Automatic decalcification and water flushing integrated machine

By designing an automatic decalcification and rinsing machine that integrates a lifting device and a swing mechanism, the problem of untimely replacement of decalcification solution in pathological experiments has been solved, achieving automated operation, improving experimental efficiency and reducing safety risks.

CN117929056BActive Publication Date: 2026-05-05FOURTH MILITARY MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FOURTH MILITARY MEDICAL UNIVERSITY
Filing Date
2024-02-03
Publication Date
2026-05-05

AI Technical Summary

Technical Problem

In existing technologies, the decalcification rinsing process in pathological experiments mainly relies on manual operation, which leads to untimely or forgotten replacement of the decalcification solution, affecting the experimental results. Furthermore, the experimenters' long-term contact with chemical reagents poses a safety hazard.

Method used

An automatic decalcification and rinsing integrated machine was designed, which includes a sample chamber, a decalcification tank and a shaking table. Combined with a lifting device, a decalcification liquid adding mechanism and a swinging mechanism, it realizes the integration of automated decalcification, rinsing and heating processes, reducing manual operation steps.

Benefits of technology

It improves experimental efficiency, saves laboratory space, reduces costs, and reduces the risk of experimenters coming into contact with chemical reagents through automated operation.

✦ Generated by Eureka AI based on patent content.

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Abstract

This invention provides an automatic decalcification and rinsing integrated machine, comprising a sample chamber, a washing tank, and a shaking table. A lifting device is slidably mounted on the washing tank and magnetically connected to the sample chamber. The upper part of the washing tank is a rinsing chamber, and the lower part is a decalcification chamber, separated by a horizontal partition. A movable cover is installed on the top of the sample chamber, and the lower four-fifths of the side wall of the sample chamber is a mesh plate. An automatic decalcification solution addition mechanism is connected to the decalcification chamber, and a heating ring is installed at the bottom of the decalcification chamber wall. An inlet and outlet are provided on the rinsing chamber. The washing tank is connected to the shaking table platform below. This invention has a scientifically sound and reasonable structural design, is simple to operate, convenient to use, and highly practical. It effectively combines decalcification and rinsing, and can be widely used.
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Description

Technical Field

[0001] This invention relates to a sample decalcification device, specifically to an automatic decalcification and rinsing integrated machine. Background Technology

[0002] Histopathological experiments are an important technique in biological and clinical medical research, providing invaluable information for the medical and life science fields. By preparing pathological tissue sections, observing the morphology and structure of tissues and cells, we can understand the occurrence and development of diseases, providing important references and bases for formulating pathological diagnosis and prevention plans. Currently, in most research institutions and other experimental settings, the decalcification and rinsing process before pathological experiments is still entirely done manually by the experimenter. This inevitably leads to untimely or forgotten replacement of the decalcification solution, resulting in decalcification exceeding the time limit and affecting the experimental results. In addition, although the experimenter wears medical gloves, prolonged contact with such chemical reagents can still cause certain harm. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide an automatic decalcification and rinsing integrated machine that addresses the shortcomings of the prior art. The device has a scientific and reasonable structural design, a high degree of integration, saves laboratory space, is easy to operate, improves experimental research efficiency, is low in cost, and can be widely used.

[0004] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: an automatic decalcification and rinsing integrated machine, characterized in that it includes a sample chamber, a washing tank, and a shaking table. A lifting device is slidably arranged on the washing tank, and the lifting device is magnetically connected to the sample chamber. The upper part of the washing tank is a rinsing chamber, and the lower part is a decalcification chamber. The decalcification chamber and the rinsing chamber are separated by a horizontal partition. A movable cover is provided on the top of the sample chamber. The lower four-fifths of the side wall of the sample chamber is a mesh plate. A shaking table is connected to the bottom of the decalcification chamber. A decalcification liquid adding mechanism is connected to the decalcification chamber. A heating ring is provided at the bottom of the decalcification chamber. An inlet and an outlet are provided on the rinsing chamber.

[0005] Preferably, the lifting device includes an operating lever, a slider, and an electromagnet. The washing tub is provided with a slide rail for the slider to slide up and down. Blocks are provided at the upper and lower ends of the slide rail to block the slider from sliding. The electromagnet is installed inside the slider. The slide rail, the block, the operating lever, and the washing tub are all insulators.

[0006] Preferably, the decalcification solution adding mechanism includes a U-shaped connector, a decalcification solution storage tank, and a replenishment pipe. The replenishment pipe has several outlets and is connected to the decalcification solution storage tank through the U-shaped connector. A decalcification solution drain outlet is provided on the lower part of the side wall of the decalcification chamber.

[0007] Preferably, the transverse partition is divided into eight blades by an inscribed square and two diagonals of the square. One end of the arc-shaped blade is fixed inside the washing tub, and the other end is connected to the triangular blade via a hinge. The triangular blade is movable upwards. A 0.5cm wide rising wedge-shaped silicone strip is provided on the bottom side of the joint between the triangular blade and the arc-shaped blade. A 0.5cm wide descending wedge-shaped silicone strip is provided on the right side of the triangular blade. When closed, the wedge-shaped silicone strip covers the adjacent triangular blade to completely cover the gap of the triangular blade. The triangular blade has a square hole with a side length of one-quarter at the connection of the adjacent bottom corners near the slide rail, allowing the operating rod to pass freely. The hole is covered with a cover, which consists of two small triangular blades that occupy one-quarter of the side length of the triangular blade. One side of the small triangular blade is connected to the triangular blade by a hinge, and the other two sides are provided with 0.3cm wide wedge-shaped silicone strips. The cover can be opened and closed upwards and completely covers the hole when closed. When the electromagnet attracts the sample chamber to move upwards, the sample chamber pushes open the four movable blades to form a channel for the sample chamber to move upwards. At the same time, the inner part of the operating rod barrel pushes open the cover to form a channel for the inner part of the operating rod barrel to move upwards.

[0008] Preferably, the blocking block is located at the top and bottom of the slide rail.

[0009] Preferably, the rocking mechanism includes a drive disc, a tray, and rocking rods. Four rocking rods are eccentrically connected to the drive disc on one side and connected to the four corners of the tray on the other side. A motor is installed inside the machine body. Two drive rods are symmetrically installed inside the machine body. The drive discs are fixedly installed at both ends of the drive rods. The motor is connected to a motor shaft gear through a motor shaft. The motor shaft gear meshes with a speed-changing gear. The speed-changing gear is installed on one drive rod. A first crown gear is fixedly installed on the drive rod with the speed-changing gear, and a second crown gear is fixedly installed on the other drive rod. A transmission shaft is rotatably installed inside the machine body. Two third crown gears are symmetrically installed at both ends of the transmission shaft. The two third crown gears are perpendicularly meshed with the first crown gear and the second crown gear, respectively.

[0010] Compared with the prior art, the present invention has the following advantages:

[0011] The present invention has a scientific and reasonable structural design, is simple to operate, and has low manufacturing cost. It can combine decalcification, rinsing, heating and shaking into one unit, which improves practicality, reduces experimental operation steps, facilitates experimental operation, and improves experimental efficiency.

[0012] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of the present invention.

[0014] Figure 2 This is a schematic diagram of the lifting device structure of the present invention.

[0015] Figure 3 This is a top view of the transverse partition structure in this invention.

[0016] Figure 4 This is a schematic diagram of the connection structure between the triangular blade and the arc-shaped blade in this invention.

[0017] Figure 5 This is a schematic diagram of the shaking table in this invention. Figure 6 This is a top view of the internal structure of the shaking machine body of the present invention.

[0018] Explanation of reference numerals in the attached figures:

[0019] 1—Sample chamber; 2—Washing tank; 3—Shaking table; 4—Rinsing chamber; 5—Decalcification chamber; 6—Divider; 7—Movable cover; 8—U-shaped connector; 9—Decalcification solution storage tank; 10—Replenishment pipe; 11—Heating ring; 12—Water inlet; 13—Drain outlet; 14—Tray; 15—Shaking table body; 16—Swing rod; 17—Operating lever; 18—Slider; 19—Electromagnet; 20—Slide rail; 21—Blocking block; 22— 23—Decalcification solution drain outlet; 24—Arc-shaped blade; 25—Triangular blade; 26—Hinge; 27—Rising wedge-shaped silicone strip; 28—Descending wedge-shaped silicone strip; 29—Hole; 30—Motor; 31—Motor shaft; 32—Motor shaft gear; 33—Speed-changing gear; 34—First crown gear; 35—Third crown gear; 36—Drive rod; 37—Drive disc; 38—Transmission shaft; 39—Second crown gear. Detailed Implementation

[0020] like Figures 1 to 6 As shown, the present invention includes a sample chamber 1, a washing tank 2, and a shaking table 3. A lifting device is slidably mounted on the washing tank 2 and is magnetically connected to the sample chamber 1. The upper part of the washing tank 2 is a rinsing chamber 4, and the lower part is a decalcification chamber 5. The rinsing chamber 4 and the decalcification chamber 5 are separated by a horizontal partition 6. A movable cover 7 is provided on the top of the sample chamber 1. The lower four-fifths of the side wall of the sample chamber 1 is a mesh plate. A decalcification liquid adding mechanism is connected to the decalcification chamber 5. A heating ring 11 is provided at the bottom of the decalcification chamber 5. A water inlet 12 and a water outlet 13 are provided on the rinsing chamber 4. The shaking table 3 is connected to the bottom of the decalcification chamber 5.

[0021] In this embodiment, the lifting device includes an operating lever 17, a slider 18, and an electromagnet 19. The washing tub 2 is provided with a slide rail 20 for the slider 18 to slide up and down. The upper and lower ends of the slide rail 20 are provided with blocking blocks 21 to block the slider 18 from sliding. The electromagnet 19 is disposed inside the slider 18.

[0022] The decalcification solution adding mechanism includes a U-shaped connector 8, a decalcification solution storage tank 9, and a replenishment pipe 10. One end of the U-shaped connector 8 is connected to the decalcification solution storage tank 9, and the other end is connected to the replenishment pipe 10 inside the decalcification chamber 5. The replenishment pipe 10 is provided with several water outlet holes, and the height of the replenishment pipe is flush with the top of the decalcification solution storage tank 9. A decalcification solution outlet 22 is opened at the lower part of the side wall of the decalcification chamber 5.

[0023] The transverse partition 6 is divided into eight blades by an inscribed square and two diagonals of the square. The eight blades include four arc-shaped blades 23 on the perimeter and four triangular blades 24 in the center. One end of each arc-shaped blade 23 is fixed to the side wall of the rinsing tub 2, and the other end is connected to the triangular blades 24 via a hinge 25. The triangular blades 24 can be flipped upwards. A 0.5cm wide rising wedge-shaped silicone strip 26 is provided on the bottom side of the joint between the triangular blades 24 and the arc-shaped blades 23. A 0.5cm wide descending wedge-shaped silicone strip 27 is provided on the right side of the triangular blades. A square hole 28 with a side length of one-quarter of the bottom corner of the triangular blades 24 is provided near the slide rail end, allowing the operating rod 17 to pass freely. A hole cover 29 is provided on the hole 28.

[0024] The hole cover 29 is composed of two small triangular blades. One side of the small triangular blade is connected to the triangular blade 24 by a hinge 25, and the other two sides are provided with wedge-shaped silicone strips 26. The hole cover can be opened and closed to form a channel for the inner part of the operating rod 17 to move upward, and when closed, it completely covers the hole 28. After the four triangular blades 24 are opened, they form a channel for the sample chamber 1 to move upward.

[0025] In this embodiment, the blocking block 21 is located at the top and bottom of the slide rail 20, and the blocking block is an insulator.

[0026] In this embodiment, the rocking mechanism includes a drive disc 37, a tray 14, and rocking rods 16. Four rocking rods 16 are eccentrically connected to the drive disc 37 on one side and connected to the four corners of the tray 14 via spherical heads on the other side. A motor 30 is installed inside the body 15. Two drive rods 36 are symmetrically installed inside the body 15. The drive disc 37 is fixedly installed at both ends of the drive rods 36. The motor 30 is connected to a motor shaft gear 32 via a motor shaft 31. The motor shaft gear 32 meshes with a speed-changing gear 33. The speed-changing gear 33 is installed on one drive rod 36. A first crown gear 34 is fixedly installed on the drive rod 36 on which the speed-changing gear 33 is installed, and a second crown gear 39 is fixedly installed on the other drive rod 36. A transmission shaft 38 is rotatably installed inside the body 15. Two third crown gears 35 are symmetrically installed at both ends of the transmission shaft 38. The two third crown gears 35 are perpendicularly meshed with the first crown gear 34 and the second crown gear 39, respectively. The motor shaft gear 32 and the speed-changing gear 33 are spur gears. When the motor 30 rotates, it drives the drive rod 36 to rotate through the speed-changing gear 33. The first crown gear 34 on the drive rod 36 rotates, and the vertical transmission of the crown gear 34 changes the transmission direction to drive the second crown gear 39 to rotate, ultimately driving both drive rods 36 to rotate.

[0027] In use, open the movable chamber cover 7, place the tissue specimen into the sample chamber 1, close the sample chamber cover 7, place the sample chamber 1 into the decalcification chamber 5, fill the decalcification solution storage tank 9 with decalcification solution, and let the decalcification solution flow through the U-shaped connector 8 to the appropriate position in the decalcification chamber 5. Start the motor 30, which drives the motor shaft gear 32 to rotate through the motor shaft 31, which in turn drives a drive rod 36 to rotate. The power is then transmitted to another drive rod 36 through two third crown gears 35. The drive rod 36 drives the drive discs 37 at both ends to rotate. The four drive discs 37 rotate simultaneously, causing the four rocking rods 16 to move and achieve the purpose of shaking the tray 14. The shaker then begins to work. The energizing time of electromagnet 19 is set according to the decalcification time used for the sample type. During the decalcification process, heating ring 11 heats the decalcification liquid at a constant temperature of 37°C. During the decalcification process, the decalcification liquid will evaporate and decrease to a certain extent. U-shaped communicating vessel 8 continuously replenishes the decalcification liquid using pressure difference. After decalcification is completed, electromagnet 19 is energized, slider 18 adsorbs sample chamber 1, and water outlet 22 is activated to discharge the decalcification liquid. The sample chamber moves upward through the operating rod 17, passes through hole 28 and breaks through hole cover 29. At the same time, it drives the sample chamber to break through the transverse partition 6 and the blades open upward. After the sample chamber passes through the transverse partition 6 smoothly, it is transported to the rinsing chamber 4. The blades fall freely due to gravity, and partition 6 and hole cover 29 close again. The silicone on the blades completely covers the gaps between the blades, so that the rinsing layer forms a near-sealed space. Then the power is cut off, sample chamber 1 falls into rinsing chamber 4, water inlet 12 opens, tap water rushes into the rinsing layer, and water outlet 13 drains water to achieve rinsing. After 24 hours, the inlet 12 is closed, and excess liquid is discharged from the outlet 13. The operator then removes the sample chamber for subsequent experiments. The above description is merely a preferred embodiment of the present invention and does not constitute any limitation on the invention. Any simple modifications, alterations, or equivalent changes made to the above embodiments based on the inventive technical essence shall still fall within the protection scope of the present invention.

Claims

1. An automatic decalcification and flushing integrated machine, characterized in that, The sample chamber includes a sample chamber (1), a washing tank (2), and a shaking table (3). A lifting device is slidably mounted on the washing tank (2), and the lifting device is magnetically connected to the sample chamber (1). The upper part of the washing tank (2) is a rinsing chamber (4), and the lower part is a decalcification chamber (5). The rinsing chamber (4) and the decalcification chamber (5) are separated by a partition (6). The sample chamber (1) is a cubic container. A movable cover (7) is provided on the top of the sample chamber (1). The lower four-fifths of the side wall of the sample chamber (1) is a mesh plate. The bottom of the decalcification chamber (5) is connected to... The decalcification chamber (5) is connected to a decalcification liquid adding mechanism. The decalcification liquid adding mechanism is connected to the decalcification liquid storage tank (9) and the replenishment pipe (10) by a U-shaped connector (8). A heating ring (11) is set at the bottom of the decalcification chamber (5). An inlet (12) and a drain (13) are set on the rinsing chamber (4). The shaker (3) is composed of a tray (14) and a machine body (15). The upper surface of the tray (14) is connected to the bottom of the washing tank (2). The tray (14) and the machine body (15) are connected by a swing mechanism. The diaphragm (6) is divided into eight blades by its inscribed square and two diagonals. The eight blades specifically include four arc-shaped blades (23) around the perimeter and four triangular blades (24) in the middle. One end of the arc-shaped blade (23) is fixed to the side wall of the decalcification chamber (5), and the other end is connected to the triangular blade (24) through a hinge (25). The triangular blade (24) can be flipped upward. A 0.5cm wide rising wedge-shaped silicone strip (26) is provided on the bottom side of the joint between the triangular blade (24) and the arc-shaped blade (23), and a 0.5cm wide descending wedge-shaped silicone strip (27) is provided on the right side of the triangular blade (24). Near the end of the slide rail, a square hole (28) with a side length of one-quarter of the length of the triangular blade (24) is left at the connection of a pair of adjacent bottom corners, allowing the operating rod (17) to pass freely. The hole (28) is provided with a hole cover (29), which is composed of two small triangular blades. One side of the small triangular blade is connected to the triangular blade (24) by a hinge (25), and the other two sides are provided with wedge-shaped silicone strips (27). The hole cover can be opened and closed to form a channel for the operating rod (17) to move upward inside the barrel, and when closed, it completely covers the hole (28). When the four triangular blades (24) are opened, they form a channel for the sample chamber (1) to move upward. The rocking mechanism includes a drive disc (37), a tray (14), and rocking rods (16). Four rocking rods (16) are eccentrically connected to the drive disc (37) on one side and connected to the four corners of the tray (14) on the other side. A motor (30) is installed inside the body (15). Two drive rods (36) are symmetrically installed inside the body (15). The drive discs (37) are fixedly installed at both ends of the drive rods (36). The motor (30) is connected to a motor shaft gear (32) via a motor shaft (31). The motor shaft gear (32) meshes with a variable... The gear (33) is mounted on a drive rod (36). A first crown gear (34) is fixedly mounted on the drive rod (36) on which the gear (33) is mounted, and a second crown gear (39) is fixedly mounted on another drive rod (36). A transmission shaft (38) is rotatably mounted inside the body (15). Two third crown gears (35) are symmetrically mounted at both ends of the transmission shaft (38). The two third crown gears (35) are perpendicularly meshed with the first crown gear (34) and the second crown gear (39).

2. The automatic decalcification and flushing integrated machine according to claim 1, characterized in that, The lifting device includes an operating lever (17), a slider (18), and an electromagnet (19). The washing tank (2) is provided with a slide rail (20) for the slider (18) to slide up and down. The upper and lower ends of the slide rail (20) are provided with blocking blocks (21) to block the slider (18) from sliding. The electromagnet (19) is located inside the slider (18). The outer wall of the sample chamber (1) is provided with a magnetic metal block that is attracted to the electromagnet (19).

3. The automatic decalcification and flushing integrated machine according to claim 2, characterized in that, The blocking block (21) is located at the top and bottom of the slide rail (20).

4. The automatic decalcification and flushing integrated machine according to claim 1, characterized in that, One end of the U-shaped connector (8) is connected to the decalcification liquid storage tank (9), and the other end is set in the decalcification chamber (5) and connected to the replenishment pipe (10). The replenishment pipe (10) is provided with several water outlet holes. The height of the replenishment pipe (10) is flush with the top of the decalcification liquid storage tank (9). The lower part of the side wall of the decalcification chamber (5) is provided with a decalcification liquid outlet (22).

Citation Information

Patent Citations

  • Flushing device of decalcified orthopedics case sample

    CN107855306A

  • Rapid decalcification kit for bone and cartilage tissues, and preparation method of kit

    CN109323907A