Corn-unconventional protein source diet for growing pigs and feeding method thereof

Abstract

Corn-unconventional protein source growing pig diet and feeding method thereof, relating to an improved unconventional protein source feed and feeding method thereof, belonging to the technical field of growing pig feed preparation. The invention is to solve the problem of soybean meal shortage. The corn-unconventional protein source growing pig diet is as follows: it consists of 55.0-65.0 parts of corn, 1.0-5.0 parts of wheat bran, 15.0-20.0 parts of soybean meal, 1.5-3.0 parts of rapeseed meal, 1.5-3.0 parts of cottonseed meal, 6.0-10.0 parts of distiller's grains protein feed (DDGS), 1.0-3.0 parts of sucrose, 1.0-3.0 parts of soybean oil, 0.1-0.5 parts of lysine hydrochloride, 0.01-0.05 parts of methionine, 0.05-0.1 parts of cysteine, 0.05-0.1 parts of threonine, 1.0-1.5 parts of stone powder, 1.0-1.5 parts of calcium hydrogen phosphate, 0.5-1.0 parts of sodium chloride and 1.0 parts of premix; the feeding method of the corn-unconventional protein source growing pig diet is feeding 6 times a day. Unconventional protein sources account for 20.0%-25.0% of total protein, reducing soybean meal by 5.0%-8.0%.

Description

Technical Field

[0001] The invention relates to an improved unconventional protein source feed and a feeding method thereof, and belongs to the field of growing pig feed preparation technology and feeding management methods. Background Art

[0002] Corn-unconventional protein source feeds are not as well-balanced and balanced in amino acid release as corn-soybean meal feeds, so their feed utilization rate is not as good as the latter. Feeding mode is one of the important factors affecting the growth performance of growing and finishing pigs. At present, in most commercial pig production in my country, there is no industry standard for feeding frequency. During the growth and fattening stage, most of them are fed in a free-feeding mode. Exploring the appropriate feeding frequency can improve feed utilization and alleviate feed waste to a certain extent, which is of great significance to pig production. Summary of the invention

[0003] The purpose of the invention is to solve the technical problem of soybean meal shortage and provide a corn-unconventional protein source growing pig diet and a feeding method thereof.

[0004] The corn-unconventional protein source growing pig diet is as follows: by weight, it consists of 55.0-65.0 parts of corn, 1.0-5.0 parts of wheat bran, 15.0-20.0 parts of soybean meal, 1.5-3.0 parts of rapeseed meal, 1.5-3.0 parts of cottonseed meal, 6.0-10.0 parts of dry distillers grains from corn (DDGS), 1.0-3.0 parts of sucrose, 1.0-3.0 parts of soybean oil, 0.1-0.5 parts of lysine hydrochloride, 0.01-0.05 parts of methionine, 0.05-0.1 parts of cysteine, 0.04-0.1 parts of threonine, 1.0-1.5 parts of stone powder, 1.0-1.5 parts of calcium hydrogen phosphate, 0.5-1.0 parts of sodium chloride and 1.0 parts of premix;

[0005] The premix consists of vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, calcium pantothenate, antioxidant, choline chloride, CoSO4, CuSO4·5H2O, FeSO4·H2O, MnO, ZnSO4, KI and Na2SeO3, and each kilogram of corn-unconventional protein source growing pig diet contains 28,500 IU of vitamin A, 36,000 IU of vitamin D, 67.5 IU of vitamin E, 37.5 mg of vitamin K, 17.5 mg of vitamin B1, 215 mg of vitamin B2, 69 mg of vitamin B6, 0.075 mg of vitamin B12, 70 mg of niacin, 3 mg of folic acid, 0.375 mg of calcium pantothenate, 0.15 mg of antioxidant, 105 mg of choline chloride, 1 mg of CoSO4, 1 mg of CuSO4·5H2O, 155mg, FeSO4·H2O 145mg, MnO75mg, ZnSO4 125mg, KI 0.3mg, Na2SeO3 0.3mg.

[0006] The feeding method of the corn-unconventional protein source growing pig diet is to feed 6 times a day.

[0007] The effects achieved by the present invention are:

[0008] Corn-unconventional protein source growing pig diet includes basic diet and premix, with unconventional protein source accounting for 20.0%-25.0% of total protein and reducing soybean meal by 5.0%-8.0%. According to the current price of feed raw materials, unconventional protein source feed reduces the cost per ton of feed by 2.0%-4.0% compared with soybean meal feed. P <0.05), which increased feed compensation.

[0009] The present invention improves the growth performance of unconventional protein source feeds by increasing the feeding frequency, and alleviates the gap in growth performance between the unconventional protein source feeds and the soybean meal feeds. It can reduce the manure nitrogen (FN), urine nitrogen (UN), and total nitrogen emission (TN) of growing pigs; improve the digestibility (AND), utilization rate (NPU), and biological value (ABV) of nitrogen in feed for growing pigs. The total nitrogen emission is reduced by 2.0%-3.0%, and the AND, NPU, and ABV of nitrogen in feed for growing pigs are 3.0%-7.0%. The utilization efficiency of nitrogen in unconventional protein source feeds is improved, and the gap in nitrogen utilization with soybean meal feeds is alleviated. Unconventional protein source feeds are compared with soybean meal feeds. The feed cost per ton is reduced by 2.0%-4.0% (P<0.05), and the feed reward is improved. If corn-unconventional protein source feeds can be promoted and applied, the problem of soybean meal shortage can be solved and feeding costs can be saved. DETAILED DESCRIPTION

[0010] The technical solution of the present invention is not limited to the specific implementation modes listed below, but also includes any combination of the specific implementation modes.

[0011] Specific implementation method one: In this implementation method, the corn-unconventional protein source growing pig diet is as follows: by weight, it consists of 55.0-65.0 parts of corn, 1.0-5.0 parts of wheat bran, 15.0-20.0 parts of soybean meal, 1.5-3.0 parts of rapeseed meal, 1.5-3.0 parts of cottonseed meal, 6.0-10.0 parts of dry corn alcohol grains, 1.0-3.0 parts of sucrose, 1.0-3.0 parts of soybean oil, 0.1-0.5 parts of lysine hydrochloride, 0.01-0.05 parts of methionine, 0.05-0.1 parts of cysteine, 0.04-0.1 parts of threonine, 1.0-1.5 parts of stone powder, 1.0-1.5 parts of calcium hydrogen phosphate, 0.5-1.0 parts of sodium chloride and 1.0 parts of premix;

[0012] The premix consists of vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, calcium pantothenate, antioxidant, choline chloride, CoSO4, CuSO4·5H2O, FeSO4·H2O, MnO, ZnSO4, KI and Na2SeO3, and each kilogram of corn-unconventional protein source growing pig diet contains 28,500 IU of vitamin A, 36,000 IU of vitamin D, 67.5 IU of vitamin E, 37.5 mg of vitamin K, 17.5 mg of vitamin B1, 215 mg of vitamin B2, 69 mg of vitamin B6, 0.075 mg of vitamin B12, 70 mg of niacin, 3 mg of folic acid, 0.375 mg of calcium pantothenate, 0.15 mg of antioxidant, 105 mg of choline chloride, 1 mg of CoSO4, 1 mg of CuSO4·5H2O, 155mg, FeSO4·H2O 145mg, MnO75mg, ZnSO4 125mg, KI 0.3mg, Na2SeO3 0.3mg.

[0013] Specific embodiment 2: This embodiment is different from specific embodiment 1 in that the corn-unconventional protein source growing pig diet is as follows: composed, by weight, of 60.12 parts of corn, 2.00 parts of wheat bran, 16.86 parts of soybean meal, 2.57 parts of rapeseed meal, 2.50 parts of cottonseed meal, 7.50 parts of dry corn distillers grains, 1.35 parts of sucrose, 2.79 parts of soybean oil, 0.32 parts of lysine hydrochloride, 0.03 parts of methionine, 0.08 parts of cysteine, 0.04 parts of threonine, 1.04 parts of stone powder, 1.00 parts of calcium hydrogen phosphate, 0.8 parts of sodium chloride and 1.0 parts of premix; the rest is the same as specific embodiment 1.

[0014] Specific implementation method three: The feeding method of the corn-unconventional protein source diet for growing pigs described in one of specific implementation methods one to two is to feed 6 times a day.

[0015] The following test is used to verify the effect of the present invention:

[0016] Test 1:

[0017] The corn-unconventional protein source growing pig diet (MLM) is as follows: The corn-unconventional protein source growing pig diet is as follows: It consists of 60.12 parts of corn, 2.00 parts of wheat bran, 16.86 parts of soybean meal, 2.57 parts of rapeseed meal, 2.50 parts of cottonseed meal, 7.50 parts of dry corn distillers grains, 1.35 parts of sucrose, 2.79 parts of soybean oil, 0.32 parts of lysine hydrochloride, 0.03 parts of methionine, 0.08 parts of cysteine, 0.04 parts of threonine, 1.04 parts of stone powder, 1.00 parts of calcium hydrogen phosphate, 0.8 parts of sodium chloride and 1.0 parts of premix in parts by weight;

[0018] The premix consists of vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, calcium pantothenate, antioxidant, choline chloride, CoSO4, CuSO4·5H2O, FeSO4·H2O, MnO, ZnSO4, KI and Na2SeO3, and each kilogram of corn-unconventional protein source growing pig diet contains 28,500 IU of vitamin A, 36,000 IU of vitamin D, 67.5 IU of vitamin E, 37.5 mg of vitamin K, 17.5 mg of vitamin B1, 215 mg of vitamin B2, 69 mg of vitamin B6, 0.075 mg of vitamin B12, 70 mg of niacin, 3 mg of folic acid, 0.375 mg of calcium pantothenate, 0.15 mg of antioxidant, 105 mg of choline chloride, 1 mg of CoSO4, 1 mg of CuSO4·5H2O, 155mg, FeSO4·H2O 145mg, MnO75mg, ZnSO4 125mg, KI 0.3mg, Na2SeO3 0.3mg.

[0019] Corn-soybean meal (SBM) growing pig diet was used as the experimental control group. The corn-soybean meal growing pig diet consisted of 61.45 parts of corn, 7.50 parts of wheat bran, 22.59 parts of soybean meal, 1.31 parts of sucrose, 2.79 parts of soybean oil, 0.24 parts of lysine hydrochloride, 0.04 parts of methionine, 0.07 parts of cysteine, 0.05 parts of threonine, 1.06 parts of stone powder, 1.07 parts of calcium hydrogen phosphate, 0.83 parts of sodium chloride and 1.0 parts of premix by weight.

[0020] The premix consists of vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, calcium pantothenate, antioxidant, choline chloride, CoSO4, CuSO4·5H2O, FeSO4·H2O, MnO, ZnSO4, KI and Na2SeO3, and each kilogram of corn-unconventional protein source growing pig diet contains 28,500 IU of vitamin A, 36,000 IU of vitamin D, 67.5 IU of vitamin E, 37.5 mg of vitamin K, 17.5 mg of vitamin B1, 215 mg of vitamin B2, 69 mg of vitamin B6, 0.075 mg of vitamin B12, 70 mg of niacin, 3 mg of folic acid, 0.375 mg of calcium pantothenate, 0.15 mg of antioxidant, 105 mg of choline chloride, 1 mg of CoSO4, 1 mg of CuSO4·5H2O, 155mg, FeSO4·H2O 145mg, MnO75mg, ZnSO4 125mg, KI 0.3mg, Na2SeO3 0.3mg.

[0021] Specific test steps and data results:

[0022] 1 Materials and methods

[0023] 1.1 Test materials

[0024] Experimental animals (pigs); weighing scales; reagents: concentrated sulfuric acid; copper sulfate; boric acid; dilute hydrochloric acid; methyl red; bromocresol green; sodium hydroxide; petroleum ether; instruments: vernier caliper; tape measure; sulfuric acid paper; filter paper; weighing bottle; analytical balance; fully automatic Kjeldahl nitrogen analyzer (FOSS); fully automatic biochemical analyzer (Biobase); oven.

[0025] 1.2 Experimental design

[0026] This experiment took two different feeding frequencies and two different dietary protein sources as the main influencing factors, and designed each factor and treatment method according to the 2×2 factor treatment scheme, namely, the corn-soybean meal diet group fed twice a day (T.SBM); the corn-soybean meal diet group fed 6 times a day (S.SBM); the corn-unconventional protein source growing pig diet group fed twice a day (T.MLM); the corn-unconventional protein source growing pig diet group fed 6 times a day (S.MLM). 24 Duroc×Chang×Da three-way crossbred boars were selected for the experiment, with an initial weight of 27.9±3.6kg and an average age of 75 days. The experimental period was 6 weeks, including a pre-feeding period of 7 days, a growth performance test of 4 weeks, and a metabolic test of 1 week.

[0027] 1.3 Feeding and management

[0028] The experiment was conducted at the livestock and poultry farm of Jilin Agricultural University. The pig house has good insulation and ventilation capabilities. Before the experiment, the pens were cleaned and washed, and fumigated with formaldehyde and potassium permanganate for 24 hours. During the experiment, epidemic prevention and disinfection were carried out every two days, and the disinfectant used was Virkon (potassium persulfate complex) and peracetic acid alternately. The temperature in the shed was maintained at 20-23℃ during the test period. The fence was equipped with an automatic water supply device, an electronic scale, and feces and urine collection trays. The experimental pigs were randomly assigned to 24 pig pens, with one pig in each pig pen, and fed two kinds of diets (SBM diet; MLM diet) at two feeding frequencies (2 times; 6 times) to form four treatment groups (n = 6 for each treatment). After the growth performance test was completed, the pigs were transferred to metabolic cages for metabolic tests.

[0029] 1.4 Feeding method

[0030] The SBM2 and MLM2 groups were fed twice a day at 7:00 and 17:00, with one-half of the feeding standard each time, and the remaining feed after each pig was eaten was taken out at 9:30 and 19:30, weighed and recorded; the SBM6 and MLM6 groups were fed at 7:00, 9:30, 12:00, 14:30, 17:00, and 19:30 every day, with one-sixth of the feeding standard each time, and the remaining feed after each pig was eaten was taken out at 21:00, weighed and recorded. Growing pigs drink water freely from duckbill drinkers. The adaptation period was 7 days, and the formal test period lasted 28 days. After the growth performance test (day 36), 4 pigs were randomly selected from each group and transferred to pig metabolic cages for single cage feeding. The feeding and management were the same as the growth performance test. The test adaptation period was 3 days, and the pig feces were collected for 4 consecutive days, weighed and recorded, and stored in a -20℃ refrigerator.

[0031] 1.5 Growth performance

[0032] Fasting body weight was recorded in the morning at the beginning and end of the experiment (day 8 and day 35), and feed intake was recorded daily for calculation of average daily feed intake (ADFI), average daily gain (ADG), and feed to gain ratio (G:F).

[0033] Average daily feed intake (ADFI) = feed intake / days;

[0034] Daily weight gain (ADG) = (final weight - initial weight) / number of days;

[0035] Feed conversion rate = total weight gain (kg) / total feed consumed (kg).

[0036] 1.6 Nitrogen balance

[0037] After the growth performance test is completed, the pigs will be transferred to metabolic cages. The feeding and management are consistent with the growth performance test period, and the remaining feed of each pig in each meal is recorded. The first 3 days are the adaptation period, and then the pigs' feces and urine are collected for 4 consecutive days. After weighing and recording, they are stored in a -20°C refrigerator. After the collection is completed, the test samples are taken out and thawed, mixed and sampled in proportion. The taken samples are placed in a 65°C oven and dried for 72 hours. After drying, they are placed in a grinder and crushed for analysis. All feces, urine and feed samples are measured for nitrogen content using a fully automatic Kjeldahl nitrogen analyzer, and the data obtained are used to calculate the following parameters. All feces, urine and feed samples are measured for nitrogen content using a fully automatic Kjeldahl nitrogen analyzer, and the data obtained are used to calculate the following parameters.

[0038] Nitrogen intake (NI) = feed nitrogen content (%) × average daily feed intake (kg);

[0039] Fecal nitrogen excretion (FN) = fecal nitrogen content (%) × daily average fecal weight (kg);

[0040] Urinary nitrogen excretion (UN) = urine nitrogen content (%) × daily average urine volume (L);

[0041] Total nitrogen output (TN) = FN + UN;

[0042] Nitrogen deposition (RN) = NI-TN;

[0043] Apparent nitrogen digestibility (AND) = (NI−FN) / NI × 100%;

[0044] Nitrogen utilization efficiency (NPU) = RN / NI × 100%;

[0045] Apparent biological value of nitrogen (ABV) = RN / (NI−FN)×100%.

[0046] 1.7 Serum biochemical index test

[0047] On the morning of the 43rd day, 5 mL vacuum blood collection tubes were used to collect blood from the anterior vena cava of fasting pigs once before slaughter. The collected blood was left standing at room temperature for 1 h, then transferred to a centrifuge at 4 °C and centrifuged at 3000 r / min for 10 min. The serum samples obtained by centrifugation were detected and analyzed for various components in an automatic biochemical analyzer (BK1200, Biocase), including total protein (TP), albumin (ALB), globulin (GLB), serum urine nitrogen (SUN), glucose (GLU), alanine transaminase (ALT), and aspartate aminotransferase (AST).

[0048] 1.8 Data processing and analysis

[0049] All data were analyzed using the software SPSS 26.0. The analysis method was the univariate analysis method in the linear model. Each pig was taken as a test unit, and the test results were expressed as the mean with standard error. The significance of differences between treatments was compared by one-way analysis of variance. F represents the difference in feeding frequency levels; S represents the difference in protein sources; F×S represents the interaction between feeding frequency levels and protein sources. P<0.05 indicates significant differences, and 0.05<P<0.1 indicates a trend in differences. The significance of differences between treatments was compared by one-way analysis of variance.

[0050] 2 Results and analysis

[0051] 2.1 Effects of increasing feeding frequency on the growth performance of growing pigs fed different protein source diets

[0052] As shown in Table 1, ADFI was significantly affected by feeding frequency (P<0.05), and the ADFI of the T group was higher than that of the S group (P<0.05). There were significant differences in ADG and G:F among the four treatment groups in feeding frequency and protein source level (P<0.05). The ADG and G:F of the S group were higher than those of the T group (P<0.05); the ADG and G:F of the MLM group were significantly lower than those of the SBM group (P<0.05), among which the ADG of the T.MLM group was the lowest (P<0.05); and there were significant differences between the T.SBM group, the S.SBM group and the S.MLM group (P<0.05). However, there were no significant differences between the T.SBM group, the S.SBM group and the S.MLM group (P>0.05). However, it shows that increasing the feeding frequency can increase the ADG of the MLM diet for growing pigs. The G:F ratio of the S.SBM group was 3.71% higher than that of the T.SBM group, while that of the S.MLM group was 5.34% higher than that of the T.MLM group, indicating that increasing the feeding frequency can maximize the G:F ratio of the MLM diet.

[0053] 2.2 Effects of feeding different protein sources with increasing feeding frequency on nitrogen balance in growing pigs

[0054] As shown in Table 2, there were extremely significant differences in the feeding frequency levels of UN, NPU, and ABV among the four treatment groups ( P <0.01), there were significant differences in protein source level ( P <0.05), and there were significant differences in feeding frequency and protein source level among the four treatment groups ( P <0.05). RN showed a trend of significant differences in feeding frequency and protein source level among the four treatment groups, while NI showed no significant differences among the four treatment groups ( P <0.05). In addition, the two treatment factors of feeding frequency and protein source had interactive effects on FN and TN ( P <0.05), there was a trend of interactive effect among ADN, NPU, and ABV (0.1< P <0.05), and there was no interaction effect on the other indicators ( P >0.05). The FN and TN of the S.SBM group were reduced by 11.3% and 9.15% respectively compared with the T.SBM group, while the FN and TN of the T.MLM group were reduced by 19.3% and 16.4% respectively compared with the S.MLM group. At the same time, in the three indicators of ADN, NPU and ABV, there were no significant differences among the T.SBM group, S.SBM group and S.MLM group, but they were all significantly higher than the T.MLM group. This shows that increasing the feeding frequency can maximize the nitrogen utilization efficiency of the MLM group diet. These show that increasing the feeding frequency is more effective in reducing nitrogen emissions and improving nitrogen utilization efficiency in diets with poor synchronization of amino acid release.

[0055] 2.3 Effects of feeding different protein source diets with increasing feeding frequency on serum biochemical parameters of growing pigs

[0056] In Table 3, there were significant differences in the feeding frequency levels of SUN among the four treatment groups ( P <0.01), there were significant differences in protein source level ( P <0.05, and there was a trend of interaction between the two treatment factors (0.05< P <0.1). And the SUN of T.MLM group was the highest. In addition, there was no significant difference in TP, ALB, A / G, GLU, ALT and AST among the four treatment groups ( P >0.05). SUN showed extremely significant differences in feeding frequency levels and significant differences in protein source levels. There was also a trend of interaction between the two levels. The SUN of the S.SBM group was reduced by 13.8% compared with the T.SBM group, while the SUN of the T.MLM group was reduced by 23.5% compared with the S.MLM group. This shows that increasing the feeding frequency can better reduce the body's serum urea nitrogen in the MLM group diet. The change in serum urea nitrogen content can quickly reflect the animal's absorption and utilization of amino acids. When the serum contains a high level of SUN, it means that the protein utilization efficiency in the diet is low. Increasing the feeding frequency can maximize the protein utilization efficiency of the MLM diet. Except for SUN, no significant differences were found in the other indicators, indicating that the animals in each group were in good condition and that the immune function of the experimental pigs was normal.

[0057] The following conclusions were drawn from the above experiments:

[0058] First, on the basis of two feeds, the feeding frequency was increased from 2 times to 6 times, which slightly reduced the feed intake of growing pigs (p<0.05); the daily weight gain and feed utilization rate were significantly improved (P<0.05). At the same time, there was no significant difference between the unconventional protein source feed group fed 6 times a day and the other two soybean meal groups. By increasing the feeding frequency, the growth performance of unconventional protein source feed was improved, and the gap in growth performance between the same soybean meal feed was alleviated.

[0059] 2. On the basis of the two feeds, the feeding frequency was increased from 2 times to 6 times, which reduced the serum urea content of growing pigs. At the same time, the urea content of the group fed with unconventional protein source feed twice a day was higher than that of the other three groups (P<0.05). There was no effect on albumin, globulin, albumin-globulin ratio, alanine aminotransferase and aspartate aminotransferase (P>0.05).

[0060] 3. On the basis of two feeds, increasing the feeding frequency from 2 times to 6 times can reduce the nitrogen in manure, urine and total nitrogen emissions of growing pigs (P<0.05); and improve the digestibility, utilization and biological value of nitrogen in feed for growing pigs (P<0.05). Among them, increasing the feeding frequency from 2 times to 6 times can reduce the total nitrogen emissions of the 6-time non-conventional protein source group by 2.0%-3.0% (P<0.05); the digestibility, utilization and biological value of nitrogen in feed for growing pigs are increased by 3.0%-7.0% (P<0.05). The utilization efficiency of nitrogen in non-conventional protein source feeds is improved, and the gap in nitrogen utilization between the same soybean meal feeds is alleviated.

[0061] 4. According to the current price of feed raw materials, compared with soybean meal feed, the cost per ton of feed of unconventional protein source feed is reduced by 2.0%-4.0% (P<0.05), which improves the feed return.

[0062] In summary, there is an interactive effect between feeding frequency and protein source level on nitrogen utilization in growing pigs. Increasing feeding frequency can maximize nitrogen utilization efficiency and growth performance of MLM diets.

[0063] Table 1 Effects of different feeding frequencies and protein sources on growth performance of growing pigs

[0064]

[0065] Note: ①2: diet group fed twice a day; 6: diet group fed 6 times a day; SBM: corn-soybean meal diet group; MLM: corn-unconventional protein source growing pig diet group; T.SBM: corn-soybean meal diet group fed twice a day; S.SBM: corn-soybean meal diet group fed 6 times a day; T.MLM: corn-unconventional protein source growing pig diet group fed twice a day; S.MLM: corn-unconventional protein source growing pig diet group fed 6 times a day; SEM: standard error; F: feeding frequency; S: protein source; F*S: interaction between feeding frequency and protein source.

[0066] ②: Within the same experimental factors a, b, and c (feeding frequency, protein source, and treatment group), different letters in the shoulders of each index indicate significant differences among the groups (P<0.05), and the same letters or no letters indicate no significant differences (P>0.05).

[0067] Table 2 Effects of different feeding frequencies and protein sources on nitrogen balance in growing pigs

[0068]

[0069] Note: ①2: diet group fed twice a day; 6: diet group fed 6 times a day; SBM: corn-soybean meal diet group; MLM: corn-unconventional protein source growing pig diet group; T.SBM: corn-soybean meal diet group fed twice a day; S.SBM: corn-soybean meal diet group fed 6 times a day; T.MLM: corn-unconventional protein source growing pig diet group fed twice a day; S.MLM: corn-unconventional protein source growing pig diet group fed 6 times a day; SEM: standard error; F: feeding frequency; S: protein source; F*S: interaction between feeding frequency and protein source.

[0070] ②: Within the same experimental factors a, b, and c (feeding frequency, protein source, and treatment group), different letters in the shoulders of each index indicate significant differences among the groups (P<0.05), and the same letters or no letters indicate no significant differences (P>0.05).

[0071] Table 3 Effects of different feeding frequencies and protein sources on serum biochemical parameters of growing pigs

[0072]

[0073] Note: ①2: diet group fed twice a day; 6: diet group fed 6 times a day; SBM: corn-soybean meal diet group; MLM: corn-unconventional protein source growing pig diet group; T.SBM: corn-soybean meal diet group fed twice a day; S.SBM: corn-soybean meal diet group fed 6 times a day; T.MLM: corn-unconventional protein source growing pig diet group fed twice a day; S.MLM: corn-unconventional protein source growing pig diet group fed 6 times a day; SEM: standard error; F: feeding frequency; S: protein source; F*S: interaction between feeding frequency and protein source.

[0074] ②: Within the same experimental factors a, b, and c (feeding frequency, protein source, and treatment group), different letters in the shoulders of each index indicate significant differences among the groups (P<0.05), and the same letters or no letters indicate no significant differences (P>0.05).

Claims

1. A method for feeding a corn-unconventional protein source diet to growing pigs, characterized in that The feeding method of the corn-unconventional protein source growing pig diet is feeding 6 times a day, and the corn-unconventional protein source growing pig diet is as follows: 55.0-65.0 parts of corn, 1.0-5.0 parts of wheat bran, 15.0-20.0 parts of soybean meal, 1.5-3.0 parts of rapeseed meal, 1.5-3.0 parts of cottonseed meal, 6.0-10.0 parts of dry corn alcohol grains, 1.0-3.0 parts of sucrose, 1.0-3.0 parts of soybean oil, 0.1-0.5 parts of lysine hydrochloride, 0.01-0.05 parts of methionine, 0.05-0.1 parts of cysteine, 0.04-0.1 parts of threonine, 1.0-1.5 parts of stone powder, 1.0-1.5 parts of calcium hydrogen phosphate, 0.5-1.0 parts of sodium chloride and 1.0 parts of premix; The premix consists of vitamin A, vitamin D, vitamin E, vitamin K, vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, folic acid, calcium pantothenate, antioxidant, choline chloride, CoSO4, CuSO4·5H2O, FeSO4·H2O, MnO, ZnSO4, KI and Na2SeO3, and contains 28,500 IU of vitamin A, 36,000 IU of vitamin D, 67.5 IU of vitamin E, 37.5 mg of vitamin K, 17.5 mg of vitamin B1, 215 mg of vitamin B2, 69 mg of vitamin B6, 0.075 mg of vitamin B12, 70 mg of niacin, 3 mg of folic acid, 0.375 mg of calcium pantothenate, 0.15 mg of antioxidant, 105 mg of choline chloride, 1 mg of CoSO4, 1 mg of CuSO4·5H2O, 155mg, FeSO4·H2O 145mg, MnO 75mg, ZnSO4 125mg, KI 0.3mg, Na2SeO3 0.3mg.

2. The feeding method of the corn-unconventional protein source growing pig diet according to claim 1, characterized in that The corn-unconventional protein source growing pig diet is as follows: it consists of 60.12 parts of corn, 2.00 parts of wheat bran, 16.86 parts of soybean meal, 2.57 parts of rapeseed meal, 2.50 parts of cottonseed meal, 7.50 parts of dry corn distillers grains, 1.35 parts of sucrose, 2.79 parts of soybean oil, 0.32 parts of lysine hydrochloride, 0.03 parts of methionine, 0.08 parts of cysteine, 0.04 parts of threonine, 1.04 parts of stone powder, 1.00 parts of calcium hydrogen phosphate, 0.8 parts of sodium chloride and 1.0 parts of premix in parts by weight.

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