A composition for reducing skin problems around the eyes, and its preparation process and application

Abstract

The present invention discloses a composition for reducing skin problems around the eyes, a preparation process and an application thereof, wherein the composition comprises the following raw materials in parts by weight: 5-15 parts of Cambodian garcinia cambogia extract, 10-20 parts of horse chestnut extract, 5-15 parts of lactobionic acid, 0.5-5 parts of arginine, and water is added to make up to 100 parts. A preparation process of a composition for reducing skin problems around the eyes. An application of a composition for reducing skin problems around the eyes, which is applied to medicines or eye care products. The present invention has multiple effects of reducing dark circles, effectively removing eye bags, tightening and refreshing eye skin, etc., is mild and non-irritating while effectively preventing the formation of fat particles, and comprehensively protecting the eye skin.

Description

Technical Field

[0001] The present invention relates to a composition for eye protection, a preparation process and an application thereof, and in particular to a composition for reducing problems of skin around eyes such as dark circles, eye bags, eye swelling, fine lines and the like, and a preparation process and an application thereof, belonging to the technical field of cosmetics. Background Art

[0002] The eyes are the first part of our skin to show wrinkles, sagging and aging. The eye skin has the following characteristics: 1. The eye skin is the thinnest part of the skin, which is 1 / 3 to 1 / 5 of the thickness of the facial skin and is more fragile; 2. The eye skin is constantly stretched when blinking every day, which makes it more prone to wrinkles; 3. The skin around the eyes contains almost no sebaceous glands and sweat glands, resulting in the lack of a layer of sebum membrane around our eyes. Therefore, our eyes also lack a natural protective film, so the skin around the eyes is relatively drier and lacks moisture; 4. The eye skin has less fat content and lacks elastic fibers and collagen structure, so the eye skin is prone to lose elasticity and even premature aging; 5. The nerve fibers and capillaries around the eyes are densely distributed, microcirculation is slow, the lymphatic system is inactive, melanin deposition is obvious, and poor blood circulation leads to dull eyes and fatigue.

[0003] The condition of the eyes is the easiest to reveal age and make us look old. Skin problems often appear first in the eyes. Irregular lifestyle and improper use of eyes can easily cause dull and sagging eye skin, which can lead to edema, dark circles, eye bags, crow's feet and other problems. Eye care has gradually become a part of consumers' attention. More and more related products have entered the market, but most of them have a single mechanism of action and cannot fundamentally solve many eye problems. Strong products may even cause irritation or lead to the formation of fat particles.

[0004] The formation of dark circles is mainly related to pigmentation and vascular changes. According to the formation mechanism of dark circles, they can be divided into four types: vascular type, pigment type, relaxation type, and complex type. Vascular dark circles are often blue-black. Lack of sleep, eye fatigue and other factors cause the blood flow in the microvessels to slow down, the blood volume increases and the oxygen consumption increases. The increase of hypoxic hemoglobin causes congestion and edema of the skin around the eyes, and dark circles appear after light reflection. Pigmented dark circles are tea-black, mainly due to long-term sun exposure, excessive dry skin, and blood retention causing pigment deposition around the eyes. Relaxed dark circles are caused by aging and thinning of the eye skin and loss of collagen, resulting in depressions, showing blue shadows at the bottom of the eye socket. Complex dark circles are a combination of multiple factors. Therefore, the main ways to remove dark circles are to promote blood circulation, thicken the skin around the eyes, and dilute pigments.

[0005] Eye bags are generally believed to be caused by excessive orbital fat accumulation, degenerative relaxation of the lower eyelid tissue, and low tension. Due to excessive orbital fat accumulation or weak lower eyelid support structure, the original balance is changed. Under the action of gravity and gravity, the orbital fat under the eyeball slides down and bulges, gradually forming eye bags. As the eye skin gradually ages, the elasticity of the tissue around the eye socket decreases, causing the fat bag in the lower eyelid that protects the eyeball to slowly bulge out of the eye socket, forming eye bags.

[0006] Eye swelling is usually caused by staying up late, emotional stress, etc., which causes fatigue, slow lymphatic circulation, insufficient blood supply, and increased capillary permeability, causing a large amount of protein to leak into the tissue fluid, reducing the osmotic pressure of the fluid in the blood vessels and increasing the colloid osmotic pressure of the tissue fluid, causing eye swelling. Fine lines around the eyes are mostly caused by rich facial expressions, reduced moisture content, and reduced collagen content. Therefore, the skin around the eyes is sensitive and fragile, and requires daily targeted special care.

[0007] Cambodian garcinia cambogia extract is often used in eye care products. Its main active ingredient, hydroxycitric acid, can effectively improve problems such as fat eye bags and dark circles. Horse chestnut extract is also commonly used in eye care products for its excellent efficacy. It can promote microcirculation, eliminate eye edema, and effectively reduce the formation of pseudo eye bags. Cambodian garcinia cambogia extract with a high content of hydroxycitric acid usually has problems such as poor stability and easy crystallization in cosmetic applications. Horse chestnut extract with a high content of aescin also has the problem of poor water solubility and difficulty in application. Low-content Cambodian garcinia cambogia extract and horse chestnut extract have the problem of poor efficacy. Summary of the invention

[0008] In order to solve the shortcomings of the above-mentioned technologies, the present invention provides a composition for reducing skin problems around the eyes, a preparation process and an application thereof. Weakly alkaline amino acids such as arginine are used to improve the stability and utilization rate of hydroxycitric acid in Cambodian garcinia fruit extract, and lactobionic acid is used to improve the water solubility of aescin in the solution, making the entire system more stable. The pH value of the entire composition system reaches 5-7, which is close to the pH value of the skin itself while ensuring their efficacy well, and finally a composition that can comprehensively solve eye problems is obtained.

[0009] In order to solve the above technical problems, the technical solution adopted by the present invention is: a composition for reducing skin problems around the eyes, the composition comprising the following raw materials in parts by weight: Cambodian Garcinia cambogia extract: 5-15 parts, horse chestnut extract: 10-20 parts, lactobionic acid: 5-15 parts, weakly alkaline amino acids: 0.5-5 parts, and water is added to make up to 100 parts.

[0010] The composition comprises the following raw materials in parts by weight: 10 parts of Cambodian garcinia cambogia extract, 15 parts of horse chestnut extract, 10 parts of lactobionic acid, and 5 parts of weakly alkaline amino acids, which are supplemented with water to 100 parts;

[0011] The amino acid is any one of arginine, histidine, lysine, and aspartic acid.

[0012] A preparation process of a composition for reducing skin problems around the eyes, the preparation process is as follows: weigh each raw material according to the above weight portion, first mix and stir the horse chestnut extract, lactobionic acid, and water to dissolve, heat to 40°C-50°C, and after complete dissolution, add Cambodian garcinia cambogia extract and weakly alkaline amino acids to the above solution for mixing, ultrasonically stir until complete dissolution, and then filter with a 200-mesh filter cloth to obtain a transparent yellow solution.

[0013] Cambodian Garcinia Cambogia Extract can be an extract based on water, alcohol, water-alcohol or organic solvent, and its main active ingredient is hydroxycitric acid. Hydroxycitric acid is a strong competitive inhibitor of ATP citrate lyase, which can reduce acetyl-CoA in the cytoplasm by inhibiting ATP citrate lyase, and ultimately reduce fat synthesis and accumulation, and to a certain extent improve the problem of eye bags caused by fat accumulation; hydroxycitric acid can also inhibit the signal factors in the melanin synthesis pathway from the source, thereby inhibiting the formation of melanin, fundamentally blocking melanin, reducing pigment deposition, and effectively removing pigmented dark circles.

[0014] Aescin, the main active ingredient in horse chestnut extract, is a mixture of triterpenoid pentacyclic acylated saponins extracted from the seeds of horse chestnut. Horse chestnut extracts containing high concentrations of aescin have low water solubility and will produce flocculents when dissolved, which affects their application. Aescin can resist tissue edema, reduce vascular permeability, prevent water accumulation in tissues, and quickly eliminate the heavy feeling and pressure caused by local edema. It can effectively improve the integrity of blood vessel walls by filling tiny gaps and tiny visible gaps in small veins and capillaries, relieve varicose veins by promoting water exchange in capillaries, promote microcirculation, eliminate eye edema, and effectively reduce the formation of pseudo-eye bags.

[0015] Lactobionic acid is a compound formed by grafting one molecule of galactose and one molecule of gluconic acid. Lactobionic acid can cooperate with hydroxycitric acid in Cambodian Garcinia Cambogia Extract to dissolve protein conjugates in the stratum corneum, accelerate the shedding of aging keratinocytes, promote cell renewal, brighten the skin around the eyes, and reduce inflammation, avoiding the formation of dark circles caused by inflammation. Lactobionic acid can also cooperate with aescin to moisturize: aescin can inhibit the activity of hyaluronidase, protect hyaluronic acid from degradation, and promote the maintenance of moisture in the skin around the eyes; lactobionic acid can also absorb moisture in the air, increase the moisture content of the skin, and form a protective film to prevent moisture loss and protect the skin from damage by the external environment. In addition, lactobionic acid can also make the keratinized plugs around the pores fall off easily, unclog the hair follicle duct, prevent pore blockage, and effectively reduce the risk of fat particles while caring for the skin around the eyes.

[0016] Regarding eye aging, Cambodian Garcinia Cambogia Extract, Horse Chestnut Extract and Lactobionic Acid all have powerful antioxidant effects, which can synergistically neutralize free radicals, reduce cell oxidative damage, stimulate collagen synthesis in the skin, increase skin elasticity, improve the elasticity and firmness of the eye skin, reduce the appearance of wrinkles and fine lines, and help delay eye skin aging.

[0017] Arginine is one of the essential amino acids for the human body. It can relax the blood vessel wall and promote blood circulation in the eyes. It is weakly alkaline. Hydroxycitric acid is an organic acid containing 2 hydroxyl groups and 3 free carboxyl groups. It is a weak acid. It can form salts under the alkaline conditions provided by arginine, which effectively protects the biological activity of hydroxycitric acid as the main active substance of Cambodian Garcinia Cambogia extract and improves its utilization rate. At the same time, in the process of separating and extracting Cambodian Garcinia Cambogia, hydroxycitric acid is very easy to condense into lactone, which also leads to its reduced biological activity. The addition of arginine hydrolyzes the hydroxycitric acid lactone in part of the Cambodian Garcinia Cambogia extract under alkaline conditions, and the lactone ring opens and then becomes hydroxycitrate, which overcomes the poor stability of hydroxycitric acid in the matrix and the shortcomings of easy crystallization and precipitation, and further improves the stability and utilization rate of hydroxycitric acid in the Cambodian Garcinia Cambogia extract. At the same time, the addition of lactobionic acid helps to improve the water solubility of aescin in the solution, making the whole system more stable, and the pH value of the whole composition system reaches 5-7, which is close to the pH value of the skin itself.

[0018] In summary, the present invention starts from the formation mechanism of dark circles, eye bags, edema and eye fine lines, and synergizes Cambodian garcinia extract, horse chestnut extract, lactobionic acid and arginine to inhibit eye fat accumulation, improve vascular microcirculation, promote cell renewal, inhibit melanin, etc., thereby effectively solving various dark circles, eye bags, eye edema, eye fine lines and other problems. The addition of lactobionic acid and arginine further improves the stability of aescin and hydroxycitric acid in the system. The composition is mild and non-irritating, while avoiding the risk of fat granule formation, and is suitable for a variety of eye problems and comprehensively protects the eye skin.

[0019] The invention discloses a composition for reducing skin problems around the eyes, which is applied to medicines or eye care products. The composition of the invention is used in medicines and cosmetics (eye care products), so that the medicines and cosmetics have the effects of improving and reducing aesthetic problems of the eyes, such as dark circles, eye bags, edema and eye wrinkles.

[0020] The eye care product also contains auxiliary materials, which are at least one of preservatives, chelating agents, flavors, oils, moisturizers, thickeners, surfactants, emulsifiers, colorants, antioxidants, penetration aids, and skin feel regulators.

[0021] Excipients refer to conventional excipients currently used in cosmetics. Those skilled in the art can select appropriate amounts of excipients based on existing technologies and the dosage form and purpose of the cosmetics, as long as the selected excipients do not hinder the desired functions of the composition of the present invention. Cosmetic excipients can be found in the "Catalogue of Used Cosmetic Raw Materials".

[0022] The eye care product further comprises a preservative, which is at least one of formaldehyde releasers, parabens, halogens, quaternary ammonium salts, organic acids, and alcohols. Preferably, the preservative is an organic acid preservative or an alcohol preservative;

[0023] The eye care product also contains a moisturizer, which is at least one of glycerin, propylene glycol, butylene glycol, 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate, sugars, and polyethylene glycol.

[0024] The eye care product also contains a thickener, which is at least one of cellulose, xanthan gum, carrageenan, gellan gum, pectin, locust bean gum, carbomer, polyacrylic acid copolymer, magnesium aluminum silicate, lithium magnesium silicate, and sodium magnesium silicate.

[0025] The eye care product also contains an antioxidant, which is at least one of tocopherol, tocopherol acetate and butylated hydroxytoluene.

[0026] The eye care product also contains a penetration enhancer, which is at least one of laurocapram, ethoxydiglycol, polyglycerol-10, eicosapentaenoic acid ester, tetradecanoic acid ester and glycerol.

[0027] The preservative, moisturizer, thickener, antioxidant and penetration aid of the eye care product include but are not limited to the above-mentioned preferred substances.

[0028] Preferably, the eye care product is a leave-on product in the form of a liquid, gel, emulsion or cream. From the perspective of performance, the eye care product is more preferably in the form of a cream when used as a cosmetic. The preparation method of the eye care product can be selected by a person skilled in the art according to conventional methods.

[0029] The present invention aims at the problems that are prone to occur in the eye area, takes into account the formation mechanism of dark circles, eye bags, eye swelling and eye fine lines, and starts from the inhibition of fat masses and the promotion of capillary microcirculation and cell renewal. A composition for reducing the problems of dark circles, eye bags, eye swelling, fine lines and the like in the skin around the eyes is invented. When applied to eye care products, it has multiple effects such as reducing dark circles, effectively removing eye bags, and tightening and refreshing the skin around the eyes. It is gentle and non-irritating, and at the same time effectively prevents the formation of fat particles, and comprehensively protects the skin around the eyes. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a visual comparison chart of the eye condition after using the eye care product prepared in Example 1 for 28 days.

[0031] Figure 2 This is a comparison chart of fine eye lines before and after using the eye care product prepared in Example 1 of the present invention.

[0032] Figure 3 This is a comparison chart of pigmentation before and after use of the eye care product prepared in Example 1 of the present invention. DETAILED DESCRIPTION

[0033] The present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0034] A composition for reducing skin problems around the eyes, comprising the following components in parts by weight: A. Cambodian garcinia cambogia extract; B. horse chestnut extract; C. lactobionic acid; D. weakly basic amino acids (the amino acids include but are not limited to arginine, histidine, lysine, aspartic acid, etc., for pH adjustment, preferably, arginine is a commonly used pH adjustment component).

[0035]

[0036] Example 1: This example provides a composition for reducing problems around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., which is composed of the following raw materials in parts by weight: 10 parts by weight of Cambodian Garcinia cambogia extract, 15 parts by weight of Aesculus hippocastanum extract, 10 parts by weight of lactobionic acid, and 5 parts by weight of arginine, and water is added to make up to 100 parts.

[0037] Preparation method:

[0038] Weigh the raw materials according to the above weight portions, first mix and stir the horse chestnut extract, lactobionic acid and water to dissolve, and heat them to 40-50°C appropriately. After complete dissolution, add Cambodian garcinia cambogia extract and arginine and mix, ultrasonically stir until complete dissolution, and then filter with a 200-mesh filter cloth to obtain a transparent yellow solution.

[0039] Example 2: This example provides a composition for reducing problems around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., which is composed of the following raw materials in parts by weight: 5 parts by weight of Cambodian Garcinia cambogia extract, 10 parts by weight of Aesculus hippocastanum extract, 5 parts by weight of lactobionic acid, 0.5 parts by weight of aspartic acid, and water is added to make up to 100 parts.

[0040] Example 3: This example provides a composition for reducing problems around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., which is composed of the following raw materials in parts by weight: 15 parts by weight of Cambodian Garcinia cambogia extract, 20 parts by weight of Aesculus hippocastanum extract, 15 parts by weight of lactobionic acid, and 5 parts by weight of arginine, supplemented with water to 100 parts.

[0041] Example 4: This example provides a composition for reducing problems around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., which is composed of the following raw materials in parts by weight: 7 parts by weight of Cambodian Garcinia cambogia extract, 12 parts by weight of Aesculus hippocastanum extract, 8 parts by weight of lactobionic acid, and 2 parts by weight of aspartic acid, and water is added to make up to 100 parts.

[0042] Example 5: This example provides a composition for reducing problems around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., which is composed of the following raw materials in parts by weight: 13 parts by weight of Cambodian Garcinia cambogia extract, 18 parts by weight of Aesculus hippocastanum extract, 12 parts by weight of lactobionic acid, and 3 parts by weight of arginine, and water is added to make up to 100 parts.

[0043] Comparative Example 1: This embodiment provides a composition for reducing problems of the skin around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., composed of the following raw materials in parts by weight: 10 parts by weight of Cambodian Garcinia cambogia extract, 15 parts by weight of Aesculus hippocastanum extract, 1 part by weight of lactobionic acid, 0 parts by weight of arginine, and supplemented with water to 100 parts.

[0044] Comparative Example 2: This embodiment provides a composition for reducing problems of the skin around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., composed of the following raw materials in parts by weight: 20 parts by weight of Cambodian Garcinia cambogia extract, 15 parts by weight of Aesculus hippocastanum extract, 10 parts by weight of lactobionic acid, 0.5 parts by weight of arginine, and water is added to make up to 100 parts.

[0045] Comparative Example 3: This embodiment provides a composition for reducing problems of the skin around the eyes, such as dark circles, eye bags, eye swelling, fine lines, etc., composed of the following raw materials in parts by weight: 10 parts by weight of Cambodian Garcinia cambogia extract, 30 parts by weight of Aesculus hippocastanum extract, 5 parts by weight of lactobionic acid, and 5 parts by weight of arginine, supplemented with water to 100 parts.

[0046] The performance of the above-prepared composition is demonstrated below from the aspects of composition stability, hydroxycitric acid utilization rate and efficacy.

[0047] 1. Stability comparison:

[0048] The addition of arginine and lactobionic acid effectively improved the solubility and stability of hydroxycitric acid and aescin, respectively.

[0049]

[0050] As can be seen from the above table, the compositions of Examples 1-5 are all in a light yellow, transparent and stable state at different temperatures. Compared with Example 1, Comparative Example 1 has the same content of Cambodian Garcinia Cambogia extract and Aesculus hippocastanum extract, the portion of lactobionic acid is reduced to 1 part by weight, and the portion of arginine is 0 part by weight. In Comparative Example 1, the composition is obviously incompletely dissolved and precipitates, indicating that the addition of arginine and lactobionic acid improves the stability of aescin and hydroxycitric acid in the system.

[0051] Comparative Example 2 compared with Example 1: the added contents of horse chestnut extract and lactobionic acid were the same, the content of Cambodian garcinia cambogia extract components was increased to 20 parts by weight, and the arginine content was reduced to 0.5 parts by weight. Compared with Example 1, precipitation occurred in Comparative Example 2. This indicates that excessive Cambodian garcinia cambogia extract will affect the stability of the system, and adding an appropriate proportion of arginine helps to solve the application stability of hydroxycitric acid in Cambodian garcinia cambogia.

[0052] Comparative Example 3 and Example 1: The added contents of Cambodian Garcinia Cambogia extract and arginine are the same, the content of the horse chestnut extract component is increased to 30 parts by weight, and the lactobionic acid content is reduced to 5 parts by weight. Compared with Example 1, the composition in Comparative Example 3 is obviously incompletely dissolved and precipitated, indicating that the water solubility of the horse chestnut extract is low when it is excessive, and the addition of a corresponding proportion of lactobionic acid helps to improve the water solubility of aescin in the solution, making the entire system more stable.

[0053] In summary, excessive Cambodian garcinia cambogia extract will affect the stability of the system, and excessive horse chestnut extract will cause flocs to precipitate. Adding appropriate proportions of lactobionic acid and arginine can respectively improve the stability of aescin and hydroxycitric acid in the system. It can be seen that the addition amount of Cambodian garcinia cambogia extract and horse chestnut extract needs to be controlled, and synergistically enhanced with lactobionic acid and arginine to keep the whole system in a stable state.

[0054] 2. Comparison of the effect of arginine addition on hydroxycitric acid utilization:

[0055] Detection method: Refer to GB / T 33412-2016 Determination of hydroxycitric acid in biological products by high performance liquid chromatography. The test results are as follows:

[0056]

[0057]

[0058] Comparison of Example 1 and Example 1: The contents of Cambodian Garcinia Cambogia extract and Aesculus hippocastanum extract are the same, the portion of lactobionic acid is reduced to 1 weight portion, and the portion of arginine is 0 weight portion. The content of hydroxycitric acid in Comparative Example 1 is 0.8%, which is significantly lower than that in Example 1, indicating that the addition of arginine helps to improve the utilization rate of hydroxycitric acid.

[0059] Comparative Example 2 is compared with Example 1: the added contents of horse chestnut extract and lactobionic acid are the same, the content of Cambodian garcinia cambogia extract is increased to 20 parts by weight, and the content of arginine is reduced to 0.5 parts by weight. Although the content of Cambodian garcinia cambogia extract in Comparative Example 2 is increased to twice that of Example 1, the content of hydroxycitric acid is 3.6%, which is significantly lower than that in Example 1, indicating that adding an appropriate proportion of arginine helps to improve the utilization rate of hydroxycitric acid.

[0060] Comparative Example 3 and Example 1: The added contents of Cambodian Garcinia Cambogia extract and arginine are the same, the content of the horse chestnut extract component is increased to 30 parts by weight, and the content of lactobionic acid is reduced to 5 parts by weight. The content of hydroxycitric acid in Comparative Example 3 is 2.7%, which is lower than that in Example 1. It may be because the instability of the entire system affects the utilization rate of hydroxycitric acid.

[0061] In summary, the addition of arginine can effectively protect the biological activity of hydroxycitric acid as the main active ingredient in Cambodian Garcinia cambogia extract, and further improve the utilization rate of hydroxycitric acid.

[0062] 3. Efficacy comparison

[0063] (1) Inhibit the synthesis of triglycerides in adipocytes

[0064] Principle: Mouse 3T3-L1 adipocytes are cultured for a long time under specific conditions, and a large amount of triglycerides (TRGL) are synthesized and accumulated from the energy sources of glucose and fetal bovine serum provided by the culture medium. The accumulated TRGLs are stored in vesicles (liposomes) that can become very large, and then 0.15% of the composition in Examples 1-5 and Comparative Examples 1-3 is added according to the results of the cytotoxicity MTT experiment. After 10 days of co-culture, they are stained with oil red, which can penetrate into the fat vesicles contained in the cells and turn them red. At this stage, microscopic morphological analysis is performed by acquiring digital images. Finally, isopropanol is added to extract the colored TRGL in the adipocytes, and the red intensity of the cell lysate is proportional to the amount of triglycerides in the cells. The triglyceride content is detected by the absorbance of the microplate reader at 570±30nm to measure the ability of the combination to improve eye bags. The results are shown in the following table:

[0065] sample Triglyceride content (%) Negative control 100 Positive Control 53.4 Example 1 41.0 Example 2 58.5 Example 3 36.9 Example 4 43.2 Example 5 39.3 Comparative Example 1 73.9 Comparative Example 2 60.4 Comparative Example 3 59.1

[0066] Comparative Example 1 compared with Example 1: the contents of Cambodian Garcinia Cambogia extract and Horse Chestnut extract are the same, the portion of lactobionic acid is reduced to 1 weight portion, and the portion of arginine is 0 weight portion. The content of triglyceride in Comparative Example 1 is 73.9%, which is much higher than the content of triglyceride in Example 1. Since the content of hydroxycitric acid in Comparative Example 1 is lower than that in Example 1, and hydroxycitric acid can reduce acetyl-CoA in the cytoplasm by inhibiting ATP citrate lyase, it ultimately plays a role in reducing fat synthesis and accumulation, and improves the eye bag problem caused by fat accumulation to a certain extent. It can be seen that the addition of Cambodian Garcinia Cambogia extract can effectively reduce the content of triglycerides, thereby further improving the eye bag problem.

[0067] Comparative Example 2 is compared with Example 1: the added contents of horse chestnut extract and lactobionic acid are the same, the content of the Cambodian Garcinia cambogia extract component is increased to 20 parts by weight, and the arginine content is reduced to 0.5 parts by weight. The content of triglyceride in Comparative Example 2 is 60.4%, which is significantly higher than that in Example 1. It can be seen that the stability and utilization rate of hydroxycitric acid in the Cambodian Garcinia cambogia extract can be improved by adding arginine, thereby enhancing the inhibitory effect of hydroxycitric acid on fat synthesis.

[0068] Comparative Example 3 compared with Example 1: the added contents of Cambodian Garcinia Cambogia extract and arginine were the same, the content of the horse chestnut extract component was increased to 30 parts by weight, and the lactobionic acid content was reduced to 5 parts by weight. The triglyceride content in Comparative Example 3 was 59.1%, which was higher than the triglyceride content in Example 1. It can be seen that the horse chestnut extract containing a high concentration of aescin in Comparative Example 3 had a low water solubility, resulting in an unstable system, thereby affecting the utilization rate of hydroxycitric acid, resulting in an increase in the content of triglycerides, further proving that the addition of Cambodian Garcinia Cambogia extract can effectively reduce fat synthesis and accumulation.

[0069] In summary, compared with Example 1-5, Comparative Example 1-3 has a higher content of triglycerides. Since the content of hydroxycitric acid in Comparative Example 1-3 is lower, the inhibition rate of fat synthesis is lower than that in Example 1-5, and its ability to improve fat-type eye bags is also correspondingly poorer. This further illustrates that the addition of arginine helps to increase the bioavailability of hydroxycitric acid in the Cambodian Garcinia cambogia extract, thereby reducing the content of triglycerides, reducing fat synthesis and accumulation, and thus improving fat-type eye bags.

[0070] In summary, through the synergistic effect of Cambodian Garcinia Cambogia Extract, Horse Chestnut Extract, lactobionic acid and arginine, the accumulation of fat around the eyes can be inhibited, thereby effectively solving problems such as eye bags and eye puffiness.

[0071] At the same time, the present invention also provides a medicine or eye care product containing the above composition for reducing skin problems around the eyes. The details are as follows:

[0072] Application Example: The composition of Example 1 having multiple functions of reducing dark circles and tightening eye skin is prepared into an eye care product, and the formula is shown in the following table:

[0073] The preparation method is as follows: accurately weigh phase A, heat in a water bath at 80°C, quickly homogenize for 5 minutes until uniform, and set aside; accurately weigh phase B, heat and dissolve phase B, add phase B to phase A, homogenize for 5 minutes, and stir; reduce the temperature of the material to 60°C, add phase C2, stir it evenly, beat it lightly for homogenization, cool it down, then add phase C1 and phase D, stir and cool it down, and discharge the material.

[0074]

[0075]

[0076] (1) Irritation Verification: Chicken Embryo Chorioallantoic Membrane Irritation Test

[0077] The chicken embryo chorioallantoic membrane test is to detect whether the product is irritating under different concentration ratios. The eggshell of the air chamber of a nine-day-old chicken embryo is peeled off to expose the white egg membrane, which is then moistened with 0.9% sodium chloride solution and the inner membrane is removed with tweezers to ensure that the vascular membrane is not damaged. After confirming that the damaged blood vessels are not damaged, preliminary experiments and formal experiments are carried out.

[0078] Experimental steps:

[0079] S1. Experimental groups, each group has at least 6 chicken embryos, each experiment needs to set up negative control, positive control and blank control;

[0080] S2, CAM (chorioallantoic membrane) preparation, 9-day-old chicken embryos were examined by egg candling, the air chamber eggshell was peeled off, physiological saline was dripped on the surface of the eggshell membrane to fully moisten it, and then the eggshell membrane was removed;

[0081] S3, coating the test substance on the surface of the plastic film and turning the film over so that the sample surface contacts the CAM;

[0082] S4. After 3 minutes of action, remove the film, immediately observe the degree of change of each toxic effect, and give a score (ES).

[0083] Observation results:

[0084] Bleeding: blood flows out of the blood vessels or capillaries of the CAM. No bleeding, mild bleeding, moderate bleeding, and severe bleeding are scored as 0, 1, 2, and 3 points, respectively.

[0085] Coagulation: refers to the denaturation of proteins inside and outside the blood vessels, manifested as thrombosis, closure swelling, the appearance of coagulation points inside and outside the blood vessels, milky turbidity outside the blood vessels, etc. No coagulation, mild coagulation, moderate coagulation, moderate coagulation are awarded 0, 1, 2, and 3 points respectively.

[0086] Vascular ablation: refers to the ablation of blood vessels on the CAM membrane. No ablation, mild ablation, moderate ablation, and severe ablation are scored as 0, 1, 2, and 3 points, respectively.

[0087] Result calculation and evaluation: Calculate the average ES score according to the following formula and determine the stimulus classification according to Table 1:

[0088] ES = (the sum of the extent of bleeding, coagulation, and vascular lysis observed in 6 chicken embryos) / 3

[0089] Table 1 ES results evaluation

[0090] End point score Irritation classification ES≤12 No / mild irritation 12<ES<16 Moderate irritation ES≥16 Severe irritation

[0091] Table 2 Chicken embryo irritation test results

[0092]

[0093] Experimental conclusion: This composition is non-irritating under the optimal concentration ratio conditions.

[0094] (2) Human trials to verify efficacy

[0095] Test Example 1: Eye Cream Safety Test

[0096] 30 healthy volunteers were selected, including women with wrinkles, dark circles or eye bags around the eyes and no history of skin allergies, aged between 27 and 60 years old, with an average age of 34±2 years old, and randomly divided into 2 groups, 15 people in each group, and the 2 groups were respectively smeared with the eye cream prepared in Example 1 and the blank matrix without Example 1. After cleansing the face in the morning and evening every day, evenly apply it to the area around the eyes until absorbed. The results show that after applying the eye cream prepared in Example 1, no allergic reactions such as redness of the eye skin were observed, and no fat particles were generated in the trial subjects, indicating that the eye care products prepared by the composition of the present invention are safe and have no adverse phenomena.

[0097] Test Example 2: Evaluation of the effects of eye cream on anti-wrinkle (increasing skin moisture content and wrinkle quantity), removing eye bags and dark circles

[0098] Before each test, the subjects were required to clean their eyes with clean water and dry them with a paper towel without fluorescent agent before the test. They were required to sit quietly for at least 30 minutes in a constant temperature and humidity environment (temperature 20-22°C, relative humidity 40%-60%). The fixed areas around the left and right eyes of the subjects were measured using the skin surface moisture loss tester Tewameter TM Hexagon (Courage & Khazaka, Germany) and the skin colorimeter (Courage & Khazaka, Germany). The changes in the stratum corneum moisture content and the skin ITA° value were recorded. Facial images were collected and eye bags were analyzed using Primos. The test results were collected in the following four time periods: before using the sample (D0), 14 days after using the sample (D14), and 28 days after using the sample (D28). The results are shown in the table below.

[0099]

[0100] Conclusion: The eye care product prepared in Example 1 has a significant effect of improving eye bags, dark circles and wrinkles compared with the blank matrix.

[0101] After using the sample for 28 days, the volume of eye bags decreased by 9.4%, ITA° increased by 6.27%, wrinkles decreased by 35.23%, and the moisture content of the stratum corneum increased by 19.25%. The volume of eye bags in the control group increased by 3.28%, ITA° decreased by 0.35%, wrinkles decreased by 5.22%, and the moisture content of the stratum corneum increased by 5.06%. Compared with the control group, the eye care product prepared in Example 1 has better effect. Eye bags are reduced, eye color becomes lighter, wrinkles are reduced, and the moisture content of the stratum corneum is increased.

[0102] VISIA intuitive comparison chart:

[0103] Before using the sample (D0): 28 days after using the sample (D28)

[0104] (1) Visual comparison of eye conditions

[0105] Subject 1 self-evaluation: Figure 1 As shown, after 28 days of use, the dark circles caused by obvious melanin deposition around the eyes were significantly improved, the melanin began to fade significantly, the fine lines around the eyes were also reduced, and the skin around the eyes became tighter.

[0106] (2) Comparison of eye fine lines before and after use: Subject 2: Figure 2 As shown, the feature count of periocular wrinkles decreased by 31% after 28 days of use.

[0107] (3) Comparison of pigmentation before and after use: Subject 3: Figure 3 As shown in the figure, it can be seen intuitively that after 28 days of use, the pigmentation at the beginning and end of the eyes has been significantly lightened.

[0108] The present invention reduces dark circles around the eyes, eye bags, edema, fine lines and other problems through the synergistic effect of aescin and hydroxycitric acid. Hydroxycitric acid can reduce acetyl coenzyme A in the cytoplasm by inhibiting ATP citrate lyase, and finally reduces fat synthesis and accumulation, thereby improving the eye bag problem caused by fat accumulation to a certain extent. At the same time, the use of lactobionic acid helps to increase the water-soluble arginine of aescin and protect the structural stability of hydroxycitric acid, making the whole system more stable.

[0109] The above implementation modes are not limitations of the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or substitutions made by technicians in this technical field within the scope of the technical solution of the present invention also belong to the protection scope of the present invention.

Claims

1. A composition for reducing skin problems around the eyes, characterized in that: The composition comprises the following raw materials in parts by weight: 5-15 parts of Cambodian garcinia cambogia extract, 10-20 parts of horse chestnut extract, 5-15 parts of lactobionic acid, and 0.5-5 parts of amino acids, which are supplemented with water to 100 parts, wherein the amino acids are arginine or aspartic acid.

2. The composition for reducing skin problems around the eyes according to claim 1, characterized in that: The composition comprises the following raw materials in parts by weight: 10 parts of Cambodian garcinia cambogia extract, 15 parts of horse chestnut extract, 10 parts of lactobionic acid, and 5 parts of amino acids, and water is added to make the raw materials 100 parts.

3. The preparation process of the composition for reducing skin problems around the eyes according to claim 1 or 2, characterized in that: The preparation process is as follows: weigh each raw material according to the above-mentioned mass parts, first mix and stir the horse chestnut extract, lactobionic acid, and water to dissolve, heat to 40°C-50°C, and after complete dissolution, add Cambodian garcinia cambogia extract and amino acids to mix, ultrasonically stir until complete dissolution, and then filter with a 200-mesh filter cloth to obtain a transparent yellow solution.

4. The use of the composition for reducing skin problems around the eyes according to claim 1 or 2, characterized in that: The composition is used for preparing eye care products.

5. The use of the composition for reducing skin problems around the eyes according to claim 4, characterized in that: The eye care product also contains auxiliary materials, which are at least one of preservatives, chelating agents, flavors, oils, moisturizers, thickeners, surfactants, emulsifiers, colorants, antioxidants, penetration aids, and skin feel regulators.

6. The use of the composition for reducing skin problems around the eyes according to claim 4, characterized in that: The eye care product also contains a preservative, which is at least one of formaldehyde releasers, parabens, halogen-containing preservatives, quaternary ammonium salts, organic acids, and alcohol preservatives.

7. The use of the composition for reducing skin problems around the eyes according to claim 4, characterized in that: The eye care product also contains a moisturizer, which is at least one of glycerin, propylene glycol, butylene glycol, 1,2-pentanediol, 1,2-hexanediol, sodium hyaluronate, sugars, and polyethylene glycol.

8. The use of the composition for reducing skin problems around the eyes according to claim 4, characterized in that: The eye care product also contains a thickener, which is at least one of cellulose, xanthan gum, carrageenan, gellan gum, pectin, locust bean gum, carbomer, polyacrylic acid copolymer, magnesium aluminum silicate, lithium magnesium silicate, and sodium magnesium silicate.

9. The use of the composition for reducing skin problems around the eyes according to claim 4, characterized in that: The eye care product also contains an antioxidant, which is at least one of tocopherol, tocopherol acetate and butylated hydroxytoluene.

10. The use of the composition for reducing skin problems around the eyes according to claim 4, characterized in that: The eye care product also contains a penetration enhancer, which is at least one of laurocapram, ethoxydiglycol, polyglycerol-10, eicosapentaenoic acid ester, tetradecanoic acid ester and glycerol.

Images