Preparation method of a composition for removing eye bags and fading dark circles, and its preparation and application

Abstract

The invention relates to a preparation method of a composition for removing eye bags and fading dark circles, and its preparation and application, belonging to the technical field of cosmetics. The method adopts smilax chinaberry kernel and papaya kernel as composite raw materials, and obtains a small molecule composite extract below 2 kDa through multi-stage enzymolysis with lipase, papain, serrapeptase and ficin; further adopts sanguinea vine leaf, loblolly leaf and alfalfa leaf as composite raw materials, and obtains a small molecule composite extract below 2 kDa through multi-stage enzymolysis with hemicellulase, bromelain and pepsin; the composite use of the components has a synergistic effect, can well improve dark circles and reduce the volume of eye bags, and the various extracts contain small molecule active substances and small molecule peptide substances, which can promote cells to synthesize collagen, restore skin elasticity, and improve cell metabolism and detoxification capabilities.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a preparation method of a composition for removing eye bags and fading dark circles, and a preparation and application thereof. Background Art

[0002] Eye bags can be divided into two categories: congenital and acquired. Congenital eye bags can only be corrected by surgery, while most people's eye bags are acquired and can be alleviated by conservative treatment. Acquired eye bags are generally caused by edema of the connective tissue under the eyes due to irregular lifestyle, excessive smoking and drinking, and staying up late, as well as sagging facial skin with aging. In addition, staying up late often causes dark circles under the eyes.

[0003] With the acceleration of modern life pace, the increase of life pressure, and the richness of nightlife, for young people, irregular life, frequent staying up late, eye fatigue are common phenomena, and the occurrence of eye bags and eye circles caused by lower eyelid edema is also increasing, commonly known as "panda eyes". Due to the slow blood flow rate of venous blood vessels, insufficient oxygen supply to the red blood cells in the eye skin, excessive accumulation of carbon dioxide and metabolic waste in the veins, chronic hypoxia, dark blood and stagnation, and eye pigmentation, congestion and edema of the skin around the eyes, seriously affecting the beauty of the face. In order to meet the needs of consumers, various products for removing eye bags and dark circles have appeared on the market, mainly to improve eye bags and dark circles from the aspects of whitening, lightening spots, and reducing swelling. The effects are also uneven, and there are generally problems such as long treatment course, slow effect, and unobvious effect. Especially when the user does not change his living habits, the use of ordinary products for removing eye bags and dark circles is a drop in the bucket and cannot achieve a good relief effect. Summary of the invention

[0004] The existing products for removing eye bags and dark circles still have problems such as unsatisfactory effects, long treatment courses, slow effects, and unclear effects. The present invention provides a preparation method for a composition for removing eye bags and lightening dark circles, as well as its preparation and application, using Rhizoma Cyperi, papaya seeds, Cyperi saccharifolia leaves, Bambusa longituba leaves and alfalfa leaves as main raw materials, Rhizoma Cyperi and papaya seeds are subjected to a multi-stage composite enzymatic hydrolysis method to obtain a small molecule composite extract, and Cyperi saccharifolia leaves, Bambusa longituba leaves and alfalfa leaves are subjected to a multi-stage enzymatic hydrolysis method to obtain a small molecule composite extract, and the composite use of each component has a synergistic effect, and the combined use can well improve dark circles and reduce the volume of eye bags, and the various extracts contain small molecule active substances and small molecule peptide substances, which can promote cells to synthesize collagen, restore skin elasticity, and improve cell metabolism and detoxification capabilities. The specific technical scheme is as follows:

[0005] A method for preparing a composition for removing eye bags and fading dark circles comprises the following steps:

[0006] S1. Preparation of composite extract I:

[0007] According to the mass ratio, the kernel of the safflower seed: the kernel of papaya seed = (3-5): (1-2), the kernel of the safflower seed and the kernel of papaya seed are mixed and crushed to obtain a mixture A, water is added to the mixture A, the pH is adjusted to 7.2-7.8, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and the mixture is enzymatically hydrolyzed at 32-38°C for 4-5h, the temperature is raised to 50-60°C, the pH is adjusted to 5.0-7.0, papain is added, and the enzymatic hydrolysis is continued for 2-3h, and then the enzyme is inactivated in a water bath, the temperature is lowered to 45-50°C, the pH is adjusted to 5.5-6.0, serrapeptase is added for enzymatic hydrolysis for 1h-1.5h, the temperature is raised to 60-65°C, ficin is added, the enzymatic hydrolysis is continued for 1h-1.5h, the enzyme is inactivated in a water bath, the temperature is lowered to room temperature, and a 2kDa ultrafiltration membrane is used to obtain a liquid below 2kDa, which is spray-dried to obtain a composite extract I;

[0008] S2, Preparation of composite extract II:

[0009] According to the mass ratio, the leaves of Sanguinea sphaerocephala: the leaves of Psoralea corylifolia: the leaves of Alfalfa = (3-5): (1-2.5): (0.5-2), the leaves of Sanguinea sphaerocephala, the leaves of Psoralea corylifolia and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, water is added to the mixture B, the pH is adjusted to 4.0-5.5, hemicellulase is added, and the mixture is hydrolyzed at 50-60°C for 2h-3h, and then the enzyme is inactivated by water bath, and after the mixture is cooled to 50-55°C, the pH is adjusted to 6.0-8.0, bromelain is added, and the mixture is hydrolyzed for 1.5h-2h, and the temperature is cooled to 35-37°C, and the pH is adjusted to 1.8-2.2, and pepsin is added, and the mixture is hydrolyzed for 1h-1.5h, and the enzyme is inactivated by water bath, and after the mixture is cooled to room temperature, it is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray dried to obtain a composite extract II;

[0010] S3, compounding of the composition:

[0011] 1 to 1.5 parts of compound extract I, 1 to 1.5 parts of compound extract II, 0.5 to 1 part of hyaluronic acid, 0.3 to 0.8 parts of sorbitol, and 20 to 30 parts of purified water are mixed in parts by mass to obtain a composition for removing eye bags and lightening dark circles.

[0012] In S1 of the above preparation method, the amount of water added is 8 to 12 times the mass of the mixture A.

[0013] In S1 of the above preparation method, the added amount of the triacylglycerol lipase is 0.5% to 1.2% of the mass of the mixture A; the added amount of the diacylglycerol lipase is 0.5% to 1.2% of the mass of the mixture A; and the added amount of the monoacylglycerol lipase is 0.5% to 1.2% of the mass of the mixture A.

[0014] In S1 of the above preparation method, the amount of papain added is 1% to 3% of the mass of mixture A; the amount of serrapeptase added is 0.5% to 1% of the mass of mixture A; and the amount of ficin added is 0.5% to 1% of the mass of mixture A.

[0015] In S1 of the above preparation method, the amount of water added is 8 to 12 times the mass of the mixture B.

[0016] In S1 of the above preparation method, the amount of hemicellulase added is 1% to 3% of the mass of mixture B; the amount of bromelain added is 0.5% to 1.5% of the mass of mixture B; and the amount of pepsin added is 0.5% to 1.5% of the mass of mixture B.

[0017] In S1 and S2 of the above preparation method, the temperature of the water bath inactivation of the enzyme is 85° C. to 100° C., and the time is 25 min to 40 min.

[0018] In S3 of the above preparation method, the mixing temperature is 30°C to 45°C.

[0019] A composition preparation for removing eye bags and fading dark circles, the preparation is prepared from the following raw materials in parts by mass: 10 to 20 parts of the composition for removing eye bags and fading dark circles, 0.3 to 1 part of lecithin, 0.05 to 0.15 parts of sorbic acid, 0.3 to 0.8 parts of sodium alginate, 0.5 to 1.2 parts of carbomer, and 6 to 12 parts of purified water.

[0020] The above-mentioned composition preparation for removing eye bags and lightening dark circles is used for preparing cosmetics for removing eye bags and dark circles.

[0021] The preparation method, preparation and application of a composition for removing eye bags and lightening dark circles provided by the present invention have the following beneficial effects compared with the prior art:

[0022] 1. Eye bags are a phenomenon of metabolic obstruction, and are often accompanied by dark yellow and purple skin in the eye bag area, and even develop into dark circles. The composition for removing eye bags and fading dark circles of the present invention contains a composite extract of Rhizoma Cyperi-Papaya Seed (composite extract I), a composite extract of Caulis Spatholobi-Pendula-Medicago truncatula leaf (composite extract II), hyaluronic acid, sorbitol and purified water; each component is reasonably matched in a certain ratio, and can promote the expression of heme oxygenase-1 gene, improve the condition of eye bags and dark circles, and quickly reduce the volume of eye bags, detoxify, reduce inflammation and reduce swelling.

[0023] 2. The kernel of the Chinese prickly ash has a good effect in treating red, swollen and painful eyes, red and sore eyelids, and dark eyes and photophobia; the seed of papaya has the effects of anti-aging, anti-inflammatory, detumescence, activating blood circulation, promoting blood circulation, and repairing skin macular degeneration. The present invention adopts koreana kernel and papaya kernel as composite raw materials, and catalyzes and hydrolyzes oils and fats by triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase to generate fatty acids, glycerol and monoglyceride or diester, thereby increasing the richness of active ingredients, facilitating skin absorption and rapidly improving skin condition; in addition, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase can also serve as enzymatic substrates of papain, and hydrolyze to generate small molecule peptides and amino acids, which are active ingredients for improving eye bags and dark circles of the composition, and papain also has the function of further decomposing fat to obtain rich active substances; then, the papain enzymatic hydrolysis composite product is enzymatically hydrolyzed by serrapeptase, and finally enzymatically hydrolyzed by ficin to extract small molecule composite components below 2 kDa, which has a good effect on removing eye bags and dark circles, and is more excellent and prominent in improving eye bags and dark circles compared with simplified extraction steps.

[0024] 3. The leaves of Sanguinea strychnifolia have the effects of dispelling wind, activating blood circulation, promoting blood circulation, and anti-oxidation; the leaves of Psoralea corylifolia have the effects of disinfection, detumescence, anti-inflammation, analgesia, and anti-oxidation; the leaves of Alfalfa have the effects of diuresis, detumescence, anti-oxidation, and endocrine improvement, and are commonly used to treat urinary system disorders. The present invention uses the leaves of Sanguinea strychnifolia, Psoralea corylifolia, and Alfalfa leaves as composite raw materials, and extracts small molecule composite components below 2kDa through graded enzymolysis by hemicellulase, bromelain, and pepsin. The components contain small molecule plant peptides, amino acids, and other small molecule active ingredients, which are more easily absorbed by the skin of eye bags, and the components have a good effect of improving eye bags and dark circles; compared with the product of omitting the extraction step, it has a more efficient effect. The reasonable proportion of the enzymatic extracts of three plant leaves and the enzymatic extracts of two plant kernels has a good synergistic effect, which can quickly and efficiently improve the symptoms of eye bags and dark circles.

[0025] 4. Remove eye bags and lighten dark circles. Hyaluronic acid, sorbitol and other ingredients are added to the composition to improve moisturizing and skin conditioning effects.

[0026] 5. The present invention adopts the eye bag removal and dark circle lightening composition to prepare a cosmetic product (eye bag removal and dark circle lightening composition preparation), which contains 10 to 20 parts of the eye bag removal and dark circle lightening composition, 0.3 to 1 part of lecithin, 0.05 to 0.15 parts of sorbic acid, 0.3 to 0.8 parts of sodium alginate, 0.5 to 1.2 parts of carbomer, and 6 to 12 parts of purified water. After 3 months of application on the eye bag area, the I TA° value is significantly improved, the effect of improving the skin color of the eye bag is good, and the volume of the eye bag is greatly reduced. The use of extracts of rutin kernel, papaya seed, sanguinea vine leaf, light bamboo leaf and alfalfa leaf can well improve dark circles and reduce the volume of eye bags. Various extracts contain small molecule active substances and small molecule peptide substances, which can promote cell synthesis of collagen, restore skin elasticity, and improve cell metabolism and detoxification capabilities. Among them, the extracts of Sanguinea chinensis leaves, Bambusa truncatula leaves and Alfalfa leaves have the ability to promote blood circulation and reduce swelling, and have a good effect in removing eye bags; the extracts of Rhizoma Cyperi, Papaya seeds, Bambusa truncatula leaves and Alfalfa leaves have good antioxidant and anti-aging effects, reduce wrinkles, lift the skin, and thus delay the development of eye bags. DETAILED DESCRIPTION

[0027] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0028] Example 1

[0029] A method for preparing a composition for removing eye bags and fading dark circles comprises the following steps:

[0030] S1. Preparation of composite extract I:

[0031] According to the mass ratio of kudzu kernel: papaya kernel=4:1.5, kudzu kernel and papaya kernel are mixed and crushed to obtain mixture A, 10 times the mass of water is added to mixture A, the pH is adjusted to 7.5, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and after enzymatic hydrolysis at 35°C for 4.5 hours, the temperature is raised to 55°C, the pH is adjusted to 6.0, papain is added, and enzymatic hydrolysis is continued for 2.5 hours, and then the enzyme is inactivated in a water bath at 90°C for 30 minutes, the temperature is lowered to 48°C, the pH is adjusted to 5.8, serrapeptase is added for enzymatic hydrolysis for 1 hour, the temperature is raised to 62°C, ficin is added, the enzyme is inactivated in a water bath at 90°C for 30 minutes, the temperature is lowered to room temperature, and a 2kDa ultrafiltration membrane is used to obtain a liquid below 2kDa, which is spray-dried to obtain composite extract I;

[0032] Among them, the added amount of triacylglycerol lipase is 0.8% of the mass of mixture A; the added amount of diacylglycerol lipase is 0.8% of the mass of mixture A; the added amount of monoacylglycerol lipase is 1% of the mass of mixture A; the added amount of papain is 2% of the mass of mixture A; the added amount of serrapeptase is 0.8% of the mass of mixture A; and the added amount of ficin is 0.6% of the mass of mixture A.

[0033] S2, Preparation of composite extract II:

[0034] According to the mass ratio, the ratio of Sanguinea spatholobi leaves: bamboo leaves: alfalfa leaves is 4:2:1. The Sanguinea spatholobi leaves, bamboo leaves and alfalfa leaves are mixed and crushed to obtain a mixture B. 10 times the mass of water is added to the mixture B, the pH is adjusted to 5.0, hemicellulase is added, and the mixture is hydrolyzed at 55°C for 2.5 hours. Then, the enzyme is inactivated in a water bath at 90°C for 30 minutes. After the mixture is cooled to 52°C, the pH is adjusted to 7.0, bromelain is added, and the mixture is hydrolyzed for 1.5 hours. The mixture is cooled to 36°C, the pH is adjusted to 2.0, pepsin is added, and the mixture is hydrolyzed for 1 hour. The mixture is inactivated in a water bath at 90°C for 30 minutes. After the mixture is cooled to room temperature, it is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, which is spray-dried to obtain a composite extract II.

[0035] Wherein, the added amount of hemicellulase is 2% of the mass of mixture B; the added amount of bromelain is 1% of the mass of mixture B; and the added amount of pepsin is 1% of the mass of mixture B.

[0036] S3, compounding of the composition:

[0037] In parts by mass, 1.2 parts of compound extract I, 1.2 parts of compound extract II, 0.6 parts of hyaluronic acid, 0.5 parts of sorbitol and 25 parts of purified water are mixed at 40° C. to obtain a composition for removing eye bags and lightening dark circles.

[0038] A composition preparation for removing eye bags and fading dark circles is prepared from the following raw materials in parts by mass: 15 parts of the composition for removing eye bags and fading dark circles, 0.5 parts of lecithin, 0.1 parts of sorbic acid, 0.5 parts of sodium alginate, 0.7 parts of carbomer, and 8 parts of purified water.

[0039] The above-mentioned composition preparation for removing eye bags and lightening dark circles is used for preparing cosmetics for removing eye bags and dark circles.

[0040] Example 2

[0041] A method for preparing a composition for removing eye bags and fading dark circles comprises the following steps:

[0042] S1. Preparation of composite extract I:

[0043] According to the mass ratio of rutin kernel: papaya kernel=3:2, rutin kernel and papaya kernel are mixed and crushed to obtain mixture A, 8 times the mass of water is added to mixture A, the pH is adjusted to 7.8, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and after enzymatic hydrolysis at 32°C for 5h, the temperature is raised to 50°C, the pH is adjusted to 7.0, papain is added, and enzymatic hydrolysis is continued for 2h, and then the enzyme is inactivated in a water bath at 100°C for 25min, the temperature is lowered to 50°C, the pH is adjusted to 5.5, serrapeptase is added for enzymatic hydrolysis for 1.5h, the temperature is raised to 60°C, ficin is added, the enzyme is inactivated in a water bath at 85°C for 40min, the temperature is lowered to room temperature, and a 2kDa ultrafiltration membrane is used to obtain a liquid below 2kDa, which is spray-dried to obtain composite extract I;

[0044] Among them, the added amount of triacylglycerol lipase is 0.5% of the mass of mixture A; the added amount of diacylglycerol lipase is 1.2% of the mass of mixture A; the added amount of monoacylglycerol lipase is 0.5% of the mass of mixture A; the added amount of papain is 3% of the mass of mixture A; the added amount of serrapeptase is 0.5% of the mass of mixture A; and the added amount of ficin is 1% of the mass of mixture A.

[0045] S2, Preparation of composite extract II:

[0046] According to the mass ratio, the leaves of Sanguinea sphaerocephala: the leaves of Bambusa longituba: the leaves of Alfalfa = 3:2.5:0.5, the leaves of Sanguinea sphaerocephala, the leaves of Bambusa longituba and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, 12 times the mass of water is added to the mixture B, the pH is adjusted to 4.0, hemicellulase is added, and the mixture is hydrolyzed at 60°C for 2h, and then the enzyme is inactivated in a water bath at 100°C for 25min, and after the mixture is cooled to 55°C, the pH is adjusted to 6.0, bromelain is added, and the mixture is hydrolyzed for 2h, and the temperature is cooled to 35°C, and the pH is adjusted to 2.2, pepsin is added, and the mixture is hydrolyzed for 1h, and the enzyme is inactivated in a water bath at 100°C for 25min, and after the mixture is cooled to room temperature, it is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray-dried to obtain a composite extract II;

[0047] The amount of hemicellulase added is 3% of the mass of the mixture B; the amount of bromelain added is 0.5% of the mass of the mixture B; and the amount of pepsin added is 1.5% of the mass of the mixture B.

[0048] S3, compounding of the composition:

[0049] In parts by mass, 1 part of compound extract I, 1.5 parts of compound extract II, 0.5 parts of hyaluronic acid, 0.3 parts of sorbitol, and 30 parts of purified water are mixed at 30° C. to obtain a composition for removing eye bags and lightening dark circles.

[0050] A composition preparation for removing eye bags and fading dark circles, the preparation is prepared from the following raw materials in parts by mass: 20 parts of the composition for removing eye bags and fading dark circles, 0.3 parts of lecithin, 0.15 parts of sorbic acid, 0.3 parts of sodium alginate, 1.2 parts of carbomer, and 10 parts of purified water.

[0051] The above-mentioned composition preparation for removing eye bags and lightening dark circles is used for preparing cosmetics for removing eye bags and dark circles.

[0052] Example 3

[0053] A method for preparing a composition for removing eye bags and fading dark circles comprises the following steps:

[0054] S1. Preparation of composite extract I:

[0055] According to the mass ratio of rutin kernel: papaya kernel=3:1, rutin kernel and papaya kernel are mixed and crushed to obtain mixture A, 8 times the mass of water is added to mixture A, the pH is adjusted to 7.2, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and after enzymatic hydrolysis at 32°C for 4 hours, the temperature is raised to 50°C, the pH is adjusted to 5.0, papain is added, and enzymatic hydrolysis is continued for 2 hours, and then the enzyme is inactivated in a water bath at 85°C for 25 minutes, the temperature is lowered to 45°C, the pH is adjusted to 5.5, serrapeptase is added for enzymatic hydrolysis for 1 hour, the temperature is raised to 60°C, ficin is added, the enzyme is inactivated in a water bath at 85°C for 25 minutes, the temperature is lowered to room temperature, and a 2kDa ultrafiltration membrane is used to filter to obtain a liquid below 2kDa, which is spray-dried to obtain composite extract I;

[0056] Among them, the added amount of triacylglycerol lipase is 0.5% of the mass of mixture A; the added amount of diacylglycerol lipase is 0.5% of the mass of mixture A; the added amount of monoacylglycerol lipase is 0.5% of the mass of mixture A; the added amount of papain is 1% of the mass of mixture A; the added amount of serrapeptase is 0.5% of the mass of mixture A; and the added amount of ficin is 0.5% of the mass of mixture A.

[0057] S2, Preparation of composite extract II:

[0058] According to the mass ratio, the leaves of Sanguinea strychnifolia: the leaves of Bambusa longituba: the leaves of Alfalfa = 3:1:0.5, the leaves of Sanguinea strychnifolia, the leaves of Bambusa longituba and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, 8 times the mass of water is added to the mixture B, the pH is adjusted to 4.0, hemicellulase is added, and the mixture is hydrolyzed at 50°C for 2h, and then the enzyme is inactivated in a water bath at 85°C for 25min, after the mixture is cooled to 50°C, the pH is adjusted to 6.0, bromelain is added, the mixture is hydrolyzed for 1.5h, the temperature is cooled to 35°C, the pH is adjusted to 1.8, pepsin is added, the mixture is hydrolyzed for 1h, the enzyme is inactivated in a water bath at 85°C for 25min, the mixture is cooled to room temperature, and a 2kDa ultrafiltration membrane is used for filtration to obtain a liquid below 2kDa, which is spray-dried to obtain a composite extract II;

[0059] The amount of hemicellulase added is 1% of the mass of the mixture B; the amount of bromelain added is 0.5% of the mass of the mixture B; and the amount of pepsin added is 0.5% of the mass of the mixture B.

[0060] S3, compounding of the composition:

[0061] According to the mass proportions, 1 part of compound extract I, 1 part of compound extract II, 0.5 part of hyaluronic acid, 0.3 part of sorbitol and 20 parts of purified water are mixed at 30° C. to obtain a composition for removing eye bags and lightening dark circles.

[0062] A composition preparation for removing eye bags and fading dark circles, the preparation is prepared from the following raw materials in parts by mass: 10 parts of the composition for removing eye bags and fading dark circles, 0.3 parts of lecithin, 0.05 parts of sorbic acid, 0.3 parts of sodium alginate, 0.5 parts of carbomer, and 6 parts of purified water.

[0063] The above-mentioned composition preparation for removing eye bags and lightening dark circles is used for preparing cosmetics for removing eye bags and dark circles.

[0064] Example 4

[0065] A method for preparing a composition for removing eye bags and fading dark circles comprises the following steps:

[0066] S1. Preparation of composite extract I:

[0067] According to the mass ratio of rutin kernel: papaya kernel=5:1, rutin kernel and papaya kernel are mixed and crushed to obtain mixture A, 12 times the mass of water is added to mixture A, the pH is adjusted to 7.2, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and after enzymatic hydrolysis at 38°C for 4h, the temperature is raised to 60°C, the pH is adjusted to 5.0, papain is added, and enzymatic hydrolysis is continued for 3h, and then the enzyme is inactivated in a water bath at 85°C for 40min, the temperature is lowered to 45°C, the pH is adjusted to 6.0, serrapeptase is added for enzymatic hydrolysis for 1h, the temperature is raised to 65°C, ficin is added, the enzyme is inactivated in a water bath at 100°C for 25min, the temperature is lowered to room temperature, and a liquid below 2kDa is obtained by spray drying to obtain composite extract I;

[0068] Among them, the added amount of triacylglycerol lipase is 1.2% of the mass of mixture A; the added amount of diacylglycerol lipase is 0.5% of the mass of mixture A; the added amount of monoacylglycerol lipase is 1.2% of the mass of mixture A; the added amount of papain is 1% of the mass of mixture A; the added amount of serrapeptase is 1% of the mass of mixture A; and the added amount of ficin is 0.5% of the mass of mixture A.

[0069] S2, Preparation of composite extract II:

[0070] According to the mass ratio, the leaves of Sanguinea strychnifolia: the leaves of Bambusa longituba: the leaves of Alfalfa = 5:1:2, the leaves of Sanguinea strychnifolia, the leaves of Bambusa longituba and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, 8 times the mass of water is added to the mixture B, the pH is adjusted to 5.5, hemicellulase is added, and the mixture is hydrolyzed at 50°C for 3 hours, and then the enzyme is inactivated in a water bath at 85°C for 40 minutes, and after the mixture is cooled to 50°C, the pH is adjusted to 8.0, bromelain is added, and the mixture is hydrolyzed for 1.5 hours, and the temperature is cooled to 37°C, and the pH is adjusted to 1.8, pepsin is added, and the mixture is hydrolyzed for 1.5 hours, and the enzyme is inactivated in a water bath at 85°C for 40 minutes, and after the mixture is cooled to room temperature, it is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray-dried to obtain a composite extract II;

[0071] The amount of hemicellulase added is 1% of the mass of the mixture B; the amount of bromelain added is 1.5% of the mass of the mixture B; and the amount of pepsin added is 0.5% of the mass of the mixture B.

[0072] S3, compounding of the composition:

[0073] According to the mass proportions, 1.5 parts of compound extract I, 1 part of compound extract II, 1 part of hyaluronic acid, 0.8 parts of sorbitol and 20 parts of purified water are mixed at 45° C. to obtain a composition for removing eye bags and lightening dark circles.

[0074] A composition preparation for removing eye bags and fading dark circles, the preparation is prepared from the following raw materials in parts by mass: 10 parts of the composition for removing eye bags and fading dark circles, 1 part of lecithin, 0.05 parts of sorbic acid, 0.8 parts of sodium alginate, 0.5 parts of carbomer, and 12 parts of purified water.

[0075] The above-mentioned composition preparation for removing eye bags and lightening dark circles is used for preparing cosmetics for removing eye bags and dark circles.

[0076] Example 5

[0077] A method for preparing a composition for removing eye bags and fading dark circles comprises the following steps:

[0078] S1. Preparation of composite extract I:

[0079] According to the mass ratio, the rutin kernel: papaya kernel = 5:2, the rutin kernel and the papaya kernel are mixed and crushed to obtain a mixture A, 12 times the mass of water is added to the mixture A, the pH is adjusted to 7.8, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and the mixture is enzymatically hydrolyzed at 38°C for 5 hours, the temperature is raised to 60°C, the pH is adjusted to 7.0, papain is added, and the enzymatic hydrolysis is continued for 3 hours, and then the enzyme is inactivated in a water bath at 100°C for 40 minutes, the temperature is lowered to 50°C, the pH is adjusted to 6.0, serrapeptase is added for enzymatic hydrolysis for 1.5 hours, the temperature is raised to 65°C, ficin is added, the enzyme is inactivated in a water bath at 100°C for 40 minutes, the mixture is cooled to room temperature, and filtered with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, which is spray-dried to obtain a composite extract I;

[0080] Among them, the added amount of triacylglycerol lipase is 1.2% of the mass of mixture A; the added amount of diacylglycerol lipase is 1.2% of the mass of mixture A; the added amount of monoacylglycerol lipase is 1.2% of the mass of mixture A; the added amount of papain is 3% of the mass of mixture A; the added amount of serrapeptase is 1% of the mass of mixture A; and the added amount of ficin is 1% of the mass of mixture A.

[0081] S2, Preparation of composite extract II:

[0082] According to the mass ratio, the ratio of Sanguinea spatholobi leaves: bamboo leaves: alfalfa leaves is 5:2.5:2. The Sanguinea spatholobi leaves, bamboo leaves and alfalfa leaves are mixed and crushed to obtain a mixture B. 12 times the mass of water is added to the mixture B, the pH is adjusted to 5.5, hemicellulase is added, and the mixture is hydrolyzed at 60°C for 3 hours. Then, the enzyme is inactivated in a water bath at 100°C for 40 minutes. After the mixture is cooled to 55°C, the pH is adjusted to 8.0, bromelain is added, and the mixture is hydrolyzed for 2 hours. The mixture is cooled to 37°C, the pH is adjusted to 2.2, pepsin is added, and the mixture is hydrolyzed for 1.5 hours. The mixture is inactivated in a water bath at 100°C for 40 minutes. After the mixture is cooled to room temperature, it is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, which is spray-dried to obtain a composite extract II.

[0083] The amount of hemicellulase added is 3% of the mass of the mixture B; the amount of bromelain added is 1.5% of the mass of the mixture B; and the amount of pepsin added is 1.5% of the mass of the mixture B.

[0084] S3, compounding of the composition:

[0085] In parts by mass, 1.5 parts of compound extract I, 1.5 parts of compound extract II, 1 part of hyaluronic acid, 0.8 parts of sorbitol, and 30 parts of purified water are mixed at 45° C. to obtain a composition for removing eye bags and lightening dark circles.

[0086] A composition preparation for removing eye bags and fading dark circles, the preparation is prepared from the following raw materials in parts by mass: 20 parts of the composition for removing eye bags and fading dark circles, 1 part of lecithin, 0.15 parts of sorbic acid, 0.8 parts of sodium alginate, 1.2 parts of carbomer, and 12 parts of purified water.

[0087] The above-mentioned composition preparation for removing eye bags and lightening dark circles is used for preparing cosmetics for removing eye bags and dark circles.

[0088] The preparation of the composition for removing eye bags and fading dark circles of Comparative Example 1 comprises the following steps:

[0089] S1. Preparation of composite extract I:

[0090] According to the mass ratio of kudzu kernel: papaya kernel=4:1.5, kudzu kernel and papaya kernel are mixed and crushed to obtain mixture A, 10 times the mass of water is added to mixture A, the pH is adjusted to 7.5, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and after enzymatic hydrolysis at 35°C for 4.5 hours, the temperature is raised to 55°C, the pH is adjusted to 6.0, papain is added, and enzymatic hydrolysis is continued for 2.5 hours, and then the enzyme is inactivated in a water bath at 90°C for 30 minutes, and then the temperature is lowered to 48°C, the pH is adjusted to 5.8, and serrapeptase is added for enzymatic hydrolysis for 1 hour, and the enzyme is inactivated in a water bath at 90°C for 30 minutes. After cooling to room temperature, the mixture is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, which is spray-dried to obtain composite extract I;

[0091] Among them, the added amount of triacylglycerol lipase is 0.8% of the mass of mixture A; the added amount of diacylglycerol lipase is 0.8% of the mass of mixture A; the added amount of monoacylglycerol lipase is 1% of the mass of mixture A; the added amount of papain is 2% of the mass of mixture A; and the added amount of serrapeptase is 0.8% of the mass of mixture A.

[0092] S2, Preparation of composite extract II:

[0093] According to the mass ratio, the leaves of Sanguinea sphaerocephala: the leaves of Bambusa longituba: the leaves of Alfalfa = 4:2:1, the leaves of Sanguinea sphaerocephala, the leaves of Bambusa longituba and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, 10 times the mass of water is added to the mixture B, the pH is adjusted to 5.0, hemicellulase is added, and enzymolysis is carried out at 55°C for 2.5 hours, and then the enzyme is inactivated in a water bath at 90°C for 30 minutes, and after the pH is reduced to 52°C, the pH is adjusted to 7.0, bromelain is added, and enzymolysis is carried out for 1.5 hours, and the enzyme is inactivated in a water bath at 90°C for 30 minutes, and after the pH is reduced to room temperature, it is filtered with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray-dried to obtain a composite extract II;

[0094] The amount of hemicellulase added is 2% of the mass of the mixture B; the amount of bromelain added is 1% of the mass of the mixture B.

[0095] S3, compounding of the composition:

[0096] In parts by mass, 1.2 parts of compound extract I, 1.2 parts of compound extract II, 0.6 parts of hyaluronic acid, 0.5 parts of sorbitol and 25 parts of purified water are mixed at 40° C. to obtain a composition for removing eye bags and lightening dark circles.

[0097] The composition preparation for removing eye bags and lightening dark circles of Comparative Example 1 is prepared from the following raw materials in parts by mass: 15 parts of the composition for removing eye bags and lightening dark circles, 0.5 parts of lecithin, 0.1 parts of sorbic acid, 0.5 parts of sodium alginate, 0.7 parts of carbomer, and 8 parts of purified water.

[0098] The preparation of the composition for removing eye bags and fading dark circles of comparative example 2 comprises the following steps:

[0099] S1. Preparation of composite extract I:

[0100] According to the mass ratio of kudzu kernel: papaya kernel=4:1.5, kudzu kernel and papaya kernel are mixed and crushed to obtain mixture A, 10 times the mass of water is added to mixture A, the pH is adjusted to 7.5, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and after enzymatic hydrolysis at 35°C for 4.5 hours, the temperature is raised to 55°C, the pH is adjusted to 6.0, papain is added, and enzymatic hydrolysis is continued for 2.5 hours, and then the enzyme is inactivated in a water bath at 90°C for 30 minutes, and after cooling to room temperature, a 2kDa ultrafiltration membrane is used for filtration to obtain a liquid below 2kDa, which is spray-dried to obtain composite extract I;

[0101] Among them, the added amount of triacylglycerol lipase is 0.8% of the mass of mixture A; the added amount of diacylglycerol lipase is 0.8% of the mass of mixture A; the added amount of monoacylglycerol lipase is 1% of the mass of mixture A; and the added amount of papain is 2% of the mass of mixture A.

[0102] S2, Preparation of composite extract II:

[0103] According to the mass ratio, the leaves of Sanguinea sphaerocephala: the leaves of Bambusa longituba: the leaves of Alfalfa = 4:2:1, the leaves of Sanguinea sphaerocephala, the leaves of Bambusa longituba and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, 10 times the mass of water is added to the mixture B, the pH is adjusted to 5.0, hemicellulase is added, and enzymolysis is carried out at 55°C for 2.5 hours, and then the enzyme is inactivated in a water bath at 90°C for 30 minutes, and after the pH is reduced to 52°C, the pH is adjusted to 7.0, bromelain is added, and enzymolysis is carried out for 1.5 hours, and the enzyme is inactivated in a water bath at 90°C for 30 minutes, and after the pH is reduced to room temperature, it is filtered with a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray-dried to obtain a composite extract II;

[0104] The amount of hemicellulase added is 2% of the mass of the mixture B; the amount of bromelain added is 1% of the mass of the mixture B.

[0105] S3, compounding of the composition:

[0106] In parts by mass, 1.2 parts of compound extract I, 1.2 parts of compound extract II, 0.6 parts of hyaluronic acid, 0.5 parts of sorbitol and 25 parts of purified water are mixed at 40° C. to obtain a composition for removing eye bags and lightening dark circles.

[0107] The composition preparation for removing eye bags and lightening dark circles of Comparative Example 2 is prepared from the following raw materials in parts by mass: 15 parts of the composition for removing eye bags and lightening dark circles, 0.5 parts of lecithin, 0.1 parts of sorbic acid, 0.5 parts of sodium alginate, 0.7 parts of carbomer, and 8 parts of purified water.

[0108] The preparation of the composition for removing eye bags and fading dark circles of comparative example 3 comprises the following steps:

[0109] S1 was omitted, and the composite extract I was not prepared; the composite extract I was not added to the compounding of the composition for removing eye bags and lightening dark circles; the other methods and parameters were the same as those in Example 1.

[0110] The preparation of the composition for removing eye bags and fading dark circles of Comparative Example 4 comprises the following steps:

[0111] S2 was omitted, and the composite extract II was not prepared; the composite extract II was not added to the compounding of the composition for removing eye bags and lightening dark circles; the other methods and parameters were the same as those in Example 1.

[0112] The above embodiments and comparative examples were tested.

[0113] 1. Skin patch test

[0114] 30 subjects (female aged 24 to 38) were selected, and 0.02 g of each sample of the composition for removing eye bags and lightening dark circles was placed in a spot tester chamber and applied to the inner arm of the subject. The spot tester was removed after 24 hours, and the skin condition was observed within 48 hours. No adverse reactions such as allergies, redness, swelling, itching, rash, and acne were found, and the results were safe.

[0115] 2. Heme oxygenase-1 gene expression test

[0116] The accumulation of heme in the dermis and epidermis will make the skin look purple and lead to the formation of dark circles around the eyes. Therefore, removing free heme can help reduce dark circles, and the increase of heme oxygenase-1 can catalyze the degradation of heme, achieving the effect of quickly removing dark circles. The higher the expression of heme oxygenase-1 gene, the more obvious the effect of removing dark circles.

[0117] Culture medium A (containing 5.5 μg / mL bovine insulin, 4.5 μg / mL bovine transferrin, 0.25% bovine pituitary hormone, 0.2 ng / mL epidermal growth factor, and 0.16 μg / mL cortisone) was added to a 6-well cell culture plate, and then keratinocytes were inoculated and incubated at 37°C with 5% CO. 2After 48 hours of culture under the conditions, the culture medium was replaced: the culture medium in the control group was replaced with fresh culture medium A, and the culture medium in the experimental group was replaced with fresh culture medium A containing 10wt% of the eye bag and dark circle removing composition (3 parallel samples for each), and the culture was continued for 24 hours; then washed with PBS solution, the total RNA in the cells was extracted, and the total RNA was used as a template, reverse transcribed into cDNA using reverse transcriptase, and then polymerase chain amplification was performed using cDNA as a template, and the gene expression of heme oxygenase-1 was detected using a specific probe of the heme oxygenase-1 gene. The expression of heme oxygenase-1 mRNA in the experimental group was calculated relative to that in the control group. The larger the value, the more the heme oxygenase-1 gene was expressed. Among them, the expression of the control group was normalized to 1 by the instrument, that is, the expression of the control group was 1. The calculation results are shown in Table 1 below:

[0118] Table 1 Heme oxygenase-1 gene relative expression test results (average value)

[0119]

[0120] The accumulation of heme in the dermis and epidermis will make the skin look purple and lead to the formation of dark circles around the eyes. Therefore, removing free heme can help reduce dark circles, and the increase of heme oxygenase-1 can catalyze the degradation of heme, achieving the effect of quickly removing dark circles. The higher the expression of heme oxygenase-1 gene, the more obvious the effect of removing dark circles.

[0121] From the above results, it can be seen that the eye bag removal and dark circle lightening compositions of Examples 1 to 5 have a good effect on promoting the expression of heme oxygenase-1. From Comparative Examples 1 and 2, it can be seen that the products obtained by different preparation methods have a greater impact on the degree of promoting the expression of heme oxygenase-1. The eye bag removal and dark circle lightening compositions of Comparative Examples 1 and 2 prepared after omitting the steps have a poor effect on promoting the expression of heme oxygenase-1. From Comparative Examples 3 and 4, it can be seen that compound extract I and compound extract II have a synergistic effect, which can greatly improve the effect. The effect of adding compound extract I or compound extract II alone is very low.

[0122] 3. Use effect verification experiment

[0123] 54 subjects (31-54 years old) were selected, half of them were male and half were female. All the subjects had no skin diseases and no history of skin allergies, but all the subjects had eye skin manifestations: dark circles and eye bags; they were divided into 9 groups, with 6 people in each group (3 males and 3 females). Each group used a corresponding preparation of the composition for removing eye bags and lightening dark circles, and applied it to the lower eyelids (eye bags) of both eyes after cleansing in the morning and evening, and massaged for absorption for 3 months.

[0124] (1) The I TA° value was tested before use and after 3 months of use; the VISA facial image analysis system and Image-Pro Plus image analysis software were used to test the I TA° value. The larger the I TA° value, the brighter the skin tone and the better the effect of removing dark circles. The test results are shown in Table 2 below:

[0125] Table 2I TA° value test results

[0126]

[0127] From the above results, it can be seen that the eye bag removal and dark circle lightening composition preparations of Examples 1 to 5 have a good effect of lightening dark circles. From Comparative Examples 1 and 2, it can be seen that different preparation methods have an impact on the degree of lightening dark circles, and the eye bag removal and dark circle lightening composition preparations of Comparative Examples 1 and 2 prepared by omitting steps have poor effects on promoting the lightening of dark circles. From Comparative Examples 3 and 4, it can be seen that compound extract I and compound extract II have a synergistic effect, which can greatly improve the effect of lightening dark circles.

[0128] (2) Test on the effect of alleviating eye bags

[0129] Primos was used to collect facial images for eye bag analysis; the eye bag volume change rate before and after use = (measured value after use - measured value before use) ÷ measured value before use × 100%; the test results are shown in Table 3 below:

[0130] Table 3 Numerical test results of eye bag volume

[0131]

[0132] From the above results, it can be seen that the combination of extracts of Rutaceae, papaya seeds, Sanguinea chinensis leaves, Bamboo leaves and Alfalfa leaves can effectively improve eye bags and reduce the volume of eye bags. Various extracts contain small molecule active substances and small molecule peptide substances, which can promote cell synthesis of collagen, restore skin elasticity, and improve cell metabolism and detoxification capabilities. Among them, the extracts of Sanguinea chinensis leaves, Bamboo leaves and Alfalfa leaves have the ability to promote blood circulation and reduce swelling, and have a good effect on removing eye bags; the extracts of Rutaceae, papaya seeds, Bamboo leaves and Alfalfa leaves have good antioxidant and anti-aging effects, reduce wrinkles, lift the skin, and thus delay the development of eye bags.

Claims

1. A method for preparing a composition for removing eye bags and lightening dark circles, characterized in that: The steps include: S1. Preparation of composite extract I: According to the mass ratio, the kernel of the safflower seed: the kernel of papaya seed = (3-5): (1-2), the kernel of the safflower seed and the kernel of papaya seed are mixed and crushed to obtain a mixture A, water is added to the mixture A, the pH is adjusted to 7.2-7.8, triacylglycerol lipase, diacylglycerol lipase and monoacylglycerol lipase are added, and the mixture is enzymatically hydrolyzed at 32-38°C for 4-5h, the temperature is raised to 50-60°C, the pH is adjusted to 5.0-7.0, papain is added, and the enzymatic hydrolysis is continued for 2-3h, and then the enzyme is inactivated in a water bath, the temperature is lowered to 45-50°C, the pH is adjusted to 5.5-6.0, serrapeptase is added for enzymatic hydrolysis for 1h-1.5h, the temperature is raised to 60-65°C, ficin is added, the enzymatic hydrolysis is continued for 1h-1.5h, the enzyme is inactivated in a water bath, the temperature is lowered to room temperature, and a 2kDa ultrafiltration membrane is used to obtain a liquid below 2kDa, which is spray-dried to obtain a composite extract I; S2, Preparation of composite extract II: According to the mass ratio, the leaves of Sanguinea sphaerocephala: the leaves of Psoralea corylifolia: the leaves of Alfalfa = (3-5): (1-2.5): (0.5-2), the leaves of Sanguinea sphaerocephala, the leaves of Psoralea corylifolia and the leaves of Alfalfa are mixed and crushed to obtain a mixture B, water is added to the mixture B, the pH is adjusted to 4.0-5.5, hemicellulase is added, and the mixture is hydrolyzed at 50-60°C for 2h-3h, and then the enzyme is inactivated by water bath, and after the mixture is cooled to 50-55°C, the pH is adjusted to 6.0-8.0, bromelain is added, and the mixture is hydrolyzed for 1.5h-2h, and the temperature is cooled to 35-37°C, and the pH is adjusted to 1.8-2.2, and pepsin is added, and the mixture is hydrolyzed for 1h-1.5h, and the enzyme is inactivated by water bath, and after the mixture is cooled to room temperature, it is filtered using a 2kDa ultrafiltration membrane to obtain a liquid below 2kDa, and spray dried to obtain a composite extract II; S3, compounding of the composition: 1 to 1.5 parts of compound extract I, 1 to 1.5 parts of compound extract II, 0.5 to 1 part of hyaluronic acid, 0.3 to 0.8 parts of sorbitol and 20 to 30 parts of purified water are mixed in parts by mass to obtain a composition for removing eye bags and lightening dark circles.

2. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S1, the amount of water added is 8 to 12 times the mass of the mixture A.

3. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S1, the added amount of the triacylglycerol lipase is 0.5% to 1.2% of the mass of the mixture A; the added amount of the diacylglycerol lipase is 0.5% to 1.2% of the mass of the mixture A; and the added amount of the monoacylglycerol lipase is 0.5% to 1.2% of the mass of the mixture A.

4. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S1, the amount of papain added is 1% to 3% of the mass of the mixture A; the amount of serrapeptase added is 0.5% to 1% of the mass of the mixture A; and the amount of ficin added is 0.5% to 1% of the mass of the mixture A.

5. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S2, the amount of water added is 8 to 12 times the mass of the mixture B.

6. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S2, the amount of hemicellulase added is 1% to 3% of the mass of mixture B; the amount of bromelain added is 0.5% to 1.5% of the mass of mixture B; and the amount of pepsin added is 0.5% to 1.5% of the mass of mixture B.

7. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S1 and S2, the temperature of the water bath inactivation of enzyme is 85°C to 100°C, and the time is 25min to 40min.

8. The method for preparing a composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: In S3, the mixing temperature is 30°C to 45°C.

9. A composition preparation for removing eye bags and lightening dark circles, comprising the composition for removing eye bags and lightening dark circles prepared by the preparation method of the composition for removing eye bags and lightening dark circles according to claim 1, characterized in that: The preparation is prepared from the following raw materials in parts by mass: 10 to 20 parts of a composition for removing eye bags and lightening dark circles, 0.3 to 1 part of lecithin, 0.05 to 0.15 parts of sorbic acid, 0.3 to 0.8 parts of sodium alginate, 0.5 to 1.2 parts of carbomer and 6 to 12 parts of purified water.

10. Use of the composition for removing eye bags and lightening dark circles according to claim 9 in preparing cosmetics for removing eye bags and / or dark circles.

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