Sweet osmanthus lotion with soothing, moisturizing and improving dull yellowness and preparation method thereof

Abstract

The invention discloses an osmanthus emulsion with soothing, moisturizing and improving dull yellowing, comprising natural plant extracts and auxiliary materials; the natural plant extracts comprise the following components by weight percentage: 0.5-2.0% of osmanthus extract, 0.2-1.0% of nymphaeaceae flower extract, and 0.05-0.8% of tuberose polysaccharide. The osmanthus extract, nymphaeaceae flower extract, and tuberose polysaccharide are rationally matched, can effectively remove DPPH free radicals, can reduce the level of glycosylation end products, can effectively improve the dull yellowing of the skin caused by various factors such as skin oxidation, saccharification, melanin deposition, blue light radiation, etc., and the anti-inflammatory component thereof has a soothing and calming effect, can reduce skin irritation and inflammatory reaction, and is helpful to improve skin problems caused by external stimuli.

Description

Technical Field

[0001] The invention relates to the technical field of daily cosmetics, and in particular to an osmanthus emulsion with the functions of soothing, moisturizing and improving dullness and a preparation method thereof. Background Art

[0002] With the increase of physiological age, the increase of environmental pollution, the increase of life pressure and the accelerated pace of life, the skin problems of modern people are becoming more and more serious. The problem of dull skin is a relatively common skin appearance problem worldwide, affecting the appearance and self-confidence of many people. This problem is not limited by age, gender or race, and therefore exists widely in all populations. There are many factors that cause the problem of dull skin, including (1) bad eating habits, irregular lifestyle, and long-term exposure to computer screens, which will cause the skin to produce free radicals and cause aging of the stratum corneum, (2) excessive deposition of melanin in the skin, (3) glycation, the glycation of the dermis will cause the skin to gradually turn yellow, (4) improper skin care and some internal health problems, etc. These factors may affect the health and appearance of the skin to varying degrees, causing the skin to appear dull, dark yellow or lack luster.

[0003] With the continuous advancement of science and technology, the skin care industry is also constantly innovating. New technologies and ingredients are constantly being introduced into skin care products, providing more options for improving skin dullness, and skin care products targeting skin dullness are also emerging. However, some skin care products may have adverse reactions on people with sensitive skin or specific skin types, such as causing allergies or irritation. Products on the market that have the function of brightening skin color mostly use vitamin C, fruit acid, kojic acid, retinoic acid, etc. These raw materials can easily cause redness, swelling, fever, itching, stinging and other problems on sensitive skin, which may make people with sensitive skin unable to obtain the expected improvement effect from these products. Some skin care products targeting skin dullness are expensive, making it difficult for some consumers to afford the cost, thus affecting the popularity of the products. Therefore, there is an urgent need to develop a product that can soothe the skin and safely and effectively improve skin dullness. Summary of the invention

[0004] In view of this, the present invention provides an osmanthus lotion with soothing, moisturizing and improving dull yellowness and a preparation method thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A sweet-scented osmanthus lotion with soothing, moisturizing and yellowing-improving effects comprises natural plant extracts and auxiliary materials; the natural plant extracts comprise the following components by weight percentage: 0.5-2.0% sweet-scented osmanthus extract, 0.2-1.0% niaelendula flower extract, and 0.05-0.8% tuberose polysaccharide.

[0007] Preferably, the natural plant extract comprises the following components by weight percentage: 1.0-1.5% of osmanthus extract, 0.5-0.8% of Niaouli flower extract, and 0.2-0.5% of tuberose polysaccharide.

[0008] Osmanthus fragrans flower extract comes from Osmanthus fragrans Lour., a plant of the genus Oleaceae, and is rich in aromatic substances, such as -acid lactone, a-ionone, 3-ionone, trans-linalool oxide, cis-linalool oxide, linalool, aldehydes, as well as B-phellandrene, nerol, geraniol, and dihydro-B-ionone.

[0009] Melaleuca viridiflora (MELALEUCA LEUCADENDRON VIRIDIFLORA) flower extract is derived from Melaleuca viridiflora (scientific name: Melaleuca viridiflora), which is rich in eucalyptol, terpineol, linalool terpenes - pinene, limonene, pinene, melaleuca, caryophyllene, terpinene, humulene, terpinene, allobellene, camphene, various ester ketones and menthone, and has multiple biological activities.

[0010] Tuberose (POLIANTHES TUBEROSA) polysaccharide is a polysaccharide extracted from the plant Polianthes tuberosa L., which has multiple biological activities and medicinal values. Tuberose polysaccharide is mainly composed of multiple monosaccharide units, such as glucose, xylose, mannose, etc., and has multiple biological activities.

[0011] Preferably, the auxiliary materials include the following components by weight percentage: 1-3% glycerol, 3-7% propylene glycol, 0.02-0.2% sodium hyaluronate, 0.1-0.5% allantoin, 0.5-2% soy lecithin, 0.1-0.3% xanthan gum, 0.01-0.10% chelating agent, 0.1-1% p-hydroxyacetophenone, 0.05-0.2% chlorphenesin and the balance water.

[0012] Preferably, the chelating agent is trisodium ethylenediamine disuccinate.

[0013] Preferably, the present invention also provides a method for preparing an osmanthus lotion having the effects of soothing, moisturizing and improving dull yellowness, comprising the following steps:

[0014] S1. preparing osmanthus extract: crushing osmanthus, mixing with water, distilling under reduced pressure, filtering, concentrating, and drying to obtain osmanthus extract;

[0015] S2. preparing Niaouliu flower extract: crushing Niaouliu flower, mixing with water, heating by microwave, filtering, concentrating, and drying to obtain Niaouliu flower extract;

[0016] S3, mixing the osmanthus extract, the niaouli flower extract, and the tuberose polysaccharide evenly to obtain a natural plant extract;

[0017] S4. Add glycerin, sodium hyaluronate and allantoin to propylene glycol, stir evenly at 70-85° C. until completely dissolved, and continue to add soy lecithin, xanthan gum and trisodium ethylenediamine disuccinate while keeping warm, and stir evenly;

[0018] S5, cooling to 50-55°C, adding the natural plant extract obtained in S3 to the mixture obtained in step S4, and stirring evenly;

[0019] S6, continue to cool to 35-40°C, add p-hydroxyacetophenone, chlorphenesin and water to the mixture obtained in step S5, and homogenize;

[0020] S7, filter and vacuum degas.

[0021] Preferably, the solid-liquid ratio of the S1 osmanthus to water is 1:(10-15) g / mL; the temperature of the reduced pressure distillation is 75-85° C., the time is 2-3 h, and the pressure is -0.05--0.08 MPa.

[0022] Preferably, the liquid ratio of the S2 Melaleuca alternifolia flower to water is 1:(8-12) g / mL; the microwave power is 700-900 Hz, the temperature is 60-75° C., and the time is 2-3 h.

[0023] Preferably, the S6 homogenization speed is 1200-2500r / min, and the homogenization time is 25-40min.

[0024] Preferably, the vacuum degree of the S7 vacuum degassing is 0.5-0.8 MPa.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The present invention provides an osmanthus emulsion with the functions of soothing, moisturizing and improving skin yellowing. The osmanthus extract, the nymphaeaceae flower extract and the tuberose polysaccharide are reasonably matched, and the DPPH free radicals can be effectively scavenged, the level of glycosylation end products can be reduced, and the skin yellowing caused by various factors such as skin oxidation, saccharification, melanin deposition, blue light radiation, etc. can be effectively improved. In addition, the anti-inflammatory component thereof has a soothing and calming effect, can reduce skin irritation and inflammatory reaction, and is helpful to improve skin problems caused by external stimuli. The product of the present invention has a good soothing effect, will not cause skin allergies, and can reduce consumers' adverse reactions during use. DETAILED DESCRIPTION

[0027] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0028] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0029] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0030] The preparation method of the tuberose (POLIANTHES TUBEROSA) polysaccharide used in the examples and comparative examples of the present invention comprises the following steps:

[0031] The tuberose was crushed and added into pure water with a solid-liquid ratio of powder to pure water of 1:10 g / mL; then, the tuberose was extracted at 50°C for 30 min; the extraction was repeated 3 times to obtain an extract, 5 times the volume of anhydrous ethanol was added into the extract, and the extract was precipitated at 5°C for 24 h, the supernatant was collected and filtered, the filtrate was concentrated and dried to obtain tuberose polysaccharide powder.

[0032] Example

[0033] The emulsions of Examples 1-5 and Comparative Examples 1-4 were prepared according to the components and contents (unit: weight %) shown in Table 1-2 below:

[0034] Table 1:

[0035]

[0036]

[0037] Table 2:

[0038]

[0039] The preparation method of the osmanthus emulsion of the above embodiment and comparative example comprises the following steps:

[0040] S1. Preparation of osmanthus extract: fresh osmanthus flowers are washed, dried, crushed, and placed in a distillation flask of a rotary evaporator. Deionized water is added to the distillation flask, and the material-liquid ratio of osmanthus powder to deionized water is 1:10 g / mL. Subsequently, the osmanthus powder is distilled under reduced pressure at 80° C. and −0.06 MPa for 2 h, and the speed of the rotary evaporator is controlled to be 400 r / min. Repeat the above operation to perform secondary distillation, mix the distillates of the two distillations, filter, concentrate, dry, and sterilize to obtain an osmanthus extract;

[0041] S2. Preparation of Niaouliu flower extract: taking Niaouliu flower, washing, drying, crushing, mixing with water, the solid-liquid ratio of Niaouliu flower to water being 1:10 g / mL, placing in a microwave heater, the microwave power being 800 Hz, the temperature being 70° C., the microwave extraction time being 2-3 h, filtering, concentrating, drying, and sterilizing to obtain Niaouliu flower extract;

[0042] S3, mixing the osmanthus extract, the niaouli flower extract, and the tuberose polysaccharide evenly to obtain a natural plant extract;

[0043] S4. Add glycerin, sodium hyaluronate and allantoin to propylene glycol, stir evenly at 80° C. until completely dissolved, and continue to add soy lecithin, xanthan gum and trisodium ethylenediamine disuccinate while keeping warm, and stir evenly;

[0044] S5, cooling to 50-55°C, adding the natural plant extract obtained in S3 to the mixture obtained in step S4, and stirring evenly;

[0045] S6, continue to cool to 35-40 ° C, add p-hydroxyacetophenone, chlorphenesin and water to the mixture obtained in step S5, and homogenize at a homogenization speed of 2000 r / min for 30 min;

[0046] S7, filter, and vacuum degas under the condition of vacuum degree of 0.6 MPa.

[0047] Test Example 1 Antioxidant Performance Test

[0048] DPPH free radical (1,1-diphenyl-2-trinitrophenylhydrazine) is a very stable free radical, which is often used to evaluate the in vitro antioxidant activity of active substances. The greater the free radical scavenging rate, the stronger the antioxidant capacity. Improving the antioxidant capacity of the skin can effectively reduce oxidative stress and improve the problem of dull skin.

[0049] The sample to be tested is a natural plant extract obtained by mixing osmanthus extract, Melaleuca alternifolia flower extract and tuberose polysaccharide obtained in step 3 of Example 1-5 and Comparative Example 1-4. The sample was diluted with DMSO to a sample to be tested with a mass fraction of 2%, and the following experiment was performed: 2.0 mL of DPPH test solution (DPPH 1 mg dissolved in 20 mL ethanol, ultrasonic for 5 minutes, and fully shaken) and a certain amount of the sample to be tested were added to a test tube, and the total volume was supplemented to 3 mL with anhydrous ethanol, shaken well, and reacted in the dark for 20 minutes, and the absorbance was measured at a wavelength of 517 nm, which was recorded as A0; 2 mL of anhydrous ethanol and a corresponding volume of the sample to be tested were added to the test tube, and the total volume was supplemented to 3 mL with anhydrous ethanol, and the measured absorbance was recorded as A1; DPPH test solution (DPPH 1 mg was dissolved in 20 ml of ethanol, ultrasonicated for 5 minutes, and shaken thoroughly) 2 mL and 1 mL of anhydrous ethanol, the measured absorbance was recorded as A2, and the DPPH free radical scavenging rate (%) of the test solution was calculated; the calculation formula for the DPPH free radical scavenging rate is: scavenging rate (%) = [1-(A0-A1) / A2]*100%, and the test results are shown in Table 3.

[0050] Table 3

[0051]

[0052]

[0053] It can be seen from Table 3 that, compared with the comparative example, the natural plant extract obtained by uniformly mixing the osmanthus extract, the niaouli flower extract and the tuberose polysaccharide obtained in the embodiment of the present invention has obvious DPPH free radical scavenging ability.

[0054] Test Example 2 Anti-glycation performance test

[0055] Advanced glycation end products (AGEs) refer to a class of stable compounds produced by a series of non-enzymatic reactions such as condensation, rearrangement, cleavage, and oxidation modification of reducing sugar carbonyl groups with amino acids or free amino acids of macromolecules such as proteins, lipids, or nucleic acids. The accumulation of AGEs on the skin will cause cross-linking damage to proteins and binding to specific receptors, causing many skin problems, such as dull skin, decreased collagen fiber elasticity, dark brown spots, and even promoting the production of matrix metalloproteinases that can decompose collagen and elastin, as well as increasing the secretion of cytokines that can cause inflammatory reactions. Experimental principle: The modeling process is based on the principle that glucose and bovine serum albumin undergo glycation reaction in vitro to form AGEs. The anti-glycation effect is evaluated by adding the sample to be tested and calculating the AGE inhibition rate by fluorescence quantitative method.

[0056] The sample to be tested is a natural plant extract obtained by mixing osmanthus extract, Melaleuca alternifolia flower extract and tuberose polysaccharide in step 3 of Example 1-5 and Comparative Example 1-4. Test method: (1) A liquid containing 5% bovine serum albumin and 1 mol / L glucose at a final concentration of 5% by volume is prepared with a phosphate buffer having a pH value of 7.4, and the mixture is incubated continuously for 5 days at a constant temperature, aerobic and light-proof conditions at 55°C, and the OD value of the formed AGE is determined by an enzyme marker; (2) A mixed incubation liquid containing the natural plant extracts prepared in Example 1-5 and Comparative Example 1-4 is prepared with a liquid containing 5% bovine serum albumin and 1 mol / L glucose. The control group is a blank control, and no natural plant extract is added. The mixture is incubated continuously for 5 days at a constant temperature, aerobic and light-proof conditions at 55°C, and the OD value of the formed AGE is determined by an enzyme marker. AGE inhibition rate = (CT) / C×100, wherein C is the fluorescence intensity of the control group (blank case), that is, the intensity of glycated bovine serum albumin in the absence of natural plant extracts; T is the intensity of glycated bovine serum albumin when a natural plant extract obtained by mixing osmanthus extract, Melaleuca alternifolia flower extract, and tuberose polysaccharide is added; the inhibitory effects of the test samples on AGE are shown in Table 4.

[0057] Table 4

[0058] Group AGE inhibition rate (%) Example 1 85.4 Example 2 86.0 Example 3 85.8 Example 4 85.7 Example 5 85.5 Comparative Example 1 79.6 Comparative Example 2 72.2 Comparative Example 3 71.8 Comparative Example 4 62.5

[0059] As can be seen from Table 4, compared with the comparative example, the natural plant extract obtained by uniformly mixing osmanthus extract, niaouli flower extract and tuberose polysaccharide obtained in the embodiment of the present invention has a significant inhibitory effect on AGE and has an obvious anti-glycation effect.

[0060] Test Example 3: Anti-blue light performance test

[0061] The Organotypical skin model was oxidized by blue light radiation. The experimental group was pretreated with the natural plant extract obtained by mixing osmanthus extract, niaouli flower extract, and tuberose polysaccharide obtained in step 3 of the test sample examples 1-5 and comparative examples 1-4, and the control group was not treated and irradiated with blue light. The blue light intensity was 45 J / cm 2 Medium to high doses of ROS (reactive oxygen species) can induce cell apoptosis and even necrosis through cellular oxidative stress response. Therefore, measuring the accumulation of ROS in epidermal keratinocytes can reflect the anti-photoaging ability of the test sample. The test results are shown in Table 5.

[0062] Table 5

[0063]

[0064]

[0065] It can be seen from Table 5 that, compared with the comparative example, the natural plant extract obtained by uniformly mixing osmanthus extract, nymphaeaceae flower extract and tuberose polysaccharide obtained in the embodiment of the present invention can inhibit the generation of ROS in human epidermal keratinocytes under blue light conditions, thereby protecting skin cells from damage by blue light radiation.

[0066] Test Example 4 Melanin Inhibition Performance Test

[0067] The sample to be tested is a natural plant extract obtained by mixing osmanthus extract, green melaleuca extract, and tuberose polysaccharide in step 3 of Example 1-5 and Comparative Example 1-4. Experimental method: B-16 melanoma cells were cultured with 0.1wt% glucosamine, theophylline was added to promote the cells to recover to the state of melanin synthesis, and 2% of the natural plant extract obtained in the embodiment and comparative example was added to the culture medium, and the untreated group was used as the blank group; the color tone of the cell particles was examined under a microscope to determine the inhibitory effect of the sample on the new melanin. The cell particles were centrifuged to release the intracellular particles, and the absorbance was measured at 420nm. The calculation formula of the inhibition rate of melanin is as follows: inhibition rate = 100% - absorbance of the sample to be tested after treatment / absorbance of the blank group * 100%, and the results are shown in Table 6.

[0068] Table 6

[0069] Group Inhibition rate (%) Blank group 0 Example 1 57.6 Example 2 58.2 Example 3 58.3 Example 4 58.2 Example 5 57.9 Comparative Example 1 42.8 Comparative Example 2 32.5 Comparative Example 3 36.1 Comparative Example 4 20.2

[0070] It can be seen from Table 6 that, compared with the comparative example, the natural plant extract obtained by uniformly mixing the osmanthus extract, the niaouli flower extract and the tuberose polysaccharide obtained in the embodiment of the present invention has a higher inhibition rate on the black rate.

[0071] Test Example 5 Anti-inflammatory Performance Test

[0072] The soothing effect of the soothing repair composition was evaluated by testing the effect of the natural plant extract obtained by mixing osmanthus extract, niaouli flower extract and tuberose polysaccharide uniformly obtained in step 3 of Examples 1-5 and Comparative Examples 1-4 on the synthesis of inflammatory factor IL-1B in RAW264.7 cells. A blank group and a sample group were set up, and the test substance in the sample group was the natural plant extract prepared in the examples and comparative examples. Experimental methods: (1) Cell inoculation: RAW264.7 cells were inoculated at 10,000 cells / well in a 96-well plate and cultured in a 37°C, 5% CO2 incubator for 24 h; (2) LPS-induced inflammatory response: 1 mg / mL LPS solution (solvent: PBS buffer) was diluted to 2 μg / mL, added to the plate at 100 μL / well, and cultured in an incubator; (3) Sample addition: Complete culture medium was used as the diluent to prepare the sample working solution, 3 w% of the natural plant extract prepared in the embodiment and the comparative example was added to the sample group, 100 μL / well, and the blank group was added with an equal amount of complete culture medium, and cultured in an incubator for 24 h; (4) The supernatant in the plate was taken and the IL-18 content was detected by ELISA. The test results are shown in Table 7.

[0073] Table 7

[0074] Group IL-18 secretion (ng / mL) Control group 2.59 Example 1 0.60 Example 2 0.57 Example 3 0.55 Example 4 0.58 Example 5 0.62 Comparative Example 1 1.23 Comparative Example 2 1.65 Comparative Example 3 1.54 Comparative Example 4 1.71

[0075] It can be seen from Table 7 that, compared with the comparative example, the natural plant extract obtained by uniformly mixing osmanthus extract, niaouli flower extract and tuberose polysaccharide obtained in the embodiment of the present invention has a significant inhibitory effect on the inflammatory factor IL-18.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sweet-scented osmanthus lotion with soothing, moisturizing and improving dull yellowness, characterized in that: The invention comprises natural plant extracts and auxiliary materials; the natural plant extracts comprise the following components by weight percentage: 0.5-2.0% of osmanthus extract, 0.2-1.0% of niaouli flower extract and 0.05-0.8% of tuberose polysaccharide; The auxiliary materials include the following components by weight percentage: 1-3% glycerol, 3-7% propylene glycol, 0.02-0.2% sodium hyaluronate, 0.1-0.5% allantoin, 0.5-2% soybean lecithin, 0.1-0.3% xanthan gum, 0.01-0.10% trisodium ethylenediamine disuccinate, 0.1-1% p-hydroxyacetophenone, 0.05-0.2% chlorphenesin and the balance water; The method for preparing the sweet osmanthus emulsion having the functions of soothing, moisturizing and improving dull yellowness comprises the following steps: S1. preparing osmanthus extract: crushing osmanthus, mixing with water, distilling under reduced pressure, filtering, concentrating, and drying to obtain osmanthus extract; S2. preparing Niaouliu flower extract: crushing Niaouliu flower, mixing with water, heating by microwave, filtering, concentrating, and drying to obtain Niaouliu flower extract; S3, mixing the osmanthus extract, the niaouli flower extract, and the tuberose polysaccharide evenly to obtain a natural plant extract; S4. Add glycerin, sodium hyaluronate and allantoin to propylene glycol, stir evenly at 70-85° C. until completely dissolved, and continue to add soy lecithin, xanthan gum and trisodium ethylenediamine disuccinate while keeping warm, and stir evenly; S5, cooling to 50-55°C, adding the natural plant extract obtained in S3 to the mixture obtained in step S4, and stirring evenly; S6, continue to cool to 35-40°C, add p-hydroxyacetophenone, chlorphenesin and water to the mixture obtained in step S5, and homogenize; S7, filter and vacuum degas.

2. The sweet osmanthus lotion having the effects of soothing, moisturizing and improving dull yellowness according to claim 1, characterized in that: The natural plant extract comprises the following components by weight percentage: 1.0-1.5% of osmanthus extract, 0.5-0.8% of niaouli flower extract and 0.2-0.5% of tuberose polysaccharide.

3. The sweet osmanthus lotion having the effects of soothing, moisturizing and improving dull yellowness according to claim 1, characterized in that: In step S1, the material-liquid ratio of osmanthus to water is 1:(10-15) g / mL, the temperature of the reduced pressure distillation is 75-85° C., the time is 2-3 h, and the pressure is -0.05--0.08 MPa.

4. The sweet osmanthus lotion having the effects of soothing, moisturizing and improving dull yellowness according to claim 1, characterized in that: In step S2, the liquid ratio of Melaleuca alternifolia to water is 1:(8-12) g / mL, the microwave power is 700-900 Hz, the temperature is 60-75° C., and the time is 2-3 h.

5. The sweet osmanthus lotion with soothing, moisturizing and improving dull yellowness according to claim 1, characterized in that: In step S6, the homogenization speed is 1200-2500 r / min, and the homogenization time is 25-40 min.

6. The sweet osmanthus lotion with soothing, moisturizing and improving dull yellowness according to claim 1, characterized in that: The vacuum degree of vacuum degassing in step S7 is 0.5-0.8 MPa.

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