A fungicidal composition containing prothioconazole and thiram and its use

Abstract

The present application provides a kind of fungicidal composition containing prothioconazole and thiram, comprising active ingredient and synergist, wherein active ingredient is composed of prothioconazole and thiram, and the mass ratio of prothioconazole and thiram is 1:5-5:1;Synergist is one or more of salicylic acid, oxalic acid or citric acid.The fungicidal composition of the present application has significant synergistic effect, and the addition of synergist (salicylic acid, oxalic acid, citric acid) in the composition can significantly improve the control effect of the fungicide on wheat scab and wheat powdery mildew, effectively reduce the dosage of active ingredient, slow down the development of drug resistance of pathogen, reduce environmental pollution and ensure food safety.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of pesticides, in particular to a fungicidal composition containing propiconazole and thiram and application thereof. BACKGROUND

[0002] For a long time, the unreasonable use of chemical pesticides has brought a series of difficult problems such as pesticide residues exceeding the standard in agricultural products, pollution of ecological environment, occurrence of pest resistance and threat to human health. At the same time, due to the high cost, high risk and long cycle of new pesticide synthesis, it is difficult to meet the demand for efficient and safe pesticides in a short period. The research on pesticide synergistic composition and pesticide dosage form is an important aspect of pesticide development, which can reduce the use of pesticides and facilitate the use of suitable pesticide formulations, thereby reducing the impact on the environment.

[0003] Propiconazole is a triazole thione fungicide, which belongs to the inhibitor of sterol demethylation (biosynthesis of ergosterol). It has the characteristics of selectivity, protection, treatment and persistence. Thiram is a thiocarbamate fungicide with protective and therapeutic effects. The active ingredient moves with the carrier into the disease tissue and the inside of the fungus, thereby playing a good fungicidal effect. Guangdong Zhixun Agricultural Science and Technology Co., Ltd. (201210119920.6) discloses a pesticide composition containing propiconazole and thiram, the weight ratio of propiconazole and thiram is 1:30-20:1, which can be used for the prevention and control of cucumber anthracnose. However, the effect of the composition on the prevention and control of other crop diseases, especially wheat scab and powdery mildew, is not disclosed. SUMMARY

[0004] The purpose of the present application is to provide a fungicidal composition with significant synergistic effect. Another purpose of the present application is to improve the effect of the composition by adding suitable synergists.

[0005] The present application provides the following technical solutions:

[0006] A fungicidal composition containing propiconazole and thiram, characterized in that it comprises active ingredients and synergists, wherein the active ingredients consist of propiconazole and thiram, and the mass ratio of propiconazole to thiram is 1:5-5:1; the synergist is one or more of salicylic acid, oxalic acid or citric acid.

[0007] Preferably, the mass ratio of propiconazole to thiram is 4:1-2:1. Particularly preferably, the mass ratio of propiconazole to thiram is 3:1.

[0008] Preferably, the synergist consists of oxalic acid and citric acid, and the mass ratio of oxalic acid to citric acid is 1:3-3:1. Particularly preferably, the mass ratio of oxalic acid to citric acid is 1:1.

[0009] The preparation of the fungicidal composition is a suspension agent, and further comprises an agricultural adjuvant ingredient.

[0010] Preferably, the 40% propiconazole and thiram suspension agent comprises the following ingredients by weight percentage: propiconazole 25%, thiram 15%, oxalic acid 1.5%, citric acid 1.5%, sodium dodecyl benzene sulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organic bentonite 1.5%, dimethyl silicone oil 0.5%, and deionized water to 100%.

[0011] The fungicidal composition is used for the prevention and treatment of wheat scab and wheat powdery mildew.

[0012] By adopting the technical scheme, the present application has the following beneficial effects:

[0013] The fungicidal composition has a significant synergistic effect, and the addition of the synergist (salicylic acid, oxalic acid, and citric acid) in the composition can significantly improve the prevention and treatment effect of the fungicide on wheat scab and wheat powdery mildew, effectively reduce the use dosage of the active ingredient, slow down the generation of drug resistance of the pathogen, reduce environmental pollution, and ensure food safety. DETAILED DESCRIPTION

[0014] Based on the examples in the present application, all other examples obtained by a person of ordinary skill in the art without making creative efforts fall within the protection scope of the present application.

[0015] The present application will be further explained and described in combination with specific embodiments.

[0016] Example 1: Test on the combined effect of propiconazole and thiram on wheat scab

[0017] Test strain: wheat scab pathogen (Fusarium graminearum), collected in the field and identified and cultured in the laboratory for preservation.

[0018] Test agent: propiconazole and thiram

[0019] Test method: The mycelial growth rate method was adopted for the indoor toxicity determination of wheat scab. According to the results of the pre-test, 7 concentration gradients of the test agent were selected, the prepared test agent was added to the sterilized and cooled PDA medium at 55℃ in proportion, and then poured into a 9cm sterilized culture dish, and then a 5mm fungus cake was inoculated, 4 repetitions were set for each treatment, and then sealed and placed in a 28℃ constant temperature box for culture. After 5 days, the cross method was adopted to measure the colony diameter, and the mycelial growth inhibition rate was calculated.

[0020] The mycelium growth inhibition rate = (control colony growth diameter - treatment colony growth diameter) / (control colony growth diameter - mycelium cake diameter) x 100%.

[0021] The mycelium growth inhibition rate of the colony was converted into inhibition probability as the dependent variable (y), and each concentration of the agent (mg / L) was converted into logarithmic value with base 10 (x), and the effective inhibition median concentration EC50 of each test agent was calculated by DPS software.

[0022] According to Sun Yunpei method, the actual toxicity index (ATI), the theoretical toxicity index (TTI) and the co-toxicity coefficient (CTC) of the compound agent were calculated to evaluate the synergistic effect and determine the best ratio.

[0023] The single agent toxicity index = (EC50 of the standard agent / EC50 of the single agent) x 100;

[0024] The theoretical toxicity index (TTI) = the toxicity index of single agent A x the proportion of single agent A in the mixture + the toxicity index of single agent B x the proportion of single agent B in the mixture;

[0025] The actual toxicity index (ATI) = (EC50 value of the standard single agent / EC50 value of the mixture) x 100;

[0026] The co-toxicity coefficient (CTC) = (actual toxicity index / theoretical toxicity index) x 100;

[0027] When the CTC is greater than 120, the mixture has synergistic effect, when the CTC is less than 80, it is antagonistic, and when the CTC is between 80-120, it is additive effect.

[0028] Results and analysis:

[0029] As shown in Table 1, prothioconazole and thiram have excellent control effect on wheat scab, and the EC50 values are 6.5618 and 13.4205 mg / L, respectively. When the mass ratio of prothioconazole and thiram is 3:1, the compound composition shows significant synergistic effect on wheat scab, and the CTC value is 138.2. When the composition shows synergistic effect, it can effectively reduce the dosage of active ingredients and slow down the development of drug resistance of the pathogen.

[0030] Table 1 Synergistic effect of prothioconazole and thiram on wheat scab

[0031]

[0032] Example 2 Formulation

[0033] Tested materials: prothioconazole technical material, thiram technical material, sodium dodecyl benzene sulfonate, Ethylan NS 500LQ, Agrlian-700, ethylene glycol, sodium benzoate, magnesium aluminum silicate, xanthan gum, organic bentonite, synergist (salicylic acid, oxalic acid, citric acid), dimethyl silicone oil, deionized water.

[0034] Main experimental instruments: electronic balance, CX31 microscope, JL-1156 laser particle size distribution tester, sand mill, etc.

[0035] Preparation method: the pesticide dosage form is prepared by mixing the technical material and excipients in a certain proportion with water as the dispersion medium and performing wet ultrafine grinding. When grinding, zirconium oxide beads are added to the mixture, and the specific gravity is 2 times the weight of the suspension itself. At room temperature, high-speed grinding is performed at a speed of 1200 r / min, and the average particle size of the suspension is reduced to less than 5 μm, and the pesticide suspension can be obtained after separating the zirconium beads.

[0036] The specific formula is as follows:

[0037] Formula 1: 40% prothioconazole-thiram suspension concentrate, containing the following ingredients by weight percentage:

[0038] Prothioconazole 25%, thiram 15%, synergist (salicylic acid) 3%, sodium dodecyl benzene sulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organic bentonite 1.5%, dimethyl silicone oil 0.5%, deionized water to 100%.

[0039] Formula 2: 40% prothioconazole-thiram suspension concentrate, containing the following ingredients by weight percentage:

[0040] Prothioconazole 25%, thiram 15%, synergist (oxalic acid) 3%, sodium dodecyl benzene sulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organic bentonite 1.5%, dimethyl silicone oil 0.5%, deionized water to 100%.

[0041] Formula 3: 40% prothioconazole-thiram suspension concentrate, containing the following ingredients by weight percentage:

[0042] Propiconazole 25%, Thiram 15%, synergist (salicylic acid) 3%, sodium dodecylbenzenesulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organobentonite 1.5%, dimethicone 0.5%, deionized water to 100%.

[0043] Formulation 4: 40% Propiconazole-Thiram SC, comprising the following ingredients in weight percent:

[0044] Propiconazole 25%, Thiram 15%, synergist (salicylic acid + oxalic acid mass ratio = 1 : 1) 3%, sodium dodecylbenzenesulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organobentonite 1.5%, dimethicone 0.5%, deionized water to 100%.

[0045] Formulation 5: 40% Propiconazole-Thiram SC, comprising the following ingredients in weight percent:

[0046] Propiconazole 25%, Thiram 15%, synergist (salicylic acid + oxalic acid mass ratio = 1 : 1) 3%, sodium dodecylbenzenesulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organobentonite 1.5%, dimethicone 0.5%, deionized water to 100%.

[0047] Formulation 6: 40% Propiconazole-Thiram SC, comprising the following ingredients in weight percent:

[0048] Propiconazole 25%, Thiram 15%, synergist (salicylic acid + oxalic acid mass ratio = 1 : 1) 3%, sodium dodecylbenzenesulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organobentonite 1.5%, dimethicone 0.5%, deionized water to 100%.

[0049] Formulation 7: 40% Propiconazole-Thiram SC, comprising the following ingredients in weight percent:

[0050] Prothioconazole 25%, Thiram 15%, Sodium Dodecylbenzenesulfonate 2%, Ethylan NS 500LQ 3%, Agrlian-700 5%, Ethylene glycol 2%, Sodium Benzoate 0.5%, Magnesium Aluminum Silicate 1%, Xanthan Gum 0.5%, Organobentonite 1.5%, Dimethicone 0.5%, Deionized water to 100%.

[0051] Example 3 Field efficacy test of 40% Prothioconazole-Thiram SC against wheat scab

[0052] Test crop: Wheat

[0053] Crop disease: Scab

[0054] Test agent: Formulation 1-7

[0055] Test method: Wheat scab: According to the People's Republic of China industry standard NY / T 1464.15-2007 "Guidelines for Pesticide Field Efficacy Tests Part 15: Fungicides for Controlling Wheat Scab", in winter wheat field, the first spraying was performed at the early flowering stage, and the second spraying was performed 7 days later, a total of 2 times, with a water volume of 30 L / acre, and the investigation time was 21 days after the second spraying.

[0056] Results and analysis

[0057] The addition of synergists (salicylic acid, oxalic acid, citric acid) in 40% Prothioconazole-Thiram SC can significantly improve the control effect of prothioconazole and thiram complex suspension on wheat scab; and when the synergists are combined as oxalic acid and citric acid, the control effect on wheat scab is 96.54% at the same dosage, which can significantly reduce the field dosage of active ingredients, reduce environmental pollution, and ensure food safety.

[0058] Table 2 Field efficacy of 40% Prothioconazole-Thiram SC against wheat scab

[0059]

[0060]

[0061] Example 4 Field efficacy test of 40% Prothioconazole-Thiram SC against wheat powdery mildew

[0062] Test crop: Wheat

[0063] Crop disease: Powdery mildew

[0064] Test agent: Formulation 1-7

[0065] Test method: Select the early stage of wheat powdery mildew in the field for spraying treatment, spray 2 times with an interval of 7 days, and the spraying time is about late April, with a water volume of 30 L / acre.

[0066] Investigation time: the disease base investigation was carried out before drug administration, and the prevention effect investigation was carried out 14 days after drug administration twice; investigation method: 5 points sampling per plot, 20 wheat plants were investigated per point, and disease classification was carried out.

[0067] Wheat powdery mildew incidence classification: 0 level: no disease; 1 level: the diseased area accounts for less than 5% of the whole leaf area; 3 level: the diseased area accounts for 6%-15% of the whole leaf area; 5 level: the diseased area accounts for 16%-25% of the whole leaf area; 7 level: the diseased area accounts for 26%-50% of the whole leaf area; 9 level: the diseased area accounts for more than 50% of the whole leaf area.

[0068] Calculation formula:

[0069]

[0070]

[0071] In the formula: CK0 is the disease index before drug administration in the blank control area; CK1 is the disease index after drug administration in the blank control area; PT0 is the disease index before drug administration in the drug treatment area; PT1 is the disease index before drug administration in the drug treatment area.

[0072] Results and analysis

[0073] The addition of synergistic agents (salicylic acid, oxalic acid and citric acid) in 40% propiconazole and thiram suspension concentrate can significantly improve the control effect of propiconazole and thiram on wheat powdery mildew; and when the synergistic agents are combined as oxalic acid and citric acid, the control effect on wheat powdery mildew is 94.97% at the same dosage, which can significantly reduce the field dosage of active ingredients, reduce environmental pollution and ensure food safety.

[0074] Table 3 Field control effect of 40% propiconazole and thiram suspension concentrate on wheat powdery mildew

[0075]

[0076] Finally, it should be pointed out that: the above examples are only used to illustrate the technical solutions of the present application, and not to limit them.

Claims

1. A fungicidal composition comprising ipconazole and thiram, characterized in that, The fungicidal composition is 40% propiconazole + thiram suspension concentrate, comprising the following ingredients by weight percentage: propiconazole 25%, thiram 15%, oxalic acid 1.5%, citric acid 1.5%, sodium dodecyl benzene sulfonate 2%, Ethylan NS500LQ 3%, Agrlian-700 5%, ethylene glycol 2%, sodium benzoate 0.5%, magnesium aluminum silicate 1%, xanthan gum 0.5%, organic bentonite 1.5%, dimethyl silicone oil 0.5%, and deionized water to 100%.

2. Use of the fungicidal composition according to claim 1 for the control of wheat scab and wheat powdery mildew.

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