A seabuckthorn softwood cutting method based on two-stage method

Abstract

The invention provides a sea buckthorn softwood cutting method based on a two-stage method, comprising the following steps: S1: the incision of the cuttings in the small arch shed is healed, a humidification and heat preservation small arch shed is constructed in the greenhouse, and a mesh plastic basket is arranged in the small arch shed for arranging the cuttings; a gradient three-level warming and moisture-retaining measure is adopted to promote the healing management of the cuttings; S2: field cutting, soaking the cuttings in a nutrient preservative liquid before cutting: diluting "amino acid water-soluble fertilizer" by 1500 times with water, and then adding "effective ingredient" according to the dilution ratio of 1000 times. The content of 30% carbendazim and oxadiazine is 30%, so as to obtain a nutrient preservative mixture; the mesh plastic baskets together with the cuttings are taken out from the small arch shed one by one, and are put into the nutrient preservative mixture to soak for 10 minutes, and then are cut into the field one by one; flood irrigation after cutting in the field: after cutting, flood irrigation is carried out once before 8 am or after 18 pm every day, and each time of flooding irrigation, the cuttings are soaked in water for 4-6 hours, and flooding is continued for 7-10 days. After the white roots are formed at the lower incision of the cuttings, the number of days between flooding irrigation is gradually extended.

Description

Technical Field

[0001] The invention relates to a sea buckthorn softwood cutting method based on a two-stage method, and belongs to the technical field of sea buckthorn cultivation. Background Art

[0002] Applying genetic improvement theory to select and breed high-quality varieties and propagate through asexual cuttings is a necessary condition and foundation for cultivating high-quality sea buckthorn resources and developing the sea buckthorn industry. Softwood cuttings are the main way to solve the problem of sea buckthorn cuttings being difficult to root. However, Tibet's cold and dry climate cannot simultaneously meet the necessary temperature, humidity, and air permeability conditions for softwood cuttings, which restricts the cultivation of high-quality sea buckthorn resources in Tibet, and thus restricts the development of the Tibetan sea buckthorn industry.

[0003] The three elements of softwood cuttings, temperature, humidity, and air (air permeability), each have a strict numerical range. In other regions, when softwood cuttings are carried out in June, it is hot and rainy in summer, and the temperature and humidity conditions are easy to meet. However, in Tibet's high, cold, and dry climate conditions, the temperature and humidity conditions are difficult to meet. The existing softwood cutting technology and its operating procedures must be improved according to Tibet's natural conditions.

[0004] Current status of softwood cutting technology and problems to be overcome:

[0005] 1. Current status of temperature problem:

[0006] There are strict requirements on temperature before the cuttings heal. The full-light automatic spray cutting equipment is used for field sea buckthorn tender branch cuttings, and the tender branch cutting sea buckthorn seedlings are cultivated on a large scale. This is a feasible and mature large-scale seedling method in the mainland, but the high, cold and dry climate of Tibet does not have the temperature conditions for the healing of the cuttings required for tender branch cuttings. Taking Fuxin City, Liaoning Province as an example, in the tender branch cutting season in June, the average high temperature was 29℃ and the average low temperature was 20℃, which met the optimal temperature value of 18-30℃ for sea buckthorn tender branch cuttings; while taking Zangang County, Shannan City, Tibet as an example, in the tender branch cutting season in June, the average high temperature was 23℃ and the average low temperature was 7℃, and even frost occasionally occurred, which did not meet the temperature value of 18-30℃ required for tender branch cuttings.

[0007] If seedlings are grown in a greenhouse, taking the greenhouse experiment in Zhanang County, Shannan City, Tibet as an example, although the temperature is cooled by spraying under the strong sunlight during the day, the temperature still reaches the temperature required for rapid healing of cuttings. However, in the early morning, the temperature inside the greenhouse is affected by the low temperature of less than 7°C outside the greenhouse and drops below 18°C ​​to below 15°C. The effective accumulated temperature for plant growth is determined by the lowest temperature. If the lowest temperature is lower than the required value, the high temperature value is invalid. Therefore, even if the softwood cuttings are in the greenhouse, the temperature required for rapid healing of softwood cuttings cannot be met.

[0008] 2. The contradiction between humidity and air permeability:

[0009] Before the cuttings heal, it is necessary to ensure the air permeability of the incision under the branch and ensure that the cuttings have sufficient water. These are two factors that are difficult to take into account at the same time. If you want to ensure that the cuttings have sufficient water, you must control the relative humidity of the air at around 90% through continuous sprinkler irrigation. Continuous sprinkler irrigation causes the relative humidity of the cutting medium to exceed 60%, while the relative humidity of the cutting medium with the best air permeability should be between 20 and 25%. When the relative humidity of the cutting medium is higher than 60%, there is severe lack of oxygen. Because the air permeability requirements and humidity requirements are difficult to balance and control, the incision under the cuttings is generally lacking in oxygen and rots, the rooting time is prolonged, and the survival rate is low due to excessive humidity in the matrix, or a large number of cuttings dry up due to insufficient humidity and low survival rate.

[0010] In order to solve the contradiction between humidity and air permeability, the existing technology uses river sand with good air permeability as a substrate for softwood cuttings. This method still cannot meet the oxygen required for rapid healing of the incision under the cuttings. Lack of oxygen and bacterial infection in the incision inhibit the healing of the incision, resulting in a high rot rate of the cuttings. The rooting time after cuttings is as long as more than 1 month, and the survival rate is unstable, only between 30% and 70%.

[0011] 3. Current status of anti-corrosion effect

[0012] The cuttings are very susceptible to infection and rot before they heal. The existing softwood cutting technology is to insert the branches separated from the mother plant directly into the matrix. The lower incision of the cuttings is in contact with the matrix, which hinders the formation of an oxide layer on the incision. The bacteria in the matrix directly infect the cuttings, thereby increasing the chance of bacterial infection. Once infected and moldy, even if fungicides are sprayed or even irrigated, it is difficult to restore a good survival rate.

[0013] 4. Rooting status of cuttings after incision healing

[0014] There is a saying about root vitality: "white roots are strong, yellow roots are life-saving, and black roots are diseased." After long-term comparative experiments by the applicant, it was found that after the incision of the sea buckthorn cuttings healed, proper soaking in water would result in strong seedlings with white and thick roots, while using the existing softwood cutting technology with sprinkler irrigation would result in thin seedlings with yellow and thin roots.

[0015] 5. Current status of funding costs:

[0016] There are three major costs and financial inputs in the existing softwood cutting technology. The first is the cost of the cutting substrate, which requires the use of river sand that does not contain organic matter and has good air permeability and water retention, and the laying of a sand tray substrate with a sand layer thickness of about 10 cm to ensure the air permeability and water retention of the cuttings, which incurs the cost of buying and laying sand. The second is the purchase and operation cost of sprinkler irrigation facilities, which also includes the cost of setting up circuits. The third is the cost of pruning branches and leaves of cuttings. Softwood cuttings require only 3-5 branches and leaves at the top to be retained, and the rest are all pruned off, mainly to prevent the branches from falling leaves, affecting the air permeability of the substrate and causing the cuttings to rot.

[0017] In summary, the existing sea buckthorn softwood cutting technology has the problems of easy rot of cuttings, low survival rate, weak seedlings, and high cost investment. In particular, the temperature factor directly restricts the large-scale softwood cutting in Tibet. Summary of the invention

[0018] In order to solve the defect that the existing sea buckthorn softwood cutting technology is difficult to be successfully applied in the high-cold environment of Tibet due to the restriction of temperature factors, and to overcome the defects of the existing softwood cutting technology that the cuttings are easy to rot, the survival rate is low, the seedlings are weak, and the cost investment is large, the present invention provides a sea buckthorn softwood cutting method based on a two-stage method, which is characterized by comprising the following steps:

[0019] S1 small shed cuttings incision healing

[0020] S1.1 Build a small humidifying and heat-insulating arch shed in the greenhouse, and set up a mesh plastic basket in the small arch shed for placing cuttings;

[0021] S1.2 Use a gradient three-level temperature control measure to promote the healing of cuttings, as follows:

[0022] After soaking the cuttings in the rooting agent solution for 30 minutes, take out the cuttings and place them in a mesh plastic basket, with the lower cut of the cuttings facing downwards. The outer surface of the small arch shed is covered with a plastic shade net and agricultural shed film in turn, and management is carried out in three periods every day;

[0023] The first management is from 10:00 to 10:30 in the morning. When the temperature in the small shed rises to 20-30℃, turn on the micro-spraying equipment and wait until the humidity in the small shed is above 90% before turning it off. It takes about 30 minutes.

[0024] During the second management period from 12:00 to 12:30 noon, the temperature in the small shed rose to above 30℃, the plastic shade net and agricultural greenhouse film were uncovered, the micro-spraying equipment was turned on, and micro-spraying was continued until the humidity in the small shed was above 90% and the water depth on the ground was 15cm. The micro-spraying equipment was turned off, and the outer surface of the small shed was covered with a plastic shade net to keep it moist until 17:00 in the afternoon. During the entire second management period, although spraying water in the small shed caused a drop in temperature, the temperature outside the greenhouse was around 20℃, and under the action of high-intensity sunlight energy in Tibet, the temperature in the greenhouse rose to above 50℃, so the temperature in the small shed could still be maintained at 25-30℃, thus forming a gradient three-level warming.

[0025] During the third management, after 17:00 in the afternoon, the temperature inside the small shed dropped to around 20°C. Agricultural greenhouse film was added to the outside of the plastic shade net to increase the temperature, which brought the temperature inside the small shed back to above 30°C.

[0026] It should be noted that the agricultural greenhouse film must be covered tightly to keep warm, so that until the early morning of the next day, the temperature outside the greenhouse is generally below 7°C, the temperature inside the greenhouse is 10-15°C, and the temperature inside the small arch shed remains above 18°C ​​until the early morning, thus forming a gradient three-level insulation, which meets the 18-30°C temperature requirement for the healing of cuttings.

[0027] The small arch shed can not only increase temperature and keep warm, but also take into account humidity, air permeability and anti-corrosion. It fully meets the requirements of the cuttings separated from the mother plant for temperature, oxygen, humidity and the optimal anti-corrosion range during the healing process, and effectively overcomes the constraints of the cold and dry climate of the Tibetan Plateau on large-scale softwood cuttings and seedling cultivation.

[0028] The applicant has found through research that the gradient three-level temperature control measure solves the contradiction between humidification and heat preservation caused by sprinkler irrigation, and achieves a perfect combination of humidification and heat preservation. Since the base temperature in the plastic greenhouse is above 50°C from 12:00 to 16:00, despite the cooling caused by micro-spraying, the temperature in the small arch shed can be maintained at 25-30°C, which meets the optimal temperature range standard for promoting the healing of cuttings. After 4 hours of continuous sprinkler irrigation, the surface water in the small arch shed was 15cm deep, and the lower part of the cuttings was immersed in the accumulated water to absorb enough water to promote the healing of the cuttings. In addition, during this period, the covering sunshade nets and agricultural greenhouse films were uncovered, and the photosynthesis of the cuttings leaves exposed to sunlight promoted the formation of callus tissue and accelerated healing.

[0029] Through the combined measures of covering with plastic shade nets, micro-spraying, and soaking in water in the small shed, the cuttings not only do not lose water, but are also able to absorb enough water, so that the cuttings obtain sufficient water required for life activities, effectively promote callus formation and accelerate healing.

[0030] The cuttings are arranged sparsely in a mesh plastic frame, with gaps between the cuttings for ventilation; a 5-10 cm ventilation gap is left at the bottom of the plastic basket, so that the cuts under the cuttings can be in contact with the air for a long time to oxidize and form an oxide layer, blocking the infection of pathogens and preventing the cuts of the branches from rotting. Once a slight sign of rot is found through daily observation, the "30% active ingredient content of carbendazim and oxazolidinone" produced by Zhengzhou Zhengshi Chemical Products Co., Ltd. is immediately diluted 1000 times with water to prepare a sterilization solution, and the branches in the plastic basket are quickly soaked one by one, which is convenient, fast, thorough, and antiseptic, and the spread of rot is curbed in time. Good air permeability and antiseptic measures effectively promote the formation of callus tissue to accelerate healing.

[0031] The bottom of the grid plastic frame for placing cuttings is 5 to 10 cm from the ground, forming air convection in the small arch shed to enhance the ventilation of the cuttings. The air permeability and humidity between the cuttings are in excellent condition. There is no need to prune the branches and leaves of the cuttings. All leaves will not turn yellow and fall off within a few days to cause mildew, saving the labor cost of pruning the branches and leaves of the cuttings.

[0032] The combination of the above measures will cause the incision under the cutting to swell slightly after 3 to 5 days, indicating that the goal of promoting healing has been achieved. At this time, the branch has the vitality to resist corrosion and take root. Next, as long as the water required for life activities is sufficient, the callus tissue will begin to differentiate and gradually form a root system. The time is ripe to transfer the cuttings from the small greenhouse to the field.

[0033] Measures to promote the healing of cuttings are taken in the small shed for a total of 3 to 5 days, and then the cuttings are taken out for field cuttings. That is, each batch of cuttings uses the small shed for only 3 to 5 days, and the small shed can be reused efficiently. The grid plastic frame with a bottom area of ​​0.24㎡ for arranging cuttings can arrange 500 cuttings at a time. A 10㎡ small shed can arrange 25,000 cuttings. Assuming that 10,000 cuttings are planted per mu for field cuttings, it can be used for the cuttings required for 2.5 mu of field, forming an efficient flow operation mode from promoting the healing of cuttings in the small shed to field cuttings.

[0034] S2 field cuttings

[0035] S2.1 Soak the cuttings in nutrient preservative solution before transplanting: dilute the amino acid water-soluble fertilizer with water 1500 times, and then add "30% active ingredient content of carbendazim and oxadiazine" produced by Zhengzhou Zhengshi Chemical Products Co., Ltd. according to the dilution ratio of 1000 times to obtain a nutrient preservative mixed solution; take out the mesh plastic baskets and cuttings from the small arch shed one by one, and put them into the nutrient preservative mixed solution for 10 minutes, so as to supplement the cuttings with nutrients and preservatives, and to allow the cuttings to absorb enough water; after the soaking, transplant the cuttings into the field;

[0036] S2.2 Flood irrigation after field cuttings: After cuttings, flood irrigation should be carried out once every morning before 8 am or after 6 pm to avoid the temperature drop caused by flooding during the day. Each time of flooding, keep the water stagnant for 4 to 6 hours to soak the cuttings. Flooding should be continued for 7 to 10 days. White roots will form under the incision of the cuttings. Thereafter, the number of days between flooding can be gradually increased.

[0037] Tibet has abundant river water resources and high dissolved oxygen content. In addition, Tibet's soil is highly permeable. The shallow water accumulated after river flooding will all seep away within 3 hours. Through flooding every day, the ground for grafting branches is alternately soaked in water and the ground is dry and breathable, which will not cause waterlogging and hypoxia.

[0038] The method of using flooding measures to keep the cuttings sufficiently moisturized fully utilizes the characteristics of the Tibetan Plateau, which has abundant water resources, strong soil permeability, and fast water penetration, to achieve alternation between water immersion and surface waterlessness, thereby achieving a perfect combination of humidity and air permeability. It saves the cost of laying 10 cm thick water-permeable and highly breathable river sand as a cutting medium for softwood cuttings, saves the cost of purchasing and operating sprinkler irrigation facilities, and greatly reduces the cost of softwood cuttings.

[0039] Seabuckthorn originated from the Qinghai-Tibet Plateau. Wild seabuckthorn in Tibet is mainly distributed in rivers with long-term flowing water. There are thousand-year-old wild seabuckthorn forests in many rivers in Tibet, indicating that proper water accumulation is conducive to the differentiation and growth of seabuckthorn root system and enhances the vitality of seabuckthorn root system. Through three major soaking experiences, from micro-spraying water in small arch sheds to soaking in nutrient preservative solution to replenish water before field cuttings and regular flooding and soaking in water every day after field cuttings, the branches are not only not dehydrated, but also can get the effect of replenishing enough water. The cuttings obtain sufficient water required for life activities to accelerate the differentiation of callus tissue and accelerate the formation of roots.

[0040] Further, the construction process of the small arch shed is as follows:

[0041] Level the land, pile up fences around the leveled land, insert scaffolding on the fence to form a small arch shed, dig a soil trough on the sunny side of the small arch shed, place one side of the plastic shade net and agricultural greenhouse film in the soil trough, compact and fix it with soil, and set up micro-spray pipes in the small arch shed.

[0042] Furthermore, the bottom of the mesh plastic basket is supported by wooden strips 5 to 10 cm above the ground. The cuttings are sparsely arranged through the mesh plastic frame, and there are gaps between the cuttings for ventilation; a 5 to 10 cm ventilation gap is left at the bottom of the plastic basket, so that the lower cut of the cuttings can be in contact with the air for a long time to form an oxide layer, blocking the infection of pathogens and preventing the branches from rotting.

[0043] Furthermore, during the first administration, the micro-spraying time is 10 to 15 minutes.

[0044] Furthermore, during the second management, the micro-spraying time is 4 to 6 hours.

[0045] Furthermore, the dimensions of the grafting slots for field cuttings are 5 to 10 cm in depth and 3 to 5 cm in width, and the row spacing between the grafting slots is 20 to 30 cm.

[0046] Beneficial technical effects achieved by the present invention:

[0047] The present invention divides the entire seedling raising process into two stages, i.e., a two-stage seabuckthorn softwood cutting method, based on the physiological characteristics and physiological demand differences of different links of softwood cutting seedling raising, combined with the climate characteristics of high cold, dryness, and the strongest light energy in Tibet, as well as the characteristics of strong water permeability of cultivated land in Tibet. Through segmented and meticulous management, the technical key points of each stage of seabuckthorn softwood cutting can be accurately controlled, different management measures are adopted in different stages, and the key points and difficulties of softwood cutting operation are decomposed, so that the softwood cutting operation procedures are programmed and standardized, so that the various physiological needs of softwood cutting at different stages can be fully met, and low-cost large-scale field softwood cutting can be realized.

[0048] The two-stage method of seabuckthorn softwood cuttings has different technical points and effects in the two stages: the first stage is intensive and meticulous management to promote healing. A small arch shed is built in the greenhouse to fully meet the requirements of oxygen, humidity, temperature and antiseptic when the cuttings are healing. When the lower incision swells slightly, it indicates that the initial healing is completed, which lasts for 3 to 5 days; the second stage is field cuttings to promote rooting. Through flooding and maintaining water accumulation time, the root primordium formation and differentiation requirements for water are fully met. The cuttings after initial healing are moved to the field for cuttings, and flooding is carried out once a day, similar to water cuttings. When the lower incision of the cuttings generates white roots, it indicates that the rooting is completed, which lasts for 7 to 10 days.

[0049] The two-stage method highlights that the key to the success of softwood cuttings is the technical focus and difficulty of promoting the rapid healing of the cuttings incisions. By creating a controllable space to strictly control and adjust the oxygen, humidity, and temperature required for the rapid healing of the cuttings incisions, the optimal healing limit of only 3 to 5 days is achieved.

[0050] Through the two-stage method, a programmed sea buckthorn softwood cutting flow operation seedling raising method is realized, which creates the technical procedures for large-scale field softwood cutting seedling raising. It solves the problem that sea buckthorn softwood cutting is difficult to succeed even in greenhouses under the cold and dry climate conditions of the Tibetan Plateau. It lays a technical foundation for the selection and rapid propagation of good varieties for the development of the Tibetan sea buckthorn industry and the localization of Tibetan sea buckthorn varieties.

[0051] The sea buckthorn softwood cutting method of the present invention can achieve a sea buckthorn survival rate of more than 95%, reduce labor intensity, improve work efficiency, improve survival rate and enhance seedling rate. Compared with the existing sea buckthorn softwood cutting technology currently used in the mainland, it has the following outstanding advantages: ① the survival rate is more than 30% higher, and the rooting time of cuttings is 10 to 15 days in total, and the rooting time is saved by about 20 days in total, thereby saving about 20 days of high-precision management time before rooting; ② the number of roots is increased by more than 2 times, the root system is strong, and the foundation of sea buckthorn seedlings is laid; ③ the capital investment and operation cost of the sand tray matrix and full-light spray facilities that are necessary for sea buckthorn softwood cuttings are saved, creating feasibility for low-cost large-scale field softwood cuttings in Tibet. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 The effects of different cutting arrangement methods on the growth of cuttings of the present invention, wherein A is a growth photo of the cuttings of Example 1, B is a growth photo of the cuttings of Comparative Example 1, C is a growth photo of the cuttings of Comparative Example 2, and C is a growth photo of the cuttings of Comparative Example 3;

[0053] Figure 2The effects of different water retention methods on the growth of cuttings after field cuttings of the present invention, wherein E is a growth photo of the cuttings of Example 1, F is a growth photo of the cuttings of Example 2, G is a growth photo of the cuttings of Example 3, H is a growth photo of the cuttings of Comparative Example 4, I is a growth photo of the cuttings of Comparative Example 5, and J is a growth photo of the cuttings of Comparative Example 6. DETAILED DESCRIPTION

[0054] The present invention will be further described below in conjunction with specific examples. The following examples are only used to more clearly illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the present invention.

[0055] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0056] Example 1 A sea buckthorn softwood cutting method based on a two-stage method

[0057] The specific steps are as follows:

[0058] S1 small shed cuttings incision healing

[0059] S1.1 Build a small humidifying and heat-insulating arch shed in the greenhouse, and set up a mesh plastic basket in the small arch shed for placing cuttings;

[0060] The width of the small arch shed is 2 meters and the length is 5 meters. The required materials are: ① Plastic sunshade net, shading rate 30-50%, width about 3 meters. ② Agricultural shed film, width about 3 meters and length 7 meters. ③ 10 sheds, about 3 meters long, the best material is bamboo strips, which can also be replaced by bendable steel bars and plastic pipes.

[0061] The construction process of the small arch shed is as follows:

[0062] Level the land of 2×5㎡, use the surplus soil to pile up 15cm high fences around the leveled land to store water, insert a trough on the fence to form a small arch trough 5 meters long and 2 meters wide, dig a 10cm deep soil trough on the sunny side of the small arch trough, place one side of the plastic shade net and agricultural greenhouse film in the soil trough, compact and fix it with soil, and set up micro-spray pipes in the small arch shed.

[0063] Arrangement of cuttings: Use a 60cm×40cm×20cm mesh plastic frame to arrange the cuttings, with the lower cut of the cuttings sparsely arranged toward the bottom of the frame to ensure air permeability between the cuttings. Use wooden strips to support the bottom of the frame 5 to 10 cm from the ground. The supports around the frame can prevent the cuttings from falling over. Place 500 10 cm long cuttings in each frame.

[0064] S1.2 Use a gradient three-level warming and moisturizing measure to promote the healing of the cuttings, as follows:

[0065] Dilute the "Indole-Naphthylacetic Acid" produced by Sichuan Lanyue Technology Co., Ltd. 600 times as a rooting agent aqueous solution, soak the cuttings for 30 minutes, take out the cuttings and place them in a mesh plastic basket, place the cuttings vertically with the lower cut facing downwards, cover the outer surface of the small arch shed with a plastic sunshade net and agricultural shed film in turn, and manage them in three periods every day;

[0066] The first management is from 10:00 to 10:30 in the morning. When the temperature in the small shed rises to 20-30℃, preferably 25℃, turn on the micro-spraying equipment for 10-15min. Wait until the humidity in the small shed is above 90% and then turn it off. At this time, the temperature in the small shed drops to 18-20℃. Since the small shed is covered with plastic shade nets and agricultural greenhouse films, the moisture in the small shed is sealed, and the humidity of 90% in the small shed can be maintained until 12 noon.

[0067] The second management, from 12:00 to 12:30 noon, when the temperature in the small shed rises to above 30℃, uncover the plastic sunshade net and agricultural shed film, turn on the micro-spraying equipment, and continue micro-spraying for 4-6 hours, keep the humidity in the small shed above 90%, the temperature at 25-30℃, the ground water height of 15cm, turn off the micro-spraying equipment, and cover the outer surface of the small shed with a plastic sunshade net;

[0068] The third management, after 17:00 in the afternoon, the temperature in the small shed dropped to about 20℃, and agricultural shed film was added outside the plastic shade net to increase the temperature in the small shed to above 30℃;

[0069] S2 field cuttings

[0070] S2.1 Soak in nutrient preservative solution before cutting: dilute the amino acid water-soluble fertilizer produced by Shanghai Huiguang Environmental Technology Co., Ltd. with water 1500 times, and then Dilute 1000 times Add "30% active ingredient metalaxyl and oxadiazine" produced by Zhengzhou Zhengshi Chemical Products Co., Ltd. in a ratio of 1000 to obtain a nutrient preservative mixture; take out the mesh plastic baskets and cuttings from the small arch shed one by one, and put them into the nutrient preservative mixture for 10 minutes, so as to supplement the cuttings with nutrients and preservation and allow the cuttings to absorb enough water; after the soaking, plant the cuttings in the field;

[0071] S2.2 Flood irrigation after field cuttings: After cuttings, flood irrigation should be carried out once every day before 8 am or after 6 pm. Each time of flooding, the water should be kept for 5 hours to soak the cuttings. Flooding should be continued for 7-10 days until white roots form at the lower incision of the cuttings. Gradually extend the number of days between flooding irrigations.

[0072] The dimensions of the cutting slots for field cuttings are 5 to 10 cm in depth and 3 to 5 cm in width, and the row spacing between the cutting slots is 20 to 30 cm.

[0073] Experimental Example 1 Effect of cutting arrangement on lower incision healing

[0074] The difference between Comparative Example 1 and Example 1 lies in the arrangement of the cuttings: narrow grooves with a depth of 10 cm are opened in sequence in the small arch shed, the cuttings are arranged in the grooves, and then covered with soil for about 5 cm.

[0075] The difference between Comparative Example 2 and Example 1 lies in the arrangement of the cuttings: the cuttings are arranged on the ground in the small shed, and the lower cut of the cuttings touches the ground.

[0076] The difference between Comparative Example 3 and Example 1 lies in the arrangement of the cuttings: the cuttings are arranged in a mesh plastic basket in the small shed, but the bottom of the frame is supported by wooden strips 20 cm from the ground.

[0077] Table 1 Statistics on the effect of different cutting placement methods on promoting healing

[0078]

[0079] It can be seen from Table 1 above that the optimal distance between the lower incision of the cuttings and the ground is 10 cm. Under the same temperature and water spraying management, the distance between the lower incision of the cuttings and the ground directly affects the healing effect. The lower incisions of the cuttings in Examples 1 and 2 are in contact with the soil, which hinders the formation of an oxide layer on the incision. Pathogens in the soil directly infect the cuttings, thereby increasing the chance of bacterial infection. In addition, the soil temperature is much lower than the temperature in the small shed, which delays the healing time. The lower incision of the cuttings in Example 3 is 20 cm from the ground, which increases the space between the incision and the ground, resulting in a loss of water soaking effect for the cuttings. The cuttings cannot obtain sufficient water and affect healing. Plant growth elements are related to each other. The lower incision of the cuttings in the embodiment has the best healing effect when it is 5 to 10 cm away from the ground, and other aspects are also strong, which will directly improve the survival rate after transplanting. Figure 1 It can also be seen that the leaves of the cuttings of Example 1 are green, while the cuttings of Comparative Examples 1-3 are all rotten or yellowed to varying degrees.

[0080] Experimental Example 2 Comparison of root-promoting effects of different water-retention methods after field cuttings

[0081] The difference between Example 2 and Example 1 is the flooding time: the flooding time is 4 hours.

[0082] The difference between Example 3 and Example 1 is the flooding time: the flooding time is 6 hours.

[0083] The difference between Comparative Example 4 and Example 1 is the method of retaining water after field cutting: daytime sprinkler irrigation is adopted, with sprinkler irrigation for 30 minutes every 20 minutes.

[0084] The difference between Comparative Example 5 and Example 1 lies in the water conservation method after field cuttings: the cuttings are soaked in water for about 1 hour after each flooding.

[0085] The difference between Comparative Example 6 and Example 1 lies in the water conservation method after field cuttings: the cuttings are soaked in water for about 8 hours after each flooding.

[0086] Table 2 Root-promoting effects of different water-retention methods after field cuttings

[0087] Different water conservation methods Rooting days Number of roots Survival rate Leaf condition on the 20th day after cutting Example 1 7 6 95％ Old green leaves Example 2 15 3 80％ Old leaves slightly green Example 3 7 6 95％ Old green leaves Comparative Example 4 40 2 30％ Old leaves wither Comparative Example 5 40 2 30％ Old leaves wither Comparative Example 6 7 6 95％ Old green leaves

[0088] From the above table 2 and Figure 2 It can be seen that sufficient water soaking time is directly related to the root promotion effect. The soaking of the cuttings in water for about 5 hours after each flooding in Example 1 is the same as the soaking of the cuttings in water for about 8 hours after each flooding in Example 6 and the soaking of the cuttings in water for about 6 hours after each flooding in Example 3. They can maintain the vigorous vitality of the old leaves for photosynthesis, promote the rooting speed, and promote the differentiation of callus to form multiple roots. Therefore, the 5-hour soaking time is sufficient to meet the root promotion needs. The daytime sprinkler irrigation in Comparative Example 4 and the soaking of the cuttings in water for about 1 hour after each flooding in Comparative Example 5 are not enough to meet the water required for the life activities of the cuttings, and are insufficient to maintain the water required for the vigorous vitality of the old leaves, causing the old leaves to gradually lose their photosynthetic ability until they wither, delaying the differentiation of callus.

[0089] The present invention has been disclosed above with preferred embodiments, but they are not intended to limit the present invention. Any technical solutions obtained by adopting equivalent replacement or equivalent transformation solutions fall within the protection scope of the present invention.

Claims

1. A seabuckthorn softwood cutting method based on a two-stage method, characterized in that The following steps are involved: S1 small shed cuttings incision healing S1.1 A small arch shed with adjustable temperature and humidity is constructed in the greenhouse, and a mesh plastic basket is set in the small arch shed for placing cuttings; the bottom of the mesh plastic basket is supported by wooden strips and is 5 to 10 cm above the ground; S1.2 Use a gradient three-level temperature control measure to promote the healing of cuttings, as follows: After soaking the cuttings in the rooting agent solution for 30 minutes, take out the cuttings and place them in a mesh plastic basket, with the lower cut of the cuttings facing downwards. The outer surface of the small arch shed is covered with a plastic shade net and agricultural shed film in turn, and management is carried out in three periods every day; The first management is from 10:00 to 10:30 in the morning. When the temperature in the small shed rises to 20-30℃, turn on the micro-spraying equipment and turn it off when the humidity in the small shed is above 90%. The second management, from 12:00 to 12:30 noon, when the temperature in the small shed rises to above 30℃, uncover the plastic sunshade net and agricultural shed film, turn on the micro-spraying equipment for continuous micro-spraying until the humidity in the small shed is above 90% and the ground water height is 15cm, turn off the micro-spraying equipment, and cover the outer surface of the small shed with a plastic sunshade net to keep it moist; The third management, after 17:00 in the afternoon, the temperature in the small arch shed dropped below 20℃, and agricultural shed film was added outside the plastic shade net to increase the temperature in the small arch shed to above 30℃; S2 field cuttings S2.1 Soak in nutrient preservative solution before cutting: Dilute the amino acid water-soluble fertilizer with water 1500 times, then add "30% active ingredient content of carbendazim and oxadiazine" according to the dilution ratio of 1000 times to obtain nutrient preservative solution; take out the mesh plastic baskets and cuttings from the small arch shed one by one, soak them in the nutrient preservative solution for 10 minutes, and then cut them into the field one by one; S2.2 Flood irrigation after field cuttings: After cuttings, flood irrigation should be carried out once every day before 8 am or after 6 pm. Each time of flooding, the water should be kept for 4 to 6 hours to soak the cuttings. Flooding should be continued for 7 to 10 days until white roots are formed at the lower incision of the cuttings. Then, the number of days between flooding irrigations can be gradually extended.

2. The seabuckthorn softwood cutting method based on the two-stage method according to claim 1 is characterized in that: The construction process of the small arch shed is as follows: Level the land, pile up fences around the leveled land, insert scaffolding on the fence to form a small arch shed, dig a soil trough on the sunny side of the small arch shed, place one side of the plastic shade net and agricultural greenhouse film in the soil trough, compact and fix it with soil, and set up micro-spray pipes in the small arch shed.

3. The seabuckthorn softwood cutting method based on the two-stage method according to claim 1 is characterized in that: For the first administration, the micro-spray time is 10 to 15 minutes.

4. The seabuckthorn softwood cutting method based on the two-stage method according to claim 1, characterized in that: During the second management, the micro-spraying time is 4 to 6 hours.

5. The seabuckthorn softwood cutting method based on the two-stage method according to claim 1, characterized in that: The dimensions of the grafting slots for field cuttings are 5~10cm deep, 3~5cm wide, and the row spacing between the grafting slots is 20~30cm.

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