Liquid functional dressing for preventing pressure sores, moisturizing skin and repairing skin wounds and preparation method thereof

Abstract

The application discloses a liquid functional dressing for preventing pressure sores, moisturizing skin and repairing skin wounds and a preparation method thereof. The liquid dressing takes corn oil and anise as raw materials, and the preparation method comprises the following steps: crushing the anise, sieving, and obtaining anise powder; mixing the anise powder with petroleum ether, and treating under ultrasonic waves at 35-55 DEG C, filtering, and taking the filtrate; concentrating and removing the solvent from the filtrate, obtaining a concentrate, mixing the concentrate with an aqueous acetone solution, adjusting the pH to be alkaline, treating under ultrasonic waves at 40-60 DEG C, filtering, taking the filtrate, concentrating and removing the solvent, and obtaining an anise extraction concentrate; mixing the corn oil with the anise extraction concentrate, and obtaining the liquid functional dressing. The liquid functional dressing has good moisturizing effect, can promote the growth of new blood vessels and wound healing, and has better wound repair capacity, and can be used for treating pressure ulcers and dry skin.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a liquid functional dressing for preventing pressure ulcers, moisturizing and repairing skin wounds, and its preparation method. Background Technology

[0002] A wound is damage to normal skin tissue caused by external injury factors (such as surgery, external force, high temperature, low temperature, electricity, and chemicals) and internal factors (such as impaired local blood supply). Pressure ulcers are a common type of skin wound. Also known as bedsores, pressure ulcers, or pressure injuries, pressure ulcers are caused by prolonged pressure on a localized area of ​​the skin, affecting blood circulation and leading to malnutrition of the skin and subcutaneous tissue, resulting in damage, ulceration, and even necrosis. External methods for preventing or treating pressure ulcers mainly involve promoting blood vessel growth and blood circulation in the pressure area, keeping the skin moist, and avoiding excessive dryness in the pressure area.

[0003] Currently known liquid dressings use a variety of raw materials, often including some expensive ones such as bioactive glass. This puts pressure on manufacturers, and products made with expensive raw materials typically have higher selling prices. Corn oil is a common edible oil in daily life, rich in linoleic acid, linolenic acid, and vitamin E, and has some wound-healing effects, but its effectiveness is limited and it is difficult to meet people's needs when used alone. Star anise belongs to the Magnoliaceae family and is also known as anise. Its fruit is a well-known spice, with a slightly sweet and slightly spicy taste. The flavor compounds in its fruit are mainly anethole and anisaldehyde. Star anise is widely used in the food industry, but its application as an active ingredient in skin wound repair has not been reported. Summary of the Invention

[0004] In view of this, the present invention provides a liquid functional dressing for preventing pressure sores, moisturizing and repairing skin wounds, and a method for preparing the same.

[0005] The solution of this invention is as follows:

[0006] A method for preparing a liquid functional dressing for preventing pressure ulcers, moisturizing the skin, and repairing skin wounds includes the following steps:

[0007] S1: Pretreatment of anise (i.e., the dried fruit of the anise plant, Magnoliaceae family): Grind the anise into powder, sieve it to obtain anise powder; mix the anise powder with petroleum ether, sonicate it at 35-55℃, filter it, and collect the filtrate; concentrate the filtrate to remove the solvent, obtain the concentrate; mix the concentrate with acetone aqueous solution and adjust the pH to alkaline, sonicate it at 40-60℃, filter it, collect the filtrate, concentrate it to remove the solvent, and obtain the anise extract concentrate;

[0008] S2: Mix corn oil with the anise extract concentrate obtained in step S1 to obtain a liquid functional dressing.

[0009] Preferably, in step S2, the mass ratio of corn oil to anise extract concentrate is 70:20-30.

[0010] Preferably, the alkalinity is pH 7.6 to 8.7.

[0011] Preferably, the concentration of the acetone aqueous solution is 80 v / v% to 90 v / v%.

[0012] Preferably, the ultrasonic treatment time is 3 to 4 hours.

[0013] Preferably, in step S1, the ratio of anise powder to petroleum ether is 1 mg: 10-20 mL.

[0014] Preferably, in step S1, the ratio of the concentrate to the acetone aqueous solution is 1 mg: 10-20 mL.

[0015] Compared with the prior art, the beneficial effects of the present invention are:

[0016] This invention relates to a liquid dressing made from corn oil and star anise, both readily available ingredients. Research has shown that ultrasonic extraction of star anise at 50°C, followed by extraction of its alkali-soluble active ingredients in an acetone aqueous solution at 40–60°C, produces a product with excellent moisturizing properties when mixed with corn oil. This product promotes angiogenesis, wound healing, and overall wound repair.

[0017] The product of this invention can alleviate and repair skin tissue damage caused by pressure, friction, etc., protect damaged skin, has good moisturizing properties, effectively limits moisture loss, prevents dry skin, and can be used for pressure ulcers and xerosis of the skin. Attached Figure Description

[0018] Figure 1 Statistical chart of moisturizing experiment results. Detailed Implementation

[0019] To enable those skilled in the art to better understand the technical content of this invention, the invention will be further described below with reference to specific embodiments.

[0020] Example 1

[0021] A method for preparing liquid functional dressings includes the following steps:

[0022] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve to obtain anise powder;

[0023] S2: Mix corn oil and anise powder obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0024] Example 2

[0025] A method for preparing liquid functional dressings includes the following steps:

[0026] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 50℃ and 25 kHz for 4 hours, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, adjust the pH to 8.5±0.2 with sodium hydroxide, sonicate at 50℃ and 25 kHz for 4 hours, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0027] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0028] Example 3

[0029] A method for preparing liquid functional dressings includes the following steps:

[0030] S1: Anise pretreatment: Crush the anise and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg: 10 mL, place it under ultrasonic treatment at 50℃ and 25 kHz for 4 h, filter, take the filtrate, concentrate to remove the solvent, and obtain anise extract concentrate.

[0031] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0032] Example 4

[0033] A method for preparing liquid functional dressings includes the following steps:

[0034] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 50℃ and 25 kHz for 4 hours, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, without adjusting the pH, sonicate at 50℃ and 25 kHz for 4 hours, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0035] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0036] Example 5

[0037] A method for preparing liquid functional dressings includes the following steps:

[0038] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 50℃ and 25 kHz for 3 h, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, adjust the pH to 8.5±0.2 with sodium hydroxide, sonicate at 50℃ and 25 kHz for 3 h, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0039] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0040] Example 6

[0041] A method for preparing liquid functional dressings includes the following steps:

[0042] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 55℃ and 25 kHz for 4 hours, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, adjust the pH to 8.5±0.2 with sodium hydroxide, sonicate at 60℃ and 25 kHz for 4 hours, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0043] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0044] Example 7

[0045] A method for preparing liquid functional dressings includes the following steps:

[0046] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 35℃ and 25 kHz for 4 h, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, adjust the pH to 8.5±0.2 with sodium hydroxide, sonicate at 50℃ and 25 kHz for 4 h, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0047] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0048] Example 8

[0049] A method for preparing liquid functional dressings includes the following steps:

[0050] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:20 mL, sonicate at 50℃ and 25 kHz for 4 h, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:20 mL, adjust the pH to 8.5±0.2 with sodium hydroxide, sonicate at 50℃ and 25 kHz for 4 h, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0051] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0052] Example 9

[0053] A method for preparing liquid functional dressings includes the following steps:

[0054] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 50℃ and 25 kHz for 4 hours, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, adjust the pH to 8.5±0.2 with sodium hydroxide, sonicate at 50℃ and 25 kHz for 4 hours, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0055] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:20 to obtain a liquid functional dressing.

[0056] Example 10

[0057] A method for preparing liquid functional dressings includes the following steps:

[0058] S1: Anise pretreatment: Grind the anise into powder and pass it through a 60-mesh sieve; mix the sieved anise powder with petroleum ether at a ratio of 1 mg:10 mL, sonicate at 50℃ and 25 kHz for 4 h, filter, and collect the filtrate; concentrate the filtrate to remove the solvent, and obtain the concentrate; mix the concentrate with 80 v / v% acetone aqueous solution at a ratio of 1 mg:10 mL, adjust the pH to 7.6±0.2 with sodium hydroxide, sonicate at 50℃ and 25 kHz for 4 h, filter, collect the filtrate, concentrate to remove the solvent, and obtain the anise extract concentrate;

[0059] S2: Mix corn oil and anise extract concentrate obtained in step S1 at a mass ratio of 70:30 to obtain a liquid functional dressing.

[0060] I. Scratch Test

[0061] The scratch test was used to determine whether each product promoted the migration of keratinocytes (HaCat).

[0062] (1) Take cells in the logarithmic growth phase (10 6 (cells / mL), seed 500 μL of cell solution into each well of the cell plate, incubate for 24 h, make a mark along the middle of the back of the culture well with the tip of a pipette, and wash the cells with PBS;

[0063] (2) Add 500 μL of drug-containing serum-free DMEM culture medium (drug content 20 μL / mL) to each well, and set up a negative control group (group 5); Note: The products used in each drug-added group are samples from Examples 1 to 4 (i.e., groups 1 to 4).

[0064] (3) After culturing the cell plate in an incubator for 12 hours, remove it, observe it under a microscope, take pictures and measure the scratch area. Calculate the healing rate by measuring the scratch area before and after culture. Repeat the experiment 3 times for each group.

[0065] Experimental results:

[0066] The migration of keratinocytes can reflect the wound healing process. The experimental results are shown in Table 1. The results showed that there were significant differences in the scratch healing rate among the drug-treated groups and between the drug-treated groups and the negative control group (p<0.05), indicating that the drug-treated groups had a significant promoting effect on the migration of HaCat cells.

[0067] Further comparison of the scratch healing rates among the different treatment groups revealed that the sample in Example 1 (a liquid dressing made by mixing corn oil and anise powder) also exhibited a certain wound repair ability, which may be related to the linoleic acid, linolenic acid, and vitamin E components in corn oil. The sample in Example 2 underwent pretreatment of anise, firstly by ultrasonic extraction at 50°C, and then by extraction of its alkali-soluble active ingredients in an acetone aqueous solution at 50°C. The product formed by mixing these alkali-soluble active ingredients with corn oil showed better wound repair ability, with a scratch healing rate reaching 63%. Compared to Example 2, the sample in Example 3 did not further extract the alkali-soluble active ingredients in an acetone aqueous solution at 50°C, resulting in a significantly lower scratch healing rate. Compared to Example 2, Example 4 did not adjust the pH, resulting in a significantly lower scratch healing rate than Example 2, indicating that alkaline conditions during the extraction process play a crucial role.

[0068] Table 1. Results of Analysis of Variance

[0069]

[0070] II. Tube Formation Experiment

[0071] The effect of angiogenesis was quantified through tubule formation experiments. The number of newly formed blood vessels reflects, to some extent, the speed of wound healing.

[0072] (1) Spread 200 μL of matrix gel in each well of the cell plate and fix overnight;

[0073] (2) Resuspend human umbilical vein endothelial cells (HUVECs) in logarithmic growth phase in a drug-treated medium containing 10 μL / mL of the drug to achieve a cell concentration of 10. 4 cell / mL;

[0074] (3) Add 1 mL of the drug-containing cell solution from step (2) to the cell plate coated with matrix gel. A negative control was also set up. Note: The products used in each drug-treated group were samples from Examples 1-4 (i.e., groups 1-4).

[0075] (4) After 8 hours of culture, observe and photograph under a microscope, count the number of nodes and the length of tubes, and calculate the relative number of nodes (number of nodes in the drug group / number of nodes in the negative control group × 100%) and the relative length of tubes (length of tubes in the drug group / length of tubes in the negative control group × 100%).

[0076] Experimental results:

[0077] The results are shown in Tables 2 and 3. The results showed that the relative number of nodes in groups 2–4 was significantly higher than that in group 1, and the relative number of nodes in group 2 was significantly higher than that in groups 3 and 4. The relative tubule length in groups 2–4 was higher than that in group 1, and the relative tubule length in group 2 was significantly higher than that in groups 3 and 4. Overall, the results indicate that sample group 2 had the best ability to promote angiogenesis.

[0078] Table 2 Results of Analysis of Variance

[0079]

[0080] Table 3. Results of Analysis of Variance

[0081]

[0082] III. Moisturizing Experiment

[0083] Weigh 1.0g of sample 2 (Group 1) or 1.0g of glycerol (Group 2) and place it in a weighing bottle. Add an equal mass of deionized water and place the bottle open in a desiccator containing silica gel. Place the bottle for 5, 10, 15, 20, and 25 hours, respectively, and weigh the total mass of the sample before and after each placement. Repeat the experiment three times and calculate the average value.

[0084] Moisture retention rate = (H1 / H0) × 100%. Where, H1: total mass of the sample after placement; H0: initial total mass of the sample (sample + water + bottle).

[0085] Experimental results:

[0086] See Figure 1 The results showed that, compared with the glycerin group, the product of this invention had better moisturizing properties and exhibited a certain degree of long-lasting effect. After 10 hours of testing, the moisturizing rate was significantly higher than that of the glycerin group.

[0087] The products from Examples 5 to 10 were used in Experiments 1 to 3 above, and the results were similar to those of the sample from Example 2, with no significant difference.

[0088] The above description is only a part of the embodiments of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention shall fall within the protection scope of the present invention.

Claims

1. A method for preparing a liquid functional dressing for preventing pressure ulcers, moisturizing skin, and repairing skin wounds, characterized by, The method comprises the following steps: S1: Anise pretreatment: crushing anise, sieving, obtaining anise powder; mixing the anise powder with petroleum ether, ultrasonic treatment under the condition of 35-55℃, filtering, taking the filtrate; concentrating the filtrate to remove the solvent, obtaining a concentrate, mixing the concentrate with an aqueous acetone solution and adjusting the pH to be alkaline, ultrasonic treatment under the condition of 40-60℃, filtering, taking the filtrate, concentrating to remove the solvent, obtaining an anise extract concentrate; S2: mixing corn oil with the anise extract concentrate obtained in step S1, obtaining a liquid functional dressing.

2. The production method according to claim 1, characterized by, In step S2, the mass ratio of corn oil to anise extract concentrate is 70:20-30.

3. The preparation method according to claim 1, characterized in that, The alkalinity is pH 7.6-8.

7.

4. The production method according to claim 1, characterized by, The concentration of the aqueous acetone solution is 80v / v%-90v / v%.

5. The preparation method according to claim 1, characterized in that, The ultrasonic treatment time is 3-4h.

6. The method of claim 1, wherein, In step S1, the solid-liquid ratio of anise powder to petroleum ether is 1mg:10-20mL.

7. The preparation method according to claim 1, characterized in that, In step S1, the solid-liquid ratio of the concentrate to the aqueous acetone solution is 1mg:10-20mL.

8. The liquid functional dressing prepared by the preparation method of claim 1.

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