A feed additive containing plant polysaccharides and its preparation method and application

Abstract

The present invention relates to a feed additive containing plant polysaccharides, a preparation method and an application thereof, and belongs to the technical field of feed additives. The present invention discloses a feed additive containing plant polysaccharides, which includes the following raw materials in parts by weight: 50-70 parts of composite plant polysaccharides, 10-15 parts of kelp powder, 5-10 parts of soy isoflavones, and 10-15 parts of spirulina powder. Polysaccharides can promote the activity of probiotics, activate antioxidant capacity, and help release free glycerol by inducing lipolysis of fat cells, reduce lipid accumulation, and inhibit lipase activity to significantly reduce glycerol absorption in the intestine; kelp powder and soy isoflavones promote the synthesis of thyroid hormones and improve the vitality of animals. The feed additive containing plant polysaccharides prepared by the present invention can be applied to fish feed, poultry feed and livestock feed.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives and relates to a feed additive containing plant polysaccharides and a preparation method and application thereof. Background Art

[0002] Farming modes are divided into traditional farming and intensive farming. Due to site restrictions and more convenient management, intensive farming has become the main form of farming scale. Intensive farming can improve the production efficiency of poultry. Through scientific feeding management and environmental control, the growth and weight gain of poultry can be optimized, the feed conversion rate can be improved, and the waste of resources can be reduced, thereby improving the economic benefits of farming. However, the activities of animals in intensive farming are restricted, and there are a lot of nutrients in the feed. The excess nutrients will be converted into fat and accumulated in the animal's body, which will affect health and seriously affect the taste.

[0003] Polysaccharides are a class of natural macromolecular polymers connected by glycosidic bonds between aldose or ketose. Polysaccharides are widely available in nature, especially in higher plants, animals, microorganisms, and seaweed. Polysaccharides play an extremely important and decisive role in cell recognition, controlling cell division and differentiation. Active polysaccharides can directly activate immune cells, enhance the phagocytosis and killing effects of animal immune cells, activate the complement system, promote the production of antibodies, and the synthesis and secretion of cytokines. Summary of the invention

[0004] The present invention relates to a feed additive containing plant polysaccharides, a preparation method and application thereof, and belongs to the technical field of feed additives. The present invention discloses a feed additive containing plant polysaccharides, which includes the following raw materials in parts by weight: 50-70 parts of composite plant polysaccharides, 10-15 parts of kelp powder, 5-10 parts of soy isoflavones, and 10-15 parts of spirulina powder. Polysaccharides can promote the activity of probiotics and activate antioxidant capacity. In addition, they can help release free glycerol by inducing lipolysis of fat cells, reduce lipid accumulation, and inhibit lipase activity to significantly reduce the absorption of glycerol in the intestine; kelp powder and soy isoflavones promote the synthesis of thyroid hormones and improve the vitality of animals. The feed additive containing plant polysaccharides prepared by the present invention can be applied to fish feed, poultry feed and livestock feed.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 50-70 parts of composite plant polysaccharides, 10-15 parts of kelp powder, 5-10 parts of soybean isoflavones and 10-15 parts of spirulina powder.

[0007] Furthermore, the preparation method of the composite plant polysaccharide comprises the following steps:

[0008] A: Longan is shelled and pitted, then dried in the sun, ground into longan powder, soaked in ethanol solution, and then centrifuged to obtain a mixed solution a;

[0009] B: Add distilled water to the mixed solution a, heat and stir, let stand and then filter to obtain the mixed solution b;

[0010] C: removing the core of the red dates, soaking them in anhydrous ethanol, and then drying them to obtain the red dates preparation;

[0011] D: crushing the prepared red dates into red date powder, adding a solvent to form a mixture, pressurizing for ultrasonic treatment, and filtering to obtain a mixed solution c;

[0012] E: Mix the mixed solution b and the mixed solution c, and then heat them to evaporate and concentrate to obtain the composite plant polysaccharide.

[0013] Furthermore, the mass fraction of the ethanol solution in step A is 80%, the solid-liquid ratio of the longan powder and the ethanol solution is 1:5 g / mL, the soaking time is 16-24 h, and the speed and time of the centrifugal separation are 4000 r / min and 5-10 min, respectively.

[0014] Furthermore, in step B, the volume ratio of the mixed solution a to distilled water is 1:15-20, the heating refers to heating to 80° C., and the standing time is 2-3 h.

[0015] Furthermore, in the step C, the solid-liquid ratio of red dates to anhydrous ethanol is 1:10 g / mL, the soaking time in anhydrous ethanol is 30-60 min, and the drying temperature is 40-50°C.

[0016] Furthermore, in the step D, the solid-liquid ratio of the red date powder to the solvent is 1:5 g / mL, the solvent is composed of choline chloride and ethylene glycol in a mass ratio of 1:1, the pressurization pressure is 20-30 MPa, and the ultrasonic time is 20-30 min.

[0017] Furthermore, in the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the heating refers to heating to 50-60°C.

[0018] Furthermore, the preparation method of the feed additive containing plant polysaccharides is: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

[0019] An application of the feed additive containing plant polysaccharides, wherein the feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

[0020] Beneficial effects of the present invention:

[0021] 1. The plant polysaccharide prepared by combining longan and red dates enhances the activity of probiotics, and helps release free glycerol by inducing lipolysis of adipocytes, reduces lipid accumulation, and inhibits lipase activity to significantly reduce intestinal absorption of glycerol. The feed additive containing plant polysaccharides prepared by the present invention can effectively reduce the fat content in animals.

[0022] 2. Adding soy isoflavones and polysaccharides in combination can affect the expression of related proteins and genes through various signal pathways, and affect the biosynthesis of fatty acids, the biosynthesis of steroid hormones, the biosynthesis of unsaturated fatty acids, fatty acid elongation, glycerolipid metabolism and glycerophospholipid metabolism, thereby further preventing the possibility of nutrients being converted into fat.

[0023] 3. Kelp powder and spirulina powder are rich in iodine, vitamins, amino acids and other substances, which promote the synthesis of animal thyroid hormones, make animals more energetic, reduce fat accumulation through exercise and make the meat taste better. DETAILED DESCRIPTION

[0024] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with embodiments.

[0025] Example 1

[0026] A feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 50 parts of composite plant polysaccharides, 10 parts of kelp powder, 5 parts of soybean isoflavones and 10 parts of spirulina powder.

[0027] The preparation method of the composite plant polysaccharide comprises the following steps:

[0028] A: Longan is shelled and pitted, then dried in the sun, ground into longan powder, soaked in ethanol solution, and then centrifuged to obtain a mixed solution a;

[0029] B: Add distilled water to the mixed solution a, heat and stir, let stand and then filter to obtain the mixed solution b;

[0030] C: removing the core of the red dates, soaking them in anhydrous ethanol, and then drying them to obtain the red dates preparation;

[0031] D: crushing the prepared red dates into red date powder, adding a solvent to form a mixture, pressurizing for ultrasonic treatment, and filtering to obtain a mixed solution c;

[0032] E: Mix the mixed solution b and the mixed solution c, and then heat them to evaporate and concentrate to obtain the composite plant polysaccharide.

[0033] The mass fraction of the ethanol solution in step A is 80%, the solid-liquid ratio of the longan powder and the ethanol solution is 1:5 g / mL, the soaking time is 16 hours, and the speed and time of the centrifugal separation are 4000 r / min and 5 minutes respectively.

[0034] In the step B, the volume ratio of the mixed solution a to distilled water is 1:15, the heating refers to heating to 80° C., and the standing time is 2 hours.

[0035] In the step C, the solid-liquid ratio of red dates to anhydrous ethanol is 1:10 g / mL, the anhydrous ethanol soaking time is 30 min, and the drying temperature is 40° C.

[0036] In the step D, the solid-liquid ratio of the red date powder to the solvent is 1:5 g / mL, the solvent is composed of choline chloride and ethylene glycol in a mass ratio of 1:1, the pressurization pressure is 20 MPa, and the ultrasonic time is 20 min.

[0037] In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the heating refers to heating to 50°C.

[0038] The preparation method of the feed additive containing plant polysaccharides is as follows: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

[0039] An application of the feed additive containing plant polysaccharides, wherein the feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

[0040] Example 2

[0041] A feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 60 parts of composite plant polysaccharides, 10 parts of kelp powder, 5 parts of soybean isoflavones and 10 parts of spirulina powder.

[0042] The preparation method of the composite plant polysaccharide comprises the following steps:

[0043] A: Longan is shelled and pitted, then dried in the sun, ground into longan powder, soaked in ethanol solution, and then centrifuged to obtain a mixed solution a;

[0044] B: Add distilled water to the mixed solution a, heat and stir, let stand and then filter to obtain the mixed solution b;

[0045] C: removing the core of the red dates, soaking them in anhydrous ethanol, and then drying them to obtain the red dates preparation;

[0046] D: crushing the prepared red dates into red date powder, adding a solvent to form a mixture, pressurizing for ultrasonic treatment, and filtering to obtain a mixed solution c;

[0047] E: Mix the mixed solution b and the mixed solution c, and then heat them to evaporate and concentrate to obtain the composite plant polysaccharide.

[0048] The mass fraction of the ethanol solution in step A is 80%, the solid-liquid ratio of the longan powder and the ethanol solution is 1:5 g / mL, the soaking time is 20 hours, and the speed and time of the centrifugal separation are 4000 r / min and 10 min respectively.

[0049] In the step B, the volume ratio of the mixed solution a to distilled water is 1:18, the heating refers to heating to 80° C., and the standing time is 2 hours.

[0050] In the step C, the solid-liquid ratio of red dates to anhydrous ethanol is 1:10 g / mL, the anhydrous ethanol soaking time is 45 min, and the drying temperature is 40° C.

[0051] In the step D, the solid-liquid ratio of the red date powder to the solvent is 1:5 g / mL, the solvent is composed of choline chloride and ethylene glycol in a mass ratio of 1:1, the pressurization pressure is 25 MPa, and the ultrasonic time is 25 min.

[0052] In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the heating refers to heating to 55°C.

[0053] The preparation method of the feed additive containing plant polysaccharides is as follows: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

[0054] An application of the feed additive containing plant polysaccharides, wherein the feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

[0055] Example 3

[0056] A feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 70 parts of composite plant polysaccharides, 15 parts of kelp powder, 10 parts of soybean isoflavones and 15 parts of spirulina powder.

[0057] The preparation method of the composite plant polysaccharide comprises the following steps:

[0058] A: Longan is shelled and pitted, then dried in the sun, ground into longan powder, soaked in ethanol solution, and then centrifuged to obtain a mixed solution a;

[0059] B: Add distilled water to the mixed solution a, heat and stir, let stand and then filter to obtain the mixed solution b;

[0060] C: removing the core of the red dates, soaking them in anhydrous ethanol, and then drying them to obtain the red dates preparation;

[0061] D: crushing the prepared red dates into red date powder, adding a solvent to form a mixture, pressurizing for ultrasonic treatment, and filtering to obtain a mixed solution c;

[0062] E: Mix the mixed solution b and the mixed solution c, and then heat them to evaporate and concentrate to obtain the composite plant polysaccharide.

[0063] The mass fraction of the ethanol solution in step A is 80%, the solid-liquid ratio of the longan powder and the ethanol solution is 1:5 g / mL, the soaking time is 24 hours, and the speed and time of the centrifugal separation are 4000 r / min and 10 minutes respectively.

[0064] In the step B, the volume ratio of the mixed solution a to distilled water is 1:20, the heating refers to heating to 80° C., and the standing time is 3 hours.

[0065] In the step C, the solid-liquid ratio of red dates to anhydrous ethanol is 1:10 g / mL, the anhydrous ethanol soaking time is 60 min, and the drying temperature is 50° C.

[0066] In the step D, the solid-liquid ratio of the red date powder to the solvent is 1:5 g / mL, the solvent is composed of choline chloride and ethylene glycol in a mass ratio of 1:1, the pressurization pressure is 30 MPa, and the ultrasonic time is 30 min.

[0067] In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the heating refers to heating to 60°C.

[0068] The preparation method of the feed additive containing plant polysaccharides is as follows: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

[0069] An application of the feed additive containing plant polysaccharides, wherein the feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

[0070] Comparative Example 1

[0071] On the basis of Example 2, a feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 60 parts of plant polysaccharides, 10 parts of kelp powder, 5 parts of soy isoflavones, and 10 parts of spirulina powder.

[0072] The method for preparing the plant polysaccharide comprises the following steps:

[0073] A: Remove the core of the red dates, soak them in anhydrous ethanol, and then dry them to obtain the red dates preparation;

[0074] B: crushing the prepared red dates into red date powder, adding a solvent to form a mixture, pressurizing for ultrasonic treatment, and filtering to obtain a mixed solution c;

[0075] C: Mixed liquid c is heated and evaporated to concentrate to obtain plant polysaccharides.

[0076] In step A, the solid-liquid ratio of red dates to anhydrous ethanol is 1:10 g / mL, the anhydrous ethanol soaking time is 45 min, and the drying temperature is 40° C.

[0077] In the step B, the solid-liquid ratio of the red date powder to the solvent is 1:5 g / mL, the solvent is composed of choline chloride and ethylene glycol in a mass ratio of 1:1, the pressurization pressure is 25 MPa, and the ultrasonic time is 25 min.

[0078] In step C, the heating refers to heating to 55°C.

[0079] The preparation method of the feed additive containing plant polysaccharides is as follows: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

[0080] An application of the feed additive containing plant polysaccharides, wherein the feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

[0081] Comparative Example 2

[0082] On the basis of Example 2, a feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 60 parts of plant polysaccharides, 10 parts of kelp powder, 5 parts of soy isoflavones, and 10 parts of spirulina powder.

[0083] The method for preparing the plant polysaccharide comprises the following steps:

[0084] A: Longan is shelled and pitted, then dried in the sun, ground into longan powder, soaked in ethanol solution, and then centrifuged to obtain a mixed solution a;

[0085] B: Add distilled water to the mixed solution a, heat and stir, let stand and then filter to obtain the mixed solution b;

[0086] C: Heat the mixed solution b to evaporate and concentrate to obtain plant polysaccharides.

[0087] The mass fraction of the ethanol solution in step A is 80%, the solid-liquid ratio of the longan powder and the ethanol solution is 1:5 g / mL, the soaking time is 20 hours, and the speed and time of the centrifugal separation are 4000 r / min and 10 min respectively.

[0088] In the step B, the volume ratio of the mixed solution a to distilled water is 1:18, the heating refers to heating to 80° C., and the standing time is 2 hours.

[0089] In step C, the heating refers to heating to 55°C.

[0090] The preparation method of the feed additive containing plant polysaccharides is as follows: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

[0091] An application of the feed additive containing plant polysaccharides, wherein the feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

[0092] Comparative Example 3

[0093] On the basis of Example 2, the soy isoflavones in the raw material components were removed, and the other conditions were the same as those in Example 2.

[0094] Comparative Example 4

[0095] On the basis of Example 2, the kelp powder in the raw material components was removed and replaced with an equal weight portion of spirulina powder, and other conditions were consistent with Example 2.

[0096] Comparative Example 5

[0097] On the basis of Example 2, the spirulina powder in the raw material components was removed and replaced with kelp powder in equal weight portions. Other conditions were the same as in Example 2.

[0098] 1. Performance Testing

[0099] The feed additive containing plant polysaccharides prepared in Examples 1-3 and Comparative Examples 1-5 was added to poultry feed as a sample, and the addition amount was 10%. The poultry feed consisted of the following raw materials by weight: 2.0 parts of corn, 1.3 parts of sorghum, 1.7 parts of barley, 2.0 parts of leaf bran, 0.5 parts of bean cake and soybean meal, 1 part of yam powder, and 1.5 parts of wheat bran. 800 healthy chicks born 30 days ago were divided into 8 groups, raised in a pen and fed with the sample, and the specific feeding method was: once in the morning, noon, and evening. In addition, 100 chicks were selected and fed with feed without additives as a control group.

[0100] Fat test: Use ultrasonic instrument to scan the belly of chicken to determine the fat content, measure it once a month, test for 3 months respectively, and calculate the fat increment. The above test results are shown in Table 1.

[0101] Table 1 Test results

[0102]

[0103]

[0104] As can be seen from Table 1, the monthly fat increment of Examples 1-3 is less than that of Comparative Examples 1-5 and the control group, among which the monthly fat increment of the control group is the largest. Comparative Example 1 does not add longan compound; Comparative Example 2 does not add red dates compound; Comparative Example 3 lacks the addition of soy isoflavones; Comparative Examples 4-5 only add one of kelp powder and spirulina powder. Therefore, the lack of any one component affects the fat content.

[0105] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A feed additive containing plant polysaccharides, characterized in that: The feed additive containing plant polysaccharides comprises the following raw materials in parts by weight: 50-70 parts of composite plant polysaccharides, 10-15 parts of kelp powder, 5-10 parts of soybean isoflavones, and 10-15 parts of spirulina powder; The preparation method of the composite plant polysaccharide comprises the following steps: A: Longan is shelled and pitted, then dried in the sun, ground into longan powder, soaked in an ethanol solution, and then centrifuged to obtain a mixed solution a, wherein the solid-to-liquid ratio of the longan powder to the ethanol solution is 1:5 g / mL; B: Add distilled water to the mixed solution a, heat and stir, let stand and filter to obtain the mixed solution b; the volume ratio of the mixed solution a to distilled water is 1:15-20; C: removing the core of the red dates, soaking them in anhydrous ethanol, and then drying them to obtain the red dates preparation; D: crushing the red date material into red date powder, adding a solvent to form a mixture, pressurizing for ultrasonic treatment, filtering to obtain a mixed solution c; the solid-liquid ratio of the red date powder to the solvent is 1:5 g / mL; the solvent is composed of choline chloride and ethylene glycol in a mass ratio of 1:1; E: Mixing the mixed solution b and the mixed solution c, and then heating and evaporating to concentrate to obtain the composite plant polysaccharide; The preparation method of the feed additive containing plant polysaccharides is as follows: all raw material components are mixed to obtain the feed additive containing plant polysaccharides.

2. A feed additive containing plant polysaccharides according to claim 1, characterized in that: The mass fraction of the ethanol solution in step A is 80%, the soaking time is 16-24h, and the speed and time of the centrifugal separation are 4000 r / min and 5-10min respectively.

3. A feed additive containing plant polysaccharides according to claim 1, characterized in that: In the step B, the heating is heating to 80° C., and the standing time is 2-3 hours.

4. A feed additive containing plant polysaccharides according to claim 1, characterized in that: In the step C, the solid-liquid ratio of red dates to anhydrous ethanol is 1:10 g / mL, the soaking time in anhydrous ethanol is 30-60 min, and the drying temperature is 40-50° C.

5. The feed additive containing plant polysaccharides according to claim 1, characterized in that: In the step D, the pressurization pressure is 20-30 MPa, and the ultrasonication time is 20-30 min.

6. A feed additive containing plant polysaccharides according to claim 1, characterized in that: In the step E, the volume ratio of the mixed solution b to the mixed solution c is 1:1, and the heating refers to heating to 50-60°C.

7. A use of a feed additive containing plant polysaccharides according to any one of claims 1 to 6, characterized in that: The feed additive containing plant polysaccharides is applied to fish feed, poultry feed and livestock feed.

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