A grafting seedling raising method for agarwood with high survival rate and easy to form fragrance

Abstract

The present invention provides a grafting seedling raising method for agarwood with high survival rate and easy to form fragrance, which belongs to the technical field of agarwood seedling raising. The grafting seedling raising method for agarwood with easy to form fragrance of the present invention comprises the following steps: (1) preparing agarwood rootstock; (2) 8 to 12 days before collecting scions, cutting off the 20 to 30 cm portion below the top of the agarwood branch to promote the germination of latent buds of the scion branch, and collecting the scion from the branch with bud eyes; (3) grafting the agarwood rootstock and the scion, and bagging the grafted scion and the grafting site to keep them moist; (4) covering the ground at the root of the grafted rootstock with formulated soil to harden the seedling. Compared with traditional methods, the grafting seedling raising method of the present invention can significantly improve the survival rate, number of buds and length of buds of grafted seedlings, and provide a new guiding idea for breeding excellent strains of agarwood with easy to form fragrance.

Description

Technical Field

[0001] The invention relates to the technical field of agarwood seedling cultivation, and in particular to a grafting seedling cultivation method for agarwood with high survival rate and easy to produce fragrance. Background Art

[0002] Aquilaria sinensis (Lour.) Gilg, as a precious spice and Chinese medicinal material, has now become a new favorite in the collection market and is known as the "diamond among plants". In recent years, due to the scarcity of resources, the price of agarwood has continued to rise by more than 30% for two consecutive years. Most native agarwood trees have a long fragrance production cycle and low yield, but in the natural agarwood forest, it is found that there are also 5% to 7% of excellent variant plants that can naturally produce agarwood, which are excellent individuals of agarwood that is easy to produce agarwood. Through screening in the past decade, some agarwood strains that are easy to produce agarwood, such as watermelon leaves, golden silk oil and oil leaves, have been selected. They have the excellent characteristics of fast natural agarwood production, high yield and high fragrance quality, and the production cycle is shortened to 6 to 10 years. However, the excellent characteristics of these easy-to-produce agarwood strains are easily lost through sexual reproduction, and can only be preserved through asexual reproduction. Agarwood can be propagated by tissue culture or cuttings, but people try to use the same method to propagate agarwood seedlings of excellent strains that are easy to produce fragrance, but the survival rate is low and it is very difficult. How to propagate asexual seedlings is related to the development of the agarwood industry. Therefore, it is necessary to provide a method for grafting agarwood seedlings with a high survival rate and easy to produce fragrance. Summary of the invention

[0003] The purpose of the present invention is to provide a grafting seedling raising method for agarwood with high survival rate. Compared with the traditional method, the grafting seedling raising method of the present invention can significantly improve the survival rate, number of buds and length of buds of grafted seedlings, and provide a new guiding idea for breeding excellent strains of agarwood with high survival rate.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The present invention provides a method for grafting and raising seedlings of agarwood with high survival rate, comprising the following steps:

[0006] (1) Prepare agarwood rootstock;

[0007] (2) 8 to 12 days before collecting the scion, cut off the 20 to 30 cm below the top of the agarwood branch to promote the germination of the latent buds of the scion branch, and collect the scion from the branch with buds;

[0008] (3) grafting the agarwood rootstock and the scion, and bagging the grafted scion and the grafting site to keep them moist;

[0009] (4) Covering the root of the grafted stock with formulated soil to harden the seedlings.

[0010] Preferably, the agarwood rootstock is 12 to 18 months old.

[0011] Preferably, the roots of the agarwood rootstock are thoroughly watered 4 to 7 days before grafting.

[0012] Preferably, the rootstock has a ground diameter of 1.00 to 1.99 cm and a height of 0.6 to 1.0 m.

[0013] Preferably, the base diameter of the scion is 0.50 to 0.89 cm, the length is 6 to 10 cm, the scion has 2 to 3 leaves, and the leaves are cut off by 1 / 2 to 3 / 4 of the area.

[0014] Preferably, the grafting time is early March or mid-to-late October each year.

[0015] Preferably, the grafting is a cut grafting method.

[0016] Preferably, the formula soil is a mixture of red soil and river sand, and the mass ratio of the red soil to the river sand is (7-9):(1-3); the covering thickness of the formula soil is 2-4 cm; the timing of covering the formula soil is the day after the grafting in step (2) or the next day.

[0017] Preferably, during the hardening period, no watering is performed within the first 0 to 30 days; on the 18th to 22nd day, fungicide A is sprayed; on the 30th to 40th day, the bag at the grafting site is removed, and fungicide B and insecticide are sprayed; thereafter, fungicide B and insecticide are sprayed every 12 to 18 days.

[0018] Preferably, during the seedling hardening period, shading is performed on the 0th to 100th day, with a shading rate of 60 to 75%, and then the seedlings are hardened in full light.

[0019] The present invention provides a method for grafting and raising seedlings of agarwood with high survival rate. In the process of agarwood grafting, the present invention performs a pretreatment of cutting the top to promote budding before collecting the scion. By cutting the top to promote budding for a period of time, latent buds are promoted to germinate, the bud eyes are plump, the growth state of the branches is stronger, and the growth ability of the obtained scion is more vigorous, thereby improving the survival rate of the grafted seedlings. The present invention adjusts the overall growth state of the scion by adjusting the base diameter and leaves of the scion to improve the survival rate and bud length after grafting. The present invention also adopts a bagging method for moisturizing, and all scions and part of the stock are covered and sealed with a transparent plastic bag. Compared with the traditional binding method, the moisturizing effect is stronger and the survival rate of the grafted seedlings can be improved; after the grafting is completed, the stock is covered with a formula soil mixed with red soil and river sand, and the soil where the stock is planted is moisturized, which also has the effect of improving the survival rate of the grafted seedlings.

[0020] By adopting the grafting seedling raising method of the present invention, the average survival rate of the agarwood seedlings 180 days after grafting can reach 95%, the number of sprout branches can reach 2.7 branches / plant, and the sprout length can reach more than 23 cm. When the grafted seedlings in mid-to-late October grow to mid-to-late May of the following year, the average seedling height can reach more than 40 cm, and they can be planted in the nursery. The seedlings are of moderate size, convenient for transportation and planting, and this period is in the rainy season, so the survival rate of afforestation is very high, and the young trees grow fast after planting. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is the growth state of the agarwood branches after top pruning to promote budding in Example 1 and before scion pruning.

[0022] Figure 2 This is the growth state of the agarwood branches after top pruning to promote budding in Example 1 and before scion pruning.

[0023] Figure 3 This is the process of treating the scion of Example 1.

[0024] Figure 4 This is the state of bagging and moisturizing after the grafting is completed in Example 1.

[0025] Figure 5 The state of the stock covered with soil after the grafting is completed in Example 1.

[0026] Figure 6 The state of the stock covered with soil after the grafting is completed in Example 1. DETAILED DESCRIPTION

[0027] The present invention provides a method for grafting and raising seedlings of agarwood with high survival rate, comprising the following steps:

[0028] (1) Prepare agarwood rootstock;

[0029] (2) 8 to 12 days before collecting the scion, cut off the 20 to 30 cm below the top of the agarwood branch to promote the germination of the latent buds of the scion branch, and collect the scion from the branch with buds;

[0030] (3) grafting the agarwood rootstock and the scion, and bagging the grafted scion and the grafting site to keep them moist;

[0031] (4) Covering the root of the grafted stock with formulated soil to harden the seedlings.

[0032] The present invention prepares agarwood rootstock.

[0033] In the present invention, the roots of the agarwood rootstock are preferably watered thoroughly 4 to 7 days before grafting; more preferably, the roots are watered thoroughly 5 days before grafting.

[0034] In the present invention, the age of the agarwood rootstock is preferably 12 to 18 months, more preferably 15 months.

[0035] In the present invention, the ground diameter of the rootstock is preferably 1.00 to 1.99 cm, and more preferably 1.50 cm.

[0036] In the present invention, the height of the rootstock is preferably 0.6 to 1.0 m, and more preferably 0.8 m.

[0037] The invention cuts off the 20-30 cm part below the top of the agarwood branch 8-12 days before the scion is collected, so as to promote the germination of latent buds of the scion branch, and collects the scion from the branch with the germinated buds.

[0038] In the present invention, it is preferred that 8 to 12 days before the scion is collected, the portion 20 to 30 cm below the top of the agarwood branch is cut off to promote the germination of latent buds of the scion branch, and the scion is collected from the branch with germinated buds; it is further preferred that 10 days before the scion is collected, the portion 20 to 30 cm below the top of the agarwood branch is cut off to promote the germination of latent buds of the scion branch, and the scion is collected from the branch with germinated buds; it is further preferred that 10 days before the scion is collected, the portion 25 cm below the top of the agarwood branch is cut off to promote the germination of latent buds of the scion branch, and the scion is collected from the branch with germinated buds.

[0039] In the present invention, the base diameter of the scion is preferably 0.50 to 0.89 cm, and more preferably 0.80 cm.

[0040] In the present invention, the length of the scion is preferably 6 to 10 cm, more preferably 8 cm.

[0041] In the present invention, the scion preferably has 2 to 3 leaves, and more preferably has 2 leaves.

[0042] In the present invention, the blade is preferably cut off by 1 / 2 to 3 / 4 of its area, and more preferably cut off by 3 / 4 of its area.

[0043] The invention grafts the agarwood rootstock and the scion, and bags the grafted scion and the grafted part to keep them moist.

[0044] In the present invention, the grafting time is preferably early March or mid-to-late October each year, and more preferably mid-to-late October each year.

[0045] In the present invention, the grafting is preferably a cut grafting method.

[0046] In the present invention, the bagging for moisture retention is preferably to use a transparent plastic bag with a sealing pressure line to cover the grafted scion and the grafting part, and to press the sealing pressure line tightly.

[0047] The invention covers the ground at the root of the grafted stock with formulated soil to harden the seedlings.

[0048] In the present invention, the formula soil is preferably a mixture of red soil and river sand.

[0049] In the present invention, the mass ratio of the red soil to the river sand is preferably (7-9):(1-3), and more preferably 8:2.

[0050] In the present invention, the covering thickness of the formulated soil is preferably 2 to 4 cm, more preferably 3 cm.

[0051] In the present invention, the timing of covering with the formulated soil is preferably on the day after grafting or the next day, and more preferably on the day after grafting.

[0052] In the present invention, during the hardening period, preferably within the 0th to 30th days, no watering is performed.

[0053] In the present invention, during the hardening period, fungicide A is preferably sprayed on the 18th to 22nd day; more preferably, fungicide A is sprayed on the 20th day.

[0054] In the present invention, the fungicide A is preferably 800 to 1200 times diluted Biotite, and more preferably 1000 times diluted Biotite.

[0055] In the present invention, during the hardening period, preferably on the 30th to 40th day, the bag at the grafting site is removed, and fungicide B and insecticide are sprayed, and then the fungicide B and insecticide are sprayed every 12 to 18 days; more preferably, on the 35th day, the bag at the grafting site is removed, and fungicide B and insecticide are sprayed, and then the fungicide B and insecticide are sprayed every 15 days.

[0056] In the present invention, the fungicide B is preferably a 1200-1500 times solution of Biotide or a 1200-1500 times solution of Ridomil, more preferably a 1400 times solution of Biotide or a 1400 times solution of Ridomil, and even more preferably a 1400 times solution of Ridomil.

[0057] In the present invention, the insecticide is preferably a 1000-1200 times dilution of cypermethrin, and more preferably a 1100 times dilution of cypermethrin.

[0058] In the present invention, during the seedling hardening period, preferably, shading is performed on the 0th to 100th day with a shading rate of 60-75%, and then the seedlings are hardened in full light; more preferably, shading is performed on the 0th to 100th day with a shading rate of 70%, and then the seedlings are hardened in full light.

[0059] In the present invention, during the hardening of the seedlings until March of the following year, preferably, the compound fertilizer aqueous solution is applied once every 18 to 25 days; more preferably, the compound fertilizer aqueous solution is applied once every 20 days.

[0060] In the present invention, the mass concentration of the compound fertilizer aqueous solution is preferably 0.2-0.3%, and more preferably 0.25%.

[0061] In the present invention, the compound fertilizer in the compound fertilizer aqueous solution is preferably NPK: 15-15-15.

[0062] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0063] Example 1

[0064] This embodiment provides a method for grafting and raising seedlings of agarwood, and the specific process is as follows:

[0065] Location: Tianma State Forest Farm Nursery, Pinghe, Fujian Province. The grafting experiment was conducted in a simple greenhouse built with galvanized pipes. The top of the greenhouse arch was covered with film and sunshade nets, and automatic spray water pipe facilities were installed in the greenhouse. The ground was leveled so that the nutrient cup rootstock could be directly placed on the ground for cultivation.

[0066] 1. Rootstock cultivation

[0067] The present embodiment selects the white wood fragrant variety to cultivate stock. This variety blooms in late March to mid-April in the coastal areas of Zhangzhou, and the fruit matures in mid-July of that year. Select the white wood fragrant superior trees of more than 6 years old to collect seeds. After the seeds are harvested and shelled, they are directly taken for sowing within 2 days, and the germination rate is higher (if they cannot be sown immediately, the seeds should be mixed with fine sand with a moisture content of 45% humidity and then bagged and stored in a cool place. Sowing is completed within 1 week. If the storage time exceeds 2 months, it almost loses the ability to germinate). After soaking the seeds in 35 ℃ of warm water for 12 hours, the seeds are directly sown on the sand bed, and the thickness of the fine sand covering is about 1cm, so that the seeds are not visible. The seedlings like shade. After sowing, 70% sunshade nets are covered on the top of the arch shed, and the seedbed is sprinkled with water to keep the moisture, and it starts to germinate 10 days after sowing. The seedlings with a sprout height of 5 cm were transplanted into plastic nutrient cups with a size of 14 cm × 14 cm. The nutrient soil in the cups was a formula soil in which red soil and river sand were evenly mixed in a mass ratio of 7:3. The seedlings were cultivated into rootstocks with a seedling age of 15 months.

[0068] 2. Pretreatment of rootstock and branches

[0069] Select a rootstock with a ground diameter of 1.5 cm and a height of 0.8 m. Five days before grafting, water the soil in the rootstock nutrient bag thoroughly with clean water.

[0070] 10 days before grafting, the scion branches are pre-treated by cutting off the top 25cm long part of the watermelon leaf branches of the agarwood easy-to-bear fragrance variety. After cutting off the top to promote budding, the hidden buds of the branches are germinated and the buds are full (such as Figures 1-2As shown in the figure, the growth state of the agarwood branches after pruning to promote budding and before cutting the scion). Before grafting, select branches with a base diameter of 0.80 cm and cut them into scions with a length of 8 cm. Each scion has 2 leaves, and 3 / 4 of the leaf area is cut off from each leaf (the scion processing process is as shown in the figure). Figure 3 The scion is grafted as soon as it is collected.

[0071] 3. Rootstock and scion grafting

[0072] On October 22, 2022, the rootstock and scion were grafted by the cut grafting method. After the connection between the rootstock and the scion was tied with a plastic tape, all the scions and most of the rootstock were covered with a transparent plastic bag with a length of 15 cm, a width of 9 cm, and a thickness of 0.16 mm and a sealing pressure line, and the sealing strip (such as Figure 4 shown).

[0073] On the day of grafting, the formula soil obtained by mixing 80% red soil + 20% river sand is evenly covered on the rootstock nutrient bag with a covering thickness of 3 cm (such as Figures 5-6 shown).

[0074] 4. Hardening the seedlings

[0075] After grafting, do not water within 30 days. Spray 1000 times of Baitai solution once on the 20th day after grafting; on the 30th day, when the length of the scion sprout reaches 3cm, take off the plastic bag and spray 1400 times of Ridomil solution + 1100 times of Cypermethrin solution at the same time. Spray 1400 times of Ridomil solution + 1100 times of Cypermethrin solution every half a month. On the 100th day, remove the sunshade net of the greenhouse and carry out full light hardening.

[0076] After hardening the seedlings until March, water them with a 0.25% compound fertilizer (15% each of N, P, and K) aqueous solution every 20 days, and weed out the rootstock sprouts in time.

[0077] After the above grafting treatment and management, in October, when the grafted seedlings grew to the 180th day, the average survival rate reached more than 95%, the number of budding branches reached 2.7 branches / plant, and the length of budding reached more than 23cm; in mid-to-late May of the following year, the average seedling height was more than 40cm, and it was ready for planting. The seedlings were of moderate size and convenient for transportation and planting. 15 days before planting, the roots were removed to further harden the seedlings and improve the survival rate of afforestation.

[0078] Comparative Example 1

[0079] This comparative study studied the effect of rootstock diameter on the growth of grafted agarwood seedlings. The specific process is as follows:

[0080] Due to the differences in growth between cultivated agarwood individuals, in order to select more test rootstocks, this comparative example grouped the rootstocks according to the ground diameter range of 1.50-1.99 cm, 1.00-1.49 cm, and 0.50-0.99 cm when selecting the rootstocks. The selected rootstocks were from the rootstocks cultivated in step 1 of Example 1, and the rootstock height was 0.8 m.

[0081] The other processes were the same as those in Example 1. 100 days after the grafting of agarwood, the survival rate and growth of the grafted seedlings of rootstocks with different ground diameters were statistically analyzed, and the statistical results are shown in Table 1.

[0082] Table 1 Survival rate and growth of agarwood grafted with different diameter rootstocks

[0083] Rootstock diameter / cm Survival rate / % Number of budding branches Bud length / cm 1.50～1.99 98.20 3.17 11.34 1.00～1.49 96.77 2.70 10.61 0.50～0.99 74.97 2.17 10.50

[0084] As can be seen from Table 1, the grafting survival rate and growth of different rootstock diameters vary greatly. Within the reasonable diameter range, the larger the rootstock, the stronger the rootstock, the higher the grafting survival rate, and the better the budding and shoot growth. The average survival rate of grafting rootstocks with a root diameter between 1.00cm and 1.99cm is 97.5%, which is 22.53% higher than the survival rate of rootstocks with a root diameter below 1.00cm.

[0085] Comparative Example 2

[0086] This comparative example studies the effect of agarwood branch pretreatment on the growth of agarwood grafted seedlings. The specific process is as follows:

[0087] According to the different pretreatment conditions of the agarwood branches, two groups were set up, namely, a group treated with pruning and bud promotion and a group treated without pruning and bud promotion. The pruning and bud promotion group was operated according to Example 1 to obtain scions; the group treated without pruning and bud promotion was directly pruning branches that had not been treated with pruning and bud promotion as scions for grafting.

[0088] The rootstock selected was the rootstock cultivated in step 1 of Example 1, with a ground diameter of 1.50 cm (variable range of 1.00 cm to 1.99 cm) and a height of 0.8 m. The other processes were the same as in Example 1. Six months after the grafting of agarwood, the survival rate and growth of the grafted seedlings with different pretreatment methods were statistically analyzed, and the statistical results are shown in Table 2.

[0089] Table 2 Survival rate and growth of agarwood grafting with different pretreatment methods

[0090] Scion treatment Survival rate / % Number of budding branches Bud length / cm Cutting the top to promote budding 96.53 2.64 24.31 No pruning to promote budding 85.97 2.47 23.38

[0091] As can be seen from Table 2, the survival rate of grafting with scions that have been top-cut to promote budding can reach 96.53%, which is 10.56% higher than that of grafting with scions that have not been top-cut to promote budding. And after top-cutting to promote budding, the budding time can be advanced by 5 to 7 days. There are many hidden buds hidden in the branches of agarwood. Top-cutting to promote budding 10 days before collecting scions can promote the germination of hidden buds in scions, and the buds are full, the survival rate of scions is higher, and the growth is better.

[0092] Comparative Example 3

[0093] This comparative example studies the effect of scion treatment methods on the growth of grafted agarwood seedlings. The specific process is as follows:

[0094] According to the different scion treatment methods, 7 groups were set up, and the specific treatment methods of each group are shown in Table 3 (due to the differences in growth conditions between agarwood individuals, in order to select more test scions, when selecting scions for this comparative example, the scions were grouped according to the base diameter ranges of 0.70-0.89 cm, 0.50-0.69 cm, and 0.30-0.49 cm).

[0095] The rootstock selected was the rootstock cultivated in step 1 of Example 1, with a ground diameter of 1.50 cm (variable range of 1.00 cm to 1.99 cm) and a height of 0.8 m. The other processes were the same as in Example 1. 100 days after the grafting of the agarwood, the survival rate and growth of the grafted seedlings with different scion treatments were statistically analyzed, and the statistical results are shown in Table 3.

[0096] Table 3 Survival rate and growth of agarwood grafts with different scion treatment methods

[0097]

[0098]

[0099] As can be seen from Table 3, the grafting survival rate and new shoot growth of scion with a base diameter of 0.70cm to 0.89cm, 2 leaves, and 3 / 4 of the area cut off are the best, reaching 98.3% and 12.61cm respectively. The base diameter of the scion directly affects the grafting survival rate and new shoot growth, but has no obvious effect on the number of buds from the scion. Generally speaking, the scion is thicker and stores more nutrients and water in the body, which is not easy to disappear in a short time, and the grafting effect is better. Grafting of scion with leaves and without leaves (traditional method) has a great influence on the survival rate, the number of buds and the length of buds.

[0100] Comparative Example 4

[0101] This comparative example studies the effect of grafting methods on the growth of grafted agarwood seedlings. The specific process is as follows:

[0102] According to different grafting methods, two groups were set up, one group was the method of Example 1 of the present invention (all operations were the same as those of Example 1), and the other group was the traditional method without top pruning to promote budding and without bagging to keep moisture. The scion collection process without top pruning to promote budding was the same as that of the group without top pruning to promote budding in Comparative Example 2.

[0103] The rootstock selected was the rootstock cultivated in step 1 of Example 1, with a ground diameter of 1.50 cm (variable range of 1.00 cm to 1.99 cm) and a height of 0.80 m. The other processes were the same as in Example 1. Six months after the grafting of the agarwood, the survival rate and growth of the grafted seedlings of different grafting methods were statistically analyzed, and the statistical results are shown in Table 4.

[0104] Table 4 Survival rate and growth of agarwood grafted by different methods

[0105]

[0106] It can be seen from Table 4 that the grafting method of the present invention can improve the survival rate by 36.61% compared with the traditional method. Bag moisture retention can prevent the scion from losing water, so that the scion can be grafted with leaves, which can improve the grafting survival rate.

[0107] Comparative Example 5

[0108] This comparative example studies the effect of rootstock soil covering on the growth of grafted agarwood seedlings. The specific process is as follows:

[0109] According to different rootstock covering operations, two groups were set up, one group was the method of Example 1 of the present invention (all operations were the same as Example 1), and the other group was the traditional method without covering.

[0110] The rootstock selected was the rootstock cultivated in step 1 of Example 1, with a ground diameter of 1.50 cm (variable range of 1.00 cm to 1.99 cm) and a height of 0.80 m. The other processes were the same as in Example 1. 100 days after the grafting of the agarwood, the survival rate and growth of the grafted seedlings with different rootstocks covered with soil were statistically analyzed, and the statistical results are shown in Table 5.

[0111] Table 5 Survival rate and growth of agarwood grafted with different rootstocks covered with soil

[0112]

[0113] As can be seen from Table 5, the formula soil mixed with 80% red soil + 20% river sand is evenly covered on the rootstock nutrient bag with a thickness of 3cm, which can achieve a good moisturizing effect and significantly improve the grafting survival rate, which is 10.37% higher than that without covering soil, but has no obvious effect on the number of sprout branches and the length of sprouts. After the grafting of agarwood, the scion has not yet healed in a short time, and no new shoots have sprouted and cannot perform photosynthesis. The soil cannot be too wet. Therefore, in order to improve the grafting survival rate, the agarwood cannot be watered 3d before grafting and about 30d after grafting. However, the soil in the nutrient bag cannot be too dry and needs to maintain appropriate humidity. At this time, the formula soil of the present invention is used to cover the nutrient bag, which can maintain soil moisture and improve the survival rate.

[0114] The present invention has also conducted research on ordinary soil covering, but the effect is poor; covering with pure red soil is easy to harden and has poor water permeability after watering or rainfall; covering with pure river sand has poor water retention.

[0115] Comparative Example 6

[0116] This comparative study studied the effect of grafting time on the growth of grafted agarwood seedlings. The specific process is as follows:

[0117] According to the different grafting times, 4 groups are set up, namely February 10, 2022, March 20, 2022, September 20, 2022, and October 20, 2022.

[0118] The rootstock selected was the rootstock cultivated in step 1 of Example 1, with a ground diameter of 1.50 cm (variable range of 1.00 cm to 1.99 cm) and a height of 0.8 m. The other processes were the same as in Example 1. Six months after the grafting of the agarwood, the survival rate and growth of the grafted seedlings at different grafting times were statistically analyzed, and the statistical results are shown in Table 6.

[0119] Table 6 Survival rate and growth of agarwood grafted at different grafting times

[0120] Grafting time Survival rate / % Number of budding branches Bud length / cm February 10, 2022 86.10 2.36 23.43 March 1, 2022 93.97 2.44 28.22 September 20, 2022 59.90 2.17 16.03 October 20, 2022 95.83 2.70 23.22

[0121] As can be seen from Table 6, in Zhangzhou area, grafting around March 1 and October 20 is better than grafting at other times. The highest survival rate can reach 95.83% after 6 months of grafting, the number of budding branches is 2.70 per plant, and the length of budding is 28.22cm. However, the budding period of spring shoots of Aquilaria sinensis is in early March. The weather in spring is changeable and there is a lot of rain. If it is rainy, grafting cannot be done and the scion will sprout spring shoots, which is easy to miss the best grafting period. On October 20, the weather enters the autumn season, the grafting period is long, it is easy to carry out grafting work, and the grafting survival rate is high, so it is more appropriate to choose the time for large-scale grafting in mid-to-late October.

[0122] Comparative Example 7

[0123] This comparative study studied the effect of seedling hardening management methods on the growth of grafted agarwood seedlings. The specific process is as follows:

[0124] According to different seedling hardening management methods, three groups were set up: T1 was for hardening the seedlings until the 100th day, the shade net was lifted; T2 was for hardening the seedlings until the 70th day, and T3 was for not lifting the shade net.

[0125] The rootstock selected was the rootstock cultivated in step 1 of Example 1, with a ground diameter of 1.50 cm (variable range of 1.00 cm to 1.99 cm) and a height of 0.8 m. The other processes were the same as in Example 1. Six months after the grafting of agarwood, the survival rate and growth of the grafted seedlings with different seedling hardening management methods were statistically analyzed, and the statistical results are shown in Table 7.

[0126] Table 7 Survival rate and growth of agarwood grafting under different seedling hardening management methods

[0127]

[0128]

[0129] As can be seen from Table 7, in general, it is most suitable to remove the shade net and harden the seedlings in full light 100 days after grafting. Compared with raising seedlings with shade nets throughout the whole process, the sprout length increased by 7.9 cm, the growth rate was 36.7%, and the number of sprout branches increased by 7.6%. The growth of seedlings in full light was better than that at an earlier time, but it had no obvious effect on the survival rate.

[0130] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for raising seedlings of agarwood grafted with a high survival rate, characterized in that: The steps include: (1) preparing agarwood rootstock; the rootstock has a ground diameter of 1.00-1.99 cm and a height of 0.6-1.0 m; (2) 8 to 12 days before collecting the scion, cut off the 20 to 30 cm part below the top of the agarwood branch to promote the germination of the latent buds of the scion branch, and collect the scion from the branch with buds; (3) grafting the agarwood rootstock and the scion, and bagging the grafted scion and the grafted joint to keep them moist; (4) Covering the ground around the root of the grafted stock with formulated soil to harden the seedlings until March of the following year, and then applying a compound fertilizer solution every 18 to 25 days until the seedlings are ready for transplanting; The roots of the agarwood rootstock are watered thoroughly 4 to 7 days before grafting; The base diameter of the scion is 0.50-0.89 cm, the length is 6-10 cm, the scion has 2-3 leaves, and the leaves are cut off by 1 / 2-3 / 4 of the area; The formula soil is a mixture of red soil and river sand, and the mass ratio of the red soil to the river sand is (7-9): (1-3); the covering thickness of the formula soil is 2-4 cm; the timing of covering the formula soil is the day after the grafting or the next day; During the hardening period, no watering is done within the first 0 to 30 days; on the 18th to 22nd day, fungicide A is sprayed; on the 30th to 40th day, the bag at the grafting site is removed, and fungicide B and insecticide are sprayed, and then fungicide B and insecticide are sprayed every 12 to 18 days; The bactericide A is 800-1200 times diluted Baitai; The fungicide B is a 1200-1500 times solution of Biotide or a 1200-1500 times solution of Ridomil; The insecticide is 1000-1200 times diluted cypermethrin; During the hardening period, shading is performed on the 0th to 100th day, with a shading rate of 60-75%, and then the seedlings are hardened in full light; The mass concentration of the compound fertilizer aqueous solution is 0.2-0.3%; The compound fertilizer in the compound fertilizer aqueous solution is NPK: 15-15-15.

2. The grafting seedling raising method according to claim 1, characterized in that: The seedling age of the agarwood rootstock is 12 to 18 months.

3. The grafting seedling raising method according to claim 2, characterized in that: The time of the grafting is early March or mid to late October each year.

4. The grafting seedling raising method according to claim 3, characterized in that: The grafting is a cut grafting method.

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