A Chinese medicine composition having the effect of enhancing immune function and its preparation method

Abstract

The invention discloses a Chinese medicine composition with the function of enhancing immune function and a preparation method thereof; the Chinese medicine composition is composed of one or more of parts A to F; wherein the weight proportions of the medicinal materials in parts A to F are as follows: part A comprises 1 to 50 parts of astragalus, 1 to 50 parts of ginseng, and 1 to 50 parts of codonopsis; part B comprises 1 to 40 parts of angelica, 1 to 40 parts of jujube, and 1 to 40 parts of longan pulp; part C comprises 1 to 30 parts of cistanche, 1 to 30 parts of eucommia leaf, 1 to 30 parts of alpinia oxyphylla, and 1 to 30 parts of ginseng seed. ~30 parts; Part D includes 1~20 parts of lily, 1~20 parts of polygonatum, 1~20 parts of wolfberry, and 1~20 parts of mulberry; Part E includes 1~20 parts of cornus and 1~20 parts of raspberry; Part F includes 1~10 parts of tuckahoe, 1~10 parts of yam, 1~10 parts of hawthorn, and 1~10 parts of tangerine peel; the Chinese medicine composition can be made into solid beverages, compressed candies, pastes, honey pills, functional jellies, biscuits, etc. in food after extraction or crushing and adding appropriate pharmaceutical excipients. The Chinese medicine composition has strong effects of strengthening the body, strengthening the spleen and lungs, and replenishing qi and blood, can effectively enhance immunity and improve physical fitness, and provides patients with a safe and effective pure Chinese medicine rehabilitation food.

Description

Technical Field

[0001] The present invention relates to the field of traditional Chinese medicine and food, and in particular to a traditional Chinese medicine composition having the effect of enhancing immune function and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine believes that the human body is an organic whole, "when yin and yang are balanced, the spirit is healthy". In a healthy state, the human body maintains a normal physiological state of yin and yang balance and qi and blood circulation. When the body is invaded by external evil, and the immune system is not enough to identify and eliminate the "foreign" physiological reaction, it will cause the body to become ill or be in a sub-healthy state. As mentioned in "Huangdi Neijing Suwen": "When the positive energy is stored, the evil cannot interfere; where the evil gathers, the energy must be weak". With the accelerated pace of life in modern society, the arrival of the aging population, and some physical and mental health problems caused by busy work, the immunity of modern people is getting weaker and weaker, and many sub-health problems have emerged, such as: physical weakness, mental depression, fatigue and weakness, etc. According to statistics from the World Health Organization, about 35% to 50% of people in the world are in a "sub-healthy" state with fatigue, decreased vitality and reduced immunity as the main clinical manifestations [Xin Liyuan, Zheng Liang. Study on the mechanism of action and medication rules of Chinese medicine immunoenhancer based on data mining and network pharmacology. Journal of Nanjing University of Chinese Medicine, 2024, 40(2), 174-183.] These symptoms are present in almost all adults to varying degrees, especially in the middle-aged and elderly. Therefore, enhancing immunity and improving the body's resistance to pathogens has become a major issue that people are paying more and more attention to today. Strengthening the body's health and eliminating pathogens is the core principle of TCM to improve immunity. Strengthening the body's health refers to supporting the body's positive energy, strengthening the body's constitution, and improving the body's resistance to disease. By strengthening the body's positive energy, the pathogens can be eliminated and the health status can be restored or maintained.

[0003] Traditional Chinese medicine is derived from practice, and has a clear role in preventing, curing and maintaining health. The earliest existing medical book in my country, Shennong's Herbal Classic, contains 365 kinds of medicines, which are divided into three categories according to the upper, middle and lower grades, including 120 upper-grade, 120 middle-grade and 125 lower-grade. Among them, the upper-grade medicine nourishes life and responds to the will of heaven, and is non-toxic. Those who want to lighten the body, invigorate the qi, and prolong life can take it for a long time. It can be seen that there has been a theory of "medicine and food have the same origin" since ancient times. After continuous development, a relatively complete disease prevention, treatment and health care system has been formed. Among the "list of items that are both food and medicine" announced by the former Ministry of Health in 2002, 87 of them are all derived from Chinese medicinal materials and are widely used in traditional Chinese medicine. After continuous supplementation, by 2023, there are more than 100 kinds of Chinese medicine that can be used as food.

[0004] Based on the catalogue of medicines and foods with the same origin published by the National Health Commission and ancient classic prescriptions, a Chinese medicine composition with the functions of replenishing qi and blood and enhancing immunity has been developed through scientific formulation, and then made into food, which is of great significance for improving sub-health conditions. Summary of the invention

[0005] The purpose of the present invention is to provide a Chinese medicine composition having the effect of enhancing immune function and a preparation method thereof, so as to overcome the deficiencies in the prior art.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The present application discloses a method for preparing a Chinese medicine composition having the effect of enhancing immune function, characterized in that: the Chinese medicine composition is composed of one or more of parts A to F;

[0008] The weight proportions of the medicinal materials in parts A to F are as follows: part A includes 1 to 50 parts of astragalus, 1 to 50 parts of ginseng, and 1 to 50 parts of codonopsis; part B includes 1 to 40 parts of angelica, 1 to 40 parts of jujube, and 1 to 40 parts of longan pulp; part C includes 1 to 30 parts of cistanche, 1 to 30 parts of eucommia leaves, and 1 to 30 parts of alpinia oxyphylla; part D includes 1 to 20 parts of lily, 1 to 20 parts of polygonatum, 1 to 20 parts of wolfberry, and 1 to 20 parts of mulberry; part E includes 1 to 20 parts of cornus officinalis and 1 to 20 parts of raspberry; part F includes 1 to 10 parts of tuckahoe, 1 to 10 parts of yam, 1 to 10 parts of hawthorn, and 1 to 10 parts of dried tangerine peel;

[0009] The preparation method is as follows:

[0010] S1. Select one or more combinations required in parts A to F; mix the corresponding medicinal materials;

[0011] S2, extracting the mixed medicinal materials with water 6 to 60 times the weight of the mixed medicinal materials 2 to 3 times, each time for 20 to 120 minutes to obtain the corresponding extract;

[0012] S3, combining the two extracts, concentrating under reduced pressure, and obtaining the Chinese medicine composition.

[0013] Preferably, in step S2, the extract is obtained by heating extraction or ultrasonic extraction, the temperature of the heating extraction is maintained at 50-100°C, and the temperature of the ultrasonic extraction is maintained at 20-70°C.

[0014] Preferably, in step S3, the combined extract is concentrated under reduced pressure to an extract having a weight ratio of 1:0.5 to 1:3 to the medicinal material, thereby obtaining the Chinese medicine composition; or the combined extract is concentrated under reduced pressure to a solution having a relative density of 1.05 to 1.20, and the Chinese medicine composition is obtained after spray drying.

[0015] Preferably, the temperature range of the reduced pressure concentration is 50-70°C.

[0016] Preferably, in step S2, mulberries, wolfberries, longan pulp and raspberries are extracted by squeezing fresh fruit juice and then spray drying.

[0017] The invention also discloses a Chinese medicine composition with the effect of enhancing immune function, which is prepared by the preparation method of the Chinese medicine composition with the effect of enhancing immune function as described above; the Chinese medicine composition is composed of one or more of parts A to F; wherein the weight parts of each medicinal material in parts A to F are as follows: part A includes 1 to 50 parts of astragalus, 1 to 50 parts of ginseng, and 1 to 50 parts of codonopsis; part B includes 1 to 40 parts of angelica, 1 to 40 parts of jujube, and 1 to 40 parts of longan pulp; part C includes 1 to 30 parts of cistanche, 1 to 30 parts of eucommia leaves, and 1 to 30 parts of alpinia oxyphylla; part D includes 1 to 20 parts of lily, 1 to 20 parts of polygonatum, 1 to 20 parts of wolfberry, and 1 to 20 parts of mulberry; part E includes 1 to 20 parts of cornus officinalis and 1 to 20 parts of raspberry; part F includes 1 to 10 parts of tuckahoe, 1 to 10 parts of yam, 1 to 10 parts of hawthorn, and 1 to 10 parts of tangerine peel.

[0018] Preferably, the weight proportions of the medicinal materials in parts A to F are as follows: part A includes 10-40 parts of astragalus, 10-40 parts of ginseng, and 10-40 parts of codonopsis; part B includes 10-30 parts of angelica, 10-30 parts of jujube, and 10-30 parts of longan pulp; part C includes 10-20 parts of cistanche, 10-20 parts of eucommia leaves, and 10-20 parts of alpinia oxyphylla; part D includes 5-15 parts of lily, 5-15 parts of polygonatum, 5-15 parts of wolfberry, and 5-15 parts of mulberry; part E includes 5-15 parts of cornus officinalis and 5-15 parts of raspberry; part F includes 3-9 parts of tuckahoe, 3-9 parts of yam, 3-9 parts of hawthorn, and 3-9 parts of tangerine peel.

[0019] Preferably, the weight proportions of the medicinal materials in parts A to F are as follows: part A includes 20-30 parts of astragalus, 20-30 parts of ginseng, and 20-30 parts of codonopsis; part B includes 10-20 parts of angelica, 10-20 parts of jujube, and 10-20 parts of longan pulp; part C includes 10-15 parts of cistanche, 10-15 parts of eucommia leaves, and 10-15 parts of alpinia oxyphylla; part D includes 5-10 parts of lily, 5-10 parts of polygonatum, 5-10 parts of wolfberry, and 5-10 parts of mulberry; part E includes 6-12 parts of cornus officinalis and 6-12 parts of raspberry; part F includes 4-8 parts of tuckahoe, 4-8 parts of yam, 4-8 parts of hawthorn, and 4-8 parts of tangerine peel.

[0020] Preferably, the Chinese medicine composition is used in the preparation of immunity enhancing food.

[0021] Preferably, the food forms prepared from the Chinese medicine composition include solid beverages, compressed candies, pastes, honey pills, functional jellies, and biscuits.

[0022] Beneficial effects of the present invention:

[0023] Compared with the prior art, the present invention has the following beneficial effects: the present invention provides a new Chinese medicine composition with the functions of replenishing qi and blood and enhancing immune function and a preparation method thereof. The composition can effectively improve symptoms such as mental fatigue, fatigue, loss of appetite, and sleep disorders caused by low immune function. At the same time, since it is made into food from Chinese medicine that is both medicinal and edible, it provides a safer and long-term solution for improving immunity compared to chemical drugs and other Chinese medicines.

[0024] The product of the present invention has been proved by animal pharmacology experiments to have significant effects in enhancing immunity and resisting fatigue. Studies have shown that the new formula composed of six parts of AF has better animal efficacy than any single formula of AF and the compound formula of AD.

[0025] The features and advantages of the present invention will be described in detail through embodiments. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below through embodiments. However, it should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the scope of the present invention. In addition, in the following description, the description of known structures and technologies is omitted to avoid unnecessary confusion of the concept of the present invention.

[0027] The embodiment of the present invention provides a method for preparing a Chinese medicine composition having the effect of enhancing immune function, wherein the Chinese medicine composition is composed of one or more of parts A to F;

[0028] The weight proportions of the medicinal materials in parts A to F are as follows: part A includes 1 to 50 parts of astragalus, 1 to 50 parts of ginseng, and 1 to 50 parts of codonopsis; part B includes 1 to 40 parts of angelica, 1 to 40 parts of jujube, and 1 to 40 parts of longan pulp; part C includes 1 to 30 parts of cistanche, 1 to 30 parts of eucommia leaves, and 1 to 30 parts of alpinia oxyphylla; part D includes 1 to 20 parts of lily, 1 to 20 parts of polygonatum, 1 to 20 parts of wolfberry, and 1 to 20 parts of mulberry; part E includes 1 to 20 parts of cornus officinalis and 1 to 20 parts of raspberry; part F includes 1 to 10 parts of tuckahoe, 1 to 10 parts of yam, 1 to 10 parts of hawthorn, and 1 to 10 parts of dried tangerine peel;

[0029] The preparation method is as follows:

[0030] S1. Select one or more combinations required in parts A to F; mix the corresponding medicinal materials;

[0031] S2, extracting the mixed medicinal materials with water 6 to 60 times the weight of the mixed medicinal materials 2 to 3 times, each time for 20 to 120 minutes to obtain the corresponding extract;

[0032] S3, combining the two extracts, concentrating under reduced pressure, and obtaining the Chinese medicine composition.

[0033] In a feasible embodiment, in step S2, the extract is obtained by heating extraction or ultrasonic extraction, the temperature of the heating extraction is maintained at 50-100°C, and the temperature of the ultrasonic extraction is maintained at 20-70°C.

[0034] In a feasible embodiment, in step S3, the combined extract is concentrated under reduced pressure to an extract having a weight ratio of 1:0.5 to 1:3 to the medicinal material, thereby obtaining the Chinese medicine composition; or the combined extract is concentrated under reduced pressure to a solution having a relative density of 1.05 to 1.20, and the Chinese medicine composition is obtained after spray drying.

[0035] In a feasible embodiment, in step S2, mulberries, wolfberries, longan pulp and raspberries are extracted by squeezing fresh fruit juice and then spray drying.

[0036] A Chinese medicine composition having an effect of enhancing immune function, prepared by the method for preparing a Chinese medicine composition having an effect of enhancing immune function as claimed in any one of claims 1 to 5;

[0037] The food forms prepared by the Chinese medicine composition include solid beverages, compressed candies, pastes, honey pills, functional jellies, and biscuits:

[0038] In some specific embodiments, the method for preparing the solid beverage in the form of food is to mix the extract or raw material with an appropriate amount of food auxiliary materials to make a soft material, and then sieve it through a 16-mesh sieve to make particles, and then dry it to obtain a solid beverage.

[0039] In some specific embodiments, the method for preparing the compressed candy in the form of the above-mentioned food is to mix the above-mentioned extract or raw material with an appropriate amount of food auxiliary material, slowly spray a certain concentration of ethanol aqueous solution on its surface under stirring to make a soft material, and then granulate, dry, granulate, and compress the material to obtain the compressed candy after drying.

[0040] In some specific embodiments, the method for preparing the above-mentioned food-form paste is to concentrate the above-mentioned traditional Chinese medicine extract until the medicine juice drips in a linear shape after stirring, and then add auxiliary materials such as gum and honey, boil and refine repeatedly, and stir evenly until the boiled paste is pulled into threads.

[0041] In some specific embodiments, the preparation method of the honey pills in the form of food is as follows: all the weight portions of the Chinese medicine are weighed and then mixed and powdered, or all the weight portions of the Chinese medicine are weighed and then mixed and extracted, the extract is spray-dried to obtain powder, honey is prepared according to the ratio of powder to honey = 1:0.5 to 1:1.5, the prepared honey is refined, the refined honey is slowly added to the powder with a spoon while stirring, and the addition of honey is stopped when the dry powder is basically invisible, the honey and powder are mixed into a ball, a small amount of sesame oil is applied to the palm, the ball of soft medicine material is rubbed, first divided into lumps and rolled into strips, then cut into sections, each section is rolled into a pill, and after the pills sweat and dry and the surface becomes hard, they are packaged in wax shells.

[0042] In some specific embodiments, the preparation method of the functional jelly in the form of food is as follows: all the weight portions of the Chinese medicine are weighed and then mixed and extracted, the extract is concentrated to a certain volume, excipients such as glue are taken and mixed, a small amount of pure water is added, and the mixture is allowed to stand for a period of time to fully swell, and then the gel is heated and boiled until the composite gel is heated to have no particles, the boiled mixed gel is added to the Chinese medicine extract while it is hot, a flavoring agent is added and mixed evenly, the mixture is quickly filtered, and the mixture is canned into a jelly cup, and the jelly is sterilized in an autoclave after sealing, and the gel is taken out and cooled to room temperature to obtain a finished jelly.

[0043] In some specific embodiments, the method for preparing the above-mentioned food-form biscuits is as follows: weigh a certain amount of Chinese medicine extract raw material, add appropriate amount of water in a beater to make a slurry, add xanthan gum, flour, baking soda, sugar, salt, etc. to make dough, let it rise at room temperature for a certain time, use a dough press to press the dough into a certain thickness, use a mold to make a dough blank, and pierce holes, put the pierced dough blank into a baking tray, place it in a preheated oven, bake it in an oven with an upper fire temperature of 180°C and a bottom fire temperature of 160°C for a certain time, and obtain it after cooling at room temperature.

[0044] Embodiment 1:

[0045] The preparation method of the Chinese medicine composition QFP of the present invention comprises the following steps: 1000g of astragalus, 1000g of ginseng, 1000g of codonopsis, 800g of angelica, 800g of jujube, 800g of longan pulp (fresh fruit), 600g of cistanche deserticola, 600g of eucommia leaf, 600g of alpinia oxyphylla, 400g of lily, 400g of polygonatum, 400g of wolfberry (fresh fruit), 400g of mulberry (fresh fruit), 400g of cornus officinalis, 400g of raspberry (fresh fruit), 200g of tuckahoe, 200g of yam, 200g of hawthorn and 200g of tangerine peel. The above drugs, mulberry, wolfberry, longan pulp and raspberry juice are squeezed, and the remaining drugs are combined, and extracted twice with 100L and 80L of water respectively, each time for 1 hour, and the filtrates are combined after filtration, and then combined with the squeezed juice, and concentrated under reduced pressure at 65°C to 10kg of paste, or concentrated under reduced pressure at 65°C to a solution with a relative density of 1.05 (measured at 40°C), and spray-dried to obtain the whole formula raw material, numbered QFP.

[0046] Embodiment 2:

[0047] Preparation of the Chinese medicine composition AP in Part A of the present invention: 2000g of Astragalus, 2000g of Ginseng, and 2000g of Codonopsis. The above drugs are combined, heated to 50°C with 60L and 48L of water, respectively, and ultrasonically extracted twice, each time for 1 hour, filtered and combined with the filtrate, concentrated under reduced pressure at 50°C to 4kg of paste, or concentrated under reduced pressure at 50°C to a solution with a relative density of 1.10 (measured at 40°C), spray-dried to obtain the raw material of Part A, numbered AP.

[0048] Embodiment 3:

[0049] Preparation of the Chinese medicine composition BP of Part B of the present invention: 2000g of Chinese angelica, 2000g of jujube, and 2000g of longan pulp. The above drugs are combined, and extracted twice with 60L and 48L of water heated to 80°C, each time for 1 hour, and the filtrates are combined after filtration, and concentrated under reduced pressure at 50°C to 15kg of paste, or concentrated under reduced pressure at 50°C to a solution with a relative density of 1.15 (measured at 40°C), and spray-dried to obtain the raw material of Part B, numbered BP.

[0050] Embodiment 4:

[0051] Preparation of the Chinese medicine composition CP of part C of the present invention: 2000g of Cistanche deserticola, 2000g of Eucommia ulmoides leaves, and 2000g of Alpinia oxyphylla. The above drugs are combined, heated and boiled with 60L and 48L of water for extraction twice, each time for 1 hour, filtered and combined with the filtrate, concentrated under reduced pressure at 75°C to 12kg of paste, or concentrated under reduced pressure at 50°C to a solution with a relative density of 1.20 (measured at 40°C), spray-dried to obtain the raw material of part C, numbered CP.

[0052] Embodiment 5:

[0053] Preparation of the Chinese medicine composition DP of part D of the present invention: 2000g of lily, 2000g of polygonatum, 2000g of wolfberry, and 2000g of mulberry. The above drugs are combined, heated and boiled with 80L and 64L of water for extraction twice, each time for 1 hour, filtered and combined with the filtrate, concentrated under reduced pressure at 90°C to 8kg of paste, or concentrated under reduced pressure at 70°C to a solution with a relative density of 1.16 (measured at 40°C), spray-dried to obtain the raw material of part D, numbered DP.

[0054] Embodiment 6:

[0055] Preparation of the Chinese medicine composition EP in part E of the present invention: 3000 g of Cornus officinalis and 3000 g of Raspberry. The two herbs are combined, heated and boiled with 60 L and 48 L of water for extraction twice, each time for 1 hour, filtered and combined with the filtrate, concentrated under reduced pressure at 60° C. to 5 kg of paste, or concentrated under reduced pressure at 60° C. to a solution with a relative density of 1.08 (measured at 40° C.), spray dried to obtain the raw material of part E, numbered EP.

[0056] Embodiment 7:

[0057] Preparation of the Chinese medicine composition FP in part F of the present invention: 2000g of Poria cocos, 2000g of Chinese yam, 2000g of hawthorn, and 2000g of dried orange peel. The above drugs are combined, heated and boiled with 80L and 64L of water for extraction twice, each time for 1 hour, filtered and combined with the filtrate, concentrated under reduced pressure at 65°C to 18kg of paste, or concentrated under reduced pressure at 60°C to a solution with a relative density of 1.18 (measured at 40°C), spray-dried to obtain the raw material of part F, numbered FP.

[0058] Embodiment 8:

[0059] Preparation of AD Chinese medicine composite composition ADP of the present invention: 1000g of Astragalus, 1000g of Ginseng, 1000g of Codonopsis, 800g of Angelica, 800g of Jujube, 800g of Longan pulp, 600g of Cistanche, 600g of Eucommia leaf, 600g of Alpinia oxyphylla, 400g of Lilium, 400g of Polygonatum, 400g of Lycium barbarum, 400g of Mulberry. The above drugs are combined, heated and boiled with 88L and 70L of water for extraction twice, each time for 1 hour, filtered and combined with the filtrate, concentrated under reduced pressure at 65°C to 20kg of paste, or concentrated under reduced pressure at 60°C to a solution with a relative density of 1.20 (measured at 40°C), spray dried to obtain AD raw material, numbered ADP.

[0060] Embodiment 9:

[0061] Preparation of solid beverage: Take 100g of the extract of Example 1, add 150g of maltodextrin, 50g of white sugar, and 10g of citric acid respectively, mix and put into a blender and stir for 30 minutes to mix the materials evenly, granulate with a 10-mesh sieve, dry below 70°C, granulate, and pack in 6g bags to obtain a solid beverage.

[0062] Embodiment 10:

[0063] Preparation of tablet candy: Take 100g of the powder of Example 2, mix it evenly with 25g of maltodextrin, 25g of oligoxylose, 1g of stevioside, and 2g of citric acid, and slowly spray a certain concentration of ethanol aqueous solution on its surface under stirring to make a soft material. Put the prepared soft material into a swing granulator (20 mesh sieve) to obtain wet granules. Put the wet granules into a drying oven for drying, and the drying temperature is maintained at 65°C. After drying, granulate them, add a certain mass ratio of magnesium stearate as a lubricant to the obtained granules, adjust the appropriate pressure and tablet weight, prepare tablets with a tablet weight of 1g, and obtain the finished product after packaging.

[0064] Embodiment 11:

[0065] Preparation of the paste: Take 100g of the thick paste of Example 3, add an appropriate amount of honey to the thick paste-like liquid medicine, heat it with a low heat, and slowly stir the liquid medicine until dense bubbles continue to appear. At this time, use a thin stick to pick up the paste. If the paste falls off in pieces, stop heating, put it into a can, and cool it to room temperature after about 24 hours.

[0066] Embodiment 12:

[0067] Preparation of honey pills: Take an appropriate amount of honey, heat and refine at 120°C until more reddish-brown bubbles appear in the honey and it is shiny. At this time, the viscosity of the refined honey is large, and white silk can be pulled out. Cool to 60°C for standby use. Take 100g of the powder of Example 4, slowly add the refined honey to the powder with a spoon, stir continuously, mix the refined honey and the powder evenly, and then form a mass. Take a small amount of sesame oil and apply it to the palm, rub the above-mentioned mass food soft material, first divide it into 30 pieces, rub it into strips, cut each strip into 10 sections, and rub each section into a pill. When the pills sweat, after drying and hardening the appearance, package them with wax shells and store them in a cool and dry place.

[0068] Embodiment 13:

[0069] Preparation of functional jelly: Take about 100g of the thick paste of Example 5. First, mix carrageenan and xanthan gum in a ratio of 1:1, add a certain amount of water, heat and dissolve completely, add appropriate amount of milk powder and white sugar to dissolve, add to the thick paste, continue heating and stir evenly. Cool to 65°C, adjust the colloidal pH to 6.0 with citric acid and sodium citrate, add 0.1% KCl, mix evenly, fill while hot, sterilize at 110°C for 15 minutes, and cool at room temperature to form.

[0070] Embodiment 14:

[0071] Preparation of biscuits: Take about 100g of the thick paste of Example 6, add 500g of low-gluten flour, 60g of wheat starch, and 80g of milk powder for premixing, dissolve 5g of salt and 10g of composite leavening agent in 200ml of water, mix with 100g of vegetable oil, and add to the mixed powder in 3 times, mix and knead until the dough surface is smooth and the texture is soft, then seal the dough and let it stand in a proofing box at 35°C for 15min. The dough after standing is rolled into a cake embryo with uniform thickness (about 3.5mm), smooth surface and flat shape with a dough press. Spread the cake embryo flat on a baking tray and put it in the oven, set the upper and lower fires to 165°C, bake for 15min, and cool naturally to room temperature.

[0072] Immunity test - Effect on NK cell activity:

[0073] Test method: Take the raw materials of Examples 1-8, take 250 healthy mice, and randomly divide them into 25 groups, with 10 mice in each group: (1) blank control group; (2) QFP low-dose group 100 mg / kg; (3) QFP medium-dose group 200 mg / kg; (4) QFP high-dose group 400 mg / kg; (5) AP low-dose group 100 mg / kg; (6) AP medium-dose group 200 mg / kg; (7) AP high-dose group 400 mg / kg; (8) BP low-dose group 100 mg / kg; (9) BP medium-dose group 200 mg / kg; (10) BP high-dose group 400 mg / kg; (11) CP low-dose group 100 mg / kg; (12) CP medium-dose group 200 mg / kg g; (13) CP high-dose group 400 mg / kg; (14) DP low-dose group 100 mg / kg; (15) DP medium-dose group 200 mg / kg; (16) DP high-dose group 400 mg / kg; (17) EP low-dose group 100 mg / kg; (18) EP medium-dose group 200 mg / kg; (19) EP high-dose group 400 mg / kg; (20) FP low-dose group 100 mg / kg; (21) FP medium-dose group 200 mg / kg; (22) FP high-dose group 400 mg / kg; (23) ADP low-dose group 100 mg / kg; (24) ADP medium-dose group 200 mg / kg; (25) ADP high-dose group 400 mg / kg. The drug was administered by gavage, and the dosage was adjusted according to the weekly weight changes of the animals. The mice were killed 30 days after administration, and the spleen was removed under sterile conditions. The removed spleen was ground and passed through a 200-mesh sieve to prepare a single-cell suspension, and the cell concentration was adjusted with complete culture medium. 100 μL of target cells and spleen lymphocytes were added to a 96-well plate, respectively. After the process was completed, the optical density (OD) at 490 nm was measured using an ELISA reader and the NK cell activity was calculated. The results are shown in Table 1.

[0074] Table 1 Effects of various compositions on mouse NK cell activity

[0075]

[0076]

[0077] Note: Compared with the control group *P<0.05; **P<0.01; P<0.001.

[0078] As can be seen from Table 1, the NK cell activity of the low, medium and high dose groups of QFP was significantly higher than that of the control group, and this difference was statistically significant (*P<0.05; **P<0.01; P<0.001), and was dose-dependent. The remaining A, B, C, D, E and ADP compositions also had certain activity, but they were weaker than the QFP combination at the same dose. This result shows that the above-mentioned partial compositions all had a positive effect on the NK cell activity of mice, and they had the effect of enhancing the activity of NK cells, but the composition composed of the six parts of AF had a synergistic immune enhancement effect, which was better than other compositions.

[0079] Immunity test - mouse carbon clearance experiment:

[0080] Test method: Take the raw materials of Examples 1-8, take 250 healthy mice, and randomly divide them into 25 groups, with 10 mice in each group: (1) blank control group; (2) QFP low-dose group 100 mg / kg; (3) QFP medium-dose group 200 mg / kg; (4) QFP high-dose group 400 mg / kg; (5) AP low-dose group 100 mg / kg; (6) AP medium-dose group 200 mg / kg; (7) AP high-dose group 400 mg / kg; (8) BP low-dose group 100 mg / kg; (9) BP medium-dose group 200 mg / kg; (10) BP high-dose group 400 mg / kg; (11) CP low-dose group 100 mg / kg; (12) CP medium-dose group 200 mg / kg g; (13) CP high-dose group 400 mg / kg; (14) DP low-dose group 100 mg / kg; (15) DP medium-dose group 200 mg / kg; (16) DP high-dose group 400 mg / kg; (17) EP low-dose group 100 mg / kg; (18) EP medium-dose group 200 mg / kg; (19) EP high-dose group 400 mg / kg; (20) FP low-dose group 100 mg / kg; (21) FP medium-dose group 200 mg / kg; (22) FP high-dose group 400 mg / kg; (23) ADP low-dose group 100 mg / kg; (24) ADP medium-dose group 200 mg / kg; (25) ADP high-dose group 400 mg / kg. The drugs were administered by gavage, and the dosage was adjusted according to the weekly changes in the body weight of the animals. The duration of the entire experiment was 14 days. 30 minutes after the last administration, each mouse was injected with India ink at a rate of 0.1 ml / 10 g body weight through the tail vein. 20 μl of blood was collected from the mouse orbital vein at 1 minute and 5 minutes respectively, added to 2 ml of 0.1% NaCO3 solution and shaken well. The optical density (OD) was then measured colorimetrically at 680 nm using a spectrophotometer, and the clearance index (K) was calculated. The results are shown in Table 2.

[0081] Table 2 Effects of various compositions on carbon particle clearance index in mice

[0082]

[0083] Note: Compared with the control group *P<0.05; **P<0.01; P<0.001.

[0084] As can be seen from Table 2, the carbon clearance index of mice in the model group decreased significantly. The carbon clearance index of the low, medium and high dose groups of QFP increased significantly, and the difference was statistically significant (*P<0.05; **P<0.01; P<0.001), and was dose-dependent. The remaining A, B, C, D, E and ADP compositions also had certain activity, but they were weaker than the QFP combination at the same dose. This result shows that some of the above-mentioned compositions have a positive effect on the carbon clearance and phagocytosis function of mice, but the composition composed of the six parts of AF has a synergistic immune enhancement effect, which is better than other compositions.

[0085] Immunity Enhancement—Clinical Trial:

[0086] Experimental method: Take the raw materials of Examples 1, 2, 3, 4, and 8, and make solid beverages according to the method of Example 1, numbering QFP sample, AP sample, BP sample, CP sample, and ADP sample. Randomly select 90 people who are prone to fatigue, pale complexion, low immunity, and prone to colds, and investigate the improvement of their physical condition after use by questionnaire. The inclusion criteria are subjects who meet the conditions after physical examination and have not taken food or medicine related to the tested function recently. Randomly divide into groups, with 15 cases in each group, namely blank group (not taking any medicine or health care products); QFP sample group; AP sample group; BP sample group; CP sample group and ADP sample group. The age of the blank group was 35-70 years old, with an average age of 51 years old; the age of the QFP group was 36-71 years old, with an average age of 51 years old; the age of the AP group was 33-70 years old, with an average age of 51 years old; the age of the BP group was 34-69 years old, with an average age of 51 years old; the age of the CP group was 36-69 years old, with an average age of 51 years old; the age of the ADP group was 35-68 years old, with an average age of 51 years old. There was no statistically significant difference in the general data of the six groups, such as gender, age, and physical signs (P>0.05), and they were comparable. The six groups took one solid beverage each time, once a day, for 12 weeks. The efficacy observation was evaluated by fatigue, pale complexion, dizziness, sleep status, and the presence or absence of colds. Improvement of 1-2 indicators was effective, and improvement of 3-4 indicators was markedly effective. After 12 weeks of treatment, the results were comprehensively evaluated through questionnaire collection and self-reporting, and the results are listed in Table 3.

[0087] Table 3 Immunity improvement after 12 weeks of treatment

[0088]

[0089]

[0090] It can be seen from Table 3 that compared with the blank group, all five test groups were effective, but the total effective rate of the QFP group reached 93%, which was higher than that of the other five groups. On the one hand, it shows that the present invention has good effects in replenishing qi and blood, relieving fatigue, and improving sleep, and can significantly enhance the human immune function. On the other hand, it shows that the AF compound product adopted by the present invention effectively regulates the human body as a whole in terms of replenishing qi and blood, nourishing yin and strengthening yang, astringing and consolidating essence, and regulating the spleen and stomach, and has a more prominent effect on enhancing the human immune function.

[0091] Solution:

[0092] Traditional Chinese medicine believes that when the human body is healthy, the qi and blood are full, the yin and yang are balanced, and the person is energetic, physically strong, and quick-witted. When the qi and blood are insufficient and the yin and yang are out of balance, the human body will experience various "sub-health" symptoms, such as fatigue, shortness of breath, palpitations, insomnia, forgetfulness, cold hands and feet, irregular menstruation, loss of appetite, indigestion, hair loss, constipation, etc. The reason is that qi and blood are the source of human immunity. Qi is the commander of blood, and qi can circulate blood and absorb blood; blood is the mother of qi, and blood can generate qi and transform qi. Both are indispensable. To enhance immune function, we must first replenish qi and blood. In this prescription, astragalus, ginseng, and codonopsis are the main qi-replenishing medicines, and angelica, jujube, and longan meat are good blood-replenishing medicines. As the main medicine, the two work together to replenish qi and blood.

[0093] According to traditional Chinese medicine, the kidney is the foundation of the innate nature, the root of life, and has the closest relationship with immunity. The kidney stores essence and qi, transforms into vital energy, maintains the growth and development of the human body, and is the driving force of human life activities. If the kidney yang is insufficient, the five internal organs will lose warmth, which will lead to stagnation of qi and blood, and the tendons and veins will not be nourished. For a long time, it will affect the heart, lungs, liver and other organs, causing organ dysfunction. In this prescription, Cistanche, Eucommia ulmoides leaves, and Alpinia oxyphylla have the function of warming and nourishing kidney yang, and are ministerial drugs. Among them, Cistanche has a sweet and salty taste and warm nature, and has the effect of nourishing kidney yang and benefiting essence and blood; Eucommia ulmoides leaves are pungent and warm in nature, and have the effect of nourishing liver and kidney and strengthening tendons and bones; Alpinia oxyphylla has a pungent and warm nature, and has the effect of warming kidney and consolidating essence. The three medicines can be used alone or in combination to help the smooth flow of qi and blood. However, the medicinal properties of yang-tonifying drugs are mostly dry and strong, which can easily help fire and injure yin, so they are often used in combination with yin-tonifying drugs. In this prescription, lily, polygonatum, wolfberry, and mulberry are both medicinal and edible yin-tonifying drugs. Lily tastes sweet, slightly bitter, and slightly cold. It enters the heart meridian and lung meridian, and has the effects of nourishing yin and moistening the lungs, clearing the heart and calming the mind; polygonatum tastes sweet and is neutral in nature, and has the functions of nourishing qi and yin, strengthening the spleen and moistening the lungs, and benefiting the kidneys; wolfberry tastes sweet and is neutral in nature, and is mainly used to treat liver and kidney yin deficiency, dizziness, tinnitus, blurred vision and other symptoms caused by deficiency of liver and kidney yin and insufficient essence and blood; mulberry is sweet and sour in nature, and enters the heart, liver, and kidney meridians, and has the functions of nourishing yin and moistening dryness, promoting body fluid and quenching thirst. When all the drugs are used together, the monarch and the minister complement each other, so that yang is assisted by yin, yin and yang are balanced, and the body can maintain a healthy state.

[0094] Traditional Chinese medicine believes that when the qi is weak and the biochemical power is weak, it can affect the production of body fluids, resulting in insufficient body fluids. A large loss of body fluids can lead to dryness of body fluids and blood, which is often manifested clinically as spontaneous sweating, night sweats; spermatorrhea, enuresis, frequent urination; prolonged leucorrhea, hemorrhage and metrorrhagia. While nourishing qi and blood, it should be treated with astringent and astringent drugs to achieve both treating the symptoms and strengthening the root cause. Cornus officinalis and raspberry are drugs with astringent and astringent functions as the main functions. Among them, Cornus officinalis is sour and astringent in nature and slightly warm in temperature. It has the effects of nourishing the liver and kidneys, astringing essence and consolidating the body. It is often used for dizziness and tinnitus, sore waist and knees, impotence and spermatorrhea, sweating and collapse, internal heat and thirst; raspberry is sweet, sour and warm in nature and has the functions of nourishing the kidney, consolidating essence and reducing urine, nourishing the liver and improving eyesight. It is often used to treat kidney deficiency, spermatorrhea, enuresis and frequent urination.

[0095] Li Dongyuan, a famous doctor in the Jin and Yuan Dynasties, believed that "internal damage to the spleen and stomach will lead to all kinds of diseases." Therefore, if the spleen and stomach are damaged, the qi and blood will be insufficient, which will not be enough to maintain the normal physiological functions of the internal organs and will not be enough to resist the invasion of pathogens, thus causing various diseases. Modern medicine believes that the spleen and stomach are the foundation of acquired constitution and are responsible for the absorption and metabolism of nutrients. Therefore, if the spleen and stomach function is out of balance, it will affect the nourishing effect of the above-mentioned qi and blood tonics, resulting in various diseases. In the prescription, Poria cocos is sweet and light in taste, and has the effects of invigorating the spleen and stomach, dispelling dampness and promoting diuresis, and is often used to treat urinary dysfunction, edema, spleen deficiency and poor appetite, and loose stools and diarrhea; Chinese yam is sweet and flat in nature, and has the effects of invigorating the spleen and lungs, benefiting the stomach and harmonizing the middle, and is mainly used to treat spleen and stomach weakness, fatigue and weakness, loss of appetite, and chronic diarrhea and dysentery; Hawthorn is flat in nature, sour and sweet in taste, and has the effects of invigorating the spleen and appetite, digesting food and removing stagnation, and is often used to treat abdominal distension, belching and acid regurgitation, abdominal pain and loose stools caused by food stagnation; Tangerine peel is pungent and bitter in taste, warm in nature, and has the effects of regulating qi and invigorating the spleen, drying dampness and removing phlegm, and is used for spleen and stomach qi stagnation syndrome. In addition, because this product has a strong effect of promoting qi and regulating qi, it is used as an adjuvant of tonic medicine in this prescription, which can dredge qi and blood, replenish without stagnation, and prevent congestion and swelling.

[0096] In summary, the combination of the above prescriptions and medicines can replenish qi and blood, balance yin and yang, strengthen the spleen and stomach, and have a strong effect on improving immune function. Compared with general simple tonic Chinese medicines, the new prescription has better efficacy, and as a food, it has a broader market space.

[0097] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a Chinese medicine composition having the effect of enhancing immune function, characterized in that: The Chinese medicine composition is composed of parts A to F; The weight proportions of the medicinal materials in parts A to F are as follows: part A is 1 to 50 parts of astragalus, 1 to 50 parts of ginseng, and 1 to 50 parts of codonopsis; part B is 1 to 40 parts of angelica, 1 to 40 parts of jujube, and 1 to 40 parts of longan pulp; part C is 1 to 30 parts of cistanche, 1 to 30 parts of eucommia leaves, and 1 to 30 parts of alpinia oxyphylla; part D is 1 to 20 parts of lily, 1 to 20 parts of polygonatum, 1 to 20 parts of wolfberry, and 1 to 20 parts of mulberry; part E is 1 to 20 parts of cornus officinalis and 1 to 20 parts of raspberry; part F is 1 to 10 parts of tuckahoe, 1 to 10 parts of yam, 1 to 10 parts of hawthorn, and 1 to 10 parts of dried tangerine peel; The preparation method is as follows: S1. Mix the corresponding medicinal materials in parts A to F; S2, extracting the mixed medicinal materials with water 6 to 60 times the weight of the mixed medicinal materials 2 to 3 times, each time for 20 to 120 minutes to obtain the corresponding extract; S3, combining the two extracts, concentrating under reduced pressure, and obtaining the Chinese medicine composition.

2. A method for preparing a Chinese medicine composition having an immune-enhancing effect as claimed in claim 1, characterized in that: In step S2, an extract is obtained by heating extraction or ultrasonic extraction. The temperature of the heating extraction is maintained at 50-100° C., and the temperature of the ultrasonic extraction is maintained at 20-70° C.

3. A method for preparing a Chinese medicine composition having an immune-enhancing effect as claimed in claim 1, characterized in that: In step S3, the combined extract is concentrated under reduced pressure to an extract having a weight ratio of 1:0.5 to 1:3 to the medicinal material, thereby obtaining the Chinese medicine composition; or the combined extract is concentrated under reduced pressure to a solution having a relative density of 1.05 to 1.20, and the Chinese medicine composition is obtained after spray drying.

4. The method for preparing a Chinese medicine composition having the effect of enhancing immune function as claimed in claim 1, characterized in that: The temperature range of the reduced pressure concentration is 50-70°C.

5. The method for preparing a Chinese medicine composition having the effect of enhancing immune function as claimed in claim 1, characterized in that: In step S2, mulberries, wolfberries, longan pulp and raspberries are extracted by squeezing fresh fruit juice and then spray drying.

6. A Chinese medicine composition having the effect of enhancing immune function, characterized in that: The Chinese medicine composition is prepared by the method for preparing a Chinese medicine composition with the effect of enhancing immune function as claimed in any one of claims 1 to 5; the Chinese medicine composition is composed of parts A to F; wherein the weight portions of the medicinal materials in parts A to F are as follows: part A is 1 to 50 parts of astragalus, 1 to 50 parts of ginseng, and 1 to 50 parts of codonopsis; part B is 1 to 40 parts of angelica, 1 to 40 parts of jujube, and 1 to 40 parts of longan pulp; part C is 1 to 30 parts of cistanche, 1 to 30 parts of eucommia leaves, and 1 to 30 parts of alpinia oxyphylla; part D is 1 to 20 parts of lily, 1 to 20 parts of polygonatum, 1 to 20 parts of wolfberry, and 1 to 20 parts of mulberry; part E is 1 to 20 parts of cornus officinalis and 1 to 20 parts of raspberry; Part F is composed of 1-10 parts of Poria cocos, 1-10 parts of Chinese yam, 1-10 parts of hawthorn, and 1-10 parts of dried tangerine peel.

7. Use of a Chinese medicine composition having an immune-enhancing effect in the preparation of a health food for enhancing immunity, characterized in that: The Chinese medicine composition is prepared by the method for preparing a Chinese medicine composition with immune enhancement effect as described in any one of claims 1 to 5.

8. Use of a Chinese medicine composition having an immune-enhancing effect as claimed in claim 7 in the preparation of a health food for enhancing immunity, characterized in that: The health food prepared from the Chinese medicine composition includes solid beverages, compressed candies, pastes, honey pills, functional jelly and biscuits.

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