Dynamic detection method, device and computer-readable storage medium for leukocyte detection

Abstract

The present invention relates to the field of blood analysis, and discloses a dynamic detection method for white blood cell detection, which enables a first detection mode or a second detection mode: when the second detection mode is enabled, a preset first statistic is supplied to a detection channel and a white blood cell detection result is obtained to determine whether the blood sample is a low-value white blood cell sample: if not, a preset second statistic is supplied to the detection channel for detection; if so, a third statistic is calculated based on a preset white blood cell statistical particle number threshold, and the calculated third statistic is supplied to the detection channel for detection, and a second white blood cell detection result is quickly obtained. The present invention also proposes a computer-readable storage medium and a sample detection device.

Description

Technical Field

[0001] The present invention belongs to the technical field of in vitro diagnosis, and specifically relates to a dynamic detection method, device and computer-readable storage medium for leukocyte detection. Background Art

[0002] In modern medical systems, it is often necessary to analyze particles in the blood to obtain various parameters, such as scatter plots of various particles or specific particles, and to count the number of particles (such as cells). In the prior art, the statistics for the classification of white blood cells are usually measured according to a fixed volume, such as by pushing a fixed volume sample through a syringe. In other words, the volume of the syringe for pushing the sample is fixed. For samples with low white blood cell values, the statistics will be small, which will have a certain impact on the accuracy of the classification. Therefore, the method of analyzing particles such as blood cells according to a fixed volume sample in the prior art has defects. For low-value (concentration value) particle samples, the number of samples actually analyzed will be very small, resulting in reduced accuracy of classification measurement.

[0003] In actual testing, both low-value WBC and high-value WBC have important clinical significance, and it is very important to accurately measure their results. Therefore, it is necessary to propose a method that can accurately measure low-value white blood cells without affecting the test efficiency.

[0004] In the prior art, the main focus is on optimizing the measurement method for low-value WBC samples. For example, a subsample for total particle count and particle classification measurement is prepared. The sample is pushed according to a fixed amount first. After the total particle count is measured by an optical channel, the total particle count is compared with the threshold. The comparison result is then fed back to the injection unit, and the sample is further pushed according to the second push volume (the second volume is larger than the first push volume) for classification counting to increase the statistics and improve accuracy. This method requires combining the two channels of WNB and DIFF to perform WBC multiplication judgment and implementation, involving two channels, with a complex design and easily interfered by the abnormality of the WNB channel, which leads to misjudgment. At the same time, it can only process low WBC values, and does not process medium and high value samples, which is not conducive to improving measurement efficiency.

[0005] For another example, during the white blood cell measurement process, the white blood cell count of the sample detected within an initial period of detection time is used to determine whether the blood sample is a low-value white blood cell sample; if so, the sample pushing time of the power device is extended from the first sample pushing time to the preset second sample pushing time. However, the doubling time of this method can only be a fixed second sample pushing time, and does not take into account the differences in WBC values ​​of various samples, making it difficult to adapt to complex testing scenarios. At the same time, it can only process low-value WBC samples, and does not process medium and high-value samples, which is not conducive to improving measurement efficiency. Summary of the invention

[0006] In view of this, an object of the present invention is to provide a dynamic detection method, device and computer-readable storage medium for leukocyte detection, which is conducive to improving the detection accuracy of leukocyte samples and maximizing the detection efficiency.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] The present invention first proposes a dynamic detection method for leukocyte detection, the method comprising:

[0009] Enable the first detection mode or the second detection mode;

[0010] When the first detection mode is enabled:

[0011] A first sample to be tested is prepared by using a blood sample, and the first sample to be tested is supplied to a detection channel for detection according to a preset fifth statistic, and a detection signal of the first sample to be tested is obtained, wherein the detection signal of the first sample to be tested is at least used to obtain a first white blood cell detection result of the blood sample, and the first white blood cell detection result is output;

[0012] When the second detection mode is enabled:

[0013] A second sample to be tested is prepared by using a blood sample, and the second sample to be tested is supplied to a detection channel by using a power device, and the second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained, and the detection signal of the second sample to be tested is at least used to obtain a white blood cell detection result of the blood sample, and based on the white blood cell detection result of the second sample to be tested of the first statistic, it is determined whether the blood sample is a low-value white blood cell sample;

[0014] When the blood sample is judged to be a non-low-value white blood cell sample, the second sample to be tested is supplied to the detection channel for testing according to the preset second statistic, and the second white blood cell test result is output; when the blood sample is judged to be a low-value white blood cell sample, the third statistic of the required second sample to be tested is calculated using the preset white blood cell statistical particle number threshold, the second sample to be tested is pushed into the detection channel according to the calculated third statistic, and the third white blood cell test result is output.

[0015] In the second detection mode, by identifying whether the current sample is a low-value white blood cell sample in advance before the first statistic of the normal detection, and then performing different white blood cell tests based on the identification results, the accuracy of the white blood cell detection can be more efficiently improved. At the same time, by using the preset white blood cell statistical particle number threshold to calculate the third statistic of the required second test sample, it can be ensured that the total number of white blood cell particles counted is always not lower than the preset white blood cell statistical particle number threshold, thereby fundamentally ensuring the accuracy of the test results.

[0016] Among them, the preset white blood cell statistical particle number threshold is a set lower limit value of the white blood cell particle number. When it is lower than the lower limit value, the number of white blood cell particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the white blood cell detection result is not high. Therefore, it is necessary to ensure that the number of white blood cell particles counted in the detection channel is not lower than the set lower limit value of the white blood cell statistical particle number. In the present invention, the third statistic of the second test sample required is calculated using the preset white blood cell statistical particle number threshold, which can effectively ensure that a sufficient number of white blood cell particles are counted, obtain a sufficient detection signal, and ultimately ensure the detection precision and accuracy of the white blood cell detection result. Specifically, the number of white blood cell particles in the test sample of the third statistic is greater than or equal to the preset white blood cell statistical particle number threshold.

[0017] Further, the detection channel is an impedance detection channel or an optical detection channel. Further, the detection channel of the first detection mode is an impedance detection channel, and the detection channel of the second detection mode is also an impedance detection channel; or, the detection channel of the first detection mode is an impedance detection channel, and the detection channel of the second detection mode is an optical detection channel; or, the detection channel of the first detection mode is an optical detection channel, and the detection channel of the second detection mode is an impedance detection channel; or, the detection channel of the first detection mode is an optical detection channel, and the detection channel of the second detection mode is also an optical detection channel. Further, the detection channel of the first detection mode may include both an impedance detection channel and an optical detection channel; the detection channel of the second detection mode may include both an impedance detection channel and an optical detection channel.

[0018] Furthermore, the first sample to be tested and the second sample to be tested are the same or different. Specifically, for example, when the detection channel of the first detection mode and the detection channel of the second detection mode are the same, the first sample to be tested and the second sample to be tested may be the same or different. For another example, when the detection channel of the first detection mode and the detection channel of the second detection mode are different, the first sample to be tested and the second sample to be tested are generally different samples; in some special cases, the same sample may also be used for detection in two different detection channels.

[0019] Furthermore, when the second detection mode is enabled, the method for determining whether the blood sample is a low-value white blood cell sample is as follows: the white blood cell concentration is calculated based on the number of white blood cell particles, and whether the white blood cell concentration is lower than a preset concentration threshold: if so, the blood sample is a low-value white blood cell sample; if not, the blood sample is not a low-value white blood cell sample.

[0020] Furthermore, the theoretical value of the third statistic is It is calculated by the following formula: ; Theoretical value of the third statistic Round up to get the actual value of the third statistic : ;in: The particle count threshold for the preset white blood cell count; The supply quantity of the sample to be tested reaches the preset first statistic The number of white blood cell particles is counted at this time; “[]” is the rounding function; is the minimum value of the statistic.

[0021] Among them, the third statistic is the actual value of the third statistic Or the theoretical value of the third statistic ; The second sample to be tested is calculated according to the actual value of the third statistic Or the theoretical value of the third statistic Push it into the detection channel and output the third white blood cell detection result.

[0022] In this way, it can ensure that the number of leukocyte particles in the sample to be tested of the third statistic is greater than or equal to the preset leukocyte statistical particle number threshold while not excessively increasing the statistic of the sample to be tested, thereby effectively improving the detection efficiency.

[0023] Furthermore, it is preferred to calculate the actual value of the third statistic obtained by calculating the second test sample. Push it into the detection channel and output the third white blood cell detection result. The minimum measurement value of the statistic is introduced in the calculation process , which takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second sample to be tested supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0024] Optionally, the third statistic is greater than or equal to a theoretical value of the third statistic Any value of .

[0025] Further, when the blood sample is determined to be a low-value white blood cell sample, if the third statistic is greater than a preset fourth statistic, the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and a fourth white blood cell detection result is output.

[0026] For some special low-value white blood cell samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of white blood cell particles. Therefore, in order to cope with this scenario, a fourth statistic is set. For example, the fourth statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be set by the operator). When the third statistic of the required second sample to be tested calculated using the preset white blood cell statistical particle number threshold is greater than the preset fourth statistic, the second sample to be tested is supplied to the detection channel according to the preset fourth statistic for detection, and the fourth white blood cell detection result is output. At this time, although the white blood cell particle count obtained by counting is less than the preset white blood cell statistical particle count threshold, the white blood cell particle count obtained by counting is still the maximum white blood cell particle count that can be obtained by counting the second sample to be tested currently prepared, so as to maximize the detection accuracy of the fourth white blood cell detection result.

[0027] Optionally, when the blood sample is judged to be a low-value white blood cell sample, if the third statistic is greater than a preset fourth statistic, a prompt message is output, or the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and the fourth white blood cell detection result and prompt message are output.

[0028] Optionally, when the blood sample is determined to be a low-leukocyte sample, if the third statistic is greater than a preset fourth statistic, the blood sample is retested.

[0029] Furthermore, a third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

[0030] Furthermore, before executing the white blood cell test of the blood sample, a start instruction of the first detection mode or the second detection mode is triggered by a user or automatically.

[0031] Furthermore, the start instruction of the first detection mode or the second detection mode is triggered by the user. If the user triggers the start instruction of the first detection mode, the first detection mode is enabled to detect the blood sample; if the user triggers the start instruction of the second detection mode, the second detection mode is enabled to detect the blood sample.

[0032] Furthermore, before enabling the first detection mode or the second detection mode, it also includes: obtaining a click instruction of a visual operation interface, or a trigger instruction of a control panel; the visual operation interface displays a first detection mode option and a second detection mode option; the control panel is provided with a first detection mode button and a second detection mode button; wherein, enabling the first detection mode or the second detection mode includes: when the click instruction is a click instruction for the first detection mode option, or when the trigger instruction is a trigger instruction for the first detection mode button, enabling the first detection mode; when the click instruction is a click instruction for the second detection mode option, or when the trigger instruction is a trigger instruction for the second detection mode button, enabling the second detection mode.

[0033] Furthermore, the start instruction of the first detection mode or the second detection mode is automatically triggered. When the current blood sample executes the second detection mode to complete the detection and is a low-value white blood cell sample, the start instruction of the second detection mode is automatically triggered when the white blood cell detection of the subsequent blood sample is executed; otherwise, the start instruction of the first detection mode is automatically triggered.

[0034] The present invention also proposes a computer-readable storage medium, characterized in that it includes a program, and the program can be executed by a processor to implement the dynamic detection method for white blood cell detection as described above.

[0035] The present invention also proposes a sample detection device, comprising: a sampling and distribution module, a reagent supply unit, a mixing chamber, a detection module, a power device and a mode setting unit; the sampling and distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling and distribution module and the reagent provided by the reagent supply unit to prepare a sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; a mode setting unit is used to enable a first mode, or to enable a second mode; the detection device also includes a processor, which is used to execute the program in a computer-readable storage medium as described above.

[0036] The present invention also proposes a dynamic detection method for leukocyte detection, the method comprising:

[0037] A second sample to be tested is prepared by using a blood sample, and the second sample to be tested is supplied to a detection channel by using a power device, and the second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained, and the detection signal of the second sample to be tested is at least used to obtain a white blood cell detection result of the blood sample, and based on the white blood cell detection result of the second sample to be tested of the first statistic, it is determined whether the blood sample is a low-value white blood cell sample;

[0038] When the blood sample is judged to be a non-low-value white blood cell sample, the second sample to be tested is supplied to the detection channel for testing according to the preset second statistic, and the second white blood cell test result is output; when the blood sample is judged to be a low-value white blood cell sample, the third statistic of the required second sample to be tested is calculated using the preset white blood cell statistical particle number threshold, the second sample to be tested is pushed into the detection channel according to the calculated third statistic, and the third white blood cell test result is output.

[0039] The number of leukocyte particles in the second sample to be tested of the third statistic of the required second sample to be tested calculated using the preset leukocyte statistical particle number threshold is greater than or equal to the preset leukocyte statistical particle number threshold.

[0040] Furthermore, the theoretical value of the third statistic is It is calculated by the following formula: ; Theoretical value of the third statistic Round up to get the actual value of the third statistic : ;in: The particle count threshold for the preset white blood cell count; The supply quantity of the sample to be tested reaches the preset first statistic The number of white blood cell particles is counted at this time; “[]” is the rounding function; is the minimum value of the statistic.

[0041] Among them, the third statistic is the actual value of the third statistic Or the theoretical value of the third statistic ; The second sample to be tested is calculated according to the actual value of the third statistic Or the theoretical value of the third statistic Push it into the detection channel and output the third white blood cell detection result.

[0042] In this way, it can ensure that the number of leukocyte particles in the sample to be tested of the third statistic is greater than or equal to the preset leukocyte statistical particle number threshold while not excessively increasing the statistic of the sample to be tested, thereby effectively improving the detection efficiency.

[0043] Optionally, the third statistic is greater than or equal to a theoretical value of the third statistic Any value of .

[0044] Furthermore, it is preferred to calculate the actual value of the third statistic obtained by calculating the second test sample. Push it into the detection channel and output the third white blood cell detection result. The minimum measurement value of the statistic is introduced in the calculation process , which takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second sample to be tested supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0045] Further, when the blood sample is determined to be a low-value white blood cell sample, if the third statistic is greater than a preset fourth statistic, the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and a fourth white blood cell detection result is output.

[0046] Further, when the blood sample is judged to be a low-value white blood cell sample, if the third statistic is greater than the preset fourth statistic, a prompt message is output, or the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and the fourth white blood cell detection result and prompt message are output.

[0047] Furthermore, when the blood sample is determined to be a low-leukocyte sample, if the third statistic is greater than a preset fourth statistic, the blood sample is retested.

[0048] Furthermore, a third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

[0049] Further, the detection channel is an impedance detection channel and / or an optical detection channel.

[0050] The present invention also provides a computer-readable storage medium, comprising a program, wherein the program can be executed by a processor to implement the dynamic detection method for leukocyte detection as described above.

[0051] The present invention also proposes a sample detection device, comprising: a sampling and distribution module, a reagent supply unit, a mixing chamber, a detection module and a power device; the sampling and distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling and distribution module and the reagent provided by the reagent supply unit to prepare a sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described above.

[0052] The beneficial effects of the present invention are:

[0053] The dynamic detection method for white blood cell detection of the present invention can enable a first detection mode and a second detection mode: when the second detection mode is enabled, the second sample to be tested is supplied to the detection channel according to a preset first statistic and a white blood cell detection result is obtained to determine whether the blood sample is a low-value white blood cell sample: if it is not a low-value white blood cell sample, the second sample to be tested is continued to be supplied to the detection channel according to the preset second statistic for detection to quickly obtain the first white blood cell detection result; if it is a low-value white blood cell sample, a third statistic is calculated based on a preset white blood cell statistical particle number threshold, and the second sample to be tested is continued to be supplied to the detection channel according to the calculated third statistic for detection, and the third white blood cell detection result is quickly obtained; the detection accuracy can be guaranteed and the detection efficiency is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] In order to make the purpose, technical solution and beneficial effects of the present invention clearer, the present invention provides the following drawings for illustration:

[0055] Figure 1 This is a flow chart of a first embodiment of the method for dynamic detection of leukocytes of the present invention;

[0056] Figure 2 This is a flow chart of a second embodiment of the method for dynamic detection of leukocytes of the present invention;

[0057] Figure 3 This is a flow chart of a third embodiment of the method for dynamic detection of leukocytes of the present invention;

[0058] Figure 4 This is a flow chart of the fourth embodiment of the leukocyte dynamic detection method of the present invention. DETAILED DESCRIPTION

[0059] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0060] like Figure 1 As shown, the leukocyte dynamic detection method of this embodiment includes the following steps.

[0061] S100: Activate the first detection mode or activate the second detection mode.

[0062] If the first detection mode is enabled, a first sample to be tested is prepared (ie, step S110 is executed).

[0063] Then, step S111 is performed: supplying the first sample to be tested of the fifth statistic to the detection channel, obtaining a detection signal of the first sample to be tested of the fifth statistic through the detection device, wherein the detection signal of the first sample to be tested of the fifth statistic is at least used to obtain a first white blood cell detection result of the blood sample.

[0064] S112: Output the first white blood cell detection result.

[0065] If the second detection mode is enabled, a second sample to be tested is prepared (ie, step S120 is executed).

[0066] Then, step S121 is performed: supplying the second sample to be tested with the first statistic to the detection channel, and obtaining a detection signal of the second sample to be tested by the detection device, wherein the detection signal of the second sample to be tested is at least used to obtain a first leukocyte detection result of the blood sample.

[0067] S122: Determine whether the blood sample is a low-value leukocyte sample. Specifically, based on the detection signal of the second test sample of the first statistic, calculate the leukocyte concentration, and determine whether the leukocyte concentration is lower than a preset concentration threshold. If so, the blood sample is a low-value leukocyte sample; if not, the blood sample is not a low-value leukocyte sample.

[0068] When it is determined that the blood sample is not a low-value white blood cell sample, step S123 is executed: a second sample to be tested of a second statistic is supplied to the detection channel (specifically, by adding the original first statistic to the second statistic). A detection signal of the second sample to be tested of the second statistic is obtained by a detection device, wherein the detection signal of the second sample to be tested of the second statistic is at least used to obtain the first white blood cell detection result of the blood sample.

[0069] S124: Output the second white blood cell detection result.

[0070] When the blood sample is determined to be a low-value white blood cell sample, step S125 is executed: a second sample to be tested of the third statistic is supplied to the detection channel (specifically, this is achieved by increasing the original first statistic to the third statistic). A detection signal of the second sample to be tested of the third statistic is obtained by a detection device, wherein the detection signal of the second sample to be tested of the third statistic is at least used to obtain a third white blood cell detection result of the blood sample. The third statistic of the required second sample to be tested is calculated using a preset white blood cell statistical particle number threshold.

[0071] S126: Output the third white blood cell detection result.

[0072] Among them, the preset white blood cell statistical particle number threshold is a set lower limit value of the white blood cell particle number. When it is lower than the lower limit value, the number of white blood cell particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the white blood cell detection result is not high. Therefore, it is necessary to ensure that the number of white blood cell particles counted in the detection channel is not lower than the set lower limit value of the white blood cell statistical particle number. In the present invention, the third statistic of the second test sample required is calculated using the preset white blood cell statistical particle number threshold, which can effectively ensure that a sufficient number of white blood cell particles are counted, obtain a sufficient detection signal, and ultimately ensure the detection precision and accuracy of the white blood cell detection result. Specifically, the number of white blood cell particles in the test sample of the third statistic is greater than or equal to the preset white blood cell statistical particle number threshold.

[0073] In this embodiment, the third statistic is obtained by calculation. Specifically, the theoretical value of the third statistic is It is calculated by the following formula: ; Theoretical value of the third statistic Round up to get the actual value of the third statistic : ;in: The particle count threshold for the preset white blood cell count; The supply quantity of the sample to be tested reaches the preset first statistic The number of white blood cell particles is counted at this time; “[]” is the rounding function; is the minimum value of the statistic.

[0074] In this embodiment, the third statistic is the actual value of the third statistic ; The second sample to be tested is calculated according to the actual value of the third statistic Push it to the detection channel and output the third white blood cell detection result. The calculation formula introduces the rounding function "[]" and the minimum measurement value of the statistic. , which can effectively convert the theoretical value of the third statistic obtained by theoretical calculation into Converting it into a third statistic that is actually operable helps the analytical instrument to supply the third statistic to the sample to be tested, and takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second sample to be tested supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0075] In an optional implementation, the third statistic is a theoretical value of the third statistic ; The second sample to be tested is calculated according to the theoretical value of the third statistic Push it into the detection channel and output the third white blood cell detection result.

[0076] In this way, in the second detection mode, when a low-value white blood cell sample appears, by using the preset white blood cell statistical particle number threshold to calculate the third statistic of the required second test sample, it can be ensured that the total number of white blood cell particles counted is always not lower than the preset white blood cell statistical particle number threshold, thereby fundamentally ensuring the accuracy of the test results.

[0077] In some other optional implementations, the third statistic may be greater than or equal to the theoretical value of the third statistic. Any value of .

[0078] Furthermore, in some application scenarios, for low-value white blood cell samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of white blood cell particles. Therefore, in order to cope with this scenario, a fourth statistic is set. For example, the fourth statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be manually set by the operator).

[0079] In the second detection mode, when the third statistic of the second test sample required for calculation by using the preset white blood cell statistical particle number threshold is greater than the preset fourth statistic, the second test sample with the fourth statistic is supplied to the detection channel, and the fourth platelet detection result is output; in this way, the preset fourth statistic is supplied to the detection channel for detection, which can ensure that the maximum white blood cell particle number statistic is obtained under the current detection disclosure, so as to improve the detection accuracy of the fourth white blood cell detection result.

[0080] Another implementation method, such as Figure 2As shown, when the judgment result of step S122 is yes (i.e., the blood sample is judged to be a low-value white blood cell sample), step S127 is executed: judging whether the third statistic is greater than the fourth statistic. When the third statistic is greater than the preset fourth statistic, step S128 is executed: supplying the second sample to be tested of the fourth statistic to the detection channel; S129: outputting the fourth white blood cell detection result. When the third statistic is less than the preset fourth statistic, step S125 is executed: supplying the second sample to be tested of the third statistic to the detection channel; S126: outputting the third white blood cell detection result.

[0081] In an optional implementation, when the judgment result of step S127 is yes (i.e., the third statistic is greater than the fourth statistic), if the test sample is still supplied to the detection channel according to the fourth statistic, the obtained white blood cell detection result may be inaccurate, and therefore, a prompt message may be directly output. Optionally, in this case, although the test sample is still supplied to the detection channel according to the fourth statistic (because the fourth statistic is less than the third statistic of the second test sample required for calculating the preset white blood cell counting particle number threshold, a sufficient number of white blood cell particles cannot be counted in the detection channel), the obtained white blood cell detection result may be inaccurate, but the white blood cell detection result can be used as a reference result, and therefore, the second test sample can be selected to be supplied to the detection channel for detection according to the fourth statistic, and the fourth white blood cell detection result and prompt message can be output for reference by the operator.

[0082] Optionally, the prompt information includes: abnormal result prompt, retest prompt, etc. The prompt information may also include corresponding test data information, such as the theoretical value of the second statistic, the third statistic, the dilution ratio of the current sample, the estimated maximum dilution ratio of retest, etc.

[0083] Further, in another embodiment, when the judgment result of step S127 is obtained as yes (that is, the third statistic is greater than the fourth statistic), a prompt message and / or a retest is output. When performing the retest, a third sample to be tested is prepared using a blood sample for retest, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested. In this way, the concentration of white blood cells in the third sample to be tested is greater than the concentration of white blood cells in the second sample to be tested, and the number of white blood cell particles obtained by statistics of the third sample to be tested with the same statistic is greater than the number of white blood cell particles obtained by statistics in the second sample to be tested, that is, the detection is performed using the third sample to be tested with a higher concentration, which can ensure that when the statistic of the third sample to be tested is less than the fourth statistic, the preset white blood cell statistical particle number threshold can be reached, so that the white blood cell detection result that meets the accuracy requirements can be obtained. Specifically, the detection channel is an impedance detection channel or an optical detection channel. The retest can be performed in the optical detection channel, or it can be completed in the impedance detection channel, preferably in the optical detection channel.

[0084] In some embodiments, the first sample to be tested and the second sample to be tested may be the same or different.

[0085] In other embodiments, the detection channel of the first detection mode and the detection channel of the second detection mode may be the same or different.

[0086] In some embodiments, before performing the white blood cell test of the blood sample, the start instruction of the first detection mode or the second detection mode can be triggered by the user or automatically.

[0087] When the start instruction of the first detection mode or the second detection mode is triggered by the user, if the user triggers the start instruction of the first detection mode, the first detection mode is enabled to detect the blood sample; if the user triggers the start instruction of the second detection mode, the second detection mode is enabled to detect the blood sample. Before enabling the first detection mode or the second detection mode, it also includes: obtaining a click instruction of the visual operation interface, or a trigger instruction of the control panel. The visual operation interface displays the first detection mode option and the second detection mode option; the control panel is provided with a first detection mode button and a second detection mode button. Specifically, the trigger operation when enabling the first detection mode includes: when the click instruction is a click instruction for the first detection mode option, or when the trigger instruction is a trigger instruction for the first detection mode button, the first detection mode is enabled. The trigger operation when enabling the second detection mode includes: when the click instruction is a click instruction for the second detection mode option, or when the trigger instruction is a trigger instruction for the second detection mode button, the second detection mode is enabled.

[0088] When the start instruction of the first detection mode or the second detection mode is automatically triggered, if the current blood sample executes the second detection mode to complete the detection and is a low-value white blood cell sample, the start instruction of the second detection mode is automatically triggered when the white blood cell detection of the next blood sample is executed; otherwise, the start instruction of the first detection mode is automatically triggered.

[0089] This embodiment also proposes a computer-readable storage medium, including a program, which can be executed by a processor to implement the white blood cell dynamic detection method described above in this embodiment.

[0090] This embodiment also proposes a sample detection device, including: a sampling distribution module, a reagent supply unit, a mixing chamber, a detection module, a power device and a mode setting unit. Specifically, the sampling distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling distribution module and the reagent provided by the reagent supply unit to prepare the sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; the mode setting unit is used to enable the first mode, or enable the second mode; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described above in this embodiment.

[0091] In this embodiment, the detection channel is an impedance detection channel or an optical detection channel.

[0092] In one embodiment, Figure 3 As shown, a specific scheme of pre-judging whether a blood sample is a low-value white blood cell sample before a normal white blood cell detection statistic is obtained, and then setting different low-value white blood cell sample detection methods according to the judgment results is shown.

[0093] Specifically, a method for dynamic detection of leukocytes comprises the following steps:

[0094] S220: preparing a second sample to be tested.

[0095] S221: supply the second sample to be tested of the first statistic to the detection channel, and obtain the detection signal of the second sample to be tested of the first statistic through the detection device, wherein the detection signal of the second sample to be tested of the first statistic is at least used to obtain the first white blood cell detection result of the blood sample.

[0096] S222: Determine whether the blood sample is a low-value leukocyte sample. Specifically, based on the detection signal of the second test sample of the first statistic, calculate the leukocyte concentration, and determine whether the leukocyte concentration is lower than a preset concentration threshold. If so, the blood sample is a low-value leukocyte sample; if not, the blood sample is not a low-value leukocyte sample.

[0097] When it is determined that the blood sample is not a low-value white blood cell sample, step S223 is executed: a second sample to be tested of a second statistic is supplied to the detection channel (specifically, by adding the original first statistic to the second statistic). A detection signal of the second sample to be tested of the second statistic is obtained by a detection device, wherein the detection signal of the second sample to be tested of the second statistic is at least used to obtain the first white blood cell detection result of the blood sample.

[0098] S224: Output the second white blood cell detection result.

[0099] When the blood sample is determined to be a low-value white blood cell sample, step S225 is executed: a second sample to be tested of the third statistic is supplied to the detection channel (specifically, this is achieved by increasing the original first statistic to the third statistic). A detection signal of the second sample to be tested of the third statistic is obtained by a detection device, wherein the detection signal of the second sample to be tested of the third statistic is at least used to obtain a third white blood cell detection result of the blood sample. The third statistic of the required second sample to be tested is calculated using a preset white blood cell statistical particle number threshold.

[0100] S226: Output the third white blood cell detection result.

[0101] Among them, the preset white blood cell statistical particle number threshold is a set lower limit value of the white blood cell particle number. When it is lower than the lower limit value, the number of white blood cell particles counted in the detection channel is insufficient, and the obtained detection signal is also insufficient, and there is a situation where the accuracy of the white blood cell detection result is not high. Therefore, it is necessary to ensure that the number of white blood cell particles counted in the detection channel is not lower than the set lower limit value of the white blood cell statistical particle number. In the present invention, the third statistic of the second test sample required is calculated using the preset white blood cell statistical particle number threshold, which can effectively ensure that a sufficient number of white blood cell particles are counted, obtain a sufficient detection signal, and ultimately ensure the detection precision and accuracy of the white blood cell detection result. Specifically, the number of white blood cell particles in the test sample of the third statistic is greater than or equal to the preset white blood cell statistical particle number threshold.

[0102] In this embodiment, the third statistic is obtained by calculation. Specifically, the third statistic is Theoretical value It is calculated by the following formula: ; Theoretical value of the third statistic Round up to get the actual value of the third statistic : ;in: The particle count threshold for the preset white blood cell count; The supply quantity of the sample to be tested reaches the preset first statistic The number of white blood cell particles is counted at this time; “[]” is the rounding function; is the minimum value of the statistic.

[0103] In this embodiment, the third statistic is the actual value of the third statistic ; The second sample to be tested is calculated according to the actual value of the third statistic Push it to the detection channel and output the third white blood cell detection result. The calculation formula introduces the rounding function "[]" and the minimum measurement value of the statistic. , which can effectively convert the theoretical value of the third statistic obtained by theoretical calculation into Converting it into a third statistic that is actually operable helps the analytical instrument to supply the third statistic to the sample to be tested, and takes into account the sample pushing accuracy of the power device, thereby ensuring that the statistics of the second sample to be tested supplied to the detection channel are more accurate, thereby ensuring the accuracy of subsequent detection results.

[0104] In an optional implementation, the third statistic is a theoretical value of the third statistic ; The second sample to be tested is calculated according to the theoretical value of the third statistic Push it into the detection channel and output the third white blood cell detection result.

[0105] In this way, when a low-value white blood cell sample appears, by using the preset white blood cell statistical particle number threshold to calculate the third statistic of the required second test sample, it can be ensured that the total number of white blood cell particles counted is always not lower than the preset white blood cell statistical particle number threshold, thereby fundamentally ensuring the accuracy of the test results.

[0106] In some other optional implementations, the third statistic may be greater than or equal to the theoretical value of the third statistic. Any value of .

[0107] Furthermore, in some application scenarios, for low-value white blood cell samples, it is possible that when the maximum statistic allowed by the detection device is supplied to the detection channel, it is still impossible to count a sufficient number of white blood cell particles. Therefore, in order to cope with this scenario, a fourth statistic is set. For example, the fourth statistic can be the maximum statistic allowed by the detection device, the maximum statistic of a single push of the syringe, or other statistic setting values ​​(which can be manually set by the operator).

[0108] When the third statistic of the second test sample required for calculation by using the preset white blood cell statistical particle number threshold is greater than the preset fourth statistic, the second test sample with the fourth statistic is supplied to the detection channel, and the fourth platelet test result is output; in this way, the preset fourth statistic is supplied to the detection channel for detection, which can ensure that the maximum white blood cell particle number statistic is obtained under the current detection disclosure, so as to improve the detection accuracy of the fourth white blood cell test result.

[0109] Another implementation method, such as Figure 4As shown, when the judgment result of step S222 is yes (i.e., the blood sample is judged to be a low-value white blood cell sample), step S227 is executed: judging whether the third statistic is greater than the fourth statistic. When the third statistic is greater than the preset fourth statistic, step S228 is executed: supplying the second sample to be tested of the fourth statistic to the detection channel; S229: outputting the fourth white blood cell detection result. When the third statistic is less than the preset fourth statistic, step S225 is executed: supplying the second sample to be tested of the third statistic to the detection channel; S226: outputting the third white blood cell detection result.

[0110] In an optional implementation, when the judgment result of step S227 is yes (i.e., the third statistic is greater than the fourth statistic), if the test sample is still supplied to the detection channel according to the fourth statistic, the obtained white blood cell detection result may be inaccurate, and therefore, a prompt message may be directly output. Optionally, in this case, although the test sample is still supplied to the detection channel according to the fourth statistic (because the fourth statistic is less than the third statistic of the second test sample required for calculating the preset white blood cell counting particle number threshold, a sufficient number of white blood cell particles cannot be counted in the detection channel), the obtained white blood cell detection result may be inaccurate, but the white blood cell detection result can be used as a reference result, and therefore, the second test sample can be selected to be supplied to the detection channel for detection according to the fourth statistic, and the fourth white blood cell detection result and prompt message can be output for reference by the operator.

[0111] Further, in another embodiment, when the judgment result of step S227 is obtained as yes (that is, the third statistic is greater than the fourth statistic), a prompt message and / or a re-test is output. When performing the re-test, a third sample to be tested is prepared using a blood sample for re-testing, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested. In this way, the concentration of white blood cells in the third sample to be tested is greater than the concentration of white blood cells in the second sample to be tested, and the number of white blood cell particles obtained by statistics of the third sample to be tested with the same statistic is greater than the number of white blood cell particles obtained by statistics in the second sample to be tested, that is, the detection is performed using the third sample to be tested with a higher concentration, which can ensure that when the statistic of the third sample to be tested is less than the fourth statistic, the preset white blood cell statistical particle number threshold can be reached, so that the white blood cell detection result that meets the accuracy requirements can be obtained. Specifically, the detection channel is an impedance detection channel or an optical detection channel. The re-test can be performed in the optical detection channel, or the re-test can be completed in the impedance detection channel, preferably the re-test is completed in the optical detection channel.

[0112] This embodiment also proposes a computer-readable storage medium, including a program, which can be executed by a processor to implement the dynamic detection method for white blood cell detection as described above in this embodiment.

[0113] The present embodiment also proposes a sample detection device, comprising: a sampling distribution module, a reagent supply unit, a mixing chamber, a detection module and a power device; the sampling distribution module is configured to collect blood samples and supply the blood samples at least partially to the mixing chamber; the reagent supply unit is used to supply reagents to the mixing chamber; the mixing chamber is used to receive the blood sample distributed by the sampling distribution module and the reagent provided by the reagent supply unit to prepare a sample to be tested; the detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the sample to be tested to the optical detection channel or the impedance detection channel; the detection device also includes a processor, the processor is used to execute the program in a computer-readable storage medium as described above in the present embodiment.

[0114] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A dynamic detection method for leukocyte detection, characterized in that: The method comprises: Enable the first detection mode or the second detection mode; When the first detection mode is enabled: A first sample to be tested is prepared by using a blood sample, and the first sample to be tested is supplied to a detection channel for detection according to a preset fifth statistic, and a detection signal of the first sample to be tested is obtained, wherein the detection signal of the first sample to be tested is at least used to obtain a first white blood cell detection result of the blood sample, and the first white blood cell detection result is output; When the second detection mode is enabled: A second sample to be tested is prepared by using a blood sample, and the second sample to be tested is supplied to a detection channel by using a power device, and the second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained, and the detection signal of the second sample to be tested is at least used to obtain a white blood cell detection result of the blood sample, and based on the white blood cell detection result of the second sample to be tested of the first statistic, it is determined whether the blood sample is a low-value white blood cell sample; When the blood sample is judged to be a non-low-value white blood cell sample, the second sample to be tested is supplied to the detection channel for testing according to the preset second statistic, and the second white blood cell test result is output; when the blood sample is judged to be a low-value white blood cell sample, the third statistic of the second sample to be tested is calculated using the preset white blood cell statistical particle number threshold, the second sample to be tested is pushed into the detection channel according to the calculated third statistic, and the third white blood cell test result is output; Among them, the third statistic is greater than or equal to the theoretical value of the third statistic The theoretical value of the third statistic is It is calculated by the following formula: ;in: The particle count threshold for the preset white blood cell count; The supply quantity of the sample to be tested reaches the preset first statistic Count the number of white blood cell particles at the time of The actual value of the third statistic It is calculated by the following formula: ;in: is the theoretical value of the third statistic; "[]" is the rounding function; is the minimum measurement value of the statistic; the third statistic is the actual value of the third statistic Or the theoretical value of the third statistic ; The second sample to be tested is calculated according to the actual value of the third statistic Or the theoretical value of the third statistic Push it into the detection channel and output the third white blood cell detection result; Before executing the white blood cell test of the blood sample, the start instruction of the first detection mode or the second detection mode is triggered by the user or automatically; the start instruction of the first detection mode or the second detection mode is automatically triggered, and when the current blood sample executes the second detection mode to complete the test and is a low-value white blood cell sample, the start instruction of the second detection mode is automatically triggered when executing the white blood cell test of the next blood sample; otherwise, the start instruction of the first detection mode is automatically triggered.

2. The dynamic detection method for leukocyte detection according to claim 1, characterized in that: The detection channel is an impedance detection channel or an optical detection channel, and / or the first sample to be tested and the second sample to be tested are the same or different.

3. The dynamic detection method for leukocyte detection according to claim 1, characterized in that: When the second detection mode is enabled, the method for determining whether the blood sample is a low-value white blood cell sample is as follows: the white blood cell concentration is calculated based on the number of white blood cell particles, and whether the white blood cell concentration is lower than a preset concentration threshold: if so, the blood sample is a low-value white blood cell sample; if not, the blood sample is not a low-value white blood cell sample.

4. The dynamic detection method for leukocyte detection according to claim 1, characterized in that: When the blood sample is determined to be a low-leukocyte sample, if the third statistic is greater than a preset fourth statistic, the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and a fourth leukocyte detection result is output.

5. The dynamic detection method for leukocyte detection according to claim 1, characterized in that: When the blood sample is judged to be a low-value white blood cell sample, if the third statistic is greater than the preset fourth statistic, a prompt message is output, or the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and the fourth white blood cell detection result and prompt message are output.

6. The dynamic detection method for leukocyte detection according to claim 5, characterized in that: When the blood sample is determined to be a low-leukocyte sample, if the third statistic is greater than a preset fourth statistic, the blood sample is retested.

7. The dynamic detection method for leukocyte detection according to claim 6, characterized in that: A third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

8. The dynamic detection method for leukocyte detection according to claim 1, characterized in that: The start instruction of the first detection mode or the second detection mode is triggered by the user. If the user triggers the start instruction of the first detection mode, the first detection mode is enabled to detect the blood sample; If the user triggers the start instruction of the second detection mode, the second detection mode is enabled to detect the blood sample.

9. The dynamic detection method for leukocyte detection according to claim 8, characterized in that: Before enabling the first detection mode or the second detection mode, it also includes: obtaining a click instruction of a visual operation interface, or a trigger instruction of a control panel; the visual operation interface displays the first detection mode option and the second detection mode option; the control panel is provided with a first detection mode button and a second detection mode button; wherein, enabling the first detection mode or the second detection mode includes: when the click instruction is a click instruction for the first detection mode option, or when the trigger instruction is a trigger instruction for the first detection mode button, enabling the first detection mode; when the click instruction is a click instruction for the second detection mode option, or when the trigger instruction is a trigger instruction for the second detection mode button, enabling the second detection mode.

10. A computer-readable storage medium, characterized in that: The invention comprises a program which can be executed by a processor to implement the dynamic detection method for leukocyte detection as claimed in any one of claims 1 to 9.

11. A sample detection device, comprising: Sampling distribution module, reagent supply unit, mixing chamber, detection module, power device and mode setting unit; The sampling distribution module is configured to collect a blood sample and supply the blood sample at least partially to the mixing chamber; the reagent supply unit is configured to supply reagents to the mixing chamber; the mixing chamber is configured to receive the blood sample distributed by the sampling distribution module and the reagents provided by the reagent supply unit to prepare a sample to be tested; The detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the test sample to the optical detection channel or the impedance detection channel; the mode setting unit is used to enable the first mode, or enable the second mode; the detection device also includes a processor, and the processor is used to execute the program in a computer-readable storage medium as described in claim 10.

12. A dynamic detection method for leukocyte detection, characterized in that: The method comprises: A second sample to be tested is prepared by using a blood sample, and the second sample to be tested is supplied to a detection channel by using a power device, and the second sample to be tested is first supplied to the detection channel for detection according to a preset first statistic, and a detection signal of the second sample to be tested is obtained, and the detection signal of the second sample to be tested is at least used to obtain a white blood cell detection result of the blood sample, and based on the white blood cell detection result of the second sample to be tested of the first statistic, it is determined whether the blood sample is a low-value white blood cell sample; When the blood sample is judged to be a non-low-value white blood cell sample, the second sample to be tested is supplied to the detection channel for testing according to the preset second statistic, and the second white blood cell test result is output; when the blood sample is judged to be a low-value white blood cell sample, the third statistic of the second sample to be tested is calculated using the preset white blood cell statistical particle number threshold, the second sample to be tested is pushed into the detection channel according to the calculated third statistic, and the third white blood cell test result is output; Among them, the third statistic is greater than or equal to the theoretical value of the third statistic The theoretical value of the third statistic is It is calculated by the following formula: ;in: The particle count threshold for the preset white blood cell count; The supply quantity of the sample to be tested reaches the preset first statistic Count the number of white blood cell particles at the time of The actual value of the third statistic It is calculated by the following formula: ;in: is the theoretical value of the third statistic; "[]" is the rounding function; is the minimum measurement value of the statistic; the third statistic is the actual value of the third statistic Or the theoretical value of the third statistic ; The second sample to be tested is calculated according to the actual value of the third statistic Or the theoretical value of the third statistic Push it into the detection channel and output the third white blood cell detection result.

13. The dynamic detection method for leukocyte detection according to claim 12, characterized in that: When the blood sample is determined to be a low-leukocyte sample, if the third statistic is greater than a preset fourth statistic, the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and a fourth leukocyte detection result is output.

14. The dynamic detection method for leukocyte detection according to claim 12, characterized in that: When the blood sample is judged to be a low-value white blood cell sample, if the third statistic is greater than the preset fourth statistic, a prompt message is output, or the second test sample is supplied to the detection channel for detection according to the preset fourth statistic, and the fourth white blood cell detection result and prompt message are output.

15. The dynamic detection method for leukocyte detection according to claim 12, characterized in that: When the blood sample is determined to be a low-leukocyte sample, if the third statistic is greater than a preset fourth statistic, the blood sample is retested.

16. The dynamic detection method for leukocyte detection according to claim 15, characterized in that: A third sample to be tested is prepared using the blood sample for retesting, and the concentration of the third sample to be tested is greater than the concentration of the second sample to be tested.

17. The dynamic detection method for leukocyte detection according to claim 12, characterized in that: The detection channel is an impedance detection channel and / or an optical detection channel.

18. A computer-readable storage medium, characterized in that: The invention comprises a program which can be executed by a processor to implement the dynamic detection method for leukocyte detection as claimed in any one of claims 12 to 17.

19. A sample detection device, comprising: Sampling distribution module, reagent supply unit, mixing chamber, detection module and power unit; The sampling distribution module is configured to collect a blood sample and supply the blood sample at least partially to the mixing chamber; the reagent supply unit is configured to supply reagents to the mixing chamber; the mixing chamber is configured to receive the blood sample distributed by the sampling distribution module and the reagents provided by the reagent supply unit to prepare a sample to be tested; The detection module is an optical detection module or an impedance detection module, the optical detection module has an optical detection channel, and the impedance detection module has an impedance detection channel; the power device is used to supply the test sample to the optical detection channel or the impedance detection channel; the detection device also includes a processor, and the processor is used to execute the program in a computer-readable storage medium as described in claim 18.

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