Bifenthrin hapten, antigen, antibody, preparation method and application thereof
By preparing the bifenthrin hapten and coupling it with a carrier protein, the complexity and high cost of existing bifenthrin detection methods are solved, and a highly specific and sensitive enzyme-linked reaction adsorption analysis method is achieved, which is suitable for the rapid detection of bifenthrin.
Patent Information
- Application Number
- CN202311068459.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2023-08-23
- Publication Date
- 2025-09-12
- Estimated Expiration
- 2043-08-23
AI Technical Summary
Existing methods for detecting cypermethrin mainly rely on instrumental analysis, which has problems such as cumbersome sample processing, high detection costs, expensive equipment, complex operations, and unsuitability for rapid on-site detection. At the same time, the immunogenicity of the antigen in the enzyme-linked reaction adsorption analysis method is not strong, resulting in poor antibody specificity and sensitivity.
A bifenthrin hapten was prepared by Suzuki reaction using p-aminophenylboronic acid, 3-bromo-2-methylbenzyl alcohol, alkaline reagent and palladium catalyst, followed by coupling with trifluorochlorochrysanthemoyl chloride and succinic anhydride. The obtained hapten was coupled with a carrier protein to prepare a bifenthrin antigen with strong immunogenicity for enzyme-linked reaction adsorption analysis.
The specificity and sensitivity of the antibody are improved, efficient and rapid detection of bifenthrin is achieved, the detection cost is reduced, and it is suitable for accurate analysis of large quantities of samples.