A composition with auxiliary protective function for gastric mucosal damage and its application

Abstract

The present invention discloses a composition having an auxiliary protective function for gastric mucosal damage and its application, belonging to the application field of medicinal and edible compositions. The composition comprises the following components in parts by weight: 6 to 12 parts of ginseng, 18 to 30 parts of lotus seeds, and 60 to 90 parts of lotus roots. Based on the theory of traditional Chinese medicine, the present invention uses ginseng, lotus seeds, and lotus roots as raw materials, and the three are combined in proportion to have a significant synergistic effect in auxiliary protection of gastric mucosal damage. Animal experiments have shown that the composition of the present invention can reduce the MDA content in animal serum, increase SOD activity, have a protective effect on gastric mucosal damage, increase the NO and PGE2 content in animal gastric tissue, and reduce gastric mucosal damage. The composition of the present invention is of great significance for the development of health products for auxiliary protection of gastric mucosal damage and drugs for treating gastric mucosal damage.

Description

Technical Field

[0001] The present invention relates to the application field of a medicine-food composite, and in particular to a composite having an auxiliary protective function for gastric mucosal damage and an application thereof. Background Art

[0002] The stomach is an important digestive organ in the human body. It has the functions of storing, digesting and absorbing food, absorbing some nutrients, secreting gastric juice, gastrin, motilin and somatostatin, and preventing the invasion of pathogenic microorganisms and foreign bodies. Damage to the gastric mucosa can induce a variety of diseases, such as peptic ulcer, acute and chronic gastritis, gastric cancer, etc. Protecting the gastric mucosa is of great significance in preventing stomach-related diseases. At present, the commonly used clinical drugs are H + Pump inhibitors (prazoles), H2 receptor antagonists (tidines), gastric mucosal protectants (colloidal bismuth, sucralfate, etc.), and antacids (sodium bicarbonate, aluminum hydroxide) are usually used in combination. Long-term use of the above drugs has many side effects. Therefore, finding green, safe, and efficient health products or medicines is a common goal of those skilled in the art.

[0003] SOD, an antioxidant enzyme essential for the survival of aerobic cells, can scavenge oxygen free radicals produced during metabolism. As a product of oxygen free radicals, MDA can reflect the degree of lipid peroxidation in the body. NO is a messenger molecule and inflammatory mediator catalyzed by nitric oxide synthase (NOS). NOS plays an important role in gastric mucosal damage. The NO catalyzed by NOS participates in the secretion of gastric mucus and the regulation of gastric acid. It can dilate gastric mucosal blood vessels, maintain gastric mucosal integrity, and play a role in protecting the gastric mucosa. PGE2, as one of the main prostaglandins in the gastric mucosa, can improve gastric mucosal blood flow. Adequate gastric mucosal blood flow can not only provide abundant oxygen and nutrients to gastric mucosal cells, but also remove substances that damage the epithelial barrier, playing an important protective role in the integrity of the gastric mucosa. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition with auxiliary protection function for gastric mucosal damage and its application, so as to solve the problems existing in the above-mentioned prior art. The present invention uses ginseng, lotus seeds and lotus roots as raw materials, and the combination of the three has a significant synergistic effect on the auxiliary protection of gastric mucosal damage.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The invention provides a composition with auxiliary protective function for gastric mucosal damage. The composition comprises the following components in parts by weight: 6-12 parts of ginseng, 18-30 parts of lotus seeds and 60-90 parts of lotus roots.

[0007] Preferably, the composition comprises the following components in parts by weight: 6-12 parts of ginseng, 18-27 parts of lotus seeds and 70-90 parts of lotus roots.

[0008] More preferably, the composition comprises the following components in parts by weight: 9 parts of ginseng, 24 parts of lotus seeds and 75 parts of lotus roots.

[0009] In the above-mentioned composition, ginseng has the benefits of significantly replenishing vital energy, strengthening the spleen and lungs, promoting the production of body fluids and quenching thirst, and calming the mind and improving intelligence. Modern research shows that ginseng can reduce gastric acid secretion, reduce the release of inflammatory mediators, and alleviate gastric inflammatory responses, thereby protecting the gastric mucosa and enhancing its defenses. Furthermore, ginseng is rich in various components with antioxidant activity, such as ginsenosides and ginseng polysaccharides, which can scavenge free radicals, reduce oxidative damage, and protect the gastric mucosa from oxidative stress.

[0010] Lotus seeds have the therapeutic effects of tonifying the middle and replenishing qi, calming the mind, and nourishing the heart and kidneys. Modern research shows that lotus seed polysaccharides can mitigate gastric mucosal damage caused by nonsteroidal anti-inflammatory drugs. By inhibiting inflammation and enhancing the gastric mucosal repair capacity, they can alleviate gastric mucosal damage and inflammation, while protecting the integrity of the gastric mucosa. Furthermore, specific proteins in lotus seeds can enhance the defensive function of the gastric mucosa and increase the stability of the mucosal barrier, thereby reducing gastric acid damage to the gastric mucosa.

[0011] Lotus root has the benefits of clearing heat and moistening the lungs, nourishing yin and tonifying deficiency, and invigorating qi and blood. Modern research indicates that lotus root cores can protect against gastric mucosal damage and promote repair. This may be due to components such as mucus polysaccharides in the lotus root core. Mucus polysaccharides lubricate, protect, and repair the mucosa, have certain anti-ulcer properties, and help inhibit the secretion of acidic gastric juice. The polysaccharides in lotus root also possess significant antioxidant properties, reducing the production and scavenging of free radicals, alleviating oxidative damage and inflammatory responses in the gastric mucosa, thereby protecting the gastric mucosa.

[0012] The present invention also provides the use of the composition in preparing a health product with auxiliary protective function against gastric mucosal damage.

[0013] The present invention also provides a health product with auxiliary protective function for gastric mucosal damage, comprising the composition.

[0014] Preferably, the health care product further comprises excipients acceptable for health care products.

[0015] The present invention also provides the use of the composition in preparing a medicine for treating gastric mucosal damage.

[0016] The present invention also provides a medicine for treating gastric mucosal damage, comprising the composition.

[0017] Preferably, the drug further comprises a pharmaceutically acceptable excipient.

[0018] The present invention also provides a method for preparing the composition, comprising the following steps:

[0019] According to the dosage of each component of the composition, take each component of the composition;

[0020] The ginseng is removed from impurities, crushed, mixed with a 70% ethanol solution 8-15 times the mass of the ginseng, heated under reflux for 3-5 hours, condensed under reflux for 2-3 times to obtain a ginseng extract, and then concentrated and dried to obtain a ginseng extract;

[0021] removing the core of the lotus seeds, drying, crushing, and sieving to obtain lotus seed powder;

[0022] The lotus root is crushed, filtered, the filtrate is collected, allowed to stand to remove water, and dried at low temperature to obtain lotus root powder;

[0023] The ginseng extract, the lotus seed powder and the lotus root powder are fully mixed to obtain the composition.

[0024] The present invention discloses the following technical effects:

[0025] Based on traditional Chinese medicine theory, the present invention combines ginseng, lotus seeds, and lotus roots into a composition. Compared to any two of these ingredients combined, the combination of ginseng, lotus seeds, and lotus roots in a certain ratio exhibits a significant synergistic effect in assisting the protection of gastric mucosal damage. Animal experiments have shown that the disclosed composition of ginseng, lotus seeds, and lotus roots can reduce serum MDA levels and increase SOD activity in rats with a gastric mucosal damage model, demonstrating a protective effect against gastric mucosal damage. The composition can also increase the levels of NO and PGE2 in gastric tissue of rats with a gastric mucosal damage model, alleviating gastric mucosal damage. The composition of the present invention is of great significance for the development of health products that assist in the protection of gastric mucosal damage and drugs for the treatment of gastric mucosal damage. DETAILED DESCRIPTION

[0026] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0027] It should be understood that the terms described herein are intended only to describe particular embodiments and are not intended to limit the present invention. In addition, for numerical ranges herein, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value within any stated value or stated range, and each smaller range between any other stated value or intermediate value within the stated range, is also encompassed within the present invention. The upper and lower limits of these smaller ranges may be independently included or excluded within the scope.

[0028] Unless otherwise indicated, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of any conflict with any incorporated document, the contents of this specification shall prevail.

[0029] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from the description of the invention. The description and examples are intended to be illustrative only.

[0030] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0031] Example 1

[0032] 1. Preparation of the composition

[0033] 1.1 Raw materials

[0034] According to the formula requirements of each group in Example 2.1.2, the corresponding weight portions of ginseng, lotus seeds and lotus roots were weighed respectively. Unless otherwise specified, the "parts" mentioned in the present invention are all calculated by weight.

[0035] 1.2 Preparation method

[0036] Ginseng processing method: remove impurities from dried ginseng, crush it, pass it through a 200-mesh sieve, add a 70% ethanol solution 10 times the mass of the ginseng, heat and reflux extract for 4 hours, then condense and reflux extract twice, concentrate the obtained extract to a viscous extract state, and dry it to obtain the ginseng extract;

[0037] Lotus seed processing method: remove the core of the lotus seeds, dry them at 125°C, grind them, and pass them through a 200-mesh sieve to obtain lotus seed powder;

[0038] The lotus root processing method is as follows: the lotus root is crushed with a wall breaking machine, filtered, the filtrate is collected, allowed to stand to remove the clear water, and dried at low temperature to obtain lotus root powder;

[0039] The ginseng extract, lotus seed powder and lotus root powder obtained above are fully mixed to prepare a composition.

[0040] 2. Animal Experiments

[0041] 2.1 Materials and Methods

[0042] 2.1.1 Experimental animals

[0043] SD rats, body weight 180-200 g.

[0044] 2.1.2 Grouping and Dosage Method

[0045] After 7 days of adaptive feeding, the animals were randomly divided into six groups, including a blank control group, a model group, and four test sample groups: Group A, Group B, Group C, and Group D. The blank control group and the model group were given normal saline, while the four test sample groups (Group A, Group B, Group C, and Group D) were given a dose of 1.71 g / kg·bw (bw represents the body weight of the test animals) for 30 consecutive days. The general condition of the rats was observed and recorded once a day.

[0046] The test samples in group A consisted of 9 parts of ginseng, 24 parts of lotus seeds and 75 parts of lotus roots;

[0047] The test samples of group B consisted of 9 parts of ginseng and 24 parts of lotus seeds;

[0048] The test samples in group C consisted of 9 parts of ginseng and 75 parts of lotus root;

[0049] The test samples in group D consisted of 24 parts of lotus seeds and 75 parts of lotus roots;

[0050] 2.1.3 Observation and statistics of gastric mucosal damage

[0051] After the last administration, all rats were fasted but not watered for 24 hours. Except for the blank control group, all rats were given 1.0 mL of anhydrous ethanol. After 1 hour, the rats were killed and blood was collected for later use. The entire stomach was exposed, the pylorus was ligated, and a 10% formaldehyde aqueous solution was perfused and fixed for 20 minutes. Then, the stomach was cut open along the greater curvature, the stomach contents were washed, the gastric mucosa was unfolded, and the length and width of the bleeding point or bleeding band were measured with a vernier caliper under a stereoscopic dissecting microscope. According to the observation results, the scoring criteria are shown in Table 1. The total score was calculated, and the injury incidence (%), injury score index and injury inhibition rate (%) were calculated according to the following formula:

[0052] Injury incidence (%) = number of rats with bleeding or ulceration in a certain tissue / number of rats in the group × 100%;

[0053] Damage score index = total damage score of each group / number of animals in each group;

[0054] Damage inhibition rate (%) = (damage score of model group - damage score of test substance group) / damage score of model group × 100%.

[0055] Table 1 Gross observation scoring criteria for acute anhydrous ethanol injury

[0056]

[0057] 2.1.4 Detection of gastric mucosal injury indicators

[0058] The superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, nitric oxide (NO) content and prostaglandin E2 (PGE2) content in the serum of rats in the blank control group, model group, group A, group B, group C and group D were detected according to conventional methods.

[0059] 2.2 Experimental Results

[0060] 2.2.1 Effects of different compositions on gastric mucosal injury in rats

[0061] The results are shown in Table 2. After anhydrous ethanol modeling, the gastric mucosal injury rate of rats was 100%. Compared with the model group, the gastric mucosal congestion area and injury index of rats in each drug-treated group were reduced. Among them, the gastric mucosal injury integral index of rats in Group A was the smallest, and the injury inhibition rate was the highest, reaching 58.79%. This shows that the composition in Group A has the strongest gastric mucosal injury protection ability.

[0062] Table 2 Effects of various compositions on acute gastric mucosal injury in rats

[0063]

[0064] Note: Compared with the model group: **P<0.01; ***P<0.001.

[0065] 2.2.2 Effects of different compositions on gastric mucosal injury indicators in rats

[0066] The results are shown in Table 3. Compared with the blank control group, the serum MDA level of the model group rats was significantly increased (P < 0.01), and the SOD level was significantly decreased (P < 0.01). Compared with the model group, the SOD activity of groups A, B, and C increased, and the MDA content decreased. The effect of group A was better than that of groups B and C, and group D showed a regulatory trend, but it was not statistically significant (P > 0.05). Compared with the blank control group, the serum NO level of the model group rats was significantly decreased (P < 0.01), and the PGE2 level was significantly decreased (P < 0.01). Compared with the model group, the NO level of group A was significantly increased, and the PGE2 level was significantly increased (P < 0.01). The effect of group A was better. Only the NO level of group B was significantly increased, and only the PGE2 level of group C was significantly increased. Group D showed a regulatory trend, but it was not statistically significant (P > 0.05).

[0067] Table 3 Effects of various compositions on acute gastric mucosal injury in rats

[0068]

[0069] Note: Compared with the blank control group: #P<0.05, ##P<0.01; compared with the model group: *P<0.05; **P<0.01.

[0070] Example 2

[0071] The difference from Example 1 is that the raw materials of the composition are 6 parts of ginseng, 30 parts of lotus seeds, and 60 parts of lotus roots.

[0072] Example 3

[0073] The difference from Example 1 is that the raw materials of the composition are 12 parts of ginseng, 18 parts of lotus seeds and 90 parts of lotus roots.

[0074] Example 4

[0075] The difference from Example 1 is that the raw materials of the composition are 9 parts of ginseng, 27 parts of lotus seeds and 70 parts of lotus roots.

[0076] Example 5

[0077] The difference from Example 1 is that the raw materials of the composition are 12 parts of ginseng, 21 parts of lotus seeds and 80 parts of lotus roots.

[0078] Comparative Example 1

[0079] The difference from Example 1 is that the raw materials of the composition are 5 parts of ginseng, 15 parts of lotus seeds and 66 parts of lotus roots.

[0080] Comparative Example 2

[0081] The difference from Example 1 is that the raw materials of the composition are 9 parts of ginseng, 18 parts of lotus seeds and 55 parts of lotus roots.

[0082] The experimental results of the compositions of Examples 2-5 and Comparative Examples 1-2 on gastric mucosal injury in rats are summarized in Table 4, and the experimental results of the effects of the compositions of Examples 2-5 and Comparative Examples 1-2 on gastric mucosal injury indicators in rats are summarized in Table 5.

[0083] Table 4 Effects of each composition on acute gastric mucosal injury in rats

[0084]

[0085] Note: Compared with the model group: **P<0.01; ***P<0.001.

[0086] Table 5 Effects of various compositions on acute gastric mucosal injury in rats

[0087]

[0088]

[0089] Note: Compared with the blank control group: #P<0.05, ##P<0.01; compared with the model group: *P<0.05, **P<0.01.

[0090] As can be seen from Tables 4 and 5, compared with the model group, the compositions prepared in Examples 2-5 can significantly reduce the gastric mucosal injury score index of rats, improve the injury inhibition rate, and at the same time significantly enhance the SOD activity and reduce the MDA content, and significantly enhance the NO and PGE2 contents; the results of the various test indicators of the comparative examples 1-2 groups were not significantly different from those of the model group, indicating that only the composition prepared within the ratio range of 6-12 parts of ginseng, 18-30 parts of lotus seeds and 60-90 parts of lotus roots has a good auxiliary effect in protecting gastric mucosal damage.

[0091] The embodiments described above are merely descriptions of preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Without departing from the spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by persons skilled in the art should fall within the scope of protection defined by the claims of the present invention.

Claims

1. A composition having an auxiliary protective function against gastric mucosal damage, characterized in that: The composition consists of the following components in parts by weight: 6-12 parts of ginseng, 18-30 parts of lotus seeds and 60-90 parts of lotus roots.

2. The composition according to claim 1, wherein The composition consists of the following components in parts by weight: 6-12 parts of ginseng, 18-27 parts of lotus seeds and 70-90 parts of lotus roots.

3. The composition according to claim 2, wherein The composition consists of the following components in parts by weight: 9 parts of ginseng, 24 parts of lotus seeds and 75 parts of lotus roots.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of a health product having an auxiliary protective function against gastric mucosal damage.

5. A health product with auxiliary protective function for gastric mucosal damage, characterized in that: The invention comprises the composition according to any one of claims 1 to 3.

6. The health care product according to claim 5, characterized in that The health care product further comprises excipients acceptable to health care products.

7. Use of the composition according to any one of claims 1 to 3 in the preparation of a medicament for treating gastric mucosal damage.

8. A drug for treating gastric mucosal damage, characterized in that: The invention comprises the composition according to any one of claims 1 to 3.

9. The drug according to claim 8, wherein The drug also includes pharmaceutically acceptable excipients.

10. A method for preparing the composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: According to the dosage of each component of the composition, take each component of the composition; The ginseng is removed from impurities, crushed, mixed with a 70% ethanol solution 8-15 times the mass of the ginseng, heated under reflux for 3-5 hours, condensed under reflux for 2-3 times to obtain a ginseng extract, and then concentrated and dried to obtain a ginseng extract; removing the core of the lotus seeds, drying, crushing, and sieving to obtain lotus seed powder; The lotus root is crushed, filtered, the filtrate is collected, allowed to stand to remove water, and dried at low temperature to obtain lotus root powder; The ginseng extract, the lotus seed powder and the lotus root powder are fully mixed to obtain the composition.

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