Preparation method of moonwort extract, product and application thereof

Abstract

The present invention relates to a preparation method of a moonflower extract, and its product and application. The preparation method comprises the following steps: heating and leaching the moonflower raw material with an ionic liquid aqueous solution as an extractant, centrifuging the extract, and filtering the supernatant under reduced pressure to obtain the ionic liquid, wherein the ionic liquid is prepared with a combination of betaine, glycerol and levulinic acid as raw materials. The extract prepared by the preparation method has a high total flavonoid content, short time consumption and low cost, and the extractant is a common cosmetic raw material, and there is no organic solvent residue, thereby avoiding the risk of using flammable and explosive substances such as ethanol. The moonflower extract prepared by the preparation method has an outstanding antioxidant effect, and at the same time has multiple effects of soothing, whitening and moisturizing. It can be used in cosmetics to delay skin aging, inhibit the synthesis of melanin in the skin, and keep the skin bright and translucent.

Description

Technical Field

[0001] The invention belongs to the technical field of plant extraction, and relates to a preparation method of a melaleuca alternifolia extract, a product and an application thereof. Background Art

[0002] In recent years, due to the increasing pressure of life and the increased intake of high-calorie, high-protein, and high-fat foods, the internal environment of the human body has become unbalanced. Coupled with excessive exposure to ultraviolet rays and stimulation by harmful substances in the external environment, more skin problems have occurred, such as dry skin, dullness, dark spots, etc., which has brought great psychological pressure to people.

[0003] At present, ascorbic acid, kojic acid, arbutin and their derivatives, or extracts with tyrosinase inhibitory activity have been used in cosmetics or medicines to improve the common problem of dull skin. However, their application is greatly limited due to the insufficient skin care effect of a single ingredient, instability in cosmetics, low extraction rate of some active ingredients, and unclear skin care effect.

[0004] Moonflower, also known as white-backed Panax notoginseng, white-seed vegetable, and fairy grass, belongs to the Asteraceae family and the genus Panax notoginseng. It is a dicotyledonous plant and a perennial evergreen plant. Moonflower is rich in flavonoids, including the unique ingredient chalcones. The flavonoids in moonflower have antioxidant and blood sugar-lowering biological activities, and can also reduce the total cholesterol and triglyceride content in animals. It is known as a healthy food for humans in the 21st century.

[0005] At present, there are many studies on obtaining flavonoids from plants, such as hot water extraction and alcohol-water extraction. Conventional solvent extraction methods have problems such as low extraction rate and many impurities. As for the method of extracting total flavonoids from moonflower, the research content is relatively simple, and there is no report on the extraction process that takes into account both improving extraction efficiency and facilitating cosmetic application. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a method for preparing a melaleuca extract and its product and application.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides a method for preparing a moonflower extract, the preparation method comprising the following steps:

[0009] (1) heating and extracting the raw material of moonwort with an ionic liquid aqueous solution as an extractant to obtain an extract;

[0010] (2) centrifuging the extract, taking the supernatant and filtering it under reduced pressure to obtain a moonflower extract;

[0011] Wherein, the ionic liquid in step (1) is prepared using a combination of betaine, glycerol and levulinic acid as raw materials.

[0012] The invention relates to a method for preparing a moonflower extract, which creatively uses an aqueous solution of an ionic liquid prepared from betaine, glycerol and levulinic acid as raw materials as an extractant for heating and leaching, and utilizes a low eutectic solvent extraction method. On the one hand, betaine is used as a hydrogen bond acceptor, and glycerol and levulinic acid are used as hydrogen bond donors. The total flavonoid content in the moonflower extract is improved by utilizing a combination of the three. The method is time-saving, low-cost, and realizes factory-scale large-scale production. At the same time, the low eutectic solvent selected is a common cosmetic raw material, and while there is no organic solvent residue, the risk of using flammable and explosive substances such as ethanol is avoided. The extraction process has high safety and extraction efficiency.

[0013] Preferably, the mass ratio of betaine, glycerol and levulinic acid is (2-4):(8-12):1, wherein the specific point values ​​in (2-4) can be independently selected from 2, 2.1, 2.2, 2.4, 2.7, 3, 3.3, 3.6, 4, etc., and the specific point values ​​in (8-12) can be independently selected from 8, 8.2, 8.4, 8.7, 9.0, 9.5, 10.0, 10.5, 11.0, 11.5, 12, etc. Other specific point values ​​within the above numerical range can be selected and will not be described one by one here.

[0014] The present invention creatively finds that when an aqueous solution of an ionic liquid prepared by using betaine, glycerol and levulinic acid in a specific mass ratio as raw materials is used as an extractant, the total flavonoids content in the prepared moonwort extract is significantly increased and has more excellent antioxidant properties.

[0015] Preferably, the preparation of the ionic liquid is carried out at 40-80°C (e.g., 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C) until the system becomes a clear and transparent liquid. Other specific point values ​​within this numerical range can be selected and will not be described one by one here.

[0016] Preferably, the moonflower raw material in step (1) is moonflower powder that is crushed and sieved through 20-100 mesh (for example, 20 mesh, 25 mesh, 30 mesh, 35 mesh, 40 mesh, 45 mesh, 50 mesh, 60 mesh, 70 mesh, 80 mesh, 90 mesh, 100 mesh, etc.). Other specific point values ​​within this numerical range can be selected, and they will not be described here one by one.

[0017] Preferably, the ionic liquid aqueous solution is an ionic liquid aqueous solution with a mass fraction of 40-80% (e.g., 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%), and other specific point values ​​within this numerical range can be selected, which will not be described one by one here.

[0018] The present invention creatively finds that the concentration of ionic liquid will also affect the content of total flavonoids in the moonflower extract. The total flavonoids content in the moonflower extract prepared by using 40-80% ionic liquid aqueous solution is significantly improved, and has more excellent antioxidant properties.

[0019] Preferably, the dosage ratio of the moonflower raw material to the extractant in step (1) is 1g:(10-30)mL, wherein the specific point values ​​in (10-30) can be independently selected as 10, 11, 12, 14, 17, 20, 23, 26, 30, etc., and other specific point values ​​within this numerical range can be selected, which will not be repeated here.

[0020] Preferably, the heating extraction in step (1) is carried out at 60-90°C (for example, 60°C, 62°C, 64°C, 66°C, 68°C, 70°C, 75°C, 80°C, 85°C, 90°C, etc.), and the heating extraction time is 0.5-2.5h, for example, 0.5h, 0.7h, 0.9h, 1.1h, 1.3h, 1.5h, 1.7h, 2.0h, 2.3h, 2.5h, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be repeated here one by one.

[0021] Preferably, the centrifugal speed in step (2) is 5000-10000rpm, for example, 5000rpm, 5100rpm, 5300rpm, 5700rpm, 6000rpm, 6500rpm, 7000rpm, 8000rpm, 9000rpm, 10000rpm, etc., and the centrifugal time is 5-10min, for example, 5min, 6min, 7min, 8min, 9min, 10min, etc. Other specific point values ​​within the above numerical range can be selected, and they will not be described one by one here.

[0022] Preferably, the pore size of the filter membrane for the reduced pressure filtration in step (2) is not greater than 5 μm, for example 5 μm, 4.5 μm, 4 μm, 3 μm, 2 μm, 1 μm, etc. Other specific point values ​​within this numerical range can be selected and will not be described one by one here.

[0023] Preferably, the preparation method comprises the following steps:

[0024] The raw material of moonflower is crushed and passed through a 20-100 mesh sieve, and the moonflower powder is extracted with a 40-80% mass fraction ionic liquid aqueous solution at 60-90°C for 0.5-2.5h, wherein the ionic liquid is prepared with betaine, glycerol and levulinic acid as raw materials in a mass ratio of (2-4):(8-12):1 to obtain an extract, and the extract is centrifuged at 5000-10000rpm for 5-10min to obtain a supernatant, and the supernatant is filtered under reduced pressure using a filter membrane with a pore size of no more than 5μm to obtain a moonflower extract.

[0025] In a second aspect, the present invention provides a moonflower extract obtained by the preparation method of the moonflower extract described in the first aspect.

[0026] The moonflower extract prepared by the present invention contains multiple active ingredients such as flavonoids, polyphenols, polysaccharides, etc., which act synergistically to make the moonflower extract have significant antioxidant activity, and also have the performance of inhibiting tyrosinase activity and skin moisturizing.

[0027] In a third aspect, the present invention provides a use of the moonflower extract according to the second aspect in the preparation of cosmetics.

[0028] The present invention creatively applies the moonflower extract to cosmetics, which can delay skin aging and inhibit the synthesis of melanin in the skin, thus keeping the skin bright and translucent.

[0029] Preferably, the content of moonflower extract in the cosmetic is 0.01-1% by weight, for example 0.01%, 0.02%, 0.05%, 0.08%, 1%, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0030] Preferably, the cosmetics include essence, lotion, emulsion, cream, mask or cleanser.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The preparation method of the moonflower extract involved in the present invention creatively adopts an ionic liquid aqueous solution composed of betaine, glycerol and levulinic acid for heating and leaching, and uses a low eutectic solvent extraction method. On the one hand, the synergistic effect between betaine, glycerol and levulinic acid is utilized to increase the total flavonoid content in the moonflower extract, which is time-consuming, low-cost, and realizes factory-scale production. At the same time, the selected low eutectic solvent is a common cosmetic raw material, and there is no organic solvent residue, while also avoiding the risk of using flammable and explosive substances such as ethanol. The extraction process is highly safe and has high extraction efficiency. The present invention creatively uses 40-80% of ionic liquid aqueous solution as an extractant to obtain a significantly increased total flavonoid content in the moonflower extract, and has more excellent antioxidant properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is a graph showing the inhibition rate of tyrosinase activity of the moonflower extract obtained in Example 1 at different dilution concentrations;

[0034] Figure 2 The cytotoxicity graph of the moonflower extract prepared in Example 1 at different dilution concentrations. DETAILED DESCRIPTION

[0035] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0036] In the following examples, unless otherwise specified, the reagents and consumables used were purchased from conventional reagent manufacturers in the field; unless otherwise specified, the experimental methods and technical means used were conventional methods and means in the field.

[0037] Example 1

[0038] This embodiment provides a method for preparing a moonflower extract:

[0039] (1) betaine, glycerol and levulinic acid in a mass ratio of 3:10:1 were weighed and heated at 60° C. until the system became a clear and transparent liquid to obtain an ionic liquid;

[0040] (2) Weigh 50 g of moonflower powder that has passed through an 80-mesh sieve, add 1000 mL of an aqueous solution of the ionic liquid prepared in step (1) with a mass fraction of 60% in a ratio of 1 g:20 mL of moonflower powder to extractant, and extract in a 75° C. water bath for 1.5 h to obtain an extract;

[0041] (2) The extract obtained in step (1) was centrifuged at 8000 rpm for 7.5 min, the supernatant was separated, and filtered with a 5 μm filter membrane to obtain a moonflower extract.

[0042] Example 2

[0043] This embodiment provides a method for preparing a moonflower extract:

[0044] (1) betaine, glycerol and levulinic acid in a mass ratio of 4:8:1 were weighed and heated at 50° C. until the system became a clear and transparent liquid to obtain an ionic liquid;

[0045] (2) Weigh 50 g of moonflower powder that has passed through a 100-mesh sieve, add 500 mL of an aqueous solution of the ionic liquid prepared in step (1) with a mass fraction of 80% in a ratio of 1 g:10 mL of moonflower powder to extractant, and extract in a 60° C. water bath for 2.5 h to obtain an extract;

[0046] (3) The extract obtained in step (1) was centrifuged at 5000 rpm for 10 min, the supernatant was separated, and filtered with a 5 μm filter membrane to obtain a moonflower extract.

[0047] Example 3

[0048] This embodiment provides a method for preparing a moonflower extract:

[0049] (1) betaine, glycerol and levulinic acid in a mass ratio of 2:12:1 were weighed and heated at 70° C. until the system became a clear and transparent liquid to obtain an ionic liquid;

[0050] (2) Weigh 50 g of moonflower powder that has passed through a 60-mesh sieve, add 1500 mL of a 40% aqueous solution of the ionic liquid prepared in step (1) in a ratio of 1 g:30 mL of moonflower powder to extractant, and extract in a 90° C. water bath for 0.5 h to obtain an extract;

[0051] (3) The extract obtained in step (1) was centrifuged at 10,000 rpm for 5 min, the supernatant was separated, and filtered with a 5 μm filter membrane to obtain a moonflower extract.

[0052] Example 4

[0053] This embodiment provides a method for preparing a moonflower extract, which is different from Embodiment 1 only in that the mass ratio of betaine, glycerol and levulinic acid in step (1) is 3:7:4, and other conditions remain unchanged.

[0054] Example 5

[0055] This embodiment provides a method for preparing a moonflower extract, which differs from Embodiment 1 only in that the mass ratio of betaine, glycerol and levulinic acid in step (1) is 3:10.3:0.7, and other conditions remain unchanged.

[0056] Example 6

[0057] This embodiment provides a method for preparing a moonflower extract, which differs from Embodiment 1 only in that the extractant in step (2) is an ionic liquid aqueous solution with a mass fraction of 30%, and other conditions remain unchanged.

[0058] Example 7

[0059] This embodiment provides a method for preparing a moonflower extract, which differs from Embodiment 1 only in that the extractant in step (2) is an ionic liquid aqueous solution with a mass fraction of 90%, and other conditions remain unchanged.

[0060] Comparative Example 1

[0061] This comparative example provides a method for preparing a moonwort extract, which differs from Example 1 only in that in step (1), betaine and glycerol in a mass ratio of 3:11 are weighed and heated at 60° C. until the system becomes a clear and transparent liquid to obtain an ionic liquid, and other conditions remain unchanged.

[0062] Comparative Example 2

[0063] This comparative example provides a method for preparing a moonwort extract, which differs from Example 1 only in that in step (1), betaine and levulinic acid in a mass ratio of 3:11 are weighed and heated at 60° C. until the system becomes a clear and transparent liquid to obtain an ionic liquid, and other conditions remain unchanged.

[0064] Comparative Example 3

[0065] This comparative example provides a method for preparing a moonflower extract, which differs from Example 1 only in that in step (1), betaine, glycerol and propylene glycol in a mass ratio of 3:10:1 are weighed and heated at 60° C. until the system becomes a clear and transparent liquid to obtain an ionic liquid, and other conditions remain unchanged.

[0066] Comparative Example 4

[0067] This comparative example provides a method for preparing a moonwort extract, which differs from Example 1 only in that, in step (1), choline chloride, glycerol and levulinic acid in a mass ratio of 3:10:1 are weighed and heated at 60° C. until the system becomes a clear and transparent liquid to obtain an ionic liquid, and other conditions remain unchanged.

[0068] Comparative Example 5

[0069] This comparative example provides a method for preparing a moonflower extract, which differs from Example 1 only in that the extracting agent is replaced with deionized water in equal amounts, and other conditions remain unchanged.

[0070] Comparative Example 6

[0071] This comparative example provides a method for preparing a moonwort extract, which differs from Example 1 only in that the extracting agent is replaced with an equal amount of 70% ethanol aqueous solution, and other conditions remain unchanged.

[0072] Test Example 1

[0073] The total flavonoid content of the moonflower extracts obtained in Examples 1-7 and Comparative Examples 1-6 was determined by the following method:

[0074] (1) Sample pretreatment: Take about 1 mL of the moonflower extract sample and filter it through a 0.45 μm filter membrane for later use;

[0075] (2) Reagent preparation:

[0076] Standard stock solution: Accurately weigh 10 mg of rutin standard, dissolve it in anhydrous ethanol, and dilute to 50 mL to obtain a 0.2 mg / mL standard stock solution;

[0077] 5% sodium nitrite NaNO2 solution: accurately weigh 5g NaNO2, add water to 100g, and stir to dissolve;

[0078] 10% aluminum nitrate Al(NO3)3·9H2O solution: accurately weigh 10g Al(NO3)3·9H2O, add water to 100g, and stir to dissolve;

[0079] 4% NaOH solution: weigh 4g NaOH, add water to 100g, and stir to dissolve;

[0080] (3) Preparation of standard curve:

[0081] Take 0mL, 0.4mL, 0.8mL, 1.2mL, 1.6mL, 2.0mL, 2.4mL of standard stock solution respectively into 10mL stoppered graduated test tubes, add 2.4mL, 2.0mL, 1.6mL, 1.2mL, 0.8mL, 0.4mL, 0mL of water respectively (i.e. add water to 2.4mL each), then add 0.4mL of 5% NaNO2 solution respectively, shake and mix, and let stand for 6min; then add 0.4mL of 10% Al(NO3)3 solution, shake and mix, and let stand for 6min; then add 4.0mL of 4% NaOH solution, add water to 10mL (add 2.8mL), shake and mix, let stand for 15min, adjust to zero with the corresponding solvent blank, and measure the absorbance at 510nm. Draw a standard curve with rutin concentration as the horizontal axis and absorbance at 510nm as the vertical axis;

[0082] (4) Sample determination:

[0083] The sample solution was diluted in an appropriate ratio, and 2.4 mL of the sample was taken for determination. The experimental steps were the same as above, and three parallel samplings were performed for each sample.

[0084] The calculation formula of total flavonoids content in samples is as follows:

[0085] Total flavonoid content of sample (mg / mL) = (CX×10) / V×K×0.001

[0086] Where: the value 10 is the test volume mL, 0.001 is the unit conversion,

[0087] CX: The concentration calculated by substituting the absorbance value into the standard curve, μg / mL,

[0088] V: The volume of the sample to be tested, mL.

[0089] K: sample dilution multiple.

[0090] The measurement results are shown in Table 1:

[0091] Table 1

[0092] Group Total flavonoids content (mg / mL) Total flavonoid yield (%) Example 1 6.48 12.96 Example 2 11.36 11.36 Example 3 4.04 12.12 Example 4 5.21 10.41 Example 5 5.17 10.34 Example 6 4.28 8.55 Example 7 4.51 9.01 Comparative Example 1 3.52 7.04 Comparative Example 2 4.08 8.16 Comparative Example 3 4.93 9.86 Comparative Example 4 4.06 8.12 Comparative Example 5 2.21 4.42 Comparative Example 6 4.54 9.07

[0093] From the data in Table 1, it can be seen that by comparing Example 1 with Examples 4-5, it can be found that the mass ratio of betaine, glycerol and levulinic acid will have a significant effect on the yield of the extract and the content of total flavonoids; by comparing Example 1 with Examples 6-7, it can be found that the concentration of the ionic liquid will also affect the yield of the extract and the content of total flavonoids; by comparing Example 1 with Comparative Examples 1-2, it can be found that glycerol and levulinic acid, as hydrogen bond donors, play a synergistic role in the extraction of moonflower; by comparing Example 1 with Comparative Examples 3-6, it can be found that the combination of betaine, glycerol and levulinic acid, compared with using other ionic liquids, deionized water or ethanol aqueous solution as an extractant, can significantly improve the yield of the extract and the content of total flavonoids.

[0094] Test Example 2

[0095] The antioxidant properties of the moonflower extracts obtained in Examples 1-7 and Comparative Examples 1-6 were measured by the following method:

[0096] To avoid the difference in the dilution multiple of the extract concentration caused by the difference in the material-liquid ratio during the extraction of the moonflower extract in each embodiment and comparative example. The moonflower extract was diluted to a certain concentration according to the dilution multiple in Table 2, and the ability of the moonflower extract to scavenge DPPH free radicals at this concentration was tested. 2 mL of sample dilution and 2 mL of DPPH solution were respectively taken in a stoppered test tube, mixed, reacted in the dark for 30 minutes, and the absorbance value A1 at a wavelength of 517 nm was measured; 2 mL of sample solution and 2 mL of methanol were respectively taken in a stoppered test tube, mixed, reacted in the dark for 30 minutes, and the absorbance value A2 at a wavelength of 517 nm was measured; 2 mL of DPPH solution and 2 mL of methanol were respectively taken in a stoppered test tube, mixed, reacted in the dark for 30 minutes, and the absorbance value A0 at a wavelength of 517 nm was measured; 3 parallel samples were made for each sample.

[0097] Calculation formula:

[0098] The measurement results are shown in Table 2:

[0099] Table 2

[0100]

[0101]

[0102] It can be seen from the data in Table 2 that the moonflower extract prepared by the preparation method of the present invention has significant DPPH free radical scavenging ability. The selection of the extractant, especially the concentration and composition of the ionic liquid, will also significantly affect the DPPH free radical scavenging ability of the prepared extract.

[0103] Test Example 3

[0104] The tyrosinase activity inhibition test was performed on the moonflower extract prepared in Example 1. The determination method was based on T / SHRH 015-2018 Cosmetics-Tyrosinase Activity Inhibition Test Method. The absorbance value can be measured at 475nm on a spectrophotometer. According to the change in the absorbance value, the effect of different dilution concentrations of the moonflower extract prepared in Example 1 on the tyrosinase activity was evaluated. The results are as follows: Figure 1 As shown in the results, the extract of Herba Lycopodii has an excellent tyrosinase inhibitory effect, and its effect is concentration-dependent.

[0105] Test Example 4

[0106] The hyaluronidase activity inhibition test was performed on the moonflower extract prepared in Example 1. The determination method was based on T / JSRH 003-2023 Cosmetic Raw Material Anti-allergic Activity Test-Hyaluronidase Inhibition Method. By measuring the change in absorbance, the hyaluronidase inhibition rate of the sample was calculated, and the anti-allergic activity of the sample could be evaluated. The moonflower extract in Example 1 was diluted to 1%, and its hyaluronidase inhibition rate was measured to be 58.14%.

[0107] Test Example 5

[0108] The cytotoxicity test of the moonflower extract prepared in Example 1 was carried out. The determination method was based on GB / T16886.5-2017 Biological Evaluation of Medical Devices Part 5: In vitro Cytotoxicity Test. The experiment used RAW264.7 cells. The moonflower extract prepared in Example 1 was diluted according to different concentration ratios and added to DMEM culture medium (containing 2% fetal bovine serum and 1% double antibody). After mixing evenly, it was cultured and tested. The absorbance was measured at 450nm, and the cell survival rate was calculated. The results are as follows: Figure 2 As shown, RAW cells had a higher survival rate at different dilution concentrations of the moonflower extract.

[0109] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0110] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0111] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A method for preparing a moonflower extract, characterized in that: The preparation method comprises the following steps: (1) heating and extracting the raw material of moonwort with an ionic liquid aqueous solution as an extractant to obtain an extract; (2) centrifuging the extract, taking the supernatant and filtering it under reduced pressure to obtain a moonflower extract; The ionic liquid aqueous solution in step (1) is an ionic liquid aqueous solution with a mass fraction of 40-80%; the ionic liquid is prepared using a combination of betaine, glycerol and levulinic acid as raw materials; and the mass ratio of betaine, glycerol and levulinic acid is (2-4):(8-12):

1.

2. The method for preparing the moonflower extract according to claim 1, characterized in that: The preparation of the ionic liquid is carried out at 40-80° C. until the system becomes a clear and transparent liquid.

3. The method for preparing the moonflower extract according to claim 1, characterized in that: The moonflower raw material in step (1) is moonflower powder that is crushed and passed through a 20-100 mesh sieve.

4. The method for preparing the moonflower extract according to claim 1, characterized in that: In step (1), the dosage ratio of the moonflower raw material to the extractant is 1 g: (10-30) mL.

5. The method for preparing the moonflower extract according to claim 1, characterized in that: The heating leaching in step (1) is carried out at 60-90° C., and the heating leaching time is 0.5-2.5 h.

6. The method for preparing the moonflower extract according to claim 1, characterized in that: The centrifugal speed in step (2) is 5000-10000 rpm, and the centrifugal time is 5-10 min.

7. The method for preparing the moonflower extract according to claim 1, characterized in that: The pore size of the filter membrane used for the reduced pressure filtration in step (2) is no greater than 5 μm.

8. The method for preparing the moonflower extract according to claim 1, characterized in that: The preparation method comprises the following steps: The raw material of moonflower is crushed and passed through a 20-100 mesh sieve, and the moonflower powder is extracted with a 40-80% mass fraction ionic liquid aqueous solution at 60-90°C for 0.5-2.5 h to obtain an extract, wherein the ionic liquid is prepared with betaine, glycerol and levulinic acid as raw materials in a mass ratio of (2-4):(8-12):1, and the extract is centrifuged at 5000-10000 rpm for 5-10 min to obtain a supernatant, and the supernatant is filtered under reduced pressure using a filter membrane with a pore size of no more than 5 μm to obtain a moonflower extract.

9. A Herba Lycopodii extract obtained according to the preparation method of the Herba Lycopodii extract according to any one of claims 1 to 8.

10. Use of the moonflower extract according to claim 9 in preparing cosmetics.

11. The use of the moonflower extract according to claim 10 in preparing cosmetics, characterized in that: The content of the moonflower extract in the cosmetic is 0.01-1% by weight.

12. The use of the moonflower extract according to claim 10 in preparing cosmetics, characterized in that: The cosmetics include essence, lotion, emulsion, cream, facial mask or facial cleanser.

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