Biological control use of schisandra chinensis and its biological control agent, preparation method and biological control method

Abstract

The present invention "biological control use of tricholoma spatholobi and its biological control preparation, preparation method and biological control method" belongs to the field of biological control technology. The present invention provides the biological control use of tricholoma spatholobi and the use of preparing biological control preparations, and based on these uses, the present invention further provides a biological control preparation with tricholoma spatholobi essential oil as an active ingredient and its preparation method and biological control method. The tricholoma spatholobi essential oil provided by the present invention can effectively kill western flower thrips, entomophilous book lice, and two-spotted spider mites, and is non-toxic and harmless, does not produce pollution, is safe and reliable, low-cost, environmentally friendly and efficient.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and specifically relates to the biological control use of sharp sticky hairy fragrant green and its biological control preparation, preparation method and biological control method. Background Art

[0002] From ancient times to the present, pests and diseases have always been an important limiting factor in agricultural development, and people have been looking for ideal prevention and control methods. Food production includes pre-production (seeds), production (planting) and post-production (storage) processes. According to the World Food and Agriculture Organization (FAO), the losses caused by pests and diseases during the planting process account for about 1 / 3 of the world's agricultural output (14% for pests; 10% for diseases; 11% for weeds). The average annual grain storage loss in the world accounts for 10% of grain production, and some underdeveloped countries even reach 30%. A survey conducted by the State Administration of Grain in my country shows that the current grain storage of farmers in my country is about 500 billion catties. Due to improper storage, the annual loss is about 8% on average, up to 40 billion catties, which is equivalent to the annual output of a medium-sized grain-producing province. Therefore, depriving grain of insects has become an important research topic. At present, the control of pests and diseases in agricultural production still mainly relies on chemical pesticides, and fumigants such as phosphine are mainly used for grain storage.

[0003] Due to the long-term irrational abuse of chemical pesticides, the environment has been polluted, the ecological balance of nature has been destroyed, and human health has been endangered, which is the so-called "3R" hazard. That is, pesticide residues (Residue); pests have resistance to chemical pesticides (Resistance); and pests have become rampant (Resurgence). At the same time, many highly toxic pesticides also cause serious harm to producers and users. Therefore, it is urgent to research and develop green pesticides that are efficient, low-toxic, and environmentally friendly. Screening and discovering active botanical pesticides from plants has become a hot topic in the world's scientific community. Botanical pesticides are substances with pesticide activity extracted from plants. Active substances can become a development template for innovative pesticides and further developed into new pesticides.

[0004] Western flower thrips, also known as alfalfa thrips (Frankliniella occidentalis), belongs to the family Thripidae of the order Thysanoptera. It is a dangerous pest worldwide and an important quarantine pest internationally. It is native to North America and has a wide range of hosts and a diverse diet. Its host plants include more than 500 important economic crops and food crops such as potatoes, eggplants, tomatoes, peppers, cucumbers, beans, bananas, mangoes, plums, peaches, apples, grapes, strawberries, and flowers (orchids and chrysanthemums). With the continuous spread of western flower thrips, its host species are still increasing. Western flower thrips feed on the stems, leaves, flowers, and fruits of plants with rasping and sucking mouthparts, causing petals to fade, leaves to shrink, and stems and fruits to form scars, which may eventually cause the plants to wither. At the same time, it also spreads a variety of plant viruses including tomato spotted wilt virus, posing a serious threat to agricultural production. Because western flower thrips are small in size, their harmful activities are hidden, they can reproduce parthenogenetically, have strong reproductive capacity, and their population grows rapidly. Since western flower thrips have developed different degrees of resistance to insecticides such as organophosphates, carbamates, pyrethroids and spinosad, it is urgent to find green and pollution-free insecticides that are effective against western flower thrips in production.

[0005] The two-spotted spider mite (Tetranychus urticae) is a worldwide agricultural pest mite belonging to the genus Acarina of the order Acarina of the class Arachnida. It is also known as the cotton red spider and the white spider. It mainly lives on green plants in early spring on the ground. It is carried and spread by wind, flowing water, insects, birds, beasts, humans and livestock, various agricultural tools and flower seedlings. The host plants of the two-spotted spider mite are very wide, including various vegetables, fruit trees, corn, beans and more than 1,000 plants from more than 50 families. Both adults and nymphs of the two-spotted spider mite can cause damage. In the early stage of the occurrence, the pest mites mostly gather on both sides of the main veins on the back of the leaves, inhibiting the normal photosynthesis. The two sides of the veins on the front of the affected leaves show chlorosis. When the insect population density reaches a certain number, it will spin silk webs at the top of the plant and spread outward through fine filaments, damaging the leaf surface and the green parts of the host plant such as the tender stems, stamens, and fruit stalks, causing the entire plant to turn yellow and die. In addition, the mite also releases toxins or growth regulators, causing imbalance in plant growth, so that some young leaves appear uneven and damaged. When the disease breaks out, leaves, weeds, and crop leaves are all scorched. The prevention and control of two-spotted spider mites mainly relies on the application of chemically synthesized miticides. Due to long-term use, two-spotted spider mites have developed high resistance to commonly used miticides, such as avermectin, pyridabenzyl, cypermethrin, and thiamethoxam, and the recommended doses are basically ineffective. At the same time, the use of chemical agents also has a killing effect on non-target organisms, destroying the ecological balance and causing the mites to become rampant again. There is an urgent need to discover efficient and environmentally friendly miticides.

[0006] Liposcelis entomophila is an important stored grain pest belonging to the order Gnathophoridae of the class Insecta. Liposcelis entomophila can live among grains, plants, under bark, on lichens and old wood. It is tiny (1-10 mm), has a short development period, a long life span of adults, has a miscellaneous diet, is highly resistant to starvation, and reproduces very quickly. When it occurs in large numbers, it can cause serious economic losses and is extremely harmful to stored goods such as grain, food, feed and medicinal materials. It can even cause local grain moisture to increase, leading to fever and mildew. Because the excrement attached to its body can carry and spread pathogens and cause allergies, and because it is highly active, it can cause dermatitis and asthma in humans, posing a threat to human health. The use of traditional aluminum phosphide fumigation not only fails to completely eliminate book lice, but often also increases their resistance, causing the pests to become rampant again. Practice has shown that the prevention and control of book lice is very difficult. Whether it is a granary treated with controlled atmosphere or fumigated with chemicals, the last remaining and first discovered pests are mainly book lice. Therefore, finding new safe and environmentally friendly pesticide substitutes is an urgent problem to be solved.

[0007] Anaphalis bulleyana, also known as five-spice grass, is an annual or biennial herb of the Asteraceae family, with thick taproots, rosette leaves and solitary or a few tufted flower stems, all with long, cottony hairs in a spider web shape, with rusty brown, adherent glandular hairs. The stem is erect, grooved, usually branched, usually glabrous at the bottom and densely hairy at the top. The rosette leaves are obovate, up to 9 cm long, and gradually narrow to a wing-like short petiole at the bottom; the leaves at the bottom of the stem wither during flowering, the middle and upper leaves are oblanceolate or obovate-spatulate, 3.5-10 cm long, extending along the stem to form a wedge-shaped wide wing: the upper leaves are linear-lanceolate; there are many capitulums, which are densely packed into compound corymbs at the ends of the stems and branches; the involucre is obovate, 5-6 mm long, with 4-5 layers of involucral bracts, erect, membranous, transparent, light yellow at the bottom, not spreading; the outer layer is ovate-oblong, the inner layer is long-spatulate, 5-6 mm long, and the innermost layer is broadly linear with long claws; the achenes are oblong with microgland. The flowering period is from August to September, and the fruiting period is from September to October. It is produced in subalpine humid slopes and low mountain grasslands (1180-3300m) from western Sichuan, northwestern and northern Yunnan (Lijiang, Zhongdian, Kunming, Dongchuan, etc.) to central Guizhou.

[0008] The whole plant is also a Chinese medicinal material, and the Yunnan Chinese Herbal Medicine states that it is pungent and warm in nature. Functions and indications: anti-inflammatory and analgesic, stomach-strengthening and qi-promoting. It can treat tonsillitis, acute gastroenteritis, cystitis, urethritis, and infantile malnutrition.

[0009] At present, there is no research report on the chemical composition of the essential oil of Rhizoma Cibotii in this field, and there is no report that Rhizoma Cibotii can be used in the field of biological control to kill pests and mites. Summary of the invention

[0010] In order to solve the problems of hazards and defects of the above-mentioned chemical pesticides in the prior art in the field, provide a new type of biological pesticide for controlling pests / mites, and fill the application gap of the above-mentioned sticky fragrant green in the field, the present invention provides the biological control use of sticky fragrant green and its biological control preparation, preparation method and biological control method.

[0011] The technical contents of the present invention are as follows:

[0012] The biological control uses of sticky-hair fragrant green.

[0013] The biological control refers to the use of linden essential oil to control pests or mites;

[0014] Preferably, the pest is selected from: western flower thrips, entomophilous book lice;

[0015] Preferably, the harmful mite is Tetranychus urticae.

[0016] Use of the sticky-hair fragrant green to prepare biological control preparations.

[0017] Using the essential oil of Psoralea corylifolia as the active ingredient of the biocontrol agent;

[0018] Preferably, the biological control refers to the use of the essential oil of Cistanche deserticola to control pests or mites;

[0019] Preferably, the pest is selected from: western flower thrips, entomophilous book lice;

[0020] Preferably, the harmful mite is Tetranychus urticae.

[0021] A biocontrol agent comprises an active ingredient, wherein the active ingredient comprises scutellaria baicalensis essential oil.

[0022] The biological control agent also includes auxiliary materials.

[0023] A method for preparing a biocontrol agent comprises: using scutellaria baicalensis essential oil as an active ingredient of the biocontrol agent.

[0024] The linalool essential oil is an essential oil obtained by extracting an oil-water mixture obtained by boiling the aerial part of the linalool plant in water;

[0025] Preferably, the boiling distillation time is 6 hours;

[0026] Preferably, before extraction, the oil-water mixture is condensed, recovered and then extracted;

[0027] Preferably, the extraction temperature is 25-30°C, preferably 25°C;

[0028] Preferably, drying is performed after the extraction is completed;

[0029] Preferably, the drying refers to dehydration by adding anhydrous sodium sulfate;

[0030] Preferably, after drying, the extract is evaporated and concentrated using a rotary evaporator at 40° C. to remove the extractant;

[0031] Preferably, the extraction agent of the extraction is petroleum ether;

[0032] Preferably, the method for preparing the biocontrol agent further comprises: adding auxiliary materials.

[0033] A biological control method adopts the essential oil of scutellaria baicalensis to carry out biological control.

[0034] The biological control refers to the use of linden essential oil to control pests or mites;

[0035] Preferably, the control refers to killing pests or mites by fumigation or contact killing with the essential oil of tricholoma glutinosus, or repelling pests with the essential oil of tricholoma glutinosus;

[0036] Preferably, the pest is selected from: western flower thrips, entomophilous book lice;

[0037] Preferably, the harmful mite is Tetranychus urticae;

[0038] Preferably, the median lethal concentration of the essential oil of C. glutinosus to Tetranychus urticae is 8.23 ​​mg / mL;

[0039] Preferably, the median lethal concentration of the essential oil of C. glutinosae to western flower thrips is 5.01 mg / mL;

[0040] Preferably, the median lethal concentration of the essential oil of the sticky hair fragrant green for fumigation of insectivorous book lice is 0.27 mg / L, and the median lethal dose for contact killing of insectivorous book lice is 157.41 μg / cm 2 ;

[0041] Preferably, the repellent concentration of the essential oil of Acanthus viscidus to the entomophilous book lice is 1.0-8.0 μg / cm 2 .

[0042] The beneficial effects of the present invention are as follows:

[0043] The present invention unexpectedly discovered that the plant tricholoma ciliata has a lethal effect on western flower thrips, insectivorous book lice, and two-spotted spider mites, and verified its outstanding effect in the field of biological control. Therefore, the present invention requests protection for the biological control use of tricholoma ciliata, the use of tricholoma ciliata to prepare biological control preparations, biological control preparations with tricholoma ciliata essential oil as an active ingredient and its preparation method, as well as a biological control method. The preparation of tricholoma ciliata essential oil uses the above-ground part of the tricholoma ciliata plant as a raw material, and the tricholoma ciliata essential oil is prepared by boiling, extracting, distilling and other steps. At the same time, the present invention also provides a biological control preparation based on tricholoma ciliata essential oil, which can effectively kill western flower thrips, insectivorous book lice, and two-spotted spider mites, and is non-toxic and harmless, and does not produce pollution. It is a safe, reliable, low-cost, environmentally friendly and efficient new biological control preparation. The tricholoma is widely distributed in western Sichuan, northwestern and northern Yunnan (Lijiang, Zhongdian, Kunming, Dongchuan, etc.), and central Guizhou. It can be easily obtained in large quantities. Using the essential oil of the tricholoma to control pests and mites is in line with the concept of green and environmentally friendly biological control. DETAILED DESCRIPTION

[0044] The details of the present invention are further described below in conjunction with specific embodiments and experimental examples, but the protection scope of the present invention is not limited thereto.

[0045] Sources of biological materials

[0046] 1. The lindera used in Experimental Example 1 of the present invention was collected from Longma Mountain, Hongta District, Yuxi City, Yunnan Province, and was identified by Professor Liu Quanru of the School of Life Sciences of Beijing Normal University. The public can obtain wild lindera plants in western Sichuan, central and northwestern Yunnan, and southeastern Tibet, and use them to reproduce the technical solution of the present invention and verify the technical effect of the present invention.

[0047] 2. The two-spotted spider mites used in Experimental Example 2 of the present invention were artificially bred in the applicant's laboratory. The public can collect and obtain the two-spotted spider mites from any area where two-spotted spider mite diseases occur and reproduce the technical solution of the present invention to verify the technical effect of the present invention.

[0048] 3. The western flower thrips used in Experimental Example 3 of the present invention were artificially bred in the applicant's laboratory. The public can collect western flower thrips from any area where western flower thrips pests occur and reproduce the technical solution of the present invention to verify the technical effect of the present invention.

[0049] 4. The entomophilous book lice used in Experimental Examples 4, 5 and 6 of the present invention were artificially bred in the applicant's laboratory. The public can collect entomophilous book lice from any place where entomophilous book lice infestation occurs and reproduce the technical solution of the present invention to verify the technical effect of the present invention.

[0050] The first group of embodiments, new uses of the present invention's sticky hair fragrant green

[0051] This group of embodiments provides biological control uses of the sticky-hairy fragrant green.

[0052] In some embodiments, the biological control refers to controlling insect pests or mites with the essential oil of Folium glutinosae;

[0053] Preferably, the pest is selected from: western flower thrips, entomophilous book lice;

[0054] Preferably, the harmful mite is Tetranychus urticae.

[0055] Based on the teachings of the present invention, those skilled in the art can select any tissue or part of the plant of the tricholoma, including but not limited to roots, stems, leaves, flowers, fruits, and seeds for control of pests / mites, or extract any tissue or part of the plant of the tricholoma, including but not limited to roots, stems, leaves, flowers, fruits, and seeds or use the extracted substances to control pests / mites.

[0056] Any act of selecting any tissue or part of the plant of C. glutinosa, including but not limited to roots, stems, leaves, flowers, fruits, and seeds for pest / mite control, or any act of extracting any tissue or part of the plant of C. glutinosa, including but not limited to roots, stems, leaves, flowers, fruits, and seeds or using the extracted substances to control pests / mites, or any act of preparing pest / mite control products from any tissue or part of the plant of C. glutinosa, including but not limited to roots, stems, leaves, flowers, fruits, and seeds, or using, selling, promising to sell, or importing the pest / mite control products, or any act of extracting any tissue or part of the plant of C. glutinosa, including but not limited to roots, stems, leaves, flowers, fruits, and seeds or using, selling, promising to sell, or importing the pest / mite control products, all fall within the scope of protection of the present invention.

[0057] The controlled pests / mites are not limited to western flower thrips, entomophilous book lice, and two-spotted spider mites, but may also be other pests / mites other than western flower thrips, entomophilous book lice, and two-spotted spider mites.

[0058] The second group of embodiments, use of the present invention's sticky hairy fragrant green to prepare the biological control preparation

[0059] This group of embodiments provides the use of Rhizoma Cibotii to prepare biocontrol agents.

[0060] In a specific embodiment, the essential oil of Psoralea corylifolia is used as the active ingredient of the biocontrol agent;

[0061] Preferably, the biological control refers to the use of the essential oil of Cistanche deserticola to control pests or mites;

[0062] Preferably, the pest is selected from: western flower thrips, entomophilous book lice;

[0063] Preferably, the harmful mite is Tetranychus urticae.

[0064] Based on the teachings of the present invention, those skilled in the art can select any tissue or part of the plant of C. glutinosa, including but not limited to roots, stems, leaves, flowers, fruits, and seeds to control pests / mites, control western flower thrips, insectivorous book lice, two-spotted spider mites, or pests / mites other than western flower thrips, insectivorous book lice, and two-spotted spider mites, or extract any tissue or part of the plant of C. glutinosa, including but not limited to roots, stems, leaves, flowers, fruits, and seeds to control pests / mites, control pests / mites other than western flower thrips, insectivorous book lice, and two-spotted spider mites.

[0065] Any act of selecting any tissue or part of the plant of Glechoma longituba, including but not limited to roots, stems, leaves, flowers, fruits, and seeds to control pests / mites, or any act of extracting any tissue or part of the plant of Glechoma longituba, including but not limited to roots, stems, leaves, flowers, fruits, and seeds, or the extracted substances to control pests / mites, or any act of preparing biological control products from any tissue or part of the plant of Glechoma longituba, including but not limited to roots, stems, leaves, flowers, fruits, and seeds, or using, selling, promising to sell, or importing the biological control products, or any act of extracting any tissue or part of the plant of Glechoma longituba, including but not limited to roots, stems, leaves, flowers, fruits, and seeds, or preparing biological control products from substances obtained, or using, selling, promising to sell, or importing the biological control products, all fall within the scope of protection of the present invention.

[0066] The third group of examples, the biocontrol agent of the present invention

[0067] This group of embodiments provides a biocontrol agent. All embodiments of this group have the following common features: the biocontrol agent comprises: an active ingredient, and the active ingredient comprises the essential oil of Scutellaria baicalensis.

[0068] In a further embodiment, the biocontrol agent further comprises: auxiliary materials.

[0069] In a specific embodiment, the excipients are pharmaceutically acceptable excipients, including but not limited to: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, adhesives, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, integrities, penetration enhancers, pH regulators, buffers, plasticizers, surfactants, foaming agents, defoamers, thickeners, inclusion agents, humectants, absorbents, diluents, flocculants, deflocculating agents, filter aids, release retardants, etc.

[0070] The fourth group of embodiments, the preparation method of the biocontrol agent of the present invention

[0071] This group of embodiments provides a method for preparing a biocontrol agent. All embodiments in this group have the following common features: the method for preparing a biocontrol agent comprises: using the essential oil of Psoralea corylifolia as an active ingredient of the biocontrol agent.

[0072] In a specific embodiment, the essential oil of the Herba Lysimachiae is an essential oil obtained by extracting an oil-water mixture obtained by boiling the aerial part of the Herba Lysimachiae plant;

[0073] Preferably, the boiling distillation time is 6 hours;

[0074] Preferably, before extraction, the oil-water mixture is condensed, recovered and then extracted;

[0075] Preferably, the extraction temperature is 25-30°C, preferably 25°C;

[0076] Preferably, drying is performed after the extraction is completed;

[0077] Preferably, the drying refers to dehydration by adding anhydrous sodium sulfate;

[0078] Preferably, after drying, the extract is evaporated and concentrated using a rotary evaporator at 40° C. to remove the extractant;

[0079] Preferably, the extraction agent of the extraction is petroleum ether;

[0080] Preferably, the method for preparing the biocontrol agent further comprises: adding auxiliary materials.

[0081] In a specific embodiment, the excipients are pharmaceutically acceptable excipients, including but not limited to: solvents, propellants, solubilizers, cosolvents, emulsifiers, colorants, adhesives, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, integrities, penetration enhancers, pH regulators, buffers, plasticizers, surfactants, foaming agents, defoamers, thickeners, inclusion agents, humectants, absorbents, diluents, flocculants, deflocculating agents, filter aids, release retardants, etc.

[0082] The fifth group of embodiments, the biological control method of the present invention

[0083] This group of embodiments provides a biological control method. All embodiments in this group have the following common features: using the essential oil of scutellaria baicalensis for biological control.

[0084] In a specific embodiment, the biological control refers to the use of linalool essential oil to control pests or mites;

[0085] Preferably, the control refers to killing pests or mites by fumigation or contact killing with the essential oil of Cistanche deserticola;

[0086] Preferably, the pest is selected from: western flower thrips, entomophilous book lice;

[0087] Preferably, the harmful mite is Tetranychus urticae;

[0088] Preferably, the median lethal concentration of the essential oil of C. glutinosus to Tetranychus urticae is 8.23 ​​mg / mL;

[0089] Preferably, the median lethal concentration of the essential oil of C. glutinosae to western flower thrips is 5.01 mg / mL;

[0090] Preferably, the median lethal concentration of the essential oil of the sticky hair fragrant green for fumigation of insectivorous book lice is 0.27 mg / L, and the median lethal dose for contact killing of insectivorous book lice is 157.41 μg / cm 2 ;

[0091] Preferably, the repellent concentration of the essential oil of Acanthus viscidus to the entomophilous book lice is 1.0-8.0 μg / cm 2 .

[0092] Experimental Example 1, Preparation of the essential oil of the present invention

[0093] 1. Source of test insects: Western flower thrips, two-spotted spider mites and entomophilous book lice were artificially bred.

[0094] 2. Plant source: Anaphalis bullleyana is a wild plant. Its above-ground part (3 kg) was collected in Yuxi City, Yunnan Province in August 2022, and was identified as Anaphalis bullleyana by Professor Liu Quanru of the School of Life Sciences of Beijing Normal University.

[0095] 3. Preparation of the essential oil of Rhizoma Cibotii

[0096] The fresh aerial parts of the scutellaria baicalensis (in the flowering period) were collected, cut into small pieces of 3-5 cm, and treated with water boiling method for 6 hours. The obtained oil-water mixture was condensed and recovered, extracted with n-hexane, dried with anhydrous sodium sulfate, and the n-hexane was removed by rotary evaporator to obtain the essential oil of the scutellaria baicalensis. The water bath temperature was 40°C.

[0097] The yield of fresh scutellaria glutinosa essential oil is 0.25% (w / w), that is, 0.25g of scutellaria glutinosa essential oil can be extracted from 100g of fresh scutellaria glutinosa plants. The specific gravity of the essential oil is 0.92g / mL.

[0098] Experimental Example 2: Determination of the contact activity of the essential oil of Rhizoma Cibotii against Tetranychus urticae

[0099] The leaf disc method was used to determine the toxicity of the essential oil of tricholoma glutinosus to spider mites. The specific operation was as follows: 12-well cell culture plates were coated with polytetrafluoroethylene on the inner wall of the culture plates and dried with a hair dryer; water and agar were heated and mixed in a microwave oven at a ratio of 100:1.5, poured into the 12-well cell culture plates (about 2-3 mL per well) to a height of about 0.5 mm, and cooled until solidified; fresh kidney bean leaves that had not been treated with pesticides were taken, and holes were punched in the leaves with a 19 mm diameter puncher to make circular leaf discs; a certain amount of tricholoma glutinosus essential oil was dissolved in anhydrous ethanol (the amount did not exceed 1% of the liquid system), and dimethyl sulfoxide (DMSO) was added to dissolve the oil. no more than 1% of the liquid medicine system) to dissolve, prepare a mother solution, and then dilute it with distilled water to the required concentration to obtain the liquid medicine system; immerse the leaf disc in the agent for 10 seconds, take it out, dry it, put the kidney bean leaf with the liquid medicine on the culture plate with the back facing up, and inoculate 10 adult spider mites in each well; stick the hole side of the culture plate firmly with rice paper and put it in an incubator, the temperature is 26-28℃, the relative humidity is 65%-75%, and the photoperiod is L:D=16:8; observe and record the number of deaths under a stereoscope after 24 hours, touch the moving ones with a brush as alive, and the immobile ones as dead, and calculate the LD 50 .

[0100] Mortality rate (%) = number of deaths / number of test insects * 100

[0101] Corrected mortality rate (%) = (treatment group mortality rate - control group mortality rate) / (1 - control group mortality rate) * 100

[0102] The median lethal concentration (LC 50 ): refers to the concentration required to cause death of 50% of the test subjects. Calculated using Probit software.

[0103] The results are shown in Table 1 below:

[0104] Table 1. Toxicity of the essential oil of Atractylodes lanceolata to Tetranychus urticae determined by leaf disc method

[0105]

[0106] The LC50 of the essential oil of Psoralea corylifolia against Tetranychus urticae is 50 =8.23mg / mL, 95% confidence interval is (7.18-9.455) regression equation y = 2.90x-2.66. The half lethal concentration means that each milliliter of the liquid system (including distilled water, solvent-alcohol and co-solvent-dimethyl sulfoxide) contains 8.23mg of Vitex lindracena essential oil. At this concentration, half of the test individuals died.

[0107] Experimental Example 3: Determination of the activity of the essential oil of Atractylodes lanceolata against agricultural pests, Western Flower Thrips

[0108] The leaf disc method was used to determine the toxicity of hydrangea sticky hair fragrant green to western flower thrips. The specific operation was as follows: a certain amount of sticky hair fragrant green essential oil was dissolved in anhydrous ethanol (the amount did not exceed 1% of the liquid medicine system), Tween 80 (not more than 0.5% of the liquid medicine system) was added to assist dissolution, and a mother solution was prepared, which was then diluted with distilled water to the required concentration and the liquid medicine system was obtained for use; 1mL of the liquid medicine was placed in a 22mL glass scintillation bottle, rolled evenly for several circles to form a drug film, the liquid medicine was poured out, and the scintillation bottle was dried for use. Fresh lentils (pods) that had not been applied with pesticides were taken, and the lentils were made into 1cm square sections, immersed in the agent for 10s, taken out, dried, and placed in a scintillation bottle; 5 replicates were set for each treatment, and 10 second-instar nymphs of western flower thrips were inoculated in each replicate, and the bottle mouth was sealed with a 150-mesh insect-proof net; placed at room temperature, the results were observed after 24 hours, and the insect body was moved with a brush, and the insect body was recorded as dead if it did not move.

[0109] Mortality rate (%) = number of deaths / number of test insects * 100

[0110] Corrected mortality rate (%) = (treatment group mortality rate - control group mortality rate) / (1 - control group mortality rate) * 100

[0111] The median lethal concentration (LC 50 ): refers to the concentration required to cause death of 50% of the test subjects. Calculated using Probit software.

[0112] The results are shown in Table 2:

[0113] Table 2. Cytotoxic activity of linden essential oil against western flower thrips

[0114]

[0115]

[0116] The median lethal concentration of the essential oil of C. glutinosae to the second-instar nymphs of western flower thrips is LC 50 =5.01 mg / mL, 95% confidence interval is (4.37-5.78) regression equation y=2.82x-1.97. The median lethal concentration LC in Table 2 above 50 The meaning is: within 24 hours, the number of milligrams of linalool essential oil contained in each milliliter of the drug solution system (with alcohol, Tween 80 and distilled water as solvents) can reach 50% of the mortality rate of the test individuals treated with such concentration.

[0117] Experimental Example 4: Determination of the fumigation activity of the essential oil of Rhizoma Cibotii against stored-warehouse pests

[0118] Determination of fumigation activity of sticky hair fragrant green essential oil on insectivorous book lice: pick 10 test insects with a brush and put them into a small glass bottle with a diameter of 0.5cm and a height of 1.6cm (easy to observe the death of the test insects), the inner wall of the bottle mouth is coated with polytetrafluoroethylene to prevent the test insects from escaping, the small glass bottle is placed in a large ground glass bottle (290mL) with a height of 12cm and a diameter of 4cm, acetone is used as a solvent to dilute the agent into an equal ratio series, and 10 microliters of the agent dilution (6 concentrations) are drawn with a pipette and dripped on a filter paper strip (7.5cm long and 1.5cm wide), and the filter paper is glued to the ground bottle cap with solid glue, and the ground bottle cap is quickly tightened and wrapped with a sealing film for a week to seal. After 24 hours, observe and record the death of the test insects. Each concentration is repeated 5 times, and each repeat has 10 test insects. Acetone without adding agents is used as a blank control. Observe after 24 hours. Pour the test insects in the small glass bottle onto a piece of white paper and use a brush to move the insect body. If the insect does not move, it is dead.

[0119] Mortality rate (%) = 100 × number of dead insects / number of test insects.

[0120] Corrected mortality rate (%) = 100 × (mortality rate of treatment group - mortality rate of control group) / (1 - mortality rate of control group).

[0121] The median lethal dose (LC 50 ) is the dose that causes 50% mortality of entomophilous book lice, and the median lethal dose was calculated using the Probit software package.

[0122] The results are shown in Table 3:

[0123] Table 3. Fumigation activity of the essential oil of tricholoma scabra against entomophilous book lice determined by closed space method

[0124]

[0125]

[0126] The fumigation activity of the essential oil of Psoralea glutinosae against adults of the book lice was LC 50 = 0.27 mg / L air, 95% confidence interval is (0.23-0.32), regression equation y = 1.44 + 2.52x. The half lethal dose means: 0.27 mg of essential oil is contained in each liter of enclosed space. At this concentration, half of the test subjects die.

[0127] Experimental Example 5: Determination of contact activity of the essential oil of scutellaria baicalensis against storage pests

[0128] The contact killing activity of the essential oil of the sticky hair fragrant green was determined by the drug film method. The specific operation is as follows: use acetone as a solvent to prepare 5 concentrations of essential oil solutions obtained through preliminary tests; cut filter paper pieces with a diameter of 3.5 cm; evenly apply 150 μL of the test solution on the circular filter paper pieces with a diameter of 3.5 cm, wait for the acetone to evaporate, and use a solid glue stick to stick the filter paper pieces to a plastic six-well plate coated with a polytetrafluoroethylene aqueous solution, with 5 replicates; each well is a replicate, and 10 insectivorous book lice are placed in each well, and acetone is used as a blank control. Then place it in a dark incubator (28-30℃, 70-75% RH). After 24 hours of observation, pour the test insects in the small glass bottle onto a piece of white paper, and use a brush to poke the insect body. If the insect body does not move, it is dead.

[0129] Mortality rate (%) = 100 × number of dead insects / number of test insects.

[0130] Corrected mortality rate (%) = 100 × (mortality rate of treatment group - mortality rate of control group) / (1 - mortality rate of control group).

[0131] LD50 50 ) is the dose that causes 50% mortality of entomophilous book lice, and the median lethal dose was calculated using the Probit software package.

[0132] The results are shown in Table 4:

[0133] Table 4. Contact toxicity of the essential oil of scutellaria baicalensis to the entomophilous book lice determined by the drug film method

[0134]

[0135]

[0136] The contact activity of Atractylodes lanceolatus against adults of the entomophilous book lice was LD50. 50 =157.41μg / cm 2 , the 95% confidence interval is (111.36, 189.63), y = 2.34x-5.13. The half lethal dose means that each square centimeter contains 157.41 μg of essential oil. At this dose, half of the test subjects died.

[0137] Experimental Example 6: Determination of the repellent activity of the essential oil of Rhizoma Cibotii against stored-warehouse pests

[0138] The method for determining the repellent activity of the essential oil of the scutellaria baicalensis against the lice is as follows: select a glass culture dish of the same size (diameter 5.8 cm), evenly apply a circle of polytetrafluoroethylene on the inner wall, blow dry with a hair dryer to form a film of uniform thickness and smoothness; cut the filter paper into a circular paper piece (diameter 5.8 cm) of the same size as the inner diameter of the culture dish, and cut it into two halves of the same size from the middle; dilute the volatile oil of the scutellaria baicalensis with acetone to prepare a gradient concentration (8 μg / cm 2 , 4μg / cm 2 , 2μg / cm 2 and 1 μg / cm 2 ), use a pipette to draw 150μL of the drug solution and evenly drop it on half of the filter paper, and add 150μL of acetone to the other half of the filter paper as a blank control group, and DEET as a positive control; let it stand for about one minute, and then stick the filter paper to the bottom of the culture dish after the solvent evaporates; pick 20 adult insects in the middle of the culture dish, observe and record the number of test insects in the experimental group and the blank control group after 2h, use a brush to gently move the test insects to the middle of the culture dish, observe and record the distribution of the test insects again after 4h, and calculate the repellency rate.

[0139] Repellency rate PR (%) = (number of insects in the control group - number of insects in the experimental group) / total number of test insects × 100%.

[0140] Table 5 Standards for Classification of the Repellent Activity of the Essential Oil of Clove

[0141] Table 5. Classification criteria for repellent activity of essential oils

[0142]

[0143] Table 6. Repellent activity of the essential oil of scutellaria baicalensis against entomophilous book lice

[0144]

[0145] The essential oil of scutellaria baicalensis has a strong repellent activity against insectivorous book lice. 2 The repellency rates at 2 hours and 4 hours were comparable to those of DEET, both of which were at the strongest level V; at a concentration of 4.0 μg / cm 2 The repellency rate of 2 hours to insectivorous book lice was the strongest level V, and the repellency rate of 4 hours was level IV; at the lowest concentration tested, 1.0 μg / cm 2 The repellency rate against insectivorous book lice reached level III after 2 hours and still reached level III after 4 hours.

Claims

1. The biological control use of the sticky hairy fragrant green, characterized in that, The biological control refers to the use of the essential oil of the scutellaria baicalensis to control pests or mites; the essential oil of the scutellaria baicalensis is an essential oil obtained by extracting an oil-water mixture obtained by boiling the aerial parts of the scutellaria baicalensis plant; the pests are western flower thrips and entomophilous book lice; the mites are two-spotted spider mites; before extraction, the oil-water mixture is condensed, recovered and then extracted; the extraction agent is n-hexane.

2. The use of the scutellaria baicalensis for preparing a biocontrol agent, characterized in that: The essential oil of Herba Lysimachiae is used as the active ingredient of the biological control preparation; the essential oil of Herba Lysimachiae is the essential oil obtained by extracting the oil-water mixture obtained by boiling the aerial part of the Herba Lysimachiae plant; the biological control refers to the use of the essential oil of Herba Lysimachiae to control pests or mites; the pests are western flower thrips and entomophilous book lice; the mites are spider mites two-spotted; before extraction, the oil-water mixture is condensed, recovered and then extracted; the extraction agent is n-hexane.

3. A biocontrol agent comprising: The active ingredient is characterized in that the active ingredient includes the essential oil of Scutellaria baicalensis; the essential oil of Scutellaria baicalensis is an essential oil obtained by extracting the oil-water mixture obtained by boiling the aerial part of the Scutellaria baicalensis plant; before the extraction, the oil-water mixture is condensed, recovered and then extracted; the extraction agent of the extraction is n-hexane.

4. A biocontrol agent according to claim 3, characterized in that: Also includes: Auxiliary materials.

5. A method for preparing a biocontrol agent, characterized in that: include: The essential oil of Psoralea corylifolia is used as the active ingredient of biocontrol agents; The linalool essential oil is an essential oil obtained by extracting an oil-water mixture obtained by boiling the aerial parts of the linalool plant in water; before the extraction, the oil-water mixture is condensed, recovered and then extracted; the extraction agent is n-hexane.

6. The method for preparing a biocontrol agent according to claim 5, characterized in that: The distillation time of the water boiling method is 6 hours.

7. The method for preparing a biocontrol agent according to claim 5, characterized in that: The extraction temperature was 30°C.

8. A method for preparing a biocontrol agent according to any one of claims 5 to 7, characterized in that: After extraction, dry.

9. The method for preparing a biocontrol agent according to claim 8, characterized in that: The drying refers to dehydration by adding anhydrous sodium sulfate.

10. The method for preparing a biocontrol agent according to claim 9, characterized in that: After drying, the extract was evaporated and concentrated at 40°C using a rotary evaporator to remove the extractant.

11. A method for preparing a biocontrol agent according to any one of claims 5-7, 9-10, characterized in that: The method for preparing the biocontrol agent further comprises: adding auxiliary materials.

12. A biological control method, characterized in that: The biological control refers to the use of the essential oil of the scutellaria baicalensis to control pests or mites; the essential oil of the scutellaria baicalensis is an essential oil obtained by extracting an oil-water mixture obtained by boiling the aerial parts of the scutellaria baicalensis plant; before the extraction, the oil-water mixture is condensed, recovered and then extracted; the extraction agent is n-hexane; the pests are western flower thrips and entomophilous book lice; the mites are two-spotted spider mites.

13. A biological control method according to claim 12, characterized in that: The control refers to killing pests or mites by fumigation or contact with the essential oil of Scutellaria baicalensis, or repelling pests with the essential oil of Scutellaria baicalensis.

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