High-tolerance fermented food for pet dogs and cats

Abstract

The present application discloses a high-tolerance fermented food for pet dogs and cats, belonging to the technical field of pet food. Chicken meal, corn, corn gluten meal, soybean meal, beet meal, cellulose, calcium carbonate, multivitamin, choline chloride, potassium chloride, chicken fat, and fish oil are added to the food as basic ingredients to reduce materials with high risk of anti-nutritional factors, meet comprehensive nutrition and reduce intestinal burden; and additives are added as regulating ingredients, four bacteria, Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis, can increase the number of beneficial bacteria on the basis of inhibiting the growth of harmful bacteria, and reduce intestinal permeability, which is suitable for improving the tolerance of cats and dogs in old age.

Description

Technical Field

[0001] The present application relates to a high-tolerance fermented food for pet dogs and cats, belonging to the technical field of pet food. Background Art

[0002] There are a variety of microbial communities in the intestines of pet cats and dogs. When the ratio of beneficial bacteria to harmful bacteria is unbalanced, it will affect the digestion and absorption of food by cats and dogs, making intolerance prone to occur, which is manifested in vomiting, diarrhea, indigestion, inflammatory bowel disease, skin diseases, respiratory diseases and other aspects.

[0003] Currently, there are three main treatments for pet intolerance: first, identify and eliminate food ingredients that cause intolerance, and choose alternative foods that do not contain allergens; second, for non-severe cases of intolerance, try to gradually reintroduce the problem food to observe whether the pet can gradually adapt; third, use anti-allergic drugs, anti-inflammatory drugs, etc. under the guidance of a veterinarian to alleviate the pet's clinical symptoms.

[0004] For cats and dogs entering old age, their intestinal tolerance is worse than that of cats and dogs in their prime. When using the first two treatment methods, it is difficult to identify the food ingredients that cause intolerance, and even after gradual adaptation, intolerance may reoccur with the extension of time and the degradation of intestinal function. When using the third treatment method, although it can relieve the pet's symptoms to a certain extent in the present, it cannot fundamentally solve the problem. In addition, long-term use of anti-allergic or anti-inflammatory drugs may disrupt the balance of intestinal microorganisms, which will increase the risk of food intolerance and is not suitable for use in elderly cats and dogs.

[0005] Currently, researchers are committed to studying pet foods that can fundamentally improve pets' tolerance, generally by adding Chinese medicine ingredients, probiotics, etc. to promote intestinal digestion and absorption. Such improvements can play a role in cats and dogs in normal growth or middle age, but in the case of elderly cats and dogs whose intestinal functions are increasingly weakened, the above technical improvements are difficult to play an effective role. Therefore, there is currently a lack of high-tolerance food specifically for elderly cats and dogs. Summary of the invention

[0006] In order to solve the above problems, a high-tolerance fermented food for pet dogs and cats is provided. The application first designs different basic formulas based on the intestines of cats and dogs to reduce materials with high risk of anti-nutritional factors, thereby meeting comprehensive nutrition while reducing the burden on the intestine; and then by adding Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis subsp. lactis, the number of beneficial bacteria can be increased on the basis of inhibiting the growth of harmful bacteria, and the intestinal permeability can be reduced, which is suitable for improving the tolerance of elderly cats and dogs.

[0007] The present application provides a high-tolerance fermented food for pet dogs and cats, which comprises, by weight, 71.9-92.6 parts of basic ingredients, 1.21-3.35 parts of additives, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, and 0.5-1 parts of Lactobacillus delbrueckii lactis, wherein the weight ratio of Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG, and Lactobacillus delbrueckii lactis is 2:1:2:1;

[0008] The base ingredients include chicken meal, corn, corn gluten meal, soybean meal, sugar beet meal, cellulose, calcium carbonate, multivitamin, choline chloride, potassium chloride, chicken fat and fish oil.

[0009] The basic formula of this pet dog and cat food does not contain wheat, which will greatly reduce the probability of pet dogs and cats being allergic. The amount of soybean meal added in this food is relatively small. If a large amount is added, it will cause intestinal intolerance in elderly dogs and cats; the ratio of beet pulp and cellulose can make the ratio of fermented fiber and non-fermented fiber in the food moderate, and the intestinal adaptability is strong; fish oil contains omega-3, EPA and DHA ingredients, which can meet comprehensive nutritional needs; the components and ratios of the remaining substances can not only meet the growth needs of elderly pet dogs and cats, but also facilitate digestion and absorption by pet dogs and cats. Long-term use can enable pet dogs and cats to establish a healthy intestinal system and delay the deterioration of their intestinal function.

[0010] The high-tolerance fermented food for pet dogs and cats of the present application adds four kinds of probiotics on the basis of the basic formula, which can cooperate with each other, improve the nutrient absorption of pet cats and dogs in the elderly to the basic formula, and promote the intestinal health of pet cats and dogs, avoid vomiting and inflammatory bowel disease in pet cats and dogs, reduce diarrhea rate, reduce the probability of occurrence of skin diseases and respiratory diseases, improve immunity, and reduce the natural weight loss rate. And the applicant found that when the weight ratio of Lactobacillus casei Lc12 to Bacillus coagulans exceeds 2:1, the effect of Lactobacillus casei Lc12 in inhibiting harmful bacteria will be weakened, but the tolerance of cats and dogs in the elderly to the food will be reduced. When the weight ratio of Lactobacillus casei Lc12 to Bacillus coagulans is 2:1, not only will the effect of Lactobacillus casei Lc12 not be weakened, but it can also cooperate with Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis subspecies, so that the tolerance of pet cats and dogs in the elderly to the food is significantly improved. Under the above-mentioned weight ratio setting, each bacterial species can play its own role, and can also synergistically improve the tolerance of elderly pet cats and dogs to food, help elderly cats and dogs establish a healthy intestinal digestive system, and delay the degeneration of their intestinal function.

[0011] Among them, Lactobacillus casei LC-12 can firstly inhibit the colonization and growth of harmful bacteria in the intestine by competitive exclusion and production of antibacterial substances, thereby maintaining the integrity of the intestinal mucosal barrier; secondly, it can promote the proliferation and differentiation of intestinal epithelial cells, enhance the repair ability of the intestinal mucosa, and help maintain intestinal health; the applicant found that increased intestinal permeability is one of the important causes of food intolerance and intestinal inflammation. The added Lactobacillus casei LC-12 can thirdly reduce intestinal permeability through multiple mechanisms, including enhancing the integrity of the intestinal mucosal barrier, regulating the expression of tight junction proteins, inhibiting inflammatory responses, etc., thereby improving food intolerance symptoms; fourthly, it can increase the number of beneficial bacteria, reduce the proportion of harmful bacteria, improve the development and function of the intestinal immune system, and achieve a balance of Th1 / Th2 immune responses by regulating the intestinal microbiota; fifthly, it can regulate the balance of T lymphocyte subsets and increase the number of regulatory T cells (Treg) in the intestine, thereby inhibiting excessive Th1 and Th2 immune responses.

[0012] Bacillus coagulans has a strong ability to adhere to intestinal epithelial cells, which is an important condition for Bacillus coagulans to have probiotic functions. Therefore, Bacillus coagulans can firstly adhere to the intestinal mucosa and form a biological barrier with it to protect the host and resist the invasion of foreign microorganisms; secondly, the antibacterial substances such as L-lactic acid, antimicrobial peptides and coagulins secreted during its growth and metabolism can jointly inhibit a variety of pathogenic pathogens in the intestine and reduce the incidence of animals. L-lactic acid can also promote the digestion and utilization of iron, phosphorus, calcium and vitamin D by animals, and the digestive enzymes and various metabolites produced can promote the body's digestion and absorption of nutrients. The secreted protease, lipase, amylase, xylanase, β-galactosidase, lactate dehydrogenase and other digestive enzymes and enzymatic factors can degrade complex carbohydrates and eliminate anti-nutritional factors in pet dog and cat food, improve the digestion and utilization of nutrients by animals, and produce amino acids, vitamins, short-chain fatty acids and growth-promoting factors that can be directly digested and utilized by animals; thirdly, Bacillus coagulans has a broad antibacterial spectrum, has a competitive advantage in coexisting with harmful bacteria, and promotes the health of the host. It can be used against Listeria, Staphylococcus aureus, Escherichia coli, and Sarcoma. It has a significant dissolving effect on S. mucosa, Vibrio parahaemolyticus, Enterococcus faecalis, Vibrio vulnificus and Pseudomonas aeruginosa; fourthly, it can promote intestinal morphological development, improve intestinal morphological structure, and maintain the integrity of the intestinal mucosal barrier function; fifthly, it can promote the proliferation of T and B lymphocytes, stimulate helper T cells to secrete cytokines, enhance the activity of natural killer cells (NK) and macrophages, and improve the level of humoral immunity and cellular immunity; sixthly, it can increase the activity of antioxidant enzymes in the body's serum and liver, reduce the damage of free radicals to the body's cells, and inhibit stress and diseases induced by reactive oxygen.

[0013] The cooperation and synergy of Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis can, firstly, inhibit the colonization and growth of harmful bacteria in the intestine by competitive exclusion and the production of antibacterial substances, thereby maintaining the integrity of the intestinal mucosal barrier; secondly, it can improve the symptoms of food intolerance by regulating the expression of tight junction proteins, enhancing the function of the intestinal mucosal barrier, reducing the damage of harmful substances to the body, inhibiting inflammatory responses, etc.; thirdly, it can regulate the balance of T lymphocyte subsets and increase the number of regulatory T cells in the intestine, thereby inhibiting excessive Th1 and Th2 immune responses; fourthly, it can increase the number of beneficial bacteria, reduce the proportion of harmful bacteria, improve the structure of intestinal flora, alleviate food intolerance, and further affect the composition of intestinal microbiota and metabolites. The development and function of the intestinal immune system, achieving a balance between Th1 / Th2 immune responses, improving the development and function of the intestinal immune system, and further promoting the formation of immune tolerance. For example, increasing the production of beneficial metabolites such as short-chain fatty acids (SCFA) helps maintain intestinal immune homeostasis; fifth, it can inhibit the production of inflammatory mediators by intestinal epithelial cells and immune cells, such as tumor necrosis factor (TNF-α), interleukin (IL), etc., thereby reducing intestinal inflammatory responses; sixth, by enhancing the intestinal barrier function, reducing endotoxin and bacterial translocation, and further reducing the risk of systemic inflammatory responses; seventh, it can promote the differentiation and maturation of immune cells in the intestine, increase tolerance to food antigens, and reduce the occurrence of food intolerance from the root.

[0014] Optionally, the additives include: 0.1-0.3 parts of glutamine, 0.1-0.2 parts of tyrosine, 0.1-0.2 parts of fructo-oligosaccharides, 0.5-1.0 parts of isomaltooligosaccharides, 0.1-0.5 parts of yeast cell walls, 0.1-0.5 parts of betaine, 0.05-0.2 parts of sodium butyrate, 0.05-0.1 parts of bile acid, 0.01-0.05 parts of lactic acid, and 0.1-0.3 parts of citric acid.

[0015] The presence of additives can assist the four strains of bacteria to function, and can also promote the intestinal health of elderly pet cats and dogs, delay the degeneration of their intestinal function, reduce the diarrhea rate and stress response, increase the brightness of the pet cats and dogs' hair, and reduce the occurrence of tear marks.

[0016] Glutamine in the additive can improve the metabolic utilization of Gln by intestinal epithelial cells by increasing the activity of Gln enzyme, and inhibit the apoptosis of mucosal cells. Gln can also increase the synthesis of GSH and reduce the damage of intestinal mucosal cells by limiting the production of cytokines and inflammatory responses; it can promote the proliferation of intestinal epithelial cells by stimulating the expression of insulin-like growth factor-1 (IGF-1) in the liver and intestinal mucosa, accelerate the repair of damaged intestinal mucosa, and maintain the integrity of the intestinal mucosal structure; Glutamine can promote the expression of tight junction proteins and mucins, maintain normal paracellular permeability and epithelial cell barriers, and protect the intestinal mucosal structure and barrier function; Glutamine can effectively increase the number of IgA-producing plasma cells and CD3, CD4+, and CD8+ in the intestinal mucosa, promote the synthesis and secretion of S-IgA, and prevent pathogens from colonizing the mucosa and invading (translocation).

[0017] Tyrosine can increase the growth and repair of intestinal mucosal cells, increase the intestinal barrier function, prevent the invasion of harmful substances, and reduce the occurrence of intestinal inflammation and diseases; at the same time, the tyrosine of the present application can also promote the growth of beneficial bacteria, maintain the balance of intestinal flora, and improve the digestion and absorption function of the intestine.

[0018] First, oligofructose can improve intestinal microbial flora, improve lipid metabolism, enhance the body's immunity, and promote mineral absorption; second, it can synergize with short-chain fatty acids to improve the balance of intestinal flora and promote the proliferation of intestinal bifidobacteria and lactic acid bacteria; third, it can specifically bind to pathogenic microorganisms through oligosaccharide receptors, blocking the adhesion and colonization of harmful bacteria in intestinal mucosal epithelial cells, thereby inhibiting the growth of harmful bacteria. Reduce toxin production and accumulation, and promote normal intestinal peristalsis.

[0019] Isomaltooligosaccharide can promote the proliferation of bifidobacteria in the intestines of dogs and cats, and can inhibit the formation of harmful bacteria and corrupt substances in the intestines.

[0020] Yeast cell walls contain a large amount of β-1,3-D glucan and manno-oligosaccharide, which produce non-specific immunity to diseases caused by bacteria, fungi and viruses, as well as stress responses caused by transportation, vaccination, transfer, climate change, etc. As for the large amount of manno-oligosaccharide it contains, since the oligosaccharide itself has a certain immunogenicity, it can stimulate the body of elderly cats and dogs to produce an immune response; as for the large amount of β-1,3-D glucan it contains, since it is the most important structural material of the cell wall, it has a defensive function against microorganisms invading the animal body. Studies have found that some phosphorylated glucans can bind to the infected pathogenic bacteria, fungi and viruses, inhibiting the reproduction of harmful bacteria. In addition, yeast cell walls are also an important source of oligo-manno-oligosaccharides. Manno-oligosaccharides have the function of capturing harmful bacteria attached to the intestinal wall (such as Escherichia coli and Salmonella) in animals, preventing pathogens from settling and proliferating, and maintaining the established healthy flora of the digestive tract in animals, thereby controlling the occurrence of diseases.

[0021] Since food causes changes in the osmotic pressure of intestinal epithelial cells during the nutrient digestion process, these intestinal cells need to control the exchange of water and different nutrients between the intestinal cavity and plasma. The added betaine has bipolar zwitterionic characteristics, so it has osmotic pressure protection properties and has the effect of alleviating the osmotic pressure changes in the intestine. At the same time, betaine can maintain the intestinal barrier effect and enhance the connection between the tight junction proteins of epithelial cells. Improving the integrity of the intestinal barrier is crucial to preventing the entry of harmful substances and pathogens and causing inflammation.

[0022] Butyrate plays a wide range of roles in the body's immunity. In the colon, butyrate is one of the most abundant bacterial metabolites, which can enter cells through passive diffusion, carrier-mediated transport, and interaction with GPCRs (G protein-coupled receptors). Adding sodium butyrate can regulate the epidermal barrier, mediate inflammation through the host's natural immunity, and inhibit intestinal pathogen infection.

[0023] Adding bile acid can, firstly, promote fat digestion, promote liver function recovery, regulate intestinal flora, and restore liver-intestinal homeostasis; secondly, it can activate the activity of pancreatic lipase and intestinal lipase, promote the full digestion and absorption of oil, solve the problems of incomplete oil digestion, excessive reproduction of oil-eating microorganisms in the intestine, imbalance of intestinal microflora, and reduce fatty diarrhea; thirdly, it can stimulate the secretion of bile, unclog the bile duct, eliminate mycotoxins, bacterial endotoxins, heavy metals and other harmful substances, thereby reducing the absorption of harmful substances in dogs and cats and reducing damage to the intestines and livers of dogs and cats; effectively improve the animal's absorption and utilization of fat, fat-soluble vitamins and cholesterol.

[0024] The added lactic acid and citric acid can regulate the gastric acid secretion of pets, increase the acidity of gastric acid, and promote the digestion and absorption of food; they can also inhibit the growth of harmful bacteria, reduce the occurrence of gastrointestinal infections and diseases, and play an important regulatory role in the digestive system, immune system and intestinal health of elderly cats and dogs; at the same time, they can also improve the shelf life and flavor of pet food.

[0025] Preferably, the amount of glutamine used accounts for 5% of the total weight of the four bacterial species of Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii subsp. lactis.

[0026] The applicant found that under the respective effects of glutamine and the four bacterial species, the combined use of glutamine and the four bacterial species in the above dosage settings can reduce the synthesis and release of NO by enhancing the methylation level of the iNOS gene, thereby inhibiting intestinal oxidative stress and inflammatory response, and is more suitable for use in elderly pet cats and dogs.

[0027] The weight ratio of fructooligosaccharide to isomaltooligosaccharide in the above additives is 1:5, and the weight ratio of betaine, lactic acid and citric acid is 10:1:5.

[0028] Isomaltooligosaccharide and fructooligosaccharide will not decompose under acidic conditions at room temperature. When used together in the above-mentioned weight ratio, they can play to their strengths and avoid their weaknesses. They can significantly proliferate a variety of beneficial bacteria, such as lactobacillus and bifidobacterium, to help elderly pet cats and dogs establish a healthy intestinal environment.

[0029] Since betaine is also a safe source of hydrochloric acid, at the above weight ratio, betaine works synergistically with lactic acid and citric acid to act as an acidifier, helping to activate pepsin, promoting digestion and absorption of nutrients, and is more beneficial for consumption by elderly pet cats and dogs.

[0030] Optionally, the viable count of Lactobacillus casei Lc12 is 5×10 8 -10×10 8 cfu / g, and the viable bacterial count of the Bacillus coagulans was 3×10 8 -8×10 8 cfu / g, and the viable bacterial count of the rhamnosus lactobacillus GG was 5×10 8 -10×10 8 cfu / g, and the viable count of the Lactobacillus delbrueckii subspecies lactis was 5×10 8 -10×10 8 cfu / g.

[0031] The high-tolerance fermented food for pet cats of the present application comprises, by weight: 30-32 parts of chicken meal, 5-8 parts of anchovy meal, 16-20 parts of corn, 3-6 parts of corn gluten meal; 20-24 parts of rice, 8-10 parts of cassava starch, 5-8 parts of soybean meal, 3-5 parts of beet meal, 2-4 parts of cellulose, 0.3-0.5 parts of taurine, 0.2-0.4 parts of calcium carbonate, 1-2 parts of multivitamin, 0.5-0.8 parts of choline chloride, 0.2-0. 4 parts of potassium chloride, 9.5-12 parts of chicken fat, 1.5-2 parts of fish oil, 5-7 parts of cat pulp, 1-2 parts of cat powder, 1.21-3.35 parts of additives, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, and 0.5-1 part of Lactobacillus delbrueckii lactis, wherein the weight ratio of Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis is 2:1:2:1.

[0032] Anchovy meal is added to the pet cat food, which can provide rich nutrients and increase the content of lysine, methionine and tryptophan in the food, and is more suitable for consumption by elderly pet cats.

[0033] The preparation method of the high-tolerance fermented food for pet cats comprises the following steps:

[0034] (1) fermenting corn and soybean meal with 1 part of Lactobacillus casei Lc12 and 0.5 part of Liposaccharomyces staryi and enzymolysis to obtain a fermentation product;

[0035] (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives to obtain a first premix;

[0036] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, cat pulp, cat powder, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed, and then crushed and passed through a 0.8 mm sieve, and then expanded and dried to obtain a second premix;

[0037] (4) Mix chicken oil and fish oil and spray them onto the surface of the second premix, and then spray cat paste and cat powder in sequence. After cooling, the mixture is obtained.

[0038] The applicant found that when other substances are added, directly adding corn and soybean meal in the food will increase the granularity of the food, resulting in low digestibility of dry matter, crude protein and organic matter for pet cats and dogs in the old age, and may also cause pet constipation and aggravation of fecal odor. In order to improve this problem, Lactobacillus casei Lc12 in the present application is used to ferment corn and soybean meal to obtain fermented product, which can improve the digestibility of dry matter, crude protein and organic matter for pet cats and dogs in the old age, and after research, it is found that fermentation with composite Starch grease yeast can further improve the digestibility, and the enzymolysis process used after fermentation can not only make the material after corn and soybean meal fermentation decompose into small molecules that are easily absorbed, but also can play the role of increasing the number of beneficial bacteria and reducing the number of harmful bacteria.

[0039] Optionally, the specific fermentation method for obtaining the fermented product after the corn and soybean meal in step (1) are fermented and enzymatically hydrolyzed by 1 part of Lactobacillus casei Lc12 and 0.5 part of Starburst yeast is:

[0040] S1: cooking corn and soybean meal in 30-50 times water, and filtering to obtain residue and filtrate;

[0041] S2: Mix the culture medium and the filter residue in a weight ratio of 1:(4-5), then add the filtrate 2-3 times the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 25-35°C for 4-5 days, sterilize and filter to obtain the fermentation liquid;

[0042] S3: Add 0.5 parts of aminopeptidase to the fermentation broth, perform enzymolysis at 60°C and pH 8.0 for 1-3 hours, then add 0.5 parts of saccharifying enzyme, perform enzymolysis at 55°C and pH 6.0 for 2-3 hours, and inactivate the enzyme at high temperature to obtain the fermentation product.

[0043] The purpose of cooking in step S1 is to soften the epidermis of corn and soybean meal, reduce the oil content thereof, avoid the effect of oil hindering the strain, and facilitate the smooth progress of fermentation and enzymolysis; it has been verified that the filtrate added in step S2 can increase the amount of Lactobacillus casei Lc12 and Starch yeast infiltrating into the cell walls of corn and soybean meal, thereby improving the fermentation efficiency and increasing the content of nutrients in the fermented product; if no filtrate is added, the content of nutrients is greatly reduced; in step S3, aminopeptidase is used for the first enzymolysis to quickly hydrolyze the nutrients into small molecules, and saccharifying enzyme is used for the second enzymolysis to hydrolyze the small molecules into peptides that are easily absorbed by elderly pet cats and dogs and substances that promote the reproduction of beneficial bacteria, the easily absorbed peptides can improve the digestibility of dry matter, crude protein and organic matter, the substances that promote the reproduction of beneficial bacteria can increase the number of beneficial bacteria in cooperation with other food substances, and the number of beneficial bacteria increases while competing with harmful bacteria for nutrients, thereby reducing the number of harmful bacteria.

[0044] The above fermentation method can improve the digestion and absorption rate of food for elderly pet cats and dogs, increase the number of beneficial bacteria and reduce the number of harmful bacteria, reduce the constipation and diarrhea rate of pet cats and dogs, and reduce the fecal odor of pet cats and dogs.

[0045] Optionally, the bacterial culture medium includes 2-3 g of peptone, 1-2 g of white sugar, 1.5-2 g of yeast powder, 0.5-1 g of potassium dihydrogen phosphate, and 100-120 g of purified water. The mixture is stirred evenly and sterilized at 121° C. for 20 min before use.

[0046] Optionally, the mixing time of step (2) is 80-120 s, and the mixing time of step (3) is 100-140 s.

[0047] Optionally, the puffing temperature in step (3) is 90-130° C., and the bulk density after puffing is 370-390 g / L.

[0048] Optionally, the drying temperature in step (3) is 105-110° C., and the drying is performed until the moisture content is 5.5-7.0%.

[0049] Optionally, in step (4), the temperature of the chicken oil and fish oil sprayed is 45-50°C, the temperature of the cat paste sprayed is 45-50°C, the temperature of the cat powder sprayed is room temperature, and the spraying time of the entire step S3 does not exceed 2 minutes.

[0050] Optionally, the cooling in step (4) is performed by cooling with air at normal temperature.

[0051] The high-tolerance fermented food for pet dogs of the present application comprises, by weight: 19-22 parts of chicken meal, 24-27 parts of corn, 6-8 parts of corn gluten powder, 27-30 parts of rice, 1-2 parts of duck blood globulin powder, 8-10 parts of sweet potato granules, 6-8 parts of soybean meal, 3-5 parts of beet meal, 2-4 parts of cellulose, 0.7-1.0 parts of calcium carbonate, 1-2 parts of monocalcium phosphate, 1-2 parts of multivitamin for dogs, 0.3-0.5 parts of choline chloride, 0.3-0.5 parts of chlorine potassium chloride, 0.3-0.5 parts of sodium chloride, 9.5-12 parts of chicken fat, 1-2 parts of fish oil, 6-8 parts of dog pulp, 1-2 parts of dog powder, 1.21-3.35 parts of additives, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, and 0.5-1 parts of Lactobacillus delbrueckii lactis, wherein the weight ratio of Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis is 2:1:2:1.

[0052] The method for preparing the high-tolerance fermented food for pet dogs comprises the following steps:

[0053] (1) fermenting corn and soybean meal with 1 part of Lactobacillus casei Lc12 and 0.5 part of Liposaccharomyces staryi and enzymolysis to obtain a fermentation product;

[0054] (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives to obtain a first premix;

[0055] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, dog pulp, dog meal, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed, and then crushed and passed through a 0.8 mm sieve, and then expanded and dried to obtain a second premix;

[0056] (4) Mix chicken oil and fish oil and spray them onto the surface of the second premix, and then spray dog ​​pulp and dog powder in sequence. After cooling, the mixture is obtained.

[0057] Optionally, the specific fermentation method for obtaining the fermented product after the corn and soybean meal in step (1) are fermented and enzymatically hydrolyzed by 1 part of Lactobacillus casei Lc12 and 0.5 part of Starburst yeast is:

[0058] S1: cooking corn and soybean meal in 30-50 times water, and filtering to obtain residue and filtrate;

[0059] S2: Mix the culture medium and the filter residue in a weight ratio of 1:(4-5), then add the filtrate 2-3 times the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 25-35°C for 4-5 days, sterilize and filter to obtain the fermentation liquid;

[0060] S3: Add 0.5 parts of aminopeptidase to the fermentation broth, perform enzymolysis at 60°C and pH 8.0 for 1-3 hours, then add 0.5 parts of saccharifying enzyme, perform enzymolysis at 55°C and pH 6.0 for 2-3 hours, and inactivate the enzyme at high temperature to obtain the fermentation product.

[0061] Optionally, the bacterial culture medium includes 2-3 g of peptone, 1-2 g of white sugar, 1.5-2 g of yeast powder, 0.5-1 g of potassium dihydrogen phosphate, and 100-120 g of purified water. The mixture is stirred evenly and sterilized at 121° C. for 20 min before use.

[0062] Optionally, the mixing time of step (2) is 80-120 s, and the mixing time of step (3) is 100-140 s.

[0063] Optionally, the puffing temperature in step (3) is 90-130° C., and the bulk density after puffing is 370-390 g / L.

[0064] Optionally, the drying temperature in step (3) is 105-110° C., and the drying is performed until the moisture content is 5.5-7.0%.

[0065] Optionally, in step (4), the temperature of the chicken oil and fish oil sprayed is 45-50°C, the temperature of the dog pulp sprayed is 45-50°C, the temperature of the dog powder sprayed is room temperature, and the spraying time of the entire step S3 does not exceed 2 minutes.

[0066] Optionally, the cooling in step (4) is performed by cooling with air at normal temperature.

[0067] The bacteria species, additives and fermentation methods added to the pet dog food are the same as those in the pet cat food, and the functions of the above substances are also the same, so they will not be described in detail.

[0068] The beneficial effects of this application include but are not limited to:

[0069] 1. This application adds four strains of bacteria to pet cat and dog food and sets the optimal strain ratio. First, it can inhibit the growth of harmful bacteria and pathogenic bacteria and increase the number of beneficial bacteria; second, it can enhance the repair ability of intestinal mucosa and improve the development and function of the intestinal immune system of elderly cats and dogs; third, it can reduce intestinal permeability, alleviate intestinal inflammatory response, and improve the symptoms of pet food intolerance; fourth, the digestive enzymes and various metabolites produced during the growth of the strains promote the body's digestion and absorption of nutrients.

[0070] 2. This application adds oligofructose, oligomaltose, glutamine, lactic acid and citric acid as additives to pet cat and dog food, which can inhibit inflammatory response, increase the growth and repair of intestinal mucosal cells, promote the growth of beneficial bacteria, inhibit the reproduction of harmful bacteria, and restore liver and intestine homeostasis.

[0071] 3. Since cats and dogs in old age will inevitably experience natural weight loss, the pet cat and dog food of the present application can delay intestinal degeneration of cats and dogs in old age, thereby reducing their natural weight loss rate, improving the internal health status of pet cats and dogs in old age, and reducing the occurrence of old age diseases.

[0072] 4. The present application uses fermentation of corn and soybean meal to improve the digestibility of dry matter, crude protein and organic matter of elderly pet cats and dogs. Enzymatic hydrolysis after fermentation can also increase the number of beneficial bacteria and reduce the number of harmful bacteria, further improving the tolerance of elderly cats and dogs. DETAILED DESCRIPTION

[0073] The present application is described in detail below with reference to embodiments, but the present application is not limited to these embodiments.

[0074] Unless otherwise specified, the raw materials in the examples and comparative examples of the present application were purchased through commercial channels. Among them, Lactobacillus casei Lc12, Bacillus coagulans, and Lactobacillus rhamnosus GG were purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd., and Lactobacillus delbrueckii subsp. lactis was purchased from Shaanxi Chenming Biotechnology Co., Ltd. Chicken meal was purchased from Shandong Luhai Biotechnology Co., Ltd., anchovy meal was purchased from Jinan Xinweita Science and Trade Co., Ltd., corn was purchased from farmers, corn protein meal was purchased from Hangzhou Muzi Agricultural Technology Co., Ltd., duck hemoglobulin powder was purchased from Weifang Purun Biotechnology Co., Ltd., sweet potato granules were purchased from Qingdao Cornell Pet Products Co., Ltd., rice was purchased from Jiangsu Hope Rice Co., Ltd., cassava starch was purchased from Qingdao Zhongtai Hechuang International Trade Co., Ltd., soybean meal was purchased from Shandong Shouhao International Trade Co., Ltd., beet meal was purchased from Shandong Shouhao International Trade Co., Ltd., cellulose was purchased from Germany Ruidenhaier (Shanghai) Fiber Trading Co., Ltd., taurine was purchased from Qianjiang Yongan Pharmaceutical Co., Ltd., calcium carbonate was purchased from Yichang Dengcheng Calcium Carbonate Development Co., Ltd., calcium dihydrogen phosphate was purchased from Weifang Limin Feed Co., Ltd., multivitamin was purchased from DSM Vitamins (Shandong) Co., Ltd., choline chloride was purchased from Shandong Jujia Biotechnology Co., Ltd. Company, potassium chloride was purchased from Chengdu Shuxing Feed Co., Ltd., chicken oil was purchased from Renxian Wangdi Animal Oil Co., Ltd., fish oil was purchased from Shandong Garden Fish Oil Technology Co., Ltd., cat pulp and dog pulp were purchased from Debifu Pet Food (Chuzhou) Co., Ltd., cat powder and dog powder were purchased from Jiangsu Yichong Biotechnology Co., Ltd., glutamine was purchased from Anhui Huaheng Biotechnology Co., Ltd., tyrosine was purchased from Anhui Huaheng Biotechnology Co., Ltd., oligofructose was purchased from Bowling Biotechnology Co., Ltd., isomaltooligosaccharide was purchased from Bowling Biotechnology Co., Ltd., yeast cell wall was purchased from Shandong Aikosente Biotechnology Co., Ltd., betaine was purchased from Shandong Zhongwei Biotechnology Co., Ltd., sodium butyrate was purchased from Jiangxi Tianjia Bioengineering Co., Ltd., bile acid was purchased from Shandong Longchang Animal Health Products Co., Ltd., lactic acid was purchased from Henan Jindan Lactic Acid Technology Co., Ltd., and citric acid was purchased from Weifang Yingxuan Industrial Co., Ltd. The above-mentioned multi-dimensional and multi-mineral components contain vitamin A, vitamin D3, vitamin E, vitamin K3, vitamin B1, vitamin B2, vitamin B6, vitamin B12, D-biotin, D-pantothenic acid, niacinamide, iron, copper, and manganese. Cat pulp and dog pulp are taste enhancers, and the main ingredients are paste-like substances formed by enzymatic hydrolysis of chicken and its by-products. Cat powder and dog powder are also a kind of seasoning, and the main ingredients include chicken liver, deep-sea fish, brewer's yeast, protease, sodium chloride and other substances.

[0075] Unless otherwise specified, the methods used in the embodiments of the present application are conventional methods in the prior art.

[0076] Example 1

[0077] The present embodiment relates to a high-tolerance fermented food for pet cats and a preparation method thereof. The food comprises, by weight: 30-32 parts of chicken meal, 5-8 parts of anchovy meal, 16-20 parts of corn, 3-6 parts of corn gluten meal; 20-24 parts of rice, 8-10 parts of cassava starch, 5-8 parts of soybean meal, 3-5 parts of beet meal, 2-4 parts of cellulose, 0.3-0.5 parts of taurine, 0.2-0.4 parts of calcium carbonate, 1-2 parts of multivitamin, 0.5-0.8 parts of choline chloride, 0.2-0.4 parts of potassium chloride, 9.5-12 parts of chicken fat, 1.5-2 parts of fish oil, 5 -7 parts of cat pulp, 1-2 parts of cat powder, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, 0.5-1 parts of Lactobacillus delbrueckii lactis, 0.1-0.3 parts of glutamine, 0.1-0.2 parts of tyrosine, 0.1-0.2 parts of fructooligosaccharides, 0.5-1.0 parts of isomaltooligosaccharides, 0.1-0.5 parts of yeast cell wall, 0.1-0.5 parts of betaine, 0.05-0.2 parts of sodium butyrate, 0.05-0.1 parts of bile acid, 0.01-0.05 parts of lactic acid and 0.1-0.3 parts of citric acid.

[0078] The viable count of the Lactobacillus casei Lc12 is 5×10 8 -10×10 8 cfu / g, and the viable count of Bacillus coagulans was 3×10 8 -8×10 8 cfu / g, and the viable bacterial count of the rhamnosus lactobacillus GG was 5×10 8 -10×10 8 cfu / g, and the viable count of the Lactobacillus delbrueckii subspecies lactis was 5×10 8 -10×10 8 cfu / g.

[0079] The preparation method of the high-tolerance fermented food for pet cats comprises the following steps:

[0080] (1) fermenting corn and soybean meal with 1 part of Lactobacillus casei Lc12 and 0.5 part of Liposaccharomyces staryi and enzymolysis to obtain a fermentation product;

[0081] (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives to obtain a first premix;

[0082] (3) The fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, cat pulp, cat powder, corn, soybean meal, and Lactobacillus casei Lc12 are then added to the first premix, and then crushed and passed through a 0.8 mm sieve, and then expanded and dried to obtain a second premix;

[0083] (4) Mix chicken oil and fish oil and spray them onto the surface of the second premix, and then spray cat paste and cat powder in sequence. After cooling, the mixture is obtained.

[0084] According to the above ratio and preparation method, cat food 1#-14# and comparative cat food D1#-D3# were prepared. The specific preparation is as follows:

[0085] Cat food 1#

[0086] Cat food 1# includes: 30 parts chicken meal, 5 parts anchovy meal, 20 parts corn, 3 parts corn gluten meal; 20 parts rice, 10 parts cassava starch, 5 parts soybean meal, 5 parts beet meal, 4 parts cellulose, 0.5 parts taurine, 0.2 parts calcium carbonate, 1 part multivitamin, 0.5 parts choline chloride, 0.4 parts potassium chloride, 9.5 parts chicken fat, 2 parts fish oil, 7 parts cat pulp, 2 parts cat powder, 3 parts Lactobacillus casei Lc12 (the number of live bacteria is 5×10 8 cfu / g), 1 Bacillus coagulans (viable count 3×10 8 cfu / g), 2 parts of Lactobacillus rhamnosus GG (viable count 5×10 8 cfu / g), 0.5 Lactobacillus delbrueckii subsp. lactis (5×10 8 cfu / g), 0.1 part glutamine, 0.2 part tyrosine, 0.2 part fructooligosaccharide, 1.0 part isomaltooligosaccharide, 0.5 part yeast cell wall, 0.1 part betaine, 0.05 part sodium butyrate, 0.1 part bile acid, 0.05 part lactic acid and 0.3 part citric acid.

[0087] The preparation method of cat food 1# is:

[0088] (1) S1: Boil corn and soybean meal in 50 times the amount of water, and filter to obtain residue and filtrate;

[0089] S2: Mix the culture medium and the filter residue in a weight ratio of 1:4, then add the filtrate twice the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 35°C for 4 days, sterilize and filter to obtain the fermentation liquid, wherein the culture medium includes 3g of peptone, 2g of white sugar, 2g of yeast powder, 1g of potassium dihydrogen phosphate, and 120g of purified water, stir evenly, sterilize at 121°C for 20 min before use;

[0090] S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 3 hours, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 2 hours, and obtaining a fermentation product after high-temperature enzyme inactivation;

[0091] (2) premixing the multivitamin, choline chloride, potassium chloride, calcium carbonate and additives for 120 seconds to obtain a first premix;

[0092] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, cat pulp, cat powder, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed for 140 seconds, then crushed and passed through a 0.8 mm sieve, and then puffed at 90° C., the bulk density after puffing is in the range of 370-390 g / L, and then dried at 110° C. to a moisture content of 7.0% to obtain a second premix;

[0093] (4) Mix the chicken oil and fish oil, heat to 45°C and spray on the surface of the second premix. Then add cat slurry and heat to 45°C for spraying. Finally, mix cat powder at room temperature and cool with air at room temperature to obtain the product.

[0094] Cat food 2#

[0095] Cat food 2# includes: 32 parts chicken meal, 8 parts anchovy meal, 16 parts corn, 6 parts corn gluten meal; 24 parts rice, 8 parts cassava starch, 8 parts soybean meal, 3 parts beet pulp, 2 parts cellulose, 0.3 parts taurine, 0.4 parts calcium carbonate, 2 parts multivitamin, 0.8 parts choline chloride, 0.2 parts potassium chloride, 12 parts chicken fat, 1.5 parts fish oil, 5 parts cat pulp, 1 part cat powder, 2 parts Lactobacillus casei Lc12 (live bacteria count 10×10 8 cfu / g), 2 parts of Bacillus coagulans (the number of viable bacteria was 8×10 8 cfu / g), 1 Lactobacillus rhamnosus GG (viable count 10×10 8 cfu / g), 1 Lactobacillus delbrueckii subsp. lactis (10×10 8 cfu / g), 0.3 parts of glutamine, 0.1 parts of tyrosine, 0.1 parts of fructooligosaccharides, 0.5 parts of isomaltooligosaccharides, 0.1 parts of yeast cell walls, 0.5 parts of betaine, 0.2 parts of sodium butyrate, 0.05 parts of bile acid, 0.01 parts of lactic acid and 0.1 parts of citric acid.

[0096] The preparation method of cat food 2# is:

[0097] (1) S1: Boil corn and soybean meal in 30 times the volume of water, and filter to obtain residue and filtrate;

[0098] S2: Mix the culture medium and the filter residue in a weight ratio of 1:5, then add the filtrate 3 times the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 25°C for 5 days, sterilize and filter to obtain the fermentation liquid, wherein the culture medium includes 2g of peptone, 1g of white sugar, 1.5g of yeast powder, 0.5g of potassium dihydrogen phosphate, and 100g of purified water, stir evenly, sterilize at 121°C for 20 min before use;

[0099] S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 1 hour, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 3 hours, and obtaining a fermentation product after high-temperature enzyme inactivation;

[0100] (2) premixing the multivitamin, choline chloride, potassium chloride, calcium carbonate and additives for 80 seconds to obtain a first premix;

[0101] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, cat pulp, cat powder, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed for 100 seconds, then crushed and passed through a 0.8 mm sieve, and then puffed at 130° C., and the bulk density after puffing is in the range of 370-390 g / L, and then dried at 105° C. to a moisture content of 5.5% to obtain a second premix;

[0102] (4) Mix the chicken oil and fish oil, heat to 50°C and spray on the surface of the second premix. Then add cat slurry and heat to 50°C for spraying. Finally, mix cat powder at room temperature and cool with air at room temperature to obtain the product.

[0103] Cat food 3#

[0104] Cat food 3# includes: 32 parts chicken meal, 6 parts anchovy meal, 18 parts corn, 5 parts corn gluten meal; 22 parts rice, 9 parts cassava starch, 6 parts soybean meal, 4 parts beet meal, 3 parts cellulose, 0.4 parts taurine, 0.3 parts calcium carbonate, 2 parts multivitamin, 0.5 parts choline chloride, 0.3 parts potassium chloride, 10 parts chicken fat, 2 parts fish oil, 6 parts cat pulp, 2 parts cat powder, 2 parts Lactobacillus casei Lc12 (the number of live bacteria is 9×10 8 cfu / g), 1 Bacillus coagulans (viable count 8×10 8 cfu / g), 2 parts of Lactobacillus rhamnosus GG (viable count 9×10 8 cfu / g), 1 Lactobacillus delbrueckii subsp. lactis (8×10 8 cfu / g), 0.3 parts of glutamine, 0.2 parts of tyrosine, 0.2 parts of fructooligosaccharides, 1.0 parts of isomaltooligosaccharides, 0.4 parts of yeast cell walls, 0.5 parts of betaine, 0.1 parts of sodium butyrate, 0.1 parts of bile acid, 0.05 parts of lactic acid and 0.25 parts of citric acid.

[0105] The preparation method of cat food 3# is:

[0106] (1) S1: Boil corn and soybean meal in 40 times the volume of water, and filter to obtain residue and filtrate;

[0107] S2: Mix the culture medium and the filter residue in a weight ratio of 1:5, then add 2.5 times the weight of the filter residue to obtain a fermented product after sterilization, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 30°C for 5 days, and filter to obtain a fermentation broth after sterilization, wherein the culture medium includes 2g of peptone, 1.5g of white sugar, 1.5g of yeast powder, 0.8g of potassium dihydrogen phosphate, and 110g of purified water, stir evenly, and sterilize at 121°C for 20 min before use;

[0108] S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 2 hours, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 3 hours, and obtaining a fermentation product after high-temperature enzyme inactivation;

[0109] (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives for 100 seconds to obtain a first premix;

[0110] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, cat pulp, cat powder, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed for 120 seconds, then crushed and passed through a 0.8 mm sieve, and then puffed at 120° C., and the bulk density after puffing is in the range of 370-390 g / L, and then dried at 105° C. to a moisture content of 6.0% to obtain a second premix;

[0111] (4) Mix the chicken oil and fish oil, heat to 45°C and spray on the surface of the second premix. Then add cat slurry and heat to 45°C for spraying. Finally, mix cat powder at room temperature and cool with air at room temperature to obtain the product.

[0112] Cat food 4#

[0113] The difference between this cat food and cat food 3# is that the added amount of glutamine is 0.55 parts, and the rest is the same as cat food 3#.

[0114] Cat food 5#

[0115] The difference between this cat food and cat food 3# is that the oligofructose is 0.1 parts, and the rest is the same as cat food 3#.

[0116] Cat food 6#

[0117] The difference between this cat food and cat food 3# is that the betaine content is 0.1 parts, and the rest is the same as cat food 3#.

[0118] Cat food 7#

[0119] The difference between this cat food and cat food 3# is that the content of this cat food is 2 parts of Bacillus coagulans and 0.5 parts of Lactobacillus delbrueckii subsp. lactis, and the rest is the same as cat food 3#.

[0120] Cat food 8#

[0121] The difference between the cat food and cat food 3# is that Starburst yeast is not added in S2 of step (1) of the preparation method, and the rest is the same as cat food 3#.

[0122] Cat food 9#

[0123] The difference between this cat food and cat food 3# is that step S2 and step S3 of the preparation method (1) are reversed, specifically:

[0124] S2: Mix the culture medium and the filter residue in a weight ratio of 1:5, then add the filtrate 2.5 times the weight of the filter residue, sterilize to obtain the product to be hydrolyzed, add 0.5 parts of aminopeptidase to the product to be hydrolyzed, hydrolyze at 60°C and pH 8.0 for 2 hours, then add 0.5 parts of saccharifying enzyme, hydrolyze at 55°C and pH 6.0 for 3 hours to obtain the product to be fermented, wherein the culture medium includes 2g of peptone, 1.5g of white sugar, 1.5g of yeast powder, 0.8g of potassium dihydrogen phosphate, and 110g of purified water, stir evenly, sterilize at 121°C for 20 min before use;

[0125] S3: Inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast into the fermented material, ferment at 30°C for 5 days, sterilize and filter to obtain the fermented material. The rest is the same as cat food 3#.

[0126] Cat food 10#

[0127] The difference between the cat food and cat food 3# is that no filtrate is added in S2 of step (1) of the preparation method, and the culture medium and the filter residue are directly mixed and sterilized to obtain the fermented product, and the rest is the same as cat food 3#.

[0128] Cat food 11#

[0129] The difference between the present cat food and cat food 3# is that in step (1) S3 of the preparation method, alkaline protease is used to replace aminopeptidase, and the rest is the same as cat food 3#.

[0130] Cat food 12#

[0131] The difference between the present cat food and cat food 3# is that in step (1) of the preparation method, in S3, saccharifying enzyme is first used for primary enzymolysis, and then aminopeptidase is used for secondary enzymolysis, and the enzymolysis conditions of aminopeptidase and saccharifying enzyme are the same as those of cat food 3#, and the rest is the same as cat food 3#.

[0132] Cat food 13#

[0133] The difference between the cat food and cat food 3# is that the aminopeptidase in S3 of step (1) of the preparation method is 1.0 part, and the rest is the same as cat food 3#.

[0134] Cat food 14#

[0135] The difference between the cat food and cat food 3# is that the saccharifying enzyme in S3 of step (1) of the preparation method is 0.2 parts, and the rest is the same as cat food 3#.

[0136] Compare cat food D1#

[0137] The difference between this comparative cat food and cat food 3# is that Bacillus subtilis is used to replace Bacillus coagulans, and the rest is the same as cat food 3#.

[0138] Compare cat food D2#

[0139] The difference between this comparative cat food and cat food 3# is that Lactobacillus paracasei is used to replace Lactobacillus rhamnosus GG, and the rest is the same as cat food 3#.

[0140] Compare cat food D3#

[0141] The difference between this comparative cat food and cat food 3# is that Bifidobacterium lactis is used to replace Lactobacillus delbrueckii subsp. lactis, and the rest is the same as cat food 3#.

[0142] Test Example 1

[0143] This test example receives and integrates the feedback results of 170 elderly pet cats as evaluation indicators. Elderly pet cats refer to cats older than 8 years old. The 170 elderly pet cats are randomly divided into 17 groups, each with 10 cats, and they are fed with the cat food prepared in the above Example 1 for a 28-day feeding period. Each cat is raised in a single pen in a pet cage, and necessary immunization and deworming treatment are performed before the test. The cat litter is changed every day, the enclosure is cleaned, and the pet enclosure is kept clean.

[0144] 1. Feeding performance measurement

[0145] During the test period, the daily feeding amount, residual feed amount, water feeding amount and residual water amount were recorded, and the daily feed intake and water drinking amount were calculated. The daily average feed intake and water drinking amount were calculated based on the daily feed intake and water drinking amount. The apparent digestibility of crude protein, crude fat and organic matter and the dry matter digestibility were also calculated. The test results are shown in Table 1, where the number of diarrhea times in Table 1 refers to the total number of diarrhea times for cats in each group during the entire test period.

[0146] Table 1

[0147]

[0148] According to the data in Table 1, the cat food prepared by the present application can increase the amount of food intake and water intake, proving that the pet cat is in a good state of feeding, and improves the digestibility of crude protein, organic matter and dry matter of the pet cat, and reduces the number of diarrhea, proving that the cat food can improve the tolerance of elderly cats. Cat food 8#-14# changes the fermentation method of corn and soybean meal. It can be seen from the data that different fermentation methods affect the feed intake and nutrient absorption of pet cats, and cat food 8# and 9# can also cause diarrhea in elderly cats, proving that the fermentation and enzymolysis method of the present application and the strains used for fermentation can further improve the tolerance of elderly cats.

[0149] 2. Determination of intestinal health, including stool scoring, stool content examination, and determination of fecal ammonia nitrogen and pH. The test results are shown in Table 3.

[0150] Fecal score: During the experimental period, the fecal score was recorded before feeding every day, and the fecal score was based on the Royal Cat Fecal Score Standard.

[0151] Fecal content examination: During the test period, the feces were examined every two days to observe the discharge of cat hair in the feces, and the cat hair content in the feces was scored according to the standards in Table 2.

[0152] Table 2

[0153]

[0154] Determination of fecal ammonia nitrogen and pH: Take 2g of feces and add 10mL of distilled water to completely dissolve it, then filter it with gauze, and take the filtrate for determination. The concentration of ammonia nitrogen in feces is detected by indophenol blue-spectrophotometry. Take 5g of fresh feces sample, dilute and mix it with 10mL of distilled water, and measure its pH with E301CF pH acidity meter.

[0155] Table 3

[0156]

[0157] According to the data in Table 3, the pet cat food of the present application can promote the excretion of hair from feces and reduce the content of ammonia nitrogen in feces, which means that the pet cat food helps elderly pet cats to establish a healthy intestinal system. It not only has a high tolerance for the pet cat food, but also can help pet cats to better tolerate other foods and improve their own functions.

[0158] Intestinal microbiome analysis of cat food 3# and comparison cat food D1#-D3#: At 28 days, 5 cats in each group were sampled for feces, and the samples were sent to Beijing Novogene Technology Co., Ltd. for 16S rDNA metagenomic analysis using high-throughput sequencing technology. In order to observe the differences in species richness between the groups, the top ten species in abundance were selected to calculate the relative abundance at the intestinal microbial phylum level, and the results are shown in Table 4.

[0159] Table 4

[0160]

[0161] According to the data in Table 4, the dominant flora in the feces of cat food group 3# is Firmicutes, Bacteroidetes and Fusobacteria, and these three phyla account for about 80% of the total bacteria. The dominant flora of the comparative cat food group D1# is Firmicutes, Bacteroidetes and Actinobacteria, and these three phyla account for about 84% of the total bacteria. The dominant flora of the comparative cat food D2# and comparative cat food D3# groups are Firmicutes, Bacteroidetes, Actinobacteria, Fusobacteria and Proteobacteria, and these five phyla account for about 85% of the total bacteria.

[0162] 3. Sensory performance evaluation, the scores of each item are shown in Table 5, and the scores of the following items are 0-10 points, among which:

[0163] Sensory score 10: The product has a loose texture, a full appearance, good gloss, and a strong smell after opening the bag. The pet has a clear appetite and can maintain a good appetite after eating it for several consecutive days.

[0164] Mental state 10 points: The cat is in good mental state during the continuous period of eating the product, and does not have abnormal mental state such as sudden loss of emotional control or depression;

[0165] Nasal mucosa condition 10 points: The nasal mucosa is moist and presents the normal state before consuming this product and maintains a healthy physical state during the continuous cycle of consuming this product (except for cases where the nasal mucosa is abnormal due to disease);

[0166] Urine and stool status: 10 points: The odor and status of urine and stool are normal, or the odor of stool has improved; no abnormal status occurs during the continuous cycle of consuming this product;

[0167] Skin and hair condition: 10 points: healthy skin, no inflammation, redness, etc.; hair is smooth and shiny, with no obvious hair loss;

[0168] Overall growth: 10 points: The weight is consistent with the growth of an elderly cat of this age; the growth curve is normal.

[0169] Table 5

[0170]

[0171] According to the data in Table 5, pets that eat this pet cat food can grow normally and are in good mental state. Replacing it with other strains will reduce the appetite of pet cats, thereby affecting the state and overall growth of pet cats.

[0172] Example 2

[0173] The present embodiment relates to a high-tolerance fermented food for pet dogs and a preparation method thereof. The food comprises, by weight: 19-22 parts of chicken meal, 24-27 parts of corn, 6-8 parts of corn gluten meal, 27-30 parts of rice, 1-2 parts of duck blood globulin powder, 8-10 parts of sweet potato granules, 6-8 parts of soybean meal, 3-5 parts of beet meal, 2-4 parts of cellulose, 0.7-1.0 parts of calcium carbonate, 1-2 parts of calcium dihydrogen phosphate, 1-2 parts of multivitamin, 0.3-0.5 parts of choline chloride, 0.3-0.5 parts of potassium chloride, 0.3-0.5 parts of sodium chloride, 9.5-12 parts of chicken fat, 1-2 parts of fish oil, 6-8 parts of dog pulp, 1-2 parts of dog powder, 1.21-3.35 parts of additives, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, 0.5-1 parts of Lactobacillus delbrueckii lactis, 0.1-0.3 parts of glutamine, 0.1-0.2 parts of tyrosine, 0.1-0.2 parts of fructooligosaccharides, 0.5-1.0 parts of isomaltooligosaccharides, 0.1-0.5 parts of yeast cell wall, 0.1-0.5 parts of betaine, 0.05-0.2 parts of sodium butyrate, 0.05-0.1 parts of bile acid, 0.01-0.05 parts of lactic acid and 0.1-0.3 parts of citric acid.

[0174] The viable count of the Lactobacillus casei Lc12 is 5×10 8 -10×10 8 cfu / g, and the viable bacterial count of the Bacillus coagulans was 3×10 8 -8×10 8 cfu / g, and the viable bacterial count of the rhamnosus lactobacillus GG was 5×10 8 -10×10 8 cfu / g, and the viable count of the Lactobacillus delbrueckii subspecies lactis was 5×10 8 -10×10 8 cfu / g.

[0175] The method for preparing the high-tolerance fermented food for pet dogs comprises the following steps:

[0176] (1) fermenting corn and soybean meal with 1 part of Lactobacillus casei Lc12 and 0.5 part of Liposaccharomyces staryi and enzymolysis to obtain a fermentation product;

[0177] (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives to obtain a first premix;

[0178] (3) adding the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, dog pulp, dog meal, corn, soybean meal, and Lactobacillus casei Lc12 to the first premix, and then crushing and passing through a 0.8 mm sieve, and then expanding and drying to obtain a second premix;

[0179] (4) Mix chicken oil and fish oil and spray them onto the surface of the second premix, and then spray dog ​​pulp and dog powder in sequence. After cooling, the mixture is obtained.

[0180] According to the above ratio and preparation method, dog food 1#-14# and comparative dog food D1#-D3# were prepared. The specific preparation is as follows:

[0181] Dog Food 1#

[0182] Dog food 1# includes: 19 parts chicken meal, 24 parts corn, 8 parts corn gluten meal, 30 parts rice, 2 parts duck blood globulin powder, 10 parts sweet potato granules, 8 parts soybean meal, 3 parts beet meal, 4 parts cellulose, 0.7 parts calcium carbonate, 2 parts monocalcium phosphate, 1 part multivitamin, 0.3 parts choline chloride, 0.5 parts potassium chloride, 0.3 parts sodium chloride, 12 parts chicken fat, 1 part fish oil, 8 parts dog pulp, 1 part dog meal, 3 parts Lactobacillus casei Lc12 (the number of live bacteria is 5×10 8 cfu / g), 1 Bacillus coagulans (viable count 3×10 8 cfu / g), 2 parts of Lactobacillus rhamnosus GG (viable count 5×10 8 cfu / g), 0.5 Lactobacillus delbrueckii subsp. lactis (5×10 8 cfu / g), 0.1 part glutamine, 0.2 part tyrosine, 0.2 part fructooligosaccharide, 1.0 part isomaltooligosaccharide, 0.5 part yeast cell wall, 0.1 part betaine, 0.05 part sodium butyrate, 0.1 part bile acid, 0.05 part lactic acid and 0.3 part citric acid.

[0183] The preparation method of dog food 1# is:

[0184] (1) S1: Boil corn and soybean meal in 30 times the volume of water, and filter to obtain residue and filtrate;

[0185] S2: Mix the culture medium and the filter residue in a weight ratio of 1:4, then add the filtrate 3 times the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 35°C for 4 days, sterilize and filter to obtain the fermentation liquid, wherein the culture medium includes 3g of peptone, 2g of white sugar, 2g of yeast powder, 1g of potassium dihydrogen phosphate, and 120g of purified water, stir evenly, sterilize at 121°C for 20 min before use;

[0186] S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 3 hours, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 2 hours, and obtaining a fermentation product after high-temperature enzyme inactivation;

[0187] (2) premixing the multivitamin, choline chloride, potassium chloride, calcium carbonate and additives for 120 seconds to obtain a first premix;

[0188] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, dog pulp, dog meal, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed for 140 seconds, then crushed and passed through a 0.8 mm sieve, and then puffed at 130° C., and the bulk density after puffing is in the range of 370-390 g / L, and then dried at 110° C. to a moisture content of 7.0% to obtain a second premix;

[0189] (4) Mix the chicken oil and fish oil, heat them to 45°C, and spray them on the surface of the second premix. Then add the dog pulp, heat it to 45°C, and spray it. Finally, mix the dog powder at room temperature and cool it with air at room temperature.

[0190] Dog Food 2#

[0191] Dog food 2# includes: 22 parts chicken meal, 27 parts corn, 6 parts corn gluten meal, 27 parts rice, 1 part duck blood globulin powder, 8 parts sweet potato granules, 6 parts soybean meal, 5 parts beet meal, 2 parts cellulose, 1.0 part calcium carbonate, 1 part calcium dihydrogen phosphate, 2 parts multivitamin, 0.5 part choline chloride, 0.3 part potassium chloride, 0.5 part sodium chloride, 9.5 parts chicken fat, 2 parts fish oil, 6 parts dog pulp, 2 parts dog meal, 2 parts Lactobacillus casei Lc12 (live bacteria count 10×10 8 cfu / g), 2 parts of Bacillus coagulans (the number of viable bacteria was 8×10 8 cfu / g), 1 Lactobacillus rhamnosus GG (viable count 10×10 8 cfu / g), 1 Lactobacillus delbrueckii subsp. lactis (10×10 8cfu / g), 0.3 parts of glutamine, 0.1 parts of tyrosine, 0.1 parts of fructooligosaccharides, 0.5 parts of isomaltooligosaccharides, 0.1 parts of yeast cell walls, 0.5 parts of betaine, 0.2 parts of sodium butyrate, 0.05 parts of bile acid, 0.01 parts of lactic acid and 0.1 parts of citric acid.

[0192] The preparation method of dog food 2# is:

[0193] (1) S1: Boil corn and soybean meal in 50 times the amount of water, and filter to obtain residue and filtrate;

[0194] S2: Mix the culture medium and the filter residue in a weight ratio of 1:5, then add the filtrate twice the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 25°C for 5 days, sterilize and filter to obtain the fermentation liquid, wherein the culture medium includes 2g of peptone, 1g of white sugar, 1.5g of yeast powder, 0.5g of potassium dihydrogen phosphate, and 100g of purified water, stir evenly, sterilize at 121°C for 20 min before use;

[0195] S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 1 hour, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 3 hours, and obtaining a fermentation product after high-temperature enzyme inactivation;

[0196] (2) premixing the multivitamin, choline chloride, potassium chloride, calcium carbonate and additives for 80 seconds to obtain a first premix;

[0197] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken oil, fish oil, dog pulp, dog meal, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed for 100 seconds, then crushed and passed through a 0.8 mm sieve, and then puffed at 90° C., and the bulk density after puffing is in the range of 370-390 g / L, and then dried at 105° C. to a moisture content of 5.5% to obtain a second premix;

[0198] (4) Mix the chicken oil and fish oil, heat them to 50°C, and spray them on the surface of the second premix. Then add the dog pulp, heat it to 50°C, and spray it. Finally, mix the dog powder at room temperature and cool it with air at room temperature.

[0199] Dog food 3#

[0200] Dog food 3# includes: 21 parts chicken meal, 25 parts corn, 7 parts corn gluten meal, 28 parts rice, 2 parts duck blood globulin powder, 8 parts sweet potato granules, 7 parts soybean meal, 4 parts beet meal, 3 parts cellulose, 1.0 parts calcium carbonate, 1.5 parts calcium dihydrogen phosphate, 1.5 parts multivitamin, 0.4 parts choline chloride, 0.4 parts potassium chloride, 0.4 parts sodium chloride, 11 parts chicken fat, 2 parts fish oil, 7 parts dog pulp, 1.5 parts dog meal, 2 parts Lactobacillus casei Lc12 (the number of live bacteria is 9×10 8 cfu / g), 1 Bacillus coagulans (viable count 8×10 8 cfu / g), 2 parts of Lactobacillus rhamnosus GG (viable count 9×10 8 cfu / g), 1 Lactobacillus delbrueckii subsp. lactis (8×10 8 cfu / g), 0.3 parts of glutamine, 0.2 parts of tyrosine, 0.2 parts of fructooligosaccharides, 1.0 parts of isomaltooligosaccharides, 0.4 parts of yeast cell walls, 0.5 parts of betaine, 0.1 parts of sodium butyrate, 0.1 parts of bile acid, 0.05 parts of lactic acid and 0.25 parts of citric acid.

[0201] The preparation method of dog food 3# is:

[0202] (1) S1: Boil corn and soybean meal in 40 times the volume of water, and filter to obtain residue and filtrate;

[0203] S2: Mix the culture medium and the filter residue in a weight ratio of 1:4, then add 2.5 times the weight of the filter residue filtrate, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 30°C for 5 days, sterilize and filter to obtain the fermentation liquid, wherein the culture medium includes 2g of peptone, 1.5g of white sugar, 1.5g of yeast powder, 0.8g of potassium dihydrogen phosphate, and 110g of purified water, stir evenly, sterilize at 121°C for 20 min before use;

[0204] S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 2 hours, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 3 hours, and obtaining a fermentation product after high-temperature enzyme inactivation;

[0205] (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives for 100 seconds to obtain a first premix;

[0206] (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken oil, fish oil, dog pulp, dog meal, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed for 120 seconds, then crushed and passed through a 0.8 mm sieve, and then puffed at 120° C., and the bulk density after puffing is in the range of 370-390 g / L, and then dried at 105° C. to a moisture content of 6.0% to obtain a second premix;

[0207] (4) Mix the chicken oil and fish oil, heat them to 45°C, and spray them on the surface of the second premix. Then add the dog pulp, heat it to 45°C, and spray it. Finally, mix the dog powder at room temperature and cool it with air at room temperature.

[0208] Dog Food 4#

[0209] The difference between this dog food and dog food 3# is that the added amount of glutamine is 0.1 parts, and the rest is the same as dog food 3#.

[0210] Dog food 5#

[0211] The difference between this dog food and dog food 3# is that the content of isomaltooligosaccharide is 0.5 parts, and the rest is the same as dog food 3#.

[0212] Dog Food 6#

[0213] The difference between this dog food and dog food 3# is that the lactic acid is 0.01 parts, and the rest is the same as dog food 3#.

[0214] Dog Food 7#

[0215] The difference between this dog food and dog food 3# is that the Lactobacillus casei Lc12 is 3 parts and the Lactobacillus rhamnosus GG is 1 part, and the rest is the same as dog food 3#.

[0216] Dog Food 8#

[0217] The difference between the dog food and dog food 3# is that Lactobacillus casei Lc12 is not added in S2 of step (1) of the preparation method, and the rest is the same as dog food 3#.

[0218] Dog Food 9#

[0219] The difference between the dog food and dog food 3# is that step S2 and step S3 of the preparation method (1) are reversed, specifically:

[0220] S2: Mix the culture medium and the filter residue in a weight ratio of 1:4, then add the filtrate 2.5 times the weight of the filter residue, sterilize to obtain the product to be hydrolyzed, add 0.5 parts of aminopeptidase to the product to be hydrolyzed, hydrolyze at 60°C and pH 8.0 for 2 hours, then add 0.5 parts of saccharifying enzyme, hydrolyze at 55°C and pH 6.0 for 3 hours to obtain the product to be fermented, wherein the culture medium includes 2g of peptone, 1.5g of white sugar, 1.5g of yeast powder, 0.8g of potassium dihydrogen phosphate, and 110g of purified water, stir evenly, and sterilize at 121°C for 20 min before use;

[0221] S3: Inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast into the fermented material, ferment at 30°C for 5 days, sterilize and filter to obtain the fermented material. The rest is the same as dog food 3#.

[0222] Dog food 10#

[0223] The difference between the dog food and dog food 3# is that the amount of filtrate added in S2 of step (1) of the preparation method is 5 times the weight of the filter residue, and the culture medium and the filter residue are directly mixed and sterilized to obtain the fermented product, and the rest is the same as dog food 3#.

[0224] Dog Food 11#

[0225] The difference between the dog food and dog food 3# is that in step (1) S3 of the preparation method, acid protease is used to replace aminopeptidase, and the rest is the same as dog food 3#.

[0226] Dog food 12#

[0227] The difference between the dog food and dog food 3# is that in step (1) of the preparation method, in S3, saccharifying enzyme is first used for primary enzymolysis, and then aminopeptidase is used for secondary enzymolysis, and the enzymolysis conditions of aminopeptidase and saccharifying enzyme are the same as those of dog food 3#, and the rest is the same as dog food 3#.

[0228] Dog food 13#

[0229] The difference between the dog food and dog food 3# is that the aminopeptidase in S3 of step (1) of the preparation method is 0.2 parts, and the rest is the same as dog food 3#.

[0230] Dog food 14#

[0231] The difference between the dog food and dog food 3# is that the saccharifying enzyme in S3 of step (1) of the preparation method is 1.0 part, and the rest is the same as dog food 3#.

[0232] Comparison Dog Food D1#

[0233] The difference between this comparative dog food and dog food 3# is that Bacillus subtilis is used to replace Bacillus coagulans, and the rest is the same as dog food 3#.

[0234] Comparison Dog Food D2#

[0235] The difference between this comparative dog food and dog food 3# is that Lactobacillus paracasei is used to replace Lactobacillus rhamnosus GG, and the rest is the same as dog food 3#.

[0236] Comparison Dog Food D3#

[0237] The difference between the comparative dog food and dog food 3# is that Bifidobacterium lactis is used to replace Lactobacillus delbrueckii subsp. lactis, and the rest is the same as dog food 3#.

[0238] Test Example 2

[0239] This test example receives and integrates the feedback results of 170 elderly pet dogs as evaluation indicators. Elderly pet dogs refer to dogs older than 8 years old. The 170 elderly pet dogs are randomly divided into 17 groups, each with 10 dogs. They are fed with the dog food prepared in the above Example 2 for 28 days. Each dog is raised in a single pen in a pet cage, and necessary immunization and deworming treatment are performed before the test. The pens are cleaned and maintained clean every day.

[0240] 1. Feeding performance measurement

[0241] During the test period, the daily feeding amount, leftover feed amount, water feeding amount and leftover water amount were recorded, and the daily feed intake and water drinking amount were calculated. The daily average feed intake and water drinking amount were calculated based on the daily feed intake and water drinking amount. The apparent digestibility of crude protein, crude fat and organic matter and the dry matter digestibility were also calculated. The test results are shown in Table 6, where the number of diarrhea times in Table 6 refers to the total number of diarrhea times for dogs in each group during the entire test period.

[0242] Table 6

[0243]

[0244] According to the data in Table 6, the pet dog food prepared by the present application can increase the food intake and water intake of elderly pet dogs, improve the absorption of nutrients by elderly pet dogs, reduce diarrhea, and improve the tolerance of elderly pet dogs. Dog foods 8#-14# change the fermentation method of corn and soybean meal. It can be seen from the data that different fermentation methods affect the food intake and nutrient absorption of pet dogs, and dog foods 8# and 9# can also cause diarrhea in elderly pet dogs, proving that the fermentation-first-enzyme-hydrolysis method and the strains used in the fermentation of the present application can further improve the tolerance of elderly pet dogs.

[0245] 2. Determination of intestinal health, including examination of stool contents and determination of fecal ammonia nitrogen. The test results are shown in Table 7.

[0246] Fecal content examination: During the test period, the feces were examined every two days to observe the discharge of dog hair in the feces, and the dog hair content in the feces was scored according to the standards in Table 7.

[0247] Table 7

[0248]

[0249] Determination of fecal ammonia nitrogen: Take 2g of feces and add 10mL of distilled water to completely dissolve it, then filter it with gauze and take the filtrate for determination. The concentration of ammonia nitrogen in feces is detected by indophenol blue-spectrophotometry.

[0250] Table 8

[0251]

[0252] According to the data in Table 8, the pet dog food of the present application can promote the excretion of hair from feces and reduce the content of ammonia nitrogen in feces, which means that the pet dog food helps elderly pet dogs establish a healthy intestinal system. It not only has a high tolerance to the pet dog food, but also can help pet dogs better tolerate other foods even when ingested, thereby improving the functions of elderly pet dogs themselves.

[0253] 3. Sensory performance evaluation, the scores of each item are shown in Table 9, and the scores of the following items are 0-10 points, among which:

[0254] Sensory score 10: The product has a loose texture, a full appearance, good gloss, and a strong smell after opening the bag. The pet has a clear appetite and can maintain a good appetite after eating it for several consecutive days;

[0255] Mental state 10 points: The dog is in good mental state during the continuous period of eating the product, and does not have abnormal mental state such as sudden loss of emotional control or depression;

[0256] Nasal mucosa condition 10 points: The nasal mucosa is moist and presents the normal state before consuming this product and maintains a healthy physical state during the continuous cycle of consuming this product (except for cases where the nasal mucosa is abnormal due to disease);

[0257] Urine and stool status: 10 points: The odor and status of urine and stool are normal, or the odor of stool has improved; no abnormal status occurs during the continuous cycle of consuming this product;

[0258] Skin and hair condition: 10 points: healthy skin, no inflammation, redness, etc.; hair is smooth and shiny, with no obvious hair loss;

[0259] Overall growth: 10 points: The weight is consistent with the growth of elderly dogs of this age; the growth curve is normal.

[0260] Table 9

[0261]

[0262] According to the data in Table 9, elderly pet dogs that eat this pet dog food can meet the normal growth curve. The changes in the fermentation methods of corn and soybean meal affect the absorption rate of nutrients by pets, thereby indirectly affecting the appetite and mental state of pet dogs.

[0263] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A high-tolerance fermented food for pet cats, characterized in that: By weight, it includes 30-32 parts chicken meal, 16-20 parts corn, 3-6 parts corn gluten meal, 5-8 parts soybean meal, 3-5 parts beet meal, 2-4 parts cellulose, 0.2-0.4 parts calcium carbonate, 1-2 parts multivitamin, 0.5-0.8 parts choline chloride, 0.2-0.4 parts potassium chloride, 9.5-12 parts chicken fat, 1.5-2 parts fish oil, 5-8 parts anchovy meal, 20-24 parts rice, 8-10 parts Tapioca starch, 0.3-0.5 parts of taurine, 5-7 parts of cat pulp, 1-2 parts of cat powder, 1.21-3.35 parts of additives, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, and 0.5-1 parts of Lactobacillus delbrueckii lactis, wherein the weight ratio of Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis is 2:1:2:1; The method for preparing high-tolerance fermented food for pet cats comprises the following steps: (1) S1: Boil corn and soybean meal in 30-50 times water, and filter to obtain residue and filtrate; S2: Mix the culture medium and the filter residue in a weight ratio of 1:(4-5), then add the filtrate 2-3 times the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 25-35°C for 4-5 days, sterilize and filter to obtain the fermentation liquid; S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 1-3 hours, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 2-3 hours, and inactivating the enzyme at high temperature to obtain a fermentation product; (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives to obtain a first premix; (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, cat pulp, cat powder, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed, and then crushed and passed through a 0.8 mm sieve, and then expanded and dried to obtain a second premix; (4) Mix chicken oil and fish oil and spray them onto the surface of the second premix, and then spray cat paste and cat powder in sequence. After cooling, the mixture is obtained.

2. The high-tolerance fermented food for pet cats according to claim 1, characterized in that: The additives include: 0.1-0.3 parts of glutamine, 0.1-0.2 parts of tyrosine, 0.1-0.2 parts of fructo-oligosaccharide, 0.5-1.0 parts of isomaltooligosaccharide, 0.1-0.5 parts of yeast cell wall, 0.1-0.5 parts of betaine, 0.05-0.2 parts of sodium butyrate, 0.05-0.1 parts of bile acid, 0.01-0.05 parts of lactic acid, and 0.1-0.3 parts of citric acid.

3. The high-tolerance fermented food for pet cats according to claim 2, characterized in that: The weight ratio of fructooligosaccharide to isomaltooligosaccharide in the above additives is 1:5, and the weight ratio of betaine, lactic acid and citric acid is 10:1:

5.

4. The high-tolerance fermented food for pet cats according to claim 1, characterized in that: The viable bacterial count of Lactobacillus casei Lc12 is 5×10 8 -10×10 8 cfu / g, and the viable count of Bacillus coagulans was 3×10 8 -8×10 8 cfu / g, and the viable bacterial count of the rhamnosus lactobacillus GG was 5×10 8 -10×10 8 cfu / g, and the viable count of the Lactobacillus delbrueckii subspecies lactis was 5×10 8 -10×10 8 cfu / g.

5. A high-tolerance fermented food for pet dogs, characterized in that: By weight, it includes 19-22 parts of chicken meal, 24-27 parts of corn, 6-8 parts of corn gluten meal, 6-8 parts of soybean meal, 3-5 parts of beet pulp, 2-4 parts of cellulose, 0.7-1.0 parts of calcium carbonate, 1-2 parts of multivitamin, 0.3-0.5 parts of choline chloride, 0.3-0.5 parts of potassium chloride, 9.5-12 parts of chicken fat and 1-2 parts of fish oil, 27-30 parts of rice, 1-2 parts of duck blood globulin powder, 8-10 parts of sweet potato granules, 1-2 parts of calcium dihydrogen phosphate, 0.3-0.5 parts of sodium chloride, 6-8 parts of dog pulp and 1-2 parts of dog powder, 1.21-3.35 parts of additives, 2-3 parts of Lactobacillus casei Lc12, 1-2 parts of Bacillus coagulans, 1-2 parts of Lactobacillus rhamnosus GG, 0.5-1 parts of Lactobacillus delbrueckii lactis, wherein the weight ratio of Lactobacillus casei Lc12, Bacillus coagulans, Lactobacillus rhamnosus GG and Lactobacillus delbrueckii lactis is 2:1:2:1; The method for preparing high-tolerance fermented food for pet dogs comprises the following steps: (1) S1: Boil corn and soybean meal in 30-50 times water, and filter to obtain residue and filtrate; S2: Mix the culture medium and the filter residue in a weight ratio of 1:(4-5), then add the filtrate 2-3 times the weight of the filter residue, sterilize to obtain the fermented product, then cool and inoculate 1 part of Lactobacillus casei Lc12 and 0.5 part of Starch yeast, ferment at 25-35°C for 4-5 days, sterilize and filter to obtain the fermentation liquid; S3: adding 0.5 parts of aminopeptidase to the fermentation broth, performing enzymolysis at 60°C and pH 8.0 for 1-3 hours, then adding 0.5 parts of saccharifying enzyme, performing enzymolysis at 55°C and pH 6.0 for 2-3 hours, and inactivating the enzyme at high temperature to obtain a fermentation product; (2) premixing multivitamin, choline chloride, potassium chloride, calcium carbonate and additives to obtain a first premix; (3) Then, the fermented product, the remaining Lactobacillus casei Lc12, and the raw materials other than chicken fat, fish oil, dog pulp, dog meal, corn, soybean meal, and Lactobacillus casei Lc12 are added to the first premix and mixed, and then crushed and passed through a 0.8 mm sieve, and then expanded and dried to obtain a second premix; (4) Mix chicken oil and fish oil and spray them onto the surface of the second premix, and then spray dog ​​pulp and dog powder in sequence. After cooling, the mixture is obtained.

6. The high-tolerance fermented food for pet dogs according to claim 5, characterized in that: The additives include: 0.1-0.3 parts of glutamine, 0.1-0.2 parts of tyrosine, 0.1-0.2 parts of fructo-oligosaccharide, 0.5-1.0 parts of isomaltooligosaccharide, 0.1-0.5 parts of yeast cell wall, 0.1-0.5 parts of betaine, 0.05-0.2 parts of sodium butyrate, 0.05-0.1 parts of bile acid, 0.01-0.05 parts of lactic acid, and 0.1-0.3 parts of citric acid.

7. The high-tolerance fermented food for pet dogs according to claim 6, characterized in that: The weight ratio of fructooligosaccharide to isomaltooligosaccharide in the above additives is 1:5, and the weight ratio of betaine, lactic acid and citric acid is 10:1:

5.

8. The high-tolerance fermented food for pet dogs according to claim 5, characterized in that: The viable bacterial count of Lactobacillus casei Lc12 is 5×10 8 -10×10 8 cfu / g, and the viable count of Bacillus coagulans was 3×10 8 -8×10 8 cfu / g, and the viable bacterial count of the rhamnosus lactobacillus GG was 5×10 8 -10×10 8 cfu / g, and the viable count of the Lactobacillus delbrueckii subspecies lactis was 5×10 8 -10×10 8 cfu / g.

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