to 144 hours between the application of components A and B.

[0068] Alternatively, the formulation containing component A and the formulation containing component B are applied to arachnid pests sequentially, with an interval of 6–144 hours between application; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~ 10 4 ppm.

[0069] The "promoting effect" mentioned above refers to enhancing the physiological activities of arachnid pests through certain means, including promoting the metabolism of arachnid pests, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0070] The "inhibitory effect" mentioned above refers to the reduction in the population size and / or activity of arachnid pests through certain means, including causing arachnid pests to deviate from the normal developmental process in terms of growth and reproduction, internal functional disorders, dormancy, decreased function, and death.

[0071] The combination for controlling nematode pests consists of component A and component B, with a mass ratio of component A to component B of (10:1). -4 ~1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes the activity of nematode pests, and component B is a compound and / or drug that inhibits the activity of nematode pests; the components A and B are used in sequence.

[0072] A compositional formulation for controlling nematode pests, comprising formulations prepared separately from component A and component B. The formulation containing component A contains at least one or more compounds of component A as its active ingredient, and the formulation containing component B contains at least one or more compounds of component B as its active ingredient. The concentration of the active ingredient in the formulation containing component A is 10... -1 ~ 10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0073] A method for controlling nematode pests includes the following steps:

[0074] Components A and B of the composition are applied to nematode pests in a sequential manner, with a time interval of 6 to 144 hours between the application of components A and B.

[0075] Alternatively, the formulation containing component A and the formulation containing component B are applied sequentially to nematode pests, with an interval of 6–144 hours between application; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~ 10 4 ppm.

[0076] Furthermore, the concentration of the active ingredient in component A is preferably 10. -1 ~10 2 ppm; the concentration of the active ingredient in component B is preferably 10 ppm. -2 ~10 3 ppm.

[0077] In the above text, the application targets of component A and preparations containing component A include, but are not limited to: the nematodes themselves, the environment in which the nematodes live, and the nematodes' water sources or food sources; any method that can enter the nematode's body and be utilized by it to promote nematode growth is acceptable. The application methods of component A include, but are not limited to: all methods of using the active ingredient of component A as described in the prior art.

[0078] In the above text, the application targets of component B and preparations containing component B include, but are not limited to: the nematodes themselves, the environment in which the nematodes live, and the nematodes' water sources or food sources; any method that can enter the nematode's body and be utilized by it to promote nematode growth is acceptable. The application methods of component B include, but are not limited to: all methods of using the active ingredient of component B as described in the prior art.

[0079] The "promoting effect" mentioned above refers to enhancing the physiological activities of nematode pests through certain means, including promoting the metabolism of nematode pests, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0080] The "inhibitory effect" mentioned above refers to the reduction in the population size and / or activity of nematode pests through certain means, including causing nematode pests to deviate from the normal developmental process, disrupt their internal functions, enter dormancy, experience decreased function, or die.

[0081] The compounds and / or drugs that promote the growth of insect, arachnid, and / or nematode pests may be: ecdysone and its analogues and / or antijuvenile hormone and its analogues.

[0082] The molting hormone and its analogues described in this invention refer to a class of natural or synthetic compounds that can interfere with the normal growth and development of pests and cause them to molt prematurely.

[0083] The ecdysone described in this invention may specifically be: α-ecdysone, β-ecdysone, 20-hydroxyecdysone, achyranthesone, podophyllinone, basilanone, etc.

[0084] The ecdysone analogues described in this invention may specifically be: cyclotetracycline, chlorfenapyr, methoxyfenozide and / or tebufenozide, etc.

[0085] The anti-juvenile hormone and its analogues described in this invention refer to a class of compounds that can interfere with the normal growth and development of pests and resist the effects of juvenile hormones. Among them, juvenile hormone compounds can inhibit the appearance of adult characteristics during the larval stage, allowing the larvae to remain in the larval state after molting; during the adult stage, they have the functions of controlling development, generating sex attraction, and promoting egg maturation.

[0086] Antijuvenile hormones and their analogues may specifically include: kojic acid and its derivatives, rosin acid and its derivatives, fluoromethylvaleric acid, ethyl-4,2-terpinene-carboxy-oxygen, ethyl-3-methyl-laurate, piperidine, compactin, serotonin, SM-1, and YA. 20 And SD-III, allyl compounds, imidazole compounds (such as KK-42, KK-22, SSP-11), etc.

[0087] The compound and / or drug that promotes the growth of pests is preferably a ecdysone compound, more preferably α-ecdysone, β-ecdysone, 20-hydroxyecdysone and / or oxendone.

[0088] The compounds and / or pesticides that have inhibitory effects on insect pests include, but are not limited to:

[0089] Organothiophosphates: acephate, azamethiphos, azinphos-methyl, chlorpyrifos, chlorpyrifos-methyl, chlorfenvinphos, dizinon, dichlorvos, didrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isothion Isoxathion, malathion, methamidophos, methidathion, methyl-parathion, mevinphos, monocrotophos, oxydemeton-methyl, paraoxon, parathion, phenthoate, phosalone, phosmet, phosphamidon, phorate, phoxim, pirimiphos-methyl, profenofos, prothiofos, sulprophos, tetrachlorvinphos, terbufos, triazophos, trichlorfon;

[0090] Carbamates: alanycarb, aldicarb Bendicarb, benfuracarb, carbaryl, carbofuran, carbosulfan, fenoxycarb, furathiocarb, methiocarb, methomyl, oxamyl, pirimicarb, propoxur, thiodicarb, triazamate;

[0091] Synthetic pyrethrins: allethrin, bifenthrin, cyfluthrin, (RS) cyhalothrin, cyphenothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, and cypermethrin. (fenvalerate), imiprothrin, lambda-cyhalothrin, permethrin, prallethrin, pyrethrin I and II, resmethrin, silafluofen, tau-fluvalinate, tefluthrin, tetramethrin, tralomethrin, transfluthrin, profluthrin, dimefluthrin;

[0092] Nicotine receptor agonists / antagonists: clothianidin, dinotefuran, imidacloprid, thiamethoxam, nitenpyram, acetamiprid, thiacloprid, 1-(2-chlorothiazol-5-ylmethyl)-2-nitrimino-3,5-dimethyl-[1,3,5]triazinane;

[0093] GABA antagonists: endosulfan, ethiprole, fipronil, vaniliprole, pyrafluprole, pyriprole, N-5-amino-1-(2,6-dichloro-4-methylphenyl)-4-sulfinamoyl-1H-pyrazole-3-thiocarboxamide;

[0094] Macrolides: abamectin, emamectin, milkemectin, lepimectin, spinosad, spintoram.

[0095] Mitochondrial electron transport chain inhibitors (METI) I acaricides: fenazaquin, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim;

[0096] METIII substances: acequinocyl, fluacyprim, and hydramethylnone;

[0097] Decoction agent: chlorfenapyr;

[0098] Oxidative phosphorylation inhibitors: cyhexatin, diafenthiuron, fenbutatin oxide, propargite;

[0099] Mixed-function oxidase inhibitor: piperonyl butoxide;

[0100] Sodium channel blockers: Indoxacarb, metaflumizone;

[0101] Others include: benclothiaz, bifenazate, cartap, flonicamid, pyridalyl, pymetrozin, thiocyclam, flubendiamide, chlorantraniliprole, cyazypyr (HGW86); cyenopyrafen, flupyrazofos, cyflumetofen, amidoflumet, imicyafos, bistrifluron, pyrifluquinazone, azadirachtin, matrine, etc.

[0102] The compounds and / or pesticides that have inhibitory effects on arachnid pests include, but are not limited to: abamectin, acequinocyl, flufenoxuron, aldicarb, α-cypermethrin, amiditin, amiton, amitraz, aramite, arsenic trioxide, ethyl glutathione, methyl glutathione, azobenzene, azocyclotin, azophos, benomyl, benzoximate, benzyl benzoate, bifenazate, bifenthrin, binapacryl, and bromocycline. en), bromothion, ethyl bromothion, bromopropylate, methyl ethyl ketone carbide, camphechlor, carbanolate, carnallium, carbophenothion, carvacrol, chinomethionat, chlorbenside, chlordimeform, brofenol, chlorfensulphide, chlorfenapyr. chlorfenvinphos, chlorobenzilate, chloromebuform, chloromethiuron, chloropropylate, chlorthiophos, clofentezine, closantel, coumaphos, crotamiton, crotoxyphos, cycloprate, cyenopyrafren, and dicofol. Cyflumetofen, Trifluralin, Tricyclic Tin, Cypermethrin, Flymethrin, DDT, Demerol, Methyl Demerol, Demerol-O, Demerol-O-Methyl, Demerol-S, Demerol-S-Methyl, Diethylurea, Dialifos, Diazinon, Dichlofluanid, Dicofluol, Dieldrin, Dienochlor, Diflovidazin, Dimethoate, Dinex, Dinobuton, Difenoconazole(dinocap), dinocton, dinopenton, dinosulfon, dinoterbon, dioxathion, diphenyl sulfone, ethion, DNOC, endothion, ethion, ethoate-methyl, etoxazole, fenazaflor, fenazaquin, fenbutatin Oxide), Fenothiocarb, Cypermethrin, Fenpyroximate, Fenson, Fentrifanil, Cypermethrin, Fipronil, Fluacrypyrim, Fluazuron, Flubenzimine, Flucycloxuron, Fluenetil, Flufenoxuron, Flubenside, Lambda-cyhalothrin, Formosanitizer, Formosanitizer, Genit, Halfenprox, Heptenphos, Hexachlorophene Hexythiazox, isocarbophos, lindane, malathion, phosmet, methicrifos, methamidophos, imidacloprid, chlorpyrifos, mipafox, phosmet, dibromophos, nifluridide, omethoate, chlorpyrifos, oxydeprofos, oxydisulfoton, parathion, permethrin, fenthion, phorate, fenthion, phosmet, phosmet, phosmet, phosmet, phorate, methyl pyrimidin, propargite, acetamiprid, prothidathion, prothoate, pyridaben, pyrimidifen, quinalphosQuinalphos, quintiofos, schradan, sophamide, spirodiclofen, sulfluramid, sulfotep, sulfur, tau-flufenoxuron, tebufenpyrad, TEPP, carbendazim, tetrasul, thiocarboxime, thiofanox, methyl methamidophos, thioquinox, thuringiensin, triarathene, triazophos, trichlorfon, and / or vamidothion, etc.

[0103] The compounds and / or pesticides that have inhibitory effects on nematode pests include, but are not limited to, 1,3-dichloropropene, benclothiaz, dazomet, dazomet-sodium, dibromochloropropane (DBCP), dichloroisopropyl ether (DCIP), diamidoafos, fluensulfone, fosthiazate, furfural, neonicotinoids, isamidoafos, isazofos, isazofos, metam, metam-ammonium, metam-potassium, metam-sodium, phosphocarb, thionazin, azadirachtin, matrine, ethylparaben, and / or dimethyl azelaate, etc.

[0104] The composition for controlling locusts consists of component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~ 1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes locust growth, and component B is a compound and / or drug that inhibits locust growth; the components A and B are used in sequence.

[0105] A compositional formulation for controlling locusts, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0106] A method for controlling locusts includes the following steps:

[0107] Component A and component B of the composition are applied to locusts in a sequential manner, with the time interval between application of component A and component B being 6 to 144 hours, preferably 24 to 96 hours, and more preferably 24 to 72 hours.

[0108] Alternatively, the formulation containing component A and the formulation containing component B are applied to locusts sequentially, with the time interval between application of the formulation containing component A and the formulation containing component B being 6–144 hours, preferably 24–96 hours, and more preferably 24–72 hours; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm.

[0109] Furthermore, the concentration of the active ingredient in component A is preferably 10. -1 ~10 2 ppm, more preferably 10 -1 ~10ppm, more preferably 10 -1 ~5ppm; the preferred concentration of active ingredient in component B is 10. -2 ~10 3 ppm, more preferably 0.05 to 600 ppm.

[0110] In the above text, the application targets of component A and preparations containing component A include, but are not limited to: the locust itself, the environment in which the locust lives, the locust's water source or food; any method that can enter the locust's body and be utilized by the locust to promote locust growth is acceptable. The application methods of component A include, but are not limited to: all methods of using the active ingredient of component A as described in the prior art.

[0111] In the above text, the application targets of component B and preparations containing component B include, but are not limited to: the locust itself, the environment in which the locust lives, the locust's water source or food; any method that can enter the body of the unintentional locust and be utilized by it to achieve the effect of inhibiting the unintentional locust is acceptable. The application methods of component B include, but are not limited to: all methods of using the active ingredient of component B as described in the prior art.

[0112] The compounds and / or drugs that promote locust growth may be: ecdysone and its analogues and / or antijuvenile hormone and its analogues.

[0113] The molting hormone and its analogues described in this invention refer to a class of natural or synthetic compounds that can interfere with the normal growth and development of insects and cause them to molt prematurely.

[0114] The ecdysone described in this invention may specifically be: α-ecdysone, β-ecdysone, 20-hydroxyecdysone, achyranthesone, podophyllinone, berberine, etc. Preferably, it is 20-hydroxyecdysone.

[0115] The ecdysone analogues described in this invention may specifically be: cyclotetracycline, chlorfenapyr, methoxyfenozide and / or tebufenozide, etc.

[0116] The anti-juvenile hormone and its analogues described in this invention refer to a class of compounds that can interfere with the normal growth and development of insects and resist the effects of juvenile hormones. Among them, juvenile hormone compounds can inhibit the appearance of adult characteristics during the larval stage, allowing the larvae to remain in the larval state after molting; during the adult stage, they have the functions of controlling development, generating sex attraction, and promoting egg maturation.

[0117] Antijuvenile hormones and their analogues may specifically include: kojic acid and its derivatives, rosin acid and its derivatives, fluoromethylvaleric acid, ethyl-4,2-terpinene-carboxy-oxygen, ethyl-3-methyl-laurate, piperidine, compactin, serotonin, SM-1, and YA. 20 And SD-III, allyl compounds, imidazole compounds (such as KK-42, KK-22, SSP-11), etc.

[0118] The compound and / or drug that promotes locust growth is preferably a ecdysone compound, more preferably α-ecdysone, β-ecdysone, 20-hydroxyecdysone and / or oxendone.

[0119] The compounds and / or pesticides that have an inhibitory effect on locusts:

[0120] Organothiophosphates: acephate, azamethiphos, azinphos-methyl, chlorpyrifos, chlorpyrifos-methyl, chlorfenvinphos, dizinon, dichlorvos, didrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isothion Isoxathion, malathion, methamidophos, methidathion, methyl-parathion, mevinphos, monocrotophos, oxydemeton-methyl, paraoxon, parathion, phenthoate, phosalone, phosmet, phosphamidon, phorate, phoxim, pirimiphos-methyl, profenofos, prothiofos, sulprophos, tetrachlorvinphos, terbufos, triazophos, trichlorfon, dichlorvos;

[0121] Carbamates: alanycarb, aldicarb Bendicarb, benfuracarb, carbaryl, carbofuran, carbosulfan, fenoxycarb, furathiocarb, methiocarb, methomyl, oxamyl, pirimicarb, propoxur, thiodicarb, triazamate;

[0122] Synthetic pyrethrins: allethrin, bifenthrin, cyfluthrin, (RS) cyhalothrin, cyphenothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, and cypermethrin. (fenvalerate), imiprothrin, lambda-cyhalothrin, permethrin, prallethrin, pyrethrin I and II, resmethrin, silafluofen, tau-fluvalinate, tefluthrin, tetramethrin, tralomethrin, transfluthrin, profluthrin, dimefluthrin;

[0123] Nicotine receptor agonists / antagonists: clothianidin, dinotefuran, imidacloprid, thiamethoxam, nitenpyram, acetamiprid, thiacloprid, 1-(2-chlorothiazol-5-ylmethyl)-2-nitrimino-3,5-dimethyl-[1,3,5]triazinane;

[0124] GABA antagonists: endosulfan, ethiprole, fipronil, vaniliprole, pyrafluprole, pyriprole, N-5-amino-1-(2,6-dichloro-4-methylphenyl)-4-sulfinamoyl-1H-pyrazole-3-thiocarboxamide;

[0125] Macrolides: abamectin, emamectin, milkemectin, lepimectin, spinosad, spintoram.

[0126] Mitochondrial electron transport chain inhibitors (METI) I acaricides: fenazaquin, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim;

[0127] METIII substances: acequinocyl, fluacyprim, and hydramethylnone;

[0128] Decoction agent: chlorfenapyr;

[0129] Oxidative phosphorylation inhibitors: cyhexatin, diafenthiuron, fenbutatin oxide, propargite;

[0130] Mixed-function oxidase inhibitor: piperonyl butoxide;

[0131] Sodium channel blockers: Indoxacarb, metaflumizone;

[0132] Others: benclothiaz, bifenazate, cartap, flonicamid, pyridalyl, pymetrozin, sulfur, thiocyclam, flubendiamide, chlorantraniliprole, cyazypyr (HGW86); cyenopyrafen, flupyrazofos, cyflumetofen, amidoflumet, imicyafos, bistrifluron, pyrifluquinazone, azadirachtin, matrine.

[0133] The preferred compounds and / or pesticides that inhibit locusts are: imidacloprid, malathion, deltamethrin, azadirachtin, matrine, beta-cypermethrin, emamectin, and / or triazophos.

[0134] The composition for controlling locusts comprises component A and component B, wherein component A is ecdysone and component B is azadirachtin; the components A and B are used in a sequential order.

[0135] A compositional formulation for controlling locusts, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0136] A method for controlling locusts includes the following steps:

[0137] Component A and component B of the composition are applied to locusts in a sequential order, with a time interval of 6 to 144 hours between the application of component A and component B.

[0138] Alternatively, the formulation containing component A and the formulation containing component B are applied to locusts sequentially, with an interval of 6–144 hours between application; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm.

[0139] Furthermore, the time interval between the administration of component A or a formulation containing component A and component B or a formulation containing component B is 24–48 h and 48–72 h.

[0140] In the above text, component A or preparations containing component A are applied to the locusts' water sources or food sources; component B or preparations containing component B are applied to the locusts themselves or their surrounding environment. Any method that allows the locusts to utilize component B or preparations containing component B to achieve the effect of inhibiting locusts is acceptable.

[0141] The locusts mentioned above refer to all insects within the order Orthoptera of the class Insecta.

[0142] The "promoting effect" described in this invention refers to enhancing the physiological activities of locusts through certain means, including promoting locust metabolism, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0143] The "inhibitory effect" described in this invention refers to reducing the number and / or activity of locust populations through certain means, including causing locusts to deviate from the normal developmental process in terms of growth and reproduction, causing internal functional disorders, entering dormancy, declining function, and death.

[0144] The composition for controlling nematodes consists of component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~ 1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes the action of nematodes; component A and component B are used in sequence.

[0145] A compositional formulation for controlling nematodes, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0146] A method for controlling nematodes includes the following steps:

[0147] Components A and B of the composition are applied to nematodes in a sequential manner, with the time interval between application of components A and B being 6 to 144 hours, preferably 10 to 72 hours, and more preferably 10 to 48 hours.

[0148] Alternatively, the formulation containing component A and the formulation containing component B are applied to nematodes sequentially, with the time interval between application of the formulation containing component A and the formulation containing component B being 6–144 h, preferably 10–72 h, and more preferably 10–48 h; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm.

[0149] Furthermore, the concentration of the active ingredient in component A is preferably 10. -1 ~10 2 ppm, more preferably 10 -1 ~10ppm, more preferably 10 -1 ~5ppm; the preferred concentration of active ingredient in component B is 10. -2 ~10 3 ppm, more preferably 10 -1 ~10 3 ppm, more preferably 10 -1 ~600ppm.

[0150] The compound and / or drug that promotes nematode growth regulation may be: ecdysone and its analogues and / or antijuvenile hormone and its analogues.

[0151] The molting hormone and its analogues described in this invention refer to a class of natural or synthetic compounds that can interfere with the normal growth and development of insects and cause them to molt prematurely.

[0152] The ecdysone described in this invention may specifically be: α-ecdysone, β-ecdysone, 20-hydroxyecdysone, achyranthesone, podophyllinone, berberine, etc. Preferably, it is 20-hydroxyecdysone.

[0153] The ecdysone analogues described in this invention may specifically be: cyclotetracycline, chlorfenapyr, methoxyfenozide and / or tebufenozide, etc.

[0154] The anti-juvenile hormone and its analogues described in this invention refer to a class of compounds that can interfere with the normal growth and development of insects and resist the effects of juvenile hormones. Among them, juvenile hormone compounds can inhibit the appearance of adult characteristics during the larval stage, allowing the larvae to remain in the larval state after molting; during the adult stage, they have the functions of controlling development, generating sex attraction, and promoting egg maturation.

[0155] Antijuvenile hormones and their analogues may specifically include: kojic acid and its derivatives, rosin acid and its derivatives, fluoromethylvaleric acid, ethyl-4,2-terpinene-carboxy-oxygen, ethyl-3-methyl-laurate, piperidine, compactin, serotonin, SM-1, and YA. 20 And SD-III, allyl compounds, imidazole compounds (such as KK-42, KK-22, SSP-11), etc.

[0156] The compound and / or drug that promotes nematode growth is preferably a ecdysone compound, more preferably α-ecdysone, β-ecdysone, 20-hydroxyecdysone and / or oxendone.

[0157] The compounds and / or pesticides that have inhibitory effects on nematodes include, but are not limited to, 1,3-dichloropropene, benclothiaz, dazomet, dazomet-sodium, dibromochloropropane (DBCP), dichloroisopropyl ether (DCIP), diamidoafos, fluensulfone, fosthiazate, furfural, neonicotinoids (imicyafos), isamidoafos, isazofos, metam, and metam-ammonium. Metam-potassium, metam-sodium, phosphocarb, thionazin, emamectin, carbofuran, iprodione, isofenphos-methyl, phoxim, cartap, ethoprophos, azadirachtin, matrine, ethyl parabens and / or dimethyl azelaate, etc.

[0158] The compounds and / or pesticides that have an inhibitory effect on nematodes are preferably emamectin, carbofuran, iprodione, isofenphos-methyl, phoxim, cartap, ethoprophos, azadirachtin, matrine, ethylparaben, and / or dimethyl azelaate, etc.

[0159] The composition for controlling nematodes consists of component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~ 1):(10 -2 ~1), wherein: component A is ecdysone, and component B is ethylparaben and / or dimethyl azelaate; components A and B are used in sequence.

[0160] A compositional formulation for controlling nematodes, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 The concentration of active ingredient in the formulation containing component B is 0.1 to 600 ppm, and the formulations containing component A and component B are used in sequence.

[0161] A method for controlling nematodes includes the following steps:

[0162] Components A and B of the composition are applied to nematodes in a sequential manner, with the time interval between application of components A and B being 6 to 144 hours, preferably 10 to 72 hours, and more preferably 10 to 48 hours.

[0163] Alternatively, the formulation containing component A and the formulation containing component B are applied to nematodes sequentially, with the time interval between application of the formulation containing component A and the formulation containing component B being 6–144 h, preferably 10–72 h, and more preferably 10–48 h; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~10ppm, and the concentration of the active ingredient in formulations containing component B is 0.1~600ppm.

[0164] In the above text, the time interval between the application of component A and component B can be further set to: 10–24 h, 24–48 h.

[0165] In the above text, component A is applied to the nematodes themselves or the environment infected by the nematodes. Any method in which component A can be used by the nematodes to achieve the effect of inhibiting the nematodes is acceptable. Component B is applied to the nematodes themselves or the environment in which they live. Any method in which component B can be used by the nematodes to achieve the effect of inhibiting the nematodes is acceptable.

[0166] The nematodes mentioned refer to all animals harmful to agriculture and forestry, including those belonging to the genera *Scalyx*, *Root-knot Nematodes*, *Cyclocarya*, and / or *Stem Nematodes*.

[0167] The "promoting effect" described in this invention refers to enhancing the physiological activities of nematodes through certain means, including promoting nematode metabolism, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0168] The "inhibitory effect" described in this invention refers to the reduction in the population size and / or activity of nematodes through certain means, including causing nematode growth and reproduction to deviate from the normal developmental process, internal functional disorders, dormancy, decreased function, and death.

[0169] A composition for controlling harmful plants, comprising component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes the growth of harmful plants, and component B is a compound and / or drug that inhibits the growth of harmful plants; the components A and B are used in sequence.

[0170] A compositional formulation for controlling harmful plants, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~ 10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0171] A method for controlling harmful plants includes the following steps:

[0172] Components A and B of the composition are applied to the harmful plants in a sequential order, with a time interval of 6 to 144 hours between the application of components A and B.

[0173] Alternatively, the formulation containing component A and the formulation containing component B are applied to the harmful plants sequentially, with an interval of 6–144 hours between the applications; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm.

[0174] The "promoting effect" described in this invention refers to enhancing the physiological activities of harmful plants through certain means, including promoting the metabolism of harmful plants, promoting their growth, development and reproduction, influencing their aging process, enhancing their adaptation to the environment, and breaking dormancy.

[0175] The "inhibitory effect" described in this invention refers to reducing the number and / or activity of harmful plant populations through certain means, including causing harmful plants to deviate from the normal development process in terms of growth and reproduction, to cause internal functional disorders, to enter dormancy, to experience a decline in function, and to die.

[0176] A composition for controlling harmful microorganisms, comprising component A and component B, wherein the mass ratio of component A to component B is (10:1). -4 ~1):(10 -2 ~1), wherein: component A is a compound and / or drug that promotes the activity of harmful microorganisms, and component B is a compound and / or drug that inhibits the activity of harmful microorganisms; the components A and B are used in sequence.

[0177] A compositional formulation for controlling harmful microorganisms, wherein the formulation is prepared by separately preparing component A and component B, wherein the active ingredient in the formulation containing component A contains at least one or more compounds of component A, and the active ingredient in the formulation containing component B contains at least one or more compounds of component B; the concentration of the active ingredient in the formulation containing component A is 10. -1 ~ 10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~10 4 ppm; the formulations of component A and component B are used in sequence.

[0178] A method for controlling harmful microorganisms includes the following steps:

[0179] Component A and component B of the composition are applied to harmful microorganisms in a sequential manner, with a time interval of 6 to 144 hours between the application of component A and component B.

[0180] Alternatively, the formulation containing component A and the formulation containing component B are applied sequentially to harmful microorganisms, with an interval of 6–144 hours between the applications; the concentration of the active ingredient in the formulation containing component A is 10%. -1 ~10 3 ppm, the concentration of the active ingredient in the formulation containing component B is 10 -2 ~ 10 4 ppm.

[0181] The "promoting effect" described in this invention refers to enhancing the physiological activities of harmful microorganisms through certain means, including promoting the metabolism of harmful microorganisms, promoting their growth, development and reproduction, influencing their aging process, enhancing the body's adaptation to the environment, and breaking dormancy.

[0182] The "inhibitory effect" described in this invention refers to the reduction of the number and / or activity of harmful microbial populations through certain means, including causing harmful microorganisms to deviate from the normal development process, cause internal dysfunction, enter dormancy, decline in function, or die.

[0183] In this invention, components A and B can be prepared into solutions, emulsions, suspensions, powders, pastes, granules and / or gels, or any other type known in the art, by any means known in the art.

[0184] In this invention, components A and B may include other conventional adjuvants, the selection of which depends on the specific application or pesticide. These adjuvants may be solvents, solid carriers, surfactants (e.g., surfactants, other solubilizers, protective colloids, wetting agents, and / or binders), organic and inorganic thickeners, fungicides, antifreeze agents, defoamers, colorants, and / or adhesives.

[0185] The solvent may be one or more of water and organic solvents; the organic solvent may be mineral oil fractions such as kerosene, diesel oil, coal tar, vegetable or animal oils, aliphatic, cyclic and aromatic hydrocarbons, alcohols, glycols, ketones, etc.

[0186] The solid carrier may be mineral soil such as silica, silica gel, silicates, talc, kaolin, limestone, lime, chalk, red basalt, loess, clay, dolomite, diatomite, calcium sulfate, magnesium sulfate, magnesium oxide, ground synthetic materials, fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, and plant-derived products such as grain flour, bark flour, wood flour, nut shell powder, cellulose powder, etc.

[0187] The surfactants may be nonionic surfactants such as polyethylene glycol higher fatty acid esters, polyoxyethylene alkyl ethers, polyoxyethylene alkyl aryl ethers, polyoxyethylene aryl phenyl ethers, sorbitan monoalkyl esters, ethynyl alcohol and ethynyl glycol, and their epoxy alkyl adducts; anionic surfactants such as alkyl aryl sulfonates, dialkyl sulfonates, lignin sulfonates, naphthalene sulfonates and their condensates, alkyl sulfate salts, alkyl phosphate salts, alkyl aryl sulfate salts, alkyl aryl phosphate salts, polyoxyethylene alkyl ether sulfate salts, polyoxyethylene alkyl aryl ether sulfate salts, polyoxyethylene aryl phenyl ether sulfate salts, and polycarboxylic acid type polymer surfactants; cationic surfactants such as tetraalkylammonium salts, alkylamines, and alkylpyrimidine salts; amphoteric surfactants such as alkyl betaine, alkyl amine oxides, alkyl imidazoline betaine, and amino acids; and organosilicon surfactants and fluorinated surfactants.

[0188] The adhesive may be starch, alginic acid, glycerin, polyvinylpyrrolidone, polyurethane, polyethylene glycol, polypropylene glycol, polybutene, polyvinyl alcohol, gum arabic, liquid paraffin, ethyl cellulose, carboxymethyl cellulose, xanthan gum and / or polyvinyl acetate.

[0189] The combinations of the present invention exist in the form of agricultural chemical compositions. These chemical compositions can be any type known in the art, such as solutions, emulsions, suspensions, powders, pastes, granules, and / or gels. The chemical compositions are prepared in any manner known in the art.

[0190] The beneficial effects of this invention are as follows:

[0191] Despite the serious challenges facing chemical control, it remains a key technology for addressing agricultural biological disasters in the foreseeable future. Therefore, it is essential to find insecticidal compounds and / or pesticides with higher safety profiles, or to enhance the effectiveness of these compounds and / or pesticides.

[0192] The present invention provides a composition for controlling unintended biological activity, which creatively combines a compound or analogue that promotes the activity of unintended biological activity with a compound or / or drug that inhibits the activity of unintended biological activity to form a new composition for combined use. By first applying the compound or analogue that promotes the activity, and then applying the compound or / or drug that inhibits the activity of unintended biological activity after 6 to 144 hours, the inhibitory effect on unintended biological activity is enhanced.

[0193] Specifically, regarding the composition for controlling pests (locusts, nematodes), this invention creatively combines ecdysone with compounds and / or drugs that have inhibitory effects on pests (locusts, nematodes) to form a new composition for combined use. By first applying the ecdysone compound, and then applying the compounds and / or drugs that have inhibitory effects on pests (locusts, nematodes) 6–144 hours later, the toxic effect on pests (locusts, nematodes) is enhanced. Attached Figure Description

[0194] Figure 1 This is a diagram showing the effect of molting hormone on the developmental stages of a 3-instar East Asian migratory locust in Example 1.

[0195] Figure 2 The graph shows the mortality rate of the East Asian migratory locust caused by the ecdysone-enhanced azadirachtin in Example 1.

[0196] Figure 3 The graph shows the mortality rate of pine wood nematodes caused by dimethyl azelaate, which enhances the ecdysone effect, in Example 2.

[0197] Figure 4 The graph shows the mortality rate of pine wood nematodes caused by ecdysone-enhanced ethylparaben in Example 2. Specific Implementation

[0198] The technical solutions in the embodiments of the present invention will be clearly and completely described below. The described embodiments are only a part of the present invention, and not the entirety of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0199] The insect class includes Microcoryphia (order Sclerotia), Thysanura (order Lepidoptera), Ephemeroptera (order Ephemeroptera), Odonata (order Odonata), Plecoptera (order Plecoptera), Blattaria (order Blattaria), Mantodea (order Mantis), Grylloblattodea (order Grylloblattodea), and others. Order Mantophasmatodea, Phasmatodea, Embioptera, Orthoptera, Dermaptera, Order Zoraptera, Anoplura, Psocoptera, Thysanoptera, Hemiptera, Neuroptera, Megaloptera, Raphidioptera, Coleoptera Co All animals including leoptera, Diptera, Siphonaptera, Strepsiptera, Mecoptera, Trichoptera, Lepidoptera, and Hymenoptera.

[0200] Insects of the order Blattodea, such as: Periplaneta fuliginosa, Periplaneta americana, Periplaneta australasiae, Blattella germanica, Blatta orientalis, etc.

[0201] The insects of the order Orthoptera include, for example: European mole cricket Gryllotalpagryllotalpa, East Asian migratory locust Locustamigratoria, double-banded black locust Melanoplus bivittatus, red-legged black locust M. femurrubrum, blood locust M. sanguinipes, Rocky Mountain locust M. spretus, red-winged locust Nomadacris septemfasciata, South American desert locust Schistocerca americana, desert locust S. gregaria, and house cricket Acheta domesticus, etc.

[0202] The insects of the order Thysanoptera include, for example: Frankliniella fusca, F. occidentalis, F. tritici, Haplothrips tritici, Heliothiripshaemorrhoidalis, Scirtothrips citri, Thrips oryzae, T. palmmi, and Ts tabaci.

[0203] The insects of the order Hemiptera include, for example: *Acrosternum hilare*, *Blissus leucopterus*, *Dysdercus cingulatus*, *Eurygaster integriceps*, *Lygus hesperus*, *L. lineolaris*, *L. pratensis*, *Nezara viridula*, *Acyrthosiphon pisum*, *Adelges laricis*, *Aonidiella aurantii*, *Aphis fabae*, *A. gossypii*, *A. pomi*, *Aulacorthum solani*, *Bemisia tabaci*, *Brevicoryne brassicae*, and *Dalbulus*. maidis, Dreyfusia piceae, Empoasca fabae, Laodelphax striatella, Megoura viciae, Metopolophium dirhodum, Myzus persicae, M. cerasi, Nephotettix cincticeps, Nilaparvata lugens, Perkinsiella saccharicida, Phorodonhumuli, Psylla mali, P. pyri, P. pyricola, Rhopalosiphum maidis, Schizaphis graminum, Sitobion avenae, Sogatella furcifera, Toxoptera citricida, Trialeurodes abutilonea, greenhouse whitefly T. vaporariorum, and grape phylloxera Viteus vitifoliae.

[0204] The insects of the order Diptera, such as: Mexican fruit fly Anastrepha ludens, Mediterranean fruit fly Ceratitis capitata, sorghum gall fly Contarinia sorghicola, melon fruit fly Dacus cucurbitae, olive fruit fly D. oleae, rapeseed leaf gall fly Dasineura brassicae, wheat seed fly Delia coarctata, cabbage seed fly D. radicum, leaf miner Hydrellia griseola, Hyleniyia platura, American serpentine leafminer Liriomyzasativae, trifoliate leafminer L. trifolii, Hessian gall fly Mayetiola destructor, Asian rice gall fly Orseoliaoryzae, Swedish wheat stem fly Oscinella frit, beet leaf miner Pegomya hyoscyami, Phorbia antiqua, P. brassicae, P. coarctata, cherry fruit fly Rhagoletis The following species are listed: *Aedes aegypti* and *Rhagoletis pomonella*, as well as *Aedes aegypti*, *Aedes vexans*, *Aedes albopictus*, *Anopheles maculipennis*, *Chrysomya bezziana*, *Cochliomyia hominivorax*, *Chrysamya macellaria*, *Cordylobia anthropophaga*, *Culexpipiens*, *Fannia canicularis*, *Gasterophilus intestinalis*, *Glossina morsitans*, *Haematobia irritans*, *Haplodiplosis equestris*, *Hypoderma lineata*, *Lucilia cuprina*, *L. sericata*, *Muscadomestica*, *Muscina stabulans*, and *Oestrus*. ovis, horsefly Tabanus bovinus, and black fly Simulium damnosum.

[0205] The following are examples of Coleoptera insects: *Anthonomus grandis*, *A. pomorum*, *Apion vorax*, *Atomaria linearis*, *Blastophagus piniperda*, *Cassidanebulosa*, *Cerotoma trifurcata*, *Chaetocnema tibialis*, *Conoderus vespertinus*, *Criocerisasparagi*, *Cryptolestes ferrugineus*, *Dendroctonus rufipennis*, *Diabrotica longicornis*, *Diabrotica virgifera*, *Epilachna varivestis*, *Epitrix hirtipennis*, and *Eutinobothrus*. *Brahsiliensis*, *Hylobius abietis*, *Hyperapostica*, *Ips typographus*, *Lema bilineata*, *Lema melanopus*, *Leptinotarsadecemlineata*, *Limonius californicus*, *Lissorhoptrus oryzophilus*, *Meligethes aeneus*, *Oulema oryzae*, *Otiorhynchus sulcatus*, *Otiorhynchus ovatus*, *Phaedoncochleariae*, *Phyllopertha horticola*, and *Phyllophaga* sp.The species include: *Phyllotreta striolata*, *Popillia japonica*, *Psylliodes napi*, *Scolytus intricatus* and *Sitonalineatus*, as well as *Bruchus rufimanus*, *Bruchus pisorum*, *Sitophilus granarius*, *Lasioderma serricorne*, *Oryzaephilus surinamensis*, *Rhyzopertha dominica*, *Sitophilus oryzae*, *Tribolium castaneum*, *Trogoderma granarium*, and *Zabrotes subfasciatus*.

[0206] The Lepidoptera insects mentioned include: *Adoxophyes orana* (cotton brown leaf roller), *Agrotis ipsilon* (small cutworm), *Agrotis segetum* (yellow cutworm), *Alabama argillacea* (cotton leaf roller), *Anticarsia gemmatalis* (soybean leaf roller), *Argyresthia conjugella* (soybean leaf roller), *Autographa gamma* (gamma leaf roller), *Cacoecla murinana* (gamma leaf roller), *Capua reticulana* (tobacco leaf roller), *Choristoneura fumiferana* (spruce leaf roller), *Chilo partellus* (grass moth), *Choristoneura occidentalis* (western spruce leaf roller), *Cirphis unipuncta* (spotted armyworm), *Cnaphalocrocis medinalis* (rice leaf roller), *Crocidolomi abinotalis* (African hairy moth), *Cydiapomonella* (codling moth), *Dendrolimus pini* (European pine caterpillar), *Diaphania nitidalis* (cucumber silkworm moth), and *Diatraea* (southern corn borer). * *Earias grandiosella*, *Earias insulana*, *Elasmopalpus lignosellus*, *Eupoecilia ambiguella*, *Feltia subterranea*, *Grapholitha funebrana*, *Grapholithamolesta*, *Heliocoverpazea*, *Hellula undalis*, *Hibernia defoliaria*, *Hypliantria cunea*, *Hyponomeuta malinellus*, *Keiferia lycopersicella*, *Lambdina fiscellaria*, *Laphygmaexigua*, *Leucopterascitella*, *Lobesia botrana*, *Loxostegesticticalis*, *Lymantria dispar*, *Lymantria* monacha, peach leafminer Lyonetia clerkella, tobacco hawk moth Manduca sexta, tent caterpillar Malocosomaneustria, cabbage cutworm Mamestrabrassicae, Mocisrepanda, Operaphthera brumata, Oryyia pseudotsugata, Ostrinia nubilalis, Pandemisheparana, Panolis flamnea, Pectinophora gossypiella, Phthorimaea operculella, Phyllocnistis citrella, Pieris brassicae, Plathapena scabra, Platynota stultana, Plutella xylostella, Prays citri, Prays oleae, Pseudoplusia includens, Rhyacionia frustran, Scropipalpula absoluta, Sesamia The following species are listed: inferens, Sparganothispilleriana, Spodoptera frugiperda, Spodoptera littoralis, Spodoptera litura, Tortrix viridana, Trichoplusia ni, Tryporyza incertulas, Galleria mellonella, Sitotrogacerealella, Ephestia cautella, and Tineola bisselliella.

[0207] The following are examples of Hymenoptera insects: turnip leaf bee *Athalia rosae*, large-headed leafcutter ant *Attacephalotes*, *Atta sexdens*, *Atta texana*, *Hoplocampaminuta*, *Hoplocampa testudinea*, Argentine rainbow ant *Iridomyrmex humilis*, purple rainbow ant *Iridomyrmex purpureus*, small yellow house ant *Monomorium pharaonis*, tropical fire ant *Solenopsis geminata*, red fire ant *Solenopsis invicta*, black fire ant *Solenopsis richteri*, and white-legged cunning ant *Technomyrmex albipes*.

[0208] The class Arachnida includes all animals in the following orders: Scorpiones, Pseudoscorpionida, Opiliones, Acarina, Palpigradi, Uropigi, Schizomida, Amblypygi, Araneae, Ricinulei, and Solifugae.

[0209] The order Acari includes pests such as: alfalfa bryobia praetiosa, fruit bryobia rubrioculus, chicken skin mite Dermanyssus gallinae, hornbeam leaf mite Eotetranychus carpini, Lewisite leaf mite Eotetranichus lewisi, Eutetranychus banksia, oriental leaf mite Eutetranychus orientalis, citrus gall mite Eriophyes sheldoni, grape gall mite Eriophyes vitis, redfoot mite Halotydeus destructor, meadow small claw mite Oligonychus pratensis, coffee small claw mite Oligonychus coffeae, apple claw mite Panonychus ulmi, and citrus claw mite Panonychus citri.Blue oat mite, citrus rust mite (Phyllocoptruta oleivora), lateral polyphagotarsonemus latus, sheep itch mite (Psoroptes ovis), scabies mite (Sarcoptes scabiei), white tarsonemus (Tarsonemus pallidus), cinnabarinus (Tetranychus cinnabarinus), kanzawai (Tetranychus kanzawai), pacificus (Tetranychus pacificus), urticae (Tetranychus urticae), red tarsonemus (Tetranychus), carinatus (Calacarus carinatus), polyphagotarsonemus latus, American tick (Amblyomma americanum), variegatum (Amblyomma variegatum), Argaspersicus (Argaspersicus), annulatus (Boophilus annulatus), decoloratus (Boophilus decoloratus), and boophilus microta (Boophilus microta). The ticks include microplus, Dermacentorsilvarum, Hyalomma truncatum, Ixodes ricinus, Ixodes rubicundus, Ornithodorus moubata, Otobius megnini, Rhipicephalus apendiculatus, Rhipicephalusevertsi, and Rhipicephalus microplus.

[0210] The locusts described in this invention refer to all insects within the order Orthoptera, class Insecta.

[0211] The order Orthoptera includes all animals, including the superfamily Tettigonioidea, Gryllacridoidea, Grylloidea, Gryllotapoidea, Gryllotalpoidea, Tetrigoidea, Eustacoidea, and Acridoidea.

[0212] The superfamily Jatropha includes all animals, including Batrachididae, Tripetaloceridae, Discotettigidae, Cladonotidae, Selimenidae, Metrodoridae, and Tetrigidae.

[0213] The superfamily Chorotypidae includes Chorotypidae, Episactidae, Eumastacidae, Euschmidtiidae, Mastacideidae, Morabidae, and Promastacidae. All animals, including those in the families Proscopiidae and Thericleidae.

[0214] The superfamily Acrididae includes all animals such as Pamphagidae, Catantopidae, Arcypteridae, Oedipodids, Acardidae, Pyrgomorphidae, Chrotogonidae, Lathiceridae, Lentulidae, Pneumoridae, and Proscopiidae.

[0215] The superfamily Acrididae includes the genera *Haplofropis*, *Asiotmethis* Uvarov, *Filchnerella* Karny, *Pseudotmethis* Bei-Bienko, *Eotmethis*, *Eoeotmethis*, *Rhinotmethis*, *Sinotmethis*, *Beybienkia*, *Mongolotmethis*, *Thrinchus*, *Aularches Stal*, *Phymateus Thunberg*, *Chrotogonus*, *Pseudomorphacris*, *Tagasta*, *Yunnanites*, *Mekongiana*, *Mekongiella*, *Pyrgomorpha*, and *Atractomorpha sp.*The genera *Sinotmethis*, *Tristria*, *Oxyrrhepes*, *Tauchira*, *Oxytauchira*, *Sinstauchira*, *Pyramisternum*, *Fer*, *Toacris*, *Gesonula*, *Paratoacris*, *Stolzia*, *Oxya*, *Oxyoides*, *Chondracris*, *Schistocerca*, *Catantops*, *Scintharista*, *Gastrimargus*, *Locusta*, *Stenobothrus*, *Epacromius*, *Aiolopus Fieber*, *Mecostethus*, *Oedaleus*, *Pyrgodera*, and *Celes* are mentioned. All animals including *Saussure*, *Oedipoda Latreille*, *Hyalorrhipis*, *Ceracris*, *Formosacris*, *Ceracrisoides*, *Squamopenna Lian et Zheng*, *Ruganotus*, *Arcyptera*, *Xinjiangacris*, *Stenobothrus*, *Egnatioides*, *Gomphoceroides*, *Aeropus*, *Dasyhipps*, *Mesasippus*, *Leptacris*, *Truxalis*, *Phlaeobida*, and *Acrida*.

[0216] The locusts described in this invention include all animals belonging to the superfamily Tetrigoidea, Eustacoidea, and Acridoidea; preferably, they include the following species: Locusta migratoria manilensis, Locusta migratoria L., Locusta migratoria tibetensis Chen, Oedaleus decorus asiaticus, Bryodema luctuosum, Myrmeleotettix Palpalis, Dasyhippus barbipes, Angaracris barabensis Pallas, Chorthippus dubius, Altichorthippus fallax, Angaracris rhodopa, Bryodema tuberculatum dilutum, and Oedaleus. The following locusts are preferred: *Calliptamusitalicus*, *Pararcyptera microptera meridionalis*, *Aiolopus tamulus*, *Shirakiacrisshirakii*, *Xenocatantops brachycerus*, *Oxya chinensis*, *Hieroglyphus annulicornis*, *Epacromius coerulipes*, *Calliptamus abbreviatus Ikonn.*, *Haplofropis Haplotropis neimongolensis*, *Atractomorpha sp.*, *Chondracris rosea*, *Ceracris kiangsu Tsai*, and *Gomphocerus sibiricus sibiricus*; further preferred are the East Asian migratory locust, the Asian migratory locust, the Tibetan migratory locust, the East Asian migratory locust, the Tibetan migratory locust, the East Asian migratory locust, the Chinese rice locust, and the yellow-spined bamboo locust.

[0217] The phylum of nematodes includes those belonging to the following orders: Monohysterida, Dioctophymida, Dorylainida, Merithida, Monochida, Trichocephalida, Enoplida, Rhadditda, Strongylida, Ascaridida, Camallanida, Oxyurida, Spirurida, Diplogasterida, Aphelenchida, Tylenchida, Araeolaimida, Chromadorida, Desmodorida, and Desmocolecida.

[0218] The nematodes include: root-knot nematodes, such as *Meloidogyne incognita* (southern root-knot nematode), *Meloidogyne hapla* (northern root-knot nematode), *Meloidogyne arenaria* (peanut root-knot nematode), and *Meloidogyne javanica* (Javanese root-knot nematode); cyst nematodes, such as *Heterodera glycines Ichinohe* (soybean cyst nematode), *H. avenae* (grass cyst nematode), *H. filipjevi* (salicornia cyst nematode), *H. schachtii* (beet cyst nematode), and *H. goettingiana* (pea cyst nematode); stem nematodes, such as *Ditylenchus dipsaci (Kuhn) Filip.* (sweet potato stem nematode) and *Ditylenchus angustus (Bütler) Filipjev* (rice stem nematode); *T. semipene trans Cobb* (semi-piercing nematode); and leaf nematodes, including *Apholonchoides* (chrysanthemum leaf nematode). Rice nematode Aphelenchoides besseyi Christie; pine wilt nematode Bursaphelenchus xylophilus, etc.

[0219] The nematode is preferably the pine wood nematode.

[0220] The harmful plants described in this invention refer to plants that, under certain conditions, pose a threat to human life, production, and even survival. Harmful agricultural and forestry plants mainly include weeds and invasive plants, specifically including barnyard grass, lambsquarters, amaranth, wild oats, crabgrass, foxtail grass, reeds, caltrop, water sedge, purslane, purple-stemmed eupatorium, Spartina alterniflora, alligator weed, water hyacinth, ragweed, poisonous wheat, airplane grass, Mikania micrantha, golden bell vine, false sorghum, wedelia triloba, five-clawed golden dragon, Italian cocklebur, and black nightshade, etc.

[0221] The harmful microorganisms described in this invention refer to microorganisms that, under certain conditions, can harm human life, production, and even survival. Harmful microorganisms in agriculture and forestry mainly refer to unwanted microorganisms that cause or potentially cause damage to agriculture and forestry.

[0222] Example 1: Experiment with the East Asian migratory locust

[0223] I. Experiment on molting hormone shortening the developmental period of the East Asian migratory locust

[0224] Main experimental materials: 98% ecdysone (main component is 20-hydroxyecdysone, Shaanxi Jinkangtai Biotechnology Co., Ltd.), 2nd instar Oriental migratory locust test insects (Oriental migratory locust egg masses from Yunnan Province were incubated and cultured under uniform laboratory conditions), and corn leaves (grown in the laboratory).

[0225] Preparation of the drug: Dilute the ecdysone with water to a concentration of 0.2 μg / mL ecdysone aqueous solution.

[0226] Corn leaves in the treatment group: Fresh corn leaves were completely immersed in a 0.2 μg / mL ecdysone aqueous solution for 10 seconds, and then removed and air-dried.

[0227] Feeding conditions: Temperature 25-30℃, humidity 28-32%.

[0228] Treatment group: One hundred second-instar Oriental migratory locusts were individually housed in 30×30×30cm wire mesh cages and fed a suitable amount of fresh corn leaves daily. On the first day after the locusts naturally molted (becoming third-instar larvae), they were fed a suitable amount of corn leaves. From the second day onwards, all locusts in the treatment group were fed fresh corn leaves. Molting was observed and recorded at 9:00 AM and 5:00 PM daily. The developmental stages from the third to the fourth instar were statistically analyzed and compared.

[0229] Control group: 100 second-instar Oriental migratory locusts were individually housed in 30×30×30cm wire mesh cages and fed fresh corn leaves once a day. Starting from the first day after the test insects naturally molted (i.e., became third-instar insects), the molting process was observed and recorded at 9:00 and 17:00 every day. The developmental period from the third to the fourth instar was then statistically analyzed and compared.

[0230] Corn husk feeding amount: about 1g / bird each time, once a day.

[0231] Experimental results are as follows Figure 1 As shown, Figure 1 The horizontal axis represents days 1-11 after natural molting in the second instar Oriental migratory locust, and the vertical axis represents the molting rate of the Oriental migratory locust. Figure 1 The results show that the molting time of locusts in the control group started on the 5th day after the molting of the 3rd instar, while that of locusts in the treatment group started on the 4th instar on the 4th day after the molting of the 3rd instar (i.e., the 3rd day after feeding the treatment group corn leaves). The average molting time of locusts in the treatment group was 1 day earlier than that of the control group. Moreover, when the control group just started molting on the 5th day after the molting of the 3rd instar, the molting rate of the treatment group had already reached 13.8%. On the 6th day after the molting of the 3rd instar, the molting rate of the treatment group was 38.5%, which was about twice that of the control group. It was not until the 7.5th day that the molting rates of both the treatment and control groups reached about 60%. This indicates that feeding locusts with a molting hormone solution at a concentration of 0.2 μg / mL can accelerate the growth and development of more than half of the Oriental migratory locusts and shorten the development period.

[0232] II. Experiment on the toxicity of azadirachtin enhanced by ecdysone to the Oriental migratory locust

[0233] Main experimental materials: 98% ecdysone (Shaanxi Jinkangtai Biotechnology Co., Ltd.), 2nd instar Oriental migratory locust test insects (Oriental migratory locust egg masses from Yunnan Province were incubated and cultured under uniform laboratory conditions), azadirachtin technical (mass concentration 10%, Xi'an Ruilin Biotechnology Co., Ltd.), and corn leaves (laboratory-grown).

[0234] Pharmaceutical preparation:

[0235] Azadirachtin solution: Prepare a 0.5% emulsifiable concentrate of azadirachtin technical (formula: 5% by mass of agricultural emulsion 500 + 601, 90% by mass of methyl oleate, with the remainder being azadirachtin technical, wherein the mass ratio of agricultural emulsion 500 and 601 is 1:1). Dilute with water to a concentration of 10 ppm of active ingredient before use.

[0236] Ecdysone aqueous solution: Ecdysone is diluted with water to a concentration of 0.2 ppm.

[0237] Feeding conditions: Temperature 25-30℃, humidity 28-32%.

[0238] Treatment group corn leaves: Fresh corn leaves were completely immersed in a 0.2 ppm ecdysone aqueous solution for 10 seconds, then removed and air-dried. At this time, the mass of ecdysone on 1 g of corn leaves was approximately 0.02 μg.

[0239] Preparation of test insects: Select healthy, undamaged second-instar East Asian migratory locusts of similar size and growth. Keep each second-instar East Asian migratory locust individually in a 30×30×30cm wire mesh cage and feed them fresh corn leaves.

[0240] Experiment 1:

[0241] Treatment 1 (0-day interval): On the first day after the test insects molted naturally, the treatment group was fed 1g of corn leaves. On the first day after molting, the test insects were evenly sprayed with azadirachtin solution (the time interval between feeding the treatment group corn leaves and using azadirachtin solution was 0 days). The amount of azadirachtin solution used for each test insect was about 1ml.

[0242] Azadirachtin control group: On the first day after the test insects molted naturally, the test insects were evenly sprayed with azadirachtin solution.

[0243] Ecdysone control group: 1g of corn leaves were fed to the treatment group on the 1st day after the test insects molted naturally;

[0244] Blank control group: No treatment was performed.

[0245] Experiment 2:

[0246] Treatment 2 (1-day interval): On the 1st day after the test insects molted naturally, the treatment group was fed 1g of corn leaves. On the 2nd day after molting, the test insects were evenly sprayed with azadirachtin solution (the interval between feeding the treatment group corn leaves and using azadirachtin solution was 1 day). The amount of azadirachtin solution used for each test insect was about 1ml.

[0247] Azadirachtin control group: On the second day after the test insects molted naturally, the test insects were evenly sprayed with azadirachtin solution.

[0248] Ecdysone control group: 1g of corn leaves were fed to the treatment group on the 1st day after the test insects molted naturally;

[0249] Blank control group: No treatment was performed.

[0250] Experiment 3:

[0251] Treatment 3 (2-day interval): On the 1st day after the test insects molted naturally, the treatment group was fed 1g of corn leaves. On the 3rd day after molting, the test insects were evenly sprayed with azadirachtin solution (the interval between feeding the treatment group corn leaves and using azadirachtin solution was 2 days). The amount of azadirachtin solution used for each test insect was about 1ml.

[0252] Azadirachtin control group: On the 3rd day after the test insects molted naturally, the test insects were evenly sprayed with azadirachtin solution.

[0253] Ecdysone control group: 1g of corn leaves were fed to the treatment group on the 1st day after the test insects molted naturally;

[0254] Blank control group: No treatment was performed.

[0255] Experiment 4:

[0256] Treatment 4 (3-day interval): On the 1st day after the test insects molted naturally, the treatment group was fed 1g of corn leaves. On the 4th day after molting, the test insects were evenly sprayed with azadirachtin solution (the interval between feeding the treatment group corn leaves and using azadirachtin solution was 3 days). The amount of azadirachtin solution used for each test insect was about 1ml.

[0257] Azadirachtin control group: On the 4th day after the test insects molted naturally, the test insects were evenly sprayed with azadirachtin solution.

[0258] Ecdysone control group: 1g of corn leaves were fed to the treatment group on the 1st day after the test insects molted naturally;

[0259] Blank control group: No treatment was performed.

[0260] Experiment 5:

[0261] Treatment 5 (4-day interval): On the 1st day after the test insects molted naturally, the treatment group was fed 1g of corn leaves. On the 5th day after molting, the test insects were evenly sprayed with azadirachtin solution (the interval between feeding the treatment group corn leaves and using azadirachtin solution was 4 days). The amount of azadirachtin solution used for each test insect was about 1ml.

[0262] Azadirachtin control group: On the 5th day after the test insects molted naturally, the test insects were evenly sprayed with azadirachtin solution.

[0263] Ecdysone control group: 1g of corn leaves were fed to the treatment group on the 1st day after the test insects molted naturally;

[0264] Blank control group: No treatment was performed.

[0265] Experiment 6:

[0266] Treatment 6 (5-day interval): On the 1st day after the test insects molted naturally, the treatment group was fed 1g of corn leaves. On the 6th day after molting, the test insects were evenly sprayed with azadirachtin solution (the interval between feeding the treatment group corn leaves and using azadirachtin solution was 5 days). The amount of azadirachtin solution used for each test insect was about 1ml.

[0267] Azadirachtin control group: On the 6th day after the test insects molted naturally, the test insects were evenly sprayed with azadirachtin solution.

[0268] Ecdysone control group: 1g of corn leaves were fed to the treatment group on the 1st day after the test insects molted naturally;

[0269] Blank control group: No treatment was performed.

[0270] Each treatment consisted of 20 Oriental migratory locusts, with 5 replicates. The mortality rate of the Oriental migratory locusts within 72 hours after each spraying of azadirachtin solution was recorded, and the average value was calculated.

[0271] Corn husk feeding amount: 1g / bird each time, once a day.

[0272] The mortality rates of the Oriental migratory locusts in the above experiments are shown in Table 1 and... Figure 2 Table 1 shows the synergistic effect of alternating use of ecdysone and azadirachtin on the killing of East Asian migratory locusts, and the results are shown in Table 2.

[0273] Table 1. Poisoning effect of each experimental group on the Oriental migratory locust.

[0274]

[0275]

[0276] Table 2. Synergistic effect of alternating use of ecdysone and azadirachtin on the killing of East Asian migratory locusts.

[0277] Interval between use of ecdysone and azadirachtin 0d 1d 2d 3d 4d 5d Expected mortality rate (%) 37.29 49.75 32.77 47.84 37.08 37.38 Observe the mortality rate (%) 31.25 39.06 66.69 45.00 24.81 37.35 Efficiency improvement rate (%) -16.20 -21.49 103.52 -5.94 -33.09 -0.08

[0278] Note:

[0279] Expected mortality rate: The sum of mortality rates when ecdysone and azadirachtin are used alone;

[0280] Mortality rate observation: Mortality rate observed after the administration of ecdysone and azadirachtin according to the experimental protocol;

[0281] Increase in efficiency: (observed mortality rate - expected mortality rate) / expected mortality rate * 100%.

[0282] from Figure 2 It can be seen that ecdysone has no direct toxic effect on the Oriental migratory locust. When azadirachtin solution was applied to the Oriental migratory locusts in the feeding group on corn leaves containing ecdysone (i.e., simultaneous application of ecdysone and azadirachtin solution), the mortality rate of the treated group was 31.25% within 72 hours, comparable to that of the azadirachtin control group. However, when azadirachtin solution was applied to the Oriental migratory locusts in the feeding group on corn leaves containing ecdysone, the mortality rate of the treated group was 66.69% within 72 hours, compared to 31.25% in the azadirachtin control group. The efficacy of the treated group was 103.52% higher than that of the azadirachtin control group. Meanwhile, at other time intervals, the mortality rates of the treated group and the azadirachtin control group were not significantly different, and the efficacy increases were all less than 0. Therefore, it is inferred that feeding the locusts corn leaves containing molting hormones to the locusts in the feeding group followed by the application of azadirachtin solution 2 days later would significantly enhance the killing effect of azadirachtin on the locusts.

[0283] Example 2: Pine Wood Nematode Experiment

[0284] I. Experiment on the toxicity of dimethyl azelaate, enhanced by ecdysone, against pine wood nematodes.

[0285] Drug preparation:

[0286] Ecdysone aqueous solution: Prepare an aqueous solution of ecdysone at 0.5 ppm;

[0287] Dimethyl azelaate aqueous solution: Prepare a 110 ppm aqueous solution of dimethyl azelaate;

[0288] The experimental group was set up as follows:

[0289] Experiment 1:

[0290] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h by immersion.

[0291] Dimethyl azelate control group: Pine wood nematodes were treated with 1 ml of 110 ppm dimethyl azelate aqueous solution for 24 h by immersion.

[0292] Treatment 1 (0h interval): First, mix 0.5ppm ecdysone aqueous solution and 110ppm dimethyl azelate aqueous solution, take 1ml of the compound solution, and treat pine wood nematodes by immersion method for 24h;

[0293] CK (water): Pine wood nematode was treated by immersion in clean water for 24 hours.

[0294] Experiment 2:

[0295] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h by immersion.

[0296] Dimethyl azelate control group: Pine wood nematodes were treated with 1 ml of 110 ppm dimethyl azelate aqueous solution for 24 h by immersion.

[0297] Treatment 2 (24h interval): First, treat pine wood nematodes with 1ml of 0.5ppm ecdysone aqueous solution for 24h by soaking the insects. After centrifugation to remove the ecdysone aqueous solution, add 1ml of 110ppm dimethyl azelate aqueous solution and treat the insects by soaking the insects for 24h.

[0298] CK (water): Pine wood nematode was treated by water immersion method for 48 hours.

[0299] Experiment 3:

[0300] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h by immersion.

[0301] Dimethyl azelate control group: Pine wood nematodes were treated with 1 ml of 110 ppm dimethyl azelate aqueous solution for 24 h by immersion.

[0302] Treatment 3 (48h interval): First, treat pine wood nematodes with 1ml of 0.5ppm ecdysone aqueous solution for 48h by soaking the insects. After centrifugation to remove the ecdysone aqueous solution, add 1ml of 110ppm dimethyl azelate aqueous solution and treat the insects by soaking the insects for 24h.

[0303] CK (water): Pine wood nematode was treated by water immersion for 72 hours.

[0304] Experiment 4:

[0305] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h by immersion.

[0306] Dimethyl azelate control group: Pine wood nematodes were treated with 1 ml of 110 ppm dimethyl azelate aqueous solution for 24 h by immersion.

[0307] Treatment 4 (72h interval): Treatment 3: First, treat pine wood nematodes with 1ml of 0.5ppm ecdysone aqueous solution for 72h. After centrifugation to remove the ecdysone aqueous solution, add 1ml of 110ppm dimethyl azelate aqueous solution and treat for 24h.

[0308] CK (water): Pine wood nematode was treated with water immersion method for 96 hours.

[0309] Preparation of nematode suspension: Pine wood nematodes on corn kernel culture medium were suspended in the aqueous phase with an appropriate amount of sterile water. The live pine wood nematode suspension was then separated by the Bellman funnel method. The nematode suspension was centrifuged at 2000 r / min for 2 min, the supernatant was removed, and an appropriate amount of sterile water was added. The nematode age and activity were observed under a microscope, and the number of nematodes was counted. Finally, the nematode suspension was quantified to 10,000 nematodes / mL with sterile water. The vast majority of nematodes were in the J3 and J4 stages, and a few were in the J2 stage. The suspension was stored at 4℃.

[0310] Treatment: Take 0.5 mL of the nematode suspension prepared above, centrifuge at 2,000 r / min for 2 min, remove the supernatant, add 1 mL of the solutions prepared above to the precipitate, mix well with a pipette, cap the centrifuge tubes, and incubate in a dark, artificial climate chamber at 25℃ and 70% relative humidity. Subsequent operations are performed according to the different treatments described above. Observe and record the total number of nematodes and the number of dead nematodes under an Olympus CX33 optical microscope.

[0311] Each experimental group was repeated three times, and the average value was taken. The mortality rate of pine wood nematodes and the synergistic effect of ecdysone on dimethyl azelate in each group are shown in Tables 3 and 4, respectively.

[0312] Table 3. The toxic effects of each experimental group on pine wood nematode.

[0313]

[0314] Table 4. Synergistic effect of alternating use of ecdysone and dimethyl azelate on the killing of pine wilt nematodes.

[0315]

[0316] Note:

[0317] Expected mortality rate: The sum of mortality rates when ecdysone and dimethyl azelaate are used alone;

[0318] Mortality rate observation: Mortality rate observed after the administration of ecdysone and dimethyl azelaate according to the experimental protocol;

[0319] Increase in efficiency: (observed mortality rate - expected mortality rate) / expected mortality rate * 100%;

[0320] Based on Table 3, draw the following diagram. Figure 3 From the table above and Figure 3 The results show that: 0.5 ppm ecdysone alone has no toxic activity against pine wood nematodes; simultaneous treatment of pine wood nematodes with 0.5 ppm ecdysone and 110 ppm dimethyl azelaate does not significantly enhance the toxic activity compared to 110 ppm dimethyl azelaate alone; however, after treating pine wood nematodes with 0.5 ppm ecdysone for 24 h, 48 h, and 72 h, followed by treatment with the nematicidal compound dimethyl azelaate for 24 h, the toxic activity against pine wood nematodes is significantly increased compared to dimethyl azelaate alone, with an increase of 45%–49%. This indicates that treating pine wood nematodes with ecdysone for a period of time followed by treatment with the nematicidal compound dimethyl azelaate significantly increases the toxic activity of the nematicidal compound against pine wood nematodes. II. Ecdysone-enhanced toxicity experiment of ethyl paraben against pine wood nematodes.

[0321] Drug preparation:

[0322] Ecdysone aqueous solution: Prepare an aqueous solution of ecdysone at 0.5 ppm;

[0323] Ethyl paraben aqueous solution: Prepare an aqueous solution of ethyl paraben at 500 ppm;

[0324] The experimental group was set up as follows:

[0325] Experiment 1:

[0326] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h using the immersion method.

[0327] Ethylparaben control group: Pine wood nematodes were treated with 1 ml of 500 ppm ethylparaben aqueous solution for 24 h using the immersion method.

[0328] Treatment 1 (0h interval): First, mix 0.5ppm ecdysone aqueous solution and 500ppm ethylparaben aqueous solution, take 1ml of the compound solution and treat pine wood nematodes for 24h using the immersion method;

[0329] CK (water): Soak pine wood nematodes in clean water for 24 hours.

[0330] Experiment 2:

[0331] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h using the immersion method.

[0332] Ethylparaben control group: Pine wood nematodes were treated with 1 ml of 500 ppm ethylparaben aqueous solution for 24 h using the immersion method.

[0333] Treatment 2 (10-hour interval): Pine wood nematodes were first treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 10 hours, and after centrifugation to remove the ecdysone, 1 ml of 500 ppm ethylparaben aqueous solution was added for 24 hours.

[0334] CK (water): Pine wood nematode was treated by water immersion method for 48 hours.

[0335] Experiment 3:

[0336] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h using the immersion method.

[0337] Ethylparaben control group: Pine wood nematodes were treated with 1 ml of 500 ppm ethylparaben aqueous solution for 24 h using the immersion method.

[0338] Treatment 3 (24h interval): First, treat pine wood nematodes with 1ml of 0.5ppm ecdysone aqueous solution for 24h by soaking. After centrifugation to remove the ecdysone aqueous solution, add 1ml of 500ppm ethylparaben aqueous solution and treat for another 24h by soaking.

[0339] CK (water): Soak pine wood nematodes in clean water for 72 hours.

[0340] Experiment 4:

[0341] Ecdysone control group: Pine wood nematodes were treated with 1 ml of 0.5 ppm ecdysone aqueous solution for 24 h using the immersion method.

[0342] Ethylparaben control group: Pine wood nematodes were treated with 1 ml of 500 ppm ethylparaben aqueous solution for 24 h using the immersion method.

[0343] Treatment 4 (48h interval): First, treat pine wood nematodes with 1ml of 0.5ppm ecdysone aqueous solution for 48h by soaking. After centrifugation to remove the ecdysone aqueous solution, add 1ml of 500ppm ethylparaben aqueous solution and treat for 24h by soaking.

[0344] CK (water): Soak pine wood nematodes in clean water for 96 hours.

[0345] Preparation of nematode suspension: Pine wood nematodes on corn kernel culture medium were suspended in the aqueous phase with an appropriate amount of sterile water. The live pine wood nematode suspension was then separated by the Bellman funnel method. The nematode suspension was centrifuged at 2000 r / min for 2 min, the supernatant was removed, and an appropriate amount of sterile water was added. The nematode age and activity were observed under a microscope, and the number of nematodes was counted. Finally, the nematode suspension was quantified to 10,000 nematodes / mL with sterile water. The vast majority of nematodes were in the J3 and J4 stages, and a few were in the J2 stage. The suspension was stored at 4℃.

[0346] Treatment: Take 0.5 mL of the nematode suspension prepared above, centrifuge at 2,000 r / min for 2 min, remove the supernatant, add 1 mL of the solutions prepared above to the precipitate, mix well with a pipette, cap the centrifuge tubes, and incubate in a dark, artificial climate chamber at 25℃ and 70% relative humidity. Subsequent operations are performed according to the different treatments described above. Observe and record the total number of nematodes and the number of dead nematodes under an Olympus CX33 optical microscope.

[0347] Each experimental group was repeated three times, and the average value was taken. The mortality rate of pine wood nematodes and the synergistic effect of ecdysone on ethylparaben are shown in Tables 5 and 6, respectively.

[0348] Table 5. The toxic effects of each experimental group on pine wood nematode.

[0349]

[0350] Table 6. Synergistic effect of alternating use of ecdysone and ethylparaben on the control of pine wilt nematode.

[0351]

[0352] Note:

[0353] Expected mortality rate: The sum of mortality rates when ecdysone and ethylparaben are used alone;

[0354] Mortality rate observation: Mortality rate observed after the administration of ecdysone and ethylparaben according to the experimental protocol;

[0355] Increase in efficiency: (observed mortality rate - expected mortality rate) / expected mortality rate * 100%.

[0356] Table 5 can be used to draw the following: Figure 4 From the table above and Figure 4 The results show that: 0.5 ppm ecdysone alone has no toxic activity against pine wood nematodes; simultaneous treatment of pine wood nematodes with 0.5 ppm ecdysone and ethylparaben does not significantly enhance the toxic activity compared to ethylparaben alone; however, treatment of pine wood nematodes with 0.5 ppm ecdysone at intervals of 10 h, 24 h, and 48 h, followed by treatment with the nematicidal compound ethylparaben for 24 h, significantly increases the toxic activity against pine wood nematodes compared to dimethyl azelaate alone, with increases ranging from 22% to 175%. This indicates that treating pine wood nematodes with ecdysone for a period of time followed by treatment with the nematicidal compound ethylparaben significantly increases the toxic activity of the nematicidal compound against pine wood nematodes.

Claims

1. A composition for controlling unintended organisms, characterized in that: The composition consists of two parts, component A and component B, with a mass ratio of component A to component B of (1:10). -3 ~1):1, wherein: component A is ecdysone, component B is azadirachtin, ethylparaben or dimethyl azelaate; and the composition is applied in sequence.

2. The composition for controlling unintended organisms according to claim 1, characterized in that: In the composition, components A and B are applied in a sequential order, with component A added first and component B added later, with an interval of 6 to 144 hours between them.

3. The application of the composition for controlling unintended organisms as described in claim 1, characterized in that: The application of the composition in inhibiting unintended organisms, which are unwanted organisms.

4. A compositional formulation for controlling unintended biological organisms, characterized in that: The formulation is prepared by using the composition described in claim 1.

5. A method for controlling unintentional organisms, characterized in that: The composition of claim 1 is applied to unintended organisms, wherein the time interval between the application of component A and component B is 6 to 144 hours.

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