Ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid and preparation method and application thereof

Abstract

The present invention provides an ultra-high pressure nine-processed polygonatum, ginseng, and cordyceps militaris concentrate, and a preparation method and application thereof, and belongs to the technical field of health care products. The preparation method comprises the following steps: mixing an ultra-high pressure nine-processed polygonatum and ginseng mixture, an ultra-high pressure cordyceps militaris and Poria cocos mixture, and an ultra-high pressure roasted licorice with water and then soaking, heating and extracting, and then separating the solid and liquid, taking the liquid part, concentrating under vacuum conditions, and sterilizing to obtain an ultra-high pressure nine-processed polygonatum, ginseng, and cordyceps militaris concentrate. The present invention utilizes the wall breaking and bactericidal effects of ultra-high pressure and the correction effect of the nine-processing process on the effective ingredients in polygonatum and ginseng, and significantly improves the content of total saponins, polysaccharides, and total flavonoids in the polygonatum, ginseng, and cordyceps militaris concentrate. At the same time, the preparation method and ultra-high pressure sterilization process used in the present invention can significantly improve the shelf life of the prepared concentrate.

Description

Technical Field

[0001] The invention relates to the technical field of health products, and in particular to an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid and a preparation method and application thereof. Background Art

[0002] Ultra-high pressure technology refers to a method of subjecting raw materials or finished products to high pressure conditions of 100 to 1000 MPa for treatment. The cell walls and cell membranes of raw materials and finished products are destroyed by using an ultra-high pressure environment at room temperature, thereby achieving the effect of cell wall breaking and sterilization.

[0003] Nine steaming and nine drying, also known as nine steaming and nine exposure or nine processing, refers to the method of repeatedly processing Chinese medicinal materials by steaming and drying, mainly to correct the medicinal properties of the medicinal materials or increase the medicinal ingredients and reduce the toxic ingredients.

[0004] Polygonatum is sweet and nourishing, and has a mild effect. It enters the spleen, lungs, and meridians, and is a product that nourishes qi and yin. It nourishes yin and moistens the lungs, nourishes the kidneys and replenishes essence, and also nourishes the spleen and replenishes qi. It is a good tonic medicine, and is good at treating lung and kidney deficiency and qi and yin deficiency. Ginseng is sweet and warm, slightly bitter and not draining, and has a strong medicinal effect. It is an essential medicine for replenishing qi and strengthening the body. It is good at replenishing vital energy and qi deficiency; it is good at replenishing the qi of the spleen and lungs, and treating spleen and lung qi deficiency. It can also replenish qi and produce fluid, calm the mind, and improve intelligence, and treat thirst, thirst, restlessness, palpitations, forgetfulness, etc. Cordyceps militaris has good nourishing and health-care effects and extremely high medicinal value. It has a more obvious effect in anti-tumor, anti-pathogenic microorganisms, protecting the liver, kidneys and respiratory system, scavenging free radicals, regulating the immune system, regulating endocrine and anti-fatigue. The combination of Polygonatum, ginseng and Cordyceps militaris can mainly play the role of replenishing the kidney and improving the body's immunity.

[0005] However, in the prior art, when polygonatum, ginseng and cordyceps militaris are used together to prepare products for tonifying the kidney or improving the body's immunity, ethanol is mostly used as an extraction solvent for leaching, and the content of the effective ingredients of polygonatum, ginseng and cordyceps militaris in the prepared products is low, and the effect of tonifying the kidney and improving the body's immunity is not good. Therefore, it is necessary to provide a preparation method that can increase the content of the effective ingredients of polygonatum, ginseng and cordyceps militaris in the product. Summary of the invention

[0006] The invention aims to provide an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid and a preparation method and application thereof, so as to solve the technical problems that the existing products have low effective ingredient content and poor effects of tonifying the kidney and improving the body's immunity.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The present invention provides a method for preparing an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid, comprising the following steps:

[0009] (1) mixing polygonatum and ginseng with water and soaking them, taking the soaked polygonatum and ginseng and subjecting them to ultra-high pressure treatment, and subjecting the ultra-high pressure treated polygonatum and ginseng mixture to nine-steaming and nine-drying treatment to obtain an ultra-high pressure nine-processed polygonatum and ginseng mixture;

[0010] (2) mixing Cordyceps militaris and Poria cocos with water and then soaking them, taking the soaked Cordyceps militaris and Poria cocos and subjecting them to ultra-high pressure treatment to obtain an ultra-high pressure Cordyceps militaris and Poria cocos mixture;

[0011] (3) processing the licorice with honey and then subjecting it to ultra-high pressure treatment, refrigerating the licorice after ultra-high pressure treatment, frying and drying it to obtain ultra-high pressure roasted licorice;

[0012] (4) mixing the ultra-high pressure nine-processed polygonatum and ginseng mixture obtained in step (1), the ultra-high pressure Cordyceps militaris and Poria cocos mixture obtained in step (2), and the ultra-high pressure roasted licorice root obtained in step (3) with water and then soaking them, heating and extracting them, separating the solid and the liquid, taking the liquid part, concentrating it under vacuum conditions, and sterilizing it to obtain an ultra-high pressure nine-processed polygonatum, ginseng, and Cordyceps militaris concentrated solution.

[0013] Preferably, in step (1), the weight ratio of polygonatum to ginseng is 3 to 8:1, the amount of water used when mixed with water is 5 to 15 times the total weight of polygonatum and ginseng, and the soaking time is 8 to 16 hours; the temperature during the ultra-high pressure treatment is 10 to 40°C, the pressure is 200 to 600 MPa, and the pressure holding time is 5 to 35 minutes.

[0014] Preferably, in the nine-steaming and nine-drying process in step (1), the first steaming time is 3 to 4 hours, the second to fourth steaming time is 2.5 to 3.5 hours, the fifth to seventh steaming time is 1.5 to 2.5 hours, and the eighth to ninth steaming time is 0.5 to 1.5 hours.

[0015] Preferably, in step (2), the weight ratio of Cordyceps militaris to Poria cocos is 3-8:1, the amount of water used when mixed with water is 5-15 times the total weight of Cordyceps militaris and Poria cocos, and the soaking time is 8-16 hours; the temperature during the ultra-high pressure treatment is 10-40°C, the pressure is 200-600MPa, and the pressure holding time is 5-35min.

[0016] Preferably, the method of processing licorice with honey in step (3) is: heating the honey until small bubbles appear, then adding boiling water to mix, to obtain honey water; mixing the honey water with licorice, to obtain honey-processed licorice;

[0017] The honey is honey, and the volume ratio of the honey to boiling water is 0.8-1.2:0.8-1.2; the weight ratio of the honey to licorice is 1:3-5.

[0018] Preferably, the temperature during the ultrahigh pressure treatment in step (3) is 10-40° C., the pressure is 200-600 MPa, and the holding time is 5-35 min.

[0019] The refrigeration temperature is 3 to 5°C and the time is 10 to 14 hours;

[0020] The frying time is 10 to 20 minutes.

[0021] Preferably, in step (4), the weight ratio of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice is 8-12:8-12:0.8-1.2; when mixing with water, the amount of water used is 5-10 times the total weight of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice, and the soaking time is 8-16 hours.

[0022] Preferably, the temperature of the heating extraction is 40-90°C and the time is 48-72h;

[0023] The concentration temperature is 40-60°C;

[0024] The sterilization is ultra-high pressure sterilization, the temperature of the ultra-high pressure sterilization is 50-70°C, the pressure is 400-600MPa, and the pressure holding time is 10-30min; the sterilization can also be carried out at a temperature of 110-130°C and a time of 30-120min;

[0025] The soluble solid content in the concentrated solution is 5-60%.

[0026] The invention provides an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid prepared according to the method.

[0027] The present invention also provides an application of the ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid in preparing products for invigorating the kidney and improving immunity.

[0028] The present invention provides a method for preparing a polygonatum ginseng cordyceps militaris concentrated solution by using ultra-high pressure and nine-processing process, and utilizes the wall breaking and sterilization effect of ultra-high pressure and the correction effect of the nine-processing process on the effective ingredients in polygonatum ginseng and ginseng, thereby significantly improving the content of total saponins, polysaccharides and total flavonoids in the polygonatum ginseng cordyceps militaris concentrated solution. At the same time, the preparation method and ultra-high pressure sterilization process adopted by the present invention can significantly improve the shelf life of the prepared concentrated solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0030] Figure 1 This is a diagram of the apparatus used for ultrahigh pressure treatment. DETAILED DESCRIPTION

[0031] The present invention provides a method for preparing an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid, comprising the following steps:

[0032] (1) mixing polygonatum and ginseng with water and soaking them, taking the soaked polygonatum and ginseng and subjecting them to ultra-high pressure treatment, and subjecting the ultra-high pressure treated polygonatum and ginseng mixture to nine-steaming and nine-drying treatment to obtain an ultra-high pressure nine-processed polygonatum and ginseng mixture;

[0033] (2) mixing Cordyceps militaris and Poria cocos with water and then soaking them, taking the soaked Cordyceps militaris and Poria cocos and subjecting them to ultra-high pressure treatment to obtain an ultra-high pressure Cordyceps militaris and Poria cocos mixture;

[0034] (3) processing the licorice with honey and then subjecting it to ultra-high pressure treatment, refrigerating the licorice after ultra-high pressure treatment, frying and drying it to obtain ultra-high pressure roasted licorice;

[0035] (4) mixing the ultra-high pressure nine-processed polygonatum and ginseng mixture obtained in step (1), the ultra-high pressure Cordyceps militaris and Poria cocos mixture obtained in step (2), and the ultra-high pressure roasted licorice root obtained in step (3) with water and then soaking them, heating and extracting them, separating the solid and the liquid, taking the liquid part, concentrating it under vacuum conditions, and sterilizing it to obtain an ultra-high pressure nine-processed polygonatum, ginseng, and Cordyceps militaris concentrated solution.

[0036] In the present invention, the weight ratio of polygonatum to ginseng in step (1) is 3-8:1, preferably 4-7:1, more preferably 5-6:1, and more preferably 5:1; when mixed with water, the amount of water is 5-15 times the total weight of polygonatum and ginseng, preferably 7-13 times the total weight of polygonatum and ginseng, more preferably 9-11 times the total weight of polygonatum and ginseng, and more preferably 10 times the total weight of polygonatum and ginseng; the soaking time is 8-16 hours, preferably 10-14 hours, and further step is preferably 12h; the temperature during the ultra-high pressure treatment is 10-40°C, preferably 15-35°C, more preferably 20-30°C, more preferably 25°C, the pressure is 200-600MPa, preferably 250-550MPa, more preferably 300-500MPa, more preferably 400MPa, and the holding time is 5-35min, preferably 10-30min, more preferably 15-25min, more preferably 20min.

[0037] In the present invention, in the nine-steaming and nine-drying treatment in step (1), the first steaming time is 3 to 4 hours, preferably 3.5 hours, the second to fourth steaming time is 2.5 to 3.5 hours, preferably 3 hours, the fifth to seventh steaming time is 1.5 to 2.5 hours, preferably 2 hours, and the eighth to ninth steaming time is 0.5 to 1.5 hours, preferably 1 hour.

[0038] In the present invention, the weight ratio of Cordyceps militaris to Poria cocos in step (2) is 3-8:1, preferably 4-7:1, more preferably 5-6:1, and more preferably 5:1; when mixed with water, the amount of water is 5-15 times the total weight of Cordyceps militaris and Poria cocos, preferably 7-13 times the total weight of Cordyceps militaris and Poria cocos, more preferably 9-11 times the total weight of Cordyceps militaris and Poria cocos, and more preferably 10 times the total weight of Cordyceps militaris and Poria cocos; the soaking time is 8-16 hours, preferably 10-14 hours. , more preferably 12h; the temperature during the ultra-high pressure treatment is 10-40°C, preferably 15-35°C, more preferably 20-30°C, more preferably 25°C, the pressure is 200-600MPa, preferably 250-550MPa, more preferably 300-500MPa, more preferably 400MPa, and the holding time is 5-35min, preferably 10-30min, more preferably 15-25min, more preferably 20min.

[0039] In the present invention, the method of processing licorice with honey in step (3) is: heating the honey until small bubbles appear, adding boiling water to mix, and obtaining honey water; mixing the honey water with licorice, and obtaining honey-processed licorice;

[0040] The honey is honey, and the volume ratio of the honey to boiling water is 0.8-1.2:0.8-1.2, preferably 0.9-1.1:0.9-1.1, and more preferably 1:1; the weight ratio of the honey to licorice is 1:3-5, and preferably 1:4.

[0041] In the present invention, the temperature during the ultrahigh pressure treatment in step (3) is 10-40°C, preferably 15-35°C, more preferably 20-30°C, more preferably 25°C, the pressure is 200-600MPa, preferably 250-550MPa, more preferably 300-500MPa, more preferably 400MPa, and the holding time is 5-35min, preferably 10-30min, more preferably 15-25min, more preferably 20min;

[0042] The refrigeration temperature is 3-5°C, preferably 4°C, and the time is 10-14h, preferably 11-13h, and more preferably 12h;

[0043] The frying time is 10 to 20 minutes, preferably 12 to 18 minutes, more preferably 14 to 16 minutes, and more preferably 15 minutes.

[0044] In the present invention, the soaked Chinese medicinal materials are vacuum packaged before the ultrahigh pressure treatment in steps (1) to (3).

[0045] In the present invention, the weight ratio of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture and the ultra-high pressure roasted licorice in step (4) is 8-12:8-12:0.8-1.2, preferably 9-11:9-11:0.9-1.1, and more preferably 10:10:1; when mixed with water, the amount of water used is 5-10 times the total weight of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture and the ultra-high pressure roasted licorice, preferably 5-8 times the total weight of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture and the ultra-high pressure roasted licorice, and more preferably 6 times the total weight of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture and the ultra-high pressure roasted licorice, and the soaking time is 8-16 hours, preferably 10-14 hours, and more preferably 12 hours.

[0046] In the present invention, the temperature of the heating extraction is 40-90°C, preferably 50-80°C, more preferably 60-70°C, more preferably 65°C, and the time is 48-72h, preferably 52-68h, more preferably 56-64h, more preferably 60h;

[0047] The concentration temperature is 40-60°C, preferably 45-55°C, and more preferably 50°C;

[0048] The sterilization is ultra-high pressure sterilization, the temperature of the ultra-high pressure sterilization is 50-70°C, preferably 55-65°C, more preferably 60°C, the pressure is 400-600MPa, preferably 450-550MPa, more preferably 500MPa, and the holding time is 10-30min, preferably 15-25min, more preferably 20min; the sterilization can also be carried out at a temperature of 110-130°C and a time of 30-120min, preferably at a temperature of 115-125°C and a time of 50-100min, more preferably at a temperature of 120°C and a time of 75min;

[0049] The soluble solid content in the concentrated solution is 5 to 60%, preferably 10 to 50%, more preferably 15 to 40%, and even more preferably 30%.

[0050] The invention provides an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid prepared according to the method.

[0051] The present invention also provides an application of the ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid in preparing products for invigorating the kidney and improving immunity.

[0052] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention. Figure 1 shown.

[0053] Example 1

[0054] A method for preparing an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid, comprising the following steps:

[0055] (1) Mixing polygonatum and ginseng in a weight ratio of 3:1, washing with clean water, and then adding water 5 times the total weight of polygonatum and ginseng to soak for 8 hours, taking the soaked polygonatum and ginseng and vacuumizing them, packaging them in a high-pressure plastic bag, and performing ultra-high pressure treatment under the conditions of temperature of 10° C., pressure of 200 MPa, and pressure holding time of 5 minutes, and performing nine-steaming and nine-drying treatment on the polygonatum and ginseng mixture after ultra-high pressure treatment to obtain an ultra-high pressure nine-processed polygonatum and ginseng mixture;

[0056] The specific method of the nine-steaming and nine-drying treatment is: steam the ultra-high pressure processed polygonatum and ginseng mixture for 3.5 hours to thoroughly steam the polygonatum and ginseng, and then dry them in the sun. After nine times (the second to fourth steaming time is 3 hours, the fifth to seventh steaming time is 2 hours, and the eighth to ninth steaming time is 1 hour), the ultra-high pressure nine-processed polygonatum and ginseng mixture is obtained;

[0057] (2) mixing Cordyceps militaris and Poria cocos in a weight ratio of 3:1, washing with clean water, and then adding water 5 times the total weight of Cordyceps militaris and Poria cocos to soak for 8 hours, taking the soaked Cordyceps militaris and Poria cocos and vacuumizing them, packaging them in a high-pressure resistant plastic bag, and performing ultra-high pressure treatment at a temperature of 10° C., a pressure of 200 MPa, and a pressure holding time of 5 minutes to obtain an ultra-high pressure Cordyceps militaris and Poria cocos mixture;

[0058] (3) Pour honey into a pot and heat over low heat until small bubbles appear in the honey (stirring is required during this period; the honey is ready when it is dropped into cold water without dispersing). Then add boiling water of equal volume to the honey and mix to obtain honey water; pour the honey water into licorice slices of 4 times the weight of the honey and stir evenly to obtain honey-processed licorice;

[0059] The honey processed licorice is vacuumized and packaged in a high pressure resistant plastic bag, and then ultra-high pressure treatment is performed under the conditions of temperature of 10°C, pressure of 200MPa, and pressure holding time of 5min. The ultra-high pressure processed licorice is refrigerated at 4°C for 12h (it can be easily broken apart, and the center is full of honey water), and then fried on a low fire for 15min (fry until the color becomes darker and there are some burnt spots), and then spread out in the sun to dry, thereby obtaining ultra-high pressure roasted licorice;

[0060] (4) The ultra-high pressure processed polygonatum and ginseng mixture obtained in step (1), the ultra-high pressure cordyceps militaris and Poria cocos mixture obtained in step (2), and the ultra-high pressure roasted licorice root obtained in step (3) are mixed in a weight ratio of 10:10:1, and then water of 6 times the total weight of the ultra-high pressure processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice root is added and soaked for 12 hours, heated and extracted at a temperature of 40° C. for 48 hours, and then filtered, and the filtrate is placed in a vacuum concentrator and concentrated at 40° C., concentrated to a soluble solid content of 30%, and then packaged, and ultra-high pressure sterilized at a temperature of 60° C., a pressure of 500 MPa, and a pressure holding time of 20 minutes to obtain an ultra-high pressure processed polygonatum, ginseng, and cordyceps militaris concentrated solution.

[0061] Example 2

[0062] A method for preparing an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid, comprising the following steps:

[0063] (1) Mixing polygonatum and ginseng in a weight ratio of 8:1, washing with clean water, and then adding water 15 times the total weight of polygonatum and ginseng to soak for 16 hours, taking the soaked polygonatum and ginseng and vacuumizing them, packaging them in a high-pressure plastic bag, and performing ultra-high pressure treatment under the conditions of temperature of 40° C., pressure of 600 MPa, and pressure holding time of 35 minutes, and performing nine-steaming and nine-drying treatment on the ultra-high pressure treated polygonatum and ginseng mixture to obtain an ultra-high pressure nine-processed polygonatum and ginseng mixture;

[0064] The specific method of the nine-steaming and nine-drying treatment is: steam the ultra-high pressure processed polygonatum and ginseng mixture for 3.5 hours to thoroughly steam the polygonatum and ginseng, and then dry them in the sun. After nine times (the second to fourth steaming time is 3 hours, the fifth to seventh steaming time is 2 hours, and the eighth to ninth steaming time is 1 hour), the ultra-high pressure nine-processed polygonatum and ginseng mixture is obtained;

[0065] (2) mixing Cordyceps militaris and Poria cocos in a weight ratio of 8:1, washing with clean water, and then adding 15 times the total weight of Cordyceps militaris and Poria cocos to soak for 16 hours, taking the soaked Cordyceps militaris and Poria cocos and vacuumizing them and packaging them in a high-pressure plastic bag, and performing ultra-high pressure treatment at a temperature of 40° C., a pressure of 600 MPa, and a pressure holding time of 35 minutes to obtain an ultra-high pressure Cordyceps militaris and Poria cocos mixture;

[0066] (3) Pour honey into a pot and heat over low heat until small bubbles appear in the honey (stirring is required during this period; the honey is ready when it is dropped into cold water without dispersing). Then add boiling water of equal volume to the honey and mix to obtain honey water; pour the honey water into licorice slices of 4 times the weight of the honey and stir evenly to obtain honey-processed licorice;

[0067] The honey processed licorice is vacuumized and packaged in a high pressure resistant plastic bag, and then ultra-high pressure treatment is performed under the conditions of temperature of 40°C, pressure of 600MPa, and pressure holding time of 35min. The ultra-high pressure processed licorice is refrigerated at 4°C for 12h (it can be easily broken apart, and the center is full of honey water), and then fried on a low fire for 15min (fry until the color becomes darker and there are some burnt spots), and then spread out in the sun to dry, so as to obtain ultra-high pressure roasted licorice;

[0068] (4) The ultra-high pressure processed polygonatum and ginseng mixture obtained in step (1), the ultra-high pressure cordyceps militaris and Poria cocos mixture obtained in step (2), and the ultra-high pressure roasted licorice root obtained in step (3) are mixed in a weight ratio of 10:10:1, and then 6 times the total weight of the ultra-high pressure processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice root are added and soaked for 12 hours, heated and extracted at a temperature of 90° C. for 72 hours, and then filtered, and the filtrate is placed in a vacuum concentrator and concentrated at 60° C., concentrated to a soluble solid content of 30%, and then packaged, and sterilized at a temperature of 120° C. for 90 minutes to obtain an ultra-high pressure processed polygonatum, ginseng, and cordyceps militaris concentrated solution.

[0069] Example 3

[0070] A method for preparing an ultra-high pressure nine-processed polygonatum ginseng cordyceps militaris concentrated liquid, comprising the following steps:

[0071] (1) Mixing polygonatum and ginseng in a weight ratio of 5:1, washing with clean water, and then adding water 10 times the total weight of polygonatum and ginseng to soak for 12 hours, taking the soaked polygonatum and ginseng and vacuumizing them, packaging them in a high-pressure plastic bag, and performing ultra-high pressure treatment under the conditions of temperature of 25° C., pressure of 400 MPa, and pressure holding time of 20 minutes, and performing nine-steaming and nine-drying treatment on the ultra-high pressure treated polygonatum and ginseng mixture to obtain an ultra-high pressure nine-processed polygonatum and ginseng mixture;

[0072] The specific method of the nine-steaming and nine-drying treatment is: steam the ultra-high pressure processed polygonatum and ginseng mixture for 3.5 hours to thoroughly steam the polygonatum and ginseng, and then dry them in the sun. After nine times (the second to fourth steaming time is 3 hours, the fifth to seventh steaming time is 2 hours, and the eighth to ninth steaming time is 1 hour), the ultra-high pressure nine-processed polygonatum and ginseng mixture is obtained;

[0073] (2) mixing Cordyceps militaris and Poria cocos in a weight ratio of 5:1, washing with clean water, and then adding 10 times the total weight of Cordyceps militaris and Poria cocos to soak for 12 hours, taking the soaked Cordyceps militaris and Poria cocos, vacuuming them, packaging them in a high-pressure plastic bag, and performing ultra-high pressure treatment at a temperature of 25° C., a pressure of 400 MPa, and a pressure holding time of 20 minutes to obtain an ultra-high pressure Cordyceps militaris and Poria cocos mixture;

[0074] (3) Pour honey into a pot and heat over low heat until small bubbles appear in the honey (stirring is required during this period; the honey is ready when it is dropped into cold water without dispersing). Then add boiling water of equal volume to the honey and mix to obtain honey water; pour the honey water into licorice slices of 4 times the weight of the honey and stir evenly to obtain honey-processed licorice;

[0075] The honey processed licorice is vacuumized and packaged in a high pressure resistant plastic bag, and then ultra-high pressure treatment is performed under the conditions of temperature of 25°C, pressure of 400MPa, and pressure holding time of 20min. The ultra-high pressure processed licorice is refrigerated at 4°C for 12h (it can be easily broken apart, and the center is full of honey water), and then fried on a low fire for 15min (fry until the color becomes darker and there are some burnt spots), and then spread out in the sun to dry, so as to obtain ultra-high pressure roasted licorice;

[0076] (4) The ultra-high pressure processed polygonatum and ginseng mixture obtained in step (1), the ultra-high pressure cordyceps militaris and Poria cocos mixture obtained in step (2), and the ultra-high pressure roasted licorice root obtained in step (3) are mixed in a weight ratio of 10:10:1, and then water of 6 times the total weight of the ultra-high pressure processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice root is added and soaked for 12 hours, and the mixture is heated and extracted at a temperature of 65° C. for 60 hours, and then filtered, and the filtrate is placed in a vacuum concentrator and concentrated at 50° C., and the filtrate is concentrated to a soluble solid content of 30%, and then packaged, and ultra-high pressure sterilized at a temperature of 60° C., a pressure of 500 MPa, and a pressure holding time of 20 minutes to obtain an ultra-high pressure processed polygonatum, ginseng, and cordyceps militaris concentrated solution.

[0077] Comparative Example 1

[0078] The difference between this comparative example and Example 3 is that in this comparative example, the ultra-high pressure treated polygonatum and ginseng mixture obtained by ultra-high pressure treatment is not subjected to nine-steaming and nine-drying treatment, and the other steps are the same as Example 3 to obtain an ultra-high pressure polygonatum ginseng cordyceps militaris concentrated liquid.

[0079] Comparative Example 2

[0080] The difference between this comparative example and Example 3 is that in step (1) of this comparative example, polygonatum and ginseng are subjected to ultra-high pressure treatment and nine-steaming and nine-drying treatment respectively (the parameters of the ultra-high pressure treatment and nine-steaming and nine-drying treatment are the same as those in Example 3) to obtain ultra-high pressure nine-processed polygonatum and ultra-high pressure nine-processed ginseng, and the other steps are the same as those in Example 3.

[0081] Comparative Example 3

[0082] The difference between this comparative example and Example 3 is that in step (2) of this comparative example, Cordyceps militaris and Poria cocos are subjected to ultra-high pressure treatment respectively (parameters of ultra-high pressure treatment are the same as those in Example 3) to obtain ultra-high pressure Cordyceps militaris and ultra-high pressure Poria cocos, and the other steps are the same as those in Example 3.

[0083] Comparative Example 4

[0084] A method for preparing a concentrated solution of polygonatum, ginseng and cordyceps militaris, comprising the following steps:

[0085] (1) Pour honey into a pot and heat over low heat until small bubbles appear in the honey (stirring is required during this period; the honey is ready when it is dropped into cold water without dispersing). Then add boiling water of equal volume to the honey and mix to obtain honey water. Pour the honey water into licorice slices of 4 times the weight of the honey and stir evenly to obtain honey-processed licorice. Refrigerate the honey-processed licorice at 4°C for 12 hours (it can be easily broken apart and the center is fully absorbed by the honey water). Then stir-fry over low heat for 15 minutes (until the color becomes darker and there are some burnt spots). Spread it out in the sun to dry to obtain roasted licorice.

[0086] (2) Mix polygonatum, ginseng, cordyceps militaris, poria cocos and licorice root in a weight ratio of 50:10:50:10:6 and grind them, pass through a 40-mesh sieve, take the sieve-undersized material and add 95% ethanol in an amount 10 times the weight of the sieve-undersized material, extract twice at a pressure of 40 MPa, each extraction time for 2 hours, combine the two extracts, concentrate under vacuum at a temperature of 50° C., concentrate until the soluble solid content in the filtrate is 30%, then package and sterilize at a temperature of 120° C. for 90 minutes to obtain a polygonatum ginseng cordyceps militaris concentrated solution.

[0087] Test Example 1

[0088] The contents of total saponins, polysaccharides and total flavonoids in the concentrated solutions prepared in Examples 1 to 3 and Comparative Examples 1 to 4 were measured respectively. The total saponin content was measured by vanillin-perchloric acid colorimetry, the polysaccharide content was measured by phenol-sulfuric acid method, and the total flavonoid content was measured by sodium nitrite-aluminum nitrate colorimetry. The measurement results are shown in Table 1.

[0089] Table 1 Contents of total saponins, polysaccharides and total flavonoids in the concentrate

[0090]

[0091]

[0092] It can be seen from Table 1 that the contents of total saponin, polysaccharide and total flavonoids in the concentrated solutions prepared in Examples 1-3 are all higher than those in Comparative Examples 1-4. Compared with the concentrated solution obtained by the comparative example of nine-steaming and nine-drying treatment of the ultra-high pressure treated mixture of polygonatum and ginseng obtained without ultra-high pressure treatment, the present invention significantly increases the contents of total saponins, polysaccharides and total flavonoids in the concentrated solution by the method of first ultra-high pressure treatment and then nine-processing; compared with comparative example 2, the contents of total saponins, polysaccharides and total flavonoids in the concentrated solution prepared by ultra-high pressure treatment and nine-processing of polygonatum and ginseng in the present invention are significantly higher than those in the concentrated solution prepared by ultra-high pressure treatment and nine-processing of polygonatum and ginseng respectively, which is speculated to be due to the tonic effect of polygonatum and ginseng on each other during the ultra-high pressure and nine-processing treatment; compared with comparative example 3, the contents of total saponins, polysaccharides and total flavonoids in the concentrated solution prepared by ultra-high pressure treatment of Cordyceps militaris and Poria cocos in the present invention are significantly higher than those in the concentrated solution prepared by ultra-high pressure treatment of Cordyceps militaris and Poria cocos respectively; compared with comparative example 4, the method of the present invention can significantly increase the contents of total saponins, polysaccharides and total flavonoids in the concentrated solution compared with the traditional ethanol extraction method, indicating that the method of the present invention can significantly increase the content of effective ingredients in the concentrated solution compared with the traditional method.

[0093] Test Example 2

[0094] The shelf life of the concentrated solutions prepared in Examples 1 to 3 and Comparative Examples 1 to 4 was measured respectively, and the measuring method was carried out according to the method recommended in T / CNFIA 001-2017 "General Guidelines for the Shelf Life of Food". The specific method is: the samples are stored in two constant temperature boxes at 5°C and 50°C (1 month is equivalent to 12 months, and each month is calculated as 30 days), the sample at 5°C is used as a standard sample or a control sample, and the sample at 50°C is used as an environmentally destructive sample. The samples at 50°C are evaluated every 5 days and compared with the samples at 5°C during the evaluation. When the sample at 50°C has a large difference from the sample at 5°C or an unacceptable difference, the sample at 50°C stops the experiment, and the time obtained by multiplying the storage time of the sample at 50°C by 12 is the maximum shelf life of the product. The measurement results are shown in Table 2.

[0095] Table 2 Determination results of the shelf life of the concentrate

[0096]

[0097]

[0098] It can be seen from Table 2 that, compared with Example 2, the shelf life of the concentrate prepared by the ultra-high pressure sterilization method in Examples 1 and 3 of the present invention is significantly longer than that of the concentrate prepared by the sterilization method at a temperature of 120°C. It can be seen that the ultra-high pressure sterilization in Examples 1 and 3 of the present invention at a lower sterilization temperature also achieves the effect of killing the bacteria (such as Bacillus) that cause corruption in the concentrate; compared with Comparative Example 4, the preparation method and sterilization method of the concentrate of the present invention can significantly improve the shelf life of the product.

[0099] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing an ultra-high pressure nine-processed polygonatum, ginseng, and cordyceps militaris concentrated solution, characterized in that: The steps include: (1) mixing polygonatum and ginseng with water and soaking them, taking the polygonatum and ginseng after soaking and subjecting them to ultra-high pressure treatment, and subjecting the mixture of polygonatum and ginseng after ultra-high pressure treatment to nine-steaming and nine-drying treatment to obtain an ultra-high pressure nine-processed polygonatum and ginseng mixture; (2) mixing Cordyceps militaris and Poria cocos with water and soaking them, and subjecting the soaked Cordyceps militaris and Poria cocos to ultra-high pressure treatment to obtain an ultra-high pressure Cordyceps militaris and Poria cocos mixture; (3) processing licorice with honey and then subjecting it to ultra-high pressure treatment, refrigerating the ultra-high pressure licorice, frying it and drying it to obtain ultra-high pressure roasted licorice; (4) The ultra-high pressure nine-processed polygonatum and ginseng mixture obtained in step (1), the ultra-high pressure Cordyceps militaris and Poria cocos mixture obtained in step (2), and the ultra-high pressure roasted licorice root obtained in step (3) are mixed with water and then soaked, heated and extracted, and then the solid and liquid are separated. The liquid portion is taken, concentrated under vacuum conditions, and sterilized to obtain an ultra-high pressure nine-processed polygonatum, ginseng, and Cordyceps militaris concentrated solution.

2. The method according to claim 1, characterized in that In step (1), the weight ratio of polygonatum to ginseng is 3-8:1, the amount of water used when mixed with water is 5-15 times the total weight of polygonatum and ginseng, and the soaking time is 8-16 h; the temperature during the ultra-high pressure treatment is 10-40°C, the pressure is 200-600 MPa, and the pressure holding time is 5-35 min.

3. The method according to claim 2, characterized in that In the nine-steaming and nine-drying process in step (1), the first steaming time is 3-4 h, the second to fourth steaming time is 2.5-3.5 h, the fifth to seventh steaming time is 1.5-2.5 h, and the eighth to ninth steaming time is 0.5-1.5 h.

4. The method according to claim 3, characterized in that In step (2), the weight ratio of Cordyceps militaris to Poria cocos is 3-8:1, the amount of water used when mixed with water is 5-15 times the total weight of Cordyceps militaris and Poria cocos, and the soaking time is 8-16 h; the temperature during the ultra-high pressure treatment is 10-40°C, the pressure is 200-600 MPa, and the pressure holding time is 5-35 min.

5. The method according to claim 4, characterized in that Step (3) The method for preparing licorice with honey is as follows: heating the honey until small bubbles appear, then adding boiling water to mix, to obtain honey water; mixing the honey water with licorice, to obtain honey-prepared licorice; The honey is honey, and the volume ratio of the honey to boiling water is 0.8-1.2:0.8-1.2; the weight ratio of the honey to licorice is 1:3-5.

6. The method according to claim 5, characterized in that The temperature during the ultrahigh pressure treatment in step (3) is 10-40°C, the pressure is 200-600 MPa, and the holding time is 5-35 min; The refrigeration temperature is 3-5°C and the time is 10-14 h; The frying time is 10 to 20 min.

7. The method according to claim 6, characterized in that In step (4), the weight ratio of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice is 8-12:8-12:0.8-1.2; when mixing with water, the amount of water used is 5-10 times the total weight of the ultra-high pressure nine-processed polygonatum and ginseng mixture, the ultra-high pressure cordyceps militaris and Poria cocos mixture, and the ultra-high pressure roasted licorice, and the soaking time is 8-16 h.

8. The method according to any one of claims 1 to 7, characterized in that The heating extraction temperature is 40-90°C and the time is 48-72 h; The concentration temperature is 40-60°C; The sterilization is ultra-high pressure sterilization, the temperature of the ultra-high pressure sterilization is 50-70°C, the pressure is 400-600MPa, and the pressure holding time is 10-30 min; or the sterilization is carried out at a temperature of 110-130°C and a time of 30-120 min; The soluble solid content in the concentrated solution is 5-60%.

9. An ultra-high pressure nine-processed polygonatum, ginseng, cordyceps militaris concentrated liquid prepared according to the method of any one of claims 1 to 8.

10. Use of the ultra-high pressure nine-processed polygonatum, ginseng, cordyceps militaris concentrated liquid as claimed in claim 9 in the preparation of products for tonifying the kidney and improving immunity.

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