A stem cell preparation apparatus and method

Abstract

The present application relates to stem cell cleaning technical field, with relate to a kind of stem cell preparation equipment, including the box with import and export being opened;It further includes the cleaning mechanism being arranged in the box;Cleaning mechanism includes inclined cylinder, the storage box with feed hole being opened in top, the cleaning assembly being arranged in inclined cylinder, the feed assembly for intermittently introducing the tissue block in storage box into inclined cylinder, the water outlet assembly for draining to inclined cylinder;Inclined cylinder is fixedly connected with the box, and the end of inclined cylinder close to the bottom of the box is provided with through-hole;Storage box is fixedly connected with the top of the box, and feed hole is communicated with import;Cleaning assembly includes shaft, filter screen, a plurality of first bristle blocks, filter hole being opened in the bottom of inclined cylinder, the driving portion for driving shaft rotation;Filter screen is fixedly connected with filter hole;First bristle block is fixedly connected with shaft;To solve the problem that existing preparation device cannot clean the residue remaining on filter screen during filtering tissue block.

Description

Technical Field

[0001] This invention relates to the field of stem cell cleaning technology, specifically to a stem cell preparation device and method. Background Technology

[0002] In stem cell research, researchers need to collect a large number of primary cells to meet experimental requirements, which requires collecting a large number of tissue blocks. After collection, the tissue blocks need to be cleaned. Currently, the cleaning of tissue blocks is generally done manually. Manual cleaning is not only time-consuming but also exhausting for the researchers, affecting the progress of the experiment.

[0003] To address the aforementioned issues, Chinese Patent Publication No. CN220485695U discloses a stem cell preparation device, comprising a washing cylinder. Electric telescopic rods are fixedly connected to both the left and right side walls of the washing cylinder. A cover plate is fixedly connected to the output ends of the two electric telescopic rods, located at the top of the washing cylinder. A mounting frame is fixedly connected to the bottom of the washing cylinder's cavity, and a filter assembly is slidably connected inside the mounting frame. A mounting plate is rotatably connected to the bottom of the washing cylinder's cavity via a rotating shaft. This device utilizes the rotation of the support cylinder and the mutual impact generated by the washing liquid, causing the tissue blocks inside the support cylinder to be automatically tumbled by the washing liquid. This eliminates the need for manual cleaning, reducing the labor intensity of workers and improving work efficiency.

[0004] The above-mentioned device has the following problems in actual use: 1. The device requires staff to regularly disassemble and remove the filter screen to clean the tissue debris adhering to it, so as to ensure that the filter screen does not become clogged. However, after cleaning the filter screen only once, some tissue debris will still remain on the filter screen, which will affect the reuse of the filter screen and reduce the filtration efficiency. If the staff cleans the filter screen multiple times, it will increase the workload of the staff, make the work tedious, and may even lead to incomplete cleaning of the filter screen, thus affecting the reuse of the filter screen; 2. When disassembling the filter screen, the filter screen may shake. Therefore, during the disassembly process, some tissue debris will adhere to the staff's body. This means that after cleaning the filter screen, the staff will have to wash off the tissue debris adhering to their body, which will bring unnecessary trouble to the staff's work. Summary of the Invention

[0005] This invention provides a stem cell preparation device to solve the problem that existing preparation devices cannot clean the residue remaining on the filter screen during the filtration of tissue blocks.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a stem cell preparation device, comprising a box with an inlet and an outlet; further comprising a cleaning mechanism disposed within the box; the cleaning mechanism comprising an inclined cylinder, a storage box with a feed hole at the top, a cleaning component disposed within the inclined cylinder, a feed component for intermittently introducing tissue blocks from the storage box into the inclined cylinder, and a water outlet component for draining water into the inclined cylinder; the inclined cylinder is fixedly connected to the box, and a through hole is opened at one end of the inclined cylinder near the bottom of the box, the through hole communicating with the outlet; the storage box is fixedly connected to the top of the box, and the feed hole communicating with the inlet; the cleaning component comprises a rotating shaft, a filter screen, a plurality of first brush bristles, a filter hole at the bottom of the inclined cylinder, and a drive unit for driving the rotating shaft to rotate; the filter screen is fixedly connected to the filter hole; the plurality of first brush bristles are equidistantly arranged along the axial direction of the rotating shaft, the first brush bristles are fixedly connected to the rotating shaft, the first brush bristles are located on the water outlet path of the water outlet component, and the filter screen is located on the movement trajectory of the first brush bristles.

[0007] The principle and advantages of this scheme are:

[0008] 1. The tissue block is intermittently introduced into the inclined cylinder by the feeding component, which causes the tissue block to move towards the outlet along the inclined direction of the cylinder. During the movement of the tissue block, the water discharge component continuously drains water into the inclined cylinder, thereby cleaning the tissue block and ensuring that there is no residue on the tissue block when it is discharged from the outlet, which prepares it for further operations on the tissue block.

[0009] During the movement of the tissue blocks, the tissue blocks are intermittently introduced into the inclined cylinder through the feeding component. On the one hand, this ensures that the movement of the tissue blocks in the inclined cylinder is orderly, thus avoiding the possibility of clogging the inclined cylinder due to excessive movement of tissue blocks at the same time. On the other hand, since only a small batch of tissue blocks are moving in the inclined cylinder, it can ensure that the tissue blocks are cleaned more completely and thoroughly under the action of water, thereby improving the quality of the tissue blocks when they are discharged from the outlet.

[0010] 2. During the movement of the tissue block inside the inclined cylinder, the drive unit drives the rotating shaft to rotate; during the rotation of the rotating shaft, the first brush block rotates synchronously; during the rotation of the first brush block, the first brush block can move the residue with strong adhesion on the tissue block, so that the residue with strong adhesion no longer adheres to the tissue block, further improving the quality of the tissue block discharged from the outlet.

[0011] Furthermore, since the filter screen is located on the movement trajectory of the first brush block, the first brush block can move the residue that is blocked on the filter screen, so that the residue will no longer block the filter screen, thereby enhancing the filtration effect of the filter screen and improving the filtration efficiency of the filter screen.

[0012] 3. The filter screen is designed to remove the mixture of water and residue, allowing clean tissue blocks to be discharged through the outlet, thus facilitating collection and storage by staff.

[0013] In summary, through the combined action of the feeding component, the water outlet component, the first brush block, and the filter screen, the operation of feeding, water outlet, agitation, filtration, and discharge is integrated, which shortens the workflow, saves workers unnecessary trouble, and improves work efficiency.

[0014] Furthermore, several blocking blocks are equidistantly arranged along the axial direction of the rotating shaft. The blocking blocks are located between two adjacent first bristle blocks, and the blocking blocks are fixed to the rotating shaft. The two adjacent blocking blocks are staggered.

[0015] During the movement of the tissue block inside the inclined cylinder, the staggered movement of several blocking blocks can prolong the movement time of the tissue block inside the inclined cylinder, so that water and the first bristle block can come into more full and complete contact with the tissue block, that is, all the residue on the tissue block can be removed.

[0016] Furthermore, it also includes a filtration section; the filtration section includes an inclined block, a moving block, a filter cloth, several cleaning blocks, filter holes opened on the bottom of the inclined block, and a motion unit for driving the moving block to reciprocate along the length of the inclined block; the inclined block is located directly below the inclined cylinder, the inclined block is fixedly connected to the inclined cylinder, and the end of the inclined block near the bottom of the box faces the outlet; the filter cloth is fixedly connected to the filter holes; several cleaning blocks are all fixedly connected to the moving block, and the end of the cleaning block away from the moving block is in contact with the filter cloth.

[0017] The moving unit drives the moving block to reciprocate along the length of the inclined block. During the movement of the moving block, the cleaning block moves synchronously. Therefore, the residue remaining on the filter cloth surface will move along the length of the inclined block towards the position near the outlet under the action of the cleaning block, so that the residue can be discharged through the outlet, completing the separation of water and residue and ensuring that the environment is not polluted.

[0018] The filter cloth is designed to separate water and residue. This makes it easier for staff to clean the residue and ensures that the water can be reused after discharge, thus improving water utilization. Furthermore, the filtered water will not pollute the environment.

[0019] In summary, by combining the cleaning block and the filter cloth, the filtration capacity of the filter cloth can be improved, achieving efficient filtration of the mixed liquid. On the other hand, the residue remaining on the filter cloth can be recovered, ensuring that the residue does not accumulate on the filter cloth and improving the residue recovery rate.

[0020] Furthermore, it also includes a linkage part disposed on the moving block; the linkage part includes a vertical block, an arc-shaped block, a short shaft, a first cam, a first spring, several second brush bristle blocks, a side hole opened on the vertical block, and a linkage unit for driving the short shaft to rotate; the vertical block is fixedly connected to the moving block; the arc-shaped block is vertically slidably connected to the side hole, and the arc-shaped block can move vertically within the side hole; the short shaft is rotatably connected to the vertical block; the first cam is fixedly connected to the short shaft, and the first cam abuts against the arc-shaped block; the two ends of the first spring are respectively connected to the side hole and the arc-shaped block; several second brush bristle blocks are all fixedly connected to the arc-shaped block, and the filter screen is located on the movement trajectory of the second brush bristle blocks.

[0021] During the reciprocating motion of the moving block along the length of the inclined block, the short shaft moves synchronously. During the movement of the short shaft, the linkage unit drives the short shaft to rotate. Therefore, while the short shaft reciprocates along the length of the inclined block, it can also rotate. During the rotation of the short shaft, the first cam rotates synchronously. During the rotation of the first cam, when the protrusion of the first cam abuts against the arc-shaped block, the arc-shaped block moves vertically upward, compressing the first spring. When the protrusion of the first cam no longer abuts against the arc-shaped block, the arc-shaped block returns to its original position under the action of the first spring, and the arc-shaped block moves vertically downward. Therefore, while the arc-shaped block reciprocates along the length of the inclined block, it can also reciprocate vertically.

[0022] During the movement of the arc-shaped block, the second brush block moves synchronously. Through the reciprocating motion of the second brush block along the length of the inclined block and its vertical reciprocating motion, on the one hand, when the second brush block moves vertically, it can extend into the holes of the filter screen, causing the residue clogging the holes to move from bottom to top and eventually fall onto the surface of the filter screen, no longer clogging the holes; on the other hand, when the second brush block moves reciprocating along the length of the inclined block, it can ensure that all holes at any position along the length of the filter screen are cleaned, further ensuring that no residue clogs the holes of the filter screen, thereby improving the filtration capacity of the filter screen.

[0023] In summary, by using the first brush block to clean the residue on the surface of the filter screen and the second brush block to clean the residue in the filter screen holes, the filter screen is fully protected from clogging due to the action of the bristles, further enhancing the filtration effect and ensuring that the filter screen is always in a highly efficient filtration state.

[0024] Furthermore, the water outlet assembly includes a water tank, a piston cylinder, a piston block, a second cam, and a second spring; the water tank is fixedly connected to the housing; the piston cylinder is fixedly connected to the housing; the piston block is slidably connected to the piston cylinder; the second cam is fixedly connected to the rotating shaft and abuts against the piston block; the two ends of the second spring are respectively connected to the piston cylinder and the piston block; an inlet pipe and a drain pipe are connected to the piston cylinder; the inlet pipe is connected to the water tank; several drain holes are opened on the drain pipe; several side holes are opened on the inclined cylinder, the side holes are connected to the drain holes, and the side holes are located on the movement trajectory of the first brush block.

[0025] During the rotation of the shaft, the second cam rotates synchronously; during the rotation of the second cam, when the protrusion of the second cam abuts against the piston block, the piston block moves vertically upward, and the second spring is compressed; when the protrusion of the second cam no longer abuts against the piston block, the piston block returns to its original position under the action of the second spring, and the piston block moves vertically downward; therefore, the piston block can perform vertical reciprocating motion.

[0026] During the vertical reciprocating motion of the piston block, the piston block can continuously pump water and drain water. During the pumping of water, the water enters the piston cylinder through the water tank and is temporarily stored. During the draining of water, the water stored in the piston cylinder flows through the drain pipe and side hole into the inclined cylinder to clean the tissue block, ensuring that the residue on the tissue block is cleaned by the water and improving the quality of the tissue block when it is discharged.

[0027] Furthermore, the feeding assembly includes a discharge hole on the storage box, a baffle for sealing the discharge hole, and a top hole on the top of the inclined cylinder; the baffle is attached to the outer wall of the storage box and is fixedly connected to the piston block; the discharge hole is located above the top hole and can communicate with the top hole.

[0028] During the reciprocating motion of the piston block, the baffle moves synchronously. When the baffle moves vertically upward, it no longer seals the discharge hole, allowing the discharge hole to communicate with the top hole. The tissue block located in the storage box can fall into the inclined cylinder through the top hole, completing the feeding. When the baffle moves vertically downward, it seals the discharge hole again, preventing the tissue block located in the storage box from being discharged, thus blocking it. Therefore, the intermittent communication between the baffle and the discharge hole enables the intermittent feeding of the tissue block.

[0029] Furthermore, it also includes a recycling section; the recycling section includes a recycling tank and a recycling pipe; the recycling tank is fixedly connected to the housing; one end of the recycling pipe is connected to the piston cylinder, and the other end of the recycling pipe extends into the recycling tank.

[0030] The recycling tank is designed to collect the water filtered out by the filter cloth, enabling water reuse. The reciprocating motion of the piston block allows the water to flow back into the piston cylinder through the recycling pipe, thus allowing the filtered water to be applied to the tissue block again under the action of the piston block, improving water utilization.

[0031] Furthermore, the motion unit includes a screw, a belt, and a side groove on the side wall of the inclined block; the screw is rotatably connected to the housing; both ends of the belt are sleeved between the rotating shaft and the screw; the moving block is threadedly connected to the screw and slidably connected to the side groove.

[0032] During the rotation of the shaft, the screw rotates synchronously under the drive of the belt; during the forward rotation of the screw, the moving block moves towards the bottom of the housing along the length of the inclined block; during the reverse rotation of the screw, the moving block moves away from the bottom of the housing along the length of the inclined block; therefore, the moving block can reciprocate along the length of the inclined block.

[0033] Furthermore, the linkage unit includes a gear and a rack; the gear is fixedly connected to the short shaft; the rack is fixedly connected to the inclined block, and the gear and rack mesh.

[0034] During the reciprocating motion of the short shaft along the length of the inclined block, the gear moves synchronously; during the gear's motion, the gear rotates by meshing with the rack; during the gear's rotation, the short shaft rotates synchronously. Therefore, while the short shaft reciprocates along the length of the inclined block, it can also rotate.

[0035] The present invention aims to provide a cleaning method to solve the problem that existing preparation devices cannot clean the residue remaining on the filter screen during the filtration of tissue blocks.

[0036] To achieve the above objectives, the present invention adopts the following technical solution: a cleaning method, comprising the following steps:

[0037] Step (1): Staff members place the tissue block into the storage box along the inlet for storage;

[0038] Step (2): Through the reciprocating motion of the piston block, water enters the inclined cylinder along the drain pipe on the one hand, and tissue blocks intermittently enter the inclined cylinder along the top hole on the other hand.

[0039] Step (3): Through the combined action of the first brush block and the blocking block, the time the tissue block stays in the inclined cylinder is extended on the one hand, and the residue with strong adhesion on the surface of the tissue block is cleaned on the other hand.

[0040] Step (4): By moving the block back and forth, the cleaning block cleans the residue on the surface of the filter cloth, and the second brush block cleans the residue inside the filter screen.

[0041] Step (5): Through the combined action of the recycling tank and the recycling pipe, the water is recycled.

[0042] The principles and advantages of this scheme are:

[0043] 1. By intermittently connecting the baffle and the discharge hole and injecting water into the inclined cylinder through the drain pipe, on the one hand, the movement of tissue blocks in the inclined cylinder can be ensured in an orderly manner, thus avoiding the situation where excessive tissue blocks move at the same time and may cause blockage of the inclined cylinder. On the other hand, since only a small batch of tissue blocks are moving in the inclined cylinder, it can be ensured that the tissue blocks are cleaned more completely and thoroughly under the action of water, thereby improving the quality of the tissue blocks when they are discharged from the outlet.

[0044] 2. Through the rotation of several first brush bristle blocks and the staggered movement of several blocking blocks, on the one hand, the blocking blocks can prolong the movement time of the tissue block in the inclined cylinder, so that the water and the first brush bristle blocks can come into more sufficient and complete contact with the tissue block, that is, the residue on the tissue block can be completely removed; on the other hand, the first brush bristle blocks can move the residue with strong adhesion on the tissue block, so that the residue with strong adhesion no longer adheres to the tissue block, further improving the quality of the tissue block discharged from the outlet; and, since the filter screen is located on the movement trajectory of the first brush bristle blocks, the first brush bristle blocks can move the residue that is blocked on the filter screen, so that the residue no longer blocks the filter screen, thereby enhancing the filtration effect of the filter screen.

[0045] 3. During the movement of the moving block, on the one hand, it can drive the cleaning block to clean the residue remaining on the surface of the filter cloth, thereby improving the filtration capacity of the filter cloth, that is, efficiently separating water and residue. On the other hand, it can drive the second brush block to clean the holes at any position along the length of the filter screen, ensuring that residue will not clog the holes, further enhancing the filtration effect of the filter screen.

[0046] 4. The purpose of the recycling tank is to collect the water filtered out by the filter cloth and realize the secondary use of water. Then, through the reciprocating motion of the piston block, the water flows back into the piston cylinder along the recycling pipe, so that the filtered water can be applied to the tissue block again under the action of the piston block, thereby improving the water utilization rate. Attached Figure Description

[0047] Figure 1 This is a schematic diagram of the structure of the housing in an embodiment of a stem cell preparation device of the present invention.

[0048] Figure 2 for Figure 1 A sectional view of the middle box from the front view direction.

[0049] Figure 3 for Figure 2 A schematic diagram of the cleaning mechanism inside the middle chamber.

[0050] Figure 4 for Figure 3 Enlarged view of point A in the middle.

[0051] Figure 5 for Figure 3 A schematic diagram of a partial structure inside the inclined cylinder viewed from below.

[0052] Figure 6 for Figure 5 Enlarged view of point B in the middle. Detailed Implementation

[0053] The following detailed description illustrates the specific implementation methods:

[0054] The reference numerals in the accompanying drawings include: 1. Box body; 2. Inclined cylinder; 3. Storage box; 4. First hollow cylinder; 5. Rotating shaft; 6. Filter screen; 7. First brush block; 8. Servo motor; 9. Blocking block; 10. Inclined block; 11. Moving block; 12. Filter cloth; 13. Cleaning block; 14. Second hollow cylinder; 15. Vertical block; 16. Arc-shaped block; 17. Short shaft; 18. First cam; 19. First spring; 20. Second brush block; 21. Water tank; 22. Piston cylinder; 23. Piston block; 24. Second cam; 25. Second spring; 26. Water inlet pipe; 27. Drain pipe; 28. Side hole; 29. ​​Baffle; 30. Recycling tank; 31. Recycling pipe; 32. Screw; 33. Belt; 34. Gear; 35. Rack; 36. Inlet; 37. Outlet; 38. Top hole; 39. Side hole; 40. Horizontal rod.

[0055] The basic implementation examples are as follows: Figure 1 , 2 As shown in points 3, 4, 5, and 6:

[0056] An embodiment of the present invention provides a stem cell preparation device, including a box 1 with an inlet 36 at the top and an outlet 37 at the bottom; it also includes a cleaning mechanism disposed within the box 1; the cleaning mechanism includes an inclined cylinder 2, a storage box 3 with a feed hole at the top, a cleaning component disposed within the inclined cylinder 2, a feed component for intermittently introducing tissue blocks from the storage box 3 into the inclined cylinder 2, and a drain component for draining water into the inclined cylinder 2; the inclined cylinder 2 is fixedly connected to the inner wall of the box 1, and a through hole is opened at one end of the inclined cylinder 2 near the bottom of the inner wall of the box 1; a first hollow cylinder 4 is disposed between the through hole and the outlet 37; the first hollow cylinder 4 is fixedly connected to the bottom of the inner wall of the box 1, and both ends of the first hollow cylinder 4 are connected to the through hole and the outlet 37. The inlet 37 is connected to the outlet 3; the storage box 3 is fixedly connected to the top of the inner wall of the box 1, and the feed hole is connected to the inlet 36; the cleaning assembly includes a rotating shaft 5, a filter screen 6, several first bristle blocks 7, a filter hole opened at the bottom of the inclined cylinder 2, and a drive unit for driving the rotating shaft 5 to rotate; the filter screen 6 is fixedly connected to the filter hole, and the filter screen 6 can seal the filter hole; several first bristle blocks 7 are equidistantly arranged along the axial direction of the rotating shaft 5, and the first bristle block 7 includes several bristle layers, the bristle layers are fixedly connected to the rotating shaft 5, the bristle layers are located on the water outlet path of the water outlet assembly, and the filter screen 6 is located on the movement trajectory of the bristle layers; the drive unit is a servo motor 8, the servo motor 8 is fixedly connected to the inner wall of the box 1, and the output shaft of the servo motor 8 is fixedly connected to the rotating shaft 5.

[0057] Several blocking blocks 9 are equidistantly arranged along the axial direction of the rotating shaft 5. The blocking blocks 9 are located between two adjacent first brush blocks 7. The blocking blocks 9 are fixed to the rotating shaft 5, and the two adjacent blocking blocks 9 are staggered. The blocking blocks 9 are fan-shaped, and the radius of the blocking blocks 9 is equal to the radius of the inner wall of the inclined cylinder 2.

[0058] It also includes a filtration section; the filtration section includes an inclined block 10, a moving block 11, a filter cloth 12, several cleaning blocks 13, filter holes opened on the bottom of the inclined block 10, and a motion unit for driving the moving block 11 to reciprocate along the length direction of the inclined block 10; the inclined block 10 is located directly below the inclined cylinder 2, the inclined block 10 is fixed to the outer wall of the inclined cylinder 2, the length direction of the inclined block 10 is parallel to the axial direction of the inclined cylinder 2, a second hollow cylinder 14 is provided at one end of the inclined block 10 near the bottom of the inner wall of the housing 1, the second hollow cylinder 14 is fixed to the bottom of the inner wall of the housing 1; the end of the inclined block 10 near the bottom of the inner wall of the housing 1 faces the second hollow cylinder 14, the second hollow cylinder 14 communicates with the outlet 37; the filter cloth 12 is fixed to the filter holes, and the filter cloth 12 can seal the filter holes; several cleaning blocks 13 are equidistantly arranged along the length direction of the moving block 11, the cleaning blocks 13 are fixed to the bottom of the moving block 11, and the end of the cleaning block 13 away from the moving block 11 is in contact with the filter cloth 12.

[0059] It also includes a linkage part disposed on the movable block 11; the linkage part includes a vertical block 15, an arc-shaped block 16, a short shaft 17, a first cam 18, a first spring 19, a plurality of second bristle blocks 20, a side hole opened on the vertical block 15, and a linkage unit for driving the short shaft 17 to rotate; the vertical block 15 is fixedly connected to the top of the movable block 11; a horizontal rod 40 is fixedly connected to the arc-shaped block 16, the horizontal rod 40 is vertically slidably connected to the side hole, and the horizontal rod 40 can move vertically in the side hole; the short shaft 17 is rotatably connected to the vertical block 15; the first cam 18 is fixedly connected to the short shaft 17, and the first cam 18 abuts against the horizontal rod 40; the two ends of the first spring 19 are respectively connected to the side hole and the horizontal rod 40; the plurality of second bristle blocks 20 are all fixedly connected to the top of the arc-shaped block 16, and the filter screen 6 is located on the movement trajectory of the second bristle blocks 20.

[0060] The water outlet assembly includes a water tank 21, a piston cylinder 22, a piston block 23, a second cam 24, and a second spring 25. The water tank 21 is fixedly connected to the top of the outer wall of the tank body 1; the piston cylinder 22 is fixedly connected to the top of the inner wall of the tank body 1; the piston block 23 is slidably connected to the inner wall of the piston cylinder 22; the second cam 24 is fixedly connected to the rotating shaft 5 and abuts against the piston block 23; the second spring 25 is sleeved on the piston block 23, and its two ends are respectively connected to the piston cylinder 22 and the piston block 23; the piston cylinder 22 is connected to an inlet valve. Water pipe 26 and drain pipe 27; water inlet pipe 26 is connected to water tank 21, and water inlet pipe 26 is provided with a first one-way valve for liquid to flow unidirectionally from water tank 21 to piston cylinder 22; drain pipe 27 is attached to the outer wall of inclined cylinder 2, and several drain holes are equidistantly opened on drain pipe 27; several side holes 28 are equidistantly opened on inclined cylinder 2, the side holes 28 are connected to the drain holes, and the side holes 28 are located on the movement trajectory of the brush layer; drain pipe 27 is provided with a second one-way valve for liquid to flow unidirectionally from piston cylinder 22 to inclined cylinder 2.

[0061] The feeding assembly includes a discharge hole on the side wall of the storage box 3, a baffle 29 for sealing the discharge hole, and a top hole 38 on the top of the inclined cylinder 2; the baffle 29 is located between the discharge hole and the top hole 38, the baffle 29 is attached to the outer wall of the storage box 3, and the baffle 29 is fixedly connected to the piston block 23; the discharge hole is located above the top hole and can communicate with the top hole 38.

[0062] It also includes a recovery section; the recovery section includes a recovery tank 30 and a recovery pipe 31; the recovery tank 30 is fixedly connected to the inner wall of the housing 1; one end of the recovery pipe 31 is connected to the piston cylinder 22, and the other end of the recovery pipe 31 extends into the recovery tank 30; the recovery pipe 31 is provided with a third one-way valve for liquid to flow unidirectionally from the recovery tank 30 to the piston cylinder 22.

[0063] The motion unit includes a screw 32, a belt 33, and a side groove on the side wall of the inclined block 10; the screw 32 is located between the inclined cylinder 2 and the inclined block 10, and the screw 32 is rotatably connected to the inner wall of the housing 1; the two ends of the belt 33 are sleeved between the rotating shaft 5 and the screw 32; the moving block 11 is threadedly connected to the screw 32, and the moving block 11 is slidably connected to the side groove.

[0064] The linkage unit includes a gear 34 and a rack 35; the gear 34 is fixedly connected to the short shaft 17; the rack 35 is fixedly connected to the inner wall of the inclined block 10, and the gear 34 and the rack 35 mesh.

[0065] The specific implementation process is as follows:

[0066] Staff members place the tissue blocks required for the experiment into the storage box 3 along the inlet 36 for temporary storage, to facilitate subsequent cleaning. After the tissue blocks are placed, the servo motor 8 is started, and the output shaft of the servo motor 8 drives the rotating shaft 5 to rotate. During the rotation of the rotating shaft 5, the second cam 24 rotates synchronously. During the rotation of the second cam 24, when the protrusion of the second cam 24 abuts against the piston block 23, the piston block 23 moves vertically upward, and the second spring 25 is compressed. When the protrusion of the second cam 24 no longer abuts against the piston block 23, the piston block 23 returns to its original position under the action of the second spring 25, and the piston block 23 moves vertically downward. Therefore, the piston block 23 can perform vertical reciprocating motion.

[0067] During the reciprocating motion of piston block 23, baffle 29 moves synchronously. During the vertical upward movement of baffle 29, baffle 29 no longer seals the discharge hole, allowing the discharge hole to communicate with the top hole 38. The tissue block located in storage box 3 can fall into inclined cylinder 2 through top hole 38, completing the feeding. During the vertical downward movement of baffle 29, baffle 29 seals the discharge hole again, preventing the tissue block located in storage box 3 from being discharged, thus completing the blocking. Therefore, through the intermittent communication between baffle 29 and discharge hole, intermittent feeding of tissue block is achieved.

[0068] The vertical reciprocating motion of the baffle 29 allows the tissue block to intermittently enter the inclined cylinder 2, enabling the tissue block in the inclined cylinder 2 to move towards the position of the first hollow cylinder 4 along the inclined direction of the inclined cylinder 2. During the movement of the tissue block, the reciprocating motion of the piston block 23 allows the piston block 23 to continuously pump water and drain water. During the pumping of water by the piston block 23, water enters the piston cylinder 22 through the water tank 21 and is temporarily stored. During the draining of water by the piston block 23, the water stored in the piston cylinder 22 flows into the inclined cylinder 2 through the drain pipe 27 and the side hole 28, completing the cleaning of the tissue block. This ensures that the residue on the tissue block is cleaned by the water, improving the quality of the tissue block when it is discharged and preparing for further operations on the tissue block.

[0069] Through the aforementioned movement, the tissue blocks are intermittently introduced into the inclined cylinder 2 using the baffle 29. On the one hand, this ensures that the movement of the tissue blocks within the inclined cylinder 2 is orderly, thus avoiding the possibility of clogging the inclined cylinder 2 due to excessive movement of tissue blocks at the same time. On the other hand, since only a small batch of tissue blocks are moving within the inclined cylinder 2, it ensures that the tissue blocks are cleaned more thoroughly and fully under the action of water, further improving the cleanliness of the tissue blocks when they are discharged from the outlet 37.

[0070] During the movement of the tissue block inside the inclined cylinder 2, the rotation of the rotating shaft 5 causes the first brush block 7 to rotate synchronously. During the rotation of the first brush block 7, the first brush block 7 can move the residue with strong adhesion on the tissue block, so that the residue with strong adhesion no longer adheres to the tissue block, further ensuring the quality of the tissue block when it is discharged from the outlet 37.

[0071] Furthermore, since the filter screen 6 is located on the movement trajectory of the first brush block 7, the first brush block 7 can move the residue that is blocked on the filter screen 6, so that the residue will no longer block the filter screen 6, thereby enhancing the filtration effect of the filter screen 6 and improving the filtration efficiency of the filter screen 6.

[0072] The filter screen 6 is designed to filter out the mixture of water and residue, allowing clean tissue blocks to be discharged through outlet 37, thus facilitating collection and storage by staff.

[0073] During the movement of the tissue block within the inclined cylinder 2, the rotating shaft 5 also drives several blocking blocks 9 to rotate. Through the staggered movement of these blocking blocks 9, the movement time of the tissue block within the inclined cylinder 2 can be extended, allowing water and the first brush bristle block 7 to come into more thorough and complete contact with the tissue block, meaning that all residue on the tissue block can be removed.

[0074] In summary, through the combined action of baffle 29, drain pipe 27, first brush block 7, blocking block 9, and filter screen 6, the operation of feeding, water discharge, stirring, filtering, and discharging is integrated, which shortens the workflow, saves workers unnecessary trouble, and improves work efficiency.

[0075] During the rotation of the rotating shaft 5, the rotating shaft 5 drives the screw 32 to rotate via the belt 33. During the rotation of the screw 32, when the screw 32 rotates forward, the moving block 11 moves towards the bottom of the box 1 along the length direction of the inclined block 10. When the screw 32 rotates in reverse, the moving block 11 moves away from the bottom of the box 1 along the length direction of the inclined block 10. Therefore, the moving block 11 can reciprocate along the length direction of the inclined block 10.

[0076] During the movement of the moving block 11, the cleaning block 13 moves synchronously; therefore, the residue remaining on the surface of the filter cloth 12 will move along the length of the inclined block 10 towards the location of the second hollow cylinder 14 under the action of the cleaning block 13, so that the residue can be discharged through the outlet 37 along the second hollow cylinder 14, completing the separation of water and residue, and ensuring that the environment is not polluted.

[0077] The filter cloth 12 is designed to separate water and residue. This makes it easier for staff to clean the residue and ensures that the water can be reused after discharge, thus improving water utilization. Furthermore, the filtered water will not pollute the environment.

[0078] In summary, through the combined action of the cleaning block 13 and the filter cloth 12, the filtration capacity of the filter cloth 12 can be improved, achieving efficient filtration of the mixed liquid. On the other hand, the residues remaining on the filter cloth 12 can be recovered, ensuring that the residues do not accumulate on the filter cloth 12 and improving the residue recovery rate.

[0079] During the reciprocating motion of the moving block 11 along the length of the inclined block 10, the short shaft 17 moves synchronously; during the movement of the short shaft 17, the gear 34 moves synchronously; during the movement of the gear 34, the gear 34 rotates by meshing with the rack 35; during the rotation of the gear 34, the short shaft 17 rotates synchronously. Therefore, while the short shaft 17 reciprocates along the length of the inclined block 10, the short shaft 17 can also rotate.

[0080] During the movement of the short shaft 17, the first cam 18 moves synchronously. During the rotation of the first cam 18, when the protrusion of the first cam 18 abuts against the transverse rod 40, the transverse rod 40 drives the arc block 16 to move vertically upward, compressing the first spring 19. When the protrusion of the first cam 18 no longer abuts against the transverse rod 40, the transverse rod 40 resets under the action of the first spring 19, causing the arc block 16 to move vertically downward. Therefore, while the arc block 16 reciprocates along the length of the inclined block 10, the arc block 16 can also reciprocate vertically.

[0081] During the movement of the arc-shaped block 16, the second brush block 20 moves synchronously. Through the reciprocating movement of the second brush block 20 along the length of the inclined block 10 and the vertical reciprocating movement of the second brush block 20, on the one hand, when the second brush block 20 moves vertically, it can extend into the holes of the filter screen 6, causing the residue blocking the holes to move from bottom to top and eventually fall onto the surface of the filter screen 6, no longer blocking the holes of the filter screen 6; on the other hand, when the second brush block 20 moves reciprocating along the length of the inclined block 10, it can ensure that the holes at any position along the length of the filter screen 6 are completely cleaned, further ensuring that there is no residue blocking the holes of the filter screen 6, thereby improving the filtration capacity of the filter screen 6.

[0082] In summary, by cleaning the surface residue of the filter screen 6 with the first bristle block 7 and cleaning the residue inside the holes of the filter screen 6 with the second bristle block 20, the filter screen 6 is fully guaranteed not to be clogged due to the residue, further enhancing the filtration effect of the filter screen 6 and ensuring that the filter screen 6 is always in a highly efficient filtration state.

[0083] The purpose of the recycling tank 30 is to collect the water filtered by the filter cloth 12 and realize the secondary use of water. Then, through the reciprocating motion of the piston block 23, the water flows back into the piston cylinder 22 along the recycling pipe 31, so that the filtered water can be applied to the tissue block again under the action of the piston block 23, thereby improving the water utilization rate.

[0084] The above descriptions are merely embodiments of the present invention, and common knowledge such as specific technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solutions of the present invention, and these should also be considered within the scope of protection of the present invention. These modifications and improvements will not affect the effectiveness of the implementation of the present invention or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A stem cell preparation device, comprising a housing with an inlet and an outlet; characterized in that: It also includes a cleaning mechanism installed inside the box; the cleaning mechanism includes an inclined cylinder, a storage box with a feed hole at the top, a cleaning component installed inside the inclined cylinder, a feed component for intermittently introducing tissue blocks from the storage box into the inclined cylinder, and a water outlet component for draining water into the inclined cylinder; the inclined cylinder is fixedly connected to the box body, and a through hole is opened at one end of the inclined cylinder near the bottom of the box body, the through hole communicating with the outlet; the storage box is fixedly connected to the top of the box body, and the feed hole communicating with the inlet; the cleaning component includes a rotating shaft, a filter screen, several first brush bristle blocks, a filter hole opened at the bottom of the inclined cylinder, and a drive unit for driving the rotating shaft to rotate; The water outlet assembly includes a water tank, a piston cylinder, a piston block, a second cam, and a second spring; the water tank is fixedly connected to the housing; the piston cylinder is fixedly connected to the housing; the piston block is slidably connected to the piston cylinder; the second cam is fixedly connected to the rotating shaft and abuts against the piston block; the two ends of the second spring are respectively connected to the piston cylinder and the piston block; the piston cylinder is connected to an inlet pipe and an outlet pipe; the inlet pipe is connected to the water tank; the outlet pipe has several outlet holes; the inclined cylinder has several side holes, which communicate with the outlet holes and are located on the movement trajectory of the first brush block; The feeding assembly includes a discharge hole on the storage box, a baffle for sealing the discharge hole, and a top hole on the top of the inclined cylinder; the baffle is attached to the outer wall of the storage box and is fixed to the piston block; the discharge hole is located above the top hole and can communicate with the top hole.

2. The stem cell preparation device according to claim 1, characterized in that: Several blocking blocks are equidistantly arranged along the axial direction of the rotating shaft. The blocking blocks are located between two adjacent first brush blocks and are fixed to the rotating shaft. The two adjacent blocking blocks are staggered. The filter screen is fixed to the filter hole. Several first brush blocks are equidistantly arranged along the axial direction of the rotating shaft. The first brush blocks are fixed to the rotating shaft and are located on the water outlet path of the water outlet assembly. The filter screen is located on the movement trajectory of the first brush blocks.

3. The stem cell preparation device according to claim 2, characterized in that: It also includes a filtration section; the filtration section includes an inclined block, a moving block, a filter cloth, several cleaning blocks, filter holes opened on the bottom of the inclined block, and a motion unit for driving the moving block to reciprocate along the length of the inclined block; the inclined block is located directly below the inclined cylinder, the inclined block is fixedly connected to the inclined cylinder, and the end of the inclined block near the bottom of the box faces the outlet; the filter cloth is fixedly connected to the filter holes; several cleaning blocks are all fixedly connected to the moving block, and the end of the cleaning block away from the moving block is in contact with the filter cloth.

4. The stem cell preparation device according to claim 3, characterized in that: It also includes a linkage part set on the moving block; the linkage part includes a vertical block, an arc-shaped block, a short shaft, a first cam, a first spring, several second brush bristle blocks, a side hole opened on the vertical block, and a linkage unit for driving the short shaft to rotate; the vertical block is fixedly connected to the moving block; the arc-shaped block is vertically slidably connected to the side hole, and the arc-shaped block can move vertically within the side hole; the short shaft is rotatably connected to the vertical block; the first cam is fixedly connected to the short shaft, and the first cam abuts against the arc-shaped block; the two ends of the first spring are respectively connected to the side hole and the arc-shaped block; several second brush bristle blocks are all fixedly connected to the arc-shaped block, and the filter screen is located on the movement trajectory of the second brush bristle blocks.

5. The stem cell preparation device according to claim 4, characterized in that: It also includes a recycling section; the recycling section includes a recycling tank and a recycling pipe; the recycling tank is fixedly connected to the housing; one end of the recycling pipe is connected to the piston cylinder, and the other end of the recycling pipe extends into the recycling tank.

6. The stem cell preparation device according to claim 5, characterized in that: The motion unit includes a screw, a belt, and a side groove on the side wall of the inclined block; the screw is rotatably connected to the housing; the two ends of the belt are sleeved between the rotating shaft and the screw; the moving block is threadedly connected to the screw and slidably connected to the side groove.

7. The stem cell preparation device according to claim 6, characterized in that: The linkage unit includes a gear and a rack; the gear is fixedly connected to the short shaft; the rack is fixedly connected to the inclined block, and the gear and rack mesh.

8. A cleaning method for a stem cell preparation device according to claim 7, comprising the following steps, characterized in that: Step (1): Staff members place the tissue block into the storage box along the inlet for storage; Step (2): Through the reciprocating motion of the piston block, water enters the inclined cylinder along the drain pipe on the one hand, and tissue blocks intermittently enter the inclined cylinder along the top hole on the other hand. Step (3): Through the combined action of the first brush block and the blocking block, the time the tissue block stays in the inclined cylinder is extended on the one hand, and the foreign matter with strong adhesion on the surface of the tissue block is cleaned on the other hand. Step (4): By moving the block back and forth, the cleaning block cleans the foreign matter remaining on the surface of the filter cloth, and the second brush block cleans the foreign matter remaining in the holes of the filter screen. Step (5): Water recycling is achieved through the combined action of the recycling blocks and recycling pipes.

Images