A method for breeding brussels sprouts double haploid by distant hybridization induced parthenogenesis

Abstract

The present invention discloses a method for cultivating double haploid of fragrant green vegetable by inducing parthenogenesis through distant hybridization. Using non-heading Chinese cabbage 'Fragrant Green Vegetable' as the female parent and broad bean 'Japanese Big White Skin' as the male parent, artificial pollination hybridization is carried out, and brassinolide solution is sprayed to induce parthenogenesis, so as to cultivate the double haploid of fragrant green vegetable. As long as artificial cross-pollination is carried out and combined with the application of brassinolide solution, the double haploid of fragrant green vegetable can be obtained, which can accelerate the rate of cultivating high-quality germplasm resources of fragrant green vegetable.

Claims

1. A method for breeding Brassica juncea double haploids by distant hybridization inducing parthenogenesis, characterized by, Using non-heading Chinese cabbage 'Xiangqingcai' as the female parent and broad bean 'Japanese White Skin' as the male parent, artificial pollination and hybridization were carried out, and brassinolide solution was sprayed to induce parthenogenesis, thereby cultivating double haploid Xiangqingcai. The method specifically includes the following steps: Step 1: Before flowering, the non-heading Chinese cabbage 'Xiangqingcai' is artificially deemasculated and sprayed with brassinolide solution, and then bagged for isolation; Step 2: On the day the Chinese broad bean flower treated in Step 1 blooms, select the male parent flower that has opened that day, pollinate it on the stigma of the female parent that has been emasculated, apply brassinolide solution to the young ovary of the female parent, and continue to isolate it by bagging. The concentration of the brassinolide solution applied by spraying and smearing is 0.0005% to 0.0006%.

2. The method for cultivating double haploids of *Clerodendrum trichotomum* by induced parthenogenesis through distant hybridization according to claim 1, characterized in that, Step 1 is performed two days before the mother plant flowers.

3. The method for cultivating double haploids of *Clerodendrum trichotomum* by induced parthenogenesis through distant hybridization according to claim 1, characterized in that, Step 2 is carried out between 9:00 and 10:00 on the day the coriander flowers.

4. The method for cultivating double haploids of *Clerodendrum trichotomum* by induced parthenogenesis through distant hybridization according to claim 1, characterized in that, In step 2, the stigma of a single emasculated female parent is pollinated using 2 to 3 broad bean flowers.

5. The method for cultivating double haploids of *Clerodendrum trichotomum* by induced parthenogenesis through distant hybridization according to claim 1, characterized in that, The variety of the fragrant green vegetable mentioned is embroidered tendon.

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