Micro-gel beads skin care product and preparation method thereof

Abstract

The present invention relates to the technical field of skin care products, and in particular to a micro-condensed bead skin care product and a preparation method thereof. The micro-condensed bead skin care product is composed of the following raw materials in percentage by mass: 4-12wt% glycerol, 1-4wt% 1,2-propylene glycol, 0.5-2wt% ceramide, 1-4wt% thickener, 1-3wt% behenyl alcohol, 2-4wt% vegetable oil, 0.05-0.3wt% sodium hyaluronate, 0.5-1wt% allantoin, 1-4wt% hydrogenated polyisobutylene, 3-7wt% plant fermentation, and the balance is water. The micro-condensed bead skin care product prepared by adding multi-functional plant fermentation has good whitening and moisturizing effects.

Description

Technical Field

[0001] The invention relates to the technical field of skin care products, and in particular to micro-condensed bead skin care products and a preparation method thereof. Background Art

[0002] Skin care products are a type of daily chemical industrial products for the purpose of cleaning, protecting, beautifying and modifying. They can be applied to the skin, hair, nails, lips and other human surfaces by rubbing, spraying or other similar methods. With the rapid development of my country's economy and the great changes in people's living standards, consumers' pursuit of beauty has become increasingly prominent. Especially in the past decade, driven by the Internet wave, the cosmetics industry has developed rapidly, and consumers' demand for cosmetics has shown a trend of becoming more refined, professional and personalized. Green, safe and efficient have become important characteristics of the future development of cosmetics.

[0003] Traditional Chinese medicine resources have distinct advantages in the field of cosmetics research and development due to their naturalness, safety, good efficacy, long history, and emphasis on the overall concept. Adding some suitable Chinese herbal medicines or active ingredients of Chinese herbal medicines to the production of beauty cosmetics, on the one hand, the effect is no worse than that of artificial synthetic substances, which can meet people's pursuit of a high-quality lifestyle; on the other hand, it is safer, less toxic and less polluting to the environment, which fits the consumers' pursuit of "returning to nature" on the basis of personalization, diversification and quality. Chinese herbal cosmetics have a long history of use and high safety. Chinese medicine has accumulated experience in clinical application scientific research and applied practice research of modern Chinese medicine products for thousands of years. The raw materials of Chinese medicine products come from nature, which are pure and mild, with little toxicity and side effects, green and natural, safe and reliable. Compared with chemical drugs, the use of Chinese medicine cosmetics is safer and more reliable.

[0004] Chinese invention patent CN109846792A discloses a micro-condensed bead moisturizing skin care product and a preparation method thereof, wherein the skin care product is mainly composed of the following components: 0.1-7% pentaerythritol tetra(ethylhexanoate), 0.005-0.05% acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer, 0.4-11% emollient, 0.02-0.6% anti-inflammatory agent, 8-27% moisturizing agent A, 0.01-0.15% chelating agent, 0.1-0.5% suspending agent, 0.01-0.14% pH regulator, 0.01-4% moisturizing agent B, 0.3-1.5% antioxidant, and appropriate amount of deionized water. Although the micro-condensed bead moisturizing skin care product prepared by the invention has a good moisturizing effect, the effect of the micro-condensed bead moisturizing skin care product prepared by the invention is relatively single, and it is difficult to meet the needs of the general public consumers. Summary of the invention

[0005] In view of the above-mentioned deficiencies in the prior art, the present invention provides a micro-gel bead skin care product and a preparation method thereof.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] A micro-gel bead skin care product is composed of the following raw materials in percentage by mass: 4-12wt% glycerol, 1-4wt% 1,2-propylene glycol, 0.5-2wt% ceramide, 1-4wt% thickener, 1-3wt% behenyl alcohol, 2-4wt% vegetable oil, 0.05-0.3wt% sodium hyaluronate, 0.5-1wt% allantoin, 1-4wt% hydrogenated polyisobutene, 3-7wt% plant fermentation product, and the balance is water.

[0008] The thickener is at least one of carbomer, hydroxyethyl acrylate, xanthan gum and cetearyl alcohol.

[0009] The vegetable oil is at least one of macadamia nut oil, sachalinensis oil, coconut oil, meadowfoam seed oil and sea buckthorn berry oil.

[0010] Gynura procumbens extract contains flavonoids, terpenoids, polysaccharides and organic acid active substances. Flavonoids are one of the main active ingredients in Gynura procumbens, including quercetin, kaempferol, isorhamnetin, etc. These compounds have multiple biological activities such as antioxidant and anti-inflammatory. Terpenoids are also relatively abundant in Gynura procumbens, including triterpenes and steroidal terpenes, which have anti-inflammatory, antibacterial and antiviral effects. Sarcandra glabra (Thunb.) Nakai contains flavonoids, coumarins, sesquiterpenes, polysaccharides and phenolic acid compounds. Among them, isoflavone in its coumarin components is considered to have certain antibacterial effects; polyphenolic component rosmarinic acid has antibacterial, anti-inflammatory and antiviral physiological activities; Sarcandra glabra crude polysaccharide has the ability to scavenge hydroxyl free radicals, that is, it has a good in vitro antioxidant effect. PinkBeauty is rich in flavonoids, terpenes, tannin compounds and other ingredients, and has certain anti-inflammatory, analgesic, whitening and antioxidant effects. PinkBeauty can inhibit the activity of tyrosinase in the reaction process and reduce the formation of melanin, thereby achieving a whitening effect.

[0011] In summary, the extracts of Panax notoginseng, Coral Grass and Potentilla fruticosa contain antioxidants such as flavonoids and rich nutrients, have good antioxidant effects, can reduce the damage of free radicals induced by ultraviolet rays and chemical substances to skin tissue, thereby playing a role in delaying aging, while the extracts of Panax notoginseng and Potentilla fruticosa can inhibit the activity of tyrosinase, prevent the formation of melanin, and whiten the skin; as a natural whitening agent derived from plants, it has the advantage of combining multiple ingredients and multiple functions, it improves skin color by promoting blood circulation, reduces the melanin content and thus plays a whitening role, protects skin color through antioxidant effects, and inhibits the proliferation of melanin by inhibiting the expression and activity of tyrosinase to achieve a whitening effect.

[0012] The method for preparing the plant fermentation product comprises the following steps:

[0013] S1: washing, drying, crushing, and sieving the plant raw materials to obtain plant powder, adding water and ultrasound, cooling, and obtaining a plant mixture;

[0014] S2 adding peptone and glucose to the plant mixture, then adding citric acid to adjust the pH, sterilizing, and obtaining a fermentation liquid;

[0015] S3: adding a fermentation synergist to the fermentation liquid, inoculating fermentation bacteria, and performing constant temperature fermentation to obtain a fermentation product;

[0016] S4: adding water of the same mass as the fermentation product to the above fermentation product, ripening, filtering, and freeze-drying the filtrate to obtain the plant fermentation product.

[0017] Although plant extracts are added to skin care products to produce a certain whitening and moisturizing effect on the skin, the utilization rate of active substances in the extraction process is far from enough, causing great waste. The present invention uses plants as fermentation raw materials, and adds plant lactobacillus and Kluyveromyces marxianus to ferment during the fermentation process to improve the utilization rate of active substances in plants. During the fermentation process, plant lactobacillus and Kluyveromyces marxianus can transform certain components in plants, such as flavonoids, so that they are more easily absorbed by the skin. During the fermentation process, plant cell walls can also be decomposed to release more active ingredients, improve their bioavailability on the skin, and thus enhance the whitening effect; during the fermentation process, microorganisms may produce moisturizing factors such as hyaluronic acid and polypeptides, which can help the skin retain moisture, and polysaccharides and other ingredients in the fermentation product may help enhance the barrier function of the skin and reduce water loss. Therefore, the product after fermentation by Kluyveromyces marxianus and plant lactobacillus can be used to prepare a product with moisturizing, improving skin gloss, and whitening effect, while also protecting the skin barrier function.

[0018] Preferably, the method for preparing the plant fermentation product comprises the following steps:

[0019] S1: washing, drying, crushing, and passing the plant raw materials through a 10-30 mesh sieve to obtain plant powder, adding water, and ultrasonicating for 0.5-2h at an ultrasonic power of 200-400W, a frequency of 50-70kHz, and a temperature of 80-100°C, and cooling to obtain a plant mixture;

[0020] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 2-4wt% of the plant mixture and the glucose is 10-20wt% of the plant mixture, and then adding citric acid to adjust the pH to 4.5-5.5, sterilizing, and obtaining a fermentation liquid;

[0021] S3: adding 2-4 wt% fermentation synergist to the fermentation liquid, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 3-6 wt%, and performing constant temperature fermentation at 30-40° C., stirring speed is 100-180 rpm, and fermentation is performed for 2-5 days to obtain a fermentation product;

[0022] S4: adding water of the same mass as the fermentation product to the above fermentation product, ripening at 90-100° C. for 1-2 hours, filtering, and freeze-drying the filtrate to obtain the plant fermentation product.

[0023] The plant raw material in S1 is at least one of Notoginseng procumbens, Coral Sarcandra, and Potentilla fruticosa; preferably, the plant raw material is a mixture of Notoginseng procumbens, Coral Sarcandra, and Potentilla fruticosa in a mass ratio of (2-4): (1-3): (1-3).

[0024] The mass ratio of the plant powder to water in the S1 is 1:(6-10).

[0025] Adding fermentation enhancers during the fermentation process can not only provide a more suitable growth environment for microorganisms, enhance the metabolic activity of microorganisms, and thus more effectively transform the active ingredients in plants and improve their utilization rate; it may also help destroy the plant cell walls, release more active ingredients, and make them easier for microorganisms to transform and utilize. Coenzyme Q10, as an antioxidant, can protect the active ingredients from being oxidized and destroyed during the fermentation process, and sodium alginate can improve the stability of the final product and reduce the loss of active ingredients during storage. The two work synergistically to improve their stability and utilization rate; certain metabolites produced during the fermentation process may help the absorption of active ingredients and improve their application effect in skin care products.

[0026] The fermentation enhancer in S3 is coenzyme Q10 and / or sodium alginate; preferably, the fermentation enhancer is a mixture of coenzyme Q10 and sodium alginate in a mass ratio of 1:(5-8).

[0027] The fermentation bacteria in S3 is at least one of Lactobacillus plantarum and Kluyveromyces marxianus. Preferably, the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of (1-2):1.

[0028] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0029] (1) Evenly mix water, allantoin, behenyl alcohol and sodium hyaluronate, and stir at 800-1200 rpm and 50-80° C. for 20-50 min to obtain a mixture A;

[0030] (2) Mix glycerin, 1,2-propylene glycol, thickener, vegetable oil, ceramide, hydrogenated polyisobutene and plant fermentation product and homogenize for 2-5 minutes until they are evenly dispersed without fine particles, stir at 100-300 rpm for 10-30 minutes, heat to 70-90°C and stir for 15-30 minutes, cool to room temperature, add the above mixture A, mix evenly, and obtain micro-gel beads skin care product.

[0031] Because the microemulsion droplets are both lipophilic and hydrophilic, they have good compatibility with the intercellular lipid bilayer of the stratum corneum. By increasing the fluidity of the stratum corneum lipid bilayer, the active ingredients in the essence can easily penetrate the stratum corneum to reach the epidermis, thereby better exerting the hydrating effect of the water phase and the moisturizing effect of the oil phase. At the same time, active ingredients with moisturizing effects can be added to the water phase, so that the active ingredients can penetrate into the skin with the water phase, and have a chemical moisturizing effect in the skin, which can greatly increase the transdermal rate of the active ingredients. The oil phase covers the outermost layer of the skin, has a physical moisturizing effect, and hinders the evaporation of the water phase components absorbed by the skin, so that the moisture is better locked in the skin. The active ingredients that penetrate into the skin with the water phase can also be retained in the skin for a long time to exert the moisturizing effect, so that the hydrating and moisturizing effects are better exerted, effectively prolonging the duration of hydrating and moisturizing, prolonging the action time of the functional substances, and improving their absorption efficiency.

[0032] Beneficial effects of the present invention: The present invention provides a micro-gel beads skin care product and a preparation method thereof. The present invention can effectively inhibit the activity of tyrosinase, improve dull skin, and enhance whitening effect by matching the natural effective ingredients in plant fermentation products with other ingredients in the raw materials. By reasonably controlling the amount of each component, each component can give full play to its respective role and cooperate with each other, so that the obtained micro-gel beads skin care product has significant effects of moisturizing, locking water, moisturizing, and whitening. The micro-gel beads skin care product of the present invention does not contain any ingredients harmful to the human body, is non-irritating to the skin, will not cause allergic reactions, and is a safe and effective micro-gel beads skin care product. DETAILED DESCRIPTION

[0033] The above content of the present invention is further described in detail below in conjunction with specific implementation modes, but this should not be understood as the scope of the above subject matter of the present invention being limited to the following embodiments.

[0034] Introduction of some raw materials in this application:

[0035] Carbomer, CAS: 9007-20-9, Carbomer 940, were purchased from Shaanxi Panier Biotechnology Co., Ltd.

[0036] Hydrogenated polyisobutylene, product number 111-01-3, was purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0037] Peptone, model: Y005A, was purchased from Beijing Hongrun Baoshun Technology Co., Ltd.

[0038] Sea buckthorn fruit oil, item number 090812, was purchased from Jiangxi Xuesong Natural Medicinal Oil Co., Ltd.

[0039] Coenzyme Q10, product number: C111044, was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0040] Sodium alginate, batch number 20210423, was purchased from Jiangsu Jiujia Biotechnology Co., Ltd.

[0041] Lactobacillus plantarum, strain number: CICC24435, was purchased from China Industrial Microbiology Culture Collection Center.

[0042] Kluyveromyces marxianus, strain number: ACCC20314, was purchased from China Agricultural Microbiological Culture Collection Center.

[0043] Example 1

[0044] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 10wt% glycerin, 2wt% 1,2-propylene glycol, 1wt% ceramide, 2wt% thickener, 2wt% behenyl alcohol, 3wt% vegetable oil, 0.1wt% sodium hyaluronate, 0.8wt% allantoin, 2wt% hydrogenated polyisobutene, 5wt% plant fermentation product, and the balance is water.

[0045] The thickener is carbomer.

[0046] The vegetable oil is sea buckthorn fruit oil.

[0047] The method for preparing the plant fermentation product comprises the following steps:

[0048] S1: washing, drying, crushing, and passing the plant raw materials through a 20-mesh sieve to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:8, ultrasonicating for 1 h at an ultrasonic power of 300 W, a frequency of 60 kHz, and a temperature of 90° C., and cooling to obtain a plant mixture; the plant raw materials are a mixture of notoginseng procumbentum, coral grass, and sweet potato in a mass ratio of 3:2:2;

[0049] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 3wt% of the plant mixture and the glucose is 15wt% of the plant mixture, and then adding citric acid to adjust the pH to 5, sterilizing, and obtaining a fermentation liquid;

[0050] S3: adding 3wt% fermentation synergist to the fermentation broth, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 5wt%, and the fermentation is carried out at a constant temperature of 35°C, with a stirring speed of 120rpm, and the fermentation is carried out for 3 days to obtain a fermentation product; the fermentation synergist is a mixture of coenzyme Q10 and sodium alginate in a mass ratio of 1:6; the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of 3:2;

[0051] S4: adding water of the same mass as the fermentation product to the above fermentation product, aging at 100° C. for 1.5 hours, filtering, and freeze-drying the filtrate to obtain a plant fermentation product.

[0052] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0053] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 1000 rpm and 70° C. for 30 min to obtain a mixture A;

[0054] (2) Mix glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene, and plant fermentation product and homogenize for 3 minutes until they are evenly dispersed without fine particles. Stir at 200 rpm for 15 minutes, heat to 80°C and stir for 20 minutes. Cool to room temperature, add the above mixture A, and mix well to obtain a micro-gel bead skin care product.

[0055] Example 2

[0056] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 4wt% glycerin, 1wt% 1,2-propylene glycol, 0.5wt% ceramide, 1wt% thickener, 1wt% behenyl alcohol, 2wt% vegetable oil, 0.05wt% sodium hyaluronate, 0.5wt% allantoin, 1wt% hydrogenated polyisobutene, 3wt% plant fermentation product, and the balance is water.

[0057] The thickener is carbomer.

[0058] The vegetable oil is sea buckthorn fruit oil.

[0059] The method for preparing the plant fermentation product comprises the following steps:

[0060] S1: washing, drying, crushing, and passing the plant raw materials through a 10-mesh sieve to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:6, ultrasonicating for 0.5 h at an ultrasonic power of 200 W, a frequency of 50 kHz, and a temperature of 80° C., and cooling to obtain a plant mixture; the plant raw materials are a mixture of Panax notoginseng, Coral Grass, and Potentilla fruticosa in a mass ratio of 2:1:1;

[0061] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 2wt% of the plant mixture and the glucose is 10wt% of the plant mixture, and then adding citric acid to adjust the pH to 4.5, sterilizing, and obtaining a fermentation liquid;

[0062] S3: adding 2wt% fermentation synergist to the fermentation broth, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 3wt%, and the fermentation is carried out at a constant temperature of 30°C, with a stirring speed of 100rpm, and the fermentation is carried out for 2 days to obtain a fermentation product; the fermentation synergist is a mixture of coenzyme Q10 and sodium alginate in a mass ratio of 1:5; the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of 1:1;

[0063] S4: adding water of the same mass as the fermentation product to the above fermentation product, ripening at 90°C for 1 hour, filtering, and freeze-drying the filtrate to obtain a plant fermentation product.

[0064] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0065] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 800 rpm and 50° C. for 20 min to obtain a mixture A;

[0066] (2) Mix glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene, and plant fermentation product and homogenize for 2 minutes until they are evenly dispersed without fine particles. Stir at 100 rpm for 10 minutes, heat to 70°C and stir for 15 minutes. Cool to room temperature, add the above mixture A, and mix well to obtain a micro-gel bead skin care product.

[0067] Example 3

[0068] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 12wt% glycerin, 4wt% 1,2-propylene glycol, 2wt% ceramide, 4wt% thickener, 3wt% behenyl alcohol, 4wt% vegetable oil, 0.3wt% sodium hyaluronate, 1wt% allantoin, 4wt% hydrogenated polyisobutene, 7wt% plant fermentation product, and the balance is water.

[0069] The thickener is carbomer.

[0070] The vegetable oil is sea buckthorn fruit oil.

[0071] The method for preparing the plant fermentation product comprises the following steps:

[0072] S1: washing, drying, crushing, and passing the plant raw materials through a 30-mesh sieve to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:10, ultrasonicating for 2 hours at an ultrasonic power of 400 W, a frequency of 70 kHz, and a temperature of 100° C., and cooling to obtain a plant mixture; the plant raw materials are a mixture of notoginseng procumbentum, coral grass, and sweet potato in a mass ratio of 4:3:3;

[0073] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 4wt% of the plant mixture and the glucose is 20wt% of the plant mixture, and then adding citric acid to adjust the pH to 5.5, sterilizing, and obtaining a fermentation liquid;

[0074] S3: adding 3wt% fermentation synergist to the fermentation broth, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 6wt%, and the fermentation is carried out at a constant temperature of 40°C, with a stirring speed of 180rpm, and fermentation is carried out for 5 days to obtain a fermentation product; the fermentation synergist is a mixture of coenzyme Q10 and sodium alginate in a mass ratio of 1:8; the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of 2:1;

[0075] S4: adding water of the same mass as the fermentation product to the above fermentation product, aging at 100° C. for 2 hours, filtering, and freeze-drying the filtrate to obtain a plant fermentation product.

[0076] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0077] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 1200 rpm and 80° C. for 50 min to obtain a mixture A;

[0078] (2) Mix glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene, and plant fermentation product and homogenize for 5 minutes until they are evenly dispersed without fine particles. Stir at 300 rpm for 30 minutes, heat to 90°C and stir for 30 minutes. Cool to room temperature, add the above mixture A, and mix well to obtain a micro-gel bead skin care product.

[0079] Comparative Example 1

[0080] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 10wt% glycerin, 2wt% 1,2-propylene glycol, 1wt% ceramide, 2wt% thickener, 2wt% behenyl alcohol, 3wt% vegetable oil, 0.1wt% sodium hyaluronate, 0.8wt% allantoin, 2wt% hydrogenated polyisobutene, 5wt% plant extract, and the balance is water.

[0081] The thickener is carbomer.

[0082] The vegetable oil is sea buckthorn fruit oil.

[0083] The preparation method of the plant extract comprises the following steps: washing, drying, crushing, and passing a 20-mesh sieve through a plant raw material to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:8, ultrasonicating for 1 hour at an ultrasonic power of 300 W, a frequency of 60 kHz, and a temperature of 90° C., filtering, and freeze-drying the filtrate to obtain a plant extract. The plant raw material is a mixture of notoginseng flatus, coral grass, and sweet potato in a mass ratio of 3:2:2.

[0084] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0085] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 1000 rpm and 70° C. for 30 min to obtain a mixture A;

[0086] (2) Glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene and plant extract were mixed and homogenized for 3 minutes until they were evenly dispersed without fine particles, stirred at 200 rpm for 15 minutes, heated to 80°C and stirred for 20 minutes, cooled to room temperature, added to the above mixture A, and mixed evenly to obtain micro-gel beads skin care product.

[0087] Comparative Example 2

[0088] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 10wt% glycerin, 2wt% 1,2-propylene glycol, 1wt% ceramide, 2wt% thickener, 2wt% behenyl alcohol, 3wt% vegetable oil, 0.1wt% sodium hyaluronate, 0.8wt% allantoin, 2wt% hydrogenated polyisobutene, 5wt% plant fermentation product, and the balance is water.

[0089] The thickener is carbomer.

[0090] The vegetable oil is sea buckthorn fruit oil.

[0091] The method for preparing the plant fermentation product comprises the following steps:

[0092] S1: washing, drying, crushing, and passing the plant raw materials through a 20-mesh sieve to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:8, ultrasonicating for 1 h at an ultrasonic power of 300 W, a frequency of 60 kHz, and a temperature of 90° C., and cooling to obtain a plant mixture; the plant raw materials are a mixture of notoginseng procumbentum, coral grass, and sweet potato in a mass ratio of 3:2:2;

[0093] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 3wt% of the plant mixture and the glucose is 15wt% of the plant mixture, and then adding citric acid to adjust the pH to 5, sterilizing, and obtaining a fermentation liquid;

[0094] S3 inoculating fermentation bacteria in the fermentation liquid, wherein the inoculation amount of the fermentation bacteria is 5wt%, and the fermentation is carried out at a constant temperature of 35°C, with a stirring speed of 120rpm, and the fermentation is carried out for 3 days to obtain a fermentation product; the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of 3:2;

[0095] S4: adding water of the same mass as the fermentation product to the above fermentation product, aging at 100° C. for 1.5 hours, filtering, and freeze-drying the filtrate to obtain a plant fermentation product.

[0096] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0097] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 1000 rpm and 70° C. for 30 min to obtain a mixture A;

[0098] (2) Mix glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene, and plant fermentation product and homogenize for 3 minutes until they are evenly dispersed without fine particles. Stir at 200 rpm for 15 minutes, heat to 80°C and stir for 20 minutes. Cool to room temperature, add the above mixture A, and mix well to obtain a micro-gel bead skin care product.

[0099] Comparative Example 3

[0100] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 10wt% glycerin, 2wt% 1,2-propylene glycol, 1wt% ceramide, 2wt% thickener, 2wt% behenyl alcohol, 3wt% vegetable oil, 0.1wt% sodium hyaluronate, 0.8wt% allantoin, 2wt% hydrogenated polyisobutene, 5wt% plant fermentation product, and the balance is water.

[0101] The thickener is carbomer.

[0102] The vegetable oil is sea buckthorn fruit oil.

[0103] The method for preparing the plant fermentation product comprises the following steps:

[0104] S1: washing, drying, crushing, and passing the plant raw materials through a 20-mesh sieve to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:8, ultrasonicating for 1 h at an ultrasonic power of 300 W, a frequency of 60 kHz, and a temperature of 90° C., and cooling to obtain a plant mixture; the plant raw materials are a mixture of notoginseng procumbentum, coral grass, and sweet potato in a mass ratio of 3:2:2;

[0105] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 3wt% of the plant mixture and the glucose is 15wt% of the plant mixture, and then adding citric acid to adjust the pH to 5, sterilizing, and obtaining a fermentation liquid;

[0106] S3: adding 3wt% fermentation synergist to the fermentation liquid, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 5wt%, and the fermentation is carried out at a constant temperature of 35°C, with a stirring speed of 120rpm, and fermentation is carried out for 3 days to obtain a fermentation product; the fermentation synergist is coenzyme Q10; and the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of 3:2;

[0107] S4: adding water of the same mass as the fermentation product to the above fermentation product, aging at 100° C. for 1.5 hours, filtering, and freeze-drying the filtrate to obtain a plant fermentation product.

[0108] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0109] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 1000 rpm and 70° C. for 30 min to obtain a mixture A;

[0110] (2) Mix glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene, and plant fermentation product and homogenize for 3 minutes until they are evenly dispersed without fine particles. Stir at 200 rpm for 15 minutes, heat to 80°C and stir for 20 minutes. Cool to room temperature, add the above mixture A, and mix well to obtain a micro-gel bead skin care product.

[0111] Comparative Example 4

[0112] The micro-gel beads skin care product is composed of the following raw materials in percentage by mass: 10wt% glycerin, 2wt% 1,2-propylene glycol, 1wt% ceramide, 2wt% thickener, 2wt% behenyl alcohol, 3wt% vegetable oil, 0.1wt% sodium hyaluronate, 0.8wt% allantoin, 2wt% hydrogenated polyisobutene, 5wt% plant fermentation product, and the balance is water.

[0113] The thickener is carbomer.

[0114] The vegetable oil is sea buckthorn fruit oil.

[0115] The method for preparing the plant fermentation product comprises the following steps:

[0116] S1: washing, drying, crushing, and passing the plant raw materials through a 20-mesh sieve to obtain plant powder, adding water, wherein the mass ratio of the plant powder to water is 1:8, ultrasonicating for 1 h at an ultrasonic power of 300 W, a frequency of 60 kHz, and a temperature of 90° C., and cooling to obtain a plant mixture; the plant raw materials are a mixture of notoginseng procumbentum, coral grass, and sweet potato in a mass ratio of 3:2:2;

[0117] S2: adding peptone and glucose to the plant mixture, wherein the peptone is 3wt% of the plant mixture and the glucose is 15wt% of the plant mixture, and then adding citric acid to adjust the pH to 5, sterilizing, and obtaining a fermentation liquid;

[0118] S3: adding 3wt% fermentation synergist to the fermentation liquid, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 5wt%, and the fermentation is carried out at a constant temperature of 35°C, with a stirring speed of 120rpm, and fermentation is carried out for 3 days to obtain a fermentation product; the fermentation synergist is sodium alginate; and the fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of 3:2;

[0119] S4: adding water of the same mass as the fermentation product to the above fermentation product, aging at 100° C. for 1.5 hours, filtering, and freeze-drying the filtrate to obtain a plant fermentation product.

[0120] The preparation method of the micro-condensed bead skin care product comprises the following steps:

[0121] (1) water, allantoin, behenyl alcohol and sodium hyaluronate were uniformly mixed and stirred at 1000 rpm and 70° C. for 30 min to obtain a mixture A;

[0122] (2) Mix glycerin, 1,2-propylene glycol, carbomer, sea buckthorn fruit oil, ceramide, hydrogenated polyisobutene, and plant fermentation product and homogenize for 3 minutes until they are evenly dispersed without fine particles. Stir at 200 rpm for 15 minutes, heat to 80°C and stir for 20 minutes. Cool to room temperature, add the above mixture A, and mix well to obtain a micro-gel bead skin care product.

[0123] Test Example 1

[0124] Tyrosinase activity inhibition test: 5 g of the plant fermentation products prepared in Examples 1-3 and Comparative Examples 1-4 were respectively taken, dissolved in a phosphate buffer solution with a pH of 6.8, and then prepared into test samples with a concentration of 1 mg / mL for testing.

[0125] Take 2 mL of the test sample and add 100 U of tyrosinase, heat it in a 37°C constant temperature water bath for 10 min, then add 1 mL of 0.003 mol / L DOPA reaction solution, stir thoroughly, react in a 37°C constant temperature water bath for 2 min, take it out and measure the absorbance at 475 nm. Each group was tested 5 times in parallel.

[0126] Inhibition rate = {[(AB)-(CD)] / (AB)}×100%.

[0127] Wherein, A is the absorbance of the mixed solution without sample and tyrosinase measured at 475nm; B is the absorbance of the mixed solution without sample and tyrosinase measured at 475nm; C is the absorbance of the mixed solution with sample and tyrosinase measured at 475nm; D is the absorbance of the mixed solution with sample but no tyrosinase measured at 475nm, the results are shown in Table 1;

[0128]

[0129] Test Example 2

[0130] Moisturizing performance test: 120 women aged between 20 and 40 were selected for skin moisturizing performance test. The subjects were divided into 6 groups, with 20 people in each group. The 5 groups of subjects were respectively smeared with the micro-gel beads skin care products prepared by Example 1 and Comparative Examples 1-4, and the control group was the micro-gel beads skin care products without the addition of the plant fermentation prepared by the present invention.

[0131] Moisturizing performance test: The subjects uniformly cleaned their facial skin and wiped it with a dry face towel. After 30 minutes, the moisture of the subjects' faces was measured and recorded using a HONKON-TC02s skin tester. The micro-gel beads skin care products of the above-mentioned embodiment 1, comparative examples 1-4 and the control group were used on each subject, and the moisture of the subjects' faces was measured and recorded after 1 hour and 4 hours of use, respectively. The moisture growth rate was calculated = (moisture after the test - moisture before the test) / moisture before the test × 100%. The results are shown in Table 2;

[0132]

[0133] It can be seen from the above results that the micro-gel beads skin care products prepared by the present invention have good moisturizing and whitening effects and are deeply loved by the masses. The micro-gel beads skin care products added with plant fermentation have better whitening and moisturizing effects. The reason is that the extracts of Flos Chrysanthemi, Coral Grass, and Herba Pieris contain antioxidant substances such as flavonoids and rich nutrients, which have good antioxidant effects, can reduce the damage of free radicals induced by ultraviolet rays and chemical substances to skin tissues, thereby playing a role in delaying aging. At the same time, the extracts of Flos Chrysanthemi and Herba Pieris can inhibit the activity of tyrosinase, prevent the formation of melanin, and whiten the skin; as a natural whitening agent derived from plants, it has the advantage of combining multiple components and multiple functions, improving skin color by promoting blood circulation, reducing melanin content, and thus playing a whitening role, protecting skin color through antioxidant effects, and inhibiting the proliferation of melanin by inhibiting the expression and activity of tyrosinase to achieve a whitening effect.

[0134] As can be seen from Example 1 and the comparative example, although plant extracts are added to skin care products to produce certain whitening and moisturizing effects on the skin, the utilization rate of active substances in the extraction process is far from enough, causing great waste. The present invention uses plants as fermentation raw materials, and adds plant lactobacillus and Kluyveromyces marxianus to ferment during the fermentation process to improve the utilization rate of active substances in plants. During the fermentation process, plant lactobacillus and Kluyveromyces marxianus can transform certain components in plants, such as flavonoids, so that they are more easily absorbed by the skin, and can also decompose plant cell walls during the fermentation process, release more active ingredients, improve their bioavailability on the skin, thereby enhancing the whitening effect; during the fermentation process, microorganisms may produce moisturizing factors such as hyaluronic acid and polypeptides, which can help the skin retain moisture, and polysaccharides and other ingredients in the fermentation product may help to enhance the barrier function of the skin and reduce water loss. Therefore, the product after fermentation by Kluyveromyces marxianus and plant lactobacillus can be used to prepare a product with moisturizing, improving skin gloss, and whitening effect, while also protecting the skin barrier function.

[0135] Adding fermentation enhancers during the fermentation process can not only provide a more suitable growth environment for microorganisms, enhance the metabolic activity of microorganisms, and thus more effectively transform the active ingredients in plants and improve their utilization rate; it may also help destroy the plant cell walls, release more active ingredients, and make them easier for microorganisms to transform and utilize. Coenzyme Q10, as an antioxidant, can protect the active ingredients from being oxidized and destroyed during the fermentation process, and sodium alginate can improve the stability of the final product and reduce the loss of active ingredients during storage. The two work synergistically to improve their stability and utilization rate; certain metabolites produced during the fermentation process may help the absorption of active ingredients and improve their application effect in skin care products.

Claims

1. A micro-gel beads skin care product, characterized in that: The composition is composed of the following raw materials in percentage by weight: 4-12wt% glycerol, 1-4wt% 1,2-propylene glycol, 0.5-2wt% ceramide, 1-4wt% thickener, 1-3wt% behenyl alcohol, 2-4wt% vegetable oil, 0.05-0.3wt% sodium hyaluronate, 0.5-1wt% allantoin, 1-4wt% hydrogenated polyisobutene, 3-7wt% plant fermentation product, and the balance is water; The method for preparing the plant fermentation product comprises the following steps: S1: washing, drying, crushing, and passing the plant raw materials through a 10-30 mesh sieve to obtain plant powder, adding water, and ultrasonicating for 0.5-2h at an ultrasonic power of 200-400W, a frequency of 50-70kHz, and a temperature of 80-100°C, and cooling to obtain a plant mixture; S2: adding peptone and glucose to the plant mixture, wherein the peptone is 2-4wt% of the plant mixture and the glucose is 10-20wt% of the plant mixture, and then adding citric acid to adjust the pH to 4.5-5.5, sterilizing, and obtaining a fermentation liquid; S3: adding 2-4 wt% fermentation synergist to the fermentation liquid, and then inoculating fermentation bacteria, wherein the inoculation amount of the fermentation bacteria is 3-6 wt%, and performing constant temperature fermentation at 30-40° C., stirring speed is 100-180 rpm, and fermentation is performed for 2-5 days to obtain a fermentation product; S4. Add water of the same mass as the fermentation product to the fermentation product, mature at 90-100° C. for 1-2 hours, filter, and freeze-dry the filtrate to obtain a plant fermentation product; The plant raw material is a mixture of Panax notoginseng procumbens, Coral Grass, and Potentilla fruticosa in a mass ratio of (2-4): (1-3): (1-3); The mass ratio of the plant powder to water in S1 is 1:(6-10); The fermentation enhancer is a mixture of coenzyme Q10 and sodium alginate in a mass ratio of 1:(5-8); The fermentation bacteria is a mixture of Lactobacillus plantarum and Kluyveromyces marxianus in a mass ratio of (1-2):

1.

2. The micro-gel beads skin care product according to claim 1, characterized in that: The thickener is at least one of carbomer, hydroxyethyl acrylate, xanthan gum and cetearyl alcohol.

3. The micro-gel beads skin care product according to claim 1, characterized in that: The vegetable oil is at least one of macadamia nut oil, sachalinensis oil, coconut oil, meadowfoam seed oil and sea buckthorn berry oil.

4. A method for preparing the micro-gel beads skin care product according to any one of claims 1 to 3, characterized in that: The steps include: (1) Evenly mix water, allantoin, behenyl alcohol and sodium hyaluronate, and stir at 800-1200 rpm and 50-80° C. for 20-50 min to obtain a mixture A; (2) Mix glycerin, 1,2-propylene glycol, thickener, vegetable oil, ceramide, hydrogenated polyisobutene and plant fermentation product and homogenize for 2-5 minutes until they are evenly dispersed without fine particles, stir at 100-300 rpm for 10-30 minutes, heat to 70-90°C and stir for 15-30 minutes, cool to room temperature, add the above mixture A, mix evenly, and obtain micro-gel beads skin care product.

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